CmaCh14G021190 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh14G021190
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionPyridoxal 5'-phosphate synthase
LocationCma_Chr14: 14591068 .. 14593255 (+)
RNA-Seq ExpressionCmaCh14G021190
SyntenyCmaCh14G021190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAAATTACTGAATTTCGCTTACTATTTCTCAATGATGTACCGAACTGCGAATACATAGAGCAGAGCAGTAGGCGAATACATAGAGCAGAGCAGTAGGCGAATTAGCGGGCAATTAGGAAATGGGATCGGCAGCGGCGCCATGGAAGTCCCTTCTTCTCAGTGCTTTGGAATCTAATGCCCAAATCACGCATTCCAGATTCTTGCACCTCGTACATTTCCCTATCTCTATTATCTTCAACTTTAACTCCCCTTTCCCTTTTCCACAGTCGGGTTTTATCTTTTAAATGGAATTTGTTTTCAGGCAACGATTGGAGGCAATGGAAGGCCATCAAATCGCACCGTTGTTTTCAGAGGATTTGAAGAGGGCACCGATGGGATCCGTATTTACACGGATTCAAGAAGTCGCAAGGTTCTTCTACGTCGAAGCTCTATATTTCGTTTTAGATCGCTGAATTCATACGCCCACTTCTTGTTGTTCTATGGTTTCCGACGCAATCGTTGTCTGGGTTTCTGGATTTTCTGGATTTTCTGGATTTTCAATTATGTAATTCATATGTTTGGCTACAATAAGATAAGCTATAAAATATGCTCAGTACTGGGAATTTGTCTGCGATGGATTAGGTCAGGTTTGTGATATTAAGCATCTGAGTTTTGTCCTGCAAATGAAGTTTTCACTTTTGGTTGATTCTGCAGATTGAAGAGCTTAAAACTTGTCCATTTGCTGAGGTAACTATTCTCGTCTCTTCCTGCCTCTTAATACAGTTCGTAATACAAATGATTTGTTCTTGCTGGTCTATGTAGCTTATCTTATTTACATGACTGCAAATATGTGCCAATTTGAGTCAAATTTTGGACTTGGAATGTTTTGTTCTTCAAGATAAGTTGGTATTTCACCGAATCATGGGAGCAATTTCGTATCAGTGGAAGAATTGAGGTTGTTGATGCGTCATGCCTCGATGCTACAAAATTTAAGGTTGTGGGCATGACCCTTTCCTCTTATAACTTACAACTTGTTCTAAATTTTCAACAGTTCACTTTCTTGTTCACTTCGTTCAACTCAGTATAATCTTATATATTTATTAATGCAGAAAAGAGCAGAAGCCTGGTCCTCCATTTCTCCAAAGTCAAGAATGCAGTATTTGGGGCCTAGTCCAAATCTTCCCTATATTGCCGAACAGCCAGCCAAGGAACCTGTTCTAGATTCTTGTTCAGGTCCTGTTGATGCATTTTGTTTGCTGGTCTTGGATCCTGATCAGGTTTGTAATATATATAGACATATTTCTCTAGATTAGTATATGACCTTGTTCTTTTACCTTATGTTACATTTGACCGCGTTTCGCTCTCAGGTCGATTACTTGAATCTGAAGAGTGCAGAAAGGAAGTTGTTTAAAGCTATTCCATGTCTTGGAAGGGAGACATTATGGGAATCAGAGCGGATAAACCCTTAGCAATTGCACCTCGATTTGCAGGTATATTGATAACAACAAGCTGCTATCTTGAAATCAACTGATATAGTTAGTTACTGATGTGTTGAAAATAATCCTCCGACCCTTTGGTCGTTAGTAGAGATGATATTGGTTGATATCTAGTTCTTTGTTAGTAGAGATGATATTGGTTGATATCTAGTTCTTTTTGCCCGACTCCCATGGCCATATCTGTATGTAACTCAATTCTTATGCTCTTCAACCTGAAACAAAAGGAATGAAAAGTTGGAATGAGTTAGTTGTTTGAAGCATGAAATATGCAAACGAACAGAAGGATAGGAAAGGGTCGGTGTTTGTTGGGTGTAAGGGAAATGAAACATTATCGGATAACGCCCAGGAGTTGCAGAAGTGTTTTTCCTATCTGAAGCCAGTCGTTTTCATGGAAAGGATAACCCAACAACACCAAACACAAAGATTGTCTTCAATCCCTACGCGCATTGCTTGGGTCCCACCGCTTCCGTCCCACGTCCCATCAAACTAAATTATATACCCCTCTCTGACTCTAATGCTCTATCGGACCTTTCATCAAAGTTTTAGAGGACATCAACACCCTTGTAAGGGATGTTTAATCTAATTTTGTGTTTAAGAGTTATGTGCGTGATGTATGAGACACGTCCTTACAAAATTACGTACGACTAGTTAATCTAGAGAGCGTTGAAATTAATATTTGAACACCAATAAGAATGAAGGTTAATGGGATTAG

mRNA sequence

GTAAATTACTGAATTTCGCTTACTATTTCTCAATGATGTACCGAACTGCGAATACATAGAGCAGAGCAGTAGGCGAATACATAGAGCAGAGCAGTAGGCGAATTAGCGGGCAATTAGGAAATGGGATCGGCAGCGGCGCCATGGAAGTCCCTTCTTCTCAGTGCTTTGGAATCTAATGCCCAAATCACGCATTCCAGATTCTTGCACCTCGCAACGATTGGAGGCAATGGAAGGCCATCAAATCGCACCGTTGTTTTCAGAGGATTTGAAGAGGGCACCGATGGGATCCGTATTTACACGGATTCAAGAAGTCGCAAGATTGAAGAGCTTAAAACTTGTCCATTTGCTGAGATAAGTTGGTATTTCACCGAATCATGGGAGCAATTTCGTATCAGTGGAAGAATTGAGGTTGTTGATGCGTCATGCCTCGATGCTACAAAATTTAAGAAAAGAGCAGAAGCCTGGTCCTCCATTTCTCCAAAGTCAAGAATGCAGTATTTGGGGCCTAGTCCAAATCTTCCCTATATTGCCGAACAGCCAGCCAAGGAACCTGTTCTAGATTCTTGTTCAGGTCCTGTTGATGCATTTTGTTTGCTGGTCTTGGATCCTGATCAGGTCGATTACTTGAATCTGAAGAGTGCAGAAAGGAAGTTGTTTAAAGCTATTCCATGTCTTGGAAGGGAGACATTATGGGAATCAGAGCGGATAAACCCTTAGCAATTGCACCTCGATTTGCAGGTATATTGATAACAACAAGCTGCTATCTTGAAATCAACTGATATAGTTAGTTACTGATGTGTTGAAAATAATCCTCCGACCCTTTGGTCGTTAGTAGAGATGATATTGGTTGATATCTAGTTCTTTGTTAGTAGAGATGATATTGGTTGATATCTAGTTCTTTTTGCCCGACTCCCATGGCCATATCTGTATGTAACTCAATTCTTATGCTCTTCAACCTGAAACAAAAGGAATGAAAAGTTGGAATGAGTTAGTTGTTTGAAGCATGAAATATGCAAACGAACAGAAGGATAGGAAAGGGTCGGTGTTTGTTGGGTGTAAGGGAAATGAAACATTATCGGATAACGCCCAGGAGTTGCAGAAGTGTTTTTCCTATCTGAAGCCAGTCGTTTTCATGGAAAGGATAACCCAACAACACCAAACACAAAGATTGTCTTCAATCCCTACGCGCATTGCTTGGGTCCCACCGCTTCCGTCCCACGTCCCATCAAACTAAATTATATACCCCTCTCTGACTCTAATGCTCTATCGGACCTTTCATCAAAGTTTTAGAGGACATCAACACCCTTGTAAGGGATGTTTAATCTAATTTTGTGTTTAAGAGTTATGTGCGTGATGTATGAGACACGTCCTTACAAAATTACGTACGACTAGTTAATCTAGAGAGCGTTGAAATTAATATTTGAACACCAATAAGAATGAAGGTTAATGGGATTAG

Coding sequence (CDS)

ATGGGATCGGCAGCGGCGCCATGGAAGTCCCTTCTTCTCAGTGCTTTGGAATCTAATGCCCAAATCACGCATTCCAGATTCTTGCACCTCGCAACGATTGGAGGCAATGGAAGGCCATCAAATCGCACCGTTGTTTTCAGAGGATTTGAAGAGGGCACCGATGGGATCCGTATTTACACGGATTCAAGAAGTCGCAAGATTGAAGAGCTTAAAACTTGTCCATTTGCTGAGATAAGTTGGTATTTCACCGAATCATGGGAGCAATTTCGTATCAGTGGAAGAATTGAGGTTGTTGATGCGTCATGCCTCGATGCTACAAAATTTAAGAAAAGAGCAGAAGCCTGGTCCTCCATTTCTCCAAAGTCAAGAATGCAGTATTTGGGGCCTAGTCCAAATCTTCCCTATATTGCCGAACAGCCAGCCAAGGAACCTGTTCTAGATTCTTGTTCAGGTCCTGTTGATGCATTTTGTTTGCTGGTCTTGGATCCTGATCAGGTCGATTACTTGAATCTGAAGAGTGCAGAAAGGAAGTTGTTTAAAGCTATTCCATGTCTTGGAAGGGAGACATTATGGGAATCAGAGCGGATAAACCCTTAG

Protein sequence

MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYTDSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFKAIPCLGRETLWESERINP
Homology
BLAST of CmaCh14G021190 vs. ExPASy Swiss-Prot
Match: Q9ZPY1 (Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=PPOX2 PE=1 SV=2)

HSP 1 Score: 234.6 bits (597), Expect = 9.8e-61
Identity = 110/198 (55.56%), Postives = 150/198 (75.76%), Query Frame = 0

Query: 1   MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT 60
           MG+  APWK LL  A+E+N+ ++HS ++ LATIG NGRPSNRTVVFRGFEE +D I+I T
Sbjct: 1   MGTHVAPWKQLLFGAIEANSHLSHSSYVQLATIGLNGRPSNRTVVFRGFEENSDRIQINT 60

Query: 61  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 120
           D RSRKIEELK CPF+E+ WYF+++WEQFRI+GRIEV+DAS  D TK ++R +AW + S 
Sbjct: 61  DLRSRKIEELKHCPFSEMCWYFSDTWEQFRINGRIEVIDASNPDQTKLQQREKAWFANSL 120

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 180
           +SR+ Y+ P+P  P  +EQ +++  LD  SGPV  +CLL+L+P++VDYLNLK+ +R  F 
Sbjct: 121 RSRLIYVCPTPGSPCNSEQSSQQVKLDPSSGPVPEYCLLLLEPEKVDYLNLKTNQRLFFS 180

Query: 181 AIPCLGRETLWESERINP 199
           ++     E  W SE++NP
Sbjct: 181 SMATGTGEKCWTSEKVNP 198

BLAST of CmaCh14G021190 vs. ExPASy Swiss-Prot
Match: Q7P203 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) OX=243365 GN=pdxH PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 4.3e-08
Identity = 34/102 (33.33%), Postives = 56/102 (54.90%), Query Frame = 0

Query: 20  AQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYTDSRSRKIEELKTCPFAEIS 79
           AQ+     ++LA IG +GRPS+R V+ +G E+G   +  YT+ +SRK + L+  P+  ++
Sbjct: 40  AQVPEPTAMNLAAIGADGRPSSRIVLLKGVEDGQ--LLFYTNYQSRKGQALEANPYVALN 99

Query: 80  WYFTESWEQFRISGRIEVVDASCLDA----TKFKKRAEAWSS 118
           +++ E   Q RI G+   V     DA      +  R  AW+S
Sbjct: 100 FFWPELERQVRIEGKAARVAPEVSDAYFASRPYTSRLGAWAS 139

BLAST of CmaCh14G021190 vs. ExPASy Swiss-Prot
Match: A1TQY3 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Acidovorax citrulli (strain AAC00-1) OX=397945 GN=pdxH PE=3 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.3e-07
Identity = 37/102 (36.27%), Postives = 54/102 (52.94%), Query Frame = 0

Query: 20  AQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYTDSRSRKIEELKTCPFAEIS 79
           AQ+     + LAT+G + RPS R V+ +G++E   GI  YT+  SRK  +L   PFA + 
Sbjct: 52  AQVPEPNAMTLATVGADLRPSTRVVLIKGYDE--RGIVWYTNYGSRKGRQLAGNPFAALQ 111

Query: 80  WYFTESWEQFRISGRIEVVDASCLDA----TKFKKRAEAWSS 118
           +++ E     RI GR+E V  +  DA         R  AW+S
Sbjct: 112 FHWVELERVVRIEGRVEKVSDAESDAYFASRPLDSRIGAWAS 151

BLAST of CmaCh14G021190 vs. ExPASy Swiss-Prot
Match: B1YVG1 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Burkholderia ambifaria (strain MC40-6) OX=398577 GN=pdxH PE=3 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.3e-07
Identity = 36/102 (35.29%), Postives = 52/102 (50.98%), Query Frame = 0

Query: 20  AQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYTDSRSRKIEELKTCPFAEIS 79
           A++     + LAT+G NGRPS R V+ +G +E   G   +T+  SRK  +L   P+A + 
Sbjct: 39  AKLPEPNTMTLATVGENGRPSARIVLIKGVDE--RGFVFFTNYESRKGRDLAAHPYAALL 98

Query: 80  WYFTESWEQFRISGRIEVVDASCLD----ATKFKKRAEAWSS 118
           +Y+ E   Q RI GRIE       D    +     R  AW+S
Sbjct: 99  FYWIELERQVRIEGRIEKTSTDESDRYFASRPLGSRIGAWAS 138

BLAST of CmaCh14G021190 vs. ExPASy Swiss-Prot
Match: Q0BCF1 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) OX=339670 GN=pdxH PE=3 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.3e-07
Identity = 36/102 (35.29%), Postives = 52/102 (50.98%), Query Frame = 0

Query: 20  AQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYTDSRSRKIEELKTCPFAEIS 79
           A++     + LAT+G NGRPS R V+ +G +E   G   +T+  SRK  +L   P+A + 
Sbjct: 39  AKLPEPNTMTLATVGENGRPSARIVLIKGVDE--RGFVFFTNYESRKGRDLAAHPYAALL 98

Query: 80  WYFTESWEQFRISGRIEVVDASCLD----ATKFKKRAEAWSS 118
           +Y+ E   Q RI GRIE       D    +     R  AW+S
Sbjct: 99  FYWIELERQVRIEGRIEKTSTDESDRYFASRPLGSRIGAWAS 138

BLAST of CmaCh14G021190 vs. ExPASy TrEMBL
Match: A0A6J1J0G7 (Pyridoxal 5'-phosphate synthase OS=Cucurbita maxima OX=3661 GN=LOC111480118 PE=4 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 2.4e-110
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0

Query: 1   MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT 60
           MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT
Sbjct: 1   MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT 60

Query: 61  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 120
           DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP
Sbjct: 61  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 120

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 180
           KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK
Sbjct: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 180

Query: 181 AIPCLGRETLWESERINP 199
           AIPCLGRETLWESERINP
Sbjct: 181 AIPCLGRETLWESERINP 198

BLAST of CmaCh14G021190 vs. ExPASy TrEMBL
Match: A0A6J1EFU0 (Pyridoxal 5'-phosphate synthase OS=Cucurbita moschata OX=3662 GN=LOC111432127 PE=4 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 2.1e-106
Identity = 192/198 (96.97%), Postives = 193/198 (97.47%), Query Frame = 0

Query: 1   MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT 60
           MGSAAAPWKSLLLSALESNAQI HS FLHLATIG NGRPSNRTVVFRGFEEGTD I IYT
Sbjct: 24  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 83

Query: 61  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 120
           DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP
Sbjct: 84  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 143

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 180
           KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK
Sbjct: 144 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 203

Query: 181 AIPCLGRETLWESERINP 199
           AIPC+GRETLWESERINP
Sbjct: 204 AIPCVGRETLWESERINP 221

BLAST of CmaCh14G021190 vs. ExPASy TrEMBL
Match: A0A5A7U3K2 (Pyridoxal 5'-phosphate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G00920 PE=4 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 5.0e-92
Identity = 168/198 (84.85%), Postives = 177/198 (89.39%), Query Frame = 0

Query: 1   MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT 60
           MGSA APWKSLLL ALESN QI HS+FL LATIG NGRPSNRTVVFR FEEGTD I IYT
Sbjct: 1   MGSAVAPWKSLLLRALESNGQIKHSKFLQLATIGTNGRPSNRTVVFREFEEGTDRIHIYT 60

Query: 61  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 120
           DSRSRKIEELK CPFAEI WYFT++WEQFRISGR+EVVDASCLDATKFK+RAEAWSSISP
Sbjct: 61  DSRSRKIEELKNCPFAEICWYFTDTWEQFRISGRMEVVDASCLDATKFKQRAEAWSSISP 120

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 180
           KSRMQYLGPSP LP++AEQPAKEP LDS SGPVDAFCLLV DPDQVDYLNLKS ER LF+
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSYSGPVDAFCLLVFDPDQVDYLNLKSEERNLFR 180

Query: 181 AIPCLGRETLWESERINP 199
           AIP  G E LWE+ERINP
Sbjct: 181 AIPSFGGEKLWETERINP 198

BLAST of CmaCh14G021190 vs. ExPASy TrEMBL
Match: A0A1S4DSQ7 (Pyridoxal 5'-phosphate synthase OS=Cucumis melo OX=3656 GN=LOC103483173 PE=4 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 6.6e-92
Identity = 167/198 (84.34%), Postives = 177/198 (89.39%), Query Frame = 0

Query: 1   MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT 60
           MGSA APWKSLLL ALESN QI HS+FL LATIG NGRPSNRTVVFR FEEGTD I IYT
Sbjct: 1   MGSAVAPWKSLLLRALESNGQIKHSKFLQLATIGTNGRPSNRTVVFREFEEGTDRIHIYT 60

Query: 61  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 120
           DSRSRKIEELK CPFAE+ WYFT++WEQFRISGR+EVVDASCLDATKFK+RAEAWSSISP
Sbjct: 61  DSRSRKIEELKNCPFAEVCWYFTDTWEQFRISGRMEVVDASCLDATKFKQRAEAWSSISP 120

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 180
           KSRMQYLGPSP LP++AEQPAKEP LDS SGPVDAFCLLV DPDQVDYLNLKS ER LF+
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSYSGPVDAFCLLVFDPDQVDYLNLKSEERNLFR 180

Query: 181 AIPCLGRETLWESERINP 199
           AIP  G E LWE+ERINP
Sbjct: 181 AIPSFGGEKLWETERINP 198

BLAST of CmaCh14G021190 vs. ExPASy TrEMBL
Match: A0A5D3DBA0 (Pyridoxal 5'-phosphate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G00900 PE=4 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 6.6e-92
Identity = 167/198 (84.34%), Postives = 177/198 (89.39%), Query Frame = 0

Query: 1   MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT 60
           MGSA APWKSLLL ALESN QI HS+FL LATIG NGRPSNRTVVFR FEEGTD I IYT
Sbjct: 1   MGSAVAPWKSLLLRALESNGQIKHSKFLQLATIGTNGRPSNRTVVFREFEEGTDRIHIYT 60

Query: 61  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 120
           DSRSRKIEELK CPFAE+ WYFT++WEQFRISGR+EVVDASCLDATKFK+RAEAWSSISP
Sbjct: 61  DSRSRKIEELKNCPFAEVCWYFTDTWEQFRISGRMEVVDASCLDATKFKQRAEAWSSISP 120

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 180
           KSRMQYLGPSP LP++AEQPAKEP LDS SGPVDAFCLLV DPDQVDYLNLKS ER LF+
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSYSGPVDAFCLLVFDPDQVDYLNLKSEERNLFR 180

Query: 181 AIPCLGRETLWESERINP 199
           AIP  G E LWE+ERINP
Sbjct: 181 AIPSFGGEKLWETERINP 198

BLAST of CmaCh14G021190 vs. NCBI nr
Match: XP_022980848.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 407.9 bits (1047), Expect = 4.9e-110
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0

Query: 1   MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT 60
           MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT
Sbjct: 1   MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT 60

Query: 61  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 120
           DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP
Sbjct: 61  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 120

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 180
           KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK
Sbjct: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 180

Query: 181 AIPCLGRETLWESERINP 199
           AIPCLGRETLWESERINP
Sbjct: 181 AIPCLGRETLWESERINP 198

BLAST of CmaCh14G021190 vs. NCBI nr
Match: KAG7019079.1 (Pyridoxine/pyridoxamine 5'-phosphate oxidase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 394.8 bits (1013), Expect = 4.3e-106
Identity = 192/198 (96.97%), Postives = 193/198 (97.47%), Query Frame = 0

Query: 1   MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT 60
           MGSAAAPWKSLLLSALESNAQI HS FLHLATIG NGRPSNRTVVFRGFEEGTD I IYT
Sbjct: 18  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 77

Query: 61  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 120
           DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP
Sbjct: 78  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 137

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 180
           KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK
Sbjct: 138 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 197

Query: 181 AIPCLGRETLWESERINP 199
           AIPC+GRETLWESERINP
Sbjct: 198 AIPCVGRETLWESERINP 215

BLAST of CmaCh14G021190 vs. NCBI nr
Match: XP_022924710.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita moschata] >KAG6582681.1 Pyridoxine/pyridoxamine 5'-phosphate oxidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 394.8 bits (1013), Expect = 4.3e-106
Identity = 192/198 (96.97%), Postives = 193/198 (97.47%), Query Frame = 0

Query: 1   MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT 60
           MGSAAAPWKSLLLSALESNAQI HS FLHLATIG NGRPSNRTVVFRGFEEGTD I IYT
Sbjct: 24  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 83

Query: 61  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 120
           DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP
Sbjct: 84  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 143

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 180
           KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK
Sbjct: 144 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 203

Query: 181 AIPCLGRETLWESERINP 199
           AIPC+GRETLWESERINP
Sbjct: 204 AIPCVGRETLWESERINP 221

BLAST of CmaCh14G021190 vs. NCBI nr
Match: XP_023527386.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023527387.1 pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 394.0 bits (1011), Expect = 7.4e-106
Identity = 192/198 (96.97%), Postives = 192/198 (96.97%), Query Frame = 0

Query: 1   MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT 60
           MGSAAAPWKSLLLSALESNAQI HSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGI IYT
Sbjct: 1   MGSAAAPWKSLLLSALESNAQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYT 60

Query: 61  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 120
           DSRSRKIEELK CPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSIS 
Sbjct: 61  DSRSRKIEELKACPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISL 120

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 180
           KSRMQYLGPSPNLPYIAEQPAKEPVLD CSGPVDAFCLLVLDPDQVDYLNLKS ERKLFK
Sbjct: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDPCSGPVDAFCLLVLDPDQVDYLNLKSEERKLFK 180

Query: 181 AIPCLGRETLWESERINP 199
           AIPCLGRETLWESERINP
Sbjct: 181 AIPCLGRETLWESERINP 198

BLAST of CmaCh14G021190 vs. NCBI nr
Match: KAA0048877.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 347.1 bits (889), Expect = 1.0e-91
Identity = 168/198 (84.85%), Postives = 177/198 (89.39%), Query Frame = 0

Query: 1   MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT 60
           MGSA APWKSLLL ALESN QI HS+FL LATIG NGRPSNRTVVFR FEEGTD I IYT
Sbjct: 1   MGSAVAPWKSLLLRALESNGQIKHSKFLQLATIGTNGRPSNRTVVFREFEEGTDRIHIYT 60

Query: 61  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 120
           DSRSRKIEELK CPFAEI WYFT++WEQFRISGR+EVVDASCLDATKFK+RAEAWSSISP
Sbjct: 61  DSRSRKIEELKNCPFAEICWYFTDTWEQFRISGRMEVVDASCLDATKFKQRAEAWSSISP 120

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 180
           KSRMQYLGPSP LP++AEQPAKEP LDS SGPVDAFCLLV DPDQVDYLNLKS ER LF+
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSYSGPVDAFCLLVFDPDQVDYLNLKSEERNLFR 180

Query: 181 AIPCLGRETLWESERINP 199
           AIP  G E LWE+ERINP
Sbjct: 181 AIPSFGGEKLWETERINP 198

BLAST of CmaCh14G021190 vs. TAIR 10
Match: AT2G46580.1 (Pyridoxamine 5'-phosphate oxidase family protein )

HSP 1 Score: 234.6 bits (597), Expect = 7.0e-62
Identity = 110/198 (55.56%), Postives = 150/198 (75.76%), Query Frame = 0

Query: 1   MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT 60
           MG+  APWK LL  A+E+N+ ++HS ++ LATIG NGRPSNRTVVFRGFEE +D I+I T
Sbjct: 1   MGTHVAPWKQLLFGAIEANSHLSHSSYVQLATIGLNGRPSNRTVVFRGFEENSDRIQINT 60

Query: 61  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 120
           D RSRKIEELK CPF+E+ WYF+++WEQFRI+GRIEV+DAS  D TK ++R +AW + S 
Sbjct: 61  DLRSRKIEELKHCPFSEMCWYFSDTWEQFRINGRIEVIDASNPDQTKLQQREKAWFANSL 120

Query: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 180
           +SR+ Y+ P+P  P  +EQ +++  LD  SGPV  +CLL+L+P++VDYLNLK+ +R  F 
Sbjct: 121 RSRLIYVCPTPGSPCNSEQSSQQVKLDPSSGPVPEYCLLLLEPEKVDYLNLKTNQRLFFS 180

Query: 181 AIPCLGRETLWESERINP 199
           ++     E  W SE++NP
Sbjct: 181 SMATGTGEKCWTSEKVNP 198

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZPY19.8e-6155.56Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 OS=Arabidopsis thaliana OX=3702 G... [more]
Q7P2034.3e-0833.33Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Chromobacterium violaceum (strai... [more]
A1TQY31.3e-0736.27Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Acidovorax citrulli (strain AAC0... [more]
B1YVG11.3e-0735.29Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Burkholderia ambifaria (strain M... [more]
Q0BCF11.3e-0735.29Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Burkholderia ambifaria (strain A... [more]
Match NameE-valueIdentityDescription
A0A6J1J0G72.4e-110100.00Pyridoxal 5'-phosphate synthase OS=Cucurbita maxima OX=3661 GN=LOC111480118 PE=4... [more]
A0A6J1EFU02.1e-10696.97Pyridoxal 5'-phosphate synthase OS=Cucurbita moschata OX=3662 GN=LOC111432127 PE... [more]
A0A5A7U3K25.0e-9284.85Pyridoxal 5'-phosphate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S4DSQ76.6e-9284.34Pyridoxal 5'-phosphate synthase OS=Cucumis melo OX=3656 GN=LOC103483173 PE=4 SV=... [more]
A0A5D3DBA06.6e-9284.34Pyridoxal 5'-phosphate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
Match NameE-valueIdentityDescription
XP_022980848.14.9e-110100.00pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita maxima][more]
KAG7019079.14.3e-10696.97Pyridoxine/pyridoxamine 5'-phosphate oxidase 2, partial [Cucurbita argyrosperma ... [more]
XP_022924710.14.3e-10696.97pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita moschata] >... [more]
XP_023527386.17.4e-10696.97pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita pepo subsp.... [more]
KAA0048877.11.0e-9184.85pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT2G46580.17.0e-6255.56Pyridoxamine 5'-phosphate oxidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024015Pyridoxamine 5'-phosphate oxidase, probable FMN-dependent, Alr4036 familyTIGRFAMTIGR04026TIGR04026coord: 6..198
e-value: 6.6E-48
score: 161.1
IPR024624Pyridoxamine 5'-phosphate oxidase, Alr4036 family, FMN-binding domainPFAMPF12766Pyridox_oxase_2coord: 6..98
e-value: 8.6E-26
score: 90.5
IPR012349FMN-binding split barrelGENE3D2.30.110.10coord: 3..198
e-value: 5.3E-54
score: 185.0
NoneNo IPR availablePIRSRPIRSR000190-2PIRSR000190-2coord: 24..114
e-value: 1.2E-8
score: 32.7
NoneNo IPR availablePANTHERPTHR10851:SF3PYRIDOXINE/PYRIDOXAMINE 5'-PHOSPHATE OXIDASE 2coord: 2..198
NoneNo IPR availableSUPERFAMILY50475FMN-binding split barrelcoord: 3..198
IPR000659Pyridoxamine 5'-phosphate oxidasePANTHERPTHR10851PYRIDOXINE-5-PHOSPHATE OXIDASEcoord: 2..198

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh14G021190.1CmaCh14G021190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042823 pyridoxal phosphate biosynthetic process
biological_process GO:0008615 pyridoxine biosynthetic process
molecular_function GO:0010181 FMN binding
molecular_function GO:0004733 pyridoxamine-phosphate oxidase activity