CmaCh15G006740 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh15G006740
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	E3 ubiquitin-protein ligase RING1-like
Location	Cma_Chr15: 3232077 .. 3232757 (+)
RNA-Seq Expression	CmaCh15G006740
Synteny	CmaCh15G006740

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATGGATACTCTCTACGAATACCAGATATGGCAACCTGAAGAAGGTGAAGATGATATTAATTTTGCCATTTCCAATTCTCACTTGCTTCCCTGTGACTGTTTCCTTTTCCACCTTCGCTTTCGCCATGTTCTTCAGTCAAATCACTCTCAAGAATTTCACACTCTCCTAAATTCCTCCCTATCTTCGTCCTTGATACCTTGCGATGTCTTTCTCGCCAGTGGCTCCAGTATCCTGATTGCTGTTTTCTCCGGCACTGGCGTCGCGCAAGACTTCTTGGACGCGGCAGTACCGGACGTTCTGTCCGTCGCGGCTGATATCGTTTGCAATCCTCTTAATGCTGGCCGTCGGATATTTCCGTTGGCGGTGTCTGTTTTAGTCGTTTCGCTGTACAATGAACGCGAGGAAATTGGGCGAGTTTTGCGGGAATCATCAGAGCAAGCGTTTAAAGCGTCGCCGGCGTGTGCGTCGGCTATCGAAGGTTTGAAGAACTTGGAGATTGATGTCCATGGAGAGTTGTTGATCGGAGAATGCAGAATATGTTTGGATGAATTGATGAACGGAATGGAAGTTACGCAATTACCGTGCGCTCATTTTTACCACAGAGAGTGTATCGTCAAATGGCTAGAGACGAGTCATTTGTGCCCGTTGTGCCGCTACGCAATGCCATTGAGCTGA

mRNA sequence

ATGGCCATGGATACTCTCTACGAATACCAGATATGGCAACCTGAAGAAGGTGAAGATGATATTAATTTTGCCATTTCCAATTCTCACTTGCTTCCCTGTGACTGTTTCCTTTTCCACCTTCGCTTTCGCCATGTTCTTCAGTCAAATCACTCTCAAGAATTTCACACTCTCCTAAATTCCTCCCTATCTTCGTCCTTGATACCTTGCGATGTCTTTCTCGCCAGTGGCTCCAGTATCCTGATTGCTGTTTTCTCCGGCACTGGCGTCGCGCAAGACTTCTTGGACGCGGCAGTACCGGACGTTCTGTCCGTCGCGGCTGATATCGTTTGCAATCCTCTTAATGCTGGCCGTCGGATATTTCCGTTGGCGGTGTCTGTTTTAGTCGTTTCGCTGTACAATGAACGCGAGGAAATTGGGCGAGTTTTGCGGGAATCATCAGAGCAAGCGTTTAAAGCGTCGCCGGCGTGTGCGTCGGCTATCGAAGGTTTGAAGAACTTGGAGATTGATGTCCATGGAGAGTTGTTGATCGGAGAATGCAGAATATGTTTGGATGAATTGATGAACGGAATGGAAGTTACGCAATTACCGTGCGCTCATTTTTACCACAGAGAGTGTATCGTCAAATGGCTAGAGACGAGTCATTTGTGCCCGTTGTGCCGCTACGCAATGCCATTGAGCTGA

Coding sequence (CDS)

ATGGCCATGGATACTCTCTACGAATACCAGATATGGCAACCTGAAGAAGGTGAAGATGATATTAATTTTGCCATTTCCAATTCTCACTTGCTTCCCTGTGACTGTTTCCTTTTCCACCTTCGCTTTCGCCATGTTCTTCAGTCAAATCACTCTCAAGAATTTCACACTCTCCTAAATTCCTCCCTATCTTCGTCCTTGATACCTTGCGATGTCTTTCTCGCCAGTGGCTCCAGTATCCTGATTGCTGTTTTCTCCGGCACTGGCGTCGCGCAAGACTTCTTGGACGCGGCAGTACCGGACGTTCTGTCCGTCGCGGCTGATATCGTTTGCAATCCTCTTAATGCTGGCCGTCGGATATTTCCGTTGGCGGTGTCTGTTTTAGTCGTTTCGCTGTACAATGAACGCGAGGAAATTGGGCGAGTTTTGCGGGAATCATCAGAGCAAGCGTTTAAAGCGTCGCCGGCGTGTGCGTCGGCTATCGAAGGTTTGAAGAACTTGGAGATTGATGTCCATGGAGAGTTGTTGATCGGAGAATGCAGAATATGTTTGGATGAATTGATGAACGGAATGGAAGTTACGCAATTACCGTGCGCTCATTTTTACCACAGAGAGTGTATCGTCAAATGGCTAGAGACGAGTCATTTGTGCCCGTTGTGCCGCTACGCAATGCCATTGAGCTGA

Protein sequence

MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNSSLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIVCNPLNAGRRIFPLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECRICLDELMNGMEVTQLPCAHFYHRECIVKWLETSHLCPLCRYAMPLS

Homology

[Close]

BLAST of CmaCh15G006740 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.5e-12
Identity = 32/92 (34.78%), Postives = 54/92 (58.70%), Query Frame = 0


Query: 136 EEIGRVLRESSEQAFKASPACASAIEGLKNLEI---DVHGELLIGECRICLDELMNGMEV 195
           E++ + L E+    +   PA  SAIE L  + I   +++ E    +C +C+D+   G E 
Sbjct: 180 EQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSE--FNQCAVCMDDFEEGTEA 239

Query: 196 TQLPCAHFYHRECIVKWLETSHLCPLCRYAMP 225
            Q+PC H YH++C++ WLE  + CP+CR+ +P
Sbjct: 240 KQMPCKHLYHKDCLLPWLELHNSCPVCRHELP 269

[Close]

BLAST of CmaCh15G006740 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.5e-12
Identity = 31/92 (33.70%), Postives = 56/92 (60.87%), Query Frame = 0


Query: 136 EEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELL---IGECRICLDELMNGMEV 195
           E++ + L E+    +   PA  SAI+ L    + V  ++L   + +C +C+DE  +G +V
Sbjct: 172 EQLIQQLAENDPNRYGTPPASKSAIDALPT--VKVTKDMLKSEMNQCAVCMDEFEDGSDV 231

Query: 196 TQLPCAHFYHRECIVKWLETSHLCPLCRYAMP 225
            Q+PC H +H++C++ WLE  + CP+CR+ +P
Sbjct: 232 KQMPCKHVFHQDCLLPWLELHNSCPVCRFELP 261

[Close]

BLAST of CmaCh15G006740 vs. ExPASy Swiss-Prot
Match: Q9SPL2 (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.1e-10
Identity = 29/72 (40.28%), Postives = 42/72 (58.33%), Query Frame = 0


Query: 152 ASPACASAIEGLKNLEIDVHGELLIGECRICLDELMNGMEVTQLPCAHFYHRECIVKWLE 211
           A PA  SAIE L+  E+      ++  C +C D ++ G    +LPC H YH +CIV WL 
Sbjct: 230 APPAAKSAIEALETFEVSSSEGEMVMVCAVCKDGMVMGETGKKLPCGHCYHGDCIVPWLG 289

Query: 212 TSHLCPLCRYAM 224
           T + CP+CR+ +
Sbjct: 290 TRNSCPVCRFQL 301

[Close]

BLAST of CmaCh15G006740 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 4.0e-10
Identity = 38/115 (33.04%), Postives = 60/115 (52.17%), Query Frame = 0


Query: 115 AGRRIFPLAVSVLVVSLYNER--EEIGRVLRESSEQAFKAS---PACASAIEGLKNLEID 174
           +G R  P +VS +++    ER  E++ ++  E+S      S   PA  SAIE L  +EI 
Sbjct: 148 SGLRPLPDSVSEILMGSGFERLLEQLSQI--EASGNGIGRSGNPPASKSAIESLPRVEIS 207

Query: 175 VHGELLIGECRICLDELMNGMEVTQLPCAHFYHRECIVKWLETSHLCPLCRYAMP 225
                    C +C +    G+E  ++PC H +H +CIV WL   + CP+CR+ +P
Sbjct: 208 DCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELP 260

[Close]

BLAST of CmaCh15G006740 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 4.0e-10
Identity = 30/83 (36.14%), Postives = 44/83 (53.01%), Query Frame = 0


Query: 142 LRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECRICLDELMNGMEVTQLPCAHFY 201
           L ES        PA   A+E L  ++I+      +  C +CLD+L  G +  Q+PC H +
Sbjct: 167 LAESDPSRNGTPPAKKEAVEALPTVKIEE-----VVSCSVCLDDLEVGSQAKQMPCEHKF 226

Query: 202 HRECIVKWLETSHLCPLCRYAMP 225
           H  CI+ WLE    CP+CR+ +P
Sbjct: 227 HSSCILPWLELHSSCPVCRFELP 244

[Close]

BLAST of CmaCh15G006740 vs. ExPASy TrEMBL
Match: A0A6J1JV38 (uncharacterized protein LOC111489156 OS=Cucurbita maxima OX=3661 GN=LOC111489156 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 1.8e-125
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS
Sbjct: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIVCNPLNAGRRIF 120
           SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIVCNPLNAGRRIF
Sbjct: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIVCNPLNAGRRIF 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECR 180
           PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECR
Sbjct: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCAHFYHRECIVKWLETSHLCPLCRYAMPLS 227
           ICLDELMNGMEVTQLPCAHFYHRECIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTQLPCAHFYHRECIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of CmaCh15G006740 vs. ExPASy TrEMBL
Match: A0A6J1FKV9 (uncharacterized protein LOC111445148 OS=Cucurbita moschata OX=3662 GN=LOC111445148 PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 2.4e-122
Identity = 220/226 (97.35%), Postives = 224/226 (99.12%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS
Sbjct: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIVCNPLNAGRRIF 120
           SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVP+VLSVAAD+VCNPLNAGRRIF
Sbjct: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPEVLSVAADVVCNPLNAGRRIF 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECR 180
           PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKN+EIDV GELLIGECR
Sbjct: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCAHFYHRECIVKWLETSHLCPLCRYAMPLS 227
           ICLDELM+GMEVTQLPC HFYHRECIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMSGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of CmaCh15G006740 vs. ExPASy TrEMBL
Match: A0A6J1HJY0 (probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita moschata OX=3662 GN=LOC111463602 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 3.7e-107
Identity = 196/226 (86.73%), Postives = 202/226 (89.38%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLY+YQIWQPEE EDD+N  ISNSHLLPCDCFLFHL FRHVLQSNH QE   LLNS
Sbjct: 1   MAMDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIVCNPLNAGRRIF 120
           SLSS LIPCD F ASG SILIAVFS TGVAQDFLDAAVPDVLSVAADI CNPLNAGRR+ 
Sbjct: 61  SLSSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRVL 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECR 180
           PLAVSVL VS YNEREEIGRVLRESS Q FK SPA  SAIEGLK +EIDV GELLIGECR
Sbjct: 121 PLAVSVLAVSPYNEREEIGRVLRESSPQEFKTSPASESAIEGLKTVEIDVGGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCAHFYHRECIVKWLETSHLCPLCRYAMPLS 227
           ICLDELMNGMEVT+LPCAHFYHR+CIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTRLPCAHFYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of CmaCh15G006740 vs. ExPASy TrEMBL
Match: A0A6J1JSE1 (probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita maxima OX=3661 GN=LOC111487409 PE=4 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 4.8e-107
Identity = 196/226 (86.73%), Postives = 202/226 (89.38%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLY+YQIWQPEE EDD+N  ISNSHLLPCDCFLFHL FRHVLQSNH QE   LLNS
Sbjct: 1   MAMDTLYDYQIWQPEEHEDDVNVTISNSHLLPCDCFLFHLHFRHVLQSNHPQELQRLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIVCNPLNAGRRIF 120
           SLSS LIPCD F ASG SILIAVFS TGVAQDFLDAAVPDVLSVAADI CNPLNAGRR+ 
Sbjct: 61  SLSSFLIPCDAFFASGFSILIAVFSDTGVAQDFLDAAVPDVLSVAADIACNPLNAGRRVL 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECR 180
           PLAVSVL VS YNEREEIGRVLRESS QAFK SPA  SAIEGLK +EIDV GELLIGECR
Sbjct: 121 PLAVSVLAVSPYNEREEIGRVLRESSPQAFKTSPASESAIEGLKTVEIDVGGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCAHFYHRECIVKWLETSHLCPLCRYAMPLS 227
           ICLDELMNGMEVT+LPCAH YHR+CIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of CmaCh15G006740 vs. ExPASy TrEMBL
Match: A0A0A0KDU3 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G385070 PE=4 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 4.8e-107
Identity = 194/226 (85.84%), Postives = 204/226 (90.27%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLY+Y IWQPEEGEDD+N  ISNSHLLPC+CFLFHL FRH+LQSNHSQ+FH LLNS
Sbjct: 1   MAMDTLYDYHIWQPEEGEDDLNVTISNSHLLPCNCFLFHLHFRHILQSNHSQQFHYLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIVCNPLNAGRRIF 120
           SLSS LIPCDVF A+ SSILIAVFSGTGVAQDFLD AVPDVLSVAADI CNPLNAGR I 
Sbjct: 61  SLSSFLIPCDVFFATASSILIAVFSGTGVAQDFLDTAVPDVLSVAADIACNPLNAGRTIL 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECR 180
           PLAVSVLVVS YNER+EIGRVLRESS QAFK  PA   AIEGLK +EIDV GELLIGECR
Sbjct: 121 PLAVSVLVVSPYNERQEIGRVLRESSPQAFKTLPASEMAIEGLKKVEIDVGGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCAHFYHRECIVKWLETSHLCPLCRYAMPLS 227
           ICLDELMNGMEVT+LPCAH YHR+CIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTRLPCAHLYHRDCIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of CmaCh15G006740 vs. NCBI nr
Match: XP_022992996.1 (uncharacterized protein LOC111489156 [Cucurbita maxima])

HSP 1 Score: 458.4 bits (1178), Expect = 3.6e-125
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS
Sbjct: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIVCNPLNAGRRIF 120
           SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIVCNPLNAGRRIF
Sbjct: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIVCNPLNAGRRIF 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECR 180
           PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECR
Sbjct: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCAHFYHRECIVKWLETSHLCPLCRYAMPLS 227
           ICLDELMNGMEVTQLPCAHFYHRECIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTQLPCAHFYHRECIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of CmaCh15G006740 vs. NCBI nr
Match: KAG7016485.1 (E3 ubiquitin-protein ligase RING1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 449.9 bits (1156), Expect = 1.3e-122
Identity = 221/226 (97.79%), Postives = 224/226 (99.12%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS
Sbjct: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIVCNPLNAGRRIF 120
           SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVP+VLSVAAD+VCNPLNAGRRIF
Sbjct: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPEVLSVAADVVCNPLNAGRRIF 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECR 180
           PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKN+EIDV GELLIGECR
Sbjct: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCAHFYHRECIVKWLETSHLCPLCRYAMPLS 227
           ICLDELMNGMEVTQLPC HFYHRECIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of CmaCh15G006740 vs. NCBI nr
Match: XP_022939153.1 (uncharacterized protein LOC111445148 [Cucurbita moschata])

HSP 1 Score: 448.0 bits (1151), Expect = 4.9e-122
Identity = 220/226 (97.35%), Postives = 224/226 (99.12%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS
Sbjct: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIVCNPLNAGRRIF 120
           SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVP+VLSVAAD+VCNPLNAGRRIF
Sbjct: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPEVLSVAADVVCNPLNAGRRIF 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECR 180
           PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKN+EIDV GELLIGECR
Sbjct: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCAHFYHRECIVKWLETSHLCPLCRYAMPLS 227
           ICLDELM+GMEVTQLPC HFYHRECIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMSGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of CmaCh15G006740 vs. NCBI nr
Match: KAG6578963.1 (E3 ubiquitin-protein ligase RING1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 446.8 bits (1148), Expect = 1.1e-121
Identity = 220/226 (97.35%), Postives = 223/226 (98.67%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLYEYQIWQPEEGEDDI FAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS
Sbjct: 1   MAMDTLYEYQIWQPEEGEDDIYFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIVCNPLNAGRRIF 120
           SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVP+VLSVAAD+VCNPLNAGRRIF
Sbjct: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPEVLSVAADVVCNPLNAGRRIF 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECR 180
           PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKN+EIDV GELLIGECR
Sbjct: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCAHFYHRECIVKWLETSHLCPLCRYAMPLS 227
           ICLDELMNGMEVTQLPC HFYHRECIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of CmaCh15G006740 vs. NCBI nr
Match: XP_023550658.1 (uncharacterized protein LOC111808739 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 445.3 bits (1144), Expect = 3.2e-121
Identity = 220/226 (97.35%), Postives = 222/226 (98.23%), Query Frame = 0


Query: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60
           MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS
Sbjct: 1   MAMDTLYEYQIWQPEEGEDDINFAISNSHLLPCDCFLFHLRFRHVLQSNHSQEFHTLLNS 60

Query: 61  SLSSSLIPCDVFLASGSSILIAVFSGTGVAQDFLDAAVPDVLSVAADIVCNPLNAGRRIF 120
           SLSSSLIPCDVFLASGSSILIAVFS TGVAQDFLDAAVP+VLSVAADIVCNPLN GRRIF
Sbjct: 61  SLSSSLIPCDVFLASGSSILIAVFSDTGVAQDFLDAAVPEVLSVAADIVCNPLNDGRRIF 120

Query: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECR 180
           PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKN+EIDV GELLIGECR
Sbjct: 121 PLAVSVLVVSLYNEREEIGRVLRESSEQAFKASPACASAIEGLKNVEIDVRGELLIGECR 180

Query: 181 ICLDELMNGMEVTQLPCAHFYHRECIVKWLETSHLCPLCRYAMPLS 227
           ICLDELMNGMEVTQLPC HFYHRECIVKWLETSHLCPLCRYAMPLS
Sbjct: 181 ICLDELMNGMEVTQLPCDHFYHRECIVKWLETSHLCPLCRYAMPLS 226

[Close]

BLAST of CmaCh15G006740 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 73.9 bits (180), Expect = 1.8e-13
Identity = 31/92 (33.70%), Postives = 56/92 (60.87%), Query Frame = 0


Query: 136 EEIGRVLRESSEQAFKASPACASAIEGLKNLEIDVHGELL---IGECRICLDELMNGMEV 195
           E++ + L E+    +   PA  SAI+ L    + V  ++L   + +C +C+DE  +G +V
Sbjct: 172 EQLIQQLAENDPNRYGTPPASKSAIDALPT--VKVTKDMLKSEMNQCAVCMDEFEDGSDV 231

Query: 196 TQLPCAHFYHRECIVKWLETSHLCPLCRYAMP 225
            Q+PC H +H++C++ WLE  + CP+CR+ +P
Sbjct: 232 KQMPCKHVFHQDCLLPWLELHNSCPVCRFELP 261

[Close]

BLAST of CmaCh15G006740 vs. TAIR 10
Match: AT3G02340.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.1 bits (170), Expect = 2.6e-12
Identity = 33/73 (45.21%), Postives = 46/73 (63.01%), Query Frame = 0


Query: 154 PACASAIEGLKNLEIDVHGELLIGE--CRICLDELMNGMEVTQLPCAHFYHRECIVKWLE 213
           PA  S I+ L  +E+ V  EL  G   C +C DE++   +V +LPC+HFYH ECI+ WL 
Sbjct: 309 PAAKSVIQDLPVVELAVE-ELDKGNNVCAVCKDEMLVEEKVRRLPCSHFYHGECIIPWLG 368

Query: 214 TSHLCPLCRYAMP 225
             + CP+CRY +P
Sbjct: 369 IRNTCPVCRYELP 380

[Close]

BLAST of CmaCh15G006740 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 68.6 bits (166), Expect = 7.6e-12
Identity = 30/85 (35.29%), Postives = 47/85 (55.29%), Query Frame = 0


Query: 140 RVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECRICLDELMNGMEVTQLPCAH 199
           R+        +   PA   A+E L  ++I+    LL  +C +CLD+   GME  ++PC H
Sbjct: 190 RLAENDLNNRYGTPPATKEAVEALAMVKIE--DSLL--QCSVCLDDFEIGMEAKEMPCKH 249

Query: 200 FYHRECIVKWLETSHLCPLCRYAMP 225
            +H +C++ WLE    CP+CRY +P
Sbjct: 250 KFHSDCLLPWLELHSSCPVCRYLLP 270

[Close]

BLAST of CmaCh15G006740 vs. TAIR 10
Match: AT3G13430.2 (RING/U-box superfamily protein )

HSP 1 Score: 68.6 bits (166), Expect = 7.6e-12
Identity = 30/85 (35.29%), Postives = 47/85 (55.29%), Query Frame = 0


Query: 140 RVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECRICLDELMNGMEVTQLPCAH 199
           R+        +   PA   A+E L  ++I+    LL  +C +CLD+   GME  ++PC H
Sbjct: 190 RLAENDLNNRYGTPPATKEAVEALAMVKIE--DSLL--QCSVCLDDFEIGMEAKEMPCKH 249

Query: 200 FYHRECIVKWLETSHLCPLCRYAMP 225
            +H +C++ WLE    CP+CRY +P
Sbjct: 250 KFHSDCLLPWLELHSSCPVCRYLLP 270

[Close]

BLAST of CmaCh15G006740 vs. TAIR 10
Match: AT3G13430.3 (RING/U-box superfamily protein )

HSP 1 Score: 68.6 bits (166), Expect = 7.6e-12
Identity = 30/85 (35.29%), Postives = 47/85 (55.29%), Query Frame = 0


Query: 140 RVLRESSEQAFKASPACASAIEGLKNLEIDVHGELLIGECRICLDELMNGMEVTQLPCAH 199
           R+        +   PA   A+E L  ++I+    LL  +C +CLD+   GME  ++PC H
Sbjct: 190 RLAENDLNNRYGTPPATKEAVEALAMVKIE--DSLL--QCSVCLDDFEIGMEAKEMPCKH 249

Query: 200 FYHRECIVKWLETSHLCPLCRYAMP 225
            +H +C++ WLE    CP+CRY +P
Sbjct: 250 KFHSDCLLPWLELHSSCPVCRYLLP 270

The following BLAST results are available for this feature:

BLAST of CmaCh15G006740 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0CH30	1.5e-12	34.78	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q8LPN7	2.5e-12	33.70	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q9SPL2	1.1e-10	40.28	E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV...	[more]
Q9SNB6	4.0e-10	33.04	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
Q6AVN2	4.0e-10	36.14	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]

BLAST of CmaCh15G006740 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JV38	1.8e-125	100.00	uncharacterized protein LOC111489156 OS=Cucurbita maxima OX=3661 GN=LOC111489156...	[more]
A0A6J1FKV9	2.4e-122	97.35	uncharacterized protein LOC111445148 OS=Cucurbita moschata OX=3662 GN=LOC1114451...	[more]
A0A6J1HJY0	3.7e-107	86.73	probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1JSE1	4.8e-107	86.73	probable E3 ubiquitin-protein ligase ATL45 OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A0A0KDU3	4.8e-107	85.84	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G385070 P...	[more]

BLAST of CmaCh15G006740 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022992996.1	3.6e-125	100.00	uncharacterized protein LOC111489156 [Cucurbita maxima]	[more]
KAG7016485.1	1.3e-122	97.79	E3 ubiquitin-protein ligase RING1-like protein, partial [Cucurbita argyrosperma ...	[more]
XP_022939153.1	4.9e-122	97.35	uncharacterized protein LOC111445148 [Cucurbita moschata]	[more]
KAG6578963.1	1.1e-121	97.35	E3 ubiquitin-protein ligase RING1-like protein, partial [Cucurbita argyrosperma ...	[more]
XP_023550658.1	3.2e-121	97.35	uncharacterized protein LOC111808739 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmaCh15G006740 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19950.1	1.8e-13	33.70	RING/U-box superfamily protein	[more]
AT3G02340.1	2.6e-12	45.21	RING/U-box superfamily protein	[more]
AT3G13430.1	7.6e-12	35.29	RING/U-box superfamily protein	[more]
AT3G13430.2	7.6e-12	35.29	RING/U-box superfamily protein	[more]
AT3G13430.3	7.6e-12	35.29	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 178..220 e-value: 9.0E-4 score: 16.2
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 179..219 e-value: 7.0E-7 score: 38.9
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 178..220 e-value: 1.0E-13 score: 51.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 179..220 score: 12.931868
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 141..225 e-value: 2.2E-18 score: 67.7
None	No IPR available	PANTHER	PTHR15710:SF120	F6A14.12 PROTEIN-RELATED	coord: 93..224
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 93..224
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 167..224

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh15G006740.1	CmaCh15G006740.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding