CmaCh15G003070 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh15G003070
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	metalloendoproteinase 5-MMP-like
Location	Cma_Chr15: 1423989 .. 1425692 (-)
RNA-Seq Expression	CmaCh15G003070
Synteny	CmaCh15G003070

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTACGGTTACTTAAGCAAACAAGTACCCAATTCTCATACACACGCATTCGACGAGGCCTTGGAGTCATCTATTAAATCATATCAGAAATTCTTCACGCTTAACGTGAGCGGGGTTTTGGACAAGGAGACGCTAAATCAAATGTCGACCCCTCGATGTGGGGTGACAGATGATTTCAGGTTGGAGGGTGAAAAGGGGAATAATAATAAGAAGAAGAAGAAGAGTATAGAATTTGGTGGTTATTCTTAGATGAAAGTGTCCCATATTGGTTAATTTAGGGAATGTGATATGAACCAAAAAACATCAATCATACAATATACTTCAATGACGGTGTGAAGAAAAAGTTGTCAAAATTATCAAAAGATGTTTCAATGCATGTCACATCGAAGAAGAGTGAAGTTTGATTGTTATAAATTTTGGGTGGGTTACAATTTAGAGTTTTTAGGACTTTTAAGTATGAATTGAAGTATGAATGTTACAACTCTTGGAATGCGTAGTAGTTTCATTTTGAGGCTATATAAAGCCATGTATGTTCTATATGGATGTTGTATTTGTAAGAGACTTGAAAAATATATAGTAAACATTTGTACTTTCTTTAATTGGCTAAGTTTATTTGCAACATCTATGTAGTTTAGGTTGCATGTAGAATCATTCAAGCTCGACATGATCAATCTTGCTTGTGGAGTGATTCGAATCTCAAACAAGTGTTTGAGATATTTGATCAACAAGAACCATTCAAGCTAGACATGATCGATCTTACTTGTGGAGTGATTTGAATCTCAAACAATGTTCTTGCCTTGAGATATTCGATCAACAAGGTAATTCGAATTTTACTCCCCTTGTAATGGTTTCTTATCTTATCAAAATGGTCATGGGTTTATAATCTAAGAATACACTCTCCATTGGTGTGGGGAAGCAGCAAAGCCATGAGAGCTTATGCTCAAAATGGACAATATCATACAATTATGGAGAGTCGTGTTCGTCTAACAGGGTATCAGAGTCATGCTCTAAATTTAGTCGTATCCAATAGATTAACAAATCCTCGAATGTCAAACAAAAGAAATAGGGTATCAGAGCCACAGAGCCATGTCTTAAACTTAGTCGTGCCCAATAGATTGACAAATCCTCGAATGTCGAACAAAGGACTCCAAAAAGAAAAAAAGAAGTCAAGCCTCGATTAAGAGGTTCGAGGGGAGGTATTAGATAAAAGTGTCCCACATTGGTTAATTTAATTTAGGAAATGGTCAAAGTGGACAATATCACACTATTGTAAAGAGATGTACTCATTTATCCACAATTACCGACCCAAGTTTGTGGCTCAAGTAATGCGAGCTTCTAAGACATGGGAGGCCAACACCAATTTCACCTTCTCCCAGTCAGCCAGGGTCCAAACGGCTGACATACAATTAAGCTTCGAGAGAGGGGAGCATGGAGGTGGGCATCCCTTGGATGGATGCGGGGGAGTATTGGCGCATTGTTTTGGGCCAATTGATGGGAGAATCCACTTCGACGCGGATCAGCAGTGGGATTGGAGTGTTCGTATATTGCACAATGACTTGGAGTCTGTGGCATTGCATGAGATTGGGCATGCCCACACCGACGTTCAAGATGCCGTCATGTGGCCTATTATCAACTTTGGATTCACAAAGACCCACCTGCATCACCATGATATTAATGGTATTAGTGCTATATACCCAGATTAA

mRNA sequence

ATGCTACGCAAACAAGTACCCAATTCTCATACACACGCATTCGACGAGGCCTTGGAGTCATCTATTAAATCATATCAGAAATTCTTCACGCTTAACGTGAGCGGGGTTTTGGACAAGGAGACGCTAAATCAAATGTCGACCCCTCGATGTGGGGTGACAGATGATTTCAGGGTCCAAACGGCTGACATACAATTAAGCTTCGAGAGAGGGGAGCATGGAGGTGGGCATCCCTTGGATGGATGCGGGGGAGTATTGGCGCATTGTTTTGGGCCAATTGATGGGAGAATCCACTTCGACGCGGATCAGCAGTGGGATTGGAGTGTTCGTATATTGCACAATGACTTGGAGTCTGTGGCATTGCATGAGATTGGGCATGCCCACACCGACGTTCAAGATGCCGTCATGTGGCCTATTATCAACTTTGGATTCACAAAGACCCACCTGCATCACCATGATATTAATGGTATTAGTGCTATATACCCAGATTAA

Coding sequence (CDS)

ATGCTACGCAAACAAGTACCCAATTCTCATACACACGCATTCGACGAGGCCTTGGAGTCATCTATTAAATCATATCAGAAATTCTTCACGCTTAACGTGAGCGGGGTTTTGGACAAGGAGACGCTAAATCAAATGTCGACCCCTCGATGTGGGGTGACAGATGATTTCAGGGTCCAAACGGCTGACATACAATTAAGCTTCGAGAGAGGGGAGCATGGAGGTGGGCATCCCTTGGATGGATGCGGGGGAGTATTGGCGCATTGTTTTGGGCCAATTGATGGGAGAATCCACTTCGACGCGGATCAGCAGTGGGATTGGAGTGTTCGTATATTGCACAATGACTTGGAGTCTGTGGCATTGCATGAGATTGGGCATGCCCACACCGACGTTCAAGATGCCGTCATGTGGCCTATTATCAACTTTGGATTCACAAAGACCCACCTGCATCACCATGATATTAATGGTATTAGTGCTATATACCCAGATTAA

Protein sequence

MLRKQVPNSHTHAFDEALESSIKSYQKFFTLNVSGVLDKETLNQMSTPRCGVTDDFRVQTADIQLSFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQWDWSVRILHNDLESVALHEIGHAHTDVQDAVMWPIINFGFTKTHLHHHDINGISAIYPD

Homology

[Close]

BLAST of CmaCh15G003070 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.9e-22
Identity = 76/234 (32.48%), Postives = 97/234 (41.45%), Query Frame = 0


Query: 11  THAFDEALESSIKSYQKFFTLNVSGVLDKETLNQMSTPRCG------------------- 70
           T  FD+ L+S+I +YQK F L V+G LD  TL Q+  PRCG                   
Sbjct: 80  TDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRT 139

Query: 71  -----------------------------VTDDFR-----------------------VQ 130
                                        +TD+ +                       + 
Sbjct: 140 TEKYSFFPGKPRWPKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESIL 199

Query: 131 TADIQLSFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQW-----DWSVRILH-- 161
            ADI + F  GEHG G P DG  G LAH   P  G +H D D+ W     + S RIL   
Sbjct: 200 RADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVT 259

[Close]

BLAST of CmaCh15G003070 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 107.1 bits (266), Expect = 1.9e-22
Identity = 73/225 (32.44%), Postives = 96/225 (42.67%), Query Frame = 0


Query: 14  FDEALESSIKSYQKFFTLNVSGVLDKETLNQMSTPRCGVTD---------------DF-- 73
           FD+ L S+IK+YQK + LNV+G  D  TL Q+ TPRCGV D               D+  
Sbjct: 77  FDDTLVSAIKTYQKNYNLNVTGKFDINTLKQIMTPRCGVPDIIINTNKTTSFGMISDYTF 136

Query: 74  ---------------------------------------------------RVQTADIQL 133
                                                                +TA+I++
Sbjct: 137 FKDMPRWQAGTTQLTYAFSPEPRLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKI 196

Query: 134 SFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQWDWSVRILHN------DLESVA 161
            F    HG  +P DG GG+L H F P DGR HFDAD+ W  S  +  +      DLESVA
Sbjct: 197 LFASKNHGDPYPFDGPGGILGHAFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVA 256

[Close]

BLAST of CmaCh15G003070 vs. ExPASy Swiss-Prot
Match: Q8GWW6 (Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 7.6e-19
Identity = 64/225 (28.44%), Postives = 91/225 (40.44%), Query Frame = 0


Query: 15  DEALESSIKSYQKFFTLNVSGVLDKETLNQMSTPRCGVTDDFRVQT-------------- 74
           D + E ++  YQK   L ++G  D +TL+Q+  PRCG  DD   +T              
Sbjct: 71  DVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPRCGFPDDVEPKTAPFHTGKKYVYFPG 130

Query: 75  ---------------------------------------------------------ADI 134
                                                                    ADI
Sbjct: 131 RPRWTRDVPLKLTYAFSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADI 190

Query: 135 QLSFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQW----DWSVRILHNDLESVA 161
           ++ F  G+HG G P DG  GVLAH F P +GR+H D  + W    D     +  DLESVA
Sbjct: 191 KIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVA 250

[Close]

BLAST of CmaCh15G003070 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 2.9e-18
Identity = 47/112 (41.96%), Postives = 63/112 (56.25%), Query Frame = 0


Query: 60  TADIQLSFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQWDWSVRI-------LH 119
           T+DI + F  G+HG G P DG  G LAH F P  G+ H DAD+ W  S  +         
Sbjct: 212 TSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAA 271

Query: 120 NDLESVALHEIGH----AHTDVQDAVMWPIINFGFTKTHLHHHDINGISAIY 161
            DLESVA+HEIGH     H+ V++++M+P I  G  K  L + D+ GI  +Y
Sbjct: 272 VDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

HSP 2 Score: 42.0 bits (97), Expect = 7.7e-03
Identity = 19/44 (43.18%), Postives = 28/44 (63.64%), Query Frame = 0


Query: 11  THAFDEALESSIKSYQKFFTLNVSGVLDKETLNQMSTPRCGVTD 55
           T  FD+ L+++++ YQ  F LNV+G LD  T+  +  PRCG  D
Sbjct: 81  TDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPD 124

[Close]

BLAST of CmaCh15G003070 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 8.4e-18
Identity = 66/245 (26.94%), Postives = 94/245 (38.37%), Query Frame = 0


Query: 11  THAFDEALESSIKSYQKFFTLNVSGVLDKETLNQMSTPRCG------------------- 70
           T  FD+ L+++++ YQ+ F LNV+GVLD+ TL  +  PRCG                   
Sbjct: 84  TDDFDDILKNAVEMYQRNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFE 143

Query: 71  -----------------------------------------VTDDF-------------- 130
                                                    +T++               
Sbjct: 144 VSFAGRGQRFHAVKHYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEV 203

Query: 131 ---------RVQTADIQLSFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQW--- 161
                    R  T+DI + F  GEHG G P DG    LAH F P  G  H D ++ W   
Sbjct: 204 TPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVS 263

[Close]

BLAST of CmaCh15G003070 vs. ExPASy TrEMBL
Match: A0A6J1K202 (metalloendoproteinase 5-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111489405 PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 4.9e-53
Identity = 110/188 (58.51%), Postives = 124/188 (65.96%), Query Frame = 0


Query: 2   LRKQVPNSHTHAFDEALESSIKSYQKFFTLNVSGVLDKETLNQMSTPRCGVTDDF----- 61
           L +   NS T+AFDEALE+SIKSYQKFF LNVSG LDKETL+QM+ PRCG++D+F     
Sbjct: 70  LTQNETNSQTNAFDEALEASIKSYQKFFKLNVSGALDKETLDQMAKPRCGLSDNFTMAGG 129

Query: 62  --------------------RVQTADIQLSFERGEHGGGHPLDGCGGVLAHCFGPIDGRI 121
                               R  T      +   +HG GHP DG GG LAHCFGPIDGR+
Sbjct: 130 KLGGSYYAFFPGRHIWPPEKRHLTYGFIHEYPSKDHGDGHPFDGFGGALAHCFGPIDGRV 189

Query: 122 HFDADQQWDWSVRILHNDLESVALHEIGHA----HTDVQDAVMWPIINFGFTKTHLHHHD 161
           HFDA + WDW VRIL NDLESVALHEI HA    HTDVQDAVMWP +NFG  KT LH  D
Sbjct: 190 HFDAQEVWDWGVRILQNDLESVALHEIRHALGLGHTDVQDAVMWPTMNFGSRKTRLHDDD 249

[Close]

BLAST of CmaCh15G003070 vs. ExPASy TrEMBL
Match: A0A6J1FF28 (metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111445134 PE=4 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 1.9e-49
Identity = 95/110 (86.36%), Postives = 98/110 (89.09%), Query Frame = 0


Query: 57  RVQTADIQLSFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQWDWSVRILHNDLE 116
           RVQTADIQ+SFERGEHG GHPLDG GGVLAHCFGPI GRIHFDADQQWDW VRIL NDLE
Sbjct: 44  RVQTADIQISFERGEHGDGHPLDGYGGVLAHCFGPIAGRIHFDADQQWDWGVRILQNDLE 103

Query: 117 SVALHEIGHA----HTDVQDAVMWPIINFGFTKTHLHHHDINGISAIYPD 163
           SVALHEIGHA    HTDVQDAVMWPIINFGFTKTHLHH DINGI A++ D
Sbjct: 104 SVALHEIGHALGLGHTDVQDAVMWPIINFGFTKTHLHHDDINGIRAMHGD 153

[Close]

BLAST of CmaCh15G003070 vs. ExPASy TrEMBL
Match: A0A5D3BUL7 (Metalloendoproteinase 3-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00140 PE=3 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 4.5e-38
Identity = 102/231 (44.16%), Postives = 116/231 (50.22%), Query Frame = 0


Query: 8   NSHTHAFDEALESSIKSYQKFFTLNVSGVLDKETLNQMSTPRCGVTDDF----------- 67
           N  T AFD+ LES+IKSYQK F LNVSGVLD+ETL QM+ PRCGVTDDF           
Sbjct: 72  NLSTDAFDDDLESAIKSYQKVFKLNVSGVLDRETLQQMARPRCGVTDDFILVAQQDHENN 131

Query: 68  ------------------------------------------------------------ 127
                                                                       
Sbjct: 132 NTNIEIGGSHYMFYPNKLKWPVEKRHLTYGFIHNYPPEHAAAVIRAFDTWEANTKFTFSH 191

Query: 128 --RVQTADIQLSFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQWD-WSVRILHN 161
             RVQTADI LSFERG+HG G P DG GG L H FG IDGR+HFD D+QW+  S    + 
Sbjct: 192 AARVQTADILLSFERGDHGDGKPFDGPGGALGHAFGAIDGRVHFDGDEQWETGSFWQEYF 251

[Close]

BLAST of CmaCh15G003070 vs. ExPASy TrEMBL
Match: A0A1S3C8Z2 (metalloendoproteinase 3-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498174 PE=3 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 2.2e-37
Identity = 101/231 (43.72%), Postives = 115/231 (49.78%), Query Frame = 0


Query: 8   NSHTHAFDEALESSIKSYQKFFTLNVSGVLDKETLNQMSTPRCGVTDDF----------- 67
           N    AFD+ LES+IKSYQK F LNVSGVLD+ETL QM+ PRCGVTDDF           
Sbjct: 72  NLSIDAFDDDLESAIKSYQKVFKLNVSGVLDRETLQQMARPRCGVTDDFILVAQQDHENN 131

Query: 68  ------------------------------------------------------------ 127
                                                                       
Sbjct: 132 NTNIQIGGSHYMFYPNKLKWPVEKRHLTYGFIHNYPPEHAAAVIRAFDTWEANTKFTFSH 191

Query: 128 --RVQTADIQLSFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQWD-WSVRILHN 161
             RVQTADI LSFERG+HG G P DG GG L H FG IDGR+HFD D+QW+  S    + 
Sbjct: 192 AARVQTADILLSFERGDHGDGKPFDGPGGALGHAFGAIDGRVHFDGDEQWETGSFWQEYF 251

[Close]

BLAST of CmaCh15G003070 vs. ExPASy TrEMBL
Match: A0A5D3BRA5 (Metalloendoproteinase 3-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00150 PE=3 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 2.1e-35
Identity = 95/230 (41.30%), Postives = 111/230 (48.26%), Query Frame = 0


Query: 8   NSHTHAFDEALESSIKSYQKFFTLNVSGVLDKETLNQMSTPRCGVTDDF----------- 67
           N  T AFD+ LES+IKSYQKF  + VSGVLD+ETL QMSTPRCGV D F           
Sbjct: 73  NLSTDAFDDDLESAIKSYQKFSKIKVSGVLDRETLQQMSTPRCGVRDTFVSVAQQEHENK 132

Query: 68  ------------------------------------------------------------ 127
                                                                       
Sbjct: 133 STNIEIGGSHYTFYYNNVKWPAEKKHLSYGFIHNFPPQHVPTVLRAFQTWEDNTKFSFFL 192

Query: 128 --RVQTADIQLSFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQWDWSVRILHND 161
             RVQTADI +SFERGEHG   P DG GG+LAH  G +DGR+HFDAD+QW+        D
Sbjct: 193 APRVQTADILVSFERGEHGDDEPFDGPGGILAHSLGAVDGRVHFDADEQWEGGPVEKRYD 252

[Close]

BLAST of CmaCh15G003070 vs. NCBI nr
Match: XP_023551530.1 (metalloendoproteinase 5-MMP-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 223.8 bits (569), Expect = 1.1e-54
Identity = 118/220 (53.64%), Postives = 129/220 (58.64%), Query Frame = 0


Query: 8   NSHTHAFDEALESSIKSYQKFFTLNVSGVLDKETLNQMSTPRCGVTDDFRV--------- 67
           NS T+AFDEALE SIKSYQKFF LNVSG LDKETL+QM+ PRCGV+D+F           
Sbjct: 58  NSQTNAFDEALEESIKSYQKFFKLNVSGALDKETLDQMAKPRCGVSDNFTTAGGKLGGSY 117

Query: 68  ------------------------------------------------------QTADIQ 127
                                                                 QTADI 
Sbjct: 118 YAFFPGRRIWAPEKRHLTYGFIHDYPSKYVPQVMRAFATWEANTHFTFSQAVGGQTADIV 177

Query: 128 LSFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQWDWSVRILHNDLESVALHEIG 161
           LSFER +HG GHP DG GG LAHCFGPIDGR+HFDA + WDW +RIL NDLESVALHEIG
Sbjct: 178 LSFERRDHGDGHPFDGLGGALAHCFGPIDGRVHFDAQEIWDWGIRILQNDLESVALHEIG 237

[Close]

BLAST of CmaCh15G003070 vs. NCBI nr
Match: XP_022993363.1 (metalloendoproteinase 5-MMP-like [Cucurbita maxima])

HSP 1 Score: 217.2 bits (552), Expect = 1.0e-52
Identity = 110/188 (58.51%), Postives = 124/188 (65.96%), Query Frame = 0


Query: 2   LRKQVPNSHTHAFDEALESSIKSYQKFFTLNVSGVLDKETLNQMSTPRCGVTDDF----- 61
           L +   NS T+AFDEALE+SIKSYQKFF LNVSG LDKETL+QM+ PRCG++D+F     
Sbjct: 70  LTQNETNSQTNAFDEALEASIKSYQKFFKLNVSGALDKETLDQMAKPRCGLSDNFTMAGG 129

Query: 62  --------------------RVQTADIQLSFERGEHGGGHPLDGCGGVLAHCFGPIDGRI 121
                               R  T      +   +HG GHP DG GG LAHCFGPIDGR+
Sbjct: 130 KLGGSYYAFFPGRHIWPPEKRHLTYGFIHEYPSKDHGDGHPFDGFGGALAHCFGPIDGRV 189

Query: 122 HFDADQQWDWSVRILHNDLESVALHEIGHA----HTDVQDAVMWPIINFGFTKTHLHHHD 161
           HFDA + WDW VRIL NDLESVALHEI HA    HTDVQDAVMWP +NFG  KT LH  D
Sbjct: 190 HFDAQEVWDWGVRILQNDLESVALHEIRHALGLGHTDVQDAVMWPTMNFGSRKTRLHDDD 249

[Close]

BLAST of CmaCh15G003070 vs. NCBI nr
Match: XP_023551534.1 (metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 205.7 bits (522), Expect = 3.1e-49
Identity = 94/110 (85.45%), Postives = 97/110 (88.18%), Query Frame = 0


Query: 57  RVQTADIQLSFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQWDWSVRILHNDLE 116
           RVQTAD+QLSFERGEHG GHPLDG GG+LAHCFGPIDGRIHFDADQ+WDW VRIL NDLE
Sbjct: 20  RVQTADMQLSFERGEHGDGHPLDGYGGILAHCFGPIDGRIHFDADQEWDWGVRILQNDLE 79

Query: 117 SVALHEIGHA----HTDVQDAVMWPIINFGFTKTHLHHHDINGISAIYPD 163
           SVALHEIGHA    HTDVQ AVMWPIINFGFTKTHLHHH INGI A Y D
Sbjct: 80  SVALHEIGHALGLGHTDVQGAVMWPIINFGFTKTHLHHHHINGIRATYGD 129

[Close]

BLAST of CmaCh15G003070 vs. NCBI nr
Match: XP_022939136.1 (metalloendoproteinase 1-like [Cucurbita moschata])

HSP 1 Score: 205.3 bits (521), Expect = 4.0e-49
Identity = 95/110 (86.36%), Postives = 98/110 (89.09%), Query Frame = 0


Query: 57  RVQTADIQLSFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQWDWSVRILHNDLE 116
           RVQTADIQ+SFERGEHG GHPLDG GGVLAHCFGPI GRIHFDADQQWDW VRIL NDLE
Sbjct: 44  RVQTADIQISFERGEHGDGHPLDGYGGVLAHCFGPIAGRIHFDADQQWDWGVRILQNDLE 103

Query: 117 SVALHEIGHA----HTDVQDAVMWPIINFGFTKTHLHHHDINGISAIYPD 163
           SVALHEIGHA    HTDVQDAVMWPIINFGFTKTHLHH DINGI A++ D
Sbjct: 104 SVALHEIGHALGLGHTDVQDAVMWPIINFGFTKTHLHHDDINGIRAMHGD 153

[Close]

BLAST of CmaCh15G003070 vs. NCBI nr
Match: KAA0037452.1 (metalloendoproteinase 3-MMP-like [Cucumis melo var. makuwa] >TYK01926.1 metalloendoproteinase 3-MMP-like [Cucumis melo var. makuwa])

HSP 1 Score: 167.5 bits (423), Expect = 9.2e-38
Identity = 102/231 (44.16%), Postives = 116/231 (50.22%), Query Frame = 0


Query: 8   NSHTHAFDEALESSIKSYQKFFTLNVSGVLDKETLNQMSTPRCGVTDDF----------- 67
           N  T AFD+ LES+IKSYQK F LNVSGVLD+ETL QM+ PRCGVTDDF           
Sbjct: 72  NLSTDAFDDDLESAIKSYQKVFKLNVSGVLDRETLQQMARPRCGVTDDFILVAQQDHENN 131

Query: 68  ------------------------------------------------------------ 127
                                                                       
Sbjct: 132 NTNIEIGGSHYMFYPNKLKWPVEKRHLTYGFIHNYPPEHAAAVIRAFDTWEANTKFTFSH 191

Query: 128 --RVQTADIQLSFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQWD-WSVRILHN 161
             RVQTADI LSFERG+HG G P DG GG L H FG IDGR+HFD D+QW+  S    + 
Sbjct: 192 AARVQTADILLSFERGDHGDGKPFDGPGGALGHAFGAIDGRVHFDGDEQWETGSFWQEYF 251

[Close]

BLAST of CmaCh15G003070 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 107.1 bits (266), Expect = 1.4e-23
Identity = 76/234 (32.48%), Postives = 97/234 (41.45%), Query Frame = 0


Query: 11  THAFDEALESSIKSYQKFFTLNVSGVLDKETLNQMSTPRCG------------------- 70
           T  FD+ L+S+I +YQK F L V+G LD  TL Q+  PRCG                   
Sbjct: 80  TDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRT 139

Query: 71  -----------------------------VTDDFR-----------------------VQ 130
                                        +TD+ +                       + 
Sbjct: 140 TEKYSFFPGKPRWPKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESIL 199

Query: 131 TADIQLSFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQW-----DWSVRILH-- 161
            ADI + F  GEHG G P DG  G LAH   P  G +H D D+ W     + S RIL   
Sbjct: 200 RADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVT 259

[Close]

BLAST of CmaCh15G003070 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 95.1 bits (235), Expect = 5.4e-20
Identity = 64/225 (28.44%), Postives = 91/225 (40.44%), Query Frame = 0


Query: 15  DEALESSIKSYQKFFTLNVSGVLDKETLNQMSTPRCGVTDDFRVQT-------------- 74
           D + E ++  YQK   L ++G  D +TL+Q+  PRCG  DD   +T              
Sbjct: 71  DVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPRCGFPDDVEPKTAPFHTGKKYVYFPG 130

Query: 75  ---------------------------------------------------------ADI 134
                                                                    ADI
Sbjct: 131 RPRWTRDVPLKLTYAFSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADI 190

Query: 135 QLSFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQW----DWSVRILHNDLESVA 161
           ++ F  G+HG G P DG  GVLAH F P +GR+H D  + W    D     +  DLESVA
Sbjct: 191 KIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVA 250

[Close]

BLAST of CmaCh15G003070 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 93.2 bits (230), Expect = 2.1e-19
Identity = 47/112 (41.96%), Postives = 63/112 (56.25%), Query Frame = 0


Query: 60  TADIQLSFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQWDWSVRI-------LH 119
           T+DI + F  G+HG G P DG  G LAH F P  G+ H DAD+ W  S  +         
Sbjct: 212 TSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAA 271

Query: 120 NDLESVALHEIGH----AHTDVQDAVMWPIINFGFTKTHLHHHDINGISAIY 161
            DLESVA+HEIGH     H+ V++++M+P I  G  K  L + D+ GI  +Y
Sbjct: 272 VDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

HSP 2 Score: 42.0 bits (97), Expect = 5.4e-04
Identity = 19/44 (43.18%), Postives = 28/44 (63.64%), Query Frame = 0


Query: 11  THAFDEALESSIKSYQKFFTLNVSGVLDKETLNQMSTPRCGVTD 55
           T  FD+ L+++++ YQ  F LNV+G LD  T+  +  PRCG  D
Sbjct: 81  TDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPD 124

[Close]

BLAST of CmaCh15G003070 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 91.7 bits (226), Expect = 6.0e-19
Identity = 66/245 (26.94%), Postives = 94/245 (38.37%), Query Frame = 0


Query: 11  THAFDEALESSIKSYQKFFTLNVSGVLDKETLNQMSTPRCG------------------- 70
           T  FD+ L+++++ YQ+ F LNV+GVLD+ TL  +  PRCG                   
Sbjct: 84  TDDFDDILKNAVEMYQRNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFE 143

Query: 71  -----------------------------------------VTDDF-------------- 130
                                                    +T++               
Sbjct: 144 VSFAGRGQRFHAVKHYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEV 203

Query: 131 ---------RVQTADIQLSFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQW--- 161
                    R  T+DI + F  GEHG G P DG    LAH F P  G  H D ++ W   
Sbjct: 204 TPLTFTRVERFSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVS 263

[Close]

BLAST of CmaCh15G003070 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 89.7 bits (221), Expect = 2.3e-18
Identity = 67/225 (29.78%), Postives = 89/225 (39.56%), Query Frame = 0


Query: 14  FDEALESSIKSYQKFFTLNVSGVLDKETLNQMSTPRCGVTD-------DF---------- 73
           FD  LES+I  YQ+   L ++G LD  T+  MS PRCGV+D       DF          
Sbjct: 94  FDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCGVSDTHMTINNDFLHTTAHYTYF 153

Query: 74  ----------------------------------------------------RVQTADIQ 133
                                                                  TAD++
Sbjct: 154 NGKPKWNRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLK 213

Query: 134 LSFERGEHGGGHPLDGCGGVLAHCFGPIDGRIHFDADQQWDWSVRILHN-----DLESVA 161
           + F  G+HG G P DG  G LAH F P +GR+H DA + W     +  +     DLESVA
Sbjct: 214 IGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVA 273

The following BLAST results are available for this feature:

BLAST of CmaCh15G003070 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZUJ5	1.9e-22	32.48	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1	[more]
P29136	1.9e-22	32.44	Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2	[more]
Q8GWW6	7.6e-19	28.44	Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1	[more]
O04529	2.9e-18	41.96	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1	[more]
Q5XF51	8.4e-18	26.94	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1	[more]

BLAST of CmaCh15G003070 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K202	4.9e-53	58.51	metalloendoproteinase 5-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111489405 PE=...	[more]
A0A6J1FF28	1.9e-49	86.36	metalloendoproteinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111445134 PE=4 ...	[more]
A0A5D3BUL7	4.5e-38	44.16	Metalloendoproteinase 3-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3C8Z2	2.2e-37	43.72	metalloendoproteinase 3-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498174 PE=3 SV...	[more]
A0A5D3BRA5	2.1e-35	41.30	Metalloendoproteinase 3-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of CmaCh15G003070 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023551530.1	1.1e-54	53.64	metalloendoproteinase 5-MMP-like [Cucurbita pepo subsp. pepo]	[more]
XP_022993363.1	1.0e-52	58.51	metalloendoproteinase 5-MMP-like [Cucurbita maxima]	[more]
XP_023551534.1	3.1e-49	85.45	metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022939136.1	4.0e-49	86.36	metalloendoproteinase 1-like [Cucurbita moschata]	[more]
KAA0037452.1	9.2e-38	44.16	metalloendoproteinase 3-MMP-like [Cucumis melo var. makuwa] >TYK01926.1 metalloe...	[more]

BLAST of CmaCh15G003070 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G59970.1	1.4e-23	32.48	Matrixin family protein	[more]
AT2G45040.1	5.4e-20	28.44	Matrixin family protein	[more]
AT1G70170.1	2.1e-19	41.96	matrix metalloproteinase	[more]
AT1G24140.1	6.0e-19	26.94	Matrixin family protein	[more]
AT4G16640.1	2.3e-18	29.78	Matrixin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 2..162 e-value: 6.1E-4 score: 9.6
IPR024079	Metallopeptidase, catalytic domain superfamily	GENE3D	3.40.390.10	Collagenase (Catalytic Domain)	coord: 55..162 e-value: 4.6E-29 score: 103.5
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 11..45 e-value: 1.6E-8 score: 34.6
IPR036366	PGBD superfamily	GENE3D	1.10.101.10		coord: 4..54 e-value: 3.6E-16 score: 60.8
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 57..160 e-value: 8.5E-27 score: 93.9
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 10..56
None	No IPR available	PANTHER	PTHR10201:SF272	METALLOENDOPROTEINASE 2-MMP-LIKE	coord: 59..160 coord: 10..56
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 59..160
None	No IPR available	SUPERFAMILY	55486	Metalloproteases ("zincins"), catalytic domain	coord: 7..161

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh15G003070.1	CmaCh15G003070.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity