CmaCh15G006640 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh15G006640
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionE3 ubiquitin ligase BIG BROTHER-related-like
LocationCma_Chr15: 3161979 .. 3162590 (+)
RNA-Seq ExpressionCmaCh15G006640
SyntenyCmaCh15G006640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAGAGCAAGAGGATAAGCAGCAGCTCCCCCAAAGGATTTCCCTTGGTTATGTGGAACAAGTGAACTCAGATTTGATCATGGCCTTGGCAATGCAGCAGCAGGAGCATGAGATGGCCTATACGACGCTTGGGACCATCGAAAGTGATAGCGACGAAGACGAAAGGTCTGATTCCAATAGCAACAATGGTCTCGACACCAATGCCTCCTTGCAAAGCCAATTTCTTGCAGATAATGAGGAGACTACTGATGAAAACGAAGATATGGACGAAGATGACGAGGAGGGATTTGAGGATTTTGATTTCGATGAACTAACCTATGAAGAGTTGATTGCATTAGGGGAATTCATTGGAGAAGAAAAGAGAGGGTTACCGTCGAACGAAATACCTTCATGCTTGCACTCGAGCAAGTTTCGAAGCATCGAAAATACAAGTGGTATAGATCGGTGTGTGATTTGCCAAGTTGAATATGAGGATGGTGAAGAATTGGCTGCCCTTCCTTGTGAGCATCCTTACCATTCAGACTGTATAGTAAAATGGGTTCAAACCAAAAGGGTTTGTCCAATATGTGGCACAGAAGTTTCATCCCCCAAGGCTTCTAAAAATGCATAA

mRNA sequence

ATGGGAGAGCAAGAGGATAAGCAGCAGCTCCCCCAAAGGATTTCCCTTGGTTATGTGGAACAAGTGAACTCAGATTTGATCATGGCCTTGGCAATGCAGCAGCAGGAGCATGAGATGGCCTATACGACGCTTGGGACCATCGAAAGTGATAGCGACGAAGACGAAAGGTCTGATTCCAATAGCAACAATGGTCTCGACACCAATGCCTCCTTGCAAAGCCAATTTCTTGCAGATAATGAGGAGACTACTGATGAAAACGAAGATATGGACGAAGATGACGAGGAGGGATTTGAGGATTTTGATTTCGATGAACTAACCTATGAAGAGTTGATTGCATTAGGGGAATTCATTGGAGAAGAAAAGAGAGGGTTACCGTCGAACGAAATACCTTCATGCTTGCACTCGAGCAAGTTTCGAAGCATCGAAAATACAAGTGGTATAGATCGGTGTGTGATTTGCCAAGTTGAATATGAGGATGGTGAAGAATTGGCTGCCCTTCCTTGTGAGCATCCTTACCATTCAGACTGTATAGTAAAATGGGTTCAAACCAAAAGGGTTTGTCCAATATGTGGCACAGAAGTTTCATCCCCCAAGGCTTCTAAAAATGCATAA

Coding sequence (CDS)

ATGGGAGAGCAAGAGGATAAGCAGCAGCTCCCCCAAAGGATTTCCCTTGGTTATGTGGAACAAGTGAACTCAGATTTGATCATGGCCTTGGCAATGCAGCAGCAGGAGCATGAGATGGCCTATACGACGCTTGGGACCATCGAAAGTGATAGCGACGAAGACGAAAGGTCTGATTCCAATAGCAACAATGGTCTCGACACCAATGCCTCCTTGCAAAGCCAATTTCTTGCAGATAATGAGGAGACTACTGATGAAAACGAAGATATGGACGAAGATGACGAGGAGGGATTTGAGGATTTTGATTTCGATGAACTAACCTATGAAGAGTTGATTGCATTAGGGGAATTCATTGGAGAAGAAAAGAGAGGGTTACCGTCGAACGAAATACCTTCATGCTTGCACTCGAGCAAGTTTCGAAGCATCGAAAATACAAGTGGTATAGATCGGTGTGTGATTTGCCAAGTTGAATATGAGGATGGTGAAGAATTGGCTGCCCTTCCTTGTGAGCATCCTTACCATTCAGACTGTATAGTAAAATGGGTTCAAACCAAAAGGGTTTGTCCAATATGTGGCACAGAAGTTTCATCCCCCAAGGCTTCTAAAAATGCATAA

Protein sequence

MGEQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDSDEDERSDSNSNNGLDTNASLQSQFLADNEETTDENEDMDEDDEEGFEDFDFDELTYEELIALGEFIGEEKRGLPSNEIPSCLHSSKFRSIENTSGIDRCVICQVEYEDGEELAALPCEHPYHSDCIVKWVQTKRVCPICGTEVSSPKASKNA
Homology
BLAST of CmaCh15G006640 vs. ExPASy Swiss-Prot
Match: Q9LT17 (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.9e-20
Identity = 58/170 (34.12%), Postives = 97/170 (57.06%), Query Frame = 0

Query: 48  ESDSDEDERSDSNSNNGLDTNASLQSQFLADNEE--------------TTDENEDMDEDD 107
           E D ++D  SD       D  A  ++   A+  +                ++ ED     
Sbjct: 172 EDDQEDDGNSDIEEVAYTDDEAYARALQEAEERDMAARLSALSGLANRVVEDLEDESHTS 231

Query: 108 EEGFEDFDFDELTYEELIALGEFIGEEKRGLPSNEIPSCLHSSKFRSIENTSGI-DRCVI 167
           ++ +++ D DEL+YEEL+ALG+ +G E RGL ++ I S L S +++  +N +G  + CVI
Sbjct: 232 QDAWDEMDPDELSYEELLALGDIVGTESRGLSADTIAS-LPSKRYKEGDNQNGTNESCVI 291

Query: 168 CQVEYEDGEELAALPCEHPYHSDCIVKWVQTKRVCPICGTEVSSPKASKN 203
           C+++YED E+L  LPC+H YHS+CI  W++  +VCP+C  EVS+  + ++
Sbjct: 292 CRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEVSTSTSGQS 340

BLAST of CmaCh15G006640 vs. ExPASy Swiss-Prot
Match: Q8L649 (E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 4.7e-18
Identity = 50/121 (41.32%), Postives = 70/121 (57.85%), Query Frame = 0

Query: 77  ADNEETTDENEDMDEDDEEGFEDFDFDELTYEELIALGEFIGEEKRGLPSNEIPSCLHSS 136
           ++ EE      D D       +D D D +TYEEL+ LGE +G E RGL S E+   L + 
Sbjct: 122 SEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGL-SQELIETLPTK 181

Query: 137 KFR---SIENTSGIDRCVICQVEYEDGEELAALPCEHPYHSDCIVKWVQTKRVCPICGTE 195
           K++           +RCVICQ++Y+ GE    LPC+H YHS+CI KW+   +VCP+C +E
Sbjct: 182 KYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSE 241

BLAST of CmaCh15G006640 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.1e-17
Identity = 44/98 (44.90%), Postives = 56/98 (57.14%), Query Frame = 0

Query: 101 DFDELTYEELIALGEFIGEEKRGLPSNEIPSCLH-----SSKFRSIENTSGIDRCVICQV 160
           D D ++YEEL+ALGE IG+   GL    I   +      SS   S+E    I+ C ICQ 
Sbjct: 602 DVDNMSYEELLALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIEPCCICQE 661

Query: 161 EYEDGEELAALPCEHPYHSDCIVKWVQTKRVCPICGTE 194
           EY +G+ L  L C H +H DCI +WV  K +CPIC TE
Sbjct: 662 EYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

BLAST of CmaCh15G006640 vs. ExPASy Swiss-Prot
Match: Q5QLR5 (Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=ZFP1 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.2e-15
Identity = 46/113 (40.71%), Postives = 60/113 (53.10%), Query Frame = 0

Query: 93  DEEGFEDFDFDELTYEELIALGEFIGEEKRGLPSNEIPSCLHSSKF---------RSIEN 152
           DE      D D +TYEEL+AL E IG+   GL  + I   L +S F         +S E+
Sbjct: 405 DEHRDMRLDIDSMTYEELLALEEQIGDVNTGLAKSYIVEKLKTSLFVPGSSCMSNKSSES 464

Query: 153 TSGIDRCVICQVEYEDGEELAALPCEHPYHSDCIVKWVQTKRVCPICGTEVSS 197
           +   D C+ICQ EY+  E +  L C H YH DCI +W+  K +CPIC T   S
Sbjct: 465 SMENDACIICQEEYQVKECIGTLDCGHRYHEDCIKQWLMVKNLCPICKTTALS 517

BLAST of CmaCh15G006640 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 83.6 bits (205), Expect = 2.9e-15
Identity = 39/92 (42.39%), Postives = 52/92 (56.52%), Query Frame = 0

Query: 101 DFDELTYEELIALGEFIGEEKRGLPSNEIPSCLHSSKFRS--IENTSGIDRCVICQVEYE 160
           D D ++YEEL+AL E IG    GL   E+   L   KF S  +E +   + C ICQ EY 
Sbjct: 569 DIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRKFSSWRLEASVEEEPCCICQEEYV 628

Query: 161 DGEELAALPCEHPYHSDCIVKWVQTKRVCPIC 191
           DG++L  L C H +H  C+ +W+  K  CPIC
Sbjct: 629 DGDDLGTLDCGHDFHVGCVRQWLVVKNTCPIC 660

BLAST of CmaCh15G006640 vs. ExPASy TrEMBL
Match: A0A6J1K3Q9 (E3 ubiquitin ligase BIG BROTHER-related-like OS=Cucurbita maxima OX=3661 GN=LOC111489783 PE=4 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 2.5e-107
Identity = 203/203 (100.00%), Postives = 203/203 (100.00%), Query Frame = 0

Query: 1   MGEQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDSDEDERSDSN 60
           MGEQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDSDEDERSDSN
Sbjct: 1   MGEQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDSDEDERSDSN 60

Query: 61  SNNGLDTNASLQSQFLADNEETTDENEDMDEDDEEGFEDFDFDELTYEELIALGEFIGEE 120
           SNNGLDTNASLQSQFLADNEETTDENEDMDEDDEEGFEDFDFDELTYEELIALGEFIGEE
Sbjct: 61  SNNGLDTNASLQSQFLADNEETTDENEDMDEDDEEGFEDFDFDELTYEELIALGEFIGEE 120

Query: 121 KRGLPSNEIPSCLHSSKFRSIENTSGIDRCVICQVEYEDGEELAALPCEHPYHSDCIVKW 180
           KRGLPSNEIPSCLHSSKFRSIENTSGIDRCVICQVEYEDGEELAALPCEHPYHSDCIVKW
Sbjct: 121 KRGLPSNEIPSCLHSSKFRSIENTSGIDRCVICQVEYEDGEELAALPCEHPYHSDCIVKW 180

Query: 181 VQTKRVCPICGTEVSSPKASKNA 204
           VQTKRVCPICGTEVSSPKASKNA
Sbjct: 181 VQTKRVCPICGTEVSSPKASKNA 203

BLAST of CmaCh15G006640 vs. ExPASy TrEMBL
Match: A0A0A0KFS4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G382920 PE=4 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 3.7e-82
Identity = 162/207 (78.26%), Postives = 180/207 (86.96%), Query Frame = 0

Query: 3   EQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDSDEDERSDSNSN 62
           +Q+ + QLPQRISL YVEQVN+DLIMALAMQQQEHE+AYTTL TI SDS+EDE SDSNSN
Sbjct: 10  QQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHELAYTTLETIASDSEEDENSDSNSN 69

Query: 63  NGLDTNASLQSQ-------FLADNEETTDENEDMDEDDEEGFEDFDFDELTYEELIALGE 122
           NGLDTNA+ Q Q       FL D+EETTD N+DM+ED++  FE FD DELTYEELIALGE
Sbjct: 70  NGLDTNATSQRQELVSRWAFLEDDEETTDGNDDMEEDEDGDFEGFDLDELTYEELIALGE 129

Query: 123 FIGEEKRGLPSNEIPSCLHSSKFRSIENTSGIDRCVICQVEYEDGEELAALPCEHPYHSD 182
           FIGEEKRGLP NEIPSCLHSSKF++IEN SGIDRCVICQVEY+DGEELAALPCEHPYHS+
Sbjct: 130 FIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVICQVEYDDGEELAALPCEHPYHSE 189

Query: 183 CIVKWVQTKRVCPICGTEVSSPKASKN 203
           CI +W+Q KRVCPICGTEVSSP  SKN
Sbjct: 190 CIGEWLQIKRVCPICGTEVSSPNGSKN 216

BLAST of CmaCh15G006640 vs. ExPASy TrEMBL
Match: A0A6J1GTM7 (E3 ubiquitin ligase BIG BROTHER-related OS=Cucurbita moschata OX=3662 GN=LOC111457005 PE=4 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 4.1e-81
Identity = 170/212 (80.19%), Postives = 182/212 (85.85%), Query Frame = 0

Query: 1   MGEQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDSDEDERSDSN 60
           M EQEDK QLPQRIS  YVEQVN+D IMALAMQQQEHE  YT L TIESDS+EDERSDSN
Sbjct: 1   MEEQEDK-QLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSN 60

Query: 61  SNNGLD-TNASLQSQ-------FLADN-EETTDENEDMDEDDEEGFEDFDFDELTYEELI 120
           S+NGLD TN S QSQ       FLAD+ EETTD+NE MDED+EE  E+FD DEL+YEELI
Sbjct: 61  SSNGLDHTNDSFQSQELHSPWGFLADDEEETTDDNEYMDEDEEEDIEEFDLDELSYEELI 120

Query: 121 ALGEFIGEEKRGLPSNEIPSCLHSSKFRSIENTSGIDRCVICQVEYEDGEELAALPCEHP 180
           ALGEFIGEEKRGLP NEIPSCLHSSKF++IEN SGIDRCVICQVEYED EELAALPCEHP
Sbjct: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP 180

Query: 181 YHSDCIVKWVQTKRVCPICGTEVSSPKASKNA 204
           YHS+CI KW+Q KRVCPICGTEVSSPK SKNA
Sbjct: 181 YHSECISKWLQIKRVCPICGTEVSSPKPSKNA 211

BLAST of CmaCh15G006640 vs. ExPASy TrEMBL
Match: A0A6J1JR32 (E3 ubiquitin ligase BIG BROTHER-related-like OS=Cucurbita maxima OX=3661 GN=LOC111487582 PE=4 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 7.0e-81
Identity = 170/212 (80.19%), Postives = 183/212 (86.32%), Query Frame = 0

Query: 1   MGEQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDSDEDERSDSN 60
           M EQEDK QLPQRIS  YVEQVN+D IMALAMQQQEHE  YT L TIESDS+EDERSDSN
Sbjct: 1   MEEQEDK-QLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSN 60

Query: 61  SNNGLD-TNASLQSQ-------FLADN-EETTDENEDMDEDDEEGFEDFDFDELTYEELI 120
           S+NGLD TN SLQSQ       FLAD+ EETTD+NE MDED+EE  E+FD DEL+YEELI
Sbjct: 61  SSNGLDHTNDSLQSQELHSPWGFLADDEEETTDDNEYMDEDEEEDIEEFDLDELSYEELI 120

Query: 121 ALGEFIGEEKRGLPSNEIPSCLHSSKFRSIENTSGIDRCVICQVEYEDGEELAALPCEHP 180
           ALGEFIGEEKRGLP NEIPSCLHSSKF++IEN SGIDRCVICQVEYED EELAALPCEHP
Sbjct: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP 180

Query: 181 YHSDCIVKWVQTKRVCPICGTEVSSPKASKNA 204
           YHS+CI KW+Q KRVCPICGTEVSS K+SKNA
Sbjct: 181 YHSECISKWLQIKRVCPICGTEVSSSKSSKNA 211

BLAST of CmaCh15G006640 vs. ExPASy TrEMBL
Match: A0A6J1CJH0 (E3 ubiquitin ligase BIG BROTHER-related OS=Momordica charantia OX=3673 GN=LOC111011883 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.3e-79
Identity = 167/207 (80.68%), Postives = 175/207 (84.54%), Query Frame = 0

Query: 1   MGEQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDSDEDERSDSN 60
           M EQEDKQ  PQRISL YVEQVNSD IMALAMQQQEHE AYT L TIESDSDEDE  DSN
Sbjct: 1   MEEQEDKQP-PQRISLAYVEQVNSDFIMALAMQQQEHERAYTMLETIESDSDEDESFDSN 60

Query: 61  SNNGLDTNASLQSQ-------FLADNEETTDENEDMDED-DEEGFEDFDFDELTYEELIA 120
           SN GLDT   LQSQ       FLAD+EETTD  EDMDED DE+  +DFD DELTYEEL+A
Sbjct: 61  SNTGLDTYDPLQSQEFHSRWDFLADDEETTD--EDMDEDVDEDELDDFDLDELTYEELVA 120

Query: 121 LGEFIGEEKRGLPSNEIPSCLHSSKFRSIENTSGIDRCVICQVEYEDGEELAALPCEHPY 180
           LGEFIGEEKRGLP NEIPSCLHS KFR+IEN SG+DRCVICQVEYEDGEELAALPCEHPY
Sbjct: 121 LGEFIGEEKRGLPMNEIPSCLHSGKFRTIENKSGMDRCVICQVEYEDGEELAALPCEHPY 180

Query: 181 HSDCIVKWVQTKRVCPICGTEVSSPKA 200
           HS+CI KW+Q KRVCPICGTEVSSPKA
Sbjct: 181 HSECISKWLQIKRVCPICGTEVSSPKA 204

BLAST of CmaCh15G006640 vs. NCBI nr
Match: XP_022993933.1 (E3 ubiquitin ligase BIG BROTHER-related-like [Cucurbita maxima])

HSP 1 Score: 397.9 bits (1021), Expect = 5.3e-107
Identity = 203/203 (100.00%), Postives = 203/203 (100.00%), Query Frame = 0

Query: 1   MGEQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDSDEDERSDSN 60
           MGEQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDSDEDERSDSN
Sbjct: 1   MGEQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDSDEDERSDSN 60

Query: 61  SNNGLDTNASLQSQFLADNEETTDENEDMDEDDEEGFEDFDFDELTYEELIALGEFIGEE 120
           SNNGLDTNASLQSQFLADNEETTDENEDMDEDDEEGFEDFDFDELTYEELIALGEFIGEE
Sbjct: 61  SNNGLDTNASLQSQFLADNEETTDENEDMDEDDEEGFEDFDFDELTYEELIALGEFIGEE 120

Query: 121 KRGLPSNEIPSCLHSSKFRSIENTSGIDRCVICQVEYEDGEELAALPCEHPYHSDCIVKW 180
           KRGLPSNEIPSCLHSSKFRSIENTSGIDRCVICQVEYEDGEELAALPCEHPYHSDCIVKW
Sbjct: 121 KRGLPSNEIPSCLHSSKFRSIENTSGIDRCVICQVEYEDGEELAALPCEHPYHSDCIVKW 180

Query: 181 VQTKRVCPICGTEVSSPKASKNA 204
           VQTKRVCPICGTEVSSPKASKNA
Sbjct: 181 VQTKRVCPICGTEVSSPKASKNA 203

BLAST of CmaCh15G006640 vs. NCBI nr
Match: XP_023551485.1 (E3 ubiquitin ligase BIG BROTHER-related-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 361.3 bits (926), Expect = 5.4e-96
Identity = 191/206 (92.72%), Postives = 198/206 (96.12%), Query Frame = 0

Query: 1   MGEQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDS--DEDERSD 60
           MGEQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDS  DEDERSD
Sbjct: 1   MGEQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDSDEDEDERSD 60

Query: 61  SNSNNGLDTNASLQSQFLADNEETTDENEDMDEDDEEGFEDFDFDELTYEELIALGEFIG 120
           SNSNNGLDTNASLQSQFLAD+EE T+ENEDM EDD+EG+EDFDFDELTYEELIALGE IG
Sbjct: 61  SNSNNGLDTNASLQSQFLADDEE-TNENEDMAEDDDEGYEDFDFDELTYEELIALGELIG 120

Query: 121 EEKRGLPSNEIPSCLHSSKFRSIENTSGIDRCVICQVEYEDGEELAALPCEHPYHSDCIV 180
           EEKRGLP NEIPSCLHSSKF+SIENTSGIDRCVICQVEYEDGE+LAALPCEHPYHS+CIV
Sbjct: 121 EEKRGLPLNEIPSCLHSSKFQSIENTSGIDRCVICQVEYEDGEDLAALPCEHPYHSECIV 180

Query: 181 KWVQTKRVCPICGTEVSSP-KASKNA 204
           KWVQ KRVCPICGTEVSSP KASKNA
Sbjct: 181 KWVQIKRVCPICGTEVSSPHKASKNA 205

BLAST of CmaCh15G006640 vs. NCBI nr
Match: XP_038886321.1 (E3 ubiquitin ligase BIG BROTHER-related-like [Benincasa hispida] >XP_038886322.1 E3 ubiquitin ligase BIG BROTHER-related-like [Benincasa hispida] >XP_038886323.1 E3 ubiquitin ligase BIG BROTHER-related-like [Benincasa hispida])

HSP 1 Score: 327.0 bits (837), Expect = 1.1e-85
Identity = 171/210 (81.43%), Postives = 184/210 (87.62%), Query Frame = 0

Query: 1   MGEQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDSDEDERSDSN 60
           M EQEDKQQLPQRISL +VEQVNSDLIMALAMQQQEHEMAYT L TIESDSDEDE SDSN
Sbjct: 1   MEEQEDKQQLPQRISLAHVEQVNSDLIMALAMQQQEHEMAYTMLETIESDSDEDENSDSN 60

Query: 61  SNNGLDTNASLQSQ-------FLADNEETTDENEDMDEDDEEGFEDFDFDELTYEELIAL 120
           SN+GL+TNAS Q Q       FL D+EET DENEDM+ED++  FEDFD DELTYEELIAL
Sbjct: 61  SNHGLNTNASSQRQELLSRWAFLEDDEETADENEDMEEDEDGDFEDFDLDELTYEELIAL 120

Query: 121 GEFIGEEKRGLPSNEIPSCLHSSKFRSIENTSGIDRCVICQVEYEDGEELAALPCEHPYH 180
           GEFIG EKRGLP NEIPSCLH SKF++IEN SGIDRCVICQVEY++GEELAALPCEHPYH
Sbjct: 121 GEFIGTEKRGLPINEIPSCLHPSKFQTIENRSGIDRCVICQVEYDEGEELAALPCEHPYH 180

Query: 181 SDCIVKWVQTKRVCPICGTEVSSPKASKNA 204
           S+CI KW+Q KRVCPICGTEVSSPK SKNA
Sbjct: 181 SECIGKWLQIKRVCPICGTEVSSPKGSKNA 210

BLAST of CmaCh15G006640 vs. NCBI nr
Match: XP_004149804.1 (E3 ubiquitin ligase BIG BROTHER-related [Cucumis sativus] >KGN47704.1 hypothetical protein Csa_018241 [Cucumis sativus])

HSP 1 Score: 314.3 bits (804), Expect = 7.6e-82
Identity = 162/207 (78.26%), Postives = 180/207 (86.96%), Query Frame = 0

Query: 3   EQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDSDEDERSDSNSN 62
           +Q+ + QLPQRISL YVEQVN+DLIMALAMQQQEHE+AYTTL TI SDS+EDE SDSNSN
Sbjct: 10  QQQQQPQLPQRISLAYVEQVNTDLIMALAMQQQEHELAYTTLETIASDSEEDENSDSNSN 69

Query: 63  NGLDTNASLQSQ-------FLADNEETTDENEDMDEDDEEGFEDFDFDELTYEELIALGE 122
           NGLDTNA+ Q Q       FL D+EETTD N+DM+ED++  FE FD DELTYEELIALGE
Sbjct: 70  NGLDTNATSQRQELVSRWAFLEDDEETTDGNDDMEEDEDGDFEGFDLDELTYEELIALGE 129

Query: 123 FIGEEKRGLPSNEIPSCLHSSKFRSIENTSGIDRCVICQVEYEDGEELAALPCEHPYHSD 182
           FIGEEKRGLP NEIPSCLHSSKF++IEN SGIDRCVICQVEY+DGEELAALPCEHPYHS+
Sbjct: 130 FIGEEKRGLPINEIPSCLHSSKFQTIENRSGIDRCVICQVEYDDGEELAALPCEHPYHSE 189

Query: 183 CIVKWVQTKRVCPICGTEVSSPKASKN 203
           CI +W+Q KRVCPICGTEVSSP  SKN
Sbjct: 190 CIGEWLQIKRVCPICGTEVSSPNGSKN 216

BLAST of CmaCh15G006640 vs. NCBI nr
Match: XP_022954880.1 (E3 ubiquitin ligase BIG BROTHER-related [Cucurbita moschata])

HSP 1 Score: 310.8 bits (795), Expect = 8.4e-81
Identity = 170/212 (80.19%), Postives = 182/212 (85.85%), Query Frame = 0

Query: 1   MGEQEDKQQLPQRISLGYVEQVNSDLIMALAMQQQEHEMAYTTLGTIESDSDEDERSDSN 60
           M EQEDK QLPQRIS  YVEQVN+D IMALAMQQQEHE  YT L TIESDS+EDERSDSN
Sbjct: 1   MEEQEDK-QLPQRISRAYVEQVNTDFIMALAMQQQEHERNYTMLETIESDSEEDERSDSN 60

Query: 61  SNNGLD-TNASLQSQ-------FLADN-EETTDENEDMDEDDEEGFEDFDFDELTYEELI 120
           S+NGLD TN S QSQ       FLAD+ EETTD+NE MDED+EE  E+FD DEL+YEELI
Sbjct: 61  SSNGLDHTNDSFQSQELHSPWGFLADDEEETTDDNEYMDEDEEEDIEEFDLDELSYEELI 120

Query: 121 ALGEFIGEEKRGLPSNEIPSCLHSSKFRSIENTSGIDRCVICQVEYEDGEELAALPCEHP 180
           ALGEFIGEEKRGLP NEIPSCLHSSKF++IEN SGIDRCVICQVEYED EELAALPCEHP
Sbjct: 121 ALGEFIGEEKRGLPMNEIPSCLHSSKFQTIENKSGIDRCVICQVEYEDSEELAALPCEHP 180

Query: 181 YHSDCIVKWVQTKRVCPICGTEVSSPKASKNA 204
           YHS+CI KW+Q KRVCPICGTEVSSPK SKNA
Sbjct: 181 YHSECISKWLQIKRVCPICGTEVSSPKPSKNA 211

BLAST of CmaCh15G006640 vs. TAIR 10
Match: AT3G47180.1 (RING/U-box superfamily protein )

HSP 1 Score: 137.5 bits (345), Expect = 1.2e-32
Identity = 81/207 (39.13%), Postives = 127/207 (61.35%), Query Frame = 0

Query: 3   EQEDKQQLPQRI-SLGYVEQVNSDLIMALAMQQQE-----HEMAYTTLGTIESDSDEDER 62
           ++E+ +Q P ++  L   EQ NS++ +A +          H+ + + +  IESD + ++ 
Sbjct: 4   QEEEPKQPPNKLPDLALFEQANSEVALAASQANSHFAHAMHDSSPSMISMIESDEESEDE 63

Query: 63  SDSNSN-------NGLDTNASLQSQFLADNEETTDENEDMDEDDEEGFEDFDFDELTYEE 122
            + N N       NG   +    ++FL D E  ++  E+ D  +EE  ++ D D+L+YEE
Sbjct: 64  EEINENYYEYFDSNGFGVDEDEINEFLEDQESNSNLEEEDDFLEEE--DEIDPDQLSYEE 123

Query: 123 LIALGEFIGEEKRGLPSNEIPSCLHSSKFRSIENTSGIDRCVICQVEYEDGEELAAL-PC 182
           LIALG+FIG E RGL   EI +CL++S +    N + IDRCV+CQ+E+E+ E L  L PC
Sbjct: 124 LIALGDFIGVENRGLTPIEISTCLNASTYVFSHNKNEIDRCVVCQMEFEERESLVVLRPC 183

Query: 183 EHPYHSDCIVKWVQTKRVCPICGTEVS 196
           +HPYHS+CI KW++TK++CPIC +E S
Sbjct: 184 DHPYHSECITKWLETKKICPICCSEPS 208

BLAST of CmaCh15G006640 vs. TAIR 10
Match: AT3G19910.1 (RING/U-box superfamily protein )

HSP 1 Score: 99.8 bits (247), Expect = 2.8e-21
Identity = 58/170 (34.12%), Postives = 97/170 (57.06%), Query Frame = 0

Query: 48  ESDSDEDERSDSNSNNGLDTNASLQSQFLADNEE--------------TTDENEDMDEDD 107
           E D ++D  SD       D  A  ++   A+  +                ++ ED     
Sbjct: 172 EDDQEDDGNSDIEEVAYTDDEAYARALQEAEERDMAARLSALSGLANRVVEDLEDESHTS 231

Query: 108 EEGFEDFDFDELTYEELIALGEFIGEEKRGLPSNEIPSCLHSSKFRSIENTSGI-DRCVI 167
           ++ +++ D DEL+YEEL+ALG+ +G E RGL ++ I S L S +++  +N +G  + CVI
Sbjct: 232 QDAWDEMDPDELSYEELLALGDIVGTESRGLSADTIAS-LPSKRYKEGDNQNGTNESCVI 291

Query: 168 CQVEYEDGEELAALPCEHPYHSDCIVKWVQTKRVCPICGTEVSSPKASKN 203
           C+++YED E+L  LPC+H YHS+CI  W++  +VCP+C  EVS+  + ++
Sbjct: 292 CRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEVSTSTSGQS 340

BLAST of CmaCh15G006640 vs. TAIR 10
Match: AT3G63530.1 (RING/U-box superfamily protein )

HSP 1 Score: 92.8 bits (229), Expect = 3.4e-19
Identity = 50/121 (41.32%), Postives = 70/121 (57.85%), Query Frame = 0

Query: 77  ADNEETTDENEDMDEDDEEGFEDFDFDELTYEELIALGEFIGEEKRGLPSNEIPSCLHSS 136
           ++ EE      D D       +D D D +TYEEL+ LGE +G E RGL S E+   L + 
Sbjct: 122 SEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGL-SQELIETLPTK 181

Query: 137 KFR---SIENTSGIDRCVICQVEYEDGEELAALPCEHPYHSDCIVKWVQTKRVCPICGTE 195
           K++           +RCVICQ++Y+ GE    LPC+H YHS+CI KW+   +VCP+C +E
Sbjct: 182 KYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSE 241

BLAST of CmaCh15G006640 vs. TAIR 10
Match: AT3G63530.2 (RING/U-box superfamily protein )

HSP 1 Score: 92.8 bits (229), Expect = 3.4e-19
Identity = 50/121 (41.32%), Postives = 70/121 (57.85%), Query Frame = 0

Query: 77  ADNEETTDENEDMDEDDEEGFEDFDFDELTYEELIALGEFIGEEKRGLPSNEIPSCLHSS 136
           ++ EE      D D       +D D D +TYEEL+ LGE +G E RGL S E+   L + 
Sbjct: 122 SEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGL-SQELIETLPTK 181

Query: 137 KFR---SIENTSGIDRCVICQVEYEDGEELAALPCEHPYHSDCIVKWVQTKRVCPICGTE 195
           K++           +RCVICQ++Y+ GE    LPC+H YHS+CI KW+   +VCP+C +E
Sbjct: 182 KYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVCNSE 241

BLAST of CmaCh15G006640 vs. TAIR 10
Match: AT2G15530.1 (RING/U-box superfamily protein )

HSP 1 Score: 90.1 bits (222), Expect = 2.2e-18
Identity = 44/98 (44.90%), Postives = 56/98 (57.14%), Query Frame = 0

Query: 101 DFDELTYEELIALGEFIGEEKRGLPSNEIPSCLH-----SSKFRSIENTSGIDRCVICQV 160
           D D ++YEEL+ALGE IG+   GL    I   +      SS   S+E    I+ C ICQ 
Sbjct: 602 DVDNMSYEELLALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIEPCCICQE 661

Query: 161 EYEDGEELAALPCEHPYHSDCIVKWVQTKRVCPICGTE 194
           EY +G+ L  L C H +H DCI +WV  K +CPIC TE
Sbjct: 662 EYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LT173.9e-2034.12E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR P... [more]
Q8L6494.7e-1841.32E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE... [more]
Q9ZQF93.1e-1744.90E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV... [more]
Q5QLR52.2e-1540.71Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=399... [more]
Q7XTV72.9e-1542.39Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399... [more]
Match NameE-valueIdentityDescription
A0A6J1K3Q92.5e-107100.00E3 ubiquitin ligase BIG BROTHER-related-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A0A0KFS43.7e-8278.26RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G382920 P... [more]
A0A6J1GTM74.1e-8180.19E3 ubiquitin ligase BIG BROTHER-related OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1JR327.0e-8180.19E3 ubiquitin ligase BIG BROTHER-related-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1CJH01.3e-7980.68E3 ubiquitin ligase BIG BROTHER-related OS=Momordica charantia OX=3673 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_022993933.15.3e-107100.00E3 ubiquitin ligase BIG BROTHER-related-like [Cucurbita maxima][more]
XP_023551485.15.4e-9692.72E3 ubiquitin ligase BIG BROTHER-related-like [Cucurbita pepo subsp. pepo][more]
XP_038886321.11.1e-8581.43E3 ubiquitin ligase BIG BROTHER-related-like [Benincasa hispida] >XP_038886322.1... [more]
XP_004149804.17.6e-8278.26E3 ubiquitin ligase BIG BROTHER-related [Cucumis sativus] >KGN47704.1 hypothetic... [more]
XP_022954880.18.4e-8180.19E3 ubiquitin ligase BIG BROTHER-related [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G47180.11.2e-3239.13RING/U-box superfamily protein [more]
AT3G19910.12.8e-2134.12RING/U-box superfamily protein [more]
AT3G63530.13.4e-1941.32RING/U-box superfamily protein [more]
AT3G63530.23.4e-1941.32RING/U-box superfamily protein [more]
AT2G15530.12.2e-1844.90RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 150..190
e-value: 1.3E-5
score: 34.7
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 149..190
e-value: 2.0E-11
score: 44.1
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 150..190
score: 11.51558
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 127..200
e-value: 1.5E-17
score: 64.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 61..79
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 80..95
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 41..95
NoneNo IPR availablePANTHERPTHR46400:SF8E3 UBIQUITIN LIGASE BIG BROTHER-RELATED-LIKEcoord: 4..199
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 150..190
e-value: 9.34354E-16
score: 66.1713
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 149..197
IPR033276E3 ubiquitin-protein ligase BIG BROTHERPANTHERPTHR46400RING/U-BOX SUPERFAMILY PROTEINcoord: 4..199

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh15G006640.1CmaCh15G006640.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046621 negative regulation of organ growth
biological_process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0019774 proteasome core complex, beta-subunit complex
molecular_function GO:0004298 threonine-type endopeptidase activity
molecular_function GO:0031624 ubiquitin conjugating enzyme binding
molecular_function GO:0004842 ubiquitin-protein transferase activity