CmaCh15G003580 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh15G003580
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionglycosylphosphatidylinositol anchor attachment 1 protein-like
LocationCma_Chr15: 1644068 .. 1647962 (-)
RNA-Seq ExpressionCmaCh15G003580
SyntenyCmaCh15G003580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTAACTGAGCTCACATTCACTTCTTCTGCCCTTTTCTTCTTCTTCTTCTTGGTTTCCATTTTTGCAACAGATTCAGAGCAATTGGGCGACTGCAAAGAGCACCTCTTCCGATGGCTGAAACCGAGAAGCCAAAAACGAAGGCGCGACCGATTGTGCGCCTGGGAATTTTCCTAATTTCTCACAGTATTTTCTTCAGGTGCGCTGTTGTGATTTTACATGGATTTGATTTTCAGTTCGTATCAGAATCTAGTGATTGATTAATGTCCTTGTGATTTTCTTTTATTTTTTGGCTGTTTTCTAGCGTGATTTGCTTCTCTGCAGGGGTTTTAGCTCTTCTGTTACTACCAATGTTTGCTATGAACACCTACATTTCCGAGAACGCTCTCATGCCAGGTCCCTTTGATATTCATCATTTTTTGTATTTTCGTGATTGAACGCCAGTTCAGTCGAATGATTTAAGATATATTACGAATTAGGATTCATTTGAAGCTGCGAAGTATCTCAAATTATAGGATCATCAAATTTATGCCTTCTTGTTCTTTTGCACTGATCTATTCATTTTACCTTTATTTGGATAGAACAGGTCTCTTAATTGTGCCACTATATTTTCCCACAGGTTCTGCAAATACCATGCTCTCGGGTCAGGAAGTCTCAGAGGCGAACGACTTGGTGAAGGATCTGAAGGGTTTGAATTCGAAGTCTGGCTCAACGATCTTGTAAGTACTTATGCACTAGTTTTATCCCTTTTACTTCTCCTTTAATACCTTAATGGAAGCTTTTTTATGCTTAAGATTTTGAGAATCACCACTTCTTAGTGTAAATTTTTTCTACCTTAAGATTATGATAAACAAATTTGATTTACAATACATTTTTCAGTGGAAGCCAGCAGATTTTAGCACAATATATATCAAACTTGGGTGCAGAAGTTAATTACCACAGGTTCCAACCGCAATCTAGTCAGTTCCATCCATTGCACTTTTTTTCTAGCCCTGATTCTGGGATCGTTCAGGACAATGTTAGTTGCATGACACAGGGTATTAACACTGTTGGGATAATAAGAGCACCACAAGCAGATGGAAAGGAAGCCATCGTTTTGGTGACACCTTACAATCCTGTTAAGACTAGTCTTCATGAGACTTTGTCTCTTGGTATTGCTTATTCAATATTTTCTTTGCTTACACAAGTCAGTTGGTTGGCCAAGGACATTGTATGGCTTGCTGCGGATTCACGATATGGGGAATATGCAGCAGTTTCAGCTTGGCTTAGAGATTATCACACTCCTTCTTTTGGTCGATCCAACATGATTGACACATATACTTGTGCTGAAACAAATGTCCTTTATGAATTTGAAGCAAACCATGTAACAGAGAAAGAGAGTCTAGATGATTTTAAACGTGCTGGAACGATGGCTGCAGCACTTGTCATTAAGGTTTCAAATAGAAGTGAACACTTTGAGGATAGTCTTAGTGTCTATGCTGAAGCGTCTAATGGTCAGATGCCGAACCTCGACCTCATCAATATTGTGAATTATCTAGCAGAACACAGACAAGGTTTTCGGATTAAGATCGAGAAATTTTGGCCTTTACTTGATTGCAAATGGCTCAAGTTTTTGGGCGAATTTTTTGAGTCAATAGGAAAGTTTATCCGAAGCTTGAACCCTGAATGGAAGTTTGGCATGCTAGCTTCTGACTATGTCGATGGCACCGCTACACTTGCAAGTTCATTGTACTACCAGGTGGTTTGCACTTCTGTTCATATTCTTGGAGATTGTTGTTTATGACTTCATTTAATGAAATAAGGAAAGATTTTTCATTAATGTGAAAAATGGAGATTATTTCACTTAAATTATCTATGTTCTTTTTGCCATTAGGCTGTGGGTATTCCTACTGGTTCTCATGGAGCTTTTCGAGATTACCAAATTGATGCAATTACTGTGGAGATGTCCCCCAAATTTTCTTCTGGTGTCAAGGTCAGGCGTGATGAATTCATACTGCGAGGTGGCCGGTCAGTGCTCATTTTTGCCTCAAGGATTTTATAATGTGCTCTCCTCCCACCCCTAACTTTTCAGGGCTCACTACATGAATAAATGTTTAAAACCAATGGTTTCCTTAATGGGGTTCGGTCTACATTAAACAATGTTTTCTACTCTTATTTTAATTAGACATCATTTTCCATTTTTATCTTAAGTGTTTCACTGTCGATCTTCAATGTTTAAATTTTAATTTGTAACTAGTTTTAATGGGGGCATGAGTAAATACTTTTTCTTCCCTAAAATAAATTTTGATGGACTGATAGTTTCTCTTTTTAAATGACTAGAATTTACGTGTTAAATTCATTCAAGTGAATTTATTGCCTAAAGTCATTTTGATGTATGGAAATGAGTTTAGATTTTAACGCCTCGAATGATCAAAATATTCATAATTGGATTTGAAATTAGAAGTTGACAACTTACCGCCCAGCTTGCTGGCAATTCTGCATGCTAGCAACGAAACTCTCCTGATCCTTTTGATTTGGCTAAGGATTCTGTATTTGGTTGTGCAGGCTAATTGAAGGAGTTGTACGGTCAGTGAATAACCTCTTGGAGAAGTTTCATCAATCATTCTTTCTATACCTTATGGTATCTACCAGCAAATTTGTATCAGTTGGTGTATATATGATTGCCTTTGCTCTCCTAGTTGCTCCACTACCAGCAGTTGCGGCCTCTCTCTATTCTTATGCTAATAACTCGAATCTAACCTTGGAAAAAATCGAACCTGCAGCTCCAGCAAATTCTGATGATGAGCTTATTGTCTCTTTAAGATCATGGAAATGGCTTAATGCTGCAAAAAGAGTTTTTGTTGTTCATCTATGGGGTGCTGTTGTTTCATTGCTTCCATACTTCATCTCCCAAATACCTGGTTACAGTCCCACAACAAACTCTATTATTTGGGCGTTACTTTCACTGCTCACGCTACTAATTTTGTCGGTGATATTGGGCTCTCCACTTAGTTCAACTAAATCTTATGAACAGCGGATTCAAGAGTGGGCGTTCTTGAAAGCAATGACCACCTCAGCGGCCTTTATCGGTTTGTGCCTCATGTCAGTAATTAACTTTGCTACTGCAGAACTTGGAGCCTTCTTGGTGGTGTCCATGTGCTTGCTGGCGCATCCCCTGAAGCTTGATTTAGTGGCTGGGAACTTCAAAGCTCTCTCAAGGGCAGCCAGTAACCTCGTTTTGGGATTCATAGCTTTTCCGCCTGTTACTTTCTTCTTGTTTAAAGCTGCTTTGCAAGGTTTTGACAATTTACATCTCGGGGACTTCTGGAACTGGATGGAGACCCTCTGGGCATGGAACAGTGCTACTTTCCTCTACTTAGGTATGGTTCACCTTCCTTGCTGGGTATTATGTACACAAATTTTACTTCATCCATGTTGAGCTACCTTGTTTAGTTTTTTCCATGACATTTGTTTAGACTTTCACTTAGTCCTGAGAAATTGGTTTCGATGGTATTTGAAGTAGTTACACGTTCATCCCTTGCATGGTAGCTTCTGTAAGCAGGCCTTAAAGCCTACTACTTTGTGTCACAGTTGTTTATACTTGTAGGACTGATACAGTAAGCTTTTACGCTCTTGGAAATTGTTTTTGGCTATTTCTCTTTTCGAACTAATTGGACAATCACCTTCTCTATATCCAATGATCAGTGTCTCTTGAGTTTCTCTCTCCAATTACTGCATTTAAAGATTATAGCACATAGGCAAATGTAGAATTTAGCAACAAATTAGCTGCTGAGGTTTCTTTTAATCCTTGTGAGCCCGTGACTAGGTCCATCCATAGTGTTTCTATGAGATCTTAGATTAGTAGGAGAATGAAACGAAACATTTCTTAGGGTGTGAAAATCTTTTCTTAGAAGACGC

mRNA sequence

GTTAACTGAGCTCACATTCACTTCTTCTGCCCTTTTCTTCTTCTTCTTCTTGGTTTCCATTTTTGCAACAGATTCAGAGCAATTGGGCGACTGCAAAGAGCACCTCTTCCGATGGCTGAAACCGAGAAGCCAAAAACGAAGGCGCGACCGATTGTGCGCCTGGGAATTTTCCTAATTTCTCACAGTATTTTCTTCAGCGTGATTTGCTTCTCTGCAGGGGTTTTAGCTCTTCTGTTACTACCAATGTTTGCTATGAACACCTACATTTCCGAGAACGCTCTCATGCCAGGTTCTGCAAATACCATGCTCTCGGGTCAGGAAGTCTCAGAGGCGAACGACTTGGTGAAGGATCTGAAGGGTTTGAATTCGAAGTCTGGCTCAACGATCTTTGGAAGCCAGCAGATTTTAGCACAATATATATCAAACTTGGGTGCAGAAGTTAATTACCACAGGTTCCAACCGCAATCTAGTCAGTTCCATCCATTGCACTTTTTTTCTAGCCCTGATTCTGGGATCGTTCAGGACAATGTTAGTTGCATGACACAGGGTATTAACACTGTTGGGATAATAAGAGCACCACAAGCAGATGGAAAGGAAGCCATCGTTTTGGTGACACCTTACAATCCTGTTAAGACTAGTCTTCATGAGACTTTGTCTCTTGGTATTGCTTATTCAATATTTTCTTTGCTTACACAAGTCAGTTGGTTGGCCAAGGACATTGTATGGCTTGCTGCGGATTCACGATATGGGGAATATGCAGCAGTTTCAGCTTGGCTTAGAGATTATCACACTCCTTCTTTTGGTCGATCCAACATGATTGACACATATACTTGTGCTGAAACAAATGTCCTTTATGAATTTGAAGCAAACCATGTAACAGAGAAAGAGAGTCTAGATGATTTTAAACGTGCTGGAACGATGGCTGCAGCACTTGTCATTAAGGTTTCAAATAGAAGTGAACACTTTGAGGATAGTCTTAGTGTCTATGCTGAAGCGTCTAATGGTCAGATGCCGAACCTCGACCTCATCAATATTGTGAATTATCTAGCAGAACACAGACAAGGTTTTCGGATTAAGATCGAGAAATTTTGGCCTTTACTTGATTGCAAATGGCTCAAGTTTTTGGGCGAATTTTTTGAGTCAATAGGAAAGTTTATCCGAAGCTTGAACCCTGAATGGAAGTTTGGCATGCTAGCTTCTGACTATGTCGATGGCACCGCTACACTTGCAAGTTCATTGTACTACCAGGCTGTGGGTATTCCTACTGGTTCTCATGGAGCTTTTCGAGATTACCAAATTGATGCAATTACTGTGGAGATGTCCCCCAAATTTTCTTCTGGTGTCAAGGTCAGGCGTGATGAATTCATACTGCGAGGTGGCCGGCTAATTGAAGGAGTTGTACGGTCAGTGAATAACCTCTTGGAGAAGTTTCATCAATCATTCTTTCTATACCTTATGGTATCTACCAGCAAATTTGTATCAGTTGGTGTATATATGATTGCCTTTGCTCTCCTAGTTGCTCCACTACCAGCAGTTGCGGCCTCTCTCTATTCTTATGCTAATAACTCGAATCTAACCTTGGAAAAAATCGAACCTGCAGCTCCAGCAAATTCTGATGATGAGCTTATTGTCTCTTTAAGATCATGGAAATGGCTTAATGCTGCAAAAAGAGTTTTTGTTGTTCATCTATGGGGTGCTGTTGTTTCATTGCTTCCATACTTCATCTCCCAAATACCTGGTTACAGTCCCACAACAAACTCTATTATTTGGGCGTTACTTTCACTGCTCACGCTACTAATTTTGTCGGTGATATTGGGCTCTCCACTTAGTTCAACTAAATCTTATGAACAGCGGATTCAAGAGTGGGCGTTCTTGAAAGCAATGACCACCTCAGCGGCCTTTATCGGTTTGTGCCTCATGTCAGTAATTAACTTTGCTACTGCAGAACTTGGAGCCTTCTTGGTGGTGTCCATGTGCTTGCTGGCGCATCCCCTGAAGCTTGATTTAGTGGCTGGGAACTTCAAAGCTCTCTCAAGGGCAGCCAGTAACCTCGTTTTGGGATTCATAGCTTTTCCGCCTGTTACTTTCTTCTTGTTTAAAGCTGCTTTGCAAGGTTTTGACAATTTACATCTCGGGGACTTCTGGAACTGGATGGAGACCCTCTGGGCATGGAACAGTGCTACTTTCCTCTACTTAGGTATGGTTCACCTTCCTTGCTGGGTATTATGTACACAAATTTTACTTCATCCATGTTGAGCTACCTTGTTTAGTTTTTTCCATGACATTTGTTTAGACTTTCACTTAGTCCTGAGAAATTGGTTTCGATGGTATTTGAAGTAGTTACACGTTCATCCCTTGCATGGTAGCTTCTGTAAGCAGGCCTTAAAGCCTACTACTTTGTGTCACAGTTGTTTATACTTGTAGGACTGATACAGTAAGCTTTTACGCTCTTGGAAATTGTTTTTGGCTATTTCTCTTTTCGAACTAATTGGACAATCACCTTCTCTATATCCAATGATCAGTGTCTCTTGAGTTTCTCTCTCCAATTACTGCATTTAAAGATTATAGCACATAGGCAAATGTAGAATTTAGCAACAAATTAGCTGCTGAGGTTTCTTTTAATCCTTGTGAGCCCGTGACTAGGTCCATCCATAGTGTTTCTATGAGATCTTAGATTAGTAGGAGAATGAAACGAAACATTTCTTAGGGTGTGAAAATCTTTTCTTAGAAGACGC

Coding sequence (CDS)

ATGGCTGAAACCGAGAAGCCAAAAACGAAGGCGCGACCGATTGTGCGCCTGGGAATTTTCCTAATTTCTCACAGTATTTTCTTCAGCGTGATTTGCTTCTCTGCAGGGGTTTTAGCTCTTCTGTTACTACCAATGTTTGCTATGAACACCTACATTTCCGAGAACGCTCTCATGCCAGGTTCTGCAAATACCATGCTCTCGGGTCAGGAAGTCTCAGAGGCGAACGACTTGGTGAAGGATCTGAAGGGTTTGAATTCGAAGTCTGGCTCAACGATCTTTGGAAGCCAGCAGATTTTAGCACAATATATATCAAACTTGGGTGCAGAAGTTAATTACCACAGGTTCCAACCGCAATCTAGTCAGTTCCATCCATTGCACTTTTTTTCTAGCCCTGATTCTGGGATCGTTCAGGACAATGTTAGTTGCATGACACAGGGTATTAACACTGTTGGGATAATAAGAGCACCACAAGCAGATGGAAAGGAAGCCATCGTTTTGGTGACACCTTACAATCCTGTTAAGACTAGTCTTCATGAGACTTTGTCTCTTGGTATTGCTTATTCAATATTTTCTTTGCTTACACAAGTCAGTTGGTTGGCCAAGGACATTGTATGGCTTGCTGCGGATTCACGATATGGGGAATATGCAGCAGTTTCAGCTTGGCTTAGAGATTATCACACTCCTTCTTTTGGTCGATCCAACATGATTGACACATATACTTGTGCTGAAACAAATGTCCTTTATGAATTTGAAGCAAACCATGTAACAGAGAAAGAGAGTCTAGATGATTTTAAACGTGCTGGAACGATGGCTGCAGCACTTGTCATTAAGGTTTCAAATAGAAGTGAACACTTTGAGGATAGTCTTAGTGTCTATGCTGAAGCGTCTAATGGTCAGATGCCGAACCTCGACCTCATCAATATTGTGAATTATCTAGCAGAACACAGACAAGGTTTTCGGATTAAGATCGAGAAATTTTGGCCTTTACTTGATTGCAAATGGCTCAAGTTTTTGGGCGAATTTTTTGAGTCAATAGGAAAGTTTATCCGAAGCTTGAACCCTGAATGGAAGTTTGGCATGCTAGCTTCTGACTATGTCGATGGCACCGCTACACTTGCAAGTTCATTGTACTACCAGGCTGTGGGTATTCCTACTGGTTCTCATGGAGCTTTTCGAGATTACCAAATTGATGCAATTACTGTGGAGATGTCCCCCAAATTTTCTTCTGGTGTCAAGGTCAGGCGTGATGAATTCATACTGCGAGGTGGCCGGCTAATTGAAGGAGTTGTACGGTCAGTGAATAACCTCTTGGAGAAGTTTCATCAATCATTCTTTCTATACCTTATGGTATCTACCAGCAAATTTGTATCAGTTGGTGTATATATGATTGCCTTTGCTCTCCTAGTTGCTCCACTACCAGCAGTTGCGGCCTCTCTCTATTCTTATGCTAATAACTCGAATCTAACCTTGGAAAAAATCGAACCTGCAGCTCCAGCAAATTCTGATGATGAGCTTATTGTCTCTTTAAGATCATGGAAATGGCTTAATGCTGCAAAAAGAGTTTTTGTTGTTCATCTATGGGGTGCTGTTGTTTCATTGCTTCCATACTTCATCTCCCAAATACCTGGTTACAGTCCCACAACAAACTCTATTATTTGGGCGTTACTTTCACTGCTCACGCTACTAATTTTGTCGGTGATATTGGGCTCTCCACTTAGTTCAACTAAATCTTATGAACAGCGGATTCAAGAGTGGGCGTTCTTGAAAGCAATGACCACCTCAGCGGCCTTTATCGGTTTGTGCCTCATGTCAGTAATTAACTTTGCTACTGCAGAACTTGGAGCCTTCTTGGTGGTGTCCATGTGCTTGCTGGCGCATCCCCTGAAGCTTGATTTAGTGGCTGGGAACTTCAAAGCTCTCTCAAGGGCAGCCAGTAACCTCGTTTTGGGATTCATAGCTTTTCCGCCTGTTACTTTCTTCTTGTTTAAAGCTGCTTTGCAAGGTTTTGACAATTTACATCTCGGGGACTTCTGGAACTGGATGGAGACCCTCTGGGCATGGAACAGTGCTACTTTCCTCTACTTAGGTATGGTTCACCTTCCTTGCTGGGTATTATGTACACAAATTTTACTTCATCCATGTTGA

Protein sequence

MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPGSANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSSQFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYTCAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQIPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC
Homology
BLAST of CmaCh15G003580 vs. ExPASy Swiss-Prot
Match: O43292 (Glycosylphosphatidylinositol anchor attachment 1 protein OS=Homo sapiens OX=9606 GN=GPAA1 PE=1 SV=3)

HSP 1 Score: 115.2 bits (287), Expect = 3.1e-24
Identity = 178/715 (24.90%), Postives = 288/715 (40.28%), Query Frame = 0

Query: 10  KARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPGSANTMLSGQ 69
           + R + RL + L +     S +   A  LAL+  P+    TY+SENA+         +G 
Sbjct: 9   RRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPL-TQRTYMSENAMGSTMVEEQFAGG 68

Query: 70  EVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSSQFHPLHFFS 129
           +   A    +D      KSG+        L + + ++G EV    F  +           
Sbjct: 69  D--RARAFARDFAAHRKKSGAL---PVAWLERTMRSVGLEVYTQSFSRK---------LP 128

Query: 130 SPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIAYSI 189
            PD    + +   M  G N  GI+RAP+A   E++VL  P     T+   + ++G+  ++
Sbjct: 129 FPD----ETHERYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTN---SQAVGLLLAL 188

Query: 190 FSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYTCAETNVLYE 249
            +      + AKDIV+L  +    +     AWL  YH                       
Sbjct: 189 AAHFRGQIYWAKDIVFLVTEH---DLLGTEAWLEAYHDV--------------------- 248

Query: 250 FEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLINIV 309
               +VT  +S     RAG + AA+ +++S+       SL V  E  NGQ+PNLDL+N+ 
Sbjct: 249 ----NVTGMQSSPLQGRAGAIQAAVALELSS---DVVTSLDVAVEGLNGQLPNLDLLNLF 308

Query: 310 NYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPE-WKFGMLASDYVDG 369
               + + G    ++                     GK    L PE W         + G
Sbjct: 309 QTFCQ-KGGLLCTLQ---------------------GK----LQPEDW---TSLDGPLQG 368

Query: 370 TATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEF-ILRGGRLIE 429
             TL   +  QA G P GSHG F  Y+++A+T+     F      R+ ++ ++  G+ +E
Sbjct: 369 LQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRGINSF------RQYKYDLVAVGKALE 428

Query: 430 GVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNSN 489
           G+ R +N+LLE+ HQSFFLYL+   S+FVS+G+YM A   L+  L   A  L+   + + 
Sbjct: 429 GMFRKLNHLLERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAG 488

Query: 490 LTLEKIEP-AAPANSDDELIVSLRSWKWLNAAKRV---FVVHLWGAVVSLLPYFISQIPG 549
           + LE  EP  AP  S     V L   + +  A  V    +    G  + +LP     +  
Sbjct: 489 MGLE--EPGGAPGPS-----VPLPPSQGVGLASLVAPLLISQAMGLALYVLPVLGQHVAT 548

Query: 550 YSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGLCLM 609
                      +L+LL +    + L        S +   + W  LK +      + L  +
Sbjct: 549 QHFPVAEAEAVVLTLLAIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIYLALQLGCI 608

Query: 610 SVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFFLFK 669
           ++ NF+   L A  +V    LA P     +      L+  A+ L LG       + FL++
Sbjct: 609 ALTNFSLGFLLATTMVPTAALAKPHGPRTLYAALLVLTSPAATL-LG-------SLFLWR 618

Query: 670 AALQGFDNLHLGDFWNWMETLWA--------WNSATFLYLGMVHLPCWVLCTQIL 711
              +    L L + W       A        + +  F  L +   PCW+L   +L
Sbjct: 669 ELQEA--PLSLAEGWQLFLAALAQGVLEHHTYGALLFPLLSLGLYPCWLLFWNVL 618

BLAST of CmaCh15G003580 vs. ExPASy Swiss-Prot
Match: Q9WTK3 (Glycosylphosphatidylinositol anchor attachment 1 protein OS=Mus musculus OX=10090 GN=Gpaa1 PE=1 SV=3)

HSP 1 Score: 110.5 bits (275), Expect = 7.7e-23
Identity = 170/723 (23.51%), Postives = 288/723 (39.83%), Query Frame = 0

Query: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLL--PMFAMNTYISENALM 60
           +++  + +  AR ++RL   L        V+ + AG+   L L  P     TY+SENA+ 
Sbjct: 4   LSDPVRRRALARIVLRLNTPL-------CVLSYVAGIAWFLALAFPPLTQRTYMSENAM- 63

Query: 61  PGSANTMLSGQEV--SEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQ 120
               +TM+  Q V    A    +D      K G+        L + + ++G EV    F 
Sbjct: 64  ---GSTMVEEQFVGGDRARSFARDFAAHRKKPGAL---PVAWLERSMRSVGLEVYTQSFS 123

Query: 121 PQSSQFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTS 180
            +            PD    + +   M  G N  GI+RAP++   E++VL  P  P  T+
Sbjct: 124 RK---------LPFPD----ETHERYMVSGTNVYGILRAPRSASTESLVLTVPCGPDATN 183

Query: 181 LHETLSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMI 240
              + ++G+  ++ +      + AKDI++L  D    +     AWL  YH          
Sbjct: 184 ---SQAVGLLLALAAHFRGQIYWAKDIIFLVTDH---DLLGTEAWLEAYHD--------- 243

Query: 241 DTYTCAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEAS 300
                            +VT  +S     RAG + AA+ +++S+       SL V  E  
Sbjct: 244 ----------------INVTGIQSSPLQGRAGAIQAAVALELSS---DVVTSLDVTVEGL 303

Query: 301 NGQMPNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEW 360
           NGQ+PNLDL+N+     + + G    ++    L    W    G                 
Sbjct: 304 NGQLPNLDLLNLFQTFCQ-KGGLLCTLQ--GKLQPQDWTSLEGP---------------- 363

Query: 361 KFGMLASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRD 420
                    + G  TL   +  QA G P G HG F  Y ++A+T+     F      R+ 
Sbjct: 364 ---------LQGLQTLLLMVLRQASGRPHGPHGLFLRYGVEALTLRGINSF------RQY 423

Query: 421 EFILRG-GRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAV 480
           ++ L   G+ +EG+ R +N+LLE+ HQSFF YL+ + S+FVS+G+YM A   L+  L   
Sbjct: 424 KYDLATVGKALEGMFRKLNHLLERLHQSFFFYLLPALSRFVSIGLYMPATGFLLLVLGLK 483

Query: 481 AASLYSYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLP 540
           A  L+   + + +  E+   A  A S    ++  +     +    + +    G  +  LP
Sbjct: 484 ALELWMQLHQAGVNPEE---AGKAPSPGTPLLPTQGVGLASLTAPLLISQAMGLALYFLP 543

Query: 541 YFISQIPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSA 600
                +             +L+LL + +  + L        + +   + W  LK +    
Sbjct: 544 VLGQHLATQHFPVAEAEAVVLTLLAIYVAGLALPHNTHRVVNSQVPDRGWMALKLVALIY 603

Query: 601 AFIGLCLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFP 660
             + L  ++++NF+   L A  +V    LA P     +   + AL    S  V  F    
Sbjct: 604 LALQLGCIALLNFSLGFLLAATMVPAAALAKPHGPRTL---YAALLVVTSPAVTLF---- 618

Query: 661 PVTFFLFKAALQGFDNLHLGDFWNWMETL--------WAWNSATFLYLGMVHLPCWVLCT 711
             + FL++  L+    L L + W    T         + + +  F  L +   PCW+L  
Sbjct: 664 -GSLFLWRELLE--VPLSLAEGWQLFLTALAQGVLEHYTYGALLFPILALGLYPCWLLFW 618

BLAST of CmaCh15G003580 vs. ExPASy Swiss-Prot
Match: Q9US48 (GPI transamidase component gaa1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gaa1 PE=3 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 1.5e-18
Identity = 109/462 (23.59%), Postives = 181/462 (39.18%), Query Frame = 0

Query: 15  VRLGIFLISHSIFFSVICFSAGVLALLLLPMFAM--NTYISENALMPGSANTMLSGQEVS 74
           +R+  FL  H  F  +     G+  + +LP   +    ++SE+AL+PG  NT    +   
Sbjct: 9   IRVFPFLQRHLFFLQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYFENRY-- 68

Query: 75  EANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSSQFHPLHFFSSPD 134
                            S    S    A   S+L A V  +       Q        +  
Sbjct: 69  -----------------SKTVSSSLTAANTWSHLDASVGTNTMYDDLEQIFTAMGLPTQK 128

Query: 135 SGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIAYSIFSL 194
                +       G N +  +RAP+ D  E+++L  P+       +E   + +A S+   
Sbjct: 129 QNYSINIPGSEFNGSNFITTLRAPRGDATESLLLCVPWKDHIGQYNEA-GVALAISLLKY 188

Query: 195 LTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYTCAETNVLYEFEA 254
               S  +KDI+ +  D                  P +G S+ + +Y             
Sbjct: 189 FQGWSLWSKDIILVIFDD-----------------PVYGPSSFLTSY------------F 248

Query: 255 NHVTEKESLDDFK-RAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLINIVNY 314
           +  T   S    K R+G++ A L +++   +E+  D L V  +A+NGQ+PNLDL N ++ 
Sbjct: 249 DQTTPYISYTPLKIRSGSIQAGLSLELVT-TENNSDVLEVLYQATNGQLPNLDLFNTISR 308

Query: 315 LAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGMLASDYVDGTAT 374
           +      + ++++ +             +F  + G                S Y     +
Sbjct: 309 IFMQHFNYPLRLQGY-------------DFHANSG----------------SSYTSRLKS 368

Query: 375 LASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEF---ILRGGRLIEG 434
           L   +  QAV   T +H  F  Y+ID +T+ M  K         D F   + R G+ IE 
Sbjct: 369 LWMGMLTQAVSNVTSAHALFPQYRIDMLTLRMKVK---------DPFSFDMFRFGQAIES 382

Query: 435 VVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVA 471
             RS+NNLLE  HQSFF Y ++    F+S+G YM +  +L A
Sbjct: 429 TFRSLNNLLEHLHQSFFFYFILDHLHFISIGNYMPSILILAA 382

BLAST of CmaCh15G003580 vs. ExPASy Swiss-Prot
Match: P39012 (GPI transamidase component GAA1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GAA1 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 3.8e-14
Identity = 169/746 (22.65%), Postives = 286/746 (38.34%), Query Frame = 0

Query: 12  RPIVRLGIF--LISHSIFFSVICFSAGVLALLLLPMFAM--NTYISENALMPGSANTMLS 71
           R IV +G+   +I+     S++C   G +++ +LPM      TYISENALMP  A +   
Sbjct: 9   RRIVDMGLVPRIIALLPVISMLCALFGFISIAILPMDGQYRRTYISENALMPSQAYSYFR 68

Query: 72  GQE---VSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSSQFHP 131
             E   +      +K++  + S   + + GS      ++   G +   +    ++ Q+  
Sbjct: 69  ESEWNILRGYRSQIKEMVNMTSMERNNLMGS------WLQEFGTKTAIY----ENEQY-- 128

Query: 132 LHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTP-YNPVKTSLHETLSL 191
                                G    G++ AP+ DG EA+VL  P +N          +L
Sbjct: 129 ---------------------GETLYGVMHAPRGDGTEAMVLAVPWFNSDDEFNIGGAAL 188

Query: 192 GIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYTCAE 251
           G++ + F     V W    IV  + + R    AA+ +W+  YHT                
Sbjct: 189 GVSLARFFSRWPV-WSKNIIVVFSENPR----AALRSWVEAYHT---------------- 248

Query: 252 TNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNL 311
                           SLD     G++ AA+V+  S+  + FE  + +  +  NG++PNL
Sbjct: 249 ----------------SLD--LTGGSIEAAVVLDYSSTEDFFE-YVEISYDGLNGELPNL 308

Query: 312 DLINIVNYLAEHRQGFRIKI----------EKFWPLLDCKWLKFLGEFFESIGKFIRSLN 371
           DL+NI   + EH +G ++ +            FW  L    L                  
Sbjct: 309 DLVNIAISITEH-EGMKVSLHGLPSDQLTNNNFWSRLKILCLGI---------------- 368

Query: 372 PEWKFGMLASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKV 431
            +W                        V  P G+  AF  ++I ++T++     +SG   
Sbjct: 369 RDWAL--------------------SGVKKPHGNE-AFSGWRIQSVTLKAHG--NSG--- 428

Query: 432 RRDEFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYM-IAFALLVAPL 491
                I   GR+ E + RS+NNLLEKFHQSFF YL+++  +FVS+  Y+  A AL +A  
Sbjct: 429 ---HDITTFGRIPEAMFRSINNLLEKFHQSFFFYLLLAPRQFVSISSYLPSAVALSIA-- 488

Query: 492 PAVAASLYSYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVS 551
               +SL ++ NN+   +            +  +V+L  W                 V  
Sbjct: 489 -FAISSLNAFINNAYANISLF--------SEYNLVALLVW----------------FVSL 548

Query: 552 LLPYFISQIPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMT 611
           ++ + +SQ     P++  +   +   +    L +IL   +  ++    R++  AFL    
Sbjct: 549 VISFVVSQAFLLIPSSGLL---MTISMASCFLPLILSRKIHISEPLSYRLKNVAFLYFSL 594

Query: 612 TSAAFIGLCLMSVINFATAELGAFLVVSMCLL----------------AHPLK------- 671
            S + +      +INFA A L   L   M  +                ++P+K       
Sbjct: 609 VSTSLL------MINFAMALLIGTLAFPMTFVKTIVESSSEHEVTTQSSNPIKTEPKDEI 594

Query: 672 ------LDLVAGNFKALSRAASNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMET 706
                 +D      +   +   NLVL  +  P ++  LF   L   D  H  D  N + +
Sbjct: 669 ELVENHMDTTPATPQQQKQKLKNLVLLILTNPFISITLF--GLFFDDEFHGFDIINKLVS 594

BLAST of CmaCh15G003580 vs. ExPASy TrEMBL
Match: A0A6J1K1W5 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489371 PE=4 SV=1)

HSP 1 Score: 1401.7 bits (3627), Expect = 0.0e+00
Identity = 714/714 (100.00%), Postives = 714/714 (100.00%), Query Frame = 0

Query: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120
           SANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS
Sbjct: 61  SANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180
           QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET
Sbjct: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240
           LSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT
Sbjct: 181 LSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240

Query: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360
           PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM
Sbjct: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360

Query: 361 LASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420
           LASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQ 540
           SYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQ
Sbjct: 481 SYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQ 540

Query: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600
           IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660
           CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 715
           LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of CmaCh15G003580 vs. ExPASy TrEMBL
Match: A0A6J1FHQ4 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445544 PE=4 SV=1)

HSP 1 Score: 1372.8 bits (3552), Expect = 0.0e+00
Identity = 698/714 (97.76%), Postives = 707/714 (99.02%), Query Frame = 0

Query: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKPK+KARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFA+NTYISENALMPG
Sbjct: 1   MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAINTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120
           SANTMLSGQEVSEANDLVKD+KGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS
Sbjct: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180
           +FHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET
Sbjct: 121 RFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240
           LSLGIAYSIFSLLTQV+WLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRS+MIDTYT
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT 240

Query: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360
           PNLDLINIVNYLAEHRQGF+IKIEKFWPLLDCKWLKFLGE FE+IGKFIRSLNPEWKFGM
Sbjct: 301 PNLDLINIVNYLAEHRQGFQIKIEKFWPLLDCKWLKFLGEIFETIGKFIRSLNPEWKFGM 360

Query: 361 LASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420
           LASDYVDGTATLASSLYYQAVGIPTG HGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQ 540
           SYANNSNLTLEKIEPAAPAN DDELIVSLRSW+WLNAAKRVFVVHLWGAVVSLLPYFI  
Sbjct: 481 SYANNSNLTLEKIEPAAPANPDDELIVSLRSWRWLNAAKRVFVVHLWGAVVSLLPYFICP 540

Query: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600
           IP YSPTTNSIIWALLSLLTLLILSVILGSPLSSTKS EQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPDYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660
           CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 715
           LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of CmaCh15G003580 vs. ExPASy TrEMBL
Match: A0A6J1JZW5 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489371 PE=4 SV=1)

HSP 1 Score: 1321.6 bits (3419), Expect = 0.0e+00
Identity = 670/670 (100.00%), Postives = 670/670 (100.00%), Query Frame = 0

Query: 45  MFAMNTYISENALMPGSANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYIS 104
           MFAMNTYISENALMPGSANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYIS
Sbjct: 1   MFAMNTYISENALMPGSANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYIS 60

Query: 105 NLGAEVNYHRFQPQSSQFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAI 164
           NLGAEVNYHRFQPQSSQFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAI
Sbjct: 61  NLGAEVNYHRFQPQSSQFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAI 120

Query: 165 VLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRD 224
           VLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRD
Sbjct: 121 VLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRD 180

Query: 225 YHTPSFGRSNMIDTYTCAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEH 284
           YHTPSFGRSNMIDTYTCAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEH
Sbjct: 181 YHTPSFGRSNMIDTYTCAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEH 240

Query: 285 FEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFES 344
           FEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFES
Sbjct: 241 FEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFES 300

Query: 345 IGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMS 404
           IGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMS
Sbjct: 301 IGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMS 360

Query: 405 PKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIA 464
           PKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIA
Sbjct: 361 PKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIA 420

Query: 465 FALLVAPLPAVAASLYSYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVV 524
           FALLVAPLPAVAASLYSYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVV
Sbjct: 421 FALLVAPLPAVAASLYSYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVV 480

Query: 525 HLWGAVVSLLPYFISQIPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQE 584
           HLWGAVVSLLPYFISQIPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQE
Sbjct: 481 HLWGAVVSLLPYFISQIPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQE 540

Query: 585 WAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAA 644
           WAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAA
Sbjct: 541 WAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAA 600

Query: 645 SNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWV 704
           SNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWV
Sbjct: 601 SNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWV 660

Query: 705 LCTQILLHPC 715
           LCTQILLHPC
Sbjct: 661 LCTQILLHPC 670

BLAST of CmaCh15G003580 vs. ExPASy TrEMBL
Match: A0A6J1FMH6 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445544 PE=4 SV=1)

HSP 1 Score: 1294.3 bits (3348), Expect = 0.0e+00
Identity = 655/670 (97.76%), Postives = 663/670 (98.96%), Query Frame = 0

Query: 45  MFAMNTYISENALMPGSANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYIS 104
           MFA+NTYISENALMPGSANTMLSGQEVSEANDLVKD+KGLNSKSGSTIFGSQQILAQYIS
Sbjct: 1   MFAINTYISENALMPGSANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYIS 60

Query: 105 NLGAEVNYHRFQPQSSQFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAI 164
           NLGAEVNYHRFQPQSS+FHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAI
Sbjct: 61  NLGAEVNYHRFQPQSSRFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAI 120

Query: 165 VLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRD 224
           VLVTPYNPVKTSLHETLSLGIAYSIFSLLTQV+WLAKDIVWLAADSRYGEYAAVSAWLRD
Sbjct: 121 VLVTPYNPVKTSLHETLSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRD 180

Query: 225 YHTPSFGRSNMIDTYTCAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEH 284
           YHTPSFGRS+MIDTYTCAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEH
Sbjct: 181 YHTPSFGRSSMIDTYTCAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEH 240

Query: 285 FEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFES 344
           FEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGF+IKIEKFWPLLDCKWLKFLGE FE+
Sbjct: 241 FEDSLSVYAEASNGQMPNLDLINIVNYLAEHRQGFQIKIEKFWPLLDCKWLKFLGEIFET 300

Query: 345 IGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMS 404
           IGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPTG HGAFRDYQIDAITVEMS
Sbjct: 301 IGKFIRSLNPEWKFGMLASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMS 360

Query: 405 PKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIA 464
           PKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIA
Sbjct: 361 PKFSSGVKVRRDEFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIA 420

Query: 465 FALLVAPLPAVAASLYSYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVV 524
           FALLVAPLPAVAASLYSYANNSNLTLEKIEPAAPAN DDELIVSLRSW+WLNAAKRVFVV
Sbjct: 421 FALLVAPLPAVAASLYSYANNSNLTLEKIEPAAPANPDDELIVSLRSWRWLNAAKRVFVV 480

Query: 525 HLWGAVVSLLPYFISQIPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQE 584
           HLWGAVVSLLPYFI  IP YSPTTNSIIWALLSLLTLLILSVILGSPLSSTKS EQRIQE
Sbjct: 481 HLWGAVVSLLPYFICPIPDYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQE 540

Query: 585 WAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAA 644
           WAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAA
Sbjct: 541 WAFLKAMTTSAAFIGLCLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAA 600

Query: 645 SNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWV 704
           SNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWV
Sbjct: 601 SNLVLGFIAFPPVTFFLFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWV 660

Query: 705 LCTQILLHPC 715
           LCTQILLHPC
Sbjct: 661 LCTQILLHPC 670

BLAST of CmaCh15G003580 vs. ExPASy TrEMBL
Match: A0A0A0KP05 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523060 PE=4 SV=1)

HSP 1 Score: 1293.9 bits (3347), Expect = 0.0e+00
Identity = 656/714 (91.88%), Postives = 682/714 (95.52%), Query Frame = 0

Query: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEK K KARPIVRLGIFLISHSIFFSV+CFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120
           SAN+MLSG+EVSEAN+LVKDLKGLNSK GS+IFGSQQILAQYIS LGA+VNYHRF PQSS
Sbjct: 61  SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS 120

Query: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180
           QFHPLHFFSSPDSGI++DNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPV+TSLH+T
Sbjct: 121 QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180

Query: 181 LSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240
           LSLGIAYSIFSLLTQV+WLAKDIVWL ADSRYGEYAAVSAWLRDYHTP FG+S++IDT  
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA 240

Query: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           C+ETNVL EFEAN VTEK  LDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360
           PNLDLINIVNYLA HRQGFRIKIEKFWPLL+CKWLK LGE FESIGK IRSLN EWKFGM
Sbjct: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM 360

Query: 361 LASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420
            ASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSG+KVRRD+FIL
Sbjct: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVST KFVSVGVYMIAFALLVAPLPAVAA+LY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480

Query: 481 SYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQ 540
           SYANN NLT EK+EP A AN DDELIVSLRSWKWLNAAKRVFVVHLWGA+VSLLPYFI Q
Sbjct: 481 SYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQ 540

Query: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600
           IPGYSPT NSIIW LLSLL+LL+LSVILGSP SSTKSYEQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660
           CLMSVINF+TAELGAFLVVSMCLLAHPLKLDL AGNFKALSRAA NLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660

Query: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 715
           LFK ALQGFDNLH+GDFWNWMETLWAWNSATFLYLGMVHLPCW+LCTQILLHPC
Sbjct: 661 LFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWLLCTQILLHPC 714

BLAST of CmaCh15G003580 vs. NCBI nr
Match: XP_022993328.1 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1401.7 bits (3627), Expect = 0.0e+00
Identity = 714/714 (100.00%), Postives = 714/714 (100.00%), Query Frame = 0

Query: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120
           SANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS
Sbjct: 61  SANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180
           QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET
Sbjct: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240
           LSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT
Sbjct: 181 LSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240

Query: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360
           PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM
Sbjct: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360

Query: 361 LASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420
           LASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQ 540
           SYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQ
Sbjct: 481 SYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQ 540

Query: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600
           IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660
           CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 715
           LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of CmaCh15G003580 vs. NCBI nr
Match: XP_023550991.1 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1380.5 bits (3572), Expect = 0.0e+00
Identity = 703/714 (98.46%), Postives = 708/714 (99.16%), Query Frame = 0

Query: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKPK+KARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120
           SANTMLSGQEVSEANDLVKD+KGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS
Sbjct: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180
           QFHPLHFFSSPDSGIVQDNVSCM+QGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET
Sbjct: 121 QFHPLHFFSSPDSGIVQDNVSCMSQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240
           LSLGIAYSIFSLLTQV+WLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRS+MIDTYT
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT 240

Query: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360
           PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM
Sbjct: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360

Query: 361 LASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420
           LASDYVDGTATLASSLYYQAVGIPTG HGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQ 540
           SYANN NLTLEKIEPAAPAN DD LIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFI Q
Sbjct: 481 SYANNLNLTLEKIEPAAPANPDDVLIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQ 540

Query: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600
           IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKS EQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660
           CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 715
           LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of CmaCh15G003580 vs. NCBI nr
Match: KAG7016207.1 (Glycosylphosphatidylinositol anchor attachment 1 protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1375.9 bits (3560), Expect = 0.0e+00
Identity = 699/714 (97.90%), Postives = 706/714 (98.88%), Query Frame = 0

Query: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAG+LALLLLPMFA+NTYISENALMPG
Sbjct: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGILALLLLPMFALNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120
           SANTMLSGQEVSEANDLVKD+KGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS
Sbjct: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180
           QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET
Sbjct: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240
           LSLGIAYSIFSLLTQV+WLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240

Query: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           CAETNVLYEFEANHVTEKESLDDFKRAGTMAAAL+IKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALIIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360
           PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGE FE+IGKFIRSLNPEWKFGM
Sbjct: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEIFETIGKFIRSLNPEWKFGM 360

Query: 361 LASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420
           LASDYVDGTATLASSLYYQAVGIPTG HGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQ 540
           SYANN NLTLEKIEPAAPAN DDELIVSLRSW+WLNAAKRVFVVHLWGAVVSLLPYFI Q
Sbjct: 481 SYANNLNLTLEKIEPAAPANPDDELIVSLRSWRWLNAAKRVFVVHLWGAVVSLLPYFICQ 540

Query: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600
           IP YSPTTNSIIWALLSLLTLLILSVILGSPLSSTKS EQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPDYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660
           CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVA NFKALSRAASNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAWNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 715
           LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of CmaCh15G003580 vs. NCBI nr
Match: XP_022939757.1 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1372.8 bits (3552), Expect = 0.0e+00
Identity = 698/714 (97.76%), Postives = 707/714 (99.02%), Query Frame = 0

Query: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKPK+KARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFA+NTYISENALMPG
Sbjct: 1   MAETEKPKSKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAINTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120
           SANTMLSGQEVSEANDLVKD+KGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS
Sbjct: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180
           +FHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET
Sbjct: 121 RFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240
           LSLGIAYSIFSLLTQV+WLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRS+MIDTYT
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSSMIDTYT 240

Query: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360
           PNLDLINIVNYLAEHRQGF+IKIEKFWPLLDCKWLKFLGE FE+IGKFIRSLNPEWKFGM
Sbjct: 301 PNLDLINIVNYLAEHRQGFQIKIEKFWPLLDCKWLKFLGEIFETIGKFIRSLNPEWKFGM 360

Query: 361 LASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420
           LASDYVDGTATLASSLYYQAVGIPTG HGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQ 540
           SYANNSNLTLEKIEPAAPAN DDELIVSLRSW+WLNAAKRVFVVHLWGAVVSLLPYFI  
Sbjct: 481 SYANNSNLTLEKIEPAAPANPDDELIVSLRSWRWLNAAKRVFVVHLWGAVVSLLPYFICP 540

Query: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600
           IP YSPTTNSIIWALLSLLTLLILSVILGSPLSSTKS EQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPDYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660
           CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 715
           LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHPC 714

BLAST of CmaCh15G003580 vs. NCBI nr
Match: KAG6578667.1 (Glycosylphosphatidylinositol anchor attachment 1 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1327.4 bits (3434), Expect = 0.0e+00
Identity = 679/695 (97.70%), Postives = 685/695 (98.56%), Query Frame = 0

Query: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60
           MA TEKPKTKARPIVRLGIFLISHSIFFSVICFSAG+LALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAGTEKPKTKARPIVRLGIFLISHSIFFSVICFSAGILALLLLPMFAMNTYISENALMPG 60

Query: 61  SANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120
           SANTMLSGQEVSEANDLVKD+KGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS
Sbjct: 61  SANTMLSGQEVSEANDLVKDMKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180
           QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET
Sbjct: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240
           LSLGIAYSIFSLLTQV+WLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240

Query: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           CAETNVLYEFEANHVTEKESLDDFKRAGTMAAAL+IKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALIIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360
           PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGE FE+IGKFIRSLNPEWKFGM
Sbjct: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEIFETIGKFIRSLNPEWKFGM 360

Query: 361 LASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420
           LASDYVDGTATLASSLYYQAVGIPTG HGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGPHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQ 540
           SYANN NLTLEKIEPAAPAN DDELIVSLRSW+WLNAAKRVFVVHLWGAVVSLLP FI Q
Sbjct: 481 SYANNLNLTLEKIEPAAPANPDDELIVSLRSWRWLNAAKRVFVVHLWGAVVSLLPNFICQ 540

Query: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600
           IP YSPTTNSIIWALLSLLTLLILSVILGSPLSSTKS EQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPDYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSCEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660
           CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVA NFKALSRAASNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAWNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYL 696
           LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYL
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYL 695

BLAST of CmaCh15G003580 vs. TAIR 10
Match: AT5G19130.1 (GPI transamidase component family protein / Gaa1-like family protein )

HSP 1 Score: 771.5 bits (1991), Expect = 5.7e-223
Identity = 411/710 (57.89%), Postives = 515/710 (72.54%), Query Frame = 0

Query: 7   PKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPGSANTML 66
           PK K RPIVRLGIFLI+HS  FSV+  +AGVLALLLLP+ A NTYISENALMPGSA +ML
Sbjct: 19  PKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALMPGSARSML 78

Query: 67  SGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSSQFHPLH 126
           S ++VS+ + LVKD+K             Q+++ +Y+S++GAEV+Y +F P+ +QFHPLH
Sbjct: 79  SNRDVSDGSKLVKDIKNFRLNHEGQGVEVQKLIGKYMSDMGAEVSYQKFHPEGNQFHPLH 138

Query: 127 FFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIA 186
           FFS PDS  + +NVSC + G+N  GIIRAP+ DGKE+IVLVTPY+ +    +E LSLGI 
Sbjct: 139 FFSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGIV 198

Query: 187 YSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYTCAETNV 246
            S+FSLL++V+WL+KDI+WL ADSRYG+Y  V+AWL +YH+PSF  S+++    C E N 
Sbjct: 199 SSLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSDLL---KCDEQN- 258

Query: 247 LYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLI 306
                        + D+F+RAGTMAAALV+KV  RSE FED+LS+YAEASNGQMPNLDLI
Sbjct: 259 -------------TADNFRRAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLI 318

Query: 307 NIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGMLASDYV 366
           N+VNYLA HRQGF +K+EK   LL   WLK  GE FE++GK    LNP+W FG+ A+DY+
Sbjct: 319 NVVNYLAVHRQGFYVKVEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYL 378

Query: 367 DGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLI 426
           +G+ATLASSLY QA+GIPTG HGAFRDYQ+DAIT+++SP+F    K R+ +F LRG RL+
Sbjct: 379 EGSATLASSLYSQALGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGARLL 438

Query: 427 EGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNS 486
           EG +RSVNNLLEKFHQSFFLY++ S SKF+SVGVYMIAFALLVAPLP VAASLY     S
Sbjct: 439 EGTIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKS 498

Query: 487 NLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQIPG-YS 546
            LT          NS      + +SWKWL+AAK+VF +HL G +V+LLPYFI Q+PG +S
Sbjct: 499 -LT----------NSTHNPTENFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQVPGQHS 558

Query: 547 PTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGLCLMSV 606
           PT  SI+W   S   LLI  V +  P  S  S       WA LK++T SAAFIGLCLMS+
Sbjct: 559 PTNRSIMWGTTSSSLLLITFVTM--PGCSPFSSRLHGTNWAILKSVTISAAFIGLCLMSI 618

Query: 607 INFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFFLFKAA 666
           INFATAE+GA L+V  CL+A P+K  L +   K+L  A  ++VL  I FP + F + K  
Sbjct: 619 INFATAEIGALLLVPTCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAISKGL 678

Query: 667 L-QGFDNLHL-GDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHP 714
           L +G   L L G+FW W+E+LWAW SAT+LY+GMVHLPCW+LC  IL HP
Sbjct: 679 LGEGLVGLSLGGEFWTWLESLWAWKSATYLYIGMVHLPCWLLCLCILFHP 698

BLAST of CmaCh15G003580 vs. TAIR 10
Match: AT5G19130.2 (GPI transamidase component family protein / Gaa1-like family protein )

HSP 1 Score: 770.8 bits (1989), Expect = 9.8e-223
Identity = 411/710 (57.89%), Postives = 516/710 (72.68%), Query Frame = 0

Query: 7   PKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPGSANTML 66
           PK K RPIVRLGIFLI+HS  FSV+  +AGVLALLLLP+ A NTYISENALMPGSA +ML
Sbjct: 19  PKIKPRPIVRLGIFLIAHSPVFSVVFSAAGVLALLLLPLLAKNTYISENALMPGSARSML 78

Query: 67  SGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSSQFHPLH 126
           S ++VS+ + LVKD+K         +   Q+++ +Y+S++GAEV+Y +F P+ +QFHPLH
Sbjct: 79  SNRDVSDGSKLVKDIKNFRLNHEGQV---QKLIGKYMSDMGAEVSYQKFHPEGNQFHPLH 138

Query: 127 FFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHETLSLGIA 186
           FFS PDS  + +NVSC + G+N  GIIRAP+ DGKE+IVLVTPY+ +    +E LSLGI 
Sbjct: 139 FFSGPDSYTLLENVSCASYGVNVAGIIRAPRGDGKESIVLVTPYDFINGGDYEDLSLGIV 198

Query: 187 YSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYTCAETNV 246
            S+FSLL++V+WL+KDI+WL ADSRYG+Y  V+AWL +YH+PSF  S+++    C E N 
Sbjct: 199 SSLFSLLSRVTWLSKDIIWLVADSRYGDYRPVAAWLTEYHSPSFKVSDLL---KCDEQN- 258

Query: 247 LYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLI 306
                        + D+F+RAGTMAAALV+KV  RSE FED+LS+YAEASNGQMPNLDLI
Sbjct: 259 -------------TADNFRRAGTMAAALVLKVDGRSEKFEDTLSIYAEASNGQMPNLDLI 318

Query: 307 NIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGMLASDYV 366
           N+VNYLA HRQGF +K+EK   LL   WLK  GE FE++GK    LNP+W FG+ A+DY+
Sbjct: 319 NVVNYLAVHRQGFYVKVEKVVSLLSSSWLKIFGEIFEAVGKLAHMLNPDWNFGIPAADYL 378

Query: 367 DGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFILRGGRLI 426
           +G+ATLASSLY QA+GIPTG HGAFRDYQ+DAIT+++SP+F    K R+ +F LRG RL+
Sbjct: 379 EGSATLASSLYSQALGIPTGPHGAFRDYQVDAITLKVSPRFPPDSKTRQHDFFLRGARLL 438

Query: 427 EGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLYSYANNS 486
           EG +RSVNNLLEKFHQSFFLY++ S SKF+SVGVYMIAFALLVAPLP VAASLY     S
Sbjct: 439 EGTIRSVNNLLEKFHQSFFLYMLTSPSKFISVGVYMIAFALLVAPLPMVAASLYIDGCKS 498

Query: 487 NLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQIPG-YS 546
            LT          NS      + +SWKWL+AAK+VF +HL G +V+LLPYFI Q+PG +S
Sbjct: 499 -LT----------NSTHNPTENFKSWKWLDAAKQVFTLHLLGFIVTLLPYFICQVPGQHS 558

Query: 547 PTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGLCLMSV 606
           PT  SI+W   S   LLI  V +  P  S  S       WA LK++T SAAFIGLCLMS+
Sbjct: 559 PTNRSIMWGTTSSSLLLITFVTM--PGCSPFSSRLHGTNWAILKSVTISAAFIGLCLMSI 618

Query: 607 INFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFFLFKAA 666
           INFATAE+GA L+V  CL+A P+K  L +   K+L  A  ++VL  I FP + F + K  
Sbjct: 619 INFATAEIGALLLVPTCLMAQPIKPALRSRRLKSLLGAFCSIVLLTIGFPVMFFAISKGL 678

Query: 667 L-QGFDNLHL-GDFWNWMETLWAWNSATFLYLGMVHLPCWVLCTQILLHP 714
           L +G   L L G+FW W+E+LWAW SAT+LY+GMVHLPCW+LC  IL HP
Sbjct: 679 LGEGLVGLSLGGEFWTWLESLWAWKSATYLYIGMVHLPCWLLCLCILFHP 695

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O432923.1e-2424.90Glycosylphosphatidylinositol anchor attachment 1 protein OS=Homo sapiens OX=9606... [more]
Q9WTK37.7e-2323.51Glycosylphosphatidylinositol anchor attachment 1 protein OS=Mus musculus OX=1009... [more]
Q9US481.5e-1823.59GPI transamidase component gaa1 OS=Schizosaccharomyces pombe (strain 972 / ATCC ... [more]
P390123.8e-1422.65GPI transamidase component GAA1 OS=Saccharomyces cerevisiae (strain ATCC 204508 ... [more]
Match NameE-valueIdentityDescription
A0A6J1K1W50.0e+00100.00glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucu... [more]
A0A6J1FHQ40.0e+0097.76glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucu... [more]
A0A6J1JZW50.0e+00100.00glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X2 OS=Cucu... [more]
A0A6J1FMH60.0e+0097.76glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X2 OS=Cucu... [more]
A0A0A0KP050.0e+0091.88Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523060 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022993328.10.0e+00100.00glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 [Cucurb... [more]
XP_023550991.10.0e+0098.46glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 [Cucurb... [more]
KAG7016207.10.0e+0097.90Glycosylphosphatidylinositol anchor attachment 1 protein [Cucurbita argyrosperma... [more]
XP_022939757.10.0e+0097.76glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 [Cucurb... [more]
KAG6578667.10.0e+0097.70Glycosylphosphatidylinositol anchor attachment 1 protein, partial [Cucurbita arg... [more]
Match NameE-valueIdentityDescription
AT5G19130.15.7e-22357.89GPI transamidase component family protein / Gaa1-like family protein [more]
AT5G19130.29.8e-22357.89GPI transamidase component family protein / Gaa1-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007246GPI transamidase component Gaa1PIRSFPIRSF036762GAA1coord: 1..714
e-value: 1.6E-197
score: 655.7
IPR007246GPI transamidase component Gaa1PFAMPF04114Gaa1coord: 145..706
e-value: 6.3E-109
score: 365.2
IPR007246GPI transamidase component Gaa1PANTHERPTHR13304GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEINcoord: 1..711
NoneNo IPR availablePANTHERPTHR13304:SF0GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEINcoord: 1..711

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh15G003580.1CmaCh15G003580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016255 attachment of GPI anchor to protein
cellular_component GO:0042765 GPI-anchor transamidase complex
cellular_component GO:0016021 integral component of membrane