CmaCh15G006840 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh15G006840
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	RNA polymerase II transcription factor B subunit 4
Location	Cma_Chr15: 3274253 .. 3275818 (+)
RNA-Seq Expression	CmaCh15G006840
Synteny	CmaCh15G006840

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATAACCAACATAGGTGTTGCATGCTGCCGAAAATTGACAAACCTCATGCAGCTCCGATTGCTAAGCACGCCGACCCTTGCTCCTCATCGGTCGAAGGGAAGCTTCAAGTCGAGACCCTCGAAATGAATATCAAATCAGGTATGTGCAGTTTACTGTTGTGTGGGAAGGCAGCTCAAAGCAGAGGTCAAGACCAACACCTGCTGATTGTTTCACTGGGAGGTTTGTCTGTGAAGTAAAAACTGGCTTCAGCACATCAAAAGCATCCTCTATTAATCATATATTAGACATTTATAATTTTTTTAGTTTCATATAATTATATCATATATTATACATGAAAAAAAAATATCGGAAATTAAAAAAAAAAAAAAAAAAAAAAACTTTATTTTCTTTAATTGCTCTTTAAGATGAACTTCATATAAAGGCCTATGGGGCCGCCAAACAAAAAATAATTGAGCCGACAGTTCGGCCCAATTTGTAAACATTGGGCCCATATTCTATAATGGGCCGTTTTCTCAGTGGGCTGTTTGTACAGTTTATGGCCCAATTCCCATTTACAGTCTGGGAAATCGCACGGCTGAAACATACATCGTCGACTACTCCACACCTGAAAATCCGAAGGTTTGTTTGTTGCAGAAACACACCACGCCGTCGACTGCTTCAACCCAGCCGTTGCCGTTACCGTTGCCGGTGCCGTTTTCATCGTCGATTGCTGCGACCCTTGCCGTTGAGGAAGACCAATCAGATTGCAAGCTTGGCGTCTCCTTCTGTCCACGGCGCCGAAAGGTAAGTTTCTTCCCTTTTTATTTGTTTATTTATGATGGTGGGTAGAACGGGGCGTCTGTGGGTCAATCTGTAACATTTTTGGCCCATTTTCCGTCGAAGTCTATTAGAATTAGAACTGGGCGTCTTCTCTCCGTTGCCAATTCAGAAACAGCCATGGCCTCTGCTCCTTCCAAGCTCTATGCAGGTTCTTCTTCAATTCCTCTCTCCTTTCATATGGATTCTCAGGTACGAGTATCAGAGTTTCAGGGGCTTATTCTTTTTTGTGATCTTTTGATTGTGTTGCTGCAGATGATGTTAGCCTGTTAGTGGTTTTACTGGATACAAATCCATTTTTCTGGAGCACATATTCTCTCCCATTCTCCAAGTTCCTCTCTCATGTACTTGATTATTTCTCTCTCTACTTATGGATCATTCTATCAAATTGGTATTTCAGGTAGAAATGTAATGAACACTGGTAATTTTCTGTTTTGGGTATCTAGGTACTTGCTTTTCTGAACTCCATTTTAGTTCTGAATCAACTTAATGAGGTTGTGGTCATTGGTACCGGATATGCTTCATGCAAGTATCTATACAACTCGTCTTCTTACTCAAATCGTAGCCTTGAAGATGGTAGAATGCCTGCACTTTGCACTCGTTTATTGAATAATTTGGAGGAATTCATGATTGGGGATGAGCAGTCAATCAAGGAAGACCCCAGAGAAGGGACCATGTCTTCACTTCTTTCTGGATCGCTATCCATGGCTTTGTGTTGTATCCTTGTTGATGATTTTTGGTGCTGA

mRNA sequence

ATGAATAACCAACATAGGTGTTGCATGCTGCCGAAAATTGACAAACCTCATGCAGCTCCGATTGCTAAGCACGCCGACCCTTGCTCCTCATCGGTCGAAGGGAAGCTTCAAGTCGAGACCCTCGAAATGAATATCAAATCAGGTATGTGCAGTTTACTGTTGTGTGGGAAGGCAGCTCAAAGCAGAGGTCAAGACCAACACCTGCTGATTGTTTCACTGGGAGTTTATGGCCCAATTCCCATTTACAGTCTGGGAAATCGCACGGCTGAAACATACATCGTCGACTACTCCACACCTGAAAATCCGAAGGTTTGTTTGTTGCAGAAACACACCACGCCGTCGACTGCTTCAACCCAGCCGTTGCCGTTACCGTTGCCGGTGCCGTTTTCATCGTCGATTGCTGCGACCCTTGCCGTTGAGGAAGACCAATCAGATTGCAAGCTTGGCGTCTCCTTCTGTCCACGGCGCCGAAAGTCTATTAGAATTAGAACTGGGCGTCTTCTCTCCGTTGCCAATTCAGAAACAGCCATGGCCTCTGCTCCTTCCAAGCTCTATGCAGATGATGTTAGCCTGTTAGTGGTTTTACTGGATACAAATCCATTTTTCTGGAGCACATATTCTCTCCCATTCTCCAAGTTCCTCTCTCATGTACTTGCTTTTCTGAACTCCATTTTAGTTCTGAATCAACTTAATGAGGTTGTGGTCATTGGTACCGGATATGCTTCATGCAAGTATCTATACAACTCGTCTTCTTACTCAAATCGTAGCCTTGAAGATGGTAGAATGCCTGCACTTTGCACTCGTTTATTGAATAATTTGGAGGAATTCATGATTGGGGATGAGCAGTCAATCAAGGAAGACCCCAGAGAAGGGACCATGTCTTCACTTCTTTCTGGATCGCTATCCATGGCTTTGTGTTGTATCCTTGTTGATGATTTTTGGTGCTGA

Coding sequence (CDS)

ATGAATAACCAACATAGGTGTTGCATGCTGCCGAAAATTGACAAACCTCATGCAGCTCCGATTGCTAAGCACGCCGACCCTTGCTCCTCATCGGTCGAAGGGAAGCTTCAAGTCGAGACCCTCGAAATGAATATCAAATCAGGTATGTGCAGTTTACTGTTGTGTGGGAAGGCAGCTCAAAGCAGAGGTCAAGACCAACACCTGCTGATTGTTTCACTGGGAGTTTATGGCCCAATTCCCATTTACAGTCTGGGAAATCGCACGGCTGAAACATACATCGTCGACTACTCCACACCTGAAAATCCGAAGGTTTGTTTGTTGCAGAAACACACCACGCCGTCGACTGCTTCAACCCAGCCGTTGCCGTTACCGTTGCCGGTGCCGTTTTCATCGTCGATTGCTGCGACCCTTGCCGTTGAGGAAGACCAATCAGATTGCAAGCTTGGCGTCTCCTTCTGTCCACGGCGCCGAAAGTCTATTAGAATTAGAACTGGGCGTCTTCTCTCCGTTGCCAATTCAGAAACAGCCATGGCCTCTGCTCCTTCCAAGCTCTATGCAGATGATGTTAGCCTGTTAGTGGTTTTACTGGATACAAATCCATTTTTCTGGAGCACATATTCTCTCCCATTCTCCAAGTTCCTCTCTCATGTACTTGCTTTTCTGAACTCCATTTTAGTTCTGAATCAACTTAATGAGGTTGTGGTCATTGGTACCGGATATGCTTCATGCAAGTATCTATACAACTCGTCTTCTTACTCAAATCGTAGCCTTGAAGATGGTAGAATGCCTGCACTTTGCACTCGTTTATTGAATAATTTGGAGGAATTCATGATTGGGGATGAGCAGTCAATCAAGGAAGACCCCAGAGAAGGGACCATGTCTTCACTTCTTTCTGGATCGCTATCCATGGCTTTGTGTTGTATCCTTGTTGATGATTTTTGGTGCTGA

Protein sequence

MNNQHRCCMLPKIDKPHAAPIAKHADPCSSSVEGKLQVETLEMNIKSGMCSLLLCGKAAQSRGQDQHLLIVSLGVYGPIPIYSLGNRTAETYIVDYSTPENPKVCLLQKHTTPSTASTQPLPLPLPVPFSSSIAATLAVEEDQSDCKLGVSFCPRRRKSIRIRTGRLLSVANSETAMASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVIGTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIKEDPREGTMSSLLSGSLSMALCCILVDDFWC

Homology

[Close]

BLAST of CmaCh15G006840 vs. ExPASy Swiss-Prot
Match: Q8LF41 (General transcription and DNA repair factor IIH subunit TFB4 OS=Arabidopsis thaliana OX=3702 GN=TFB4 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.1e-33
Identity = 82/136 (60.29%), Postives = 99/136 (72.79%), Query Frame = 0


Query: 177 MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 236
           M +  SK Y+DDVSLLV+LLDTNP FWST S+ FS+FLSHVLAFLN++L LNQLN+VVVI
Sbjct: 1   MPAIASKQYSDDVSLLVLLLDTNPLFWSTTSITFSQFLSHVLAFLNAVLGLNQLNQVVVI 60

Query: 237 GTGYASCKYLYNSSSYSNRSLEDGR---MPALCTRLLNNLEEFMIGDEQSIKEDPREGTM 296
            TGY+SC Y+Y+SS  SN    +     MPA+   LL  LEEF+  DE+  KE+  E  +
Sbjct: 61  ATGYSSCDYIYDSSLTSNHGNFESNGTGMPAIFGSLLKKLEEFVTKDEELSKEEVSEDRI 120

Query: 297 -SSLLSGSLSMALCCI 309
            S LLSGSLSMALC I
Sbjct: 121 PSCLLSGSLSMALCYI 136

[Close]

BLAST of CmaCh15G006840 vs. ExPASy Swiss-Prot
Match: O74366 (General transcription and DNA repair factor IIH subunit tfb4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tfb4 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 3.8e-06
Identity = 38/125 (30.40%), Postives = 63/125 (50.40%), Query Frame = 0


Query: 187 DDVSLLVVLLDTNPFFWSTYS--LPFSKFLSHVLAFLNSILVLNQLNEVVVIGTGYASCK 246
           D  SLLVV+LD NP  W + S  +P SK L+ +  FLN+ L  +  N V V+ +     +
Sbjct: 21  DTPSLLVVILDANPASWYSLSKKVPVSKVLADITVFLNAHLAFHHDNRVAVLASHSDKVE 80

Query: 247 YLYNSSSYSNRSLE-DGRMPALCTRLLNNLEEFMIGDEQSIKEDPREGTMSSLLSGSLSM 306
           YLY S +   +  E D    A   R    +++ ++   + +     + +  +++SG+LS 
Sbjct: 81  YLYPSIAPEQKVAEVDPTKEANTYRKFREVDDLVLSGMKRLMSSTDKVSRKTMISGALSR 140

Query: 307 ALCCI 309
           AL  I
Sbjct: 141 ALAYI 145

[Close]

BLAST of CmaCh15G006840 vs. ExPASy Swiss-Prot
Match: Q13889 (General transcription factor IIH subunit 3 OS=Homo sapiens OX=9606 GN=GTF2H3 PE=1 SV=2)

HSP 1 Score: 48.9 bits (115), Expect = 1.2e-04
Identity = 38/144 (26.39%), Postives = 70/144 (48.61%), Query Frame = 0


Query: 187 DDVSLLVVLLDTNPFFWSTYSLPFSKF-----LSHVLAFLNSILVLNQLNEVVVIGTGYA 246
           D+++LLV+++D NP +W   +L  S+F     +  V+   NS L +N+ N++ VI +   
Sbjct: 6   DELNLLVIVVDANPIWWGKQALKESQFTLSKCIDAVMVLGNSHLFMNRSNKLAVIASHIQ 65

Query: 247 SCKYLY---------------NSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSI-- 306
             ++LY               N   ++    +DG+       LL +  E ++ + + +  
Sbjct: 66  ESRFLYPGKNGRLGDFFGDPGNPPEFNPSGSKDGKY-----ELLTSANEVIVEEIKDLMT 125

Query: 307 KEDPREGTMSSLLSGSLSMALCCI 309
           K D +     +LL+GSL+ ALC I
Sbjct: 126 KSDIKGQHTETLLAGSLAKALCYI 144

[Close]

BLAST of CmaCh15G006840 vs. ExPASy Swiss-Prot
Match: Q05B56 (General transcription factor IIH subunit 3 OS=Bos taurus OX=9913 GN=GTF2H3 PE=2 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 1.6e-04
Identity = 40/143 (27.97%), Postives = 66/143 (46.15%), Query Frame = 0


Query: 187 DDVSLLVVLLDTNPFFWSTYSLPFSKF-----LSHVLAFLNSILVLNQLNEVVVIGTGYA 246
           D+++LLV+++DTNP +W   +L  S+F     +  V+   NS L +N+ N++ VI +   
Sbjct: 6   DELNLLVIIVDTNPIWWGKQALKESQFTLSKCIDAVMVLGNSHLFMNRSNKLAVIASHIQ 65

Query: 247 SCKYLY----------------NSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIK 306
             ++LY                 SS ++    +DG+   L        EE     +   K
Sbjct: 66  ESRFLYPGKNGRLGDFFGDPGNPSSEFTPSGSKDGKYELLTAANEVIAEEI---KDLMTK 125

Query: 307 EDPREGTMSSLLSGSLSMALCCI 309
            D       +LL+GSL+ ALC I
Sbjct: 126 SDIEGQHTETLLAGSLAKALCYI 145

[Close]

BLAST of CmaCh15G006840 vs. ExPASy TrEMBL
Match: A0A6J1JZK7 (RNA polymerase II transcription factor B subunit 4 OS=Cucurbita maxima OX=3661 GN=LOC111489720 PE=3 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 2.4e-64
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = 0


Query: 177 MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 236
           MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI
Sbjct: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 60

Query: 237 GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIKEDPREGTMSSL 296
           GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIKEDPREGTMSSL
Sbjct: 61  GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIKEDPREGTMSSL 120

Query: 297 LSGSLSMALC 307
           LSGSLSMALC
Sbjct: 121 LSGSLSMALC 130

[Close]

BLAST of CmaCh15G006840 vs. ExPASy TrEMBL
Match: A0A6J1FJQ6 (RNA polymerase II transcription factor B subunit 4 OS=Cucurbita moschata OX=3662 GN=LOC111444867 PE=3 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 4.6e-63
Identity = 129/132 (97.73%), Postives = 130/132 (98.48%), Query Frame = 0


Query: 177 MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 236
           MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI
Sbjct: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 60

Query: 237 GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIKEDPREGTMSSL 296
           GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSI+EDPR GTMSSL
Sbjct: 61  GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIEEDPRGGTMSSL 120

Query: 297 LSGSLSMALCCI 309
           LSGSLSMALC I
Sbjct: 121 LSGSLSMALCYI 132

[Close]

BLAST of CmaCh15G006840 vs. ExPASy TrEMBL
Match: A0A5A7U2L2 (RNA polymerase II transcription factor B subunit 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold648G001190 PE=3 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 6.2e-60
Identity = 124/132 (93.94%), Postives = 126/132 (95.45%), Query Frame = 0


Query: 177 MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 236
           MASAPSKLYADDVSLLVVLLDTNPFFWST SLPFSKFLSHVLAFLNSILVLNQLNEVVVI
Sbjct: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 60

Query: 237 GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIKEDPREGTMSSL 296
           GTGYASCKYLYNSSSYSN  LEDGRMPALCTRLL NLEEF+IGDEQSIKEDP+ GTMSSL
Sbjct: 61  GTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSL 120

Query: 297 LSGSLSMALCCI 309
           LSGSLSMALC I
Sbjct: 121 LSGSLSMALCYI 132

[Close]

BLAST of CmaCh15G006840 vs. ExPASy TrEMBL
Match: A0A1S3B4H7 (LOW QUALITY PROTEIN: RNA polymerase II transcription factor B subunit 4 OS=Cucumis melo OX=3656 GN=LOC103485913 PE=3 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 6.2e-60
Identity = 124/132 (93.94%), Postives = 126/132 (95.45%), Query Frame = 0


Query: 177 MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 236
           MASAPSKLYADDVSLLVVLLDTNPFFWST SLPFSKFLSHVLAFLNSILVLNQLNEVVVI
Sbjct: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 60

Query: 237 GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIKEDPREGTMSSL 296
           GTGYASCKYLYNSSSYSN  LEDGRMPALCTRLL NLEEF+IGDEQSIKEDP+ GTMSSL
Sbjct: 61  GTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSL 120

Query: 297 LSGSLSMALCCI 309
           LSGSLSMALC I
Sbjct: 121 LSGSLSMALCYI 132

[Close]

BLAST of CmaCh15G006840 vs. ExPASy TrEMBL
Match: A0A0A0LMM2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G072450 PE=3 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 1.4e-59
Identity = 123/132 (93.18%), Postives = 126/132 (95.45%), Query Frame = 0


Query: 177 MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 236
           MASAPSKLYADDVSLLVVLLDTNPFFWST +LPFSKFLSHVLAFLNSILVLNQLNEVVVI
Sbjct: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSALPFSKFLSHVLAFLNSILVLNQLNEVVVI 60

Query: 237 GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIKEDPREGTMSSL 296
           GTGYASCKYLYNSSSYSN  LEDGRMPALCTRLL NLEEF+IGDEQSIKEDP+ GTMSSL
Sbjct: 61  GTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSL 120

Query: 297 LSGSLSMALCCI 309
           LSGSLSMALC I
Sbjct: 121 LSGSLSMALCYI 132

[Close]

BLAST of CmaCh15G006840 vs. NCBI nr
Match: KAG6578972.1 (LysM domain-containing GPI-anchored protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 290.0 bits (741), Expect = 2.4e-74
Identity = 156/170 (91.76%), Postives = 157/170 (92.35%), Query Frame = 0


Query: 139 VEEDQSDCKLGVSFCPRRRKSIRIRTGRLLSVANSETAMASAPSKLYADDVSLLVVLLDT 198
           VEE+Q D KL      R  KSIR RTGRLLSVANSETAMASAPSKLYADDVSLLVVLLDT
Sbjct: 520 VEEEQRDPKL------RGGKSIRSRTGRLLSVANSETAMASAPSKLYADDVSLLVVLLDT 579

Query: 199 NPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVIGTGYASCKYLYNSSSYSNRSLE 258
           NPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVIGTGYASCKYLYNSSSYSNRSLE
Sbjct: 580 NPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVIGTGYASCKYLYNSSSYSNRSLE 639

Query: 259 DGRMPALCTRLLNNLEEFMIGDEQSIKEDPREGTMSSLLSGSLSMALCCI 309
           DGRMPALCTRLLNNLEEFMIGDEQSIKEDPR GTMSSLLSGSLSMALC I
Sbjct: 640 DGRMPALCTRLLNNLEEFMIGDEQSIKEDPRGGTMSSLLSGSLSMALCYI 683

[Close]

BLAST of CmaCh15G006840 vs. NCBI nr
Match: XP_022993825.1 (RNA polymerase II transcription factor B subunit 4 [Cucurbita maxima])

HSP 1 Score: 255.8 bits (652), Expect = 5.0e-64
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = 0


Query: 177 MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 236
           MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI
Sbjct: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 60

Query: 237 GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIKEDPREGTMSSL 296
           GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIKEDPREGTMSSL
Sbjct: 61  GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIKEDPREGTMSSL 120

Query: 297 LSGSLSMALC 307
           LSGSLSMALC
Sbjct: 121 LSGSLSMALC 130

[Close]

BLAST of CmaCh15G006840 vs. NCBI nr
Match: XP_023550583.1 (RNA polymerase II transcription factor B subunit 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 253.1 bits (645), Expect = 3.2e-63
Identity = 130/132 (98.48%), Postives = 130/132 (98.48%), Query Frame = 0


Query: 177 MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 236
           MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI
Sbjct: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 60

Query: 237 GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIKEDPREGTMSSL 296
           GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIKEDPR GTMSSL
Sbjct: 61  GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIKEDPRGGTMSSL 120

Query: 297 LSGSLSMALCCI 309
           LSGSLSMALC I
Sbjct: 121 LSGSLSMALCYI 132

[Close]

BLAST of CmaCh15G006840 vs. NCBI nr
Match: XP_022938728.1 (RNA polymerase II transcription factor B subunit 4 [Cucurbita moschata])

HSP 1 Score: 251.5 bits (641), Expect = 9.4e-63
Identity = 129/132 (97.73%), Postives = 130/132 (98.48%), Query Frame = 0


Query: 177 MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 236
           MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI
Sbjct: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 60

Query: 237 GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIKEDPREGTMSSL 296
           GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSI+EDPR GTMSSL
Sbjct: 61  GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIEEDPRGGTMSSL 120

Query: 297 LSGSLSMALCCI 309
           LSGSLSMALC I
Sbjct: 121 LSGSLSMALCYI 132

[Close]

BLAST of CmaCh15G006840 vs. NCBI nr
Match: XP_008441918.2 (PREDICTED: LOW QUALITY PROTEIN: RNA polymerase II transcription factor B subunit 4 [Cucumis melo])

HSP 1 Score: 241.1 bits (614), Expect = 1.3e-59
Identity = 124/132 (93.94%), Postives = 126/132 (95.45%), Query Frame = 0


Query: 177 MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 236
           MASAPSKLYADDVSLLVVLLDTNPFFWST SLPFSKFLSHVLAFLNSILVLNQLNEVVVI
Sbjct: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 60

Query: 237 GTGYASCKYLYNSSSYSNRSLEDGRMPALCTRLLNNLEEFMIGDEQSIKEDPREGTMSSL 296
           GTGYASCKYLYNSSSYSN  LEDGRMPALCTRLL NLEEF+IGDEQSIKEDP+ GTMSSL
Sbjct: 61  GTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSL 120

Query: 297 LSGSLSMALCCI 309
           LSGSLSMALC I
Sbjct: 121 LSGSLSMALCYI 132

[Close]

BLAST of CmaCh15G006840 vs. TAIR 10
Match: AT1G18340.1 (basal transcription factor complex subunit-related )

HSP 1 Score: 144.4 bits (363), Expect = 1.5e-34
Identity = 82/136 (60.29%), Postives = 99/136 (72.79%), Query Frame = 0


Query: 177 MASAPSKLYADDVSLLVVLLDTNPFFWSTYSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 236
           M +  SK Y+DDVSLLV+LLDTNP FWST S+ FS+FLSHVLAFLN++L LNQLN+VVVI
Sbjct: 1   MPAIASKQYSDDVSLLVLLLDTNPLFWSTTSITFSQFLSHVLAFLNAVLGLNQLNQVVVI 60

Query: 237 GTGYASCKYLYNSSSYSNRSLEDGR---MPALCTRLLNNLEEFMIGDEQSIKEDPREGTM 296
            TGY+SC Y+Y+SS  SN    +     MPA+   LL  LEEF+  DE+  KE+  E  +
Sbjct: 61  ATGYSSCDYIYDSSLTSNHGNFESNGTGMPAIFGSLLKKLEEFVTKDEELSKEEVSEDRI 120

Query: 297 -SSLLSGSLSMALCCI 309
            S LLSGSLSMALC I
Sbjct: 121 PSCLLSGSLSMALCYI 136

The following BLAST results are available for this feature:

BLAST of CmaCh15G006840 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LF41	2.1e-33	60.29	General transcription and DNA repair factor IIH subunit TFB4 OS=Arabidopsis thal...	[more]
O74366	3.8e-06	30.40	General transcription and DNA repair factor IIH subunit tfb4 OS=Schizosaccharomy...	[more]
Q13889	1.2e-04	26.39	General transcription factor IIH subunit 3 OS=Homo sapiens OX=9606 GN=GTF2H3 PE=...	[more]
Q05B56	1.6e-04	27.97	General transcription factor IIH subunit 3 OS=Bos taurus OX=9913 GN=GTF2H3 PE=2 ...	[more]

BLAST of CmaCh15G006840 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JZK7	2.4e-64	100.00	RNA polymerase II transcription factor B subunit 4 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1FJQ6	4.6e-63	97.73	RNA polymerase II transcription factor B subunit 4 OS=Cucurbita moschata OX=3662...	[more]
A0A5A7U2L2	6.2e-60	93.94	RNA polymerase II transcription factor B subunit 4 OS=Cucumis melo var. makuwa O...	[more]
A0A1S3B4H7	6.2e-60	93.94	LOW QUALITY PROTEIN: RNA polymerase II transcription factor B subunit 4 OS=Cucum...	[more]
A0A0A0LMM2	1.4e-59	93.18	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G072450 PE=3 SV=1	[more]

BLAST of CmaCh15G006840 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6578972.1	2.4e-74	91.76	LysM domain-containing GPI-anchored protein 1, partial [Cucurbita argyrosperma s...	[more]
XP_022993825.1	5.0e-64	100.00	RNA polymerase II transcription factor B subunit 4 [Cucurbita maxima]	[more]
XP_023550583.1	3.2e-63	98.48	RNA polymerase II transcription factor B subunit 4 [Cucurbita pepo subsp. pepo]	[more]
XP_022938728.1	9.4e-63	97.73	RNA polymerase II transcription factor B subunit 4 [Cucurbita moschata]	[more]
XP_008441918.2	1.3e-59	93.94	PREDICTED: LOW QUALITY PROTEIN: RNA polymerase II transcription factor B subunit...	[more]

BLAST of CmaCh15G006840 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18340.1	1.5e-34	60.29	basal transcription factor complex subunit-related	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004600	TFIIH subunit Tfb4/GTF2H3	PFAM	PF03850	Tfb4	coord: 189..308 e-value: 3.3E-19 score: 69.4
IPR004600	TFIIH subunit Tfb4/GTF2H3	PANTHER	PTHR12831	TRANSCRIPTION INITIATION FACTOR IIH TFIIH , POLYPEPTIDE 3-RELATED	coord: 177..308
IPR036465	von Willebrand factor A-like domain superfamily	GENE3D	3.40.50.410	von Willebrand factor, type A domain	coord: 186..310 e-value: 9.0E-23 score: 83.0

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh15G006840.1	CmaCh15G006840.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006289	nucleotide-excision repair
biological_process	GO:0070816	phosphorylation of RNA polymerase II C-terminal domain
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0000439	transcription factor TFIIH core complex
cellular_component	GO:0005675	transcription factor TFIIH holo complex
molecular_function	GO:0046872	metal ion binding