CmaCh15G000850 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh15G000850 vs. ExPASy Swiss-Prot
Match: Q8S9J1 (Protoporphyrinogen oxidase 2, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PPOX2 PE=1 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 5.8e-187
Identity = 339/502 (67.53%), Postives = 395/502 (78.69%), Query Frame = 0

Query: 1   MASPAKQD---QRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS--- 60
           MAS A  D   +  S KRVAVVGAGVSGLAAAYKLK+ G NVTV EADGR GGKLRS   
Sbjct: 1   MASGAVADHQIEAVSGKRVAVVGAGVSGLAAAYKLKSRGLNVTVFEADGRVGGKLRSVMQ 60

Query: 61  ------------TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLI 120
                       TE+EPEV  L DDLGLREKQQFPISQ KRYIVRNG+PV++PT+PI L+
Sbjct: 61  NGLIWDEGANTMTEAEPEVGSLLDDLGLREKQQFPISQKKRYIVRNGVPVMLPTNPIELV 120

Query: 121 KSNILSTKSKFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAG 180
            S++LST+SKFQI+LEPFLWKK  +SKVS+   +ESV  FFQRHFG+EVVDYLIDP V G
Sbjct: 121 TSSVLSTQSKFQILLEPFLWKK-KSSKVSDASAEESVSEFFQRHFGQEVVDYLIDPFVGG 180

Query: 181 TSAGDPDSLSMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGS 240
           TSA DPDSLSM HSFP+LWN+EK FGSI+ G + +K + K         + G +K +RGS
Sbjct: 181 TSAADPDSLSMKHSFPDLWNVEKSFGSIIVGAIRTKFAAKGGKSRDTKSSPGTKKGSRGS 240

Query: 241 FSFQNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIAD 300
           FSF+ GMQ L DTL K L  D + L S+VLSLSYN+G S  +NWSL+  S   ++     
Sbjct: 241 FSFKGGMQILPDTLCKSLSHDEINLDSKVLSLSYNSG-SRQENWSLSCVSHNETQRQNPH 300

Query: 301 ADAVIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVL 360
            DAVIMTAP+CNV+EMK MKGG PF LNFLPE+ YMPLSV+ITTF KE VKRPLEGFGVL
Sbjct: 301 YDAVIMTAPLCNVKEMKVMKGGQPFQLNFLPEINYMPLSVLITTFTKEKVKRPLEGFGVL 360

Query: 361 VPSLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSD 420
           +PS EQ++G +TLGTLFSSMMFP+R+ +D +LYTTFIGGSRN+ELAK+STDELKQ+VTSD
Sbjct: 361 IPSKEQKHGFKTLGTLFSSMMFPDRSPSDVHLYTTFIGGSRNQELAKASTDELKQVVTSD 420

Query: 421 LRQLLGVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVG 480
           L++LLGVEGEP  VNH+YW KAFPLY  +YDSVM+AI+ ME  LPGFFYAGNHR GLSVG
Sbjct: 421 LQRLLGVEGEPVSVNHYYWRKAFPLYDSSYDSVMEAIDKMENDLPGFFYAGNHRGGLSVG 480

Query: 481 KSIASGCKAADLVISYLESSTD 485
           KSIASGCKAADLVISYLES ++
Sbjct: 481 KSIASGCKAADLVISYLESCSN 500

BLAST of CmaCh15G000850 vs. ExPASy Swiss-Prot
Match: Q94IG7 (Protoporphyrinogen oxidase 2 OS=Spinacia oleracea OX=3562 GN=POX2 PE=1 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 5.8e-187
Identity = 337/491 (68.64%), Postives = 399/491 (81.26%), Query Frame = 0

Query: 8   DQRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS------------- 67
           +Q  S KRVAVVGAGVSGLAAAYKLK++G NVT+ EAD RAGGKL++             
Sbjct: 37  NQPISAKRVAVVGAGVSGLAAAYKLKSNGLNVTLFEADSRAGGKLKTVVKDGLIWDEGAN 96

Query: 68  --TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSNILSTKSK 127
             TES+ EV  LFDDLG+REK Q PISQ+KRYI R+GLPVL+P++P+ L+KSNILS KSK
Sbjct: 97  TMTESDEEVTSLFDDLGIREKLQLPISQNKRYIARDGLPVLLPSNPVALLKSNILSAKSK 156

Query: 128 FQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSAGDPDSLS 187
            QI+LEPFLWKK+N +KVS+++  ESV  FF+RHFGKE VDYLIDP VAGTS GDP SLS
Sbjct: 157 LQIMLEPFLWKKHNGAKVSDENAQESVAEFFERHFGKEFVDYLIDPFVAGTSGGDPQSLS 216

Query: 188 MSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGSFSFQNGMQTL 247
           M H+FPELWNIE RFGS+++G + SKLS+KKE  G +  ++ K+   RGSFSFQ GMQTL
Sbjct: 217 MRHAFPELWNIENRFGSVISGFIQSKLSSKKEKGGEKQ-SSNKKPRVRGSFSFQGGMQTL 276

Query: 248 TDTLSKELGEDVLKLRSEVLSLSY-NAGKSASQNWSLTYSSDRNSKNLIADADAVIMTAP 307
            DT+ KE GED LKL+SEVLSLSY + G   S+NWS+  SS  NS       DAV++TAP
Sbjct: 277 VDTICKEFGEDELKLQSEVLSLSYSHNGSLTSENWSV--SSMSNSTIQDQPYDAVVVTAP 336

Query: 308 VCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNG 367
           + NV+E+K MK   PF L+F+PEV+ +PLSV+ITTF+K +VKRPLEGFGVLVPS EQ NG
Sbjct: 337 INNVKELKIMKVENPFSLDFIPEVSCLPLSVIITTFKKTNVKRPLEGFGVLVPSNEQHNG 396

Query: 368 LRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQLLGVEG 427
           L+TLGTLFSSMMFP+RA +D YLYTTF+GGSRNRELAK+STDELKQIV+SDL+QLLG EG
Sbjct: 397 LKTLGTLFSSMMFPDRAPSDVYLYTTFVGGSRNRELAKASTDELKQIVSSDLQQLLGTEG 456

Query: 428 EPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKA 483
           EPTFVNHFYWSKAFPLYGRNYDSV++AIE ME+ LPG FYAGNH+ GLSVGKSIASG KA
Sbjct: 457 EPTFVNHFYWSKAFPLYGRNYDSVLRAIEKMERDLPGLFYAGNHKGGLSVGKSIASGYKA 516

BLAST of CmaCh15G000850 vs. ExPASy Swiss-Prot
Match: O24164 (Protoporphyrinogen oxidase, mitochondrial OS=Nicotiana tabacum OX=4097 GN=PPXII PE=1 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 6.7e-183
Identity = 334/501 (66.67%), Postives = 391/501 (78.04%), Query Frame = 0

Query: 1   MASPAKQDQRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS------ 60
           MA  A +D+ +S KRVAV+GAGVSGLAAAYKLK HG NVTV EA+G+AGGKLRS      
Sbjct: 1   MAPSAGEDKHSSAKRVAVIGAGVSGLAAAYKLKIHGLNVTVFEAEGKAGGKLRSVSQDGL 60

Query: 61  ---------TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSN 120
                    TESE +V  L D LGLREKQQFP+SQ+KRYI RNG PVL+P++PI LIKSN
Sbjct: 61  IWDEGANTMTESEGDVTFLIDSLGLREKQQFPLSQNKRYIARNGTPVLLPSNPIDLIKSN 120

Query: 121 ILSTKSKFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSA 180
            LST SK Q++LEP LWK    S+VS  D+ ESV GFFQRHFGKEVVDYLIDP VAGT  
Sbjct: 121 FLSTGSKLQMLLEPILWKNKKLSQVS--DSHESVSGFFQRHFGKEVVDYLIDPFVAGTCG 180

Query: 181 GDPDSLSMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGSFSF 240
           GDPDSLSM HSFPELWN+EKRFGS++ G + SKLS K E +     T+  +K  RGSFSF
Sbjct: 181 GDPDSLSMHHSFPELWNLEKRFGSVILGAIRSKLSPKNEKKQGPPKTSANKKRQRGSFSF 240

Query: 241 QNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGK-SASQNWSLTYSSDRNSKNLIADAD 300
             GMQTLTD + K+L ED L+L S VL LS +  + SA  +WS+  +S    ++     D
Sbjct: 241 LGGMQTLTDAICKDLREDELRLNSRVLELSCSCTEDSAIDSWSIISASPHKRQSEEESFD 300

Query: 301 AVIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVLVP 360
           AVIMTAP+C+V+ MK  K G PF LNF+PEV Y+PLSV+ITTF++E+VK PLEGFGVLVP
Sbjct: 301 AVIMTAPLCDVKSMKIAKRGNPFLLNFIPEVDYVPLSVVITTFKRENVKYPLEGFGVLVP 360

Query: 361 SLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLR 420
           S EQQ+GL+TLGTLFSSMMFP+RA N+ YLYTTF+GGSRNRELAK+S  ELK+IVTSDL+
Sbjct: 361 SKEQQHGLKTLGTLFSSMMFPDRAPNNVYLYTTFVGGSRNRELAKASRTELKEIVTSDLK 420

Query: 421 QLLGVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVGKS 480
           QLLG EGEPT+VNH YWSKAFPLYG NYDSV+ AI+ MEK+LPG FYAGNHR GLSVGK+
Sbjct: 421 QLLGAEGEPTYVNHLYWSKAFPLYGHNYDSVLDAIDKMEKNLPGLFYAGNHRGGLSVGKA 480

Query: 481 IASGCKAADLVISYLES-STD 485
           ++SGC AADLVISYLES STD
Sbjct: 481 LSSGCNAADLVISYLESVSTD 499

BLAST of CmaCh15G000850 vs. ExPASy Swiss-Prot
Match: O24163 (Protoporphyrinogen oxidase, chloroplastic OS=Nicotiana tabacum OX=4097 GN=PPXI PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 5.3e-39
Identity = 144/500 (28.80%), Postives = 236/500 (47.20%), Query Frame = 0

Query: 18  VVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRSTE---------------SEPEVQ 77
           +VGAG+SGL  A  + A+  N+ V EA  RAGG + + E               S+P + 
Sbjct: 76  IVGAGISGLCIAQVMSANYPNLMVTEARDRAGGNITTVERDGYLWEEGPNSFQPSDP-ML 135

Query: 78  CLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSNILSTKSKFQ-----IIL 137
            +  D GL++        + R+++  G    VP+    L   +++S   K +     I L
Sbjct: 136 TMAVDCGLKDDLVLGDPNAPRFVLWKGKLRPVPSKLTDLAFFDLMSIPGKLRAGFGAIGL 195

Query: 138 EPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSAGDPDSLSMSHSF 197
            P           S    +ESV  F +R+ G EV + LI+P  +G  AGDP  LSM  +F
Sbjct: 196 RP-----------SPPGHEESVEQFVRRNLGGEVFERLIEPFCSGVYAGDPSKLSMKAAF 255

Query: 198 PELWNIEKRFGSILAG---LVLSKLSTKKENRGVRNGTTGKRKPTRGSFSFQNGMQTLTD 257
            ++W +E+  GSI+ G    +  + ST K  R  R      +  T G  SF+ G++ L D
Sbjct: 256 GKVWKLEETGGSIIGGTFKAIKERSSTPKAPRDPR--LPKPKGQTVG--SFRKGLRMLPD 315

Query: 258 TLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADAVIMTAPVCN 317
            +S  LG   LKL  ++ S++    KS    + LTY +     +L   + +++MT P   
Sbjct: 316 AISARLGSK-LKLSWKLSSIT----KSEKGGYHLTYETPEGVVSL--QSRSIVMTVP--- 375

Query: 318 VREMKFMKGGIPFPLNF-----LPEVAYMPLSVMITTFRKESVK------RPLEGFGVLV 377
                ++   I  PL+      L    Y P+  +  T+ +E+++        L+GFG L 
Sbjct: 376 ----SYVASNILRPLSVAAADALSNFYYPPVGAVTITYPQEAIRDERLVDGELKGFGQLH 435

Query: 378 PSLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDL 437
           P   +  G+ TLGT++SS +FPNRA     L   +IGG++N E+   +  +L ++V  DL
Sbjct: 436 P---RTQGVETLGTIYSSSLFPNRAPKGRVLLLNYIGGAKNPEILSKTESQLVEVVDRDL 495

Query: 438 RQLL--GVEGEPTFVNHFYWSKAFPLY-GRNYDSVMKAIETM-EKSLPGFFYAGNHRDGL 480
           R++L      +P  V    W +A P +   + D++  A   M +  L G F  GN+  G+
Sbjct: 496 RKMLIKPKAQDPLVVGVRVWPQAIPQFLVGHLDTLSTAKAAMNDNGLEGLFLGGNYVSGV 542

BLAST of CmaCh15G000850 vs. ExPASy Swiss-Prot
Match: Q9AR38 (Protoporphyrinogen oxidase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=PPOX1 PE=2 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 3.8e-37
Identity = 143/513 (27.88%), Postives = 236/513 (46.00%), Query Frame = 0

Query: 3   SPAKQDQRTSRKRVAVVGAGVSGLAAAYKLKA-HGF-NVTVLEADGRAGGKLRSTESEPE 62
           +PA    R S   V VVG G+SGL  A  L   HG  +V V EA  R GG + + E   E
Sbjct: 46  APAAPGARVSADCV-VVGGGISGLCTAQALATKHGVGDVLVTEARARPGGNITTAERAGE 105

Query: 63  ----------------VQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKS 122
                           V  +  D GL++   F    + R+++  G    VP+ P  L   
Sbjct: 106 GYLWEEGPNSFQPSDPVLTMAVDSGLKDDLVFGDPNAPRFVLWEGKLRPVPSKPGDLPFF 165

Query: 123 NILSTKSKFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTS 182
           +++S   K +  L           +      +ESV  F +R+ G EV + LI+P  +G  
Sbjct: 166 DLMSIPGKLRAGLGAL------GVRAPPPGREESVEDFVRRNLGAEVFERLIEPFCSGVY 225

Query: 183 AGDPDSLSMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPT---RG 242
           AGDP  LSM  +F ++W +E   GSI+ G + +     K  +  R+     R PT   + 
Sbjct: 226 AGDPSKLSMKAAFGKVWRLEDTGGSIIGGTIKTIQERGKNPKPPRD----PRLPTPKGQT 285

Query: 243 SFSFQNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIA 302
             SF+ G+  L D ++  LG  V KL  ++ S++    KS ++ ++L Y +     ++  
Sbjct: 286 VASFRKGLTMLPDAITSRLGSKV-KLSWKLTSIT----KSDNKGYALVYETPEGVVSV-- 345

Query: 303 DADAVIMTAPVCNVREMKFMKGGIPFPL-----NFLPEVAYMPLSVMITTFRKESVKR-- 362
            A  V+MT P        ++   I  PL     + L    Y P++ +  ++ KE++++  
Sbjct: 346 QAKTVVMTIP-------SYVASDILRPLSSDAADALSIFYYPPVAAVTVSYPKEAIRKEC 405

Query: 363 ----PLEGFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKS 422
                L+GFG L P   +  G+ TLGT++SS +FPNRA     L   +IGGS N  +   
Sbjct: 406 LIDGELQGFGQLHP---RSQGVETLGTIYSSSLFPNRAPAGRVLLLNYIGGSTNTGIVSK 465

Query: 423 STDELKQIVTSDLRQLL--GVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIETM--EKSL 480
           +  EL + V  DLR++L      +P  +    W +A P +   +   ++A ++   +   
Sbjct: 466 TESELVEAVDRDLRKMLINPKAVDPLVLGVRVWPQAIPQFLIGHLDHLEAAKSALGKGGY 525

BLAST of CmaCh15G000850 vs. ExPASy TrEMBL
Match: A0A6J1JQC4 (Protoporphyrinogen oxidase OS=Cucurbita maxima OX=3661 GN=LOC111488903 PE=3 SV=1)

HSP 1 Score: 967.2 bits (2499), Expect = 2.6e-278
Identity = 495/510 (97.06%), Postives = 495/510 (97.06%), Query Frame = 0

Query: 1   MASPAKQDQRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS------ 60
           MASPAKQDQRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS      
Sbjct: 1   MASPAKQDQRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRSVSYNGL 60

Query: 61  ---------TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSN 120
                    TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSN
Sbjct: 61  IWDEGANTMTESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSN 120

Query: 121 ILSTKSKFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSA 180
           ILSTKSKFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSA
Sbjct: 121 ILSTKSKFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSA 180

Query: 181 GDPDSLSMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGSFSF 240
           GDPDSLSMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGSFSF
Sbjct: 181 GDPDSLSMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGSFSF 240

Query: 241 QNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADA 300
           QNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADA
Sbjct: 241 QNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADA 300

Query: 301 VIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVLVPS 360
           VIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVLVPS
Sbjct: 301 VIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVLVPS 360

Query: 361 LEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQ 420
           LEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQ
Sbjct: 361 LEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQ 420

Query: 421 LLGVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVGKSI 480
           LLGVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVGKSI
Sbjct: 421 LLGVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVGKSI 480

Query: 481 ASGCKAADLVISYLESSTDQPCAEQQELKK 496
           ASGCKAADLVISYLESSTDQPCAEQQELKK
Sbjct: 481 ASGCKAADLVISYLESSTDQPCAEQQELKK 510

BLAST of CmaCh15G000850 vs. ExPASy TrEMBL
Match: A0A6J1JY02 (Protoporphyrinogen oxidase OS=Cucurbita maxima OX=3661 GN=LOC111488903 PE=3 SV=1)

HSP 1 Score: 947.6 bits (2448), Expect = 2.1e-272
Identity = 484/504 (96.03%), Postives = 487/504 (96.63%), Query Frame = 0

Query: 7   QDQRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS------------ 66
           +  ++SRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS            
Sbjct: 2   KSSQSSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRSVSYNGLIWDEGA 61

Query: 67  ---TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSNILSTKS 126
              TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSNILSTKS
Sbjct: 62  NTMTESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSNILSTKS 121

Query: 127 KFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSAGDPDSL 186
           KFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSAGDPDSL
Sbjct: 122 KFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSAGDPDSL 181

Query: 187 SMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGSFSFQNGMQT 246
           SMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGSFSFQNGMQT
Sbjct: 182 SMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGSFSFQNGMQT 241

Query: 247 LTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADAVIMTAP 306
           LTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADAVIMTAP
Sbjct: 242 LTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADAVIMTAP 301

Query: 307 VCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNG 366
           VCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNG
Sbjct: 302 VCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNG 361

Query: 367 LRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQLLGVEG 426
           LRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQLLGVEG
Sbjct: 362 LRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQLLGVEG 421

Query: 427 EPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKA 486
           EPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKA
Sbjct: 422 EPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKA 481

Query: 487 ADLVISYLESSTDQPCAEQQELKK 496
           ADLVISYLESSTDQPCAEQQELKK
Sbjct: 482 ADLVISYLESSTDQPCAEQQELKK 505

BLAST of CmaCh15G000850 vs. ExPASy TrEMBL
Match: A0A6J1FEP1 (Protoporphyrinogen oxidase OS=Cucurbita moschata OX=3662 GN=LOC111444814 PE=3 SV=1)

HSP 1 Score: 947.2 bits (2447), Expect = 2.7e-272
Identity = 484/510 (94.90%), Postives = 490/510 (96.08%), Query Frame = 0

Query: 1   MASPAKQDQRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS------ 60
           MASPAKQDQR SRKRVAVVGAGVSGLAAAYKLK+HGF+VTVLEADGRAGGKLRS      
Sbjct: 1   MASPAKQDQRVSRKRVAVVGAGVSGLAAAYKLKSHGFSVTVLEADGRAGGKLRSVSYNGL 60

Query: 61  ---------TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSN 120
                    TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPI LIKSN
Sbjct: 61  IWDEGANTMTESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPIALIKSN 120

Query: 121 ILSTKSKFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSA 180
           ILSTKSKFQIILEPFLWKKY+TSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSA
Sbjct: 121 ILSTKSKFQIILEPFLWKKYSTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSA 180

Query: 181 GDPDSLSMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGSFSF 240
           GDPDSLSMSHSFPELWNIEKRFGSIL+GLV SKLSTKKEN GVRNGTTGKRKPTRGSFSF
Sbjct: 181 GDPDSLSMSHSFPELWNIEKRFGSILSGLVQSKLSTKKENSGVRNGTTGKRKPTRGSFSF 240

Query: 241 QNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADA 300
           QNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADA
Sbjct: 241 QNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADA 300

Query: 301 VIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVLVPS 360
           VIMTAPVCNVREMKFMKGGIPFPLNFLPEVAY+PLSVMITTFRKESVKRPLEGFGVLVPS
Sbjct: 301 VIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYLPLSVMITTFRKESVKRPLEGFGVLVPS 360

Query: 361 LEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQ 420
           LEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQ
Sbjct: 361 LEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQ 420

Query: 421 LLGVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVGKSI 480
           LLGVEGEPTFVNHFYWSKAFPLYGRNYDSV+KAIETMEKSLPGFFYAGNHRDGLSVGKSI
Sbjct: 421 LLGVEGEPTFVNHFYWSKAFPLYGRNYDSVVKAIETMEKSLPGFFYAGNHRDGLSVGKSI 480

Query: 481 ASGCKAADLVISYLESSTDQPCAEQQELKK 496
           ASGCKAADLVISYLESST QPCAEQQELKK
Sbjct: 481 ASGCKAADLVISYLESSTHQPCAEQQELKK 510

BLAST of CmaCh15G000850 vs. ExPASy TrEMBL
Match: A0A6J1FEN6 (Protoporphyrinogen oxidase OS=Cucurbita moschata OX=3662 GN=LOC111444814 PE=3 SV=1)

HSP 1 Score: 941.8 bits (2433), Expect = 1.2e-270
Identity = 485/518 (93.63%), Postives = 491/518 (94.79%), Query Frame = 0

Query: 1   MASPAKQDQRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS------ 60
           MASPAKQDQRTSRKRVAVVGAGVSGLAAAYKLK+HGF+VTVLEADGRAGGKLRS      
Sbjct: 1   MASPAKQDQRTSRKRVAVVGAGVSGLAAAYKLKSHGFSVTVLEADGRAGGKLRSVSYNGL 60

Query: 61  ---------TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSN 120
                    TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPI LIKSN
Sbjct: 61  IWDEGANTMTESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPIALIKSN 120

Query: 121 ILSTKSK--------FQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLID 180
           ILSTKSK        FQIILEPFLWKKY+TSKVSEDDTDESVGGFFQRHFGKEVVDYLID
Sbjct: 121 ILSTKSKVCFYLTIEFQIILEPFLWKKYSTSKVSEDDTDESVGGFFQRHFGKEVVDYLID 180

Query: 181 PIVAGTSAGDPDSLSMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRK 240
           PIVAGTSAGDPDSLSMSHSFPELWNIEKRFGSIL+GLV SKLSTKKEN GVRNGTTGKRK
Sbjct: 181 PIVAGTSAGDPDSLSMSHSFPELWNIEKRFGSILSGLVQSKLSTKKENSGVRNGTTGKRK 240

Query: 241 PTRGSFSFQNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSK 300
           PTRGSFSFQNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSK
Sbjct: 241 PTRGSFSFQNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSK 300

Query: 301 NLIADADAVIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLE 360
           NLIADADAVIMTAPVCNVREMKFMKGGIPFPLNFLPEVAY+PLSVMITTFRKESVKRPLE
Sbjct: 301 NLIADADAVIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYLPLSVMITTFRKESVKRPLE 360

Query: 361 GFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQ 420
           GFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQ
Sbjct: 361 GFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQ 420

Query: 421 IVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRD 480
           IVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYDSV+KAIETMEKSLPGFFYAGNHRD
Sbjct: 421 IVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYDSVVKAIETMEKSLPGFFYAGNHRD 480

Query: 481 GLSVGKSIASGCKAADLVISYLESSTDQPCAEQQELKK 496
           GLSVGKSIASGCKAADLVISYLESST QPCAEQQELKK
Sbjct: 481 GLSVGKSIASGCKAADLVISYLESSTHQPCAEQQELKK 518

BLAST of CmaCh15G000850 vs. ExPASy TrEMBL
Match: A0A6J1FKC6 (Protoporphyrinogen oxidase OS=Cucurbita moschata OX=3662 GN=LOC111444814 PE=3 SV=1)

HSP 1 Score: 939.9 bits (2428), Expect = 4.4e-270
Identity = 484/518 (93.44%), Postives = 490/518 (94.59%), Query Frame = 0

Query: 1   MASPAKQDQRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS------ 60
           MASPAKQDQR SRKRVAVVGAGVSGLAAAYKLK+HGF+VTVLEADGRAGGKLRS      
Sbjct: 1   MASPAKQDQRVSRKRVAVVGAGVSGLAAAYKLKSHGFSVTVLEADGRAGGKLRSVSYNGL 60

Query: 61  ---------TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSN 120
                    TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPI LIKSN
Sbjct: 61  IWDEGANTMTESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPIALIKSN 120

Query: 121 ILSTKSK--------FQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLID 180
           ILSTKSK        FQIILEPFLWKKY+TSKVSEDDTDESVGGFFQRHFGKEVVDYLID
Sbjct: 121 ILSTKSKVCFYLTIEFQIILEPFLWKKYSTSKVSEDDTDESVGGFFQRHFGKEVVDYLID 180

Query: 181 PIVAGTSAGDPDSLSMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRK 240
           PIVAGTSAGDPDSLSMSHSFPELWNIEKRFGSIL+GLV SKLSTKKEN GVRNGTTGKRK
Sbjct: 181 PIVAGTSAGDPDSLSMSHSFPELWNIEKRFGSILSGLVQSKLSTKKENSGVRNGTTGKRK 240

Query: 241 PTRGSFSFQNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSK 300
           PTRGSFSFQNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSK
Sbjct: 241 PTRGSFSFQNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSK 300

Query: 301 NLIADADAVIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLE 360
           NLIADADAVIMTAPVCNVREMKFMKGGIPFPLNFLPEVAY+PLSVMITTFRKESVKRPLE
Sbjct: 301 NLIADADAVIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYLPLSVMITTFRKESVKRPLE 360

Query: 361 GFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQ 420
           GFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQ
Sbjct: 361 GFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQ 420

Query: 421 IVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRD 480
           IVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYDSV+KAIETMEKSLPGFFYAGNHRD
Sbjct: 421 IVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYDSVVKAIETMEKSLPGFFYAGNHRD 480

Query: 481 GLSVGKSIASGCKAADLVISYLESSTDQPCAEQQELKK 496
           GLSVGKSIASGCKAADLVISYLESST QPCAEQQELKK
Sbjct: 481 GLSVGKSIASGCKAADLVISYLESSTHQPCAEQQELKK 518

BLAST of CmaCh15G000850 vs. NCBI nr
Match: XP_022992607.1 (protoporphyrinogen oxidase 2, chloroplastic/mitochondrial-like isoform X1 [Cucurbita maxima] >XP_022992608.1 protoporphyrinogen oxidase 2, chloroplastic/mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 967.2 bits (2499), Expect = 5.3e-278
Identity = 495/510 (97.06%), Postives = 495/510 (97.06%), Query Frame = 0

Query: 1   MASPAKQDQRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS------ 60
           MASPAKQDQRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS      
Sbjct: 1   MASPAKQDQRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRSVSYNGL 60

Query: 61  ---------TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSN 120
                    TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSN
Sbjct: 61  IWDEGANTMTESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSN 120

Query: 121 ILSTKSKFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSA 180
           ILSTKSKFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSA
Sbjct: 121 ILSTKSKFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSA 180

Query: 181 GDPDSLSMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGSFSF 240
           GDPDSLSMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGSFSF
Sbjct: 181 GDPDSLSMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGSFSF 240

Query: 241 QNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADA 300
           QNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADA
Sbjct: 241 QNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADA 300

Query: 301 VIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVLVPS 360
           VIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVLVPS
Sbjct: 301 VIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVLVPS 360

Query: 361 LEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQ 420
           LEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQ
Sbjct: 361 LEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQ 420

Query: 421 LLGVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVGKSI 480
           LLGVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVGKSI
Sbjct: 421 LLGVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVGKSI 480

Query: 481 ASGCKAADLVISYLESSTDQPCAEQQELKK 496
           ASGCKAADLVISYLESSTDQPCAEQQELKK
Sbjct: 481 ASGCKAADLVISYLESSTDQPCAEQQELKK 510

BLAST of CmaCh15G000850 vs. NCBI nr
Match: XP_022992609.1 (protoporphyrinogen oxidase 2, chloroplastic/mitochondrial-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 947.6 bits (2448), Expect = 4.3e-272
Identity = 484/504 (96.03%), Postives = 487/504 (96.63%), Query Frame = 0

Query: 7   QDQRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS------------ 66
           +  ++SRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS            
Sbjct: 2   KSSQSSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRSVSYNGLIWDEGA 61

Query: 67  ---TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSNILSTKS 126
              TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSNILSTKS
Sbjct: 62  NTMTESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSNILSTKS 121

Query: 127 KFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSAGDPDSL 186
           KFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSAGDPDSL
Sbjct: 122 KFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSAGDPDSL 181

Query: 187 SMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGSFSFQNGMQT 246
           SMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGSFSFQNGMQT
Sbjct: 182 SMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGSFSFQNGMQT 241

Query: 247 LTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADAVIMTAP 306
           LTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADAVIMTAP
Sbjct: 242 LTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADAVIMTAP 301

Query: 307 VCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNG 366
           VCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNG
Sbjct: 302 VCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVLVPSLEQQNG 361

Query: 367 LRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQLLGVEG 426
           LRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQLLGVEG
Sbjct: 362 LRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQLLGVEG 421

Query: 427 EPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKA 486
           EPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKA
Sbjct: 422 EPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVGKSIASGCKA 481

Query: 487 ADLVISYLESSTDQPCAEQQELKK 496
           ADLVISYLESSTDQPCAEQQELKK
Sbjct: 482 ADLVISYLESSTDQPCAEQQELKK 505

BLAST of CmaCh15G000850 vs. NCBI nr
Match: XP_022938652.1 (protoporphyrinogen oxidase, chloroplastic/mitochondrial isoform X8 [Cucurbita moschata])

HSP 1 Score: 947.2 bits (2447), Expect = 5.7e-272
Identity = 484/510 (94.90%), Postives = 490/510 (96.08%), Query Frame = 0

Query: 1   MASPAKQDQRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS------ 60
           MASPAKQDQR SRKRVAVVGAGVSGLAAAYKLK+HGF+VTVLEADGRAGGKLRS      
Sbjct: 1   MASPAKQDQRVSRKRVAVVGAGVSGLAAAYKLKSHGFSVTVLEADGRAGGKLRSVSYNGL 60

Query: 61  ---------TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSN 120
                    TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPI LIKSN
Sbjct: 61  IWDEGANTMTESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPIALIKSN 120

Query: 121 ILSTKSKFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSA 180
           ILSTKSKFQIILEPFLWKKY+TSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSA
Sbjct: 121 ILSTKSKFQIILEPFLWKKYSTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSA 180

Query: 181 GDPDSLSMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGSFSF 240
           GDPDSLSMSHSFPELWNIEKRFGSIL+GLV SKLSTKKEN GVRNGTTGKRKPTRGSFSF
Sbjct: 181 GDPDSLSMSHSFPELWNIEKRFGSILSGLVQSKLSTKKENSGVRNGTTGKRKPTRGSFSF 240

Query: 241 QNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADA 300
           QNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADA
Sbjct: 241 QNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADA 300

Query: 301 VIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVLVPS 360
           VIMTAPVCNVREMKFMKGGIPFPLNFLPEVAY+PLSVMITTFRKESVKRPLEGFGVLVPS
Sbjct: 301 VIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYLPLSVMITTFRKESVKRPLEGFGVLVPS 360

Query: 361 LEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQ 420
           LEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQ
Sbjct: 361 LEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQ 420

Query: 421 LLGVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVGKSI 480
           LLGVEGEPTFVNHFYWSKAFPLYGRNYDSV+KAIETMEKSLPGFFYAGNHRDGLSVGKSI
Sbjct: 421 LLGVEGEPTFVNHFYWSKAFPLYGRNYDSVVKAIETMEKSLPGFFYAGNHRDGLSVGKSI 480

Query: 481 ASGCKAADLVISYLESSTDQPCAEQQELKK 496
           ASGCKAADLVISYLESST QPCAEQQELKK
Sbjct: 481 ASGCKAADLVISYLESSTHQPCAEQQELKK 510

BLAST of CmaCh15G000850 vs. NCBI nr
Match: XP_022938647.1 (protoporphyrinogen oxidase 2, chloroplastic/mitochondrial isoform X3 [Cucurbita moschata])

HSP 1 Score: 941.8 bits (2433), Expect = 2.4e-270
Identity = 485/518 (93.63%), Postives = 491/518 (94.79%), Query Frame = 0

Query: 1   MASPAKQDQRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS------ 60
           MASPAKQDQRTSRKRVAVVGAGVSGLAAAYKLK+HGF+VTVLEADGRAGGKLRS      
Sbjct: 1   MASPAKQDQRTSRKRVAVVGAGVSGLAAAYKLKSHGFSVTVLEADGRAGGKLRSVSYNGL 60

Query: 61  ---------TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSN 120
                    TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPI LIKSN
Sbjct: 61  IWDEGANTMTESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPIALIKSN 120

Query: 121 ILSTKSK--------FQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLID 180
           ILSTKSK        FQIILEPFLWKKY+TSKVSEDDTDESVGGFFQRHFGKEVVDYLID
Sbjct: 121 ILSTKSKVCFYLTIEFQIILEPFLWKKYSTSKVSEDDTDESVGGFFQRHFGKEVVDYLID 180

Query: 181 PIVAGTSAGDPDSLSMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRK 240
           PIVAGTSAGDPDSLSMSHSFPELWNIEKRFGSIL+GLV SKLSTKKEN GVRNGTTGKRK
Sbjct: 181 PIVAGTSAGDPDSLSMSHSFPELWNIEKRFGSILSGLVQSKLSTKKENSGVRNGTTGKRK 240

Query: 241 PTRGSFSFQNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSK 300
           PTRGSFSFQNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSK
Sbjct: 241 PTRGSFSFQNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSK 300

Query: 301 NLIADADAVIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLE 360
           NLIADADAVIMTAPVCNVREMKFMKGGIPFPLNFLPEVAY+PLSVMITTFRKESVKRPLE
Sbjct: 301 NLIADADAVIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYLPLSVMITTFRKESVKRPLE 360

Query: 361 GFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQ 420
           GFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQ
Sbjct: 361 GFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQ 420

Query: 421 IVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRD 480
           IVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYDSV+KAIETMEKSLPGFFYAGNHRD
Sbjct: 421 IVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYDSVVKAIETMEKSLPGFFYAGNHRD 480

Query: 481 GLSVGKSIASGCKAADLVISYLESSTDQPCAEQQELKK 496
           GLSVGKSIASGCKAADLVISYLESST QPCAEQQELKK
Sbjct: 481 GLSVGKSIASGCKAADLVISYLESSTHQPCAEQQELKK 518

BLAST of CmaCh15G000850 vs. NCBI nr
Match: XP_022938645.1 (protoporphyrinogen oxidase 2, chloroplastic/mitochondrial isoform X2 [Cucurbita moschata])

HSP 1 Score: 939.9 bits (2428), Expect = 9.0e-270
Identity = 484/518 (93.44%), Postives = 490/518 (94.59%), Query Frame = 0

Query: 1   MASPAKQDQRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS------ 60
           MASPAKQDQR SRKRVAVVGAGVSGLAAAYKLK+HGF+VTVLEADGRAGGKLRS      
Sbjct: 1   MASPAKQDQRVSRKRVAVVGAGVSGLAAAYKLKSHGFSVTVLEADGRAGGKLRSVSYNGL 60

Query: 61  ---------TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSN 120
                    TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPI LIKSN
Sbjct: 61  IWDEGANTMTESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPIALIKSN 120

Query: 121 ILSTKSK--------FQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLID 180
           ILSTKSK        FQIILEPFLWKKY+TSKVSEDDTDESVGGFFQRHFGKEVVDYLID
Sbjct: 121 ILSTKSKVCFYLTIEFQIILEPFLWKKYSTSKVSEDDTDESVGGFFQRHFGKEVVDYLID 180

Query: 181 PIVAGTSAGDPDSLSMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRK 240
           PIVAGTSAGDPDSLSMSHSFPELWNIEKRFGSIL+GLV SKLSTKKEN GVRNGTTGKRK
Sbjct: 181 PIVAGTSAGDPDSLSMSHSFPELWNIEKRFGSILSGLVQSKLSTKKENSGVRNGTTGKRK 240

Query: 241 PTRGSFSFQNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSK 300
           PTRGSFSFQNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSK
Sbjct: 241 PTRGSFSFQNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSK 300

Query: 301 NLIADADAVIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLE 360
           NLIADADAVIMTAPVCNVREMKFMKGGIPFPLNFLPEVAY+PLSVMITTFRKESVKRPLE
Sbjct: 301 NLIADADAVIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYLPLSVMITTFRKESVKRPLE 360

Query: 361 GFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQ 420
           GFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQ
Sbjct: 361 GFGVLVPSLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQ 420

Query: 421 IVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRD 480
           IVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYDSV+KAIETMEKSLPGFFYAGNHRD
Sbjct: 421 IVTSDLRQLLGVEGEPTFVNHFYWSKAFPLYGRNYDSVVKAIETMEKSLPGFFYAGNHRD 480

Query: 481 GLSVGKSIASGCKAADLVISYLESSTDQPCAEQQELKK 496
           GLSVGKSIASGCKAADLVISYLESST QPCAEQQELKK
Sbjct: 481 GLSVGKSIASGCKAADLVISYLESSTHQPCAEQQELKK 518

BLAST of CmaCh15G000850 vs. TAIR 10
Match: AT5G14220.1 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 655.2 bits (1689), Expect = 4.1e-188
Identity = 339/502 (67.53%), Postives = 395/502 (78.69%), Query Frame = 0

Query: 1   MASPAKQD---QRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS--- 60
           MAS A  D   +  S KRVAVVGAGVSGLAAAYKLK+ G NVTV EADGR GGKLRS   
Sbjct: 1   MASGAVADHQIEAVSGKRVAVVGAGVSGLAAAYKLKSRGLNVTVFEADGRVGGKLRSVMQ 60

Query: 61  ------------TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLI 120
                       TE+EPEV  L DDLGLREKQQFPISQ KRYIVRNG+PV++PT+PI L+
Sbjct: 61  NGLIWDEGANTMTEAEPEVGSLLDDLGLREKQQFPISQKKRYIVRNGVPVMLPTNPIELV 120

Query: 121 KSNILSTKSKFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAG 180
            S++LST+SKFQI+LEPFLWKK  +SKVS+   +ESV  FFQRHFG+EVVDYLIDP V G
Sbjct: 121 TSSVLSTQSKFQILLEPFLWKK-KSSKVSDASAEESVSEFFQRHFGQEVVDYLIDPFVGG 180

Query: 181 TSAGDPDSLSMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGS 240
           TSA DPDSLSM HSFP+LWN+EK FGSI+ G + +K + K         + G +K +RGS
Sbjct: 181 TSAADPDSLSMKHSFPDLWNVEKSFGSIIVGAIRTKFAAKGGKSRDTKSSPGTKKGSRGS 240

Query: 241 FSFQNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIAD 300
           FSF+ GMQ L DTL K L  D + L S+VLSLSYN+G S  +NWSL+  S   ++     
Sbjct: 241 FSFKGGMQILPDTLCKSLSHDEINLDSKVLSLSYNSG-SRQENWSLSCVSHNETQRQNPH 300

Query: 301 ADAVIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVL 360
            DAVIMTAP+CNV+EMK MKGG PF LNFLPE+ YMPLSV+ITTF KE VKRPLEGFGVL
Sbjct: 301 YDAVIMTAPLCNVKEMKVMKGGQPFQLNFLPEINYMPLSVLITTFTKEKVKRPLEGFGVL 360

Query: 361 VPSLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSD 420
           +PS EQ++G +TLGTLFSSMMFP+R+ +D +LYTTFIGGSRN+ELAK+STDELKQ+VTSD
Sbjct: 361 IPSKEQKHGFKTLGTLFSSMMFPDRSPSDVHLYTTFIGGSRNQELAKASTDELKQVVTSD 420

Query: 421 LRQLLGVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVG 480
           L++LLGVEGEP  VNH+YW KAFPLY  +YDSVM+AI+ ME  LPGFFYAGNHR GLSVG
Sbjct: 421 LQRLLGVEGEPVSVNHYYWRKAFPLYDSSYDSVMEAIDKMENDLPGFFYAGNHRGGLSVG 480

Query: 481 KSIASGCKAADLVISYLESSTD 485
           KSIASGCKAADLVISYLES ++
Sbjct: 481 KSIASGCKAADLVISYLESCSN 500

BLAST of CmaCh15G000850 vs. TAIR 10
Match: AT5G14220.2 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 595.1 bits (1533), Expect = 5.1e-170
Identity = 319/502 (63.55%), Postives = 368/502 (73.31%), Query Frame = 0

Query: 1   MASPAKQD---QRTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRS--- 60
           MAS A  D   +  S KRVAVVGAGVSGLAAAYKLK+ G NVTV EADGR GGKLRS   
Sbjct: 1   MASGAVADHQIEAVSGKRVAVVGAGVSGLAAAYKLKSRGLNVTVFEADGRVGGKLRSVMQ 60

Query: 61  ------------TESEPEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLI 120
                       TE+EPEV  L DDLGLREKQQFPISQ KRYIVRNG+PV+         
Sbjct: 61  NGLIWDEGANTMTEAEPEVGSLLDDLGLREKQQFPISQKKRYIVRNGVPVM--------- 120

Query: 121 KSNILSTKSKFQIILEPFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAG 180
                               KK  +SKVS+   +ESV  FFQRHFG+EVVDYLIDP V G
Sbjct: 121 --------------------KK--SSKVSDASAEESVSEFFQRHFGQEVVDYLIDPFVGG 180

Query: 181 TSAGDPDSLSMSHSFPELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGKRKPTRGS 240
           TSA DPDSLSM HSFP+LWN+EK FGSI+ G + +K + K         + G +K +RGS
Sbjct: 181 TSAADPDSLSMKHSFPDLWNVEKSFGSIIVGAIRTKFAAKGGKSRDTKSSPGTKKGSRGS 240

Query: 241 FSFQNGMQTLTDTLSKELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIAD 300
           FSF+ GMQ L DTL K L  D + L S+VLSLSYN+G S  +NWSL+  S   ++     
Sbjct: 241 FSFKGGMQILPDTLCKSLSHDEINLDSKVLSLSYNSG-SRQENWSLSCVSHNETQRQNPH 300

Query: 301 ADAVIMTAPVCNVREMKFMKGGIPFPLNFLPEVAYMPLSVMITTFRKESVKRPLEGFGVL 360
            DAVIMTAP+CNV+EMK MKGG PF LNFLPE+ YMPLSV+ITTF KE VKRPLEGFGVL
Sbjct: 301 YDAVIMTAPLCNVKEMKVMKGGQPFQLNFLPEINYMPLSVLITTFTKEKVKRPLEGFGVL 360

Query: 361 VPSLEQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSD 420
           +PS EQ++G +TLGTLFSSMMFP+R+ +D +LYTTFIGGSRN+ELAK+STDELKQ+VTSD
Sbjct: 361 IPSKEQKHGFKTLGTLFSSMMFPDRSPSDVHLYTTFIGGSRNQELAKASTDELKQVVTSD 420

Query: 421 LRQLLGVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKSLPGFFYAGNHRDGLSVG 480
           L++LLGVEGEP  VNH+YW KAFPLY  +YDSVM+AI+ ME  LPGFFYAGNHR GLSVG
Sbjct: 421 LQRLLGVEGEPVSVNHYYWRKAFPLYDSSYDSVMEAIDKMENDLPGFFYAGNHRGGLSVG 470

Query: 481 KSIASGCKAADLVISYLESSTD 485
           KSIASGCKAADLVISYLES ++
Sbjct: 481 KSIASGCKAADLVISYLESCSN 470

BLAST of CmaCh15G000850 vs. TAIR 10
Match: AT4G01690.1 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 144.4 bits (363), Expect = 2.4e-34
Identity = 133/497 (26.76%), Postives = 228/497 (45.88%), Query Frame = 0

Query: 18  VVGAGVSGLAAAYKLKAH----GFNVTVLEADGRAGGKLRSTE---------------SE 77
           +VG G+SGL  A  L         N+ V EA  R GG + + E               S+
Sbjct: 61  IVGGGISGLCIAQALATKHPDAAPNLIVTEAKDRVGGNIITREENGFLWEEGPNSFQPSD 120

Query: 78  PEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSNILSTKSKFQIILE 137
           P +  + D  GL++        + R+++ NG    VP+    L   +++S   K +    
Sbjct: 121 PMLTMVVDS-GLKDDLVLGDPTAPRFVLWNGKLRPVPSKLTDLPFFDLMSIGGKIRAGFG 180

Query: 138 PFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSAGDPDSLSMSHSFP 197
                     + S    +ESV  F +R+ G EV + LI+P  +G  AGDP  LSM  +F 
Sbjct: 181 AL------GIRPSPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFG 240

Query: 198 ELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGK-RKPTRGSFSFQNGMQTLTDTLS 257
           ++W +E+  GSI+ G   +    K   +  R+    K +  T G  SF+ G++ L + +S
Sbjct: 241 KVWKLEQNGGSIIGGTFKAIQERKNAPKAERDPRLPKPQGQTVG--SFRKGLRMLPEAIS 300

Query: 258 KELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADAVIMTAPVCNVRE 317
             LG  V KL  ++  ++    K  S  ++LTY +     ++   + +V+MT P      
Sbjct: 301 ARLGSKV-KLSWKLSGIT----KLESGGYNLTYETPDGLVSV--QSKSVVMTVP------ 360

Query: 318 MKFMKGGIPFPL-----NFLPEVAYMPLSVMITTFRKESVKR------PLEGFGVLVPSL 377
              +  G+  PL     N L ++ Y P++ +  ++ KE+++        L+GFG L P  
Sbjct: 361 -SHVASGLLRPLSESAANALSKLYYPPVAAVSISYPKEAIRTECLIDGELKGFGQLHP-- 420

Query: 378 EQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQL 437
            +  G+ TLGT++SS +FPNRA     L   +IGGS N  +   S  EL + V  DLR++
Sbjct: 421 -RTQGVETLGTIYSSSLFPNRAPPGRILLLNYIGGSTNTGILSKSEGELVEAVDRDLRKM 480

Query: 438 L--GVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIET--MEKSLPGFFYAGNHRDGLSVG 480
           L      +P  +    W +A P +   +  ++   ++        G F  GN+  G+++G
Sbjct: 481 LIKPNSTDPLKLGVRVWPQAIPQFLVGHFDILDTAKSSLTSSGYEGLFLGGNYVAGVALG 531

BLAST of CmaCh15G000850 vs. TAIR 10
Match: AT4G01690.2 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 127.1 bits (318), Expect = 3.9e-29
Identity = 131/494 (26.52%), Postives = 217/494 (43.93%), Query Frame = 0

Query: 18  VVGAGVSGLAAAYKLKAH----GFNVTVLEADGRAGGKLRSTE---------------SE 77
           +VG G+SGL  A  L         N+ V EA  R GG + + E               S+
Sbjct: 61  IVGGGISGLCIAQALATKHPDAAPNLIVTEAKDRVGGNIITREENGFLWEEGPNSFQPSD 120

Query: 78  PEVQCLFDDLGLREKQQFPISQSKRYIVRNGLPVLVPTDPITLIKSNILSTKSKFQIILE 137
           P +  + D  GL++        + R+++ NG    VP+    L   +++S   K +    
Sbjct: 121 PMLTMVVDS-GLKDDLVLGDPTAPRFVLWNGKLRPVPSKLTDLPFFDLMSIGGKIRAGFG 180

Query: 138 PFLWKKYNTSKVSEDDTDESVGGFFQRHFGKEVVDYLIDPIVAGTSAGDPDSLSMSHSFP 197
                     + S    +ESV  F +R+ G EV + LI+P  +G  AGDP  LSM  +F 
Sbjct: 181 AL------GIRPSPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFG 240

Query: 198 ELWNIEKRFGSILAGLVLSKLSTKKENRGVRNGTTGK-RKPTRGSFSFQNGMQTLTDTLS 257
           ++W +E+  GSI+ G   +    K   +  R+    K +  T G  SF+ G++ L + +S
Sbjct: 241 KVWKLEQNGGSIIGGTFKAIQERKNAPKAERDPRLPKPQGQTVG--SFRKGLRMLPEAIS 300

Query: 258 KELGEDVLKLRSEVLSLSYNAGKSASQNWSLTYSSDRNSKNLIADADAVIMTAPVCNVRE 317
             LG  V KL  ++  ++    K  S  ++LTY +     ++   + +V+MT P      
Sbjct: 301 ARLGSKV-KLSWKLSGIT----KLESGGYNLTYETPDGLVSV--QSKSVVMTVP------ 360

Query: 318 MKFMKGGIPFPL-----NFLPEVAYMPLSVMITTFRKESVKR------PLEGFGVLVPSL 377
              +  G+  PL     N L ++ Y P++ +  ++ KE+++        L+GFG L P  
Sbjct: 361 -SHVASGLLRPLSESAANALSKLYYPPVAAVSISYPKEAIRTECLIDGELKGFGQLHP-- 420

Query: 378 EQQNGLRTLGTLFSSMMFPNRASNDEYLYTTFIGGSRNRELAKSSTDELKQIVTSDLRQL 437
            +  G+ TLGT++SS +FPNRA     L   +IGGS N  +   S  EL           
Sbjct: 421 -RTQGVETLGTIYSSSLFPNRAPPGRILLLNYIGGSTNTGILSKSEGELV---------- 480

Query: 438 LGVEGEPTFVNHFYWSKAFPLYGRNYDSVMKAIETMEKS-LPGFFYAGNHRDGLSVGKSI 480
                E   V HF             D +  A  ++  S   G F  GN+  G+++G+ +
Sbjct: 481 -----EAFLVGHF-------------DILDTAKSSLTSSGYEGLFLGGNYVAGVALGRCV 500

BLAST of CmaCh15G000850 vs. TAIR 10
Match: AT3G10390.1 (Flavin containing amine oxidoreductase family protein )

HSP 1 Score: 47.8 bits (112), Expect = 3.0e-05
Identity = 27/60 (45.00%), Postives = 37/60 (61.67%), Query Frame = 0

Query: 10  RTSRKRVAVVGAGVSGLAAAYKLKAHGFNVTVLEADGRAGGKLRSTESEPEVQCLFDDLG 69
           ++S+  V +VGAG+SGLAAA +L   GF VTVLE   R GG++ + + E        DLG
Sbjct: 181 QSSKSSVIIVGAGLSGLAAARQLMRFGFKVTVLEGRKRPGGRVYTKKMEANRVGAAADLG 240