CmaCh15G002720 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh15G002720
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	ribonuclease DdI-like
Location	Cma_Chr15: 1289223 .. 1290221 (-)
RNA-Seq Expression	CmaCh15G002720
Synteny	CmaCh15G002720

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAACAAGAACACTTTCTTCCTTCTCCTAACCATTTTGATTTTGAATGTTTTGAAAGGGTATGCAATGGCATCAAACGATAGTTCAAATGAGTTAGAAGAGATGAAAGCAGTTCCATTCGATTATTATCAACTCGTGCTACAATGGCAACCAGCAACGTGCAGCAATCTCACCTGCCTGAAACCCTGGTCGTCGAGGTTCAGCATCAACGGCCTCTGGGCTGGCAGCTATTCTGGTAGCATTGGACGGTGCAGTGGCAGCGAATTTCTCCAACAAAACGTATGAATTTATTCACTCTTGTGATTATTGTTAGGAATCACGACTCTTCATAATGATATGATATTTCTTACTTACAAACTAGCACTATAGAGTCTCATAGAAAACCTATAGAGCTATTGAACAGTCTCCCCTTAGTCAAGGCTCACAACTTCTTTGTTCGACATTTGAAGATTCTATTGACATGACTAAGATAAGGGCATAGCTCTGATACCATATTAGAAATCACAACCCTCCATAATGATATGATATTGTCTATTTTAAACATAAGCTCTCATTTGTTTTAGACAACAGATTTCAAGCATAAGAAGAGAGCAAGACGAGGATTGGCCAAGCCTGGTGGTCATAGGCTCAAACCCAGCAGTATGGGGCGAGGAGTGGGATTTACACGGAACCTGCTTCGAGACTCCGACCTTTCAAATCTCAGACTATTTCCGATTAGCTCTGTACCTTTTCTGGAAGAGCGACGTGCTGCAGGCGCTTGAGAAATATGGGATGGAACCCGCGGATGGGCGGCGATATCTAGCACCGGACTTTGAATCTGTCCTCAACGTTAGCTTTGGGAAGCCTGCTCTGCGCTGCAGCCGCAACGTGAAGTATAATCTTCAGTATCAGCTGGCGGAAGTGGGTCTGTGCTTCGACAAGTGTCTTCGCCACATCAACTGCCCTTCCCAGTATTCTGCTGCCAATGGCTGTCCACGAAATCAATTTATCCTCTAG

mRNA sequence

ATGGCAAACAAGAACACTTTCTTCCTTCTCCTAACCATTTTGATTTTGAATGTTTTGAAAGGGTATGCAATGGCATCAAACGATAGTTCAAATGAGTTAGAAGAGATGAAAGCAGTTCCATTCGATTATTATCAACTCGTGCTACAATGGCAACCAGCAACGTGCAGCAATCTCACCTGCCTGAAACCCTGGTCGTCGAGGTTCAGCATCAACGGCCTCTGGGCTGGCAGCTATTCTGGTAGCATTGGACGACAACAGATTTCAAGCATAAGAAGAGAGCAAGACGAGGATTGGCCAAGCCTGGTGGTCATAGGCTCAAACCCAGCAGTATGGGGCGAGGAGTGGGATTTACACGGAACCTGCTTCGAGACTCCGACCTTTCAAATCTCAGACTATTTCCGATTAGCTCTGTACCTTTTCTGGAAGAGCGACGTGCTGCAGGCGCTTGAGAAATATGGGATGGAACCCGCGGATGGGCGGCGATATCTAGCACCGGACTTTGAATCTGTCCTCAACGTTAGCTTTGGGAAGCCTGCTCTGCGCTGCAGCCGCAACGTGAAGTATAATCTTCAGTATCAGCTGGCGGAAGTGGGTCTGTGCTTCGACAAGTGTCTTCGCCACATCAACTGCCCTTCCCAGTATTCTGCTGCCAATGGCTGTCCACGAAATCAATTTATCCTCTAG

Coding sequence (CDS)

ATGGCAAACAAGAACACTTTCTTCCTTCTCCTAACCATTTTGATTTTGAATGTTTTGAAAGGGTATGCAATGGCATCAAACGATAGTTCAAATGAGTTAGAAGAGATGAAAGCAGTTCCATTCGATTATTATCAACTCGTGCTACAATGGCAACCAGCAACGTGCAGCAATCTCACCTGCCTGAAACCCTGGTCGTCGAGGTTCAGCATCAACGGCCTCTGGGCTGGCAGCTATTCTGGTAGCATTGGACGACAACAGATTTCAAGCATAAGAAGAGAGCAAGACGAGGATTGGCCAAGCCTGGTGGTCATAGGCTCAAACCCAGCAGTATGGGGCGAGGAGTGGGATTTACACGGAACCTGCTTCGAGACTCCGACCTTTCAAATCTCAGACTATTTCCGATTAGCTCTGTACCTTTTCTGGAAGAGCGACGTGCTGCAGGCGCTTGAGAAATATGGGATGGAACCCGCGGATGGGCGGCGATATCTAGCACCGGACTTTGAATCTGTCCTCAACGTTAGCTTTGGGAAGCCTGCTCTGCGCTGCAGCCGCAACGTGAAGTATAATCTTCAGTATCAGCTGGCGGAAGTGGGTCTGTGCTTCGACAAGTGTCTTCGCCACATCAACTGCCCTTCCCAGTATTCTGCTGCCAATGGCTGTCCACGAAATCAATTTATCCTCTAG

Protein sequence

MANKNTFFLLLTILILNVLKGYAMASNDSSNELEEMKAVPFDYYQLVLQWQPATCSNLTCLKPWSSRFSINGLWAGSYSGSIGRQQISSIRREQDEDWPSLVVIGSNPAVWGEEWDLHGTCFETPTFQISDYFRLALYLFWKSDVLQALEKYGMEPADGRRYLAPDFESVLNVSFGKPALRCSRNVKYNLQYQLAEVGLCFDKCLRHINCPSQYSAANGCPRNQFIL

Homology

[Close]

BLAST of CmaCh15G002720 vs. ExPASy Swiss-Prot
Match: Q40381 (Ribonuclease S-7 OS=Nicotiana alata OX=4087 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 3.1e-18
Identity = 56/186 (30.11%), Postives = 93/186 (50.00%), Query Frame = 0


Query: 41  FDYYQLVLQWQPATCSNLTCLKPWSSRFSINGLWAGSYSGSI----GRQQISSIRREQDE 100
           F+Y QLVLQW  A C    C K   + F+I+GLW  + S ++     ++   +I  +  +
Sbjct: 24  FEYMQLVLQWPTAFCHTTPC-KRIPNNFTIHGLWPDNVSTTLNYCAAKENFKNIEDDTKK 83

Query: 101 D-----WPSLVVIGS----NPAVWGEEWDLHGTCFETPTFQISDYFRLALYLFWKSDVLQ 160
           D     WP L    +    +   W  E++ HG C  + ++    YF LA+ L  K D+L 
Sbjct: 84  DDLYKRWPDLTTAETYCKQHQNFWRHEYNKHGKCC-SESYNREQYFDLAMALKDKFDLLS 143

Query: 161 ALEKYGMEPADGRRYLAPDFESVL-NVSFGKPALRCSRNVKYNLQYQLAEVGLCFDKCLR 212
           +L  +G+ P  G +Y      S +  ++ G P L C++ +      +L E+G+CFD  ++
Sbjct: 144 SLRNHGIIPGRGMKYTVQKINSTIKKITQGYPNLSCTKGI-----MELVEIGICFDSMVK 202

[Close]

BLAST of CmaCh15G002720 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 5.0e-16
Identity = 68/211 (32.23%), Postives = 97/211 (45.97%), Query Frame = 0


Query: 10  LLTILILNVLKGYAMASNDSSNELEEMKAVPFDYYQLVLQWQPATC-SNLTCLKPWSSR- 69
           L  I +L +L     AS+ S +         FD++  V QW  + C +   C  P S + 
Sbjct: 8   LCLISLLVILPSVFSASSSSED---------FDFFYFVQQWPGSYCDTQKKCCYPNSGKP 67

Query: 70  ---FSINGLWA----GSY------SGSIGRQQISSIRREQDEDWPSLVV-IGSNPAVWGE 129
              F I+GLW     G+Y      S       IS +     + WP+L    GS  A W  
Sbjct: 68  AADFGIHGLWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEH 127

Query: 130 EWDLHGTCFETPTFQISDYFRLALYLFWKSDVLQALEKYGMEPADGRRYLAPDFESVLNV 189
           EW+ HGTC E+   Q  +YF+ AL L  K+++L AL K G+ P DG+ Y        +  
Sbjct: 128 EWEKHGTCSESVIDQ-HEYFQTALNLKQKTNLLGALTKAGINP-DGKSYSLESIRDSIKE 187

Query: 190 SFG-KPALRCSRNVKYNLQYQLAEVGLCFDK 204
           S G  P + C+R+   N   QL +V LC D+
Sbjct: 188 SIGFTPWVECNRDGSGN--SQLYQVYLCVDR 205

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BLAST of CmaCh15G002720 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 1.6e-14
Identity = 62/221 (28.05%), Postives = 95/221 (42.99%), Query Frame = 0


Query: 9   LLLTILILNVLKGYAMASNDSSNELEEMKAVPFDYYQLVLQWQPATC-SNLTCLKPWSSR 68
           LL+ I+++  L    + S D            FD++  V QW  + C +  +C  P + +
Sbjct: 8   LLIKIIVVQCLLVLCVTSQD------------FDFFYFVQQWPASYCDTRRSCCYPTTGK 67

Query: 69  ----FSINGLWAGSYSG----------SIGRQQISSIRREQDEDWPSLVVIGSNP-AVWG 128
               FSI+GLW     G          S+   + S +    +++WPSL    S+    W 
Sbjct: 68  PDEDFSIHGLWPNYKDGKWPQNCDRESSLDESEFSDLISTMEKNWPSLACPSSDGLKFWS 127

Query: 129 EEWDLHGTCFETPTFQISDYFRLALYLFWKSDVLQALEKYGMEPADGRRYLAPDFESVLN 188
            EW  HGTC          YF+ AL    KS++LQ L   G++P +G  Y     +  + 
Sbjct: 128 HEWLKHGTC---SALNQHAYFQTALDFKTKSNLLQNLNNAGIKPRNGDYYGVESIKKAIE 187

Query: 189 VSFG-KPALRCSRNVKYNLQYQLAEVGLCFD-KCLRHINCP 212
              G  P + C  NV     +QL +V LC D    + I+CP
Sbjct: 188 KGVGHTPFIEC--NVDSQGNHQLYQVYLCVDSSASKFIDCP 211

[Close]

BLAST of CmaCh15G002720 vs. ExPASy Swiss-Prot
Match: Q40379 (Ribonuclease S-6 OS=Nicotiana alata OX=4087 PE=1 SV=2)

HSP 1 Score: 79.0 bits (193), Expect = 7.9e-14
Identity = 59/187 (31.55%), Postives = 92/187 (49.20%), Query Frame = 0


Query: 41  FDYYQLVLQWQPATCSNLTCLKPWSSRFSINGLWAGSYSGSI---GRQQISSIRREQDED 100
           F+Y QLVLQW  A C    C K   S F+I+GLW  + S ++   G++   +I  +  E 
Sbjct: 24  FEYMQLVLQWPTAFCHTTPC-KNIPSNFTIHGLWPDNVSTTLNFCGKEDDYNIIMDGPEK 83

Query: 101 ------WPSLVVIGSN----PAVWGEEWDLHGTCFETPTFQISDYFRLALYLFWKSDVLQ 160
                 WP L+   ++       W  E+  HGTC      Q+  YFRLA+ L  K D+L 
Sbjct: 84  NGLYVRWPDLIREKADCMKTQNFWRREYIKHGTCCSEIYNQV-QYFRLAMALKDKFDLLT 143

Query: 161 ALEKYGMEPADGRRYLAPDFESVL-NVSFGKPALRCSRNVKYNLQYQLAEVGLCFDKCLR 213
           +L+ +G+    G +Y      + +  V+ G P L C++        +L  VG+CFD   +
Sbjct: 144 SLKNHGI--IRGYKYTVQKINNTIKTVTKGYPNLSCTKG------QELWFVGICFDSTAK 200

[Close]

BLAST of CmaCh15G002720 vs. ExPASy Swiss-Prot
Match: Q38716 (Ribonuclease S-2 OS=Antirrhinum hispanicum OX=49039 GN=S2 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 57/193 (29.53%), Postives = 87/193 (45.08%), Query Frame = 0


Query: 41  FDYYQLVLQWQPATCSNLTCLKPWS---SRFSINGLWAGSYSGSIG--RQQISSIRREQD 100
           FDY++LVLQW  + CS  T   P +   S+F+I+GLW  + S  +   R   + + +  D
Sbjct: 33  FDYFKLVLQWPNSYCSLKTTHCPRTRLPSQFTIHGLWPDNKSWPLSNCRDTSADVLKITD 92

Query: 101 E--------DWPSLV-----VIGSNPAVWGEEWDLHGTCFETPTFQISDYFRLALYLFWK 160
           +         WP L      V G     W  +W  HG C   P +  +DYF  AL L  +
Sbjct: 93  KGLIQDLAVHWPDLTRRQRKVPGQK--FWVTQWKKHGAC-ALPMYSFNDYFVKALELKKR 152

Query: 161 SDVLQALEKYGMEPADGRRYLAPDFESVLNVSFGKPALRCSRNVKYNLQYQLAEVGLCFD 215
           ++VL  L +  + P D R  ++    ++  V+ G   L+C           L EV +CFD
Sbjct: 153 NNVLDMLSRKSLTPGDQRVDVSDVNGAITKVTGGIAILKCPEG-------YLTEVIICFD 212

[Close]

BLAST of CmaCh15G002720 vs. ExPASy TrEMBL
Match: A0A6J1JWY7 (ribonuclease DdI-like OS=Cucurbita maxima OX=3661 GN=LOC111489590 PE=3 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 5.3e-130
Identity = 226/235 (96.17%), Postives = 226/235 (96.17%), Query Frame = 0


Query: 1   MANKNTFFLLLTILILNVLKGYAMASNDSSNELEEMKAVPFDYYQLVLQWQPATCSNLTC 60
           MANKNTFFLLLTILILNVLKGYAMASNDSSNELEEMKAVPFDYYQLVLQWQPATCSNLTC
Sbjct: 1   MANKNTFFLLLTILILNVLKGYAMASNDSSNELEEMKAVPFDYYQLVLQWQPATCSNLTC 60

Query: 61  LKPWSSRFSINGLWAGSYSGSIGR--------QQISSIRREQDEDWPSLVVIGSNPAVWG 120
           LKPWSSRFSINGLWAGSYSGSIGR        Q ISSIRREQDEDWPSLVVIGSNPAVWG
Sbjct: 61  LKPWSSRFSINGLWAGSYSGSIGRCSGSEFLQQNISSIRREQDEDWPSLVVIGSNPAVWG 120

Query: 121 EEWDLHGTCFETPTFQISDYFRLALYLFWKSDVLQALEKYGMEPADGRRYLAPDFESVLN 180
           EEWDLHGTCFETPTFQISDYFRLALYLFWKSDVLQALEKYGMEPADGRRYLAPDFESVLN
Sbjct: 121 EEWDLHGTCFETPTFQISDYFRLALYLFWKSDVLQALEKYGMEPADGRRYLAPDFESVLN 180

Query: 181 VSFGKPALRCSRNVKYNLQYQLAEVGLCFDKCLRHINCPSQYSAANGCPRNQFIL 228
           VSFGKPALRCSRNVKYNLQYQLAEVGLCFDKCLRHINCPSQYSAANGCPRNQFIL
Sbjct: 181 VSFGKPALRCSRNVKYNLQYQLAEVGLCFDKCLRHINCPSQYSAANGCPRNQFIL 235

[Close]

BLAST of CmaCh15G002720 vs. ExPASy TrEMBL
Match: A0A0A0KS79 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G636520 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 4.4e-76
Identity = 143/228 (62.72%), Postives = 172/228 (75.44%), Query Frame = 0


Query: 10  LLTILILNVLKGYA--MASNDSSNE------LEEMKAVPFDYYQLVLQWQPATCSNLTCL 69
           +L +LI + L G    +A  +S N+      LE MK VPFDYYQLVLQWQPATCSN  CL
Sbjct: 1   MLNLLIGDALNGLEENIAFTESKNDHPSFYGLERMKEVPFDYYQLVLQWQPATCSNAICL 60

Query: 70  KPWSSRFSINGLWAGSYSGSIGR--------QQISSIRREQDEDWPSLVVIGSNPAVWGE 129
           +PWSSRFSINGLWA SYS  IGR        Q I+SIR+E DEDWPSL VI +NPAVW E
Sbjct: 61  RPWSSRFSINGLWAASYSRPIGRCTGNGFLQQNITSIRKELDEDWPSL-VISANPAVWSE 120

Query: 130 EWDLHGTCFETPTFQISDYFRLALYLFWKSDVLQALEKYGMEPADGRRYLAPDFESVLNV 189
            W+L GTCFE+PTFQI+DYFRLALYLFW+SDV +AL++ G+EP +G++Y   D E+ +  
Sbjct: 121 AWNLQGTCFESPTFQINDYFRLALYLFWRSDVQKALQESGIEPINGKQYEKSDIEAAITK 180

Query: 190 SFGKPALRCSRNVKYNLQYQLAEVGLCFDKCLRHINCPSQYSAANGCP 222
           SFGKPALRC+ N+KY LQ QL++V LCFDKCL HI+CPS+YS A GCP
Sbjct: 181 SFGKPALRCNLNLKYLLQSQLSQVFLCFDKCLAHIDCPSKYSPALGCP 227

[Close]

BLAST of CmaCh15G002720 vs. ExPASy TrEMBL
Match: A0A5A7TE29 (Ribonuclease DdI-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold892G00270 PE=3 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 6.0e-73
Identity = 132/201 (65.67%), Postives = 158/201 (78.61%), Query Frame = 0


Query: 29  SSNELEEMKAVPFDYYQLVLQWQPATCSNLTCLKPWSSRFSINGLWAGSYSGSIGR---- 88
           S N+LE MK +PFDYYQLVLQWQPATCS   CL+P+SSRFSINGLWAGSYS  IGR    
Sbjct: 28  SFNDLEGMKEIPFDYYQLVLQWQPATCSAAICLRPFSSRFSINGLWAGSYSRLIGRCIGS 87

Query: 89  ----QQISSIRREQDEDWPSLVVIGSNPAVWGEEWDLHGTCFETPTFQISDYFRLALYLF 148
               Q ++SIR+E D+DWPSL +I SNPAVW E W+LHGTCFETPTFQI+DYFRLALYLF
Sbjct: 88  RFLEQNVTSIRKELDKDWPSL-IISSNPAVWSEAWNLHGTCFETPTFQINDYFRLALYLF 147

Query: 149 WKSDVLQALEKYGMEPADGRRYLAPDFESVLNVSFGKPALRCSRNVKYNLQYQLAEVGLC 208
           W+SDV +AL+ +G+EP +G++Y     E+ +   FGKPALRC+ + KY LQ QLA+V LC
Sbjct: 148 WRSDVQKALQDFGIEPINGKQYEKSYIEAAITEKFGKPALRCNLSGKYFLQSQLAQVVLC 207

Query: 209 FDKCLRHINCPSQYSAANGCP 222
           F+KCL  I+CPSQYS A GCP
Sbjct: 208 FNKCLARIDCPSQYSPALGCP 227

[Close]

BLAST of CmaCh15G002720 vs. ExPASy TrEMBL
Match: A0A6J1DQK2 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.9e-26
Identity = 84/217 (38.71%), Postives = 114/217 (52.53%), Query Frame = 0


Query: 1   MANKNTFFLLLTILILNVLKGYAMASNDSSNELEEMKAVPFDYYQLVLQWQPATCSNLT- 60
           MA ++   L+L++L+L       +  ++  NE        FDYYQ+VLQWQPATCS+ T 
Sbjct: 1   MAKRSEIVLILSMLLL-------IGWSEGVNE--------FDYYQVVLQWQPATCSSSTL 60

Query: 61  ---CLKPWSSRFSINGLWAGSYSGSI---------GRQQISSIRREQDEDWPSLVVIGSN 120
              C++P   RFSI+G+W    SG           G Q  SSIR    +DWPS VV   +
Sbjct: 61  VDECVQPPDERFSIHGVWPSLLSGDAVCSGTPPYDGTQLNSSIRARLHQDWPS-VVHRVD 120

Query: 121 PAVWGEEWDLHGTCFETPTFQISDYFRLALYLFWKSDVLQALEKYGMEPADGRRYLAPDF 180
           P  W  EW LHGTC      Q   YF   L+++   DVL  L  Y + P +G  Y   D 
Sbjct: 121 PPFWSHEWTLHGTCSYAYLDQF-HYFDFGLFVYGNYDVLGILNYYNVYP-NGNLYARSDV 180

Query: 181 ESVLNVSFGK-PALRCSRNVKYNLQYQLAEVGLCFDK 204
           E+++  + GK PALRC+ N  ++   QL E+ LCF K
Sbjct: 181 ENIIYSATGKWPALRCNNNF-WSGDVQLHEISLCFRK 198

[Close]

BLAST of CmaCh15G002720 vs. ExPASy TrEMBL
Match: A0A6J1E0X1 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 1.6e-25
Identity = 77/221 (34.84%), Postives = 118/221 (53.39%), Query Frame = 0


Query: 13  ILILNVLKGYAMASNDSSNELEEMKAVPFDYYQLVLQWQPATCSNLT----CLKPWSSRF 72
           +L+L++    A++S++  NE        FDYYQ+VLQWQPATCS+ T    C++P   R+
Sbjct: 7   LLVLSMF--LAISSSEGVNE--------FDYYQVVLQWQPATCSSATLKTVCVQPPEDRY 66

Query: 73  SINGLWAGSYSGSI---------GRQQISSIRREQDEDWPSLVVIGSNPAVWGEEWDLHG 132
           S++G+W    SG +           + I +I+   D+DWP+ V+ G NP  W  EW  HG
Sbjct: 67  SVHGVWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPN-VITGQNPQFWSYEWTKHG 126

Query: 133 TCFETPTFQISDYFRLALYLFWKSDVLQALEKYGMEPADGRRYLAPDFESVLNVSFGK-P 192
           TC      Q   YF   L+++   D+   L +YG+ P +G++Y     ++ +  + GK P
Sbjct: 127 TCSSAYLNQF-HYFNFGLWVYTNYDLQSILYQYGIYP-NGQQYTKTVVQNAIYSATGKWP 186

Query: 193 ALRCSRNVKYNLQYQLAEVGLCFDKCLR-HINCPSQYSAAN 219
           ALRC+ N +     QL E+ LCF K  +  I+CP      N
Sbjct: 187 ALRCNNNYRTG-NMQLHEISLCFSKNGQILIDCPFNVVTCN 213

[Close]

BLAST of CmaCh15G002720 vs. NCBI nr
Match: XP_022993666.1 (ribonuclease DdI-like [Cucurbita maxima])

HSP 1 Score: 473.4 bits (1217), Expect = 1.1e-129
Identity = 226/235 (96.17%), Postives = 226/235 (96.17%), Query Frame = 0


Query: 1   MANKNTFFLLLTILILNVLKGYAMASNDSSNELEEMKAVPFDYYQLVLQWQPATCSNLTC 60
           MANKNTFFLLLTILILNVLKGYAMASNDSSNELEEMKAVPFDYYQLVLQWQPATCSNLTC
Sbjct: 1   MANKNTFFLLLTILILNVLKGYAMASNDSSNELEEMKAVPFDYYQLVLQWQPATCSNLTC 60

Query: 61  LKPWSSRFSINGLWAGSYSGSIGR--------QQISSIRREQDEDWPSLVVIGSNPAVWG 120
           LKPWSSRFSINGLWAGSYSGSIGR        Q ISSIRREQDEDWPSLVVIGSNPAVWG
Sbjct: 61  LKPWSSRFSINGLWAGSYSGSIGRCSGSEFLQQNISSIRREQDEDWPSLVVIGSNPAVWG 120

Query: 121 EEWDLHGTCFETPTFQISDYFRLALYLFWKSDVLQALEKYGMEPADGRRYLAPDFESVLN 180
           EEWDLHGTCFETPTFQISDYFRLALYLFWKSDVLQALEKYGMEPADGRRYLAPDFESVLN
Sbjct: 121 EEWDLHGTCFETPTFQISDYFRLALYLFWKSDVLQALEKYGMEPADGRRYLAPDFESVLN 180

Query: 181 VSFGKPALRCSRNVKYNLQYQLAEVGLCFDKCLRHINCPSQYSAANGCPRNQFIL 228
           VSFGKPALRCSRNVKYNLQYQLAEVGLCFDKCLRHINCPSQYSAANGCPRNQFIL
Sbjct: 181 VSFGKPALRCSRNVKYNLQYQLAEVGLCFDKCLRHINCPSQYSAANGCPRNQFIL 235

[Close]

BLAST of CmaCh15G002720 vs. NCBI nr
Match: XP_023551239.1 (ribonuclease DdI-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 448.7 bits (1153), Expect = 2.9e-122
Identity = 216/241 (89.63%), Postives = 220/241 (91.29%), Query Frame = 0


Query: 1   MANKNTFFLLLTILILNVLKGYAM------ASNDSSNELEEMKAVPFDYYQLVLQWQPAT 60
           MAN+NTFFLLLTILILNVLKGYA+       SNDS NELEEMKAVPFDYYQLVLQWQPAT
Sbjct: 1   MANENTFFLLLTILILNVLKGYALNGIASVESNDSLNELEEMKAVPFDYYQLVLQWQPAT 60

Query: 61  CSNLTCLKPWSSRFSINGLWAGSYSGSIGR--------QQISSIRREQDEDWPSLVVIGS 120
           CSNLTCLKPWSSRFSINGLWAGSYSGSIGR        Q ISSIRRE DEDWPSLVVIGS
Sbjct: 61  CSNLTCLKPWSSRFSINGLWAGSYSGSIGRCNGSEFLQQNISSIRRELDEDWPSLVVIGS 120

Query: 121 NPAVWGEEWDLHGTCFETPTFQISDYFRLALYLFWKSDVLQALEKYGMEPADGRRYLAPD 180
           NPAVWG+EWDLHGTC ETPTFQISDYFRLALYLFW+SDVLQALEKYGMEP DGRRYLAPD
Sbjct: 121 NPAVWGDEWDLHGTCLETPTFQISDYFRLALYLFWRSDVLQALEKYGMEPVDGRRYLAPD 180

Query: 181 FESVLNVSFGKPALRCSRNVKYNLQYQLAEVGLCFDKCLRHINCPSQYSAANGCPRNQFI 228
           FESVLNVSFGKPALRCSRNVKYNLQYQLAEV LCFDKCLRHINCPSQYSAANGCPRNQFI
Sbjct: 181 FESVLNVSFGKPALRCSRNVKYNLQYQLAEVALCFDKCLRHINCPSQYSAANGCPRNQFI 240

[Close]

BLAST of CmaCh15G002720 vs. NCBI nr
Match: KAG6578584.1 (Ribonuclease DdI, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 439.5 bits (1129), Expect = 1.8e-119
Identity = 213/241 (88.38%), Postives = 216/241 (89.63%), Query Frame = 0


Query: 1   MANKNTFFLLLTILILNVLKGYA------MASNDSSNELEEMKAVPFDYYQLVLQWQPAT 60
           MANKNTFFLLLTILILNVLKGY       M S DSSNELEEMKAV FDYYQLVLQWQPAT
Sbjct: 1   MANKNTFFLLLTILILNVLKGYGLDDIAFMESYDSSNELEEMKAVLFDYYQLVLQWQPAT 60

Query: 61  CSNLTCLKPWSSRFSINGLWAGSYSGSIG--------RQQISSIRREQDEDWPSLVVIGS 120
           CSNLTCLKPWSSRFSINGLWAGSYS SIG        +Q ISSIRRE DEDWP LVVIGS
Sbjct: 61  CSNLTCLKPWSSRFSINGLWAGSYSRSIGQCSGSEFLQQNISSIRRELDEDWPRLVVIGS 120

Query: 121 NPAVWGEEWDLHGTCFETPTFQISDYFRLALYLFWKSDVLQALEKYGMEPADGRRYLAPD 180
           NPAVWG+EWDLHGTCFETPTFQISDYFRL LYLFW+SDVLQALEKYGMEP DGRRYLAPD
Sbjct: 121 NPAVWGDEWDLHGTCFETPTFQISDYFRLGLYLFWRSDVLQALEKYGMEPVDGRRYLAPD 180

Query: 181 FESVLNVSFGKPALRCSRNVKYNLQYQLAEVGLCFDKCLRHINCPSQYSAANGCPRNQFI 228
           FESVLNVSFGKPALRCSRNVKYNLQYQLAEV LCFDKCLRHINCPSQYSAANGCPRNQFI
Sbjct: 181 FESVLNVSFGKPALRCSRNVKYNLQYQLAEVALCFDKCLRHINCPSQYSAANGCPRNQFI 240

[Close]

BLAST of CmaCh15G002720 vs. NCBI nr
Match: KAG7016138.1 (Ribonuclease DdI, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 380.9 bits (977), Expect = 7.4e-102
Identity = 187/233 (80.26%), Postives = 190/233 (81.55%), Query Frame = 0


Query: 1   MANKNTFFLLLTILILNVLKGYA------MASNDSSNELEEMKAVPFDYYQLVLQWQPAT 60
           MANKNTFFLLLTILILNVLKGY       M S DSSNELEEMKAV FDYYQLVLQWQPAT
Sbjct: 1   MANKNTFFLLLTILILNVLKGYGLDDIAFMESYDSSNELEEMKAVLFDYYQLVLQWQPAT 60

Query: 61  CSNLTCLKPWSSRFSINGLWAGSYSGSIGRQQISSIRREQDEDWPSLVVIGSNPAVWGEE 120
           CSNLTCLKPWSSRFSINGLWAGSYS                              +WG+E
Sbjct: 61  CSNLTCLKPWSSRFSINGLWAGSYS------------------------------LWGDE 120

Query: 121 WDLHGTCFETPTFQISDYFRLALYLFWKSDVLQALEKYGMEPADGRRYLAPDFESVLNVS 180
           WDLHGTCFETPTFQISDYFRL LYLFW+SDVLQALEKYGMEP DGRRYLAPDFESVLNVS
Sbjct: 121 WDLHGTCFETPTFQISDYFRLGLYLFWRSDVLQALEKYGMEPVDGRRYLAPDFESVLNVS 180

Query: 181 FGKPALRCSRNVKYNLQYQLAEVGLCFDKCLRHINCPSQYSAANGCPRNQFIL 228
           FGKPALRCSRNVKYNLQYQLAEV LCFDKCLRHINCPSQYSAANGCPRNQFIL
Sbjct: 181 FGKPALRCSRNVKYNLQYQLAEVALCFDKCLRHINCPSQYSAANGCPRNQFIL 203

[Close]

BLAST of CmaCh15G002720 vs. NCBI nr
Match: XP_038889664.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 315.5 bits (807), Expect = 3.8e-82
Identity = 161/256 (62.89%), Postives = 185/256 (72.27%), Query Frame = 0


Query: 1   MANKNTFFLLLTILILNVLKG---------------------YAMASNDSSN------EL 60
           M+ K + FLLL IL+LN+L G                     Y +A  +S N      +L
Sbjct: 3   MSKKKSLFLLLCILLLNLLIGDALDELEDDDIKNEDAFNELKYELAFMESKNNDHSFYDL 62

Query: 61  EEMKAVPFDYYQLVLQWQPATCSNLTCLKPWSSRFSINGLWAGSYSGSIGR--------Q 120
           EEMKAVPFDYYQLVLQWQPATCSN+ CL+P SSRFSI+GLWAGSYS  IGR        Q
Sbjct: 63  EEMKAVPFDYYQLVLQWQPATCSNVICLRPTSSRFSIDGLWAGSYSRPIGRCSGSKFLQQ 122

Query: 121 QISSIRREQDEDWPSLVVIGSNPAVWGEEWDLHGTCFETPTFQISDYFRLALYLFWKSDV 180
            I+SIR+E DEDWPSL +I SNPAVW E W+L GTCFETPTFQI DYFRLALYLFW+SDV
Sbjct: 123 NITSIRKELDEDWPSL-IISSNPAVWSEAWNLRGTCFETPTFQIVDYFRLALYLFWRSDV 182

Query: 181 LQALEKYGMEPADGRRYLAPDFESVLNVSFGKPALRCSRNVKYNLQYQLAEVGLCFDKCL 222
            QAL+K G+EP +G+RYL  D E+ L  SFGKPALRC+ N+KY LQ QLAEV LCFDKCL
Sbjct: 183 QQALQKSGLEPINGKRYLKSDIETALTKSFGKPALRCTLNLKYLLQSQLAEVVLCFDKCL 242

[Close]

BLAST of CmaCh15G002720 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 86.3 bits (212), Expect = 3.5e-17
Identity = 68/211 (32.23%), Postives = 97/211 (45.97%), Query Frame = 0


Query: 10  LLTILILNVLKGYAMASNDSSNELEEMKAVPFDYYQLVLQWQPATC-SNLTCLKPWSSR- 69
           L  I +L +L     AS+ S +         FD++  V QW  + C +   C  P S + 
Sbjct: 8   LCLISLLVILPSVFSASSSSED---------FDFFYFVQQWPGSYCDTQKKCCYPNSGKP 67

Query: 70  ---FSINGLWA----GSY------SGSIGRQQISSIRREQDEDWPSLVV-IGSNPAVWGE 129
              F I+GLW     G+Y      S       IS +     + WP+L    GS  A W  
Sbjct: 68  AADFGIHGLWPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEH 127

Query: 130 EWDLHGTCFETPTFQISDYFRLALYLFWKSDVLQALEKYGMEPADGRRYLAPDFESVLNV 189
           EW+ HGTC E+   Q  +YF+ AL L  K+++L AL K G+ P DG+ Y        +  
Sbjct: 128 EWEKHGTCSESVIDQ-HEYFQTALNLKQKTNLLGALTKAGINP-DGKSYSLESIRDSIKE 187

Query: 190 SFG-KPALRCSRNVKYNLQYQLAEVGLCFDK 204
           S G  P + C+R+   N   QL +V LC D+
Sbjct: 188 SIGFTPWVECNRDGSGN--SQLYQVYLCVDR 205

[Close]

BLAST of CmaCh15G002720 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 84.7 bits (208), Expect = 1.0e-16
Identity = 58/188 (30.85%), Postives = 88/188 (46.81%), Query Frame = 0


Query: 41  FDYYQLVLQWQPATC-SNLTCLKPWSSR----FSINGLWAGSYSGS----------IGRQ 100
           FD++  VLQW  A C ++  C  P S +    F I+GLW     GS            R 
Sbjct: 28  FDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLWPNYNGGSWPSNCDPDSQFDRS 87

Query: 101 QISSIRREQDEDWPSLVVIGSNP-AVWGEEWDLHGTCFETPTFQISDYFRLALYLFWKSD 160
           QIS +     ++WP+L    +     W  EW+ HGTC E+   Q  +YF  AL L  K++
Sbjct: 88  QISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQ-HEYFENALKLKQKAN 147

Query: 161 VLQALEKYGMEPADGRRYLAPDFESVLNVSFGKPALRCSRNVKYNLQYQLAEVGLCFDKC 212
           +LQ L+  G+ P DG   L     ++ +     P + C+++ + N   QL ++ +C D  
Sbjct: 148 LLQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGIECNKDPERNA--QLHQIYICVDTS 207

The following BLAST results are available for this feature:

BLAST of CmaCh15G002720 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q40381	3.1e-18	30.11	Ribonuclease S-7 OS=Nicotiana alata OX=4087 PE=1 SV=1	[more]
P42813	5.0e-16	32.23	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P80196	1.6e-14	28.05	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]
Q40379	7.9e-14	31.55	Ribonuclease S-6 OS=Nicotiana alata OX=4087 PE=1 SV=2	[more]
Q38716	1.1e-12	29.53	Ribonuclease S-2 OS=Antirrhinum hispanicum OX=49039 GN=S2 PE=2 SV=1	[more]

BLAST of CmaCh15G002720 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JWY7	5.3e-130	96.17	ribonuclease DdI-like OS=Cucurbita maxima OX=3661 GN=LOC111489590 PE=3 SV=1	[more]
A0A0A0KS79	4.4e-76	62.72	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G636520 PE=3 SV=1	[more]
A0A5A7TE29	6.0e-73	65.67	Ribonuclease DdI-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold89...	[more]
A0A6J1DQK2	1.9e-26	38.71	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1	[more]
A0A6J1E0X1	1.6e-25	34.84	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1	[more]

BLAST of CmaCh15G002720 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022993666.1	1.1e-129	96.17	ribonuclease DdI-like [Cucurbita maxima]	[more]
XP_023551239.1	2.9e-122	89.63	ribonuclease DdI-like [Cucurbita pepo subsp. pepo]	[more]
KAG6578584.1	1.8e-119	88.38	Ribonuclease DdI, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7016138.1	7.4e-102	80.26	Ribonuclease DdI, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_038889664.1	3.8e-82	62.89	ribonuclease MC-like [Benincasa hispida]	[more]

BLAST of CmaCh15G002720 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	3.5e-17	32.23	ribonuclease 1	[more]
AT1G14220.1	1.0e-16	30.85	Ribonuclease T2 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 41..210 e-value: 2.4E-25 score: 89.8
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 12..223
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 35..226 e-value: 2.8E-36 score: 127.4
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 39..223
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 12..223

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh15G002720.1	CmaCh15G002720.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding