Homology
BLAST of CmaCh15G000180 vs. ExPASy Swiss-Prot
Match:
F4IYF8 (Histidine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g02760 PE=2 SV=1)
HSP 1 Score: 977.6 bits (2526), Expect = 9.1e-284
Identity = 541/893 (60.58%), Postives = 664/893 (74.36%), Query Frame = 0
Query: 5 AAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHHLF-V 64
AAE S ITLGGKGSSL+S+SVY +A G + VRIDSS++ER ++ N SIK F +
Sbjct: 2 AAERSSITLGGKGSSLSSSSVYNVASGVANVRIDSSAIERFSTR----NVPSIKRSSFGI 61
Query: 65 PDYLTREEARASLVVLLNKLIIS-----PSSGIRSVIPILIAETLNSNPETLQFEPLDVT 124
P LT EE RASL VLLNKLI+S SS RSV+P+ I E LN E+L+ +DVT
Sbjct: 62 PQGLTNEETRASLAVLLNKLILSTSGPPSSSTARSVLPLKIVEILNLKAESLELGEIDVT 121
Query: 125 PEELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFA 184
E V E+SC L GIC+++DH+S LS I D+VA++SCE ++AD+++FSL+DSGDG
Sbjct: 122 EGENIVLEKSCASLIGICSIIDHKSTTLSQIVDSVAALSCEVTKADIASFSLLDSGDGNG 181
Query: 185 SKEEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKI 244
K+ +G+A D+KV LNG K GK+E ISKIP HG R K VH+ R ELNS VK
Sbjct: 182 DKDVIGVAGDLKVLLNGYKGTGKLEIEEISKIPWIHGKFRYVVKSVHADARRELNSGVKG 241
Query: 245 GKGGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRE 304
GK GS ++G +A+ T LL + +LG CS R KL DEN+K L+ ++
Sbjct: 242 GK-TGSGNTGIGEALGTTLLPLLTAIKNLGVCSFLRAKLCFEKIVDENLKKCLS---EKI 301
Query: 305 CPSNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGEL-IG 364
C NE+LK YKL L+E Y F H +N VW+IV EA+AAF + GGEL +
Sbjct: 302 CVENENLKNSYKLAYTAHLEEDYCRFAHKLNECLGIVWRIVGLEAVAAFFALAGGELFVQ 361
Query: 365 KGQDVD-INGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKL-----QGKGGGLGSLENL 424
K D D +K KKNE KKAV+GKG+S+V+Q IKD+L G + SL
Sbjct: 362 KSGDADKEESKTDKKKKKNE--KKAVVGKGTSLVIQFIKDRLVSNDAASDGDQMHSLMQC 421
Query: 425 VKAILSFLDPKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIE 484
+ IL+ +P+ FD+LL K+KEIVESN +RRLPKLPKGTRDFAKEQMA+R+KAFSII+
Sbjct: 422 GEQILNLFNPEGRSFDSLLDKVKEIVESNENRRLPKLPKGTRDFAKEQMAVREKAFSIIQ 481
Query: 485 GVFERHGATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAM 544
VF+RHGATALDTP FELRETLMGKYGEDSKLVYD+ADQGGELCSLRYDLTVPFARYVAM
Sbjct: 482 NVFKRHGATALDTPVFELRETLMGKYGEDSKLVYDIADQGGELCSLRYDLTVPFARYVAM 541
Query: 545 NGITSFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELN 604
NGITSFKRYQIAKVYRRDNPSKGR+REFYQCDFDIAG +E MGPDFE++KILTELLDEL
Sbjct: 542 NGITSFKRYQIAKVYRRDNPSKGRYREFYQCDFDIAGLFEPMGPDFEIVKILTELLDELE 601
Query: 605 IGQYEIKLNHRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEI 664
IG YE+KLNHR+LLDGMLEICGVP +KFRTICSSIDKLDKQSF+Q+K+EMVEEKGL+ EI
Sbjct: 602 IGDYEVKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSSEI 661
Query: 665 AERIGDFVKERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFD 724
A+RIG+FVKE+G PLELLSKL+QE S L N+ S +AL+EL I+F AL++S C +++VFD
Sbjct: 662 ADRIGNFVKEKGAPLELLSKLRQEGSEFLDNQSSREALDELSIMFEALKRSKCSERIVFD 721
Query: 725 LSLARGLDYYTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFV 784
LSLARGLDYYTGVI+EAV G ++GSI AGGRYDNLIGMFG+KQVPAVG+SLGIERVF
Sbjct: 722 LSLARGLDYYTGVIFEAVCI-GAEVGSIGAGGRYDNLIGMFGTKQVPAVGMSLGIERVFN 781
Query: 785 IMEQLLKDQNQTTRATKTEVLVSILGDD-LTQAAELASEIWSAKVHAEFLVNKRVMKHID 844
IME+L + Q Q R T+T+VLVSI+ D+ L +AAEL S++W AK++AE+LV+KR KH +
Sbjct: 782 IMEELNEKQKQVIRPTETQVLVSIMVDNKLAEAAELVSQLWGAKINAEYLVSKRKEKHFN 841
Query: 845 RAKEARIPWIVFLGEREASEGIVKLKNV----ETFEEVTVSRSNIIDELKKRL 880
RAKE+ IPW+V +GE+E S V LK + E E+ T +R ++ELKK L
Sbjct: 842 RAKESGIPWMVMVGEKELSGSFVTLKKLEKGSEEKEDQTCTRDRFVEELKKLL 883
BLAST of CmaCh15G000180 vs. ExPASy Swiss-Prot
Match:
P93422 (Histidine--tRNA ligase, cytoplasmic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0150900 PE=2 SV=2)
HSP 1 Score: 754.6 bits (1947), Expect = 1.3e-216
Identity = 416/760 (54.74%), Postives = 534/760 (70.26%), Query Frame = 0
Query: 139 LDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASKEEVGIANDMKVFLNGSKL 198
+D ++ L +ADAVA++SCEA+R DV+AF + SGDG ++K+E +A D+K+ L GSKL
Sbjct: 36 IDCCASPLVRVADAVAALSCEAARGDVAAFDVPTSGDGLSAKDEADVAADVKMLLFGSKL 95
Query: 199 VGK---IESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGKGGG-SLSSGTEDAVR 258
VG ++ + +K+P +G RE + +H+++R+ELN+ VK+GK G E+A+
Sbjct: 96 VGAAGGADAASFTKVPTVNGIFREAVRALHARVRIELNAPVKLGKRDAVQTGEGKEEALV 155
Query: 259 TVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECPSNESLKREYKLVSE 318
+ A + + K S+ R +L + D ++ L ++ + LK ++ +
Sbjct: 156 ALATQLARPVQAMLKLSVARARLCVARIDDAELRKKLTDGVE-----IDDLK---GMLGK 215
Query: 319 LGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQDVDINGA------- 378
+ +D + + V I+AWEA A IE I K Q + N A
Sbjct: 216 VTIDSDAVSVLRGVYNSLLKFRDILAWEAAVAMAVIEMDSSIEKPQACEENEAGSSTENP 275
Query: 379 ---NEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKA-----ILSFL 438
EK + KKK LGKG+S V+ L++D L G + S+ + + A +
Sbjct: 276 HASGEKPKGDKKSKKKKTLGKGTSAVLMLLRD-LVTNGKEVLSVNSALLAEWGTELSLLF 335
Query: 439 DPKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGA 498
DPK +L+ K+KEIVE+N RRLPK+PKGTRDF KEQMAIR+ AFSII GVF+ HGA
Sbjct: 336 DPKCPRLVSLVDKVKEIVETNEVRRLPKIPKGTRDFGKEQMAIREHAFSIITGVFKMHGA 395
Query: 499 TALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKR 558
+LDTP FELRETLMGKYGEDSKL+YDLADQGGELCSLRYDLTVPFARYVAMN I+S KR
Sbjct: 396 VSLDTPVFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNNISSLKR 455
Query: 559 YQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKL 618
YQIAKVYRRDNPSKGR+REFYQCDFDIAG YE M PDFEVIK+LTELLD+L+IG YEIKL
Sbjct: 456 YQIAKVYRRDNPSKGRYREFYQCDFDIAGVYETMEPDFEVIKVLTELLDQLDIGTYEIKL 515
Query: 619 NHRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFV 678
NHR+LLDGMLEICGVP +KFRT+CSSIDKLDKQ+F+Q+K+E+V+EKG++ E A++IGD V
Sbjct: 516 NHRKLLDGMLEICGVPPEKFRTVCSSIDKLDKQTFEQVKKELVDEKGISNETADKIGDLV 575
Query: 679 KERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLD 738
K RG PLE+L +L++E S + N GS ALNELEILF AL+K+N + K+VFDLSLARGLD
Sbjct: 576 KTRGPPLEVLLELRKEGSKFMGNAGSVTALNELEILFKALDKANAIGKIVFDLSLARGLD 635
Query: 739 YYTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKD 798
YYTGVIYEAVFKG TQ+GSIAAGGRYDNL+GMF KQVPAVGVSLGIERVF IMEQ +
Sbjct: 636 YYTGVIYEAVFKGTTQVGSIAAGGRYDNLVGMFSGKQVPAVGVSLGIERVFAIMEQQEME 695
Query: 799 QNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPW 858
+NQ RAT+TEVLVSI+G DL AAEL SE+W+A + AEF + R+ H+ A ++ IPW
Sbjct: 696 KNQ-IRATETEVLVSIIGKDLILAAELVSELWNAGIKAEFKLTTRIQNHLKYATQSGIPW 755
Query: 859 IVFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRL 880
+V +GE E S G VKLKN+ +E V R+ LK++L
Sbjct: 756 MVLVGESEISSGKVKLKNLAASQEEEVDRTEFAQVLKQKL 785
BLAST of CmaCh15G000180 vs. ExPASy Swiss-Prot
Match:
E9QI36 (Histidine--tRNA ligase OS=Danio rerio OX=7955 GN=hars PE=3 SV=2)
HSP 1 Score: 469.9 bits (1208), Expect = 6.2e-131
Identity = 246/457 (53.83%), Postives = 328/457 (71.77%), Query Frame = 0
Query: 447 KLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLVYD 506
K KGTRD+ +QMAIR+K F+II F+RHGA +D+P FEL+ETL GKYGEDSKL+YD
Sbjct: 57 KTAKGTRDYNPKQMAIREKVFNIIINCFKRHGAETIDSPVFELKETLTGKYGEDSKLIYD 116
Query: 507 LADQGGELCSLRYDLTVPFARYVAMNGITSFKRYQIAKVYRRDNP--SKGRFREFYQCDF 566
L DQGGEL SLRYDLTVPFARY+AMN IT+ KRY IAKVYRRDNP ++GR+REFYQCDF
Sbjct: 117 LKDQGGELLSLRYDLTVPFARYLAMNKITNIKRYHIAKVYRRDNPAMTRGRYREFYQCDF 176
Query: 567 DIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLNHRRLLDGMLEICGVPSDKFRTICS 626
DIAGQY+ M PD E +K++ E+L EL++G + IK+N RR+LDGM ICGVP +KFRTICS
Sbjct: 177 DIAGQYDAMIPDAECLKLVYEILSELDLGDFRIKVNDRRILDGMFAICGVPDEKFRTICS 236
Query: 627 SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG 686
++DKLDK +++++K+EMV EKGL+ E+A+RI D+V +G +L +L Q+ L Q+K
Sbjct: 237 TVDKLDKLAWEEVKKEMVNEKGLSEEVADRIRDYVSMQGGK-DLAERLLQDPK-LSQSKQ 296
Query: 687 SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVF-------------- 746
+ + ++++LFS LE DKVVFDLSLARGLDYYTGVIYEA+
Sbjct: 297 ACAGITDMKLLFSYLELFQITDKVVFDLSLARGLDYYTGVIYEAILTQANPAPASTPAEQ 356
Query: 747 ----KGGTQIGSIAAGGRYDNLIGMFGSK--QVPAVGVSLGIERVFVIMEQLLKDQNQTT 806
G +GS+A GGRYD L+GMF K +VP VGVS+GIERVF IMEQ + ++
Sbjct: 357 NGAEDAGVSVGSVAGGGRYDGLVGMFDPKGRKVPCVGVSIGIERVFSIMEQKAELSSEKV 416
Query: 807 RATKTEVLV-SILGDDLTQAAELASEIWSAKVHAEFLV--NKRVMKHIDRAKEARIPWIV 866
R T+T+VLV S + L + +L +E+W+A + AE L N +++ + ++ IP +
Sbjct: 417 RTTETQVLVASAQKNLLEERLKLTAELWNAGIKAEVLYKKNPKLLSQLQHCEDTGIPLVA 476
Query: 867 FLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKR 879
LGE+E +G+VKL+NV + EEV V R+ ++DE+KKR
Sbjct: 477 ILGEQELKDGVVKLRNVASREEVDVPRAELVDEVKKR 511
BLAST of CmaCh15G000180 vs. ExPASy Swiss-Prot
Match:
Q2KI84 (Histidine--tRNA ligase, cytoplasmic OS=Bos taurus OX=9913 GN=HARS1 PE=2 SV=1)
HSP 1 Score: 469.2 bits (1206), Expect = 1.1e-130
Identity = 242/447 (54.14%), Postives = 325/447 (72.71%), Query Frame = 0
Query: 447 KLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLVYD 506
K PKGTRD++ QMA+R+K F +I F+RHGA +DTP FEL+ETL GKYGEDSKL+YD
Sbjct: 57 KTPKGTRDYSPRQMAVREKVFDVIISCFKRHGAEVIDTPVFELKETLTGKYGEDSKLIYD 116
Query: 507 LADQGGELCSLRYDLTVPFARYVAMNGITSFKRYQIAKVYRRDNP--SKGRFREFYQCDF 566
L DQGGEL SLRYDLTVPFARY+AMN +T+ KRY IAKVYRRDNP ++GR+REFYQCDF
Sbjct: 117 LKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDF 176
Query: 567 DIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLNHRRLLDGMLEICGVPSDKFRTICS 626
DIAGQ++ M PD E +KI+ E+L L IG + +K+N RR+LDGM ICGVP KFRTICS
Sbjct: 177 DIAGQFDPMLPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVPDSKFRTICS 236
Query: 627 SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG 686
S+DKLDK S++++K EMV EKGL E+A+RIGD+V++ G + L+ +L Q+ L QNK
Sbjct: 237 SVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHG-GVSLVEQLLQDPK-LSQNKQ 296
Query: 687 SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVF-----KGGTQ---I 746
+ + L +L++LF L DK+ FDLSLARGLDYYTGVIYEAV + G + +
Sbjct: 297 ALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQPPARAGEEPLGV 356
Query: 747 GSIAAGGRYDNLIGMFGSK--QVPAVGVSLGIERVFVIMEQLLKDQNQTTRATKTEVLV- 806
GS+AAGGRYD L+GMF K +VP VG+S+G+ER+F I+EQ L+ + R T+T+VLV
Sbjct: 357 GSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKVRTTETQVLVA 416
Query: 807 SILGDDLTQAAELASEIWSAKVHAEFLV--NKRVMKHIDRAKEARIPWIVFLGEREASEG 866
S L + +L SE+W A + AE L N +++ + +E IP + +GE+E +G
Sbjct: 417 SAQKKLLEERLKLISELWDAGIKAELLYKKNPKLLNQLQYCEETGIPLVAIIGEQELKDG 476
Query: 867 IVKLKNVETFEEVTVSRSNIIDELKKR 879
++KL++V + EEV V R ++++E+K+R
Sbjct: 477 VIKLRSVASREEVDVRREDLVEEIKRR 501
BLAST of CmaCh15G000180 vs. ExPASy Swiss-Prot
Match:
Q61035 (Histidine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Hars1 PE=1 SV=2)
HSP 1 Score: 467.6 bits (1202), Expect = 3.1e-130
Identity = 242/447 (54.14%), Postives = 326/447 (72.93%), Query Frame = 0
Query: 447 KLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLVYD 506
K PKGTRD++ QMA+R+K F +I F+RHGA +DTP FEL+ETL GKYGEDSKL+YD
Sbjct: 57 KTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLTGKYGEDSKLIYD 116
Query: 507 LADQGGELCSLRYDLTVPFARYVAMNGITSFKRYQIAKVYRRDNP--SKGRFREFYQCDF 566
L DQGGEL SLRYDLTVPFARY+AMN +T+ KRY IAKVYRRDNP ++GR+REFYQCDF
Sbjct: 117 LKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDF 176
Query: 567 DIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLNHRRLLDGMLEICGVPSDKFRTICS 626
DIAGQ++ M PD E +KI+ E+L L IG + +K+N RR+LDGM +CGVP KFRTICS
Sbjct: 177 DIAGQFDPMIPDAECLKIMCEILSSLQIGNFLVKVNDRRILDGMFAVCGVPDSKFRTICS 236
Query: 627 SIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLSKLKQEQSPLLQNKG 686
S+DKLDK S++++K EMV EKGL E+A+RIGD+V++ G + L+ +L Q+ L QNK
Sbjct: 237 SVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHG-GVSLVEQLLQDPK-LSQNKQ 296
Query: 687 SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVF-----KGGTQ---I 746
+ + L +L++LF L DK+ FDLSLARGLDYYTGVIYEAV + G + +
Sbjct: 297 AVEGLGDLKLLFEYLILFGIDDKISFDLSLARGLDYYTGVIYEAVLLQMPTQAGEEPLGV 356
Query: 747 GSIAAGGRYDNLIGMFGSK--QVPAVGVSLGIERVFVIMEQLLKDQNQTTRATKTEVLV- 806
GSIAAGGRYD L+GMF K +VP VG+S+G+ER+F I+EQ L+ + R T+T+VLV
Sbjct: 357 GSIAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEASEEKVRTTETQVLVA 416
Query: 807 SILGDDLTQAAELASEIWSAKVHAEFLV--NKRVMKHIDRAKEARIPWIVFLGEREASEG 866
S L + +L SE+W A + AE L N +++ + +EA IP + +GE+E +G
Sbjct: 417 SAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDG 476
Query: 867 IVKLKNVETFEEVTVSRSNIIDELKKR 879
++KL++V + EEV V R ++++E+++R
Sbjct: 477 VIKLRSVASREEVDVRREDLVEEIRRR 501
BLAST of CmaCh15G000180 vs. ExPASy TrEMBL
Match:
A0A6J1JV84 (Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111489180 PE=3 SV=1)
HSP 1 Score: 1680.2 bits (4350), Expect = 0.0e+00
Identity = 881/881 (100.00%), Postives = 881/881 (100.00%), Query Frame = 0
Query: 1 MAHPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MAHPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH
Sbjct: 1 MAHPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
Query: 61 LFVPDYLTREEARASLVVLLNKLIISPSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
LFVPDYLTREEARASLVVLLNKLIISPSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE
Sbjct: 61 LFVPDYLTREEARASLVVLLNKLIISPSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
Query: 121 ELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180
ELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK
Sbjct: 121 ELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK
Sbjct: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP
Sbjct: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
Query: 301 SNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
SNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ
Sbjct: 301 SNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKAILSFLD 420
DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKAILSFLD
Sbjct: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKAILSFLD 420
Query: 421 PKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 480
PKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT
Sbjct: 421 PKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 480
Query: 481 ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY 540
ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY
Sbjct: 481 ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY 540
Query: 541 QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN 600
QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN
Sbjct: 541 QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN 600
Query: 601 HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK 660
HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK
Sbjct: 601 HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK 660
Query: 661 ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY 720
ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY
Sbjct: 661 ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY 720
Query: 721 YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ 780
YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ
Sbjct: 721 YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ 780
Query: 781 NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI 840
NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI
Sbjct: 781 NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI 840
Query: 841 VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLAP 882
VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLAP
Sbjct: 841 VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLAP 881
BLAST of CmaCh15G000180 vs. ExPASy TrEMBL
Match:
A0A6J1FE95 (Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111444724 PE=3 SV=1)
HSP 1 Score: 1655.2 bits (4285), Expect = 0.0e+00
Identity = 865/881 (98.18%), Postives = 875/881 (99.32%), Query Frame = 0
Query: 1 MAHPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MA PAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH
Sbjct: 1 MADPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
Query: 61 LFVPDYLTREEARASLVVLLNKLIISPSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
LFVPD+LTR+EARASLVVLLNKLIIS SSGIRSVIPILIAETLNSNPETLQFEPLDVTPE
Sbjct: 61 LFVPDFLTRDEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
Query: 121 ELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180
ELSVFEQSCY+L+GICALLDHQSAALSSIADAVASISCEASRADVSAFSL+DSGDGFASK
Sbjct: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVGIANDMKVFLNGSKLVGKIESGAIS IPKTHGCLREQAKLVHSKMRVELNSSVKIGK
Sbjct: 181 EEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP
Sbjct: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
Query: 301 SNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
S+ESLKREY+LVSELGLDEKY+EFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ
Sbjct: 301 SSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKAILSFLD 420
DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVK +LSFLD
Sbjct: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD 420
Query: 421 PKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 480
PKASGFDNLL+KIKEIVESN SRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT
Sbjct: 421 PKASGFDNLLRKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 480
Query: 481 ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY 540
ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY
Sbjct: 481 ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY 540
Query: 541 QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN 600
QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN
Sbjct: 541 QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN 600
Query: 601 HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK 660
HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK
Sbjct: 601 HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK 660
Query: 661 ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY 720
ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY
Sbjct: 661 ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY 720
Query: 721 YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ 780
YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ
Sbjct: 721 YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ 780
Query: 781 NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI 840
NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI
Sbjct: 781 NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI 840
Query: 841 VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLAP 882
VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRL P
Sbjct: 841 VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLTP 881
BLAST of CmaCh15G000180 vs. ExPASy TrEMBL
Match:
A0A6J1DGB6 (Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111020184 PE=3 SV=1)
HSP 1 Score: 1523.5 bits (3943), Expect = 0.0e+00
Identity = 799/886 (90.18%), Postives = 841/886 (94.92%), Query Frame = 0
Query: 1 MAHPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MA P +E+SVITLGGKGSSL+S+SVYAIAHGF+LVRIDSS+LERL+SSSN+ NAAS KH
Sbjct: 1 MADP-SEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQ 60
Query: 61 LFVPDYLTREEARASLVVLLNKLIISPSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
+F+P++LTREEARASL VLLNKLIIS SSGIRSVIP+LIAETLNS PETLQFE LDVT E
Sbjct: 61 VFIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDE 120
Query: 121 ELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180
ELSVF+QSCYVL+GICALLDHQSAALSSIADAVA+ISCEASRADVSAF LMDSGDGFASK
Sbjct: 121 ELSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVG+ANDMKV LNGSKLVGKIE GA SKIPK HGCLREQAKLVHSKMRVELNS +K GK
Sbjct: 181 EEVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGSLSSGTED+VRT LLSFAA+LWDLGKCSL R KLIL SN +EN+KASL GLLDRECP
Sbjct: 241 -GGSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECP 300
Query: 301 SNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
SNESLK+EYKLVSEL LD++Y EFVHTVNVL TVWKIVAWEAIAAF+TIEGGEL+GKGQ
Sbjct: 301 SNESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKG-----GGLGSLENLVKAI 420
DVDIN NEKVVKKNEKKKKAVLGKG+SVVVQLIKD+LQGKG GGLGSLEN VK +
Sbjct: 361 DVDINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDL 420
Query: 421 LSFLDPKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFE 480
LSFLDPKAS FDNLLKKIKEIVESN SRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFE
Sbjct: 421 LSFLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFE 480
Query: 481 RHGATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGIT 540
RHGATALDTPAFELRETLMGKYGEDSKL+YDLADQGGELCSLRYDLTVPFARYVAMNGIT
Sbjct: 481 RHGATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGIT 540
Query: 541 SFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQY 600
SFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IGQY
Sbjct: 541 SFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQY 600
Query: 601 EIKLNHRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERI 660
EIKLNHR+LLDGMLEICGVP +KFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAE+I
Sbjct: 601 EIKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKI 660
Query: 661 GDFVKERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLA 720
GDFVKERGHPLELLSKLKQEQ PLLQNKGS DAL ELEILF+ALEKS CVDKVVFDLSLA
Sbjct: 661 GDFVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLA 720
Query: 721 RGLDYYTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ 780
RGLDYYTGVIYEAVFKGGTQ+GSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ
Sbjct: 721 RGLDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ 780
Query: 781 LLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEA 840
LLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAK++AEFLVNKRVMKHIDRAKE+
Sbjct: 781 LLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKES 840
Query: 841 RIPWIVFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLAP 882
RIPWIVFLGERE SEGIVKLKNVETFEEVT+SRSNIIDELKKRL+P
Sbjct: 841 RIPWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884
BLAST of CmaCh15G000180 vs. ExPASy TrEMBL
Match:
A0A6J1GRC1 (Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111456820 PE=3 SV=1)
HSP 1 Score: 1516.5 bits (3925), Expect = 0.0e+00
Identity = 791/881 (89.78%), Postives = 838/881 (95.12%), Query Frame = 0
Query: 1 MAHPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MA P AE+SVITLGGKGSSL+S+S++AIAHGF+LVRIDSS+LERLASSSNN NAAS+KH
Sbjct: 1 MADP-AEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQ 60
Query: 61 LFVPDYLTREEARASLVVLLNKLIISPSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
+ +P+YLTREEARASLVVLLNKLIIS SSGIRSVIP+LIAETLNS PETLQFE LDVT E
Sbjct: 61 VVIPNYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDE 120
Query: 121 ELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180
ELSVF+QSCYVL+G+CALLDHQSAALSSIADAVA ISCEASRADVS F+LMDSGDGFASK
Sbjct: 121 ELSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVG+ANDMKV LNGSKLVGKIESGAISKIPK HGCLREQAKLVHSKMRVELNS+VKIGK
Sbjct: 181 EEVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGSLSSGTED VRT LLSFAA+LWDLGKCSLDR KLI SN D+NMKASLAGLLDRECP
Sbjct: 241 -GGSLSSGTEDTVRTALLSFAAILWDLGKCSLDRGKLIFDSNSDDNMKASLAGLLDRECP 300
Query: 301 SNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
SNESLK+ Y+LVS+L LD+ Y EFVHTVNVL TVWKIV WEAIA F+TIEGG+LIGKGQ
Sbjct: 301 SNESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKAILSFLD 420
DVD+N ANEKVV K+EKKKKAVLGKG+SVVVQLIKD+LQGKGG LG LEN VK ILSFLD
Sbjct: 361 DVDMNRANEKVVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLG-LENSVKDILSFLD 420
Query: 421 PKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 480
PKAS FDNLL KI+EIVESN SRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT
Sbjct: 421 PKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 480
Query: 481 ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY 540
ALDTPAFELRETLMGKYGEDSKL+YDL+DQGGELCSLRYDLTVPFARYVAMNGITSFKRY
Sbjct: 481 ALDTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYVAMNGITSFKRY 540
Query: 541 QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN 600
QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN
Sbjct: 541 QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN 600
Query: 601 HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK 660
HR+LLDGMLEICGVP +KFRTICSSIDKLDKQSFDQIKREMVEEKGL +EIAERIGDFVK
Sbjct: 601 HRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVK 660
Query: 661 ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY 720
ERGHPLELL+KLKQEQSPLLQNKGSS AL+ELEILFSALEK+ CVDKVVFDLSLARGLDY
Sbjct: 661 ERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARGLDY 720
Query: 721 YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ 780
YTGVIYEA+FKGGTQ+GSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ
Sbjct: 721 YTGVIYEAIFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ 780
Query: 781 NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI 840
NQTTRATKTE+LVSILGDDLTQAAELASEIWSAK++AEFL+NKRVMKHIDRAKE+RIPWI
Sbjct: 781 NQTTRATKTEILVSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRIPWI 840
Query: 841 VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLAP 882
VFLGERE SEGIVKLKNVETFEEVT+ RSNIIDELKKRLAP
Sbjct: 841 VFLGEREVSEGIVKLKNVETFEEVTILRSNIIDELKKRLAP 878
BLAST of CmaCh15G000180 vs. ExPASy TrEMBL
Match:
A0A6J1JT52 (Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111487614 PE=3 SV=1)
HSP 1 Score: 1511.9 bits (3913), Expect = 0.0e+00
Identity = 787/881 (89.33%), Postives = 837/881 (95.01%), Query Frame = 0
Query: 1 MAHPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MA P AE+SVITLGGKGSSL+S+S++AIAHGF+LVRIDSS+LERLASSSNN NAAS+KH
Sbjct: 1 MADP-AEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQ 60
Query: 61 LFVPDYLTREEARASLVVLLNKLIISPSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
+ +PDYLTREEARASLVVLLNKLIIS SSGIRSVIP+LIAETLNS PETLQFE LDVT E
Sbjct: 61 VVIPDYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDE 120
Query: 121 ELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180
ELSVF+QSCYVL+G+CALLDHQSAALSSIADAVA ISCEASRADVS F+LMDSGDGFASK
Sbjct: 121 ELSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVG+ANDMKV LNGSKLVGKIESGAISKIPK HGCLREQAKLVHSKMRVELNS+VKIGK
Sbjct: 181 EEVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGS+SSGTED VRT LLSFAAMLWDLGKCSLDR KLIL SN D+NMKASLAGLLDRECP
Sbjct: 241 -GGSMSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECP 300
Query: 301 SNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
SNESLK+ Y+LVS+L LD+ Y EFVHTVNVL TVWKIV WEAIA F+TIEGG+LIGKGQ
Sbjct: 301 SNESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKAILSFLD 420
DVD+N ANEKV+ K+EKKKKAVLGKG+SVVVQLIKD+LQG+ G LG LEN VK +LSFLD
Sbjct: 361 DVDMNRANEKVLNKSEKKKKAVLGKGTSVVVQLIKDRLQGESGDLG-LENSVKDLLSFLD 420
Query: 421 PKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 480
PKAS FDNLL KI+EIVESN SRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT
Sbjct: 421 PKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 480
Query: 481 ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY 540
ALDTPAFELRETLMGKYGEDSKL+YDL+DQGGELCSLRYDLTVPFARY+AMNGITSFKRY
Sbjct: 481 ALDTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYIAMNGITSFKRY 540
Query: 541 QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN 600
QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN
Sbjct: 541 QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN 600
Query: 601 HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK 660
HR+LLDGMLEICGVP +KFRTICSSIDKLDKQSFDQIKREMVEEKGL +EIAERIGDFVK
Sbjct: 601 HRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVK 660
Query: 661 ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY 720
ERGHPLELL+KLKQEQSPLLQNKGSS AL+ELEILFSALEK+ CVDKVVFDLSLARGLDY
Sbjct: 661 ERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARGLDY 720
Query: 721 YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ 780
YTGVIYEA+FKGGTQ+GSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ LKDQ
Sbjct: 721 YTGVIYEAIFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQHLKDQ 780
Query: 781 NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI 840
NQTTRATKTE+LVSILGDDLTQAAELASEIWSAK++AEFL+NKRVMKHIDRAKE+RIPWI
Sbjct: 781 NQTTRATKTEILVSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRIPWI 840
Query: 841 VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLAP 882
VFLGERE SEGIVKLKNVETFEEVT+ RSNIIDELKKRLAP
Sbjct: 841 VFLGEREVSEGIVKLKNVETFEEVTILRSNIIDELKKRLAP 878
BLAST of CmaCh15G000180 vs. NCBI nr
Match:
XP_022993046.1 (histidine--tRNA ligase, cytoplasmic-like [Cucurbita maxima])
HSP 1 Score: 1680.2 bits (4350), Expect = 0.0e+00
Identity = 881/881 (100.00%), Postives = 881/881 (100.00%), Query Frame = 0
Query: 1 MAHPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MAHPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH
Sbjct: 1 MAHPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
Query: 61 LFVPDYLTREEARASLVVLLNKLIISPSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
LFVPDYLTREEARASLVVLLNKLIISPSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE
Sbjct: 61 LFVPDYLTREEARASLVVLLNKLIISPSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
Query: 121 ELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180
ELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK
Sbjct: 121 ELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK
Sbjct: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP
Sbjct: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
Query: 301 SNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
SNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ
Sbjct: 301 SNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKAILSFLD 420
DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKAILSFLD
Sbjct: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKAILSFLD 420
Query: 421 PKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 480
PKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT
Sbjct: 421 PKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 480
Query: 481 ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY 540
ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY
Sbjct: 481 ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY 540
Query: 541 QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN 600
QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN
Sbjct: 541 QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN 600
Query: 601 HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK 660
HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK
Sbjct: 601 HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK 660
Query: 661 ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY 720
ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY
Sbjct: 661 ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY 720
Query: 721 YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ 780
YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ
Sbjct: 721 YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ 780
Query: 781 NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI 840
NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI
Sbjct: 781 NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI 840
Query: 841 VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLAP 882
VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLAP
Sbjct: 841 VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLAP 881
BLAST of CmaCh15G000180 vs. NCBI nr
Match:
XP_022938504.1 (histidine--tRNA ligase, cytoplasmic-like [Cucurbita moschata] >KAG6578315.1 Histidine--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1655.2 bits (4285), Expect = 0.0e+00
Identity = 865/881 (98.18%), Postives = 875/881 (99.32%), Query Frame = 0
Query: 1 MAHPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MA PAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH
Sbjct: 1 MADPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
Query: 61 LFVPDYLTREEARASLVVLLNKLIISPSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
LFVPD+LTR+EARASLVVLLNKLIIS SSGIRSVIPILIAETLNSNPETLQFEPLDVTPE
Sbjct: 61 LFVPDFLTRDEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
Query: 121 ELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180
ELSVFEQSCY+L+GICALLDHQSAALSSIADAVASISCEASRADVSAFSL+DSGDGFASK
Sbjct: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLLDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVGIANDMKVFLNGSKLVGKIESGAIS IPKTHGCLREQAKLVHSKMRVELNSSVKIGK
Sbjct: 181 EEVGIANDMKVFLNGSKLVGKIESGAISNIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP
Sbjct: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
Query: 301 SNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
S+ESLKREY+LVSELGLDEKY+EFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ
Sbjct: 301 SSESLKREYRLVSELGLDEKYVEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKAILSFLD 420
DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVK +LSFLD
Sbjct: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD 420
Query: 421 PKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 480
PKASGFDNLL+KIKEIVESN SRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT
Sbjct: 421 PKASGFDNLLRKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 480
Query: 481 ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY 540
ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY
Sbjct: 481 ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY 540
Query: 541 QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN 600
QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN
Sbjct: 541 QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN 600
Query: 601 HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK 660
HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK
Sbjct: 601 HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK 660
Query: 661 ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY 720
ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY
Sbjct: 661 ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY 720
Query: 721 YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ 780
YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ
Sbjct: 721 YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ 780
Query: 781 NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI 840
NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI
Sbjct: 781 NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI 840
Query: 841 VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLAP 882
VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRL P
Sbjct: 841 VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLTP 881
BLAST of CmaCh15G000180 vs. NCBI nr
Match:
XP_023550553.1 (histidine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1653.3 bits (4280), Expect = 0.0e+00
Identity = 864/881 (98.07%), Postives = 873/881 (99.09%), Query Frame = 0
Query: 1 MAHPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MA PAAELSVITLGGKGSSLASTSVYAIAHGF+LVRIDSSSLER++SSSNNPNAASIKHH
Sbjct: 1 MADPAAELSVITLGGKGSSLASTSVYAIAHGFALVRIDSSSLERISSSSNNPNAASIKHH 60
Query: 61 LFVPDYLTREEARASLVVLLNKLIISPSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
LFVPD+LTREEARASLVVLLNKLIIS SSGIRSVIPILIAETLNSNPETLQFEPLDVTPE
Sbjct: 61 LFVPDFLTREEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
Query: 121 ELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180
ELSVFEQSCY+L+GICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK
Sbjct: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK
Sbjct: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLI GSNGDENMKASLAGLLDRECP
Sbjct: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLIWGSNGDENMKASLAGLLDRECP 300
Query: 301 SNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
SNESLKREY+LVSELGLDEKY+EFVH VNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ
Sbjct: 301 SNESLKREYRLVSELGLDEKYVEFVHAVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKAILSFLD 420
DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVK +LSFLD
Sbjct: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD 420
Query: 421 PKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 480
PKASGFDNLLKKIKEIVESN SRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT
Sbjct: 421 PKASGFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 480
Query: 481 ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY 540
ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY
Sbjct: 481 ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY 540
Query: 541 QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN 600
QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIG YEIKLN
Sbjct: 541 QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGHYEIKLN 600
Query: 601 HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK 660
HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK
Sbjct: 601 HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK 660
Query: 661 ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY 720
ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY
Sbjct: 661 ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY 720
Query: 721 YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ 780
YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ
Sbjct: 721 YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ 780
Query: 781 NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI 840
NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI
Sbjct: 781 NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI 840
Query: 841 VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLAP 882
VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRL P
Sbjct: 841 VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLTP 881
BLAST of CmaCh15G000180 vs. NCBI nr
Match:
XP_022152462.1 (histidine--tRNA ligase, cytoplasmic [Momordica charantia])
HSP 1 Score: 1523.5 bits (3943), Expect = 0.0e+00
Identity = 799/886 (90.18%), Postives = 841/886 (94.92%), Query Frame = 0
Query: 1 MAHPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MA P +E+SVITLGGKGSSL+S+SVYAIAHGF+LVRIDSS+LERL+SSSN+ NAAS KH
Sbjct: 1 MADP-SEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQ 60
Query: 61 LFVPDYLTREEARASLVVLLNKLIISPSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
+F+P++LTREEARASL VLLNKLIIS SSGIRSVIP+LIAETLNS PETLQFE LDVT E
Sbjct: 61 VFIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDE 120
Query: 121 ELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180
ELSVF+QSCYVL+GICALLDHQSAALSSIADAVA+ISCEASRADVSAF LMDSGDGFASK
Sbjct: 121 ELSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVG+ANDMKV LNGSKLVGKIE GA SKIPK HGCLREQAKLVHSKMRVELNS +K GK
Sbjct: 181 EEVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGSLSSGTED+VRT LLSFAA+LWDLGKCSL R KLIL SN +EN+KASL GLLDRECP
Sbjct: 241 -GGSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECP 300
Query: 301 SNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
SNESLK+EYKLVSEL LD++Y EFVHTVNVL TVWKIVAWEAIAAF+TIEGGEL+GKGQ
Sbjct: 301 SNESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKG-----GGLGSLENLVKAI 420
DVDIN NEKVVKKNEKKKKAVLGKG+SVVVQLIKD+LQGKG GGLGSLEN VK +
Sbjct: 361 DVDINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDL 420
Query: 421 LSFLDPKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFE 480
LSFLDPKAS FDNLLKKIKEIVESN SRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFE
Sbjct: 421 LSFLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFE 480
Query: 481 RHGATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGIT 540
RHGATALDTPAFELRETLMGKYGEDSKL+YDLADQGGELCSLRYDLTVPFARYVAMNGIT
Sbjct: 481 RHGATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGIT 540
Query: 541 SFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQY 600
SFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDEL+IGQY
Sbjct: 541 SFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQY 600
Query: 601 EIKLNHRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERI 660
EIKLNHR+LLDGMLEICGVP +KFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAE+I
Sbjct: 601 EIKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKI 660
Query: 661 GDFVKERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLA 720
GDFVKERGHPLELLSKLKQEQ PLLQNKGS DAL ELEILF+ALEKS CVDKVVFDLSLA
Sbjct: 661 GDFVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLA 720
Query: 721 RGLDYYTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ 780
RGLDYYTGVIYEAVFKGGTQ+GSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ
Sbjct: 721 RGLDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQ 780
Query: 781 LLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEA 840
LLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAK++AEFLVNKRVMKHIDRAKE+
Sbjct: 781 LLKDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKES 840
Query: 841 RIPWIVFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLAP 882
RIPWIVFLGERE SEGIVKLKNVETFEEVT+SRSNIIDELKKRL+P
Sbjct: 841 RIPWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884
BLAST of CmaCh15G000180 vs. NCBI nr
Match:
KAG6602728.1 (Histidine--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1520.8 bits (3936), Expect = 0.0e+00
Identity = 793/881 (90.01%), Postives = 839/881 (95.23%), Query Frame = 0
Query: 1 MAHPAAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHH 60
MA P AE+SVITLGGKGSSL+S+S++AIAHGF+LVRIDSS+LERLASSSNN NAAS+KH
Sbjct: 1 MADP-AEVSVITLGGKGSSLSSSSIHAIAHGFALVRIDSSALERLASSSNNRNAASVKHQ 60
Query: 61 LFVPDYLTREEARASLVVLLNKLIISPSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
+ +PDYLTREEARASLVVLLNKLIIS SSGIRSVIP+LIAETLNS PETLQFE LDVT E
Sbjct: 61 VVIPDYLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDE 120
Query: 121 ELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180
ELSVF+QSCYVL+G+CALLDHQSAALSSIADAVA ISCEASRADVS F+LMDSGDGFASK
Sbjct: 121 ELSVFKQSCYVLNGVCALLDHQSAALSSIADAVAGISCEASRADVSVFNLMDSGDGFASK 180
Query: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
EEVG+ANDMKV LNGSKLVGKIESGAISKIPK HGCLREQAKLVHSKMRVELNS+VKIGK
Sbjct: 181 EEVGVANDMKVLLNGSKLVGKIESGAISKIPKNHGCLREQAKLVHSKMRVELNSNVKIGK 240
Query: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRECP 300
GGSLSSGTED VRT LLSFAAMLWDLGKCSLDR KLIL SN D+NMKASLAGLLDRECP
Sbjct: 241 -GGSLSSGTEDTVRTALLSFAAMLWDLGKCSLDRGKLILDSNSDDNMKASLAGLLDRECP 300
Query: 301 SNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
SNESLK+ Y+LVS+L LD+ Y EFVHTVNVL TVWKIV WEAIA F+TIEGG+LIGKGQ
Sbjct: 301 SNESLKKVYRLVSDLALDDNYDEFVHTVNVLLLTVWKIVVWEAIAGFLTIEGGKLIGKGQ 360
Query: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKAILSFLD 420
DVD+N ANEKVV K+EKKKKAVLGKG+SVVVQLIKD+LQGKGG LG LEN VK +LSFLD
Sbjct: 361 DVDMNRANEKVVNKSEKKKKAVLGKGTSVVVQLIKDRLQGKGGDLG-LENSVKDLLSFLD 420
Query: 421 PKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 480
PKAS FDNLL KI+EIVESN SRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT
Sbjct: 421 PKASEFDNLLMKIREIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 480
Query: 481 ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY 540
ALDTPAFELRETLMGKYGEDSKL+YDL+DQGGELCSLRYDLTVPFARYVAMNGITSFKRY
Sbjct: 481 ALDTPAFELRETLMGKYGEDSKLIYDLSDQGGELCSLRYDLTVPFARYVAMNGITSFKRY 540
Query: 541 QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN 600
QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN
Sbjct: 541 QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGQYEIKLN 600
Query: 601 HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK 660
HR+LLDGMLEICGVP +KFRTICSSIDKLDKQSFDQIKREMVEEKGL +EIAERIGDFVK
Sbjct: 601 HRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLALEIAERIGDFVK 660
Query: 661 ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY 720
ERGHPLELL+KLKQEQSPLLQNKGSS AL+ELEILFSALEK+ CVDKVVFDLSLARGLDY
Sbjct: 661 ERGHPLELLAKLKQEQSPLLQNKGSSGALSELEILFSALEKAKCVDKVVFDLSLARGLDY 720
Query: 721 YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ 780
YTGVIYEA+FKGGTQ+GSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ
Sbjct: 721 YTGVIYEAIFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ 780
Query: 781 NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI 840
NQTTRATKTE+LVSILGDDLTQAAELASEIWSAK++AEFL+NKRVMKHIDRAKE+RIPWI
Sbjct: 781 NQTTRATKTEILVSILGDDLTQAAELASEIWSAKLNAEFLINKRVMKHIDRAKESRIPWI 840
Query: 841 VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLAP 882
VFLGERE SEGIVKLKNVETFEEVT+ RSNIIDELKKRLAP
Sbjct: 841 VFLGEREVSEGIVKLKNVETFEEVTILRSNIIDELKKRLAP 878
BLAST of CmaCh15G000180 vs. TAIR 10
Match:
AT3G02760.1 (Class II aaRS and biotin synthetases superfamily protein )
HSP 1 Score: 977.6 bits (2526), Expect = 6.5e-285
Identity = 541/893 (60.58%), Postives = 664/893 (74.36%), Query Frame = 0
Query: 5 AAELSVITLGGKGSSLASTSVYAIAHGFSLVRIDSSSLERLASSSNNPNAASIKHHLF-V 64
AAE S ITLGGKGSSL+S+SVY +A G + VRIDSS++ER ++ N SIK F +
Sbjct: 2 AAERSSITLGGKGSSLSSSSVYNVASGVANVRIDSSAIERFSTR----NVPSIKRSSFGI 61
Query: 65 PDYLTREEARASLVVLLNKLIIS-----PSSGIRSVIPILIAETLNSNPETLQFEPLDVT 124
P LT EE RASL VLLNKLI+S SS RSV+P+ I E LN E+L+ +DVT
Sbjct: 62 PQGLTNEETRASLAVLLNKLILSTSGPPSSSTARSVLPLKIVEILNLKAESLELGEIDVT 121
Query: 125 PEELSVFEQSCYVLSGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFA 184
E V E+SC L GIC+++DH+S LS I D+VA++SCE ++AD+++FSL+DSGDG
Sbjct: 122 EGENIVLEKSCASLIGICSIIDHKSTTLSQIVDSVAALSCEVTKADIASFSLLDSGDGNG 181
Query: 185 SKEEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKI 244
K+ +G+A D+KV LNG K GK+E ISKIP HG R K VH+ R ELNS VK
Sbjct: 182 DKDVIGVAGDLKVLLNGYKGTGKLEIEEISKIPWIHGKFRYVVKSVHADARRELNSGVKG 241
Query: 245 GKGGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLILGSNGDENMKASLAGLLDRE 304
GK GS ++G +A+ T LL + +LG CS R KL DEN+K L+ ++
Sbjct: 242 GK-TGSGNTGIGEALGTTLLPLLTAIKNLGVCSFLRAKLCFEKIVDENLKKCLS---EKI 301
Query: 305 CPSNESLKREYKLVSELGLDEKYIEFVHTVNVLFATVWKIVAWEAIAAFMTIEGGEL-IG 364
C NE+LK YKL L+E Y F H +N VW+IV EA+AAF + GGEL +
Sbjct: 302 CVENENLKNSYKLAYTAHLEEDYCRFAHKLNECLGIVWRIVGLEAVAAFFALAGGELFVQ 361
Query: 365 KGQDVD-INGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKL-----QGKGGGLGSLENL 424
K D D +K KKNE KKAV+GKG+S+V+Q IKD+L G + SL
Sbjct: 362 KSGDADKEESKTDKKKKKNE--KKAVVGKGTSLVIQFIKDRLVSNDAASDGDQMHSLMQC 421
Query: 425 VKAILSFLDPKASGFDNLLKKIKEIVESNASRRLPKLPKGTRDFAKEQMAIRKKAFSIIE 484
+ IL+ +P+ FD+LL K+KEIVESN +RRLPKLPKGTRDFAKEQMA+R+KAFSII+
Sbjct: 422 GEQILNLFNPEGRSFDSLLDKVKEIVESNENRRLPKLPKGTRDFAKEQMAVREKAFSIIQ 481
Query: 485 GVFERHGATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAM 544
VF+RHGATALDTP FELRETLMGKYGEDSKLVYD+ADQGGELCSLRYDLTVPFARYVAM
Sbjct: 482 NVFKRHGATALDTPVFELRETLMGKYGEDSKLVYDIADQGGELCSLRYDLTVPFARYVAM 541
Query: 545 NGITSFKRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELN 604
NGITSFKRYQIAKVYRRDNPSKGR+REFYQCDFDIAG +E MGPDFE++KILTELLDEL
Sbjct: 542 NGITSFKRYQIAKVYRRDNPSKGRYREFYQCDFDIAGLFEPMGPDFEIVKILTELLDELE 601
Query: 605 IGQYEIKLNHRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEI 664
IG YE+KLNHR+LLDGMLEICGVP +KFRTICSSIDKLDKQSF+Q+K+EMVEEKGL+ EI
Sbjct: 602 IGDYEVKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSSEI 661
Query: 665 AERIGDFVKERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFD 724
A+RIG+FVKE+G PLELLSKL+QE S L N+ S +AL+EL I+F AL++S C +++VFD
Sbjct: 662 ADRIGNFVKEKGAPLELLSKLRQEGSEFLDNQSSREALDELSIMFEALKRSKCSERIVFD 721
Query: 725 LSLARGLDYYTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFV 784
LSLARGLDYYTGVI+EAV G ++GSI AGGRYDNLIGMFG+KQVPAVG+SLGIERVF
Sbjct: 722 LSLARGLDYYTGVIFEAVCI-GAEVGSIGAGGRYDNLIGMFGTKQVPAVGMSLGIERVFN 781
Query: 785 IMEQLLKDQNQTTRATKTEVLVSILGDD-LTQAAELASEIWSAKVHAEFLVNKRVMKHID 844
IME+L + Q Q R T+T+VLVSI+ D+ L +AAEL S++W AK++AE+LV+KR KH +
Sbjct: 782 IMEELNEKQKQVIRPTETQVLVSIMVDNKLAEAAELVSQLWGAKINAEYLVSKRKEKHFN 841
Query: 845 RAKEARIPWIVFLGEREASEGIVKLKNV----ETFEEVTVSRSNIIDELKKRL 880
RAKE+ IPW+V +GE+E S V LK + E E+ T +R ++ELKK L
Sbjct: 842 RAKESGIPWMVMVGEKELSGSFVTLKKLEKGSEEKEDQTCTRDRFVEELKKLL 883
BLAST of CmaCh15G000180 vs. TAIR 10
Match:
AT3G46100.1 (Histidyl-tRNA synthetase 1 )
HSP 1 Score: 159.8 bits (403), Expect = 9.7e-39
Identity = 125/432 (28.94%), Postives = 215/432 (49.77%), Query Frame = 0
Query: 449 PKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSK-LVYDL 508
PKGTRDF E M +R F+ + V +G +D P E + K GE+ + +Y
Sbjct: 72 PKGTRDFPPEDMRLRNWLFNHFKEVSRLYGYEEVDYPVLETEALFIRKAGEEIRDQLYCF 131
Query: 509 ADQGGELCSLRYDLTVPFARYVAMNGIT---SFKRYQIAKVYRRDNPSKGRFREFYQCDF 568
D+G +LR +LT AR V G + K + I + +R + ++GR RE YQ +
Sbjct: 132 EDRGNRRVALRPELTPSLARLVIQKGKSVSLPLKWFAIGQCWRYERMTRGRRREHYQWNM 191
Query: 569 DIAGQYEKMGPDFEVIKILTELLDELNIGQYEI--KLNHRRLLDGMLEICGVPSDKFRTI 628
DI G ++ + E+I + + I ++ K++ R++L +L+ GVP D F +
Sbjct: 192 DIIG-VPQVTAEAELISSIVTFFKRIGITASDVGFKVSSRKVLQELLKKYGVPEDLFGRV 251
Query: 629 CSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVKERGHPLELLS-KLKQEQSPLLQ 688
C IDK++K D+IK+E+ G++ + E++ L++LS K + ++
Sbjct: 252 CIIIDKIEKIPIDEIKKEL-GFTGISEDAIEQL----------LQVLSVKSLDDLEDII- 311
Query: 689 NKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQIGSIAA 748
G+ +A+ +L+ LFS EK + + FD S+ RGL YYTG+++E + G ++ +I
Sbjct: 312 -GGAGEAIADLKQLFSLAEKFGYSEWIQFDASVVRGLAYYTGIVFEGFDRKG-KLRAICG 371
Query: 749 GGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQNQTTRATKTEVLVSILGDDLT 808
GGRYD L+ +G PA G G VI+E L + + E +V L DL
Sbjct: 372 GGRYDRLLSTYGGDDFPACGFGFGDA---VIVELLKEKDLLPELGQEVENIVCALDKDLQ 431
Query: 809 -QAAELASEIWSAKVHAEFLVNKRVMKHI-DRAKEARIPWIVFLGEREASEGIVKLKNVE 868
AA +A+ + + ++ + +K + RA +V +G+ E +G V +K +
Sbjct: 432 GAAATVATALRDKGQTVDLVLESKPLKWVFKRAARVNARRLVLVGKTEWEDGSVSVKVLS 485
Query: 869 TFEEVTVSRSNI 872
+ E+ V S++
Sbjct: 492 SGEQFQVKLSDL 485
BLAST of CmaCh15G000180 vs. TAIR 10
Match:
AT3G59410.1 (protein kinase family protein )
HSP 1 Score: 117.1 bits (292), Expect = 7.2e-26
Identity = 117/454 (25.77%), Postives = 205/454 (45.15%), Query Frame = 0
Query: 462 IRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDL 521
+R I + VF +H A L+ + L+ + S+ L GG++ L Y+L
Sbjct: 796 LRDYVVEITKEVFRQHCAKHLEV----IPMRLLSDCPQFSRKTVKLLTNGGDMLELCYEL 855
Query: 522 TVPFARYVAMNGITSFKRYQIAKVYRR------DNPSKGRFREFYQCDFDIAGQYEKMGP 581
+PF ++++N +SFKRY+I+ VYRR NP Q DFDI G +
Sbjct: 856 RLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPC-------LQADFDIVGGTLSL-T 915
Query: 582 DFEVIKILTELLDEL-NIGQYEIKLNHRRLLDGMLEICGVPSD---KFRTICSSIDKLDK 641
+ EV+K++ ++ + + G +I LNH LLD + G+ ++ K + S + L
Sbjct: 916 EAEVLKVIVDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRP 975
Query: 642 QSFDQ------IKREMVEEKGLTVEIAERIGDFVKE-RGHPLELLSKLKQEQSPLLQNKG 701
QS ++ I+R++++E L + R+ G + L +L+ L ++
Sbjct: 976 QSSERKLKWVFIRRQLLQELKLPEAVVNRLQTVASRFCGDADQALPRLR---GALRADRP 1035
Query: 702 SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQIGS------ 761
+ AL+EL L + L + V D+ + Y+ + ++ G+
Sbjct: 1036 TRKALDELSNLLTYLRVWRIEEHVHIDVLMPPTESYHRNLFFQVFLTKENSSGTSNDGVL 1095
Query: 762 IAAGGRYDNLIGMFGSKQ----VP-AVGVSLGIERVFVIMEQLLKDQNQTTRATKTEVLV 821
+A GGRYD L+ ++ +P AVGVSL +E +F + L D T VLV
Sbjct: 1096 LAVGGRYDWLVQEVCDREHKMNLPGAVGVSLALETIF---QHLPMDLRPIRNEVSTSVLV 1155
Query: 822 SILGDD--LTQAAELASEIWSAKVHAEFL--VNKRVMKHIDRAKEARIPWIVFLGEREAS 881
G L Q EL +E+W + AEF+ + + + + A E I +V + E +
Sbjct: 1156 CSRGGGGLLVQRMELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITESGVA 1215
BLAST of CmaCh15G000180 vs. TAIR 10
Match:
AT3G59410.2 (protein kinase family protein )
HSP 1 Score: 117.1 bits (292), Expect = 7.2e-26
Identity = 117/454 (25.77%), Postives = 205/454 (45.15%), Query Frame = 0
Query: 462 IRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDL 521
+R I + VF +H A L+ + L+ + S+ L GG++ L Y+L
Sbjct: 820 LRDYVVEITKEVFRQHCAKHLEV----IPMRLLSDCPQFSRKTVKLLTNGGDMLELCYEL 879
Query: 522 TVPFARYVAMNGITSFKRYQIAKVYRR------DNPSKGRFREFYQCDFDIAGQYEKMGP 581
+PF ++++N +SFKRY+I+ VYRR NP Q DFDI G +
Sbjct: 880 RLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPC-------LQADFDIVGGTLSL-T 939
Query: 582 DFEVIKILTELLDEL-NIGQYEIKLNHRRLLDGMLEICGVPSD---KFRTICSSIDKLDK 641
+ EV+K++ ++ + + G +I LNH LLD + G+ ++ K + S + L
Sbjct: 940 EAEVLKVIVDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRP 999
Query: 642 QSFDQ------IKREMVEEKGLTVEIAERIGDFVKE-RGHPLELLSKLKQEQSPLLQNKG 701
QS ++ I+R++++E L + R+ G + L +L+ L ++
Sbjct: 1000 QSSERKLKWVFIRRQLLQELKLPEAVVNRLQTVASRFCGDADQALPRLR---GALRADRP 1059
Query: 702 SSDALNELEILFSALEKSNCVDKVVFDLSLARGLDYYTGVIYEAVFKGGTQIGS------ 761
+ AL+EL L + L + V D+ + Y+ + ++ G+
Sbjct: 1060 TRKALDELSNLLTYLRVWRIEEHVHIDVLMPPTESYHRNLFFQVFLTKENSSGTSNDGVL 1119
Query: 762 IAAGGRYDNLIGMFGSKQ----VP-AVGVSLGIERVFVIMEQLLKDQNQTTRATKTEVLV 821
+A GGRYD L+ ++ +P AVGVSL +E +F + L D T VLV
Sbjct: 1120 LAVGGRYDWLVQEVCDREHKMNLPGAVGVSLALETIF---QHLPMDLRPIRNEVSTSVLV 1179
Query: 822 SILGDD--LTQAAELASEIWSAKVHAEFL--VNKRVMKHIDRAKEARIPWIVFLGEREAS 881
G L Q EL +E+W + AEF+ + + + + A E I +V + E +
Sbjct: 1180 CSRGGGGLLVQRMELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITESGVA 1239
BLAST of CmaCh15G000180 vs. TAIR 10
Match:
AT5G03406.1 (Class II aaRS and biotin synthetases superfamily protein )
HSP 1 Score: 87.4 bits (215), Expect = 6.1e-17
Identity = 58/187 (31.02%), Postives = 92/187 (49.20%), Query Frame = 0
Query: 449 PKGTRDFAKEQMAIRKKAFSIIEGVFERHGATALDTPAFELRETLMGKYGEDSK-LVYDL 508
PKGTRDF E M +R F+ + V G +D P E + K GE+ + +Y
Sbjct: 72 PKGTRDFPPEDMRLRNWLFNHFKEVSRLFGYEEVDFPVLETEALFIRKAGEEIRDQLYCF 131
Query: 509 ADQGGELCSLRYDLTVPFARYVAMNGIT---SFKRYQIAKVYRRDNPSKGRFREFYQCDF 568
D+G +LR +LT AR V G + K + + + +R + ++GR RE YQ +
Sbjct: 132 EDRGNRRVALRPELTPSLARLVIQKGKSVSLPLKWFAVGQCWRYERMTRGRRREHYQWNM 191
Query: 569 DIAGQYEKMGPDFEVIKILTELLDELNIGQYEI--KLNHRRLLDGMLEICGVPSDKFRTI 628
DI G E + + E+I + + I ++ K++ R++L L+ GVP D F +
Sbjct: 192 DIIGVPE-VTAEAELISSIMTFFKRIGITASDVGFKVSSRKVLQEFLKKYGVPEDLFGRV 251
Query: 629 CSSIDKL 630
C IDK+
Sbjct: 252 CFIIDKV 257
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| F4IYF8 | 9.1e-284 | 60.58 | Histidine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g02760... | [more] |
| P93422 | 1.3e-216 | 54.74 | Histidine--tRNA ligase, cytoplasmic OS=Oryza sativa subsp. japonica OX=39947 GN=... | [more] |
| E9QI36 | 6.2e-131 | 53.83 | Histidine--tRNA ligase OS=Danio rerio OX=7955 GN=hars PE=3 SV=2 | [more] |
| Q2KI84 | 1.1e-130 | 54.14 | Histidine--tRNA ligase, cytoplasmic OS=Bos taurus OX=9913 GN=HARS1 PE=2 SV=1 | [more] |
| Q61035 | 3.1e-130 | 54.14 | Histidine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Hars1 PE=1 SV=2 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1JV84 | 0.0e+00 | 100.00 | Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111489180 PE=3 SV=1 | [more] |
| A0A6J1FE95 | 0.0e+00 | 98.18 | Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111444724 PE=3 SV=1 | [more] |
| A0A6J1DGB6 | 0.0e+00 | 90.18 | Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111020184 PE=3 SV=1 | [more] |
| A0A6J1GRC1 | 0.0e+00 | 89.78 | Histidine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111456820 PE=3 SV=1 | [more] |
| A0A6J1JT52 | 0.0e+00 | 89.33 | Histidine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111487614 PE=3 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_022993046.1 | 0.0e+00 | 100.00 | histidine--tRNA ligase, cytoplasmic-like [Cucurbita maxima] | [more] |
| XP_022938504.1 | 0.0e+00 | 98.18 | histidine--tRNA ligase, cytoplasmic-like [Cucurbita moschata] >KAG6578315.1 Hist... | [more] |
| XP_023550553.1 | 0.0e+00 | 98.07 | histidine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo] | [more] |
| XP_022152462.1 | 0.0e+00 | 90.18 | histidine--tRNA ligase, cytoplasmic [Momordica charantia] | [more] |
| KAG6602728.1 | 0.0e+00 | 90.01 | Histidine--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. soro... | [more] |
