CmaCh15G006470 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh15G006470
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Calcium-binding EF-hand family protein
Location	Cma_Chr15: 3054726 .. 3055208 (-)
RNA-Seq Expression	CmaCh15G006470
Synteny	CmaCh15G006470

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCCTCCTATTAGCTCAAACGACTTGCTCAGGATCTTCAACAGGCTCGACAAGAACGGGGATGGCCTCATTTGCGTGCAGGAGCTCAAGTGGCTCCTAGACACCATTGGCATCCAATTGACCATGGAGGAGTTGGAGTCGTTTCTCGAGAAACCGAGCCTCGACTTCGATGAGTTCTTGATCTTTTATGAGTCGATGTCGAAGCAAAATAAAGGCGAGTGCAAGGGTGGGTTGGTTGATGAGCATGAAGATGAAGATGAAGATGTGGAGATTGTATATATGGCTTTTAAAGTGTTTGATGTGAATGGTGATGGCTTCATTTCTTGCGAGGAGCTCGAAAACGTGCTATCGAGGCTTGAGCTTTGGGATGCAAATAGAAGCGAGGTTGATTGTAGAAGCATGATCCGAGCTTATGACACCAATTTGGATGGGAAGCTGGATTTTGAAGAATTTATGAATATGATGTTGTTCACAAATTAA

mRNA sequence

ATGGCTCCTCCTATTAGCTCAAACGACTTGCTCAGGATCTTCAACAGGCTCGACAAGAACGGGGATGGCCTCATTTGCGTGCAGGAGCTCAAGTGGCTCCTAGACACCATTGGCATCCAATTGACCATGGAGGAGTTGGAGTCGTTTCTCGAGAAACCGAGCCTCGACTTCGATGAGTTCTTGATCTTTTATGAGTCGATGTCGAAGCAAAATAAAGGCGAGTGCAAGGGTGGGTTGGTTGATGAGCATGAAGATGAAGATGAAGATGTGGAGATTGTATATATGGCTTTTAAAGTGTTTGATGTGAATGGTGATGGCTTCATTTCTTGCGAGGAGCTCGAAAACGTGCTATCGAGGCTTGAGCTTTGGGATGCAAATAGAAGCGAGGTTGATTGTAGAAGCATGATCCGAGCTTATGACACCAATTTGGATGGGAAGCTGGATTTTGAAGAATTTATGAATATGATGTTGTTCACAAATTAA

Coding sequence (CDS)

ATGGCTCCTCCTATTAGCTCAAACGACTTGCTCAGGATCTTCAACAGGCTCGACAAGAACGGGGATGGCCTCATTTGCGTGCAGGAGCTCAAGTGGCTCCTAGACACCATTGGCATCCAATTGACCATGGAGGAGTTGGAGTCGTTTCTCGAGAAACCGAGCCTCGACTTCGATGAGTTCTTGATCTTTTATGAGTCGATGTCGAAGCAAAATAAAGGCGAGTGCAAGGGTGGGTTGGTTGATGAGCATGAAGATGAAGATGAAGATGTGGAGATTGTATATATGGCTTTTAAAGTGTTTGATGTGAATGGTGATGGCTTCATTTCTTGCGAGGAGCTCGAAAACGTGCTATCGAGGCTTGAGCTTTGGGATGCAAATAGAAGCGAGGTTGATTGTAGAAGCATGATCCGAGCTTATGACACCAATTTGGATGGGAAGCTGGATTTTGAAGAATTTATGAATATGATGTTGTTCACAAATTAA

Protein sequence

MAPPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLIFYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN

Homology

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BLAST of CmaCh15G006470 vs. ExPASy Swiss-Prot
Match: Q9LPK5 (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 PE=2 SV=2)

HSP 1 Score: 131.7 bits (330), Expect = 7.3e-30
Identity = 77/154 (50.00%), Postives = 97/154 (62.99%), Query Frame = 0


Query: 5   ISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIG-IQLTMEELESFLEKPSLDFDEFLIF 64
           I++NDL R+F  LDKN DGL+ + EL W+LD +G  + T +ELE  + K SLD DEFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 65  YESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLELW 124
           Y      +KG  K   +D   D D   E +  AF VFDVNGDG+IS EEL +VL RL  +
Sbjct: 66  YYDAVLDSKGSKKN--IDVVADND---EAIARAFNVFDVNGDGYISAEELRDVLERLG-F 125

Query: 125 DANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMML 158
           +      DC  MIR +D NLDG +DFEEF NM+L
Sbjct: 126 EEEAKAWDCGRMIRVHDKNLDGFVDFEEFKNMIL 153

[Close]

BLAST of CmaCh15G006470 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 5.2e-20
Identity = 64/165 (38.79%), Postives = 92/165 (55.76%), Query Frame = 0


Query: 2   APPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLE------KPSL 61
           +P +++  L RIF+  DKNGDG I V+EL   L  +G+   + +L+S +E         L
Sbjct: 22  SPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGL 81

Query: 62  DFDEFLIFYESMSKQNKGECKGGLVDEHEDED----EDVEIVYMAFKVFDVNGDGFISCE 121
           +FD+F   ++++     G   GG   E+ED+     E+   +  AFKVFD NGDGFIS  
Sbjct: 82  NFDDFSSLHKTLDDSFFGGACGG--GENEDDPSSAAENESDLAEAFKVFDENGDGFISAR 141

Query: 122 ELENVLSRLELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMM 157
           EL+ VL +L L +    E     MI + D N DG++DF EF NMM
Sbjct: 142 ELQTVLKKLGLPEGGEME-RVEKMIVSVDRNQDGRVDFFEFKNMM 183

[Close]

BLAST of CmaCh15G006470 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.2e-18
Identity = 59/154 (38.31%), Postives = 87/154 (56.49%), Query Frame = 0


Query: 9   DLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEK------PSLDFDEFLI 68
           +L R+F   DKNGDG I  +EL   L ++GI +  +EL   +EK        +D DEF  
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEF-- 64

Query: 69  FYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 128
                     GE    ++DE ++E+ED++    AF VFD NGDGFI+ +EL+ VLS L L
Sbjct: 65  ----------GELYKTIMDEEDEEEEDMK---EAFNVFDQNGDGFITVDELKAVLSSLGL 124

Query: 129 WDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMM 157
                 + DC+ MI+  D + DG+++++EF  MM
Sbjct: 125 KQGKTLD-DCKKMIKKVDVDGDGRVNYKEFRQMM 142

[Close]

BLAST of CmaCh15G006470 vs. ExPASy Swiss-Prot
Match: Q0JC44 (Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 GN=CML22 PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.1e-17
Identity = 57/154 (37.01%), Postives = 85/154 (55.19%), Query Frame = 0


Query: 9   DLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEK------PSLDFDEFLI 68
           +L R+F   D+NGDG I  +EL+  L  +GI +  +EL + + +        +D +EF  
Sbjct: 92  ELARVFELFDRNGDGRITREELEDSLGKLGIPVPADELAAVIARIDANGDGCVDVEEFGE 151

Query: 69  FYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 128
            Y S+         G   +E E+ED D+     AF+VFD NGDG+I+ +EL  VL+ L L
Sbjct: 152 LYRSIMAGGDDSKDGRAKEEEEEEDGDMR---EAFRVFDANGDGYITVDELGAVLASLGL 211

Query: 129 WDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMM 157
                +E +CR MI   D + DG++DF EF+ MM
Sbjct: 212 KQGRTAE-ECRRMIGQVDRDGDGRVDFHEFLQMM 241

[Close]

BLAST of CmaCh15G006470 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.1e-17
Identity = 58/164 (35.37%), Postives = 88/164 (53.66%), Query Frame = 0


Query: 2   APPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFL------EKPSL 61
           +P +++  L R+F+  DKN DG I V+EL   L  +G+     +L+S +      +K  L
Sbjct: 21  SPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGL 80

Query: 62  DFDEFLIFYESMSKQ---NKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEE 121
            FD+F   ++++ +     +G C  G  +   +E         AF VFD +GDGFIS  E
Sbjct: 81  RFDDFAALHKTLDESFFGGEGSCCDGSPESDLEE---------AFNVFDEDGDGFISAVE 140

Query: 122 LENVLSRLELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMM 157
           L+ VL +L L +A   E     MI + D+N DG++DF EF NMM
Sbjct: 141 LQKVLKKLGLPEAGEIE-QVEKMIVSVDSNHDGRVDFFEFKNMM 174

[Close]

BLAST of CmaCh15G006470 vs. ExPASy TrEMBL
Match: A0A6J1K1Y7 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111489388 PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 4.3e-86
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MAPPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           MAPPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF
Sbjct: 1   MAPPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LIFYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120
           LIFYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL
Sbjct: 61  LIFYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120

Query: 121 ELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 161
           ELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN
Sbjct: 121 ELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of CmaCh15G006470 vs. ExPASy TrEMBL
Match: A0A6J1FLU5 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111445399 PE=4 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 1.9e-81
Identity = 153/160 (95.62%), Postives = 156/160 (97.50%), Query Frame = 0


Query: 1   MAPPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           MA PISSNDLLRIFNRLDKN DGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF
Sbjct: 1   MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LIFYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120
           L FYES+SKQNKGECKGGLVDEH DE+EDVEIVYMAFKVFDVNGDGFISCEELENVLSRL
Sbjct: 61  LFFYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120

Query: 121 ELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 161
           ELWDAN+SEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN
Sbjct: 121 ELWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of CmaCh15G006470 vs. ExPASy TrEMBL
Match: A0A5D3B6U1 (Putative calcium-binding protein CML44 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold282G00080 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 6.5e-66
Identity = 132/161 (81.99%), Postives = 142/161 (88.20%), Query Frame = 0


Query: 4   PISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLIF 63
           PISS DL RIF +LDKN DGL+C+QELKWLLD IGIQLTMEELESFLEKPSLDFDEFL F
Sbjct: 3   PISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFF 62

Query: 64  YESMSKQNKGECKGGLV----DEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSR 123
           YES+SKQNKGECKGG+     D+HED   D EIVYMAFKVFD+NGDGFISC+ELENVL R
Sbjct: 63  YESISKQNKGECKGGVAGCVQDDHED---DTEIVYMAFKVFDMNGDGFISCDELENVLVR 122

Query: 124 LELWDANRSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 160
           LELWDA+RSEVD CRSMI+AYDTNLDGKLDFEEF NMML T
Sbjct: 123 LELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLT 160

[Close]

BLAST of CmaCh15G006470 vs. ExPASy TrEMBL
Match: A0A1S3B3T2 (probable calcium-binding protein CML44 OS=Cucumis melo OX=3656 GN=LOC103485689 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 6.5e-66
Identity = 132/161 (81.99%), Postives = 142/161 (88.20%), Query Frame = 0


Query: 4   PISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLIF 63
           PISS DL RIF +LDKN DGL+C+QELKWLLD IGIQLTMEELESFLEKPSLDFDEFL F
Sbjct: 3   PISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFLFF 62

Query: 64  YESMSKQNKGECKGGLV----DEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSR 123
           YES+SKQNKGECKGG+     D+HED   D EIVYMAFKVFD+NGDGFISC+ELENVL R
Sbjct: 63  YESISKQNKGECKGGVAGCVQDDHED---DTEIVYMAFKVFDMNGDGFISCDELENVLVR 122

Query: 124 LELWDANRSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 160
           LELWDA+RSEVD CRSMI+AYDTNLDGKLDFEEF NMML T
Sbjct: 123 LELWDASRSEVDYCRSMIQAYDTNLDGKLDFEEFKNMMLLT 160

[Close]

BLAST of CmaCh15G006470 vs. ExPASy TrEMBL
Match: A0A0A0KFQ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376250 PE=4 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 7.2e-65
Identity = 128/163 (78.53%), Postives = 143/163 (87.73%), Query Frame = 0


Query: 3   PPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLI 62
           PPISS DL RIF +LDKN DGLIC+QEL WLLD+IGIQLTMEELESFLE+PSLDFDEFL 
Sbjct: 2   PPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFLF 61

Query: 63  FYESMSKQNKGECKGGLVDEHEDED-----EDVEIVYMAFKVFDVNGDGFISCEELENVL 122
           FYES+SKQNKGECKGG+    +D D     +D+EIVY+AFKVFD+NGDGFISC+ELENVL
Sbjct: 62  FYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENVL 121

Query: 123 SRLELWDANRSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 160
            +LELWDA+RS+VD CRSMIRAYDTNLDGKLDFEEF NMML T
Sbjct: 122 VKLELWDASRSDVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLT 164

[Close]

BLAST of CmaCh15G006470 vs. NCBI nr
Match: XP_022993348.1 (probable calcium-binding protein CML44 [Cucurbita maxima])

HSP 1 Score: 327.0 bits (837), Expect = 9.0e-86
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MAPPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           MAPPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF
Sbjct: 1   MAPPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LIFYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120
           LIFYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL
Sbjct: 61  LIFYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120

Query: 121 ELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 161
           ELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN
Sbjct: 121 ELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of CmaCh15G006470 vs. NCBI nr
Match: XP_023551499.1 (probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 312.8 bits (800), Expect = 1.8e-81
Identity = 154/160 (96.25%), Postives = 156/160 (97.50%), Query Frame = 0


Query: 1   MAPPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           MA PISSNDLLRIFNRLDKN DGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF
Sbjct: 1   MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LIFYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120
           L FYES+SKQNKGECKGGLVDEH DE+EDVEIVYMAFKVFDVNGDGFISCEELENVLSRL
Sbjct: 61  LFFYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120

Query: 121 ELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 161
           ELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN
Sbjct: 121 ELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of CmaCh15G006470 vs. NCBI nr
Match: XP_022939513.1 (probable calcium-binding protein CML44 [Cucurbita moschata] >KAG7016457.1 putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 311.6 bits (797), Expect = 3.9e-81
Identity = 153/160 (95.62%), Postives = 156/160 (97.50%), Query Frame = 0


Query: 1   MAPPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           MA PISSNDLLRIFNRLDKN DGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF
Sbjct: 1   MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LIFYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120
           L FYES+SKQNKGECKGGLVDEH DE+EDVEIVYMAFKVFDVNGDGFISCEELENVLSRL
Sbjct: 61  LFFYESISKQNKGECKGGLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120

Query: 121 ELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 161
           ELWDAN+SEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN
Sbjct: 121 ELWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of CmaCh15G006470 vs. NCBI nr
Match: KAG6578937.1 (putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 307.0 bits (785), Expect = 9.6e-80
Identity = 151/160 (94.38%), Postives = 155/160 (96.88%), Query Frame = 0


Query: 1   MAPPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           MA PISSNDLLRIFNRLDKN DGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF
Sbjct: 1   MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LIFYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120
           L FYES+SKQNKGECKGGLV EH DE+EDVEIVYMAFKVFDVNGDGFISCEELENVLSRL
Sbjct: 61  LFFYESISKQNKGECKGGLVYEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRL 120

Query: 121 ELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 161
           ELWDAN++EVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN
Sbjct: 121 ELWDANKNEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]

BLAST of CmaCh15G006470 vs. NCBI nr
Match: XP_038885742.1 (probable calcium-binding protein CML44 [Benincasa hispida])

HSP 1 Score: 264.2 bits (674), Expect = 7.1e-67
Identity = 129/156 (82.69%), Postives = 143/156 (91.67%), Query Frame = 0


Query: 5   ISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLIFY 64
           IS NDL RIFN+LDKNGDGLIC+QELKWL D IGIQLTMEELESFLEKPSLDF+EFL FY
Sbjct: 14  ISFNDLHRIFNKLDKNGDGLICLQELKWLFDRIGIQLTMEELESFLEKPSLDFNEFLFFY 73

Query: 65  ESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLELWD 124
           ES+SKQNKGECKGG+    +D +E++EIVYMAFKVFD+NGDGFISC+ELENVLSRLELWD
Sbjct: 74  ESISKQNKGECKGGVGCLQDDHEEEMEIVYMAFKVFDMNGDGFISCDELENVLSRLELWD 133

Query: 125 ANRSEVD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 160
           ANR+++D CRSMIRAYDTNLDGKLDFEEF NMML T
Sbjct: 134 ANRNDIDYCRSMIRAYDTNLDGKLDFEEFKNMMLLT 169

[Close]

BLAST of CmaCh15G006470 vs. TAIR 10
Match: AT1G21550.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 131.7 bits (330), Expect = 5.2e-31
Identity = 77/154 (50.00%), Postives = 97/154 (62.99%), Query Frame = 0


Query: 5   ISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIG-IQLTMEELESFLEKPSLDFDEFLIF 64
           I++NDL R+F  LDKN DGL+ + EL W+LD +G  + T +ELE  + K SLD DEFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 65  YESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLELW 124
           Y      +KG  K   +D   D D   E +  AF VFDVNGDG+IS EEL +VL RL  +
Sbjct: 66  YYDAVLDSKGSKKN--IDVVADND---EAIARAFNVFDVNGDGYISAEELRDVLERLG-F 125

Query: 125 DANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMML 158
           +      DC  MIR +D NLDG +DFEEF NM+L
Sbjct: 126 EEEAKAWDCGRMIRVHDKNLDGFVDFEEFKNMIL 153

[Close]

BLAST of CmaCh15G006470 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 99.0 bits (245), Expect = 3.7e-21
Identity = 64/165 (38.79%), Postives = 92/165 (55.76%), Query Frame = 0


Query: 2   APPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLE------KPSL 61
           +P +++  L RIF+  DKNGDG I V+EL   L  +G+   + +L+S +E         L
Sbjct: 22  SPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGL 81

Query: 62  DFDEFLIFYESMSKQNKGECKGGLVDEHEDED----EDVEIVYMAFKVFDVNGDGFISCE 121
           +FD+F   ++++     G   GG   E+ED+     E+   +  AFKVFD NGDGFIS  
Sbjct: 82  NFDDFSSLHKTLDDSFFGGACGG--GENEDDPSSAAENESDLAEAFKVFDENGDGFISAR 141

Query: 122 ELENVLSRLELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMM 157
           EL+ VL +L L +    E     MI + D N DG++DF EF NMM
Sbjct: 142 ELQTVLKKLGLPEGGEME-RVEKMIVSVDRNQDGRVDFFEFKNMM 183

[Close]

BLAST of CmaCh15G006470 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 93.6 bits (231), Expect = 1.6e-19
Identity = 59/154 (38.31%), Postives = 87/154 (56.49%), Query Frame = 0


Query: 9   DLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEK------PSLDFDEFLI 68
           +L R+F   DKNGDG I  +EL   L ++GI +  +EL   +EK        +D DEF  
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEF-- 64

Query: 69  FYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 128
                     GE    ++DE ++E+ED++    AF VFD NGDGFI+ +EL+ VLS L L
Sbjct: 65  ----------GELYKTIMDEEDEEEEDMK---EAFNVFDQNGDGFITVDELKAVLSSLGL 124

Query: 129 WDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMM 157
                 + DC+ MI+  D + DG+++++EF  MM
Sbjct: 125 KQGKTLD-DCKKMIKKVDVDGDGRVNYKEFRQMM 142

[Close]

BLAST of CmaCh15G006470 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 91.3 bits (225), Expect = 7.7e-19
Identity = 58/164 (35.37%), Postives = 88/164 (53.66%), Query Frame = 0


Query: 2   APPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFL------EKPSL 61
           +P +++  L R+F+  DKN DG I V+EL   L  +G+     +L+S +      +K  L
Sbjct: 21  SPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGL 80

Query: 62  DFDEFLIFYESMSKQ---NKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEE 121
            FD+F   ++++ +     +G C  G  +   +E         AF VFD +GDGFIS  E
Sbjct: 81  RFDDFAALHKTLDESFFGGEGSCCDGSPESDLEE---------AFNVFDEDGDGFISAVE 140

Query: 122 LENVLSRLELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMM 157
           L+ VL +L L +A   E     MI + D+N DG++DF EF NMM
Sbjct: 141 LQKVLKKLGLPEAGEIE-QVEKMIVSVDSNHDGRVDFFEFKNMM 174

[Close]

BLAST of CmaCh15G006470 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 89.7 bits (221), Expect = 2.2e-18
Identity = 58/154 (37.66%), Postives = 83/154 (53.90%), Query Frame = 0


Query: 9   DLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEK------PSLDFDEFLI 68
           +L R+F   DKNGDG I   ELK    ++GI +   E+   + K       ++D DEF  
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGS 64

Query: 69  FYESMSKQNKGECKGGLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLEL 128
            Y+ M            V+E E+E++  E    AF+VFD NGDGFI+ EEL +VL+ + L
Sbjct: 65  LYQEM------------VEEKEEEEDMRE----AFRVFDQNGDGFITDEELRSVLASMGL 124

Query: 129 WDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMM 157
               R+  DC+ MI   D + DG ++F+EF  MM
Sbjct: 125 -KQGRTLEDCKKMISKVDVDGDGMVNFKEFKQMM 141

The following BLAST results are available for this feature:

BLAST of CmaCh15G006470 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LPK5	7.3e-30	50.00	Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 ...	[more]
Q9SVG9	5.2e-20	38.79	Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1	[more]
Q9LNE7	2.2e-18	38.31	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q0JC44	1.1e-17	37.01	Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9FI19	1.1e-17	35.37	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ...	[more]

BLAST of CmaCh15G006470 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K1Y7	4.3e-86	100.00	probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114893...	[more]
A0A6J1FLU5	1.9e-81	95.63	probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A5D3B6U1	6.5e-66	81.99	Putative calcium-binding protein CML44 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3B3T2	6.5e-66	81.99	probable calcium-binding protein CML44 OS=Cucumis melo OX=3656 GN=LOC103485689 P...	[more]
A0A0A0KFQ6	7.2e-65	78.53	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376250 PE=4 SV=1	[more]

BLAST of CmaCh15G006470 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022993348.1	9.0e-86	100.00	probable calcium-binding protein CML44 [Cucurbita maxima]	[more]
XP_023551499.1	1.8e-81	96.25	probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo]	[more]
XP_022939513.1	3.9e-81	95.63	probable calcium-binding protein CML44 [Cucurbita moschata] >KAG7016457.1 putati...	[more]
KAG6578937.1	9.6e-80	94.38	putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_038885742.1	7.1e-67	82.69	probable calcium-binding protein CML44 [Benincasa hispida]	[more]

BLAST of CmaCh15G006470 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G21550.1	5.2e-31	50.00	Calcium-binding EF-hand family protein	[more]
AT4G20780.1	3.7e-21	38.79	calmodulin like 42	[more]
AT1G05990.1	1.6e-19	38.31	EF hand calcium-binding protein family	[more]
AT5G44460.1	7.7e-19	35.37	calmodulin like 43	[more]
AT4G12860.1	2.2e-18	37.66	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 9..37 e-value: 0.0023 score: 27.2 coord: 131..159 e-value: 0.17 score: 21.0 coord: 92..120 e-value: 0.0022 score: 27.2
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 94..156 e-value: 1.0E-11 score: 45.1
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 13..31 e-value: 2.9E-5 score: 23.3
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 88..123 score: 13.286007
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 127..160 score: 13.230217
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 5..40 score: 12.365459
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 9..61 e-value: 9.47825E-8 score: 44.8461
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 96..156 e-value: 3.84996E-13 score: 58.7133
None	No IPR available	GENE3D	1.10.238.10		coord: 65..156 e-value: 1.1E-19 score: 72.6
None	No IPR available	GENE3D	1.10.238.10		coord: 5..64 e-value: 1.4E-10 score: 43.2
None	No IPR available	PANTHER	PTHR10891:SF788	CALCIUM-BINDING PROTEIN CML44-RELATED	coord: 9..160
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 9..160
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 101..113
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 18..30
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 8..157

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh15G006470.1	CmaCh15G006470.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding