CmaCh15G010020 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh15G010020
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionCDPK-related kinase 6-like isoform X2
LocationCma_Chr15: 6063913 .. 6067613 (+)
RNA-Seq ExpressionCmaCh15G010020
SyntenyCmaCh15G010020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCTCCGACGACTTTACAACCTCCATTTTCAACGCCCACCTTCTCTCCGACGACTCTTTCTTCTTCTTTCTCTTCTCAGAGGTCTCGGATTCAGAACTAAATCTCCACAGATTGCCATTTATGATAAACCATTCTCCGATTAACGTCATCCTTCCAGCTTAACTTTTCCCATGGGACACCACTGTAGTAAAAGCGTTTCTGATGTCAGTCACCACGCTGCAATTTCCGGTGGCAGCCGTCCGAGTTCTTCGCCCATACCCGAAGATACCCGGCACTCCAACTCTGTCTCTGCAACAGCGAGCCCATTTTCTAGTCCTCTGCCTTCTCGAATAGCGCCGTCGCCAGCCAGAACTAGGAGGAAATTCCGATGGCCTCTACCGCCTCCTTCGCCAGCCAAGCCCATCATGGCCATTCTACGGCAGAAATCGCGCAAGGCTTCGATACCAGAGGAAAACCAAATTGGGGAAGAGGGGTTGGGAGAGGTTCAGTTGGATAAAAGCTTTGGGTACTCGACGAACTTTGGAGCGAAGTTCGAGCTTGGGAGGGAGGTGGGGAGGGGTCATTTTGGACATACTTTCTGGGCTAAGGGTAAAAAGGGCGACCTCAAAGGAAAGCCTGTGGCCGTCAAAATCATCTCTAAAACTAAGGTTGGTTTAGTTCAATACTCATTTCTTCACCTCAATCTGTTCCTTGGTTTTGTTCATCTAAAACGTGAGATCCTAATCTAAATCTACTATAAAATCCATTTACATAAGTGGGAACGAAAGACTTACATTATTTTGATGCCAACTCGCTGCTCGGACTTATTTGCTTTGGCAGATGACATCCACCGTTGGAATCGAAGATGTTCGCCGGGAGGAAAAGATACTGAAAGCATTGTCCGGGCACAAGAATCTGGTCCATTTCCACGGAGCATTTGAAGATGCCAATAACATTTACATAGTTATGGAGTATGTGCTTGTAGATATCTGTTTTTGCCAATAGCCCTGCATTTTCTGATAGCTCAATCAAGTTAATATCAACCCTTTTTCTGAATTTGGTATCAGATTGTGTGAAGGCGGAGAACTGCTAGAAAGAATAGTGTCAAGGTAAAAATGGTTTGATTCATTTACTCTGAGAAAAACGTCGATCGACTCGGTTACAAGTTCATAACATATACCTGCAGAGGAGGAAGATATCAAGAACAAGAATCGAAAACGATCATTGTGCAGATACTCTGTGTTGTTGCATTTTGTCATCTACAAGGGATAGTGCACCGTGACTTGAAGCCAGAGGTACACATCTTTTTGTAGCTTCAATTCATTCTTTGGCTCTGAATAAAGTTCCCTATTACTTGTTGTAACTAGCTTTATCCAAATTCACTGTACCAGAATTTCCTTTTTATGAAGAAAGATGAGAACACAGGGCTCAAACTCATTGATTTTGGTCTCTCTGACTTCGTCAAGCCAGGTAAATACTTGTTGCCCTTGATAATATGTTCTTGCCTTTGTTCAATTCATTAAACATTTGCATATATTATGAAAGAATGGTGGAGAGAGATTCATGTTACCATCCAAGCTTCTGGCCTGTCCTTGAGCAGTTGCTGCTTTCAATTCCTACTTGATTTTCACTTTGCCATCTCATTAGGGGTGAGACTGTTTTCACAACGCCTATAGTTCTTCTACAGATGAACGTCTGAACGATGTCGTCGGCAGTGCATACTATGTTGCTCCTGAAGTACTCTACAGATCCTACAGTTTTGAAGCAGACATGTGGAGTATTGGTGTCATAGCATATATACTGTTGTGTGGGAGCAGACCTTTCTGGGCAAGAACTGAATCAGGAATCTTCCGCTCTGTGCTAAGAGCCGATCCTAACTTCGATGATTCCCCTTGGCCAACCATCTCTCCAGAAGCTAAAGATTTTGTGAAACGCCTTCTGAATAAAGACCATAGAAAAAGAATGACAGCAGCGCAAGCTTTAAGTAAAATCTCCAGGCTCACTTTTTCTAGTACCAATATAATGTATACAAGTATATTTGTTGATAAGCAAACAATTTCGCACCTTTGATTCACAGCACACCCGTGGTTACGAGATGAAGATCTTGCCGTGCCTTTGGATAACTTCATATACAAGTCAGTTAAGGCATATCTCCGTGCAACTCCTTTCAAGCGTGCAGCATTGAAGGTAATCTGTCACTGCAATTGAACATATCACAAATGGTGCATCAATAACTTCAACGACATCTCTTCATTATTGTAGGCACTTGCAAAAGCTTTGACAGAAGATGAACTTTTCTACCTCAGGACCCAATTCAAGCTTTTGGAGCCACAAAATGGATTTGTAACCATTGATAACTATAAAGCGGTTAGGCTCTCGCCATTCTTTTTGCTTCGACGTTTACTTTGTAATCTTTACTGTTGATGTTTACATATTATGAGTTGACCTCATGCAGGCTCTTGTGAGAAATGTAACTGATGCAATGAGAGAATCCAATGTTGCTGACATTCTGAAGATGGTGAGATCTCTTTTACTTCTTGGCCTTCAATTTAGACACTTTTGTGAGATCCCATATCAATTGGGGAGAAGAACGAAACAACTTTTATAAGAGTATGGAAACCTCTCCTTAGCAGACACGTTTGAAAACCTTGAGGGGAAGCTCGAAAGGGAAAGCCCAAAAAAACGGTGTTTCCAACAAGGATGCTAGGCCCTTAGGGGGTGGATTTGGGGGTCCCACATCGATTGGAGAAGGGAAGGAATGCTAGTAAAGACGCTGGCCCTGAAGGGGGGTGGATTGTGAGATCCCACGTCGGTTGGGGAGGAGAACGAAACACCATTTATAAGGGTGTGGAGACCTCTCCCTAGCAGATGCATTTTCAAGCCTTGAAGGGAAGTTCAAAAATGATAATATCTGCTAGCGGTGGGCTTGGGTTGTTACACCTTTTATGCATATACTAAAATCAAGTTACATTCTCTGCAACTCTGGCATAATGCTCTCCGGCCGTGGTTGATATTCTTTTTCACTAACCTTTCCAACTTGTATTTAGATGGAACCTCTCGCTAACAAAAGAATGGGCTTTGAGGAGTTCTGCGCAGCTGCAATCAGTGTATATCAGCTGGAGGCTGTTGCAGGATGGGAAAGCATCGCCACCCGAGCTTTCGAGTATTTCGAGCAGGAAGGAAATCGAGTCATTTCCGTACATGAGTTAGTTCAGGTAAGAAACAATTCGACGCTTGGAATTACTACATTTTACACAACTAACAACAGTGAATTGAAGTGCAAGTTTAAAATGTGTTCAGGAAATGAATCTGGGTCCTATAGCTCATTCTTTTCTCCAGAATTGGACAAGAAGTTCTGATGGGAAACTCAGTTTTTTCGGATATACAAAATTTCTGCATGGTGTTACAATCCGCTACTCTAATACAAGACATTAACACACAGAAGAAGCAGATAGTTCAATAGCTGTACACTTGATTCATTTTTTCTCCTCATTGTATTATCAATTTGATTCCTAGCAGCTTCAAAACACTGAATAAAAGAGGGCCATGCGATTGAGAAACATAAAAAGAAATGCGGCTGTACCATGTATTGCATTCTTTCCCATTTCACCGCCCAGTTCTTTGTAATCCTTCCTACATGTAAAAACAAGGCAGAGATCAGAATACAGATTTTACTGAGACTTGCCAAACTGATATGCTATATTATGTTCTTTCATTCTCCC

mRNA sequence

TCCTCCGACGACTTTACAACCTCCATTTTCAACGCCCACCTTCTCTCCGACGACTCTTTCTTCTTCTTTCTCTTCTCAGAGGTCTCGGATTCAGAACTAAATCTCCACAGATTGCCATTTATGATAAACCATTCTCCGATTAACGTCATCCTTCCAGCTTAACTTTTCCCATGGGACACCACTGTAGTAAAAGCGTTTCTGATGTCAGTCACCACGCTGCAATTTCCGGTGGCAGCCGTCCGAGTTCTTCGCCCATACCCGAAGATACCCGGCACTCCAACTCTGTCTCTGCAACAGCGAGCCCATTTTCTAGTCCTCTGCCTTCTCGAATAGCGCCGTCGCCAGCCAGAACTAGGAGGAAATTCCGATGGCCTCTACCGCCTCCTTCGCCAGCCAAGCCCATCATGGCCATTCTACGGCAGAAATCGCGCAAGGCTTCGATACCAGAGGAAAACCAAATTGGGGAAGAGGGGTTGGGAGAGGTTCAGTTGGATAAAAGCTTTGGGTACTCGACGAACTTTGGAGCGAAGTTCGAGCTTGGGAGGGAGGTGGGGAGGGGTCATTTTGGACATACTTTCTGGGCTAAGGGTAAAAAGGGCGACCTCAAAGGAAAGCCTGTGGCCGTCAAAATCATCTCTAAAACTAAGATGACATCCACCGTTGGAATCGAAGATGTTCGCCGGGAGGAAAAGATACTGAAAGCATTGTCCGGGCACAAGAATCTGGTCCATTTCCACGGAGCATTTGAAGATGCCAATAACATTTACATAGTTATGGAATTGTGTGAAGGCGGAGAACTGCTAGAAAGAATAGTGTCAAGAGGAGGAAGATATCAAGAACAAGAATCGAAAACGATCATTGTGCAGATACTCTGTGTTGTTGCATTTTGTCATCTACAAGGGATAGTGCACCGTGACTTGAAGCCAGAGAATTTCCTTTTTATGAAGAAAGATGAGAACACAGGGCTCAAACTCATTGATTTTGGTCTCTCTGACTTCGTCAAGCCAGATGAACGTCTGAACGATGTCGTCGGCAGTGCATACTATGTTGCTCCTGAAGTACTCTACAGATCCTACAGTTTTGAAGCAGACATGTGGAGTATTGGTGTCATAGCATATATACTGTTGTGTGGGAGCAGACCTTTCTGGGCAAGAACTGAATCAGGAATCTTCCGCTCTGTGCTAAGAGCCGATCCTAACTTCGATGATTCCCCTTGGCCAACCATCTCTCCAGAAGCTAAAGATTTTGTGAAACGCCTTCTGAATAAAGACCATAGAAAAAGAATGACAGCAGCGCAAGCTTTAACACACCCGTGGTTACGAGATGAAGATCTTGCCGTGCCTTTGGATAACTTCATATACAAGTCAGTTAAGGCATATCTCCGTGCAACTCCTTTCAAGCGTGCAGCATTGAAGGCACTTGCAAAAGCTTTGACAGAAGATGAACTTTTCTACCTCAGGACCCAATTCAAGCTTTTGGAGCCACAAAATGGATTTGTAACCATTGATAACTATAAAGCGATGGAACCTCTCGCTAACAAAAGAATGGGCTTTGAGGAGTTCTGCGCAGCTGCAATCAGTGTATATCAGCTGGAGGCTGTTGCAGGATGGGAAAGCATCGCCACCCGAGCTTTCGAGTATTTCGAGCAGGAAGGAAATCGAGTCATTTCCGTACATGAGTTAGTTCAGGAAATGAATCTGGGTCCTATAGCTCATTCTTTTCTCCAGAATTGGACAAGAAGTTCTGATGGGAAACTCAGTTTTTTCGGATATACAAAATTTCTGCATGGTGTTACAATCCGCTACTCTAATACAAGACATTAACACACAGAAGAAGCAGATAGTTCAATAGCTGTACACTTGATTCATTTTTTCTCCTCATTGTATTATCAATTTGATTCCTAGCAGCTTCAAAACACTGAATAAAAGAGGGCCATGCGATTGAGAAACATAAAAAGAAATGCGGCTGTACCATGTATTGCATTCTTTCCCATTTCACCGCCCAGTTCTTTGTAATCCTTCCTACATGTAAAAACAAGGCAGAGATCAGAATACAGATTTTACTGAGACTTGCCAAACTGATATGCTATATTATGTTCTTTCATTCTCCC

Coding sequence (CDS)

ATGGGACACCACTGTAGTAAAAGCGTTTCTGATGTCAGTCACCACGCTGCAATTTCCGGTGGCAGCCGTCCGAGTTCTTCGCCCATACCCGAAGATACCCGGCACTCCAACTCTGTCTCTGCAACAGCGAGCCCATTTTCTAGTCCTCTGCCTTCTCGAATAGCGCCGTCGCCAGCCAGAACTAGGAGGAAATTCCGATGGCCTCTACCGCCTCCTTCGCCAGCCAAGCCCATCATGGCCATTCTACGGCAGAAATCGCGCAAGGCTTCGATACCAGAGGAAAACCAAATTGGGGAAGAGGGGTTGGGAGAGGTTCAGTTGGATAAAAGCTTTGGGTACTCGACGAACTTTGGAGCGAAGTTCGAGCTTGGGAGGGAGGTGGGGAGGGGTCATTTTGGACATACTTTCTGGGCTAAGGGTAAAAAGGGCGACCTCAAAGGAAAGCCTGTGGCCGTCAAAATCATCTCTAAAACTAAGATGACATCCACCGTTGGAATCGAAGATGTTCGCCGGGAGGAAAAGATACTGAAAGCATTGTCCGGGCACAAGAATCTGGTCCATTTCCACGGAGCATTTGAAGATGCCAATAACATTTACATAGTTATGGAATTGTGTGAAGGCGGAGAACTGCTAGAAAGAATAGTGTCAAGAGGAGGAAGATATCAAGAACAAGAATCGAAAACGATCATTGTGCAGATACTCTGTGTTGTTGCATTTTGTCATCTACAAGGGATAGTGCACCGTGACTTGAAGCCAGAGAATTTCCTTTTTATGAAGAAAGATGAGAACACAGGGCTCAAACTCATTGATTTTGGTCTCTCTGACTTCGTCAAGCCAGATGAACGTCTGAACGATGTCGTCGGCAGTGCATACTATGTTGCTCCTGAAGTACTCTACAGATCCTACAGTTTTGAAGCAGACATGTGGAGTATTGGTGTCATAGCATATATACTGTTGTGTGGGAGCAGACCTTTCTGGGCAAGAACTGAATCAGGAATCTTCCGCTCTGTGCTAAGAGCCGATCCTAACTTCGATGATTCCCCTTGGCCAACCATCTCTCCAGAAGCTAAAGATTTTGTGAAACGCCTTCTGAATAAAGACCATAGAAAAAGAATGACAGCAGCGCAAGCTTTAACACACCCGTGGTTACGAGATGAAGATCTTGCCGTGCCTTTGGATAACTTCATATACAAGTCAGTTAAGGCATATCTCCGTGCAACTCCTTTCAAGCGTGCAGCATTGAAGGCACTTGCAAAAGCTTTGACAGAAGATGAACTTTTCTACCTCAGGACCCAATTCAAGCTTTTGGAGCCACAAAATGGATTTGTAACCATTGATAACTATAAAGCGATGGAACCTCTCGCTAACAAAAGAATGGGCTTTGAGGAGTTCTGCGCAGCTGCAATCAGTGTATATCAGCTGGAGGCTGTTGCAGGATGGGAAAGCATCGCCACCCGAGCTTTCGAGTATTTCGAGCAGGAAGGAAATCGAGTCATTTCCGTACATGAGTTAGTTCAGGAAATGAATCTGGGTCCTATAGCTCATTCTTTTCTCCAGAATTGGACAAGAAGTTCTGATGGGAAACTCAGTTTTTTCGGATATACAAAATTTCTGCATGGTGTTACAATCCGCTACTCTAATACAAGACATTAA

Protein sequence

MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPARTRRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAKFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVTIDNYKAMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTKFLHGVTIRYSNTRH
Homology
BLAST of CmaCh15G010020 vs. ExPASy Swiss-Prot
Match: Q9SG12 (CDPK-related kinase 6 OS=Arabidopsis thaliana OX=3702 GN=CRK6 PE=1 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 2.1e-198
Identity = 359/592 (60.64%), Postives = 445/592 (75.17%), Query Frame = 0

Query: 1   MGHHCSKSVSDVS-------------HHAAISGGSRPSSSPIPEDTRHSNSVSATASPFS 60
           MGH  S+++S V              H +  +     SSS IP+    S       SPF 
Sbjct: 1   MGHCYSRNISTVDDDDEIPSATAQLPHRSHQNHHQTSSSSSIPQSPATSEVNPYNISPFQ 60

Query: 61  SPLPSRIAPSPART-RRKFRWPLPPPSPAKPIMAILRQK------SRKASIPEENQIGEE 120
           SPLP+ +APSPART  RKF+WP PPPSPAKPIMA LR++       R   IPE+++ G  
Sbjct: 61  SPLPAGVAPSPARTPGRKFKWPFPPPSPAKPIMAALRRRRGTAPHPRDGPIPEDSEAGGS 120

Query: 121 --GLGEVQLDKSFGYSTNFGAKFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKT 180
             G+GE +LDK+FG++ NF  K+ELGREVGRGHFGHT WAK KKG +KG+ VAVKIISK+
Sbjct: 121 GGGIGE-RLDKNFGFAKNFEGKYELGREVGRGHFGHTCWAKAKKGKIKGQTVAVKIISKS 180

Query: 181 KMTSTVGIEDVRREEKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRG 240
           KMTS + IEDVRRE K+LKALSGH ++V F+  FED++N+++VMELCEGGELL+ I++RG
Sbjct: 181 KMTSALSIEDVRREVKLLKALSGHSHMVKFYDVFEDSDNVFVVMELCEGGELLDSILARG 240

Query: 241 GRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVK 300
           GRY E E+K I+VQIL   AF HLQG+VHRDLKPENFLF  K+E+  LK+IDFGLSD+ +
Sbjct: 241 GRYPEAEAKRILVQILSATAFFHLQGVVHRDLKPENFLFTSKNEDAVLKVIDFGLSDYAR 300

Query: 301 PDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVL 360
            D+RLNDVVGSAYYVAPEVL+RSYS EAD+WSIGVI+YILLCGSRPF+ RTES IFR VL
Sbjct: 301 FDQRLNDVVGSAYYVAPEVLHRSYSTEADIWSIGVISYILLCGSRPFYGRTESAIFRCVL 360

Query: 361 RADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYK 420
           RA+PNFDD PWP+ISP AKDFVKRLLNKDHRKRMTAAQAL HPWLRDE+  + LD  IYK
Sbjct: 361 RANPNFDDLPWPSISPIAKDFVKRLLNKDHRKRMTAAQALAHPWLRDENPGLLLDFSIYK 420

Query: 421 SVKAYLRATPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVTIDNY---------- 480
            VK+Y+RA+PF+RAALK+L+KA+ E+EL +L+ QF LLEP++G + + N+          
Sbjct: 421 LVKSYIRASPFRRAALKSLSKAIPEEELVFLKAQFMLLEPEDGGLHLHNFTTALTRYATD 480

Query: 481 -----------KAMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNR 540
                        M+PLA+K++ FEEFCAA++SVYQLEA+  WE IAT AFE+FE EG+R
Sbjct: 481 AMIESRLPDILNMMQPLAHKKLDFEEFCAASVSVYQLEALEEWEQIATVAFEHFESEGSR 540

Query: 541 VISVHELVQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTKFLHGVTIRYSNTR 550
            ISV EL +EM+LGP A+  L++W RS DGKL+F GY KFLHGVT+R S++R
Sbjct: 541 AISVQELAEEMSLGPNAYPLLKDWIRSLDGKLNFLGYAKFLHGVTVRSSSSR 591

BLAST of CmaCh15G010020 vs. ExPASy Swiss-Prot
Match: Q9FIM9 (CDPK-related kinase 4 OS=Arabidopsis thaliana OX=3702 GN=CRK4 PE=1 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 3.7e-198
Identity = 358/593 (60.37%), Postives = 447/593 (75.38%), Query Frame = 0

Query: 1   MGHHCSKSVSDVSHHAAISGGSRPSSSP--------IPEDTRHSNSV---SATASPFSSP 60
           MGH  S+++S V      +G    S+ P        IP+    S +    S   SPF SP
Sbjct: 1   MGHCYSRNISAVEDDEIPTGNGEVSNQPSQNHRHASIPQSPVASGTPEVNSYNISPFQSP 60

Query: 61  LPSRIAPSPART-RRKFRWPLPPPSPAKPIMAILRQK------SRKASIPEENQI----- 120
           LP+ +APSPART  RKF+WP PPPSPAKPIMA LR++       R   IPE+++      
Sbjct: 61  LPAGVAPSPARTPGRKFKWPFPPPSPAKPIMAALRRRRGAPPQPRDEPIPEDSEDVVDHG 120

Query: 121 GEEGLGEVQLDKSFGYSTNFGAKFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISK 180
           G+ G GE +LDK+FG+  NF  K+ELG+EVGRGHFGHT WAK KKG +K + VAVKIISK
Sbjct: 121 GDSGGGE-RLDKNFGFGKNFEGKYELGKEVGRGHFGHTCWAKAKKGKMKNQTVAVKIISK 180

Query: 181 TKMTSTVGIEDVRREEKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSR 240
            KMTST+ IEDVRRE K+LKALSGH+++V F+  +EDA+N+++VMELCEGGELL+RI++R
Sbjct: 181 AKMTSTLSIEDVRREVKLLKALSGHRHMVKFYDVYEDADNVFVVMELCEGGELLDRILAR 240

Query: 241 GGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFV 300
           GGRY E ++K I+VQIL   AF HLQG+VHRDLKPENFLF  ++E+  LK+IDFGLSDF+
Sbjct: 241 GGRYPEVDAKRILVQILSATAFFHLQGVVHRDLKPENFLFTSRNEDAILKVIDFGLSDFI 300

Query: 301 KPDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSV 360
           + D+RLNDVVGSAYYVAPEVL+RSYS EADMWSIGVI+YILLCGSRPF+ RTES IFR V
Sbjct: 301 RYDQRLNDVVGSAYYVAPEVLHRSYSTEADMWSIGVISYILLCGSRPFYGRTESAIFRCV 360

Query: 361 LRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIY 420
           LRA+PNF+D PWP+ISP AKDFVKRLLNKDHRKRMTAAQAL HPWLRDE+  + LD  +Y
Sbjct: 361 LRANPNFEDMPWPSISPTAKDFVKRLLNKDHRKRMTAAQALAHPWLRDENPGLLLDFSVY 420

Query: 421 KSVKAYLRATPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVTI------------ 480
           K VK+Y+RA+PF+R+ALKAL+KA+ ++EL +L+ QF LL+P++G +++            
Sbjct: 421 KLVKSYIRASPFRRSALKALSKAIPDEELVFLKAQFMLLDPKDGGLSLNCFTMALTRYAT 480

Query: 481 ---------DNYKAMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGN 540
                    D    M+PLA K++ FEEFCAAA+SVYQLEA+  WE IAT AFE+FE EGN
Sbjct: 481 DAMMESRLPDILNTMQPLAQKKLDFEEFCAAAVSVYQLEALEEWEQIATSAFEHFEHEGN 540

Query: 541 RVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTKFLHGVTIRYSNTR 550
           R+ISV EL  EM++GP A+  L++W RSSDGKLSF GY KFLHGVT+R S++R
Sbjct: 541 RIISVQELAGEMSVGPSAYPLLKDWIRSSDGKLSFLGYAKFLHGVTVRSSSSR 592

BLAST of CmaCh15G010020 vs. ExPASy Swiss-Prot
Match: Q9ZUZ2 (CDPK-related kinase 3 OS=Arabidopsis thaliana OX=3702 GN=CRK3 PE=1 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 1.5e-196
Identity = 352/547 (64.35%), Postives = 425/547 (77.70%), Query Frame = 0

Query: 36  SNSVSATASPF----SSPLPSRIAPSPART---RRKFRWPLPPPSPAKPIMAILRQ---- 95
           S++ S   SPF    +SPLPS ++PSPART   RR FR P PPPSPAK I A L +    
Sbjct: 51  SSNPSPWPSPFPHGSASPLPSGVSPSPARTSTPRRFFRRPFPPPSPAKHIKASLIKRLGV 110

Query: 96  KSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAKFELGREVGRGHFGHTFWAKGKKGD 155
           K ++  IPEE   G E   E  LDKSFGY  NFGAK+ELG+EVGRGHFGHT   +GKKGD
Sbjct: 111 KPKEGPIPEER--GTE--PEQSLDKSFGYGKNFGAKYELGKEVGRGHFGHTCSGRGKKGD 170

Query: 156 LKGKPVAVKIISKTKMTSTVGIEDVRREEKILKALSGHKNLVHFHGAFEDANNIYIVMEL 215
           +K  P+AVKIISK KMT+ + IEDVRRE K+LK+LSGHK L+ ++ A EDANN+YIVMEL
Sbjct: 171 IKDHPIAVKIISKAKMTTAIAIEDVRREVKLLKSLSGHKYLIKYYDACEDANNVYIVMEL 230

Query: 216 CEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPENFLFMKKDENT 275
           C+GGELL+RI++RGG+Y E ++K I+VQIL VV+FCHLQG+VHRDLKPENFLF    E++
Sbjct: 231 CDGGELLDRILARGGKYPEDDAKAIVVQILTVVSFCHLQGVVHRDLKPENFLFTSSREDS 290

Query: 276 GLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRP 335
            LKLIDFGLSDF++PDERLND+VGSAYYVAPEVL+RSYS EAD+WSIGVI YILLCGSRP
Sbjct: 291 DLKLIDFGLSDFIRPDERLNDIVGSAYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRP 350

Query: 336 FWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLR 395
           FWARTESGIFR+VLR +PN+DD PWP+ S E KDFVKRLLNKD+RKRM+A QALTHPWLR
Sbjct: 351 FWARTESGIFRTVLRTEPNYDDVPWPSCSSEGKDFVKRLLNKDYRKRMSAVQALTHPWLR 410

Query: 396 DEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDELFYLRTQFKLLEP-QNGFV 455
           D+   +PLD  IYK VKAYL ATP +RAALKALAKALTE+EL YLR QF LL P ++G V
Sbjct: 411 DDSRVIPLDILIYKLVKAYLHATPLRRAALKALAKALTENELVYLRAQFMLLGPNKDGSV 470

Query: 456 TIDNYK---------------------AMEPLANKRMGFEEFCAAAISVYQLEAVAGWES 515
           +++N+K                      ME LA ++M FEEFCAAAIS++QLEAV  WE 
Sbjct: 471 SLENFKTALMQNATDAMRESRVPEILHTMESLAYRKMYFEEFCAAAISIHQLEAVDAWEE 530

Query: 516 IATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTKFLHGVT 550
           IAT  F++FE EGNRVI++ EL +E+N+G  A+  L++W RSSDGKLS+ G+TKFLHGVT
Sbjct: 531 IATAGFQHFETEGNRVITIEELARELNVGASAYGHLRDWVRSSDGKLSYLGFTKFLHGVT 590

BLAST of CmaCh15G010020 vs. ExPASy Swiss-Prot
Match: Q9SCS2 (CDPK-related kinase 5 OS=Arabidopsis thaliana OX=3702 GN=CRK5 PE=2 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 3.6e-177
Identity = 321/543 (59.12%), Postives = 404/543 (74.40%), Query Frame = 0

Query: 45  PFSSPLPSRI-------APSPART------RRKFRWPLPPPSPAKPIMAILRQ-----KS 104
           PF SP P+         A SPA        +R F+ P PPPSPAK I A+L +     K 
Sbjct: 57  PFYSPSPAHYFFSKKTPARSPATNSTNSTPKRFFKRPFPPPSPAKHIRAVLARRHGSVKP 116

Query: 105 RKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAKFELGREVGRGHFGHTFWAKGKKGDLK 164
             ++IPE ++    G+G   LDKSFG+S +F +K+ELG EVGRGHFG+T  AK KKGD K
Sbjct: 117 NSSAIPEGSEAEGGGVG---LDKSFGFSKSFASKYELGDEVGRGHFGYTCAAKFKKGDNK 176

Query: 165 GKPVAVKIISKTKMTSTVGIEDVRREEKILKALSGHKNLVHFHGAFEDANNIYIVMELCE 224
           G+ VAVK+I K KMT+ + IEDVRRE KIL+ALSGH NL HF+ A+ED +N+YIVMELCE
Sbjct: 177 GQQVAVKVIPKAKMTTAIAIEDVRREVKILRALSGHNNLPHFYDAYEDHDNVYIVMELCE 236

Query: 225 GGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPENFLFMKKDENTGL 284
           GGELL+RI+SRGG+Y E+++KT+++QIL VVAFCHLQG+VHRDLKPENFLF  K++ + L
Sbjct: 237 GGELLDRILSRGGKYTEEDAKTVMIQILNVVAFCHLQGVVHRDLKPENFLFTSKEDTSQL 296

Query: 285 KLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFW 344
           K IDFGLSD+V+PDERLND+VGSAYYVAPEVL+RSYS EAD+WS+GVI YILLCGSRPFW
Sbjct: 297 KAIDFGLSDYVRPDERLNDIVGSAYYVAPEVLHRSYSTEADIWSVGVIVYILLCGSRPFW 356

Query: 345 ARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRD- 404
           ARTESGIFR+VL+ADP+FDD PWP +S EA+DFVKRLLNKD RKR+TAAQAL+HPW++D 
Sbjct: 357 ARTESGIFRAVLKADPSFDDPPWPLLSSEARDFVKRLLNKDPRKRLTAAQALSHPWIKDS 416

Query: 405 EDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDELFYLRTQFKLLEP-QNGFVT 464
            D  VP+D  ++K ++AYLR++  ++AAL+AL+K LT DELFYLR QF LLEP +NG ++
Sbjct: 417 NDAKVPMDILVFKLMRAYLRSSSLRKAALRALSKTLTVDELFYLREQFALLEPSKNGTIS 476

Query: 465 IDNYKA---------------------MEPLANKRMGFEEFCAAAISVYQLEAVAGWESI 524
           ++N K+                     +  L  +RM FEEFCAAA+SV+QLEA+  WE  
Sbjct: 477 LENIKSALMKMATDAMKDSRIPEFLGQLSALQYRRMDFEEFCAAALSVHQLEALDRWEQH 536

Query: 525 ATRAFEYFEQEGNRVISVHELVQEMNLGPI--AHSFLQNWTRSSDGKLSFFGYTKFLHGV 545
           A  A+E FE+EGNR I + EL  E+ LGP    H+ L +W R +DGKLSF G+ K LHGV
Sbjct: 537 ARCAYELFEKEGNRPIMIDELASELGLGPSVPVHAVLHDWLRHTDGKLSFLGFVKLLHGV 596

BLAST of CmaCh15G010020 vs. ExPASy Swiss-Prot
Match: Q10KY3 (Calcium/calmodulin-dependent serine/threonine-protein kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CAMK1 PE=1 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 3.1e-173
Identity = 306/522 (58.62%), Postives = 390/522 (74.71%), Query Frame = 0

Query: 48  SPLPSRIAPSPARTRRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQL 107
           SP  S +A +PA  R  F+ P PPPSPAK I A+L ++      P E  I E G   V L
Sbjct: 77  SPANSSVASTPA--RGGFKRPFPPPSPAKHIRALLARRHGSVK-PNEASIPESGEPGVAL 136

Query: 108 DKSFGYSTNFGAKFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIE 167
           DK FG+S +F AK+ELGREVGRGHFG+T  A  KKG+LKG  VAVK+I K KMT+ + IE
Sbjct: 137 DKGFGFSRHFAAKYELGREVGRGHFGYTCAATCKKGELKGDDVAVKVIPKAKMTTAIAIE 196

Query: 168 DVRREEKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESK 227
           DVRRE +IL +L+GH NLV F+ A+ED  N+YIVMELC+GGELL+RI++RGG+Y E+++K
Sbjct: 197 DVRREVRILSSLAGHSNLVQFYDAYEDEENVYIVMELCKGGELLDRILARGGKYSEEDAK 256

Query: 228 TIIVQILCVVAFCHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVV 287
            ++ QIL V +FCHLQG+VHRDLKPENFLF  KDEN+ +K+IDFGLSDFVKPDERLND+V
Sbjct: 257 VVMRQILSVASFCHLQGVVHRDLKPENFLFSSKDENSAMKVIDFGLSDFVKPDERLNDIV 316

Query: 288 GSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDS 347
           GSAYYVAPEVL+RSY  EADMWSIGVI YILLCGSRPFWARTESGIFR+VL+ADP+F+++
Sbjct: 317 GSAYYVAPEVLHRSYGTEADMWSIGVIVYILLCGSRPFWARTESGIFRAVLKADPSFEEA 376

Query: 348 PWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLR-DEDLAVPLDNFIYKSVKAYLRA 407
           PWPT+S EAKDFV+RLLNKD+RKRMTAAQAL HPW+R  E++ +PLD  IY+ ++AY+ +
Sbjct: 377 PWPTLSAEAKDFVRRLLNKDYRKRMTAAQALCHPWIRGTEEVKLPLDMIIYRLMRAYISS 436

Query: 408 TPFKRAALKALAKALTEDELFYLRTQFKLLEP-QNGFVTIDNYK---------------- 467
           +  +RAAL+ALAK LT D+++YLR QF+L+ P ++  +T+ N K                
Sbjct: 437 SSLRRAALRALAKTLTTDQIYYLREQFELIGPNKSDLITLQNLKTALMKNSTNAMKDSRV 496

Query: 468 -----AMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHEL 527
                 +  +  +++ FEEF AAAISVYQ+E +  WE  A +A+E+F++EGNR I + EL
Sbjct: 497 VDFVNTISNIQYRKLDFEEFSAAAISVYQMEGLETWEQHARQAYEFFDKEGNRPIVIDEL 556

Query: 528 VQEMNLGPIA--HSFLQNWTRSSDGKLSFFGYTKFLHGVTIR 545
             E+ LGP    H  LQ+W R  DGKLSF G+ K LHGV+ R
Sbjct: 557 ASELGLGPSVPLHVVLQDWIRHPDGKLSFLGFMKLLHGVSSR 595

BLAST of CmaCh15G010020 vs. ExPASy TrEMBL
Match: A0A6J1IDP0 (CDPK-related kinase 6-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111472964 PE=4 SV=1)

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 550/571 (96.32%), Postives = 550/571 (96.32%), Query Frame = 0

Query: 1   MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60
           MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR
Sbjct: 1   MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60

Query: 61  TRRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAK 120
           TRRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAK
Sbjct: 61  TRRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAK 120

Query: 121 FELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKALS 180
           FELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKALS
Sbjct: 121 FELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKALS 180

Query: 181 GHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFC 240
           GHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFC
Sbjct: 181 GHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFC 240

Query: 241 HLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYR 300
           HLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYR
Sbjct: 241 HLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYR 300

Query: 301 SYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFV 360
           SYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFV
Sbjct: 301 SYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFV 360

Query: 361 KRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKA 420
           KRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKA
Sbjct: 361 KRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKA 420

Query: 421 LTEDELFYLRTQFKLLEPQNGFVTIDNYKA---------------------MEPLANKRM 480
           LTEDELFYLRTQFKLLEPQNGFVTIDNYKA                     MEPLANKRM
Sbjct: 421 LTEDELFYLRTQFKLLEPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRM 480

Query: 481 GFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQ 540
           GFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQ
Sbjct: 481 GFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQ 540

Query: 541 NWTRSSDGKLSFFGYTKFLHGVTIRYSNTRH 551
           NWTRSSDGKLSFFGYTKFLHGVTIRYSNTRH
Sbjct: 541 NWTRSSDGKLSFFGYTKFLHGVTIRYSNTRH 571

BLAST of CmaCh15G010020 vs. ExPASy TrEMBL
Match: A0A6J1E874 (CDPK-related kinase 4 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430287 PE=4 SV=1)

HSP 1 Score: 1055.4 bits (2728), Expect = 7.9e-305
Identity = 534/572 (93.36%), Postives = 541/572 (94.58%), Query Frame = 0

Query: 1   MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60
           MGHHCSKSVSD+S+HA ISGGS P+     EDTRHSNSVSATASPFSSPLPS IAPSPAR
Sbjct: 1   MGHHCSKSVSDISNHAVISGGSHPT-----EDTRHSNSVSATASPFSSPLPSGIAPSPAR 60

Query: 61  -TRRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGA 120
            TRRKFRWPLPPPSPAKPIMAILR+KSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGA
Sbjct: 61  TTRRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGA 120

Query: 121 KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKAL 180
           KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISK+KMTSTVGIEDVRRE KILKAL
Sbjct: 121 KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKAL 180

Query: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240
           SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF
Sbjct: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240

Query: 241 CHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLY 300
           CHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLY
Sbjct: 241 CHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLY 300

Query: 301 RSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDF 360
           RSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDF
Sbjct: 301 RSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDF 360

Query: 361 VKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAK 420
           VKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAK
Sbjct: 361 VKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAK 420

Query: 421 ALTEDELFYLRTQFKLLEPQNGFVTIDNYKA---------------------MEPLANKR 480
           ALTEDELFYLRTQFKLL+PQNGFVTIDNYKA                     MEPLANKR
Sbjct: 421 ALTEDELFYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKR 480

Query: 481 MGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFL 540
           MGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFL
Sbjct: 481 MGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFL 540

Query: 541 QNWTRSSDGKLSFFGYTKFLHGVTIRYSNTRH 551
           QNWTRSSDGKLSFFGYT+FLHGVTIRYSNTRH
Sbjct: 541 QNWTRSSDGKLSFFGYTRFLHGVTIRYSNTRH 567

BLAST of CmaCh15G010020 vs. ExPASy TrEMBL
Match: A0A6J1IHA7 (CDPK-related kinase 6-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111472964 PE=4 SV=1)

HSP 1 Score: 1001.5 bits (2588), Expect = 1.4e-288
Identity = 507/530 (95.66%), Postives = 507/530 (95.66%), Query Frame = 0

Query: 1   MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60
           MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR
Sbjct: 1   MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60

Query: 61  TRRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAK 120
           TRRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAK
Sbjct: 61  TRRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAK 120

Query: 121 FELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKALS 180
           FELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKALS
Sbjct: 121 FELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKALS 180

Query: 181 GHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFC 240
           GHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFC
Sbjct: 181 GHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFC 240

Query: 241 HLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYR 300
           HLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYR
Sbjct: 241 HLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYR 300

Query: 301 SYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFV 360
           SYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFV
Sbjct: 301 SYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFV 360

Query: 361 KRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKA 420
           KRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKA
Sbjct: 361 KRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKA 420

Query: 421 LTEDELFYLRTQFKLLEPQNGFVTIDNYKA---------------------MEPLANKRM 480
           LTEDELFYLRTQFKLLEPQNGFVTIDNYKA                     MEPLANKRM
Sbjct: 421 LTEDELFYLRTQFKLLEPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRM 480

Query: 481 GFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMN 510
           GFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQ  N
Sbjct: 481 GFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQVRN 530

BLAST of CmaCh15G010020 vs. ExPASy TrEMBL
Match: A0A6J1E2V0 (CDPK-related kinase 4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430287 PE=4 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 1.8e-277
Identity = 492/531 (92.66%), Postives = 498/531 (93.79%), Query Frame = 0

Query: 1   MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60
           MGHHCSKSVSD+S+HA ISGGS P+     EDTRHSNSVSATASPFSSPLPS IAPSPAR
Sbjct: 1   MGHHCSKSVSDISNHAVISGGSHPT-----EDTRHSNSVSATASPFSSPLPSGIAPSPAR 60

Query: 61  -TRRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGA 120
            TRRKFRWPLPPPSPAKPIMAILR+KSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGA
Sbjct: 61  TTRRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGA 120

Query: 121 KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKAL 180
           KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISK+KMTSTVGIEDVRRE KILKAL
Sbjct: 121 KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKAL 180

Query: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240
           SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF
Sbjct: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240

Query: 241 CHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLY 300
           CHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLY
Sbjct: 241 CHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLY 300

Query: 301 RSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDF 360
           RSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDF
Sbjct: 301 RSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDF 360

Query: 361 VKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAK 420
           VKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAK
Sbjct: 361 VKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAK 420

Query: 421 ALTEDELFYLRTQFKLLEPQNGFVTIDNYKA---------------------MEPLANKR 480
           ALTEDELFYLRTQFKLL+PQNGFVTIDNYKA                     MEPLANKR
Sbjct: 421 ALTEDELFYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKR 480

Query: 481 MGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMN 510
           MGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQ  N
Sbjct: 481 MGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQVRN 526

BLAST of CmaCh15G010020 vs. ExPASy TrEMBL
Match: A0A6J1K6B2 (CDPK-related kinase 6-like OS=Cucurbita maxima OX=3661 GN=LOC111491067 PE=4 SV=1)

HSP 1 Score: 949.9 bits (2454), Expect = 4.7e-273
Identity = 486/585 (83.08%), Postives = 513/585 (87.69%), Query Frame = 0

Query: 1   MGHHCSKSVSDVSHHAAISGGSRPSS-----SPI--------PEDTRHSNSVSATASPFS 60
           MGH CSKSVSDV+H   ISGGS   S     SPI        PE+ R S SVSAT SPFS
Sbjct: 1   MGHLCSKSVSDVNHDTGISGGSHTRSSHSGASPIAYGDVYSRPEEFRQSYSVSATGSPFS 60

Query: 61  SPLPSRIAPSPART-RRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQ 120
           SPLP+ IAPSPART  RKFRWPLPPPSPAKPIMAILR+KSRKASIPEEN+IGEEG+GE+Q
Sbjct: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENRIGEEGVGEIQ 120

Query: 121 LDKSFGYSTNFGAKFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGI 180
           LDKSFGYS NFGAKFELG+E+GRGHFGHTFWA+GKKGDLKGKPVAVKIISK+KMTS VGI
Sbjct: 121 LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWARGKKGDLKGKPVAVKIISKSKMTSAVGI 180

Query: 181 EDVRREEKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQES 240
           EDVRRE KILKALSGHKNLV FH AFEDANNIY+VMELCEGGELLERIVSRGGRY EQES
Sbjct: 181 EDVRREVKILKALSGHKNLVQFHDAFEDANNIYMVMELCEGGELLERIVSRGGRYPEQES 240

Query: 241 KTIIVQILCVVAFCHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDV 300
           KTI+VQIL VVAF HLQG+VHRDLKPENFLF+KKDENTGLK+IDFGLSDFVKPDE LNDV
Sbjct: 241 KTILVQILNVVAFFHLQGVVHRDLKPENFLFVKKDENTGLKVIDFGLSDFVKPDEHLNDV 300

Query: 301 VGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDD 360
           VGSAYYVAPEVLYRSYS EAD+WSIGVIAYILLCG RPFWARTESGIFRSVLRADPNFDD
Sbjct: 301 VGSAYYVAPEVLYRSYSHEADVWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD 360

Query: 361 SPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRA 420
           SPWPTIS EAKDFVKRLLNKDHRKRMTA QALTHPWLRDE++AVPLD+FIYKSVKAYLR 
Sbjct: 361 SPWPTISAEAKDFVKRLLNKDHRKRMTAVQALTHPWLRDENVAVPLDSFIYKSVKAYLRV 420

Query: 421 TPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVTIDNY------------------ 480
           TPFKRAALKALAKALTEDELFYLR QFKLLEPQNGFVT+DN+                  
Sbjct: 421 TPFKRAALKALAKALTEDELFYLRAQFKLLEPQNGFVTLDNFKEALVRNATDAMRESNVL 480

Query: 481 ---KAMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELV 540
              K  E +A +RM FEEFCAAAISVYQLEAVAGWE IATRAFEYFEQ+GNRVISVHELV
Sbjct: 481 DILKMTESIAYERMDFEEFCAAAISVYQLEAVAGWEGIATRAFEYFEQDGNRVISVHELV 540

Query: 541 QEMNLGPIAHSFLQNWTRSSDGKLSFFGYTKFLHGVTIRYSNTRH 551
           QEMNL P AHSFLQNWTRSSDGKLSFFGYT+FLHGVT+R SNTRH
Sbjct: 541 QEMNLSPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTVRNSNTRH 585

BLAST of CmaCh15G010020 vs. NCBI nr
Match: XP_022974330.1 (CDPK-related kinase 6-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 550/571 (96.32%), Postives = 550/571 (96.32%), Query Frame = 0

Query: 1   MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60
           MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR
Sbjct: 1   MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60

Query: 61  TRRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAK 120
           TRRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAK
Sbjct: 61  TRRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAK 120

Query: 121 FELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKALS 180
           FELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKALS
Sbjct: 121 FELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKALS 180

Query: 181 GHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFC 240
           GHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFC
Sbjct: 181 GHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFC 240

Query: 241 HLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYR 300
           HLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYR
Sbjct: 241 HLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYR 300

Query: 301 SYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFV 360
           SYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFV
Sbjct: 301 SYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFV 360

Query: 361 KRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKA 420
           KRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKA
Sbjct: 361 KRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKA 420

Query: 421 LTEDELFYLRTQFKLLEPQNGFVTIDNYKA---------------------MEPLANKRM 480
           LTEDELFYLRTQFKLLEPQNGFVTIDNYKA                     MEPLANKRM
Sbjct: 421 LTEDELFYLRTQFKLLEPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRM 480

Query: 481 GFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQ 540
           GFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQ
Sbjct: 481 GFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQ 540

Query: 541 NWTRSSDGKLSFFGYTKFLHGVTIRYSNTRH 551
           NWTRSSDGKLSFFGYTKFLHGVTIRYSNTRH
Sbjct: 541 NWTRSSDGKLSFFGYTKFLHGVTIRYSNTRH 571

BLAST of CmaCh15G010020 vs. NCBI nr
Match: XP_023549645.1 (CDPK-related kinase 6-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1064.7 bits (2752), Expect = 2.7e-307
Identity = 538/572 (94.06%), Postives = 543/572 (94.93%), Query Frame = 0

Query: 1   MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60
           MGHHCSKSVSDVS+HA ISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPS IAPSPAR
Sbjct: 1   MGHHCSKSVSDVSNHAVISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSGIAPSPAR 60

Query: 61  T-RRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGA 120
           T RRKFRWPLPPPSPAKPIMAILR+KSRKASI EENQI EEGLGEVQLDKSFGYSTNFGA
Sbjct: 61  TPRRKFRWPLPPPSPAKPIMAILRRKSRKASIQEENQIREEGLGEVQLDKSFGYSTNFGA 120

Query: 121 KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKAL 180
           KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISK+KMTST+ IEDVRRE KILKAL
Sbjct: 121 KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTIAIEDVRREVKILKAL 180

Query: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240
           SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF
Sbjct: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240

Query: 241 CHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLY 300
           CHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLY
Sbjct: 241 CHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLY 300

Query: 301 RSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDF 360
           RSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDF
Sbjct: 301 RSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDF 360

Query: 361 VKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAK 420
           VKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAK
Sbjct: 361 VKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAK 420

Query: 421 ALTEDELFYLRTQFKLLEPQNGFVTIDNYKA---------------------MEPLANKR 480
           ALTEDELFYLRTQFKLLEPQNGFVTIDNYK                      MEPLANKR
Sbjct: 421 ALTEDELFYLRTQFKLLEPQNGFVTIDNYKTALVRNVTDAMRESNVADILKMMEPLANKR 480

Query: 481 MGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFL 540
           MGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFL
Sbjct: 481 MGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFL 540

Query: 541 QNWTRSSDGKLSFFGYTKFLHGVTIRYSNTRH 551
           QNWTRSSDGKLSFFGYT+FLHGVTIRYSNTRH
Sbjct: 541 QNWTRSSDGKLSFFGYTRFLHGVTIRYSNTRH 572

BLAST of CmaCh15G010020 vs. NCBI nr
Match: XP_022922250.1 (CDPK-related kinase 4 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1055.4 bits (2728), Expect = 1.6e-304
Identity = 534/572 (93.36%), Postives = 541/572 (94.58%), Query Frame = 0

Query: 1   MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60
           MGHHCSKSVSD+S+HA ISGGS P+     EDTRHSNSVSATASPFSSPLPS IAPSPAR
Sbjct: 1   MGHHCSKSVSDISNHAVISGGSHPT-----EDTRHSNSVSATASPFSSPLPSGIAPSPAR 60

Query: 61  -TRRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGA 120
            TRRKFRWPLPPPSPAKPIMAILR+KSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGA
Sbjct: 61  TTRRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGA 120

Query: 121 KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKAL 180
           KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISK+KMTSTVGIEDVRRE KILKAL
Sbjct: 121 KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKAL 180

Query: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240
           SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF
Sbjct: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240

Query: 241 CHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLY 300
           CHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLY
Sbjct: 241 CHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLY 300

Query: 301 RSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDF 360
           RSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDF
Sbjct: 301 RSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDF 360

Query: 361 VKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAK 420
           VKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAK
Sbjct: 361 VKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAK 420

Query: 421 ALTEDELFYLRTQFKLLEPQNGFVTIDNYKA---------------------MEPLANKR 480
           ALTEDELFYLRTQFKLL+PQNGFVTIDNYKA                     MEPLANKR
Sbjct: 421 ALTEDELFYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKR 480

Query: 481 MGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFL 540
           MGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFL
Sbjct: 481 MGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFL 540

Query: 541 QNWTRSSDGKLSFFGYTKFLHGVTIRYSNTRH 551
           QNWTRSSDGKLSFFGYT+FLHGVTIRYSNTRH
Sbjct: 541 QNWTRSSDGKLSFFGYTRFLHGVTIRYSNTRH 567

BLAST of CmaCh15G010020 vs. NCBI nr
Match: KAG7016799.1 (CDPK-related kinase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1018.8 bits (2633), Expect = 1.7e-293
Identity = 530/625 (84.80%), Postives = 539/625 (86.24%), Query Frame = 0

Query: 1   MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60
           MGHHCSKSVSD+S+HA ISGGS P+     EDTRHSNSVSATASPFSSPLPS IAPSPAR
Sbjct: 1   MGHHCSKSVSDISNHAVISGGSHPT-----EDTRHSNSVSATASPFSSPLPSGIAPSPAR 60

Query: 61  T-RRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGA 120
           T RRKFRWPLPPPSPAKPIMAILR+KSRKASIPEENQIGEEGLGEVQLDKSFGYS NFGA
Sbjct: 61  TPRRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGA 120

Query: 121 KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKAL 180
           KFELG+EVGRGHFGHTFWAKGKKGDLKGKPVAVKIISK+KMTSTVGIEDVRRE KILKAL
Sbjct: 121 KFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKAL 180

Query: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240
           SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF
Sbjct: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240

Query: 241 CHLQGIVHRDLKPE--------NFLFMKKDENTGLKLIDFGLSDFVKP------------ 300
           CHLQGIVHRDLKPE        NFLFMKKDENTGLKLIDFGLSDFVKP            
Sbjct: 241 CHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWA 300

Query: 301 ---------------------------------DERLNDVVGSAYYVAPEVLYRSYSFEA 360
                                            DERLNDVVGSAYYVAPEVLYRSYSFEA
Sbjct: 301 VLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEA 360

Query: 361 DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK 420
           DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK
Sbjct: 361 DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK 420

Query: 421 DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDEL 480
           DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL ATPFKRAALKALAKALTEDEL
Sbjct: 421 DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDEL 480

Query: 481 FYLRTQFKLLEPQNGFVTIDNYKA---------------------MEPLANKRMGFEEFC 540
           FYLRTQFKLL+PQNGFVTIDNYKA                     MEPLANKRMGFEEFC
Sbjct: 481 FYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC 540

Query: 541 AAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQNWTRSS 551
           AAAISVYQLEAVAGWESIATRAFEYFEQ+GNRVISVHELVQEMNLGPIAHSFLQNWTRSS
Sbjct: 541 AAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSS 600

BLAST of CmaCh15G010020 vs. NCBI nr
Match: XP_022974329.1 (CDPK-related kinase 6-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1001.5 bits (2588), Expect = 2.8e-288
Identity = 507/530 (95.66%), Postives = 507/530 (95.66%), Query Frame = 0

Query: 1   MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60
           MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR
Sbjct: 1   MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60

Query: 61  TRRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAK 120
           TRRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAK
Sbjct: 61  TRRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAK 120

Query: 121 FELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKALS 180
           FELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKALS
Sbjct: 121 FELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKALS 180

Query: 181 GHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFC 240
           GHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFC
Sbjct: 181 GHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFC 240

Query: 241 HLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYR 300
           HLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYR
Sbjct: 241 HLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYR 300

Query: 301 SYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFV 360
           SYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFV
Sbjct: 301 SYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFV 360

Query: 361 KRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKA 420
           KRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKA
Sbjct: 361 KRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKA 420

Query: 421 LTEDELFYLRTQFKLLEPQNGFVTIDNYKA---------------------MEPLANKRM 480
           LTEDELFYLRTQFKLLEPQNGFVTIDNYKA                     MEPLANKRM
Sbjct: 421 LTEDELFYLRTQFKLLEPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRM 480

Query: 481 GFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMN 510
           GFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQ  N
Sbjct: 481 GFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQVRN 530

BLAST of CmaCh15G010020 vs. TAIR 10
Match: AT3G49370.1 (Calcium-dependent protein kinase (CDPK) family protein )

HSP 1 Score: 693.3 bits (1788), Expect = 1.5e-199
Identity = 359/592 (60.64%), Postives = 445/592 (75.17%), Query Frame = 0

Query: 1   MGHHCSKSVSDVS-------------HHAAISGGSRPSSSPIPEDTRHSNSVSATASPFS 60
           MGH  S+++S V              H +  +     SSS IP+    S       SPF 
Sbjct: 1   MGHCYSRNISTVDDDDEIPSATAQLPHRSHQNHHQTSSSSSIPQSPATSEVNPYNISPFQ 60

Query: 61  SPLPSRIAPSPART-RRKFRWPLPPPSPAKPIMAILRQK------SRKASIPEENQIGEE 120
           SPLP+ +APSPART  RKF+WP PPPSPAKPIMA LR++       R   IPE+++ G  
Sbjct: 61  SPLPAGVAPSPARTPGRKFKWPFPPPSPAKPIMAALRRRRGTAPHPRDGPIPEDSEAGGS 120

Query: 121 --GLGEVQLDKSFGYSTNFGAKFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKT 180
             G+GE +LDK+FG++ NF  K+ELGREVGRGHFGHT WAK KKG +KG+ VAVKIISK+
Sbjct: 121 GGGIGE-RLDKNFGFAKNFEGKYELGREVGRGHFGHTCWAKAKKGKIKGQTVAVKIISKS 180

Query: 181 KMTSTVGIEDVRREEKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRG 240
           KMTS + IEDVRRE K+LKALSGH ++V F+  FED++N+++VMELCEGGELL+ I++RG
Sbjct: 181 KMTSALSIEDVRREVKLLKALSGHSHMVKFYDVFEDSDNVFVVMELCEGGELLDSILARG 240

Query: 241 GRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVK 300
           GRY E E+K I+VQIL   AF HLQG+VHRDLKPENFLF  K+E+  LK+IDFGLSD+ +
Sbjct: 241 GRYPEAEAKRILVQILSATAFFHLQGVVHRDLKPENFLFTSKNEDAVLKVIDFGLSDYAR 300

Query: 301 PDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVL 360
            D+RLNDVVGSAYYVAPEVL+RSYS EAD+WSIGVI+YILLCGSRPF+ RTES IFR VL
Sbjct: 301 FDQRLNDVVGSAYYVAPEVLHRSYSTEADIWSIGVISYILLCGSRPFYGRTESAIFRCVL 360

Query: 361 RADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYK 420
           RA+PNFDD PWP+ISP AKDFVKRLLNKDHRKRMTAAQAL HPWLRDE+  + LD  IYK
Sbjct: 361 RANPNFDDLPWPSISPIAKDFVKRLLNKDHRKRMTAAQALAHPWLRDENPGLLLDFSIYK 420

Query: 421 SVKAYLRATPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVTIDNY---------- 480
            VK+Y+RA+PF+RAALK+L+KA+ E+EL +L+ QF LLEP++G + + N+          
Sbjct: 421 LVKSYIRASPFRRAALKSLSKAIPEEELVFLKAQFMLLEPEDGGLHLHNFTTALTRYATD 480

Query: 481 -----------KAMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNR 540
                        M+PLA+K++ FEEFCAA++SVYQLEA+  WE IAT AFE+FE EG+R
Sbjct: 481 AMIESRLPDILNMMQPLAHKKLDFEEFCAASVSVYQLEALEEWEQIATVAFEHFESEGSR 540

Query: 541 VISVHELVQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTKFLHGVTIRYSNTR 550
            ISV EL +EM+LGP A+  L++W RS DGKL+F GY KFLHGVT+R S++R
Sbjct: 541 AISVQELAEEMSLGPNAYPLLKDWIRSLDGKLNFLGYAKFLHGVTVRSSSSR 591

BLAST of CmaCh15G010020 vs. TAIR 10
Match: AT5G24430.1 (Calcium-dependent protein kinase (CDPK) family protein )

HSP 1 Score: 692.6 bits (1786), Expect = 2.6e-199
Identity = 358/593 (60.37%), Postives = 447/593 (75.38%), Query Frame = 0

Query: 1   MGHHCSKSVSDVSHHAAISGGSRPSSSP--------IPEDTRHSNSV---SATASPFSSP 60
           MGH  S+++S V      +G    S+ P        IP+    S +    S   SPF SP
Sbjct: 1   MGHCYSRNISAVEDDEIPTGNGEVSNQPSQNHRHASIPQSPVASGTPEVNSYNISPFQSP 60

Query: 61  LPSRIAPSPART-RRKFRWPLPPPSPAKPIMAILRQK------SRKASIPEENQI----- 120
           LP+ +APSPART  RKF+WP PPPSPAKPIMA LR++       R   IPE+++      
Sbjct: 61  LPAGVAPSPARTPGRKFKWPFPPPSPAKPIMAALRRRRGAPPQPRDEPIPEDSEDVVDHG 120

Query: 121 GEEGLGEVQLDKSFGYSTNFGAKFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISK 180
           G+ G GE +LDK+FG+  NF  K+ELG+EVGRGHFGHT WAK KKG +K + VAVKIISK
Sbjct: 121 GDSGGGE-RLDKNFGFGKNFEGKYELGKEVGRGHFGHTCWAKAKKGKMKNQTVAVKIISK 180

Query: 181 TKMTSTVGIEDVRREEKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSR 240
            KMTST+ IEDVRRE K+LKALSGH+++V F+  +EDA+N+++VMELCEGGELL+RI++R
Sbjct: 181 AKMTSTLSIEDVRREVKLLKALSGHRHMVKFYDVYEDADNVFVVMELCEGGELLDRILAR 240

Query: 241 GGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFV 300
           GGRY E ++K I+VQIL   AF HLQG+VHRDLKPENFLF  ++E+  LK+IDFGLSDF+
Sbjct: 241 GGRYPEVDAKRILVQILSATAFFHLQGVVHRDLKPENFLFTSRNEDAILKVIDFGLSDFI 300

Query: 301 KPDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSV 360
           + D+RLNDVVGSAYYVAPEVL+RSYS EADMWSIGVI+YILLCGSRPF+ RTES IFR V
Sbjct: 301 RYDQRLNDVVGSAYYVAPEVLHRSYSTEADMWSIGVISYILLCGSRPFYGRTESAIFRCV 360

Query: 361 LRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIY 420
           LRA+PNF+D PWP+ISP AKDFVKRLLNKDHRKRMTAAQAL HPWLRDE+  + LD  +Y
Sbjct: 361 LRANPNFEDMPWPSISPTAKDFVKRLLNKDHRKRMTAAQALAHPWLRDENPGLLLDFSVY 420

Query: 421 KSVKAYLRATPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVTI------------ 480
           K VK+Y+RA+PF+R+ALKAL+KA+ ++EL +L+ QF LL+P++G +++            
Sbjct: 421 KLVKSYIRASPFRRSALKALSKAIPDEELVFLKAQFMLLDPKDGGLSLNCFTMALTRYAT 480

Query: 481 ---------DNYKAMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGN 540
                    D    M+PLA K++ FEEFCAAA+SVYQLEA+  WE IAT AFE+FE EGN
Sbjct: 481 DAMMESRLPDILNTMQPLAQKKLDFEEFCAAAVSVYQLEALEEWEQIATSAFEHFEHEGN 540

Query: 541 RVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTKFLHGVTIRYSNTR 550
           R+ISV EL  EM++GP A+  L++W RSSDGKLSF GY KFLHGVT+R S++R
Sbjct: 541 RIISVQELAGEMSVGPSAYPLLKDWIRSSDGKLSFLGYAKFLHGVTVRSSSSR 592

BLAST of CmaCh15G010020 vs. TAIR 10
Match: AT2G46700.1 (CDPK-related kinase 3 )

HSP 1 Score: 687.2 bits (1772), Expect = 1.1e-197
Identity = 352/547 (64.35%), Postives = 425/547 (77.70%), Query Frame = 0

Query: 36  SNSVSATASPF----SSPLPSRIAPSPART---RRKFRWPLPPPSPAKPIMAILRQ---- 95
           S++ S   SPF    +SPLPS ++PSPART   RR FR P PPPSPAK I A L +    
Sbjct: 51  SSNPSPWPSPFPHGSASPLPSGVSPSPARTSTPRRFFRRPFPPPSPAKHIKASLIKRLGV 110

Query: 96  KSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAKFELGREVGRGHFGHTFWAKGKKGD 155
           K ++  IPEE   G E   E  LDKSFGY  NFGAK+ELG+EVGRGHFGHT   +GKKGD
Sbjct: 111 KPKEGPIPEER--GTE--PEQSLDKSFGYGKNFGAKYELGKEVGRGHFGHTCSGRGKKGD 170

Query: 156 LKGKPVAVKIISKTKMTSTVGIEDVRREEKILKALSGHKNLVHFHGAFEDANNIYIVMEL 215
           +K  P+AVKIISK KMT+ + IEDVRRE K+LK+LSGHK L+ ++ A EDANN+YIVMEL
Sbjct: 171 IKDHPIAVKIISKAKMTTAIAIEDVRREVKLLKSLSGHKYLIKYYDACEDANNVYIVMEL 230

Query: 216 CEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPENFLFMKKDENT 275
           C+GGELL+RI++RGG+Y E ++K I+VQIL VV+FCHLQG+VHRDLKPENFLF    E++
Sbjct: 231 CDGGELLDRILARGGKYPEDDAKAIVVQILTVVSFCHLQGVVHRDLKPENFLFTSSREDS 290

Query: 276 GLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRP 335
            LKLIDFGLSDF++PDERLND+VGSAYYVAPEVL+RSYS EAD+WSIGVI YILLCGSRP
Sbjct: 291 DLKLIDFGLSDFIRPDERLNDIVGSAYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRP 350

Query: 336 FWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLR 395
           FWARTESGIFR+VLR +PN+DD PWP+ S E KDFVKRLLNKD+RKRM+A QALTHPWLR
Sbjct: 351 FWARTESGIFRTVLRTEPNYDDVPWPSCSSEGKDFVKRLLNKDYRKRMSAVQALTHPWLR 410

Query: 396 DEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDELFYLRTQFKLLEP-QNGFV 455
           D+   +PLD  IYK VKAYL ATP +RAALKALAKALTE+EL YLR QF LL P ++G V
Sbjct: 411 DDSRVIPLDILIYKLVKAYLHATPLRRAALKALAKALTENELVYLRAQFMLLGPNKDGSV 470

Query: 456 TIDNYK---------------------AMEPLANKRMGFEEFCAAAISVYQLEAVAGWES 515
           +++N+K                      ME LA ++M FEEFCAAAIS++QLEAV  WE 
Sbjct: 471 SLENFKTALMQNATDAMRESRVPEILHTMESLAYRKMYFEEFCAAAISIHQLEAVDAWEE 530

Query: 516 IATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTKFLHGVT 550
           IAT  F++FE EGNRVI++ EL +E+N+G  A+  L++W RSSDGKLS+ G+TKFLHGVT
Sbjct: 531 IATAGFQHFETEGNRVITIEELARELNVGASAYGHLRDWVRSSDGKLSYLGFTKFLHGVT 590

BLAST of CmaCh15G010020 vs. TAIR 10
Match: AT3G50530.1 (CDPK-related kinase )

HSP 1 Score: 622.9 bits (1605), Expect = 2.5e-178
Identity = 321/543 (59.12%), Postives = 404/543 (74.40%), Query Frame = 0

Query: 45  PFSSPLPSRI-------APSPART------RRKFRWPLPPPSPAKPIMAILRQ-----KS 104
           PF SP P+         A SPA        +R F+ P PPPSPAK I A+L +     K 
Sbjct: 57  PFYSPSPAHYFFSKKTPARSPATNSTNSTPKRFFKRPFPPPSPAKHIRAVLARRHGSVKP 116

Query: 105 RKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAKFELGREVGRGHFGHTFWAKGKKGDLK 164
             ++IPE ++    G+G   LDKSFG+S +F +K+ELG EVGRGHFG+T  AK KKGD K
Sbjct: 117 NSSAIPEGSEAEGGGVG---LDKSFGFSKSFASKYELGDEVGRGHFGYTCAAKFKKGDNK 176

Query: 165 GKPVAVKIISKTKMTSTVGIEDVRREEKILKALSGHKNLVHFHGAFEDANNIYIVMELCE 224
           G+ VAVK+I K KMT+ + IEDVRRE KIL+ALSGH NL HF+ A+ED +N+YIVMELCE
Sbjct: 177 GQQVAVKVIPKAKMTTAIAIEDVRREVKILRALSGHNNLPHFYDAYEDHDNVYIVMELCE 236

Query: 225 GGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPENFLFMKKDENTGL 284
           GGELL+RI+SRGG+Y E+++KT+++QIL VVAFCHLQG+VHRDLKPENFLF  K++ + L
Sbjct: 237 GGELLDRILSRGGKYTEEDAKTVMIQILNVVAFCHLQGVVHRDLKPENFLFTSKEDTSQL 296

Query: 285 KLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFW 344
           K IDFGLSD+V+PDERLND+VGSAYYVAPEVL+RSYS EAD+WS+GVI YILLCGSRPFW
Sbjct: 297 KAIDFGLSDYVRPDERLNDIVGSAYYVAPEVLHRSYSTEADIWSVGVIVYILLCGSRPFW 356

Query: 345 ARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRD- 404
           ARTESGIFR+VL+ADP+FDD PWP +S EA+DFVKRLLNKD RKR+TAAQAL+HPW++D 
Sbjct: 357 ARTESGIFRAVLKADPSFDDPPWPLLSSEARDFVKRLLNKDPRKRLTAAQALSHPWIKDS 416

Query: 405 EDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDELFYLRTQFKLLEP-QNGFVT 464
            D  VP+D  ++K ++AYLR++  ++AAL+AL+K LT DELFYLR QF LLEP +NG ++
Sbjct: 417 NDAKVPMDILVFKLMRAYLRSSSLRKAALRALSKTLTVDELFYLREQFALLEPSKNGTIS 476

Query: 465 IDNYKA---------------------MEPLANKRMGFEEFCAAAISVYQLEAVAGWESI 524
           ++N K+                     +  L  +RM FEEFCAAA+SV+QLEA+  WE  
Sbjct: 477 LENIKSALMKMATDAMKDSRIPEFLGQLSALQYRRMDFEEFCAAALSVHQLEALDRWEQH 536

Query: 525 ATRAFEYFEQEGNRVISVHELVQEMNLGPI--AHSFLQNWTRSSDGKLSFFGYTKFLHGV 545
           A  A+E FE+EGNR I + EL  E+ LGP    H+ L +W R +DGKLSF G+ K LHGV
Sbjct: 537 ARCAYELFEKEGNRPIMIDELASELGLGPSVPVHAVLHDWLRHTDGKLSFLGFVKLLHGV 596

BLAST of CmaCh15G010020 vs. TAIR 10
Match: AT2G41140.1 (CDPK-related kinase 1 )

HSP 1 Score: 608.6 bits (1568), Expect = 4.9e-174
Identity = 320/554 (57.76%), Postives = 398/554 (71.84%), Query Frame = 0

Query: 16  AAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPARTRRKFRWPLPPPSPA 75
           A  SG    S SP+P   + S SVS++ S  S+PL            R F+ P PPPSPA
Sbjct: 33  AKSSGFPFYSPSPVPSLFKSSPSVSSSVS--STPL------------RIFKRPFPPPSPA 92

Query: 76  KPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAKFELGREVGRGHFGHT 135
           K I A L ++      P E  I E    E+ LDKSFG+S  F + +E+  EVGRGHFG+T
Sbjct: 93  KHIRAFLARRYGSVK-PNEVSIPEGKECEIGLDKSFGFSKQFASHYEIDGEVGRGHFGYT 152

Query: 136 FWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKALSGHKNLVHFHGAFEDA 195
             AKGKKG LKG+ VAVK+I K+KMT+ + IEDV RE K+L+AL+GHKNLV F+ AFED 
Sbjct: 153 CSAKGKKGSLKGQEVAVKVIPKSKMTTAIAIEDVSREVKMLRALTGHKNLVQFYDAFEDD 212

Query: 196 NNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPENF 255
            N+YIVMELC+GGELL++I+ RGG+Y E ++K ++VQIL VVA+CHLQG+VHRDLKPENF
Sbjct: 213 ENVYIVMELCKGGELLDKILQRGGKYSEDDAKKVMVQILSVVAYCHLQGVVHRDLKPENF 272

Query: 256 LFMKKDENTGLKLIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIA 315
           LF  KDE + LK IDFGLSD+VKPDERLND+VGSAYYVAPEVL+R+Y  EADMWSIGVIA
Sbjct: 273 LFSTKDETSPLKAIDFGLSDYVKPDERLNDIVGSAYYVAPEVLHRTYGTEADMWSIGVIA 332

Query: 316 YILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAA 375
           YILLCGSRPFWARTESGIFR+VL+A+PNF+++PWP++SPEA DFVKRLLNKD+RKR+TAA
Sbjct: 333 YILLCGSRPFWARTESGIFRAVLKAEPNFEEAPWPSLSPEAVDFVKRLLNKDYRKRLTAA 392

Query: 376 QALTHPWL-RDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDELFYLRTQFK 435
           QAL HPWL    +L +P D  IYK VK Y+ +T  +++AL ALAK LT  +L YLR QF 
Sbjct: 393 QALCHPWLVGSHELKIPSDMIIYKLVKVYIMSTSLRKSALAALAKTLTVPQLAYLREQFT 452

Query: 436 LLEP-QNGFVTIDNYKA---------------------MEPLANKRMGFEEFCAAAISVY 495
           LL P +NG++++ NYK                      +  L  K++ FEEFCA+A+SVY
Sbjct: 453 LLGPSKNGYISMQNYKTAILKSSTDAMKDSRVFDFVHMISCLQYKKLDFEEFCASALSVY 512

Query: 496 QLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPI--AHSFLQNWTRSSDGKLS 545
           QLEA+  WE  A RA+E FE++GNR I + EL  E+ LGP    H  LQ+W R SDGKLS
Sbjct: 513 QLEAMETWEQHARRAYELFEKDGNRPIMIEELASELGLGPSVPVHVVLQDWIRHSDGKLS 571

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SG122.1e-19860.64CDPK-related kinase 6 OS=Arabidopsis thaliana OX=3702 GN=CRK6 PE=1 SV=1[more]
Q9FIM93.7e-19860.37CDPK-related kinase 4 OS=Arabidopsis thaliana OX=3702 GN=CRK4 PE=1 SV=1[more]
Q9ZUZ21.5e-19664.35CDPK-related kinase 3 OS=Arabidopsis thaliana OX=3702 GN=CRK3 PE=1 SV=1[more]
Q9SCS23.6e-17759.12CDPK-related kinase 5 OS=Arabidopsis thaliana OX=3702 GN=CRK5 PE=2 SV=1[more]
Q10KY33.1e-17358.62Calcium/calmodulin-dependent serine/threonine-protein kinase 1 OS=Oryza sativa s... [more]
Match NameE-valueIdentityDescription
A0A6J1IDP00.0e+0096.32CDPK-related kinase 6-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11147296... [more]
A0A6J1E8747.9e-30593.36CDPK-related kinase 4 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430287 P... [more]
A0A6J1IHA71.4e-28895.66CDPK-related kinase 6-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11147296... [more]
A0A6J1E2V01.8e-27792.66CDPK-related kinase 4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430287 P... [more]
A0A6J1K6B24.7e-27383.08CDPK-related kinase 6-like OS=Cucurbita maxima OX=3661 GN=LOC111491067 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022974330.10.0e+0096.32CDPK-related kinase 6-like isoform X2 [Cucurbita maxima][more]
XP_023549645.12.7e-30794.06CDPK-related kinase 6-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022922250.11.6e-30493.36CDPK-related kinase 4 isoform X2 [Cucurbita moschata][more]
KAG7016799.11.7e-29384.80CDPK-related kinase 4, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022974329.12.8e-28895.66CDPK-related kinase 6-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G49370.11.5e-19960.64Calcium-dependent protein kinase (CDPK) family protein [more]
AT5G24430.12.6e-19960.37Calcium-dependent protein kinase (CDPK) family protein [more]
AT2G46700.11.1e-19764.35CDPK-related kinase 3 [more]
AT3G50530.12.5e-17859.12CDPK-related kinase [more]
AT2G41140.14.9e-17457.76CDPK-related kinase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 121..383
e-value: 1.6E-96
score: 336.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 121..383
e-value: 1.5E-70
score: 237.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 121..383
score: 50.548145
NoneNo IPR availableGENE3D1.10.238.10coord: 397..534
e-value: 3.8E-7
score: 32.2
NoneNo IPR availableGENE3D1.10.238.10coord: 422..469
e-value: 3.8E-7
score: 32.2
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 117..206
e-value: 9.2E-21
score: 75.8
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 207..395
e-value: 6.2E-59
score: 201.0
NoneNo IPR availablePIRSRPIRSR000556-2PIRSR000556-2coord: 121..325
e-value: 6.4E-19
score: 65.7
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 120..338
e-value: 1.5E-8
score: 31.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..76
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 12..50
NoneNo IPR availablePANTHERPTHR24349:SF361CDPK-RELATED KINASE 3coord: 47..544
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 47..544
NoneNo IPR availableCDDcd05117STKc_CAMKcoord: 120..382
e-value: 1.8846E-121
score: 356.016
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 245..257
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 127..153
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 415..540
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 120..400

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh15G010020.1CmaCh15G010020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0009931 calcium-dependent protein serine/threonine kinase activity
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity
molecular_function GO:0004672 protein kinase activity