CmaCh15G010620 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh15G010620
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionMembrane-associated kinase regulator 5
LocationCma_Chr15: 6743489 .. 6744782 (+)
RNA-Seq ExpressionCmaCh15G010620
SyntenyCmaCh15G010620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTCTCTCTCTCTCTCTATAAAACCCATCATTTTTCTCTGCATTTCCTATCTCATCTCACTATGGATTCCGTCGCCTATCTCAAGTTCTTCAAATTTTCCGGCGCCGATGGACTTCCAAAACAAGAACAAGACGAAGAAGAAGAAGAAGAAGAAGAAGATTCGTTCTTCGATCTCGAGCTTCTCTCTGTTTCCGCCGGCAAAATTCAAGGCCCAGAACAGAGGCCTCAAATTCCGACCAAATTAGGCGAACACATGAACCTCTGTTCCGATTCCATTTCGAAACGGAAGATTCTTCCAATCGAGCCCACTTCGAAGCCCCAATCTCCGATTCAATTTCTCAAATCAGCTCCCAAATTCACTGATTTCATCTTCAAGAAACAGAGATCAATGGCCAAGAACAGAGCACCCGCTGGCCCAGAACAAGAAGAAACCCGAATTCTAAGCTCGAAATTTACCAGAGACAACAGCACAAGAAGAATCAGAGGCATTTTTCCACCGGACCCATATGGGGTTCCGGCTCCATCTCCGGCGTGGCGGTTCTCGAAGGAGGCGGTGCAGAAATATCTAAAGCTGATAAAGCCTAAGCTTCTGAAGAAACCCACCGACAGCGGCGGCGAGTTGTCGTCCGCAGCTGTGGATTCTCCGGTGAGGGAGAAGCTCGAGAATGGTCTTCCGACAGGGATTAGGTTGGTCTGTAAGCATCTGGGTAGGAGCAAATCGGCCTCGGCGGCTAGCAGATTGGCGGCGGCAGTGGCGGTGGCTCCTCCACCGAACCGGAGGGATGATTCGTTATTACAGCTACACGACGGGATTGAAAGCGCCATTTTACACTGTAAAAGATCCTTGAATGCTTCCACAGGTAAAAACAAAGAAAGAAACCTCTTTTAAACTTATAATTAGGCCATTGATTTTTTTGAATTATGTTTTTGTGGTCAGAGAAACCTGTGTTGGCTATGGACGTCTGAGAAAGGTATTTTAGAGTATTATGTTCTTGTTCGATTCTATTTTCCGCCATTGATTTGGAAGCAAAGAGCTTCAAAATAATAAAATCTGAGAGAAACCCAGAAGAGGAAGAACAGTGAAAAAGAAGAGAAATTGTTGGGTGATGAAAGTGATGATGAACAATACAAATGGGGTTTGGGAGTTGGGAATATGTAAAAGGCTCATAATTATTTATCAAAAGGAATTTTAAAAGATATCTGATTCTGAAATTTGTCTGTGTTGGGATTTTGTCTCATGCAAACTCCAACCACTGTTTTATATATATATATATTTATATGAATGTCGAGATA

mRNA sequence

CTCTCTCTCTCTCTCTCTATAAAACCCATCATTTTTCTCTGCATTTCCTATCTCATCTCACTATGGATTCCGTCGCCTATCTCAAGTTCTTCAAATTTTCCGGCGCCGATGGACTTCCAAAACAAGAACAAGACGAAGAAGAAGAAGAAGAAGAAGAAGATTCGTTCTTCGATCTCGAGCTTCTCTCTGTTTCCGCCGGCAAAATTCAAGGCCCAGAACAGAGGCCTCAAATTCCGACCAAATTAGGCGAACACATGAACCTCTGTTCCGATTCCATTTCGAAACGGAAGATTCTTCCAATCGAGCCCACTTCGAAGCCCCAATCTCCGATTCAATTTCTCAAATCAGCTCCCAAATTCACTGATTTCATCTTCAAGAAACAGAGATCAATGGCCAAGAACAGAGCACCCGCTGGCCCAGAACAAGAAGAAACCCGAATTCTAAGCTCGAAATTTACCAGAGACAACAGCACAAGAAGAATCAGAGGCATTTTTCCACCGGACCCATATGGGGTTCCGGCTCCATCTCCGGCGTGGCGGTTCTCGAAGGAGGCGGTGCAGAAATATCTAAAGCTGATAAAGCCTAAGCTTCTGAAGAAACCCACCGACAGCGGCGGCGAGTTGTCGTCCGCAGCTGTGGATTCTCCGGTGAGGGAGAAGCTCGAGAATGGTCTTCCGACAGGGATTAGGTTGGTCTGTAAGCATCTGGGTAGGAGCAAATCGGCCTCGGCGGCTAGCAGATTGGCGGCGGCAGTGGCGGTGGCTCCTCCACCGAACCGGAGGGATGATTCGTTATTACAGCTACACGACGGGATTGAAAGCGCCATTTTACACTGTAAAAGATCCTTGAATGCTTCCACAGAGAAACCTGTGTTGGCTATGGACGTCTGAGAAAGGTATTTTAGAGTATTATGTTCTTGTTCGATTCTATTTTCCGCCATTGATTTGGAAGCAAAGAGCTTCAAAATAATAAAATCTGAGAGAAACCCAGAAGAGGAAGAACAGTGAAAAAGAAGAGAAATTGTTGGGTGATGAAAGTGATGATGAACAATACAAATGGGGTTTGGGAGTTGGGAATATGTAAAAGGCTCATAATTATTTATCAAAAGGAATTTTAAAAGATATCTGATTCTGAAATTTGTCTGTGTTGGGATTTTGTCTCATGCAAACTCCAACCACTGTTTTATATATATATATATTTATATGAATGTCGAGATA

Coding sequence (CDS)

ATGGATTCCGTCGCCTATCTCAAGTTCTTCAAATTTTCCGGCGCCGATGGACTTCCAAAACAAGAACAAGACGAAGAAGAAGAAGAAGAAGAAGAAGATTCGTTCTTCGATCTCGAGCTTCTCTCTGTTTCCGCCGGCAAAATTCAAGGCCCAGAACAGAGGCCTCAAATTCCGACCAAATTAGGCGAACACATGAACCTCTGTTCCGATTCCATTTCGAAACGGAAGATTCTTCCAATCGAGCCCACTTCGAAGCCCCAATCTCCGATTCAATTTCTCAAATCAGCTCCCAAATTCACTGATTTCATCTTCAAGAAACAGAGATCAATGGCCAAGAACAGAGCACCCGCTGGCCCAGAACAAGAAGAAACCCGAATTCTAAGCTCGAAATTTACCAGAGACAACAGCACAAGAAGAATCAGAGGCATTTTTCCACCGGACCCATATGGGGTTCCGGCTCCATCTCCGGCGTGGCGGTTCTCGAAGGAGGCGGTGCAGAAATATCTAAAGCTGATAAAGCCTAAGCTTCTGAAGAAACCCACCGACAGCGGCGGCGAGTTGTCGTCCGCAGCTGTGGATTCTCCGGTGAGGGAGAAGCTCGAGAATGGTCTTCCGACAGGGATTAGGTTGGTCTGTAAGCATCTGGGTAGGAGCAAATCGGCCTCGGCGGCTAGCAGATTGGCGGCGGCAGTGGCGGTGGCTCCTCCACCGAACCGGAGGGATGATTCGTTATTACAGCTACACGACGGGATTGAAAGCGCCATTTTACACTGTAAAAGATCCTTGAATGCTTCCACAGAGAAACCTGTGTTGGCTATGGACGTCTGA

Protein sequence

MDSVAYLKFFKFSGADGLPKQEQDEEEEEEEEDSFFDLELLSVSAGKIQGPEQRPQIPTKLGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAGPEQEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKPTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRLAAAVAVAPPPNRRDDSLLQLHDGIESAILHCKRSLNASTEKPVLAMDV
Homology
BLAST of CmaCh15G010620 vs. ExPASy Swiss-Prot
Match: Q9FLX4 (Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.9e-18
Identity = 83/264 (31.44%), Postives = 125/264 (47.35%), Query Frame = 0

Query: 13  SGADGLPKQEQDEEEEEEEEDSFFDLELLSVSAGKIQGPEQRPQIPTKLGEHMNLCSDSI 72
           S  D     E  + +  E EDSFF+L+   +S    + P+++ ++ T         S   
Sbjct: 28  SAVDSTTGSEDPDLDLCEGEDSFFELK---ISLSDFKTPKEKQRLETTTTTTTYSVS--- 87

Query: 73  SKRKILP-IEPTSKP-QSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAGPEQEETRI-LSS 132
           +K K+LP ++ +SKP QSP   LKS  KF  F FKK     K+      ++E  R  L+ 
Sbjct: 88  NKSKVLPFVDISSKPQQSPTTLLKSGQKFRAFSFKKSE---KSTTTEKKKEENNRTSLNV 147

Query: 133 KFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKPTDSGGELSS 192
           +F  D+ T                 +    F     +++  LIKP   K          +
Sbjct: 148 RFRVDDET-TTTSFRKTASIARSQQTDDTMFDDSVSKRFFSLIKPLYTKSTKKQSSSTIT 207

Query: 193 AAVDSP-VREKLENGLPTGIRLVCKHLGRSKSASAASRLAAAVAVAPPPNRRDDSLLQLH 252
           +   SP  REK  + +P+G+R V + LG+S+SAS      AA+    P NR D+SL    
Sbjct: 208 SPTSSPATREKQRSNIPSGMRSVRRQLGKSRSAS------AAIGGMSPANRIDESLQVQQ 267

Query: 253 DGIESAILHCKRSLNASTEKPVLA 273
           DGI+SAILHCK+S + S E  +L+
Sbjct: 268 DGIQSAILHCKKSFHGSRESSLLS 275

BLAST of CmaCh15G010620 vs. ExPASy Swiss-Prot
Match: Q9SH58 (Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 GN=MAKR2 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.6e-13
Identity = 50/128 (39.06%), Postives = 68/128 (53.12%), Query Frame = 0

Query: 165 VQKYLKLIKPKLLKKPTDSGGEL--------------SSAAVDSPVREKLEN------GL 224
           +QKYLK +KP  ++     G +L              ++   DSPV  K  N       +
Sbjct: 267 MQKYLKKVKPLYIRVSRRYGEKLRHSGQLTAPSSARSTAEKADSPVATKTNNKPGNNINI 326

Query: 225 PTGIRLVCKHLGRSKSASAA----SRLAAAVAVAPPPNRRDDSLLQLHDGIESAILHCKR 269
           P G+++V KHLG+S+S+S+     S   A V       RRDDSLLQ  D I+SAILHCKR
Sbjct: 327 PAGLKVVRKHLGKSRSSSSTATTPSSTTATVTTQSESRRRDDSLLQQQDSIQSAILHCKR 386

BLAST of CmaCh15G010620 vs. ExPASy TrEMBL
Match: A0A6J1IDF3 (probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=LOC111471559 PE=4 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 5.6e-150
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLPKQEQDEEEEEEEEDSFFDLELLSVSAGKIQGPEQRPQIPTK 60
           MDSVAYLKFFKFSGADGLPKQEQDEEEEEEEEDSFFDLELLSVSAGKIQGPEQRPQIPTK
Sbjct: 1   MDSVAYLKFFKFSGADGLPKQEQDEEEEEEEEDSFFDLELLSVSAGKIQGPEQRPQIPTK 60

Query: 61  LGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAGPE 120
           LGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAGPE
Sbjct: 61  LGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAGPE 120

Query: 121 QEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKP 180
           QEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKP
Sbjct: 121 QEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKP 180

Query: 181 TDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRLAAAVAVAPPPNRR 240
           TDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRLAAAVAVAPPPNRR
Sbjct: 181 TDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRLAAAVAVAPPPNRR 240

Query: 241 DDSLLQLHDGIESAILHCKRSLNASTEKPVLAMDV 276
           DDSLLQLHDGIESAILHCKRSLNASTEKPVLAMDV
Sbjct: 241 DDSLLQLHDGIESAILHCKRSLNASTEKPVLAMDV 275

BLAST of CmaCh15G010620 vs. ExPASy TrEMBL
Match: A0A6J1E2K7 (probable membrane-associated kinase regulator 5 OS=Cucurbita moschata OX=3662 GN=LOC111430217 PE=4 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.0e-135
Identity = 257/276 (93.12%), Postives = 260/276 (94.20%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLPKQEQDEEEEEEEEDSFFDLELLSVSAGKIQGPEQRPQIPTK 60
           MDSVAYLKFFKF+GADGL KQEQ EEEEEEEEDS FDLEL SVSAG IQ PEQRPQIPTK
Sbjct: 1   MDSVAYLKFFKFAGADGLLKQEQ-EEEEEEEEDSLFDLELFSVSAGGIQSPEQRPQIPTK 60

Query: 61  LGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAGPE 120
           LGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRS AKNRAPAG E
Sbjct: 61  LGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSTAKNRAPAGTE 120

Query: 121 QEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKP 180
           QEETRILSSKFTRDNSTRRIRGIFPPDP GVPAPSPAWRFSKEAVQKYLKLIKPKLLKKP
Sbjct: 121 QEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKP 180

Query: 181 TDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRL-AAAVAVAPPPNR 240
           TD+G ELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASA S L AAAVAVAPPPNR
Sbjct: 181 TDTGSELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASATSGLAAAAVAVAPPPNR 240

Query: 241 RDDSLLQLHDGIESAILHCKRSLNASTEKPVLAMDV 276
           RDDSLLQ HDGIESAILHCKRSLN STEKPVL +DV
Sbjct: 241 RDDSLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV 275

BLAST of CmaCh15G010620 vs. ExPASy TrEMBL
Match: A0A0A0KK03 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139900 PE=4 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 4.6e-112
Identity = 216/271 (79.70%), Postives = 234/271 (86.35%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLPKQEQDEEEEEEE-EDSFFDLELLSVSAGKIQGPEQRPQIPT 60
           MDSVA+LKFFK SGAD L KQE++EEEEEEE EDSFFD+E  SVSAGKIQ PEQRPQIPT
Sbjct: 1   MDSVAFLKFFKHSGADELQKQEEEEEEEEEEGEDSFFDIEFFSVSAGKIQSPEQRPQIPT 60

Query: 61  KLGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAGP 120
           KLG+H+NLCSDSISKRKILPIEPTSKPQSPIQ LKSAPKF  FIFKKQRSMAKNR P G 
Sbjct: 61  KLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNRPPTGQ 120

Query: 121 EQEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLKK 180
           E  E+R+L+SK TRDNSTRR  GIFPPDP   P P+P  RFSKE VQKYLKLIKPK+L+K
Sbjct: 121 EGGESRVLNSKLTRDNSTRRFGGIFPPDPCEPPVPAPTRRFSKEVVQKYLKLIKPKVLRK 180

Query: 181 PTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRLAAAVAVAPPPNR 240
           PTDSG +LSSAAVDSPVREK ENGLP GIRLVC+HLG+SKSASAA  LAA  AV P  NR
Sbjct: 181 PTDSGSDLSSAAVDSPVREKHENGLPAGIRLVCRHLGKSKSASAAGGLAA--AVIPQTNR 240

Query: 241 RDDSLLQLHDGIESAILHCKRSLNASTEKPV 271
           RDDSLLQ HDGI+SAILHCKRS NAS+EKP+
Sbjct: 241 RDDSLLQQHDGIQSAILHCKRSFNASSEKPL 269

BLAST of CmaCh15G010620 vs. ExPASy TrEMBL
Match: A0A1S3AUW4 (probable membrane-associated kinase regulator 5 OS=Cucumis melo OX=3656 GN=LOC103482925 PE=4 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 5.7e-110
Identity = 214/275 (77.82%), Postives = 235/275 (85.45%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLPKQ--EQDEEEEEEEEDSFFDLELLSVSAGKIQGPEQRPQIP 60
           MDSVA+LKFFK SGAD L KQ  E++EEEEEEEEDSFFD+E  SVSAGKIQ PEQRPQIP
Sbjct: 1   MDSVAFLKFFKHSGADELQKQEEEEEEEEEEEEEDSFFDIEFFSVSAGKIQSPEQRPQIP 60

Query: 61  TKLGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAG 120
           TKLG+H+NLCSDSISKRKILPIEPTSKPQSPIQ LKSAPKF  FIFKKQRSMAKN+AP G
Sbjct: 61  TKLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNKAPTG 120

Query: 121 PEQEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180
            E +++ + SSK TRDNSTRR  GIFP DP   P P+P  RFSKEAV+KY KLIKPK+L+
Sbjct: 121 QEGDQSGVPSSKLTRDNSTRRFGGIFPSDPCEPPVPAPTRRFSKEAVRKYFKLIKPKVLR 180

Query: 181 KPTDSGGELSS---AAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRLAAAVAVAP 240
           KPTDSG +LSS   AAVDSPVREK ENGLP GIRLVC+HLG+SKSASAA  LAA  AV P
Sbjct: 181 KPTDSGSDLSSSSAAAVDSPVREKHENGLPAGIRLVCRHLGKSKSASAAGGLAA--AVVP 240

Query: 241 PPNRRDDSLLQLHDGIESAILHCKRSLNASTEKPV 271
           PPNRRDDSLLQ HDGI+SAILHCKRS NAS+EKP+
Sbjct: 241 PPNRRDDSLLQQHDGIQSAILHCKRSFNASSEKPL 273

BLAST of CmaCh15G010620 vs. ExPASy TrEMBL
Match: A0A5D3C3T3 (Putative membrane-associated kinase regulator 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00860 PE=4 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 1.4e-108
Identity = 212/278 (76.26%), Postives = 234/278 (84.17%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLPKQ-----EQDEEEEEEEEDSFFDLELLSVSAGKIQGPEQRP 60
           MDSVA+LKFFK SGAD L KQ     E++EEEEEEEEDSFFD+E  SVSAGKIQ PEQRP
Sbjct: 1   MDSVAFLKFFKHSGADELQKQEEEEEEEEEEEEEEEEDSFFDIEFFSVSAGKIQSPEQRP 60

Query: 61  QIPTKLGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRA 120
           QIPTKLG+H+NLCSDSISKRKILPIEPTSKPQSPIQ LKSAPKF  FIFKKQRSMAKN+A
Sbjct: 61  QIPTKLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNKA 120

Query: 121 PAGPEQEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPK 180
           P   E +++ + SSK TRDNSTRR  GIFP DP   P P+P  RFSKEAV+KY KLIKPK
Sbjct: 121 PTAQEGDQSGVPSSKLTRDNSTRRFGGIFPSDPCEPPVPAPTRRFSKEAVRKYFKLIKPK 180

Query: 181 LLKKPTDSGGELSS---AAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRLAAAVA 240
           +L+KPTD+G +LSS   AAVDSPVREK ENGLP GIRLVC+HLG+SKSASAA  LAA  A
Sbjct: 181 VLRKPTDTGSDLSSSSAAAVDSPVREKHENGLPAGIRLVCRHLGKSKSASAAGGLAA--A 240

Query: 241 VAPPPNRRDDSLLQLHDGIESAILHCKRSLNASTEKPV 271
           V PPPNRRDDSLLQ HDGI+SAILHCKRS NAS+EKP+
Sbjct: 241 VVPPPNRRDDSLLQQHDGIQSAILHCKRSFNASSEKPL 276

BLAST of CmaCh15G010620 vs. NCBI nr
Match: XP_022973059.1 (probable membrane-associated kinase regulator 5 [Cucurbita maxima])

HSP 1 Score: 540.0 bits (1390), Expect = 1.2e-149
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLPKQEQDEEEEEEEEDSFFDLELLSVSAGKIQGPEQRPQIPTK 60
           MDSVAYLKFFKFSGADGLPKQEQDEEEEEEEEDSFFDLELLSVSAGKIQGPEQRPQIPTK
Sbjct: 1   MDSVAYLKFFKFSGADGLPKQEQDEEEEEEEEDSFFDLELLSVSAGKIQGPEQRPQIPTK 60

Query: 61  LGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAGPE 120
           LGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAGPE
Sbjct: 61  LGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAGPE 120

Query: 121 QEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKP 180
           QEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKP
Sbjct: 121 QEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKP 180

Query: 181 TDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRLAAAVAVAPPPNRR 240
           TDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRLAAAVAVAPPPNRR
Sbjct: 181 TDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRLAAAVAVAPPPNRR 240

Query: 241 DDSLLQLHDGIESAILHCKRSLNASTEKPVLAMDV 276
           DDSLLQLHDGIESAILHCKRSLNASTEKPVLAMDV
Sbjct: 241 DDSLLQLHDGIESAILHCKRSLNASTEKPVLAMDV 275

BLAST of CmaCh15G010620 vs. NCBI nr
Match: XP_023550940.1 (probable membrane-associated kinase regulator 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 491.1 bits (1263), Expect = 6.2e-135
Identity = 257/276 (93.12%), Postives = 260/276 (94.20%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLPKQEQ-DEEEEEEEEDSFFDLELLSVSAGKIQGPEQRPQIPT 60
           MDSVAYLKFFKFSGADGL KQEQ +EEEEEEEEDSFFDLEL SVSAGKIQ PEQRPQIPT
Sbjct: 1   MDSVAYLKFFKFSGADGLRKQEQEEEEEEEEEEDSFFDLELFSVSAGKIQSPEQRPQIPT 60

Query: 61  KLGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAGP 120
           KLGEHMNLCSDSISKRKILPIE T KPQS IQFLKSAPKFTDFIFKKQRSM+KNRAPAGP
Sbjct: 61  KLGEHMNLCSDSISKRKILPIEHTLKPQSSIQFLKSAPKFTDFIFKKQRSMSKNRAPAGP 120

Query: 121 EQEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLKK 180
           EQEETRILSSKFTRDNS RRIRGIFPPDP GVPAPSPAWRFSKEAVQKYLKLIKPKLLKK
Sbjct: 121 EQEETRILSSKFTRDNSIRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKK 180

Query: 181 PTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRLAAAVAVAPPPNR 240
           PTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAAS LA   AVAPPPNR
Sbjct: 181 PTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLA---AVAPPPNR 240

Query: 241 RDDSLLQLHDGIESAILHCKRSLNASTEKPVLAMDV 276
           RDDSLLQ HDGIESAILHCKRSLN STEKPVL +DV
Sbjct: 241 RDDSLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV 273

BLAST of CmaCh15G010620 vs. NCBI nr
Match: KAG7016845.1 (putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 491.1 bits (1263), Expect = 6.2e-135
Identity = 259/278 (93.17%), Postives = 262/278 (94.24%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLPKQEQ--DEEEEEEEEDSFFDLELLSVSAGKIQGPEQRPQIP 60
           MDSVAYLKFFKFSGADGL KQEQ  +EEEEEEEEDSFFDLELLSVSAG IQ PEQRPQIP
Sbjct: 1   MDSVAYLKFFKFSGADGLLKQEQEEEEEEEEEEEDSFFDLELLSVSAGGIQSPEQRPQIP 60

Query: 61  TKLGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAG 120
            KLGEHMNLCSDSISKRKILPIE TSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAP G
Sbjct: 61  IKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPTG 120

Query: 121 PEQEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180
            EQEETRILSSKFTRDNSTRRIRGIFPPDP GVPAPSPAWRFSKEAVQKYLKLIKPKLLK
Sbjct: 121 TEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180

Query: 181 KPTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRL-AAAVAVAPPP 240
           KPTDSGGELSSAAVDSPVREKLENGLPTGIRLV KHLGRSKSASAAS L AAAVAVAPPP
Sbjct: 181 KPTDSGGELSSAAVDSPVREKLENGLPTGIRLVRKHLGRSKSASAASGLAAAAVAVAPPP 240

Query: 241 NRRDDSLLQLHDGIESAILHCKRSLNASTEKPVLAMDV 276
           NRRDDSLL+ HDGIESAILHCKRSLN STEKPVL +DV
Sbjct: 241 NRRDDSLLEQHDGIESAILHCKRSLNGSTEKPVLGVDV 278

BLAST of CmaCh15G010620 vs. NCBI nr
Match: XP_022922154.1 (probable membrane-associated kinase regulator 5 [Cucurbita moschata])

HSP 1 Score: 491.1 bits (1263), Expect = 6.2e-135
Identity = 257/276 (93.12%), Postives = 260/276 (94.20%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLPKQEQDEEEEEEEEDSFFDLELLSVSAGKIQGPEQRPQIPTK 60
           MDSVAYLKFFKF+GADGL KQEQ EEEEEEEEDS FDLEL SVSAG IQ PEQRPQIPTK
Sbjct: 1   MDSVAYLKFFKFAGADGLLKQEQ-EEEEEEEEDSLFDLELFSVSAGGIQSPEQRPQIPTK 60

Query: 61  LGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAGPE 120
           LGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRS AKNRAPAG E
Sbjct: 61  LGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSTAKNRAPAGTE 120

Query: 121 QEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKP 180
           QEETRILSSKFTRDNSTRRIRGIFPPDP GVPAPSPAWRFSKEAVQKYLKLIKPKLLKKP
Sbjct: 121 QEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKP 180

Query: 181 TDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRL-AAAVAVAPPPNR 240
           TD+G ELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASA S L AAAVAVAPPPNR
Sbjct: 181 TDTGSELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASATSGLAAAAVAVAPPPNR 240

Query: 241 RDDSLLQLHDGIESAILHCKRSLNASTEKPVLAMDV 276
           RDDSLLQ HDGIESAILHCKRSLN STEKPVL +DV
Sbjct: 241 RDDSLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV 275

BLAST of CmaCh15G010620 vs. NCBI nr
Match: KAG6579360.1 (putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 490.7 bits (1262), Expect = 8.1e-135
Identity = 259/287 (90.24%), Postives = 262/287 (91.29%), Query Frame = 0

Query: 1   MDSVAYLKFFKFSGADGLPKQEQ-----DEEEEEEEEDSFFDLELLSVSAGKIQGPEQRP 60
           MDSVAYLKFFKFSGADGL KQEQ     +EEEEEEEEDSFFDLEL SVSAG IQ PEQRP
Sbjct: 1   MDSVAYLKFFKFSGADGLLKQEQEEEEEEEEEEEEEEDSFFDLELFSVSAGGIQSPEQRP 60

Query: 61  QIPTKLGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRA 120
           QIP KLGEHMNLCSDSISKRKILPIE TSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRA
Sbjct: 61  QIPIKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRA 120

Query: 121 PAGPEQEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPK 180
           P G EQEETRILSSKFTRDNSTRRIRGIFPPDP GVPAPSPAWRFSKEAVQKYLKLIKPK
Sbjct: 121 PTGTEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPK 180

Query: 181 LLKKPTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRL-------A 240
           LLKKPTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAAS L       A
Sbjct: 181 LLKKPTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLAAASGLAA 240

Query: 241 AAVAVAPPPNRRDDSLLQLHDGIESAILHCKRSLNASTEKPVLAMDV 276
           AAVAVAPPPNRRDDSLL+ HDGIESAILHCKRSLN STEKPVL +DV
Sbjct: 241 AAVAVAPPPNRRDDSLLEQHDGIESAILHCKRSLNGSTEKPVLGVDV 287

BLAST of CmaCh15G010620 vs. TAIR 10
Match: AT5G52870.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64080.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 94.0 bits (232), Expect = 2.0e-19
Identity = 83/264 (31.44%), Postives = 125/264 (47.35%), Query Frame = 0

Query: 13  SGADGLPKQEQDEEEEEEEEDSFFDLELLSVSAGKIQGPEQRPQIPTKLGEHMNLCSDSI 72
           S  D     E  + +  E EDSFF+L+   +S    + P+++ ++ T         S   
Sbjct: 28  SAVDSTTGSEDPDLDLCEGEDSFFELK---ISLSDFKTPKEKQRLETTTTTTTYSVS--- 87

Query: 73  SKRKILP-IEPTSKP-QSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAGPEQEETRI-LSS 132
           +K K+LP ++ +SKP QSP   LKS  KF  F FKK     K+      ++E  R  L+ 
Sbjct: 88  NKSKVLPFVDISSKPQQSPTTLLKSGQKFRAFSFKKSE---KSTTTEKKKEENNRTSLNV 147

Query: 133 KFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKPTDSGGELSS 192
           +F  D+ T                 +    F     +++  LIKP   K          +
Sbjct: 148 RFRVDDET-TTTSFRKTASIARSQQTDDTMFDDSVSKRFFSLIKPLYTKSTKKQSSSTIT 207

Query: 193 AAVDSP-VREKLENGLPTGIRLVCKHLGRSKSASAASRLAAAVAVAPPPNRRDDSLLQLH 252
           +   SP  REK  + +P+G+R V + LG+S+SAS      AA+    P NR D+SL    
Sbjct: 208 SPTSSPATREKQRSNIPSGMRSVRRQLGKSRSAS------AAIGGMSPANRIDESLQVQQ 267

Query: 253 DGIESAILHCKRSLNASTEKPVLA 273
           DGI+SAILHCK+S + S E  +L+
Sbjct: 268 DGIQSAILHCKKSFHGSRESSLLS 275

BLAST of CmaCh15G010620 vs. TAIR 10
Match: AT1G64080.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G52870.1); Has 1546 Blast hits to 738 proteins in 176 species: Archae - 0; Bacteria - 143; Metazoa - 278; Fungi - 131; Plants - 95; Viruses - 4; Other Eukaryotes - 895 (source: NCBI BLink). )

HSP 1 Score: 78.2 bits (191), Expect = 1.2e-14
Identity = 50/128 (39.06%), Postives = 68/128 (53.12%), Query Frame = 0

Query: 165 VQKYLKLIKPKLLKKPTDSGGEL--------------SSAAVDSPVREKLEN------GL 224
           +QKYLK +KP  ++     G +L              ++   DSPV  K  N       +
Sbjct: 267 MQKYLKKVKPLYIRVSRRYGEKLRHSGQLTAPSSARSTAEKADSPVATKTNNKPGNNINI 326

Query: 225 PTGIRLVCKHLGRSKSASAA----SRLAAAVAVAPPPNRRDDSLLQLHDGIESAILHCKR 269
           P G+++V KHLG+S+S+S+     S   A V       RRDDSLLQ  D I+SAILHCKR
Sbjct: 327 PAGLKVVRKHLGKSRSSSSTATTPSSTTATVTTQSESRRRDDSLLQQQDSIQSAILHCKR 386

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLX42.9e-1831.44Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 ... [more]
Q9SH581.6e-1339.06Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1IDF35.6e-150100.00probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1E2K73.0e-13593.12probable membrane-associated kinase regulator 5 OS=Cucurbita moschata OX=3662 GN... [more]
A0A0A0KK034.6e-11279.70Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139900 PE=4 SV=1[more]
A0A1S3AUW45.7e-11077.82probable membrane-associated kinase regulator 5 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5D3C3T31.4e-10876.26Putative membrane-associated kinase regulator 5 OS=Cucumis melo var. makuwa OX=1... [more]
Match NameE-valueIdentityDescription
XP_022973059.11.2e-149100.00probable membrane-associated kinase regulator 5 [Cucurbita maxima][more]
XP_023550940.16.2e-13593.12probable membrane-associated kinase regulator 5 [Cucurbita pepo subsp. pepo][more]
KAG7016845.16.2e-13593.17putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma... [more]
XP_022922154.16.2e-13593.12probable membrane-associated kinase regulator 5 [Cucurbita moschata][more]
KAG6579360.18.1e-13590.24putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma... [more]
Match NameE-valueIdentityDescription
AT5G52870.12.0e-1931.44unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G64080.11.2e-1439.06unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33929:SF4MEMBRANE-ASSOCIATED KINASE REGULATOR 5-RELATEDcoord: 1..270
IPR039619Membrane-associated kinase regulator 2/5PANTHERPTHR33929MEMBRANE-ASSOCIATED KINASE REGULATOR 2-RELATEDcoord: 1..270

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh15G010620.1CmaCh15G010620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
cellular_component GO:0005886 plasma membrane
molecular_function GO:0016301 kinase activity