CmaCh15G003330 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh15G003330
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionRNA exonuclease 4
LocationCma_Chr15: 1535048 .. 1537474 (-)
RNA-Seq ExpressionCmaCh15G003330
SyntenyCmaCh15G003330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AGCTTCCTTTGCACCCACTTTCTGGCATCAAAGTAAGAGTTTTTGTTTGATCAGACAATGGATCATTCAGCTGAGACATCTAGGTTTGATCTCCTTCTCCCTGTCTTTGTGTATTTGTTTGTGGCATTGTTTCTAAGTTTTTGACACTAAAAATGGTTGTCAAACAGGCACAAGTGCGCTGCTTGCTTCAGGCAATTCAACAAGATTGAGCATTTGGTTGATCATATGAGGACCTCTTTCCACTCACCTCATGAACCCACTTGTGCCGTTTGCAAGAAACACTGTCGTTCTCTTGATTCTCTTAGAGAACATCTCATTGGTATGTTAGTCGTTTCTACCGATAAAGTCGTAAGCTTTTTAAAGTTCAATAAAAGTTCGTATATAAGACCTATAGTGAGATCCCACATCGGTTGGAGAGGGGAACGAAACATTTCTTATAAGAGTGTGGAAACCTCTCTCTAGCAGATGCGTTTTAAAACCGTTAGGTTGACGACGATATGTAACGAACTAAAACGGACAATATCGACTAGCAGTGGCCTTGGGCTATTACAAATGGTATTAGAGCCTGTGTGCCAGCGAGAACGCTGGCCCCCAAGGGAGATGGATTATGAAATCCCATATCGGTTGGAGAGGGAAACAAAACATTTCTTGTAAAGGTGTGGAAACCTCTCCCTAGAAAACGCATTTTAAAATCGTGAGGCTGACGACGATACGTAACATGCCAAAGCGGACAATATCTCCTAACAGTGGGCTTGAGTTGTTACAAATGGTATTAGAGTTAGACACTGTGCTAGTGAGGAAGCTAGCACCCAGAGAGGGTGGATTGTGAGATCCTACATCGGTTGGAGAGGGAAACGAAGCATTCATTGTAAGGGTGTGGAAACTTCTCTCTAGTAGATGCATTTTAAAATCGTGAAGCTAACGACGATACATAACGAGTCAAAGTGGACAATATTTGTCAGCAGTAGACTTGAACTATTACATATACTATAATGGGTTATGATAAATTTAATTATATCGATACGATCATGTTGTTATCAGGGCCGCTGCCGAAGCAAGAATGCAAAAACGTCTTCGACATGCGAGGATGCAAGTTTTGCTTGGCCATCCTTGACAGTTCTTACTCACAAAGGCTCCACCAAGAAAGATGCCAATTCTCGTGTGTTAATTCTGTATGAACCCTCAACTATCAGCTAATTAAACAGCATGAAATTTAGTTCTTTACCTTCAAAATCTTGAATCTTCCATTCTTTTTTGGCTGTTTTGATCAGGGGCTTCTTGCTCGTTTGGCCAACTTGGGAATTCGCGATGGTTCAACCGCCATCGACGGTGGAAGAACTCGAGGCTCCGGTCCCGTGGCGTTAGCTTGCAAATATGTAGGTGGCGGTGATGGGTCGCTGGATGTCTGTGCCAAGGTCTGCCTTATTGATGAGAGTGAAAATGTCATCTTCTATAGCTATGTCAAGCCCACAATACCCGTTACAAATTATAGGTCAGTTATTTTTCGTGGCGAATCATTTAAAATTTAGAGACCAAATAAACCTAAAGTTTTAGAATTGGAGTATAAATTTTTTATAATTTAACCGCTTTGATATGTAAAAATAAAATTAGGTATGAAACGACGGGCATTCGTCCCGAACATGTTCGAGATGCAATGCCACTAAAGCAAGTTCAAAAGAAAATTCAAGACATCCTCTACAATGGAGGTGAGAAAGCTAGGGTTCTTGTGGGTCACTGTGTGGAAGACGATCTCAAACGTCTACAAATCGGATATCCATCTATCATGATAAGGTAAATTCTTCAAACAAAAATTAAATTTATCGTGACCCATCAACTCAAAATTTTAAATTTCTTATCGAAATTTGAAGTTCTAACCTTATATCAACTTTTTTACGAAACCCTTTTAAGATCTTGACCTAAAAGTGAATAGTTCTAAAGATTGCTTGTGTTTCATGCACTGATCCACAGGGACATAGCAAATTATCCGCCATTGATGAAGACAAGCAAGCTCTGCAATTCACTCAAGTATCTAGCTCAAGTATATCTTGGGCAAGTACCATTTTCAATTCTAGTACTCGTTAGTATACTTCATTCAAAAACGAACACGATTTTCAAAAAATAACATACGAAATGATTATCGAGCAAGGTTTATGTAATAAACGAATAATTGGTATTTGTTATTGTAGATTTGAGATTCAAAATGGAGTACAAGATCCTTATGAAGAATGTGTGGGTGGCATGAGATTGTACAAGAGAATGAGATCACAAGTTCATAGGATAGAGCATCATCCAATGGCTTCTGACCCACAAAACAAAAACAACTTTGCAGCTTGGAGGGAAAGTGAGCTTGAGAGGATGAGCCCAGAACAGATGTTGGAGATTTCTAGGTCTGATTATTACTGTTGGTGCATGGATTTGAGGATTTGA

mRNA sequence

AGCTTCCTTTGCACCCACTTTCTGGCATCAAAGTAAGAGTTTTTGTTTGATCAGACAATGGATCATTCAGCTGAGACATCTAGGCACAAGTGCGCTGCTTGCTTCAGGCAATTCAACAAGATTGAGCATTTGGTTGATCATATGAGGACCTCTTTCCACTCACCTCATGAACCCACTTGTGCCGTTTGCAAGAAACACTGTCGTTCTCTTGATTCTCTTAGAGAACATCTCATTGGGCCGCTGCCGAAGCAAGAATGCAAAAACGTCTTCGACATGCGAGGATGCAAGTTTTGCTTGGCCATCCTTGACAGTTCTTACTCACAAAGGCTCCACCAAGAAAGATGCCAATTCTCGTGTGTTAATTCTGGGCTTCTTGCTCGTTTGGCCAACTTGGGAATTCGCGATGGTTCAACCGCCATCGACGGTGGAAGAACTCGAGGCTCCGGTCCCGTGGCGTTAGCTTGCAAATATGTAGGTGGCGGTGATGGGTCGCTGGATGTCTGTGCCAAGGTCTGCCTTATTGATGAGAGTGAAAATGTCATCTTCTATAGCTATGTCAAGCCCACAATACCCGTTACAAATTATAGGTATGAAACGACGGGCATTCGTCCCGAACATGTTCGAGATGCAATGCCACTAAAGCAAGTTCAAAAGAAAATTCAAGACATCCTCTACAATGGAGGTGAGAAAGCTAGGGTTCTTGTGGGTCACTGTGTGGAAGACGATCTCAAACGTCTACAAATCGGATATCCATCTATCATGATAAGGGACATAGCAAATTATCCGCCATTGATGAAGACAAGCAAGCTCTGCAATTCACTCAAATTTGAGATTCAAAATGGAGTACAAGATCCTTATGAAGAATGTGTGGGTGGCATGAGATTGTACAAGAGAATGAGATCACAAGTTCATAGGATAGAGCATCATCCAATGGCTTCTGACCCACAAAACAAAAACAACTTTGCAGCTTGGAGGGAAAGTGAGCTTGAGAGGATGAGCCCAGAACAGATGTTGGAGATTTCTAGGTCTGATTATTACTGTTGGTGCATGGATTTGAGGATTTGA

Coding sequence (CDS)

ATGGATCATTCAGCTGAGACATCTAGGCACAAGTGCGCTGCTTGCTTCAGGCAATTCAACAAGATTGAGCATTTGGTTGATCATATGAGGACCTCTTTCCACTCACCTCATGAACCCACTTGTGCCGTTTGCAAGAAACACTGTCGTTCTCTTGATTCTCTTAGAGAACATCTCATTGGGCCGCTGCCGAAGCAAGAATGCAAAAACGTCTTCGACATGCGAGGATGCAAGTTTTGCTTGGCCATCCTTGACAGTTCTTACTCACAAAGGCTCCACCAAGAAAGATGCCAATTCTCGTGTGTTAATTCTGGGCTTCTTGCTCGTTTGGCCAACTTGGGAATTCGCGATGGTTCAACCGCCATCGACGGTGGAAGAACTCGAGGCTCCGGTCCCGTGGCGTTAGCTTGCAAATATGTAGGTGGCGGTGATGGGTCGCTGGATGTCTGTGCCAAGGTCTGCCTTATTGATGAGAGTGAAAATGTCATCTTCTATAGCTATGTCAAGCCCACAATACCCGTTACAAATTATAGGTATGAAACGACGGGCATTCGTCCCGAACATGTTCGAGATGCAATGCCACTAAAGCAAGTTCAAAAGAAAATTCAAGACATCCTCTACAATGGAGGTGAGAAAGCTAGGGTTCTTGTGGGTCACTGTGTGGAAGACGATCTCAAACGTCTACAAATCGGATATCCATCTATCATGATAAGGGACATAGCAAATTATCCGCCATTGATGAAGACAAGCAAGCTCTGCAATTCACTCAAATTTGAGATTCAAAATGGAGTACAAGATCCTTATGAAGAATGTGTGGGTGGCATGAGATTGTACAAGAGAATGAGATCACAAGTTCATAGGATAGAGCATCATCCAATGGCTTCTGACCCACAAAACAAAAACAACTTTGCAGCTTGGAGGGAAAGTGAGCTTGAGAGGATGAGCCCAGAACAGATGTTGGAGATTTCTAGGTCTGATTATTACTGTTGGTGCATGGATTTGAGGATTTGA

Protein sequence

MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIGPLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTAIDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYETTGIRPEHVRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIANYPPLMKTSKLCNSLKFEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPMASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMDLRI
Homology
BLAST of CmaCh15G003330 vs. ExPASy Swiss-Prot
Match: Q91560 (RNA exonuclease 4 OS=Xenopus laevis OX=8355 GN=rexo4 PE=2 SV=2)

HSP 1 Score: 96.3 bits (238), Expect = 7.1e-19
Identity = 58/156 (37.18%), Postives = 82/156 (52.56%), Query Frame = 0

Query: 132 VALACKYVG-GGDGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYETTGIRPEHVRD 191
           VA+ C+ VG G DG   + A+V +++     ++  YVKPT  VT+YR   +GIRPE V+ 
Sbjct: 235 VAMDCEMVGVGMDGEESILARVSIVNLFGKCVYDKYVKPTERVTDYRTAVSGIRPEDVKK 294

Query: 192 AMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIANYPPLMKTSK 251
             P K VQK++ +IL     + R LVGH V +DLK L + +P   IRD   Y P  +  K
Sbjct: 295 GEPFKVVQKEVSEIL-----RGRTLVGHAVHNDLKILFLDHPKKAIRDTQKYKPFKQKVK 354

Query: 252 --------LCNS-LKFEIQNGVQDPYEECVGGMRLY 278
                   LC   L  ++Q G     ++    MRLY
Sbjct: 355 SGRPSLKLLCEKILNVKVQTGEHCSVQDAQAAMRLY 385

BLAST of CmaCh15G003330 vs. ExPASy Swiss-Prot
Match: Q08237 (RNA exonuclease 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=REX4 PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 6.0e-18
Identity = 55/184 (29.89%), Postives = 98/184 (53.26%), Query Frame = 0

Query: 119 TAIDGGRTRGSGP-VALACKYVG-GGDGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNY 178
           T I+  +++  G  +A+ C++VG G +G     A++ +++   +V+   +VKP   V  +
Sbjct: 108 TRINSNKSKEIGKYIAMDCEFVGVGPEGKESALARISIVNYFGHVVLDEFVKPREKVVEW 167

Query: 179 RYETTGIRPEHVRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMI 238
           R   +GI+PEH+++A+  K+ QKK  DIL     + R+LVGH ++ DL+ L + +P  ++
Sbjct: 168 RTWVSGIKPEHMKNAITFKEAQKKTADIL-----EGRILVGHALKHDLEALMLSHPKSLL 227

Query: 239 RDIANYPPLMK---------TSKLCNS-LKFEIQNGVQDPYEECVGGMRLYKRMRSQVHR 291
           RD + + P  K           KL    LK  IQ G     E+    M LYK+ +++  +
Sbjct: 228 RDTSRHLPFRKLYAKGKTPSLKKLTREVLKISIQEGEHSSVEDARATMLLYKKEKTEFEK 286

BLAST of CmaCh15G003330 vs. ExPASy Swiss-Prot
Match: Q9GZR2 (RNA exonuclease 4 OS=Homo sapiens OX=9606 GN=REXO4 PE=1 SV=2)

HSP 1 Score: 91.7 bits (226), Expect = 1.7e-17
Identity = 62/206 (30.10%), Postives = 102/206 (49.51%), Query Frame = 0

Query: 112 LGIRDGSTAID-------GGRTRGSGPVALACKYVG-GGDGSLDVCAKVCLIDESENVIF 171
           LG  +GS ++        GG TR    +AL C+ VG G  G   + A+V ++++    ++
Sbjct: 220 LGQSEGSVSLSLVKEQAFGGLTRA---LALDCEMVGVGPKGEESMAARVSIVNQYGKCVY 279

Query: 172 YSYVKPTIPVTNYRYETTGIRPEHVRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDD 231
             YVKPT PVT+YR   +GIRPE+++    L+ VQK++ ++L     K R+LVGH + +D
Sbjct: 280 DKYVKPTEPVTDYRTAVSGIRPENLKQGEELEVVQKEVAEML-----KGRILVGHALHND 339

Query: 232 LKRLQIGYPSIMIRDIANYPPLMKTSKLCNS---------LKFEIQNGVQDPYEECVGGM 291
           LK L + +P   IRD   Y P     K             L  ++Q       ++    M
Sbjct: 340 LKVLFLDHPKKKIRDTQKYKPFKSQVKSGRPSLRLLSEKILGLQVQQAEHCSIQDAQAAM 399

Query: 292 RLYKRMRSQVHRI--EHHPMASDPQN 299
           RLY  ++ +   +  +  P+ + P +
Sbjct: 400 RLYVMVKKEWESMARDRRPLLTAPDH 417

BLAST of CmaCh15G003330 vs. ExPASy Swiss-Prot
Match: Q6CMT3 (RNA exonuclease 4 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=REX4 PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.3e-17
Identity = 57/179 (31.84%), Postives = 95/179 (53.07%), Query Frame = 0

Query: 125 RTRGSGP-VALACKYVG-GGDGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYETTG 184
           RT G G  V++ C++VG G DG     A+V +++   NV+   +V+P  PVT++R   +G
Sbjct: 112 RTNGVGKYVSMDCEFVGVGPDGKDSALARVSIVNYYGNVVLDLFVRPKEPVTDWRTWVSG 171

Query: 185 IRPEHVRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIANY 244
           I+P H+ +A+  +  QK++ ++L     K R+LVGH V  DL  L + +P  MIRD + +
Sbjct: 172 IKPHHMANAVTQEDCQKQVSNVL-----KGRILVGHSVHHDLTALMLSHPRRMIRDTSRH 231

Query: 245 ------------PPLMKTSKLCNSLKFEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEH 290
                       P L K +K    L+ +IQ+G     E+    M LYK  + +  ++ +
Sbjct: 232 MPFRQKYSEGKTPSLKKLTK--EILQLDIQDGEHSSIEDARATMLLYKSDKLEFEKLHN 283

BLAST of CmaCh15G003330 vs. ExPASy Swiss-Prot
Match: Q4WHF8 (RNA exonuclease 4 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=rex4 PE=3 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 8.6e-17
Identity = 57/169 (33.73%), Postives = 90/169 (53.25%), Query Frame = 0

Query: 132 VALACKYVGGGDG--SLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYETTGIRPEHVR 191
           VA+ C+ VG G    +    A+V +++ +   ++ SYV+P   +T++R   +GI P+H+ 
Sbjct: 117 VAMDCEMVGVGPNPDNDSALARVSIVNFNGEQVYDSYVRPKEMITDWRTHVSGISPKHMA 176

Query: 192 DAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIANYPPLMKTS 251
           +A  L+QVQK + +IL       R+LVGH V +DL  L +G+P   IRD + +PP  K +
Sbjct: 177 EARSLEQVQKDVAEIL-----DGRILVGHAVSNDLDALLLGHPKRDIRDTSKHPPYRKIA 236

Query: 252 -------KLCNS--LKFEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEH 290
                  K+  S  L   IQ+G     E+    M LY+R +    R EH
Sbjct: 237 GGGSPRLKILASEFLGLNIQDGAHSSVEDAKATMLLYRRDKEAFER-EH 279

BLAST of CmaCh15G003330 vs. ExPASy TrEMBL
Match: A0A6J1JZ30 (RNA exonuclease 4 OS=Cucurbita maxima OX=3661 GN=LOC111489566 PE=3 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 1.1e-197
Identity = 335/343 (97.67%), Postives = 335/343 (97.67%), Query Frame = 0

Query: 1   MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG 60
           MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG
Sbjct: 1   MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG 60

Query: 61  PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA 120
           PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA
Sbjct: 61  PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA 120

Query: 121 IDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYET 180
           IDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYET
Sbjct: 121 IDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYET 180

Query: 181 TGIRPEHVRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIA 240
           TGIRPEHVRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIA
Sbjct: 181 TGIRPEHVRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIA 240

Query: 241 NYPPLMKTSKLCNSLK--------FEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPM 300
           NYPPLMKTSKLCNSLK        FEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPM
Sbjct: 241 NYPPLMKTSKLCNSLKYLAQVYLGFEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPM 300

Query: 301 ASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMDLRI 336
           ASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMDLRI
Sbjct: 301 ASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMDLRI 343

BLAST of CmaCh15G003330 vs. ExPASy TrEMBL
Match: A0A6J1FEK0 (RNA exonuclease 4 OS=Cucurbita moschata OX=3662 GN=LOC111444794 PE=3 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 6.3e-196
Identity = 332/343 (96.79%), Postives = 333/343 (97.08%), Query Frame = 0

Query: 1   MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG 60
           MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG
Sbjct: 1   MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG 60

Query: 61  PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA 120
           PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA
Sbjct: 61  PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA 120

Query: 121 IDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYET 180
           IDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESENVIFYSYVKP IPVTNYRYE 
Sbjct: 121 IDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESENVIFYSYVKPIIPVTNYRYEM 180

Query: 181 TGIRPEHVRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIA 240
           TGIRPEH+RDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIA
Sbjct: 181 TGIRPEHLRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIA 240

Query: 241 NYPPLMKTSKLCNSLK--------FEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPM 300
           NYPPLMKTSKLCNSLK        FEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPM
Sbjct: 241 NYPPLMKTSKLCNSLKYLAQVYLGFEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPM 300

Query: 301 ASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMDLRI 336
           ASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMDLRI
Sbjct: 301 ASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMDLRI 343

BLAST of CmaCh15G003330 vs. ExPASy TrEMBL
Match: A0A6J1BXL4 (RNA exonuclease 4 OS=Momordica charantia OX=3673 GN=LOC111006633 PE=3 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 4.6e-170
Identity = 290/344 (84.30%), Postives = 310/344 (90.12%), Query Frame = 0

Query: 1   MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG 60
           MDH  ETSRHKCAACFRQFNKIEHLVDHMRTSFHS HEP CA+CKKHCRSLDSLREHLIG
Sbjct: 1   MDHPLETSRHKCAACFRQFNKIEHLVDHMRTSFHSVHEPICAICKKHCRSLDSLREHLIG 60

Query: 61  PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA 120
           PLPKQECKN+F  RGCKFCLAILDS YSQRLHQERCQFS VNSGLLARLANLGIRD S  
Sbjct: 61  PLPKQECKNIFTTRGCKFCLAILDSPYSQRLHQERCQFSGVNSGLLARLANLGIRDASIV 120

Query: 121 IDGGRTRGSGPVALACKYV-GGGDGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYE 180
           +DGGR R +G VALACKYV GGGDGSLD+CAKVCLIDE+ENVIFYSYVKP IPVTNYR+E
Sbjct: 121 VDGGRARATGTVALACKYVGGGGDGSLDICAKVCLIDETENVIFYSYVKPIIPVTNYRFE 180

Query: 181 TTGIRPEHVRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDI 240
           TTGIRPEH+RDAMPLKQVQKKIQ+ILYNGGEKARVLVGH VEDDLKRLQIGYP  +IRD 
Sbjct: 181 TTGIRPEHLRDAMPLKQVQKKIQEILYNGGEKARVLVGHGVEDDLKRLQIGYP--IIRDT 240

Query: 241 ANYPPLMKTSKLCNSLK--------FEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHP 300
           ANYPPLMKTSKLCNSLK        F+IQNG+QDPYE+CV  MRLYKRMRSQVH+IE +P
Sbjct: 241 ANYPPLMKTSKLCNSLKYLAQVYLGFDIQNGIQDPYEDCVASMRLYKRMRSQVHKIEDYP 300

Query: 301 MASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMDLRI 336
           MASDPQNKNNF  WR++ELERM+PE ML+ISRSDYYCWC+DLRI
Sbjct: 301 MASDPQNKNNFGIWRQNELERMTPEHMLDISRSDYYCWCLDLRI 342

BLAST of CmaCh15G003330 vs. ExPASy TrEMBL
Match: A0A6J1BZE0 (RNA exonuclease 4 OS=Momordica charantia OX=3673 GN=LOC111006633 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.9e-168
Identity = 290/347 (83.57%), Postives = 310/347 (89.34%), Query Frame = 0

Query: 1   MDHSAETS---RHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREH 60
           MDH  ETS   RHKCAACFRQFNKIEHLVDHMRTSFHS HEP CA+CKKHCRSLDSLREH
Sbjct: 1   MDHPLETSRSVRHKCAACFRQFNKIEHLVDHMRTSFHSVHEPICAICKKHCRSLDSLREH 60

Query: 61  LIGPLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDG 120
           LIGPLPKQECKN+F  RGCKFCLAILDS YSQRLHQERCQFS VNSGLLARLANLGIRD 
Sbjct: 61  LIGPLPKQECKNIFTTRGCKFCLAILDSPYSQRLHQERCQFSGVNSGLLARLANLGIRDA 120

Query: 121 STAIDGGRTRGSGPVALACKYV-GGGDGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNY 180
           S  +DGGR R +G VALACKYV GGGDGSLD+CAKVCLIDE+ENVIFYSYVKP IPVTNY
Sbjct: 121 SIVVDGGRARATGTVALACKYVGGGGDGSLDICAKVCLIDETENVIFYSYVKPIIPVTNY 180

Query: 181 RYETTGIRPEHVRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMI 240
           R+ETTGIRPEH+RDAMPLKQVQKKIQ+ILYNGGEKARVLVGH VEDDLKRLQIGYP  +I
Sbjct: 181 RFETTGIRPEHLRDAMPLKQVQKKIQEILYNGGEKARVLVGHGVEDDLKRLQIGYP--II 240

Query: 241 RDIANYPPLMKTSKLCNSLK--------FEIQNGVQDPYEECVGGMRLYKRMRSQVHRIE 300
           RD ANYPPLMKTSKLCNSLK        F+IQNG+QDPYE+CV  MRLYKRMRSQVH+IE
Sbjct: 241 RDTANYPPLMKTSKLCNSLKYLAQVYLGFDIQNGIQDPYEDCVASMRLYKRMRSQVHKIE 300

Query: 301 HHPMASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMDLRI 336
            +PMASDPQNKNNF  WR++ELERM+PE ML+ISRSDYYCWC+DLRI
Sbjct: 301 DYPMASDPQNKNNFGIWRQNELERMTPEHMLDISRSDYYCWCLDLRI 345

BLAST of CmaCh15G003330 vs. ExPASy TrEMBL
Match: A0A1S3C947 (RNA exonuclease 4 OS=Cucumis melo OX=3656 GN=LOC103498183 PE=3 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 1.6e-151
Identity = 264/352 (75.00%), Postives = 296/352 (84.09%), Query Frame = 0

Query: 1   MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG 60
           MDHS ETSRHKCAACFRQFNKIEHLVDHMRTSFHS HEPTCA+CKKHCRSLDSLREHLIG
Sbjct: 1   MDHSLETSRHKCAACFRQFNKIEHLVDHMRTSFHSHHEPTCAICKKHCRSLDSLREHLIG 60

Query: 61  PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA 120
           PLPKQEC+N+F  RGCKFCLAILDSSYSQRLHQERCQFS VNSGLLAR ANLGIRDGST 
Sbjct: 61  PLPKQECRNIFATRGCKFCLAILDSSYSQRLHQERCQFSPVNSGLLARFANLGIRDGSTI 120

Query: 121 IDGGRTRGSGPVALACKYVGGG-DGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYE 180
           ID GRTRGSG +ALACKYVGGG DGSLD+CAKVCL+DE ENVIFYSYVKP IPVTNYRYE
Sbjct: 121 IDSGRTRGSGAIALACKYVGGGSDGSLDICAKVCLVDEGENVIFYSYVKPIIPVTNYRYE 180

Query: 181 TTGIRPEHVRDAMPLKQVQKKIQDILYNGGE----------KARVLVGHCVEDDLKRLQI 240
            TG+RPEH+RDAMPLKQVQKKIQ+ L NG +          KAR+LVGH +++ L  LQI
Sbjct: 181 FTGVRPEHLRDAMPLKQVQKKIQEFLCNGEQMWKIRPGSTRKARILVGHGLQEYLTSLQI 240

Query: 241 GYPSIMIRDIANYPPLMKTSKLCNSLK--------FEIQNGVQ-DPYEECVGGMRLYKRM 300
            YPSIMIRD A YPPLMKTSKL NSL         + ++NG + + YEECV  MRLY +M
Sbjct: 241 DYPSIMIRDTAKYPPLMKTSKLSNSLHYLTQTYLGYNMENGKKSEGYEECVATMRLYLKM 300

Query: 301 RSQVHRIEHHPMASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMD 333
           +SQVH+ E +P+ASDP NK NFA W++ ELE+M+P+Q+L+ISRSDYYCWC+D
Sbjct: 301 KSQVHKREEYPLASDPPNKFNFAIWKQLELEKMTPQQLLQISRSDYYCWCLD 352

BLAST of CmaCh15G003330 vs. NCBI nr
Match: XP_022993635.1 (RNA exonuclease 4-like [Cucurbita maxima])

HSP 1 Score: 698.7 bits (1802), Expect = 2.4e-197
Identity = 335/343 (97.67%), Postives = 335/343 (97.67%), Query Frame = 0

Query: 1   MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG 60
           MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG
Sbjct: 1   MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG 60

Query: 61  PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA 120
           PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA
Sbjct: 61  PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA 120

Query: 121 IDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYET 180
           IDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYET
Sbjct: 121 IDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYET 180

Query: 181 TGIRPEHVRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIA 240
           TGIRPEHVRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIA
Sbjct: 181 TGIRPEHVRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIA 240

Query: 241 NYPPLMKTSKLCNSLK--------FEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPM 300
           NYPPLMKTSKLCNSLK        FEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPM
Sbjct: 241 NYPPLMKTSKLCNSLKYLAQVYLGFEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPM 300

Query: 301 ASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMDLRI 336
           ASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMDLRI
Sbjct: 301 ASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMDLRI 343

BLAST of CmaCh15G003330 vs. NCBI nr
Match: XP_022938612.1 (RNA exonuclease 4-like [Cucurbita moschata])

HSP 1 Score: 693.0 bits (1787), Expect = 1.3e-195
Identity = 332/343 (96.79%), Postives = 333/343 (97.08%), Query Frame = 0

Query: 1   MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG 60
           MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG
Sbjct: 1   MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG 60

Query: 61  PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA 120
           PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA
Sbjct: 61  PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA 120

Query: 121 IDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYET 180
           IDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESENVIFYSYVKP IPVTNYRYE 
Sbjct: 121 IDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESENVIFYSYVKPIIPVTNYRYEM 180

Query: 181 TGIRPEHVRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIA 240
           TGIRPEH+RDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIA
Sbjct: 181 TGIRPEHLRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIA 240

Query: 241 NYPPLMKTSKLCNSLK--------FEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPM 300
           NYPPLMKTSKLCNSLK        FEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPM
Sbjct: 241 NYPPLMKTSKLCNSLKYLAQVYLGFEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPM 300

Query: 301 ASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMDLRI 336
           ASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMDLRI
Sbjct: 301 ASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMDLRI 343

BLAST of CmaCh15G003330 vs. NCBI nr
Match: XP_023551468.1 (RNA exonuclease 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 678.7 bits (1750), Expect = 2.5e-191
Identity = 325/343 (94.75%), Postives = 332/343 (96.79%), Query Frame = 0

Query: 1   MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG 60
           MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG
Sbjct: 1   MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG 60

Query: 61  PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA 120
           PLPKQECKNVFDMRGCKFCLAILDSSYSQR+HQERCQFSCVNSGLLARLA+LGIRDGSTA
Sbjct: 61  PLPKQECKNVFDMRGCKFCLAILDSSYSQRVHQERCQFSCVNSGLLARLASLGIRDGSTA 120

Query: 121 IDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYET 180
           IDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESEN++FYSYVKP IPVTNYRYET
Sbjct: 121 IDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESENIVFYSYVKPIIPVTNYRYET 180

Query: 181 TGIRPEHVRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIA 240
           TGIRPEH+RDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIA
Sbjct: 181 TGIRPEHLRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIA 240

Query: 241 NYPPLMKTSKLCNSLK--------FEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPM 300
           NYPPLMKTSKLCNSLK        FEIQNGVQD Y +CVGGMRLYKRMRSQVHRIEHHPM
Sbjct: 241 NYPPLMKTSKLCNSLKYLAQVYLGFEIQNGVQDLY-DCVGGMRLYKRMRSQVHRIEHHPM 300

Query: 301 ASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMDLRI 336
           ASDPQNKNNFAAWRE+ELERMSPEQMLEISRSDYYCWCMDLRI
Sbjct: 301 ASDPQNKNNFAAWRENELERMSPEQMLEISRSDYYCWCMDLRI 342

BLAST of CmaCh15G003330 vs. NCBI nr
Match: KAG6578645.1 (hypothetical protein SDJN03_23093, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 647.5 bits (1669), Expect = 6.3e-182
Identity = 312/335 (93.13%), Postives = 314/335 (93.73%), Query Frame = 0

Query: 1   MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG 60
           MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG
Sbjct: 1   MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG 60

Query: 61  PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA 120
           PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA
Sbjct: 61  PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA 120

Query: 121 IDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYET 180
           IDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESENVIFYSYVKP IPVTNYRYE 
Sbjct: 121 IDGGRTRGSGPVALACKYVGGGDGSLDVCAKVCLIDESENVIFYSYVKPIIPVTNYRYEM 180

Query: 181 TGIRPEHVRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIA 240
           TGIRPEH+RDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIR   
Sbjct: 181 TGIRPEHLRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIR--- 240

Query: 241 NYPPLMKTSKLCNSLKFEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPMASDPQNKN 300
                           FEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPMASDPQNKN
Sbjct: 241 ----------------FEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPMASDPQNKN 300

Query: 301 NFAAWRESELERMSPEQMLEISRSDYYCWCMDLRI 336
           NFAAWRE+ELERMSPEQMLEISRSDYYCWCMDLRI
Sbjct: 301 NFAAWRENELERMSPEQMLEISRSDYYCWCMDLRI 316

BLAST of CmaCh15G003330 vs. NCBI nr
Match: XP_011656270.1 (RNA exonuclease 4 [Cucumis sativus])

HSP 1 Score: 625.5 bits (1612), Expect = 2.6e-175
Identity = 296/344 (86.05%), Postives = 314/344 (91.28%), Query Frame = 0

Query: 1   MDHSAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIG 60
           MDHS ETSRHKCAACFRQFNKIEHLVDHMRTSFHS HEPTCA+CKKHCRSLDSLREHLIG
Sbjct: 1   MDHSLETSRHKCAACFRQFNKIEHLVDHMRTSFHSHHEPTCAICKKHCRSLDSLREHLIG 60

Query: 61  PLPKQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTA 120
           PLPKQECKN+F  RGCKFCLAI DSSYSQRLHQERCQFS VNSGLLAR ANLGIRDGST 
Sbjct: 61  PLPKQECKNIFATRGCKFCLAIFDSSYSQRLHQERCQFSPVNSGLLARFANLGIRDGSTV 120

Query: 121 IDGGRTRGSGPVALACKYVGGG-DGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYE 180
           ID GRTRGSG +ALACKYVGGG DGS+D+CAKVCLIDE ENVIFYSYVKP   VTNYRYE
Sbjct: 121 IDSGRTRGSGAIALACKYVGGGSDGSMDICAKVCLIDEGENVIFYSYVKPVTAVTNYRYE 180

Query: 181 TTGIRPEHVRDAMPLKQVQKKIQDILYNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDI 240
           TTGIRPEH+RDAMP+KQVQKKIQ+ILYNGGEKAR+LVG CVEDDLKRLQIGYPS MIRD 
Sbjct: 181 TTGIRPEHIRDAMPVKQVQKKIQEILYNGGEKARILVGDCVEDDLKRLQIGYPSFMIRDT 240

Query: 241 ANYPPLMKTSKLCNSLK--------FEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHP 300
           ANYPPLMK+SKLCNSLK        F++QNG+QDPYEEC+  MRLYKRMRSQVHR E++P
Sbjct: 241 ANYPPLMKSSKLCNSLKYLAQVYLGFDLQNGIQDPYEECIVAMRLYKRMRSQVHRKENYP 300

Query: 301 MASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMDLRI 336
           MASDPQNKNNFA WRE+EL+RMSPEQMLEISRSDYYCWCMDLRI
Sbjct: 301 MASDPQNKNNFAIWRENELQRMSPEQMLEISRSDYYCWCMDLRI 344

BLAST of CmaCh15G003330 vs. TAIR 10
Match: AT3G27970.1 (Exonuclease family protein )

HSP 1 Score: 476.5 bits (1225), Expect = 1.8e-134
Identity = 227/347 (65.42%), Postives = 278/347 (80.12%), Query Frame = 0

Query: 4   SAETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIGPLP 63
           S+ET R+KCAAC+RQFNK+EHLV+HM+ S+HS HEPTC VCKKHCRS +SLREHLIGPLP
Sbjct: 9   SSETLRNKCAACYRQFNKLEHLVEHMKISYHSGHEPTCGVCKKHCRSFESLREHLIGPLP 68

Query: 64  KQECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTAIDG 123
           KQECKN+F +RGC+FC+ IL+S  S+R+HQERCQFS VNSGL  R+A LG+RD    ID 
Sbjct: 69  KQECKNIFSLRGCRFCMTILESPNSRRIHQERCQFSSVNSGLTTRMAALGLRD-KAMIDY 128

Query: 124 GRTRGSGPVALACKYVGGG-DGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYETTG 183
             +R    VAL+CK VGGG DGSLD+CA+VC+ DES+NVIF++YVKP++ VT+YRYETTG
Sbjct: 129 TSSRSPRVVALSCKMVGGGSDGSLDLCARVCITDESDNVIFHTYVKPSMAVTSYRYETTG 188

Query: 184 IRPEHVRDAMPLKQVQKKIQDILYNG---------GEKARVLVGHCVEDDLKRLQIGYPS 243
           IRPE++RDAMPLKQVQ+KIQ+ L NG         G KAR+LVGH ++ DL RLQ+ YPS
Sbjct: 189 IRPENLRDAMPLKQVQRKIQEFLCNGEPMWKIRPRGGKARILVGHGLDHDLDRLQLEYPS 248

Query: 244 IMIRDIANYPPLMKTSKLCNSLK--------FEIQNGVQDPYEECVGGMRLYKRMRSQVH 303
            MIRD A YPPLMKTSKL NSLK        +++  G+QDPYE+CV  MRLY RMR Q H
Sbjct: 249 SMIRDTAKYPPLMKTSKLSNSLKYLTQAYLGYDVHFGIQDPYEDCVATMRLYTRMRYQKH 308

Query: 304 RIEHHPMASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMD 333
           +IE +P+A+D QN++N  AWR+SE ERMSP++ML ISRSDYYCWC+D
Sbjct: 309 KIEAYPLAADAQNRSNQVAWRQSEAERMSPDEMLSISRSDYYCWCLD 354

BLAST of CmaCh15G003330 vs. TAIR 10
Match: AT5G40310.1 (Exonuclease family protein )

HSP 1 Score: 460.3 bits (1183), Expect = 1.3e-129
Identity = 220/345 (63.77%), Postives = 269/345 (77.97%), Query Frame = 0

Query: 6   ETSRHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIGPLPKQ 65
           +T R+KC  C+RQFNK EHLV+HMR S+HS HEPTC +C KHCRS DSLREHLIGPLPKQ
Sbjct: 2   DTCRNKCGGCYRQFNKKEHLVEHMRISYHSVHEPTCGICNKHCRSFDSLREHLIGPLPKQ 61

Query: 66  ECKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTAIDGGR 125
           ECKN+F +RGC+FCL IL+S  ++R+HQERCQ S V SGL+ R+A LG+R+ ST ID   
Sbjct: 62  ECKNIFSIRGCRFCLTILESPNARRIHQERCQLSNVTSGLMIRMAALGLRNNST-IDYTS 121

Query: 126 TRGSGPVALACKYVGGG-DGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYETTGIR 185
           +R    VAL+CK VGGG DGSLD+CA+VC+ DESENV+F++YVKPTIPVTNYRYE TGIR
Sbjct: 122 SRSPRVVALSCKMVGGGSDGSLDLCARVCITDESENVVFHTYVKPTIPVTNYRYEMTGIR 181

Query: 186 PEHVRDAMPLKQVQKKIQDILYNG---------GEKARVLVGHCVEDDLKRLQIGYPSIM 245
           PE++RDAM LK  Q+K+Q+ L NG           KAR+LVGH +++ L  LQ+ Y S M
Sbjct: 182 PENLRDAMRLKHAQRKVQEFLCNGEPMWKIRPRNGKARILVGHGLDNHLDSLQLEYSSSM 241

Query: 246 IRDIANYPPLMKTSKLCNSLK--------FEIQNGVQDPYEECVGGMRLYKRMRSQVHRI 305
           IRD A YPPLMK+SKL NSLK        ++I  G+QDPYE+CV  MRLY RMR Q HR 
Sbjct: 242 IRDTAEYPPLMKSSKLSNSLKYLTQAYLGYDIHVGIQDPYEDCVATMRLYTRMRYQKHRA 301

Query: 306 EHHPMASDPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMD 333
           E +P+ASD QN NNFAAWR++ELERMSPE++L++SRSDYYCWC+D
Sbjct: 302 EAYPLASDTQNHNNFAAWRQNELERMSPEELLDLSRSDYYCWCLD 345

BLAST of CmaCh15G003330 vs. TAIR 10
Match: AT2G48100.1 (Exonuclease family protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.5e-75
Identity = 142/338 (42.01%), Postives = 210/338 (62.13%), Query Frame = 0

Query: 9   RHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIGP--LPKQE 68
           RH+C AC++ FN+ EHLV+HM+ S+HS H+P C VC KHC+S +S+REHL  P  L K  
Sbjct: 16  RHRCVACYKMFNRREHLVEHMKISYHSLHQPRCGVCLKHCKSFESVREHLNVPDHLSKGN 75

Query: 69  CKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTAIDGGRT 128
           CK +F  RGC  CL I + +++   H+ +C  S         L     R+ S+++ G R 
Sbjct: 76  CKAIFTKRGCTLCLQIFEEAFALAEHKNKCHLSPPRP-----LGTSTQRNPSSSLAGSRL 135

Query: 129 RGSGPVALACKYVGGG-DGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYETTGIRP 188
           +    +AL C+ VGGG DG++D CA VCL+D+ ENVIF ++V+P +PVT+YR+E TG+  
Sbjct: 136 KA---MALDCEMVGGGADGTIDQCASVCLVDDDENVIFSTHVQPLLPVTDYRHEITGLTK 195

Query: 189 EHVRDAMPLKQVQKKIQDIL---YNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIANY 248
           E ++D MPL+ V++++   L    N G    +LVGH +  D+  L++ YPS ++RD A Y
Sbjct: 196 EDLKDGMPLEHVRERVFSFLCGGQNDGAGRLLLVGHDLRHDMSCLKLEYPSHLLRDTAKY 255

Query: 249 PPLMKTSKLCNSLK--------FEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPMAS 308
            PLMKT+ +  SLK        ++IQ G  + YE+CV  MRLYKRMR Q H      + S
Sbjct: 256 VPLMKTNLVSQSLKYLTKSYLGYKIQCGKHEVYEDCVSAMRLYKRMRDQEH------VCS 315

Query: 309 DPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMD 333
                N   + ++S+LE+M+ E++ + S S+Y CWC+D
Sbjct: 316 GKAEGNGLNSRKQSDLEKMNAEELYQKSTSEYRCWCLD 339

BLAST of CmaCh15G003330 vs. TAIR 10
Match: AT2G48100.2 (Exonuclease family protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.5e-75
Identity = 142/338 (42.01%), Postives = 210/338 (62.13%), Query Frame = 0

Query: 9   RHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIGP--LPKQE 68
           RH+C AC++ FN+ EHLV+HM+ S+HS H+P C VC KHC+S +S+REHL  P  L K  
Sbjct: 16  RHRCVACYKMFNRREHLVEHMKISYHSLHQPRCGVCLKHCKSFESVREHLNVPDHLSKGN 75

Query: 69  CKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTAIDGGRT 128
           CK +F  RGC  CL I + +++   H+ +C  S         L     R+ S+++ G R 
Sbjct: 76  CKAIFTKRGCTLCLQIFEEAFALAEHKNKCHLSPPRP-----LGTSTQRNPSSSLAGSRL 135

Query: 129 RGSGPVALACKYVGGG-DGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYETTGIRP 188
           +    +AL C+ VGGG DG++D CA VCL+D+ ENVIF ++V+P +PVT+YR+E TG+  
Sbjct: 136 KA---MALDCEMVGGGADGTIDQCASVCLVDDDENVIFSTHVQPLLPVTDYRHEITGLTK 195

Query: 189 EHVRDAMPLKQVQKKIQDIL---YNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIANY 248
           E ++D MPL+ V++++   L    N G    +LVGH +  D+  L++ YPS ++RD A Y
Sbjct: 196 EDLKDGMPLEHVRERVFSFLCGGQNDGAGRLLLVGHDLRHDMSCLKLEYPSHLLRDTAKY 255

Query: 249 PPLMKTSKLCNSLK--------FEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPMAS 308
            PLMKT+ +  SLK        ++IQ G  + YE+CV  MRLYKRMR Q H      + S
Sbjct: 256 VPLMKTNLVSQSLKYLTKSYLGYKIQCGKHEVYEDCVSAMRLYKRMRDQEH------VCS 315

Query: 309 DPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMD 333
                N   + ++S+LE+M+ E++ + S S+Y CWC+D
Sbjct: 316 GKAEGNGLNSRKQSDLEKMNAEELYQKSTSEYRCWCLD 339

BLAST of CmaCh15G003330 vs. TAIR 10
Match: AT2G48100.3 (Exonuclease family protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.5e-75
Identity = 142/338 (42.01%), Postives = 210/338 (62.13%), Query Frame = 0

Query: 9   RHKCAACFRQFNKIEHLVDHMRTSFHSPHEPTCAVCKKHCRSLDSLREHLIGP--LPKQE 68
           RH+C AC++ FN+ EHLV+HM+ S+HS H+P C VC KHC+S +S+REHL  P  L K  
Sbjct: 16  RHRCVACYKMFNRREHLVEHMKISYHSLHQPRCGVCLKHCKSFESVREHLNVPDHLSKGN 75

Query: 69  CKNVFDMRGCKFCLAILDSSYSQRLHQERCQFSCVNSGLLARLANLGIRDGSTAIDGGRT 128
           CK +F  RGC  CL I + +++   H+ +C  S         L     R+ S+++ G R 
Sbjct: 76  CKAIFTKRGCTLCLQIFEEAFALAEHKNKCHLSPPRP-----LGTSTQRNPSSSLAGSRL 135

Query: 129 RGSGPVALACKYVGGG-DGSLDVCAKVCLIDESENVIFYSYVKPTIPVTNYRYETTGIRP 188
           +    +AL C+ VGGG DG++D CA VCL+D+ ENVIF ++V+P +PVT+YR+E TG+  
Sbjct: 136 KA---MALDCEMVGGGADGTIDQCASVCLVDDDENVIFSTHVQPLLPVTDYRHEITGLTK 195

Query: 189 EHVRDAMPLKQVQKKIQDIL---YNGGEKARVLVGHCVEDDLKRLQIGYPSIMIRDIANY 248
           E ++D MPL+ V++++   L    N G    +LVGH +  D+  L++ YPS ++RD A Y
Sbjct: 196 EDLKDGMPLEHVRERVFSFLCGGQNDGAGRLLLVGHDLRHDMSCLKLEYPSHLLRDTAKY 255

Query: 249 PPLMKTSKLCNSLK--------FEIQNGVQDPYEECVGGMRLYKRMRSQVHRIEHHPMAS 308
            PLMKT+ +  SLK        ++IQ G  + YE+CV  MRLYKRMR Q H      + S
Sbjct: 256 VPLMKTNLVSQSLKYLTKSYLGYKIQCGKHEVYEDCVSAMRLYKRMRDQEH------VCS 315

Query: 309 DPQNKNNFAAWRESELERMSPEQMLEISRSDYYCWCMD 333
                N   + ++S+LE+M+ E++ + S S+Y CWC+D
Sbjct: 316 GKAEGNGLNSRKQSDLEKMNAEELYQKSTSEYRCWCLD 339

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q915607.1e-1937.18RNA exonuclease 4 OS=Xenopus laevis OX=8355 GN=rexo4 PE=2 SV=2[more]
Q082376.0e-1829.89RNA exonuclease 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=55... [more]
Q9GZR21.7e-1730.10RNA exonuclease 4 OS=Homo sapiens OX=9606 GN=REXO4 PE=1 SV=2[more]
Q6CMT32.3e-1731.84RNA exonuclease 4 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 707... [more]
Q4WHF88.6e-1733.73RNA exonuclease 4 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 10... [more]
Match NameE-valueIdentityDescription
A0A6J1JZ301.1e-19797.67RNA exonuclease 4 OS=Cucurbita maxima OX=3661 GN=LOC111489566 PE=3 SV=1[more]
A0A6J1FEK06.3e-19696.79RNA exonuclease 4 OS=Cucurbita moschata OX=3662 GN=LOC111444794 PE=3 SV=1[more]
A0A6J1BXL44.6e-17084.30RNA exonuclease 4 OS=Momordica charantia OX=3673 GN=LOC111006633 PE=3 SV=1[more]
A0A6J1BZE01.9e-16883.57RNA exonuclease 4 OS=Momordica charantia OX=3673 GN=LOC111006633 PE=3 SV=1[more]
A0A1S3C9471.6e-15175.00RNA exonuclease 4 OS=Cucumis melo OX=3656 GN=LOC103498183 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022993635.12.4e-19797.67RNA exonuclease 4-like [Cucurbita maxima][more]
XP_022938612.11.3e-19596.79RNA exonuclease 4-like [Cucurbita moschata][more]
XP_023551468.12.5e-19194.75RNA exonuclease 4-like [Cucurbita pepo subsp. pepo][more]
KAG6578645.16.3e-18293.13hypothetical protein SDJN03_23093, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_011656270.12.6e-17586.05RNA exonuclease 4 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT3G27970.11.8e-13465.42Exonuclease family protein [more]
AT5G40310.11.3e-12963.77Exonuclease family protein [more]
AT2G48100.12.5e-7542.01Exonuclease family protein [more]
AT2G48100.22.5e-7542.01Exonuclease family protein [more]
AT2G48100.32.5e-7542.01Exonuclease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013520Exonuclease, RNase T/DNA polymerase IIISMARTSM00479exoiiienduscoord: 130..286
e-value: 3.0E-11
score: 53.4
IPR013520Exonuclease, RNase T/DNA polymerase IIIPFAMPF00929RNase_Tcoord: 156..238
e-value: 3.5E-6
score: 27.7
NoneNo IPR availableGENE3D3.30.160.60Classic Zinc Fingercoord: 1..60
e-value: 3.1E-5
score: 25.9
NoneNo IPR availablePANTHERPTHR12801RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATEDcoord: 2..332
NoneNo IPR availablePANTHERPTHR12801:SF122OS02G0709000 PROTEINcoord: 2..332
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 129..299
e-value: 2.6E-29
score: 104.0
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 12..34
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 10..39
score: 11.509996
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 147..288

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh15G003330.1CmaCh15G003330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006364 rRNA processing
molecular_function GO:0008408 3'-5' exonuclease activity
molecular_function GO:0004386 helicase activity
molecular_function GO:0003676 nucleic acid binding