CmaCh14G019510 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh14G019510
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	RING-type E3 ubiquitin transferase
Location	Cma_Chr14: 13737076 .. 13738359 (+)
RNA-Seq Expression	CmaCh14G019510
Synteny	CmaCh14G019510

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCGTTTTGCAGATACAAACCTTCGATCATCAATGGATTCCGATTTTCCTCCTCTTTTCCGATGCCCCATTTCCATGGAGCTCATGGAAGATCCCGTTACGATCTCCACCGGCGTCACCTACGAGAGAAAGAGCATCGAAAAATGGCTCTTCACTTACAACAAAAGAACGTGTCCGGCGACGATGCAGACCGTCGCCGTCGGCGAGAACGACTTCGTCATGACTCCTAATCTCACGCTCAAACGCGTCATACTCGCTTGGAAAATCAACGAGTCTTCCTCGTCGCCGGCGACGGCGATCGATCAAGTCCAATCGCTTCTTGCCACTGTTGAGTCTTCTCCGTTTAAGGTGAGTTCGTTGAGGAAATTGCGATCAATGATCGAAATGAGGGATGAGGTGAAATCGGAATTTATTCGATTGAATGGGATAGGGATTGTTGTAAATGTTGTGGTTCAAATTCTCATTGATTGTTCTGATTTCTCGACGTTGGAGGCCTGTGAGGAGGCTTTGGGAGTTCTGTCTCAGTTTCCAATTTCAAATGAAGACGAACTGTTCGACGAAATGTCTAAGAGAGAAGAATTGATGAAATCCGTAGCTATTGTTCTACAAAGGGGGAGTGCAGAAGGGCGTTTTTACGCCGTGAAAATCCTCAGAAATATTGCCAAAAACACCAATTACAATTGGGGATTTGTCATGGAAGAACAGGGCATTGATCTCTTCAAGCCATTGTTGGAGCTTGTATCAGACGAATTTCCCACCAAGTCAAGCTCGTGCGCATTGGATGTAATGATAGAAATTCTAGCGTCATCGAAGAGGAGCCGAGTAAAGGCGATCGAGGCTGGTGCCGTGTGGATCCTGATCGAGCTCTTACCGGAGTCAAGCCGATCGAAATGCGAGAGAATGCTGCAGATTCTGCAGAGTTTATGTGAATGTGCAGAGGGGAGATTGGCTTTGGTGGAACATGGGATGGGAATAGCGGCTGTGACTAAGAAGATTTTGAACGTTTCAAATGTAGCAACAAAAATTGGGGTCAAGATCTTGTTTTGGATTAGTAGTTTTCACCCCAAGGAGAGGGTTGTGGAGGAGATGATGGCTTGTGGGGCAGTGAAGAAGCTGCTGATGATACTTCACATGGCCTGCAGCGGTGTCGGTGGCGGTGGCCGGAGTTCAACAAAGGAGAAGGTGATCAAGATTTTGAAGATGCATGGGAGTTCATGGAGGCGTAGCCCTTGTTTTCCTTGTGAGTTGAAGAATTATTTGAAGCTTGTGAATGATGATTGA

mRNA sequence

ATGGAGCGTTTTGCAGATACAAACCTTCGATCATCAATGGATTCCGATTTTCCTCCTCTTTTCCGATGCCCCATTTCCATGGAGCTCATGGAAGATCCCGTTACGATCTCCACCGGCGTCACCTACGAGAGAAAGAGCATCGAAAAATGGCTCTTCACTTACAACAAAAGAACGTGTCCGGCGACGATGCAGACCGTCGCCGTCGGCGAGAACGACTTCGTCATGACTCCTAATCTCACGCTCAAACGCGTCATACTCGCTTGGAAAATCAACGAGTCTTCCTCGTCGCCGGCGACGGCGATCGATCAAGTCCAATCGCTTCTTGCCACTGTTGAGTCTTCTCCGTTTAAGGTGAGTTCGTTGAGGAAATTGCGATCAATGATCGAAATGAGGGATGAGGTGAAATCGGAATTTATTCGATTGAATGGGATAGGGATTGTTGTAAATGTTGTGGTTCAAATTCTCATTGATTGTTCTGATTTCTCGACGTTGGAGGCCTGTGAGGAGGCTTTGGGAGTTCTGTCTCAGTTTCCAATTTCAAATGAAGACGAACTGTTCGACGAAATGTCTAAGAGAGAAGAATTGATGAAATCCGTAGCTATTGTTCTACAAAGGGGGAGTGCAGAAGGGCGTTTTTACGCCGTGAAAATCCTCAGAAATATTGCCAAAAACACCAATTACAATTGGGGATTTGTCATGGAAGAACAGGGCATTGATCTCTTCAAGCCATTGTTGGAGCTTGTATCAGACGAATTTCCCACCAAGTCAAGCTCGTGCGCATTGGATGTAATGATAGAAATTCTAGCGTCATCGAAGAGGAGCCGAGTAAAGGCGATCGAGGCTGGTGCCGTGTGGATCCTGATCGAGCTCTTACCGGAGTCAAGCCGATCGAAATGCGAGAGAATGCTGCAGATTCTGCAGAGTTTATGTGAATGTGCAGAGGGGAGATTGGCTTTGGTGGAACATGGGATGGGAATAGCGGCTGTGACTAAGAAGATTTTGAACGTTTCAAATGTAGCAACAAAAATTGGGGTCAAGATCTTGTTTTGGATTAGTAGTTTTCACCCCAAGGAGAGGGTTGTGGAGGAGATGATGGCTTGTGGGGCAGTGAAGAAGCTGCTGATGATACTTCACATGGCCTGCAGCGGTGTCGGTGGCGGTGGCCGGAGTTCAACAAAGGAGAAGGTGATCAAGATTTTGAAGATGCATGGGAGTTCATGGAGGCGTAGCCCTTGTTTTCCTTGTGAGTTGAAGAATTATTTGAAGCTTGTGAATGATGATTGA

Coding sequence (CDS)

ATGGAGCGTTTTGCAGATACAAACCTTCGATCATCAATGGATTCCGATTTTCCTCCTCTTTTCCGATGCCCCATTTCCATGGAGCTCATGGAAGATCCCGTTACGATCTCCACCGGCGTCACCTACGAGAGAAAGAGCATCGAAAAATGGCTCTTCACTTACAACAAAAGAACGTGTCCGGCGACGATGCAGACCGTCGCCGTCGGCGAGAACGACTTCGTCATGACTCCTAATCTCACGCTCAAACGCGTCATACTCGCTTGGAAAATCAACGAGTCTTCCTCGTCGCCGGCGACGGCGATCGATCAAGTCCAATCGCTTCTTGCCACTGTTGAGTCTTCTCCGTTTAAGGTGAGTTCGTTGAGGAAATTGCGATCAATGATCGAAATGAGGGATGAGGTGAAATCGGAATTTATTCGATTGAATGGGATAGGGATTGTTGTAAATGTTGTGGTTCAAATTCTCATTGATTGTTCTGATTTCTCGACGTTGGAGGCCTGTGAGGAGGCTTTGGGAGTTCTGTCTCAGTTTCCAATTTCAAATGAAGACGAACTGTTCGACGAAATGTCTAAGAGAGAAGAATTGATGAAATCCGTAGCTATTGTTCTACAAAGGGGGAGTGCAGAAGGGCGTTTTTACGCCGTGAAAATCCTCAGAAATATTGCCAAAAACACCAATTACAATTGGGGATTTGTCATGGAAGAACAGGGCATTGATCTCTTCAAGCCATTGTTGGAGCTTGTATCAGACGAATTTCCCACCAAGTCAAGCTCGTGCGCATTGGATGTAATGATAGAAATTCTAGCGTCATCGAAGAGGAGCCGAGTAAAGGCGATCGAGGCTGGTGCCGTGTGGATCCTGATCGAGCTCTTACCGGAGTCAAGCCGATCGAAATGCGAGAGAATGCTGCAGATTCTGCAGAGTTTATGTGAATGTGCAGAGGGGAGATTGGCTTTGGTGGAACATGGGATGGGAATAGCGGCTGTGACTAAGAAGATTTTGAACGTTTCAAATGTAGCAACAAAAATTGGGGTCAAGATCTTGTTTTGGATTAGTAGTTTTCACCCCAAGGAGAGGGTTGTGGAGGAGATGATGGCTTGTGGGGCAGTGAAGAAGCTGCTGATGATACTTCACATGGCCTGCAGCGGTGTCGGTGGCGGTGGCCGGAGTTCAACAAAGGAGAAGGTGATCAAGATTTTGAAGATGCATGGGAGTTCATGGAGGCGTAGCCCTTGTTTTCCTTGTGAGTTGAAGAATTATTTGAAGCTTGTGAATGATGATTGA

Protein sequence

MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDELFDEMSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDLFKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERVVEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKNYLKLVNDD

Homology

[Close]

BLAST of CmaCh14G019510 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 3.6e-52
Identity = 134/415 (32.29%), Postives = 222/415 (53.49%), Query Frame = 0


Query: 16  DFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVM 75
           + PP F CPIS+E+M+DPV +STG+TY+R SIEKWLF   K +CP T Q +     D  +
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDI----TDADL 70

Query: 76  TPNLTLKRVILAW-KINES-------SSSPATAIDQVQSLLA-TVESSPFKVSSLRKLRS 135
           TPN TL+R+I +W  +N S       +  P     +++ L+  +  S   +V  L++LR 
Sbjct: 71  TPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQ 130

Query: 136 MIEMRDEVKSEFIRLNGI-GIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDEL 195
           ++   +      +   G+   + N+V       +D       +EAL +L     S E  L
Sbjct: 131 IVS-ENATNKRCLEAAGVPEFLANIV------SNDSENGSLTDEALNLLYHLETS-ETVL 190

Query: 196 FDEMSKREE--LMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDLFKP 255
            + ++ +++  ++KS+  ++QRG  E R YA  +L+NI +  +      ++    ++F  
Sbjct: 191 KNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP---EVFTE 250

Query: 256 LLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPE----SSRSKC 315
           +++++ D    K++  A+ +++ I     R+R KA+EAG + ++IELL +    S R   
Sbjct: 251 VVQILDDRISQKATKAAMHILVNICPWG-RNRHKAVEAGVISVIIELLMDESFTSERRGP 310

Query: 316 ERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKER 375
           E  + +L  LC+CAEGR   + HG  IA V KKIL VS  A+   V++L  +  F     
Sbjct: 311 EMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPA 370

Query: 376 VVEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFP 415
           ++ EM+  G V KL ++L ++C G        TKEK  ++LK+H   W+ SPC P
Sbjct: 371 LLHEMLQLGVVAKLCLVLQVSCGG-------KTKEKAKELLKLHARVWKDSPCLP 402

[Close]

BLAST of CmaCh14G019510 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 6.4e-49
Identity = 137/440 (31.14%), Postives = 226/440 (51.36%), Query Frame = 0


Query: 16  DFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVM 75
           + P  F CPIS+++M+DPV +STG+TY+R+SIEKWLF+  K +CP T Q +   E D  +
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVIT--ETD--L 65

Query: 76  TPNLTLKRVILAW-KINES-------SSSPATAIDQVQSLLATVESSPF-KVSSLRKLRS 135
           TPN TL+R+I +W  +N S       +  P     +++ L+    SS   +V  L++LR 
Sbjct: 66  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 125

Query: 136 MIEMRDEVKSEFIRLNGIGIVVNVVVQI---------------LIDCSDFSTLE------ 195
           ++      K           + N+V                  L D    + LE      
Sbjct: 126 IVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSS 185

Query: 196 --ACEEALGVLSQFPISNEDELFDEMSKRE--ELMKSVAIVLQRGSAEGRFYAVKILRNI 255
               +EAL VL     S E  L   ++ ++   L+K++  ++QRG  E R YA  +L+ +
Sbjct: 186 RSLMDEALSVLYHLDTS-ETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 245

Query: 256 AKNTNYNWGFVMEEQGIDLFKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEA 315
            +  +     ++E    +LF  +++++ D+   K++  A+ +++ I     R+R KA+E 
Sbjct: 246 LEVADPMQIILLER---ELFGEVIQILHDQISHKATRSAMQILV-ITCPWGRNRHKAVEG 305

Query: 316 GAVWILIELLPE----SSRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 375
           G + ++IELL +    S R   E  + +L  LC+CAEGR   + HG  IA V+KKIL VS
Sbjct: 306 GTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVS 365

Query: 376 NVATKIGVKILFWISSFHPKERVVEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVI 418
            + ++  V++L  +  F     +++EM+  G V KL ++L ++C        + TKEK  
Sbjct: 366 QITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCG-------NKTKEKAK 425

[Close]

BLAST of CmaCh14G019510 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.3e-33
Identity = 134/455 (29.45%), Postives = 217/455 (47.69%), Query Frame = 0


Query: 16  DFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVM 75
           + P  F CPIS+E+M+DPVT  +G+TY+R++I KWL      +CP T Q + +   D  +
Sbjct: 9   EIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPL---DSDL 68

Query: 76  TPNLTLKRVILAWKI-NESSSSPATAIDQV-QSLLATVESSPFKVSSLRKLRSMIEMRDE 135
           TPN  L+R+I  W + NE+      +  +V    L  VE    ++ +L+K       R+E
Sbjct: 69  TPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVE----EIKNLKKFGQEALGREE 128

Query: 136 V--KSEFIRLNG--------IGIVVNVVVQILIDCSDFS----TLEACEEALGVLSQFPI 195
              K E + ++G         G+  ++++ ++   S+       ++  +E+L +L    I
Sbjct: 129 TLQKLEVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGI 188

Query: 196 SNEDELFDEMSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGID 255
            + D     + + + +M+S+  VL +     + Y + +LRN+   T Y    ++E    +
Sbjct: 189 PSNDAK-TILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNL---TEYTSSHIVERLNPE 248

Query: 256 LFKPLLELVSD-------EFPT-----KSSS-----------------------CALDVM 315
           +FK ++  + D         PT     +SSS                        AL ++
Sbjct: 249 IFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMIL 308

Query: 316 IEILASSKRSRVKAIEAGAVWILIELLPESSRSK--CERMLQILQSLCECAEGRLALVEH 375
           +E  +S  R+R   ++ GAV  LIEL    +  K   E ML +L  LC CA GR  ++ H
Sbjct: 309 LE-TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAH 368

Query: 376 GMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERVVEEMMACGAVKKLLMILHMACS 418
             GIA VTK++L VS  A    + IL  +S F P+  VVEEM+  G V+KL  +L M C 
Sbjct: 369 RGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCG 428

[Close]

BLAST of CmaCh14G019510 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 3.5e-31
Identity = 122/411 (29.68%), Postives = 193/411 (46.96%), Query Frame = 0


Query: 18  PPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVMTP 77
           P  FRCPIS+ELM+DPVT+ TG TY+R SIE W+   N  TCP T   +    +DF + P
Sbjct: 15  PYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPL----SDFTLIP 74

Query: 78  NLTLKRVILAWKINESS---------------SSPATAIDQVQSLLATVESSPFKVSSLR 137
           N TL+R+I  W +   S               +S    + Q  ++  T  S   + ++LR
Sbjct: 75  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALR 134

Query: 138 KLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNE 197
           +LR      D+ +      N   I++ ++       S+ ++ E   E+L +L   PI+ E
Sbjct: 135 RLRGFARDSDKNRVLIAAHNATEILIKILF------SETTSSELVSESLALLVMLPIT-E 194

Query: 198 DELFDEMSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYN--WGFVMEEQGIDL 257
              F  +S     ++ +  +L   S E R  A  ++  ++  T      G +   + +  
Sbjct: 195 PNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESV-- 254

Query: 258 FKPLLELVSDEFPTKSSSCALDVMIE---ILASSKRSRVKAIEAGAVWILIE-LLPESSR 317
           F+ +L+L+ +     SS  AL + I+    L S K +R  AI AGA  ILI+ L  +  R
Sbjct: 255 FEGVLDLLRNPI---SSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDR 314

Query: 318 SKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHP 377
              ER L  ++ LC   EG  A  EH + +  + K IL VS+ AT+     L  + +   
Sbjct: 315 CDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCT--A 374

Query: 378 KERVVEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSW 408
           +ER  EE    G V +LL+++   C+          K+K  K+LK+   SW
Sbjct: 375 EERWREEAAGAGVVVQLLLMVQSECT-------ERAKKKAQKLLKLLRDSW 400

[Close]

BLAST of CmaCh14G019510 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 4.5e-26
Identity = 115/405 (28.40%), Postives = 194/405 (47.90%), Query Frame = 0


Query: 18  PPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVMTP 77
           PP F+CPIS++LM+DPV ISTG+TY+R SIE W+ + NK TCP T   +      F   P
Sbjct: 32  PPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNK-TCPVTNTVLTT----FDQIP 91

Query: 78  NLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSL-RKLRSMIEMRDEVKS 137
           N T++++I  W + + S      I ++ +    V   P +V  + RKL S     D  K 
Sbjct: 92  NHTIRKMIQGWCVEKGS----PLIQRIPT--PRVPLMPCEVYEISRKLSSATRRGDYEKC 151

Query: 138 EFI--RLNGIG---------IVVNVVVQILIDCSD-FSTLE----ACEEALGVLS-QFPI 197
             I  ++  +G         +  N V  +L DC D FS  E       E L +L+  FPI
Sbjct: 152 GVIIEKIKKLGDESEKNRKCVNENSVGWVLCDCFDKFSGDEKLTFMLNEILSLLTWMFPI 211

Query: 198 SNEDELFDEMSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGID 257
             E     +++      + VA +L+      R  A  I++ I             E G+ 
Sbjct: 212 GLEG--ISKLASATS-FRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGV- 271

Query: 258 LFKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKC 317
             + L++L+ D   + S+  +L  + +++        + +E G V I +E++ ++  S C
Sbjct: 272 -AEALVKLIRDSVSSSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVDAENSVC 331

Query: 318 ERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKIL--FWISSFHPK 377
           E+ L +L ++CE   GR  + ++ + +  + KKI  VS +AT+  + ++   W +     
Sbjct: 332 EKALAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTG---N 391

Query: 378 ERVVEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVIKILKM 403
              VE+ +  GA +K+L++L +       G    TKEK  ++LKM
Sbjct: 392 TVAVEDAVRLGAFQKVLLVLQV-------GYGEETKEKATELLKM 410

[Close]

BLAST of CmaCh14G019510 vs. ExPASy TrEMBL
Match: A0A6J1IYW3 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111479714 PE=4 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 3.8e-238
Identity = 427/427 (100.00%), Postives = 427/427 (100.00%), Query Frame = 0


Query: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60
           MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP
Sbjct: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60

Query: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120
           ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS
Sbjct: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120

Query: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180
           LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS
Sbjct: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180

Query: 181 NEDELFDEMSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDL 240
           NEDELFDEMSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDL
Sbjct: 181 NEDELFDEMSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDL 240

Query: 241 FKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCE 300
           FKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCE
Sbjct: 241 FKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCE 300

Query: 301 RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERV 360
           RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERV
Sbjct: 301 RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERV 360

Query: 361 VEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKNY 420
           VEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKNY
Sbjct: 361 VEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKNY 420

Query: 421 LKLVNDD 428
           LKLVNDD
Sbjct: 421 LKLVNDD 427

[Close]

BLAST of CmaCh14G019510 vs. ExPASy TrEMBL
Match: A0A6J1E9J6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432079 PE=4 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 2.8e-233
Identity = 420/427 (98.36%), Postives = 421/427 (98.59%), Query Frame = 0


Query: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60
           MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP
Sbjct: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60

Query: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120
           ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS
Sbjct: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120

Query: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180
           LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS
Sbjct: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180

Query: 181 NEDELFDEMSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDL 240
           NED+LFDEMSKREE MKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDL
Sbjct: 181 NEDKLFDEMSKREEWMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDL 240

Query: 241 FKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCE 300
           FKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCE
Sbjct: 241 FKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCE 300

Query: 301 RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERV 360
           RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKIL WISSFHPKERV
Sbjct: 301 RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILLWISSFHPKERV 360

Query: 361 VEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKNY 420
           VEEMMACGAVKKLLMILHMA  GVGGGGRSSTKEKVIKILKMHGSSWRR PCFPCELK Y
Sbjct: 361 VEEMMACGAVKKLLMILHMASGGVGGGGRSSTKEKVIKILKMHGSSWRRGPCFPCELKYY 420

Query: 421 LKLVNDD 428
           LKLVNDD
Sbjct: 421 LKLVNDD 427

[Close]

BLAST of CmaCh14G019510 vs. ExPASy TrEMBL
Match: A0A0A0L6J2 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G127120 PE=4 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 7.8e-207
Identity = 364/415 (87.71%), Postives = 393/415 (94.70%), Query Frame = 0


Query: 13  MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGEND 72
           MDSDFPPLFRCPISMELMEDPVT+STGVTY+RKSIEKWLFTYNK+TCPATMQT+A G  D
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60

Query: 73  FVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRD 132
           FV+TPNL LKR+ILAWKIN+S+ +PATA+++V SLLATVESSPFKVSSLRKLRSM+EM D
Sbjct: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120

Query: 133 EVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDELFDEMSKR 192
            +KSEFIRL+GIGIVVNVV+QILIDCSDFSTL+ CEEALGVL QFPISNED+LFDEM KR
Sbjct: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQFPISNEDKLFDEMYKR 180

Query: 193 EELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDLFKPLLELVSDEF 252
           EELMKS+A+V+QRGSAEGRFYAVKILRNIAKNTNYNWG +MEEQGID FKPLLELVSDEF
Sbjct: 181 EELMKSIAVVVQRGSAEGRFYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEF 240

Query: 253 PTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCERMLQILQSLCEC 312
           PTK+SSCALDVMIEIL+SSKRSRVKAIEAGAVWILIELLPES RSKCERMLQILQSLCEC
Sbjct: 241 PTKASSCALDVMIEILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 313 AEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERVVEEMMACGAVKK 372
           AEGRLALVEHGMGIAAVTKKILNVS VATKIGVKIL WISSFHP+ERVV+EMMACGAVKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360

Query: 373 LLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKNYLKLVNDD 428
           LLMILHM  +GVG GGRSSTKEKVIKILKMHG+ WRRSPCFP ELKNYLK VND+
Sbjct: 361 LLMILHMDSTGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

[Close]

BLAST of CmaCh14G019510 vs. ExPASy TrEMBL
Match: A0A5D3D2G2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003490 PE=4 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 1.0e-206
Identity = 366/415 (88.19%), Postives = 392/415 (94.46%), Query Frame = 0


Query: 13  MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGEND 72
           MDSDFPPLFRCPISMELMEDPVT+STGVTY+RKSIEKWLFTYNK+TCPATMQT+A G +D
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60

Query: 73  FVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRD 132
           FV+TPNLTLKR+ILAWKIN+S+S+PATA+++V SLLATVESSPFKVSSLRKLRSMIEM D
Sbjct: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSMIEMND 120

Query: 133 EVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDELFDEMSKR 192
            +KSEF+RL+GIGIVVNVV+QILIDCSDFSTLE CEEALGVL QFPISNED+LFDEM KR
Sbjct: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180

Query: 193 EELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDLFKPLLELVSDEF 252
           EELMKS+ +VLQRGSAEGRFYAVKILRNIAKNTNYNWG VMEEQ ID FKPLLELVSDEF
Sbjct: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240

Query: 253 PTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCERMLQILQSLCEC 312
           PTK+SSCALDVMI IL+SSKRSRVKAIEAGAVWILIELLPES RSKCERMLQILQSLCEC
Sbjct: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 313 AEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERVVEEMMACGAVKK 372
           AEGRLALVEHGMGIAAVTKKILNVS VATKIGVKIL WISSFHP+ERVV+EMMACGAVKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360

Query: 373 LLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKNYLKLVNDD 428
           LLMILHM   GVG GGRSSTKEKVIKILKMHG+ WRRSPCFP ELKNYLK VND+
Sbjct: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

[Close]

BLAST of CmaCh14G019510 vs. ExPASy TrEMBL
Match: A0A1S3AWJ2 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103483401 PE=4 SV=1)

HSP 1 Score: 727.6 bits (1877), Expect = 2.9e-206
Identity = 365/415 (87.95%), Postives = 392/415 (94.46%), Query Frame = 0


Query: 13  MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGEND 72
           MDSDFPPLFRCPISMELMEDPVT+STGVTY+RKSIEKWLFTYNK+TCPATMQT+A G +D
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60

Query: 73  FVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRD 132
           FV+TPNLTLKR+ILAWKIN+S+S+PATA+++V SLLATVESSPFKVSSLRKLRS+IEM D
Sbjct: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND 120

Query: 133 EVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDELFDEMSKR 192
            +KSEF+RL+GIGIVVNVV+QILIDCSDFSTLE CEEALGVL QFPISNED+LFDEM KR
Sbjct: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180

Query: 193 EELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDLFKPLLELVSDEF 252
           EELMKS+ +VLQRGSAEGRFYAVKILRNIAKNTNYNWG VMEEQ ID FKPLLELVSDEF
Sbjct: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240

Query: 253 PTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCERMLQILQSLCEC 312
           PTK+SSCALDVMI IL+SSKRSRVKAIEAGAVWILIELLPES RSKCERMLQILQSLCEC
Sbjct: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 313 AEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERVVEEMMACGAVKK 372
           AEGRLALVEHGMGIAAVTKKILNVS VATKIGVKIL WISSFHP+ERVV+EMMACGAVKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360

Query: 373 LLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKNYLKLVNDD 428
           LLMILHM   GVG GGRSSTKEKVIKILKMHG+ WRRSPCFP ELKNYLK VND+
Sbjct: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

[Close]

BLAST of CmaCh14G019510 vs. NCBI nr
Match: XP_022980298.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima])

HSP 1 Score: 833.6 bits (2152), Expect = 7.9e-238
Identity = 427/427 (100.00%), Postives = 427/427 (100.00%), Query Frame = 0


Query: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60
           MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP
Sbjct: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60

Query: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120
           ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS
Sbjct: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120

Query: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180
           LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS
Sbjct: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180

Query: 181 NEDELFDEMSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDL 240
           NEDELFDEMSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDL
Sbjct: 181 NEDELFDEMSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDL 240

Query: 241 FKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCE 300
           FKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCE
Sbjct: 241 FKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCE 300

Query: 301 RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERV 360
           RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERV
Sbjct: 301 RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERV 360

Query: 361 VEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKNY 420
           VEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKNY
Sbjct: 361 VEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKNY 420

Query: 421 LKLVNDD 428
           LKLVNDD
Sbjct: 421 LKLVNDD 427

[Close]

BLAST of CmaCh14G019510 vs. NCBI nr
Match: XP_023528267.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 820.5 bits (2118), Expect = 6.9e-234
Identity = 422/427 (98.83%), Postives = 423/427 (99.06%), Query Frame = 0


Query: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60
           MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP
Sbjct: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60

Query: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120
           ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS
Sbjct: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120

Query: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180
           LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS
Sbjct: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180

Query: 181 NEDELFDEMSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDL 240
           NED+LFDEMSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDL
Sbjct: 181 NEDKLFDEMSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDL 240

Query: 241 FKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCE 300
           FKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCE
Sbjct: 241 FKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCE 300

Query: 301 RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERV 360
           RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKIL WISSFHPKERV
Sbjct: 301 RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILLWISSFHPKERV 360

Query: 361 VEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKNY 420
           VEEMMACGAVKKLLMILHMA  GVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELK Y
Sbjct: 361 VEEMMACGAVKKLLMILHMAGGGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKYY 420

Query: 421 LKLVNDD 428
           LKLVNDD
Sbjct: 421 LKLVNDD 427

[Close]

BLAST of CmaCh14G019510 vs. NCBI nr
Match: XP_022924647.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata])

HSP 1 Score: 817.4 bits (2110), Expect = 5.8e-233
Identity = 420/427 (98.36%), Postives = 421/427 (98.59%), Query Frame = 0


Query: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60
           MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP
Sbjct: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60

Query: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120
           ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS
Sbjct: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120

Query: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180
           LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS
Sbjct: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180

Query: 181 NEDELFDEMSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDL 240
           NED+LFDEMSKREE MKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDL
Sbjct: 181 NEDKLFDEMSKREEWMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDL 240

Query: 241 FKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCE 300
           FKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCE
Sbjct: 241 FKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCE 300

Query: 301 RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERV 360
           RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKIL WISSFHPKERV
Sbjct: 301 RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILLWISSFHPKERV 360

Query: 361 VEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKNY 420
           VEEMMACGAVKKLLMILHMA  GVGGGGRSSTKEKVIKILKMHGSSWRR PCFPCELK Y
Sbjct: 361 VEEMMACGAVKKLLMILHMASGGVGGGGRSSTKEKVIKILKMHGSSWRRGPCFPCELKYY 420

Query: 421 LKLVNDD 428
           LKLVNDD
Sbjct: 421 LKLVNDD 427

[Close]

BLAST of CmaCh14G019510 vs. NCBI nr
Match: KAG7018915.1 (E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 813.9 bits (2101), Expect = 6.5e-232
Identity = 417/427 (97.66%), Postives = 421/427 (98.59%), Query Frame = 0


Query: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60
           MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP
Sbjct: 1   MERFADTNLRSSMDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCP 60

Query: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120
           ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS
Sbjct: 61  ATMQTVAVGENDFVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSS 120

Query: 121 LRKLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPIS 180
           LRKLRSM+EMRDEVKSEFIRLNGIGIVVNVV+QILIDCSDFSTLEACEEALGVLSQFPIS
Sbjct: 121 LRKLRSMMEMRDEVKSEFIRLNGIGIVVNVVLQILIDCSDFSTLEACEEALGVLSQFPIS 180

Query: 181 NEDELFDEMSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDL 240
           NED+LFDEMSKREE MKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDL
Sbjct: 181 NEDKLFDEMSKREEWMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDL 240

Query: 241 FKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCE 300
           FKPLLELVSDEFPTKSSSCALDVM+EILASSKRSRVKAIEAGAVWILIELLPESSRSKCE
Sbjct: 241 FKPLLELVSDEFPTKSSSCALDVMMEILASSKRSRVKAIEAGAVWILIELLPESSRSKCE 300

Query: 301 RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERV 360
           RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKIL WISSFHPKERV
Sbjct: 301 RMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILLWISSFHPKERV 360

Query: 361 VEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKNY 420
           VEEMMACGAVKKLLMILHMA  GVGGGGRSSTKEKVIKILKMHGSSWRR PCFPCELK Y
Sbjct: 361 VEEMMACGAVKKLLMILHMASGGVGGGGRSSTKEKVIKILKMHGSSWRRGPCFPCELKYY 420

Query: 421 LKLVNDD 428
           LKLVNDD
Sbjct: 421 LKLVNDD 427

[Close]

BLAST of CmaCh14G019510 vs. NCBI nr
Match: XP_038902365.1 (E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida])

HSP 1 Score: 739.2 bits (1907), Expect = 2.0e-209
Identity = 374/415 (90.12%), Postives = 396/415 (95.42%), Query Frame = 0


Query: 13  MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGEND 72
           M+SDFPPLFRCPISMELMEDPVT+STGVTY+RKSIEKWLFTYNK+TCPATMQT++ G+ D
Sbjct: 1   MESDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLSAGDED 60

Query: 73  FVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRD 132
           FV+TPNLTLKR+ILAWKI +SSSSPATA+++V+SLLATVESSPFKVSSLRKLRSMIEM D
Sbjct: 61  FVITPNLTLKRLILAWKITDSSSSPATALEEVRSLLATVESSPFKVSSLRKLRSMIEMSD 120

Query: 133 EVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDELFDEMSKR 192
            +KSEFIR NGIGIVVNVV+QILIDCSDFSTLE CEEALGVL QFPIS+ED+LFDEMSKR
Sbjct: 121 GMKSEFIRSNGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISDEDKLFDEMSKR 180

Query: 193 EELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDLFKPLLELVSDEF 252
           EELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWG VMEEQGID FKPLLELVSDEF
Sbjct: 181 EELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQGIDFFKPLLELVSDEF 240

Query: 253 PTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCERMLQILQSLCEC 312
            TK+SSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPES RSKCERMLQILQSLCEC
Sbjct: 241 ATKASSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 313 AEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERVVEEMMACGAVKK 372
           AEGRLALVEHGMGIAAVTKKILNVSNVATK+GVKIL WISSFHP+ERVVEEMMACGAVKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPRERVVEEMMACGAVKK 360

Query: 373 LLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKNYLKLVNDD 428
           LLMILHM   GVG GGRSSTKEKVIKILKMHG+SWRRSPCFP ELKNYLK VNDD
Sbjct: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNSWRRSPCFPSELKNYLKFVNDD 415

[Close]

BLAST of CmaCh14G019510 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 207.2 bits (526), Expect = 2.6e-53
Identity = 134/415 (32.29%), Postives = 222/415 (53.49%), Query Frame = 0


Query: 16  DFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVM 75
           + PP F CPIS+E+M+DPV +STG+TY+R SIEKWLF   K +CP T Q +     D  +
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDI----TDADL 70

Query: 76  TPNLTLKRVILAW-KINES-------SSSPATAIDQVQSLLA-TVESSPFKVSSLRKLRS 135
           TPN TL+R+I +W  +N S       +  P     +++ L+  +  S   +V  L++LR 
Sbjct: 71  TPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQ 130

Query: 136 MIEMRDEVKSEFIRLNGI-GIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDEL 195
           ++   +      +   G+   + N+V       +D       +EAL +L     S E  L
Sbjct: 131 IVS-ENATNKRCLEAAGVPEFLANIV------SNDSENGSLTDEALNLLYHLETS-ETVL 190

Query: 196 FDEMSKREE--LMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDLFKP 255
            + ++ +++  ++KS+  ++QRG  E R YA  +L+NI +  +      ++    ++F  
Sbjct: 191 KNLLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKP---EVFTE 250

Query: 256 LLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPE----SSRSKC 315
           +++++ D    K++  A+ +++ I     R+R KA+EAG + ++IELL +    S R   
Sbjct: 251 VVQILDDRISQKATKAAMHILVNICPWG-RNRHKAVEAGVISVIIELLMDESFTSERRGP 310

Query: 316 ERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKER 375
           E  + +L  LC+CAEGR   + HG  IA V KKIL VS  A+   V++L  +  F     
Sbjct: 311 EMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPA 370

Query: 376 VVEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFP 415
           ++ EM+  G V KL ++L ++C G        TKEK  ++LK+H   W+ SPC P
Sbjct: 371 LLHEMLQLGVVAKLCLVLQVSCGG-------KTKEKAKELLKLHARVWKDSPCLP 402

[Close]

BLAST of CmaCh14G019510 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 196.4 bits (498), Expect = 4.5e-50
Identity = 137/440 (31.14%), Postives = 226/440 (51.36%), Query Frame = 0


Query: 16  DFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVM 75
           + P  F CPIS+++M+DPV +STG+TY+R+SIEKWLF+  K +CP T Q +   E D  +
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVIT--ETD--L 65

Query: 76  TPNLTLKRVILAW-KINES-------SSSPATAIDQVQSLLATVESSPF-KVSSLRKLRS 135
           TPN TL+R+I +W  +N S       +  P     +++ L+    SS   +V  L++LR 
Sbjct: 66  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 125

Query: 136 MIEMRDEVKSEFIRLNGIGIVVNVVVQI---------------LIDCSDFSTLE------ 195
           ++      K           + N+V                  L D    + LE      
Sbjct: 126 IVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSS 185

Query: 196 --ACEEALGVLSQFPISNEDELFDEMSKRE--ELMKSVAIVLQRGSAEGRFYAVKILRNI 255
               +EAL VL     S E  L   ++ ++   L+K++  ++QRG  E R YA  +L+ +
Sbjct: 186 RSLMDEALSVLYHLDTS-ETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKL 245

Query: 256 AKNTNYNWGFVMEEQGIDLFKPLLELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEA 315
            +  +     ++E    +LF  +++++ D+   K++  A+ +++ I     R+R KA+E 
Sbjct: 246 LEVADPMQIILLER---ELFGEVIQILHDQISHKATRSAMQILV-ITCPWGRNRHKAVEG 305

Query: 316 GAVWILIELLPE----SSRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVS 375
           G + ++IELL +    S R   E  + +L  LC+CAEGR   + HG  IA V+KKIL VS
Sbjct: 306 GTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVS 365

Query: 376 NVATKIGVKILFWISSFHPKERVVEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVI 418
            + ++  V++L  +  F     +++EM+  G V KL ++L ++C        + TKEK  
Sbjct: 366 QITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCG-------NKTKEKAK 425

[Close]

BLAST of CmaCh14G019510 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 145.6 bits (366), Expect = 9.2e-35
Identity = 134/455 (29.45%), Postives = 217/455 (47.69%), Query Frame = 0


Query: 16  DFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVM 75
           + P  F CPIS+E+M+DPVT  +G+TY+R++I KWL      +CP T Q + +   D  +
Sbjct: 23  EIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPL---DSDL 82

Query: 76  TPNLTLKRVILAWKI-NESSSSPATAIDQV-QSLLATVESSPFKVSSLRKLRSMIEMRDE 135
           TPN  L+R+I  W + NE+      +  +V    L  VE    ++ +L+K       R+E
Sbjct: 83  TPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVE----EIKNLKKFGQEALGREE 142

Query: 136 V--KSEFIRLNG--------IGIVVNVVVQILIDCSDFS----TLEACEEALGVLSQFPI 195
              K E + ++G         G+  ++++ ++   S+       ++  +E+L +L    I
Sbjct: 143 TLQKLEVLAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGI 202

Query: 196 SNEDELFDEMSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGID 255
            + D     + + + +M+S+  VL +     + Y + +LRN+   T Y    ++E    +
Sbjct: 203 PSNDAK-TILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNL---TEYTSSHIVERLNPE 262

Query: 256 LFKPLLELVSD-------EFPT-----KSSS-----------------------CALDVM 315
           +FK ++  + D         PT     +SSS                        AL ++
Sbjct: 263 IFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMIL 322

Query: 316 IEILASSKRSRVKAIEAGAVWILIELLPESSRSK--CERMLQILQSLCECAEGRLALVEH 375
           +E  +S  R+R   ++ GAV  LIEL    +  K   E ML +L  LC CA GR  ++ H
Sbjct: 323 LE-TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAH 382

Query: 376 GMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERVVEEMMACGAVKKLLMILHMACS 418
             GIA VTK++L VS  A    + IL  +S F P+  VVEEM+  G V+KL  +L M C 
Sbjct: 383 RGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCG 442

[Close]

BLAST of CmaCh14G019510 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 137.5 bits (345), Expect = 2.5e-32
Identity = 122/411 (29.68%), Postives = 193/411 (46.96%), Query Frame = 0


Query: 18  PPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVMTP 77
           P  FRCPIS+ELM+DPVT+ TG TY+R SIE W+   N  TCP T   +    +DF + P
Sbjct: 15  PYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPL----SDFTLIP 74

Query: 78  NLTLKRVILAWKINESS---------------SSPATAIDQVQSLLATVESSPFKVSSLR 137
           N TL+R+I  W +   S               +S    + Q  ++  T  S   + ++LR
Sbjct: 75  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALR 134

Query: 138 KLRSMIEMRDEVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNE 197
           +LR      D+ +      N   I++ ++       S+ ++ E   E+L +L   PI+ E
Sbjct: 135 RLRGFARDSDKNRVLIAAHNATEILIKILF------SETTSSELVSESLALLVMLPIT-E 194

Query: 198 DELFDEMSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYN--WGFVMEEQGIDL 257
              F  +S     ++ +  +L   S E R  A  ++  ++  T      G +   + +  
Sbjct: 195 PNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESV-- 254

Query: 258 FKPLLELVSDEFPTKSSSCALDVMIE---ILASSKRSRVKAIEAGAVWILIE-LLPESSR 317
           F+ +L+L+ +     SS  AL + I+    L S K +R  AI AGA  ILI+ L  +  R
Sbjct: 255 FEGVLDLLRNPI---SSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDR 314

Query: 318 SKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHP 377
              ER L  ++ LC   EG  A  EH + +  + K IL VS+ AT+     L  + +   
Sbjct: 315 CDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCT--A 374

Query: 378 KERVVEEMMACGAVKKLLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSW 408
           +ER  EE    G V +LL+++   C+          K+K  K+LK+   SW
Sbjct: 375 EERWREEAAGAGVVVQLLLMVQSECT-------ERAKKKAQKLLKLLRDSW 400

[Close]

BLAST of CmaCh14G019510 vs. TAIR 10
Match: AT5G09800.1 (ARM repeat superfamily protein )

HSP 1 Score: 124.8 bits (312), Expect = 1.7e-28
Identity = 112/380 (29.47%), Postives = 192/380 (50.53%), Query Frame = 0


Query: 18  PPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGENDFVMTP 77
           P  F+CPIS+++M+ PV++STGVTY+R SI++WL   N  TCPATMQ +     +FV  P
Sbjct: 12  PCFFKCPISLDVMKSPVSLSTGVTYDRVSIQRWLDDGN-NTCPATMQILQ--NKEFV--P 71

Query: 78  NLTLKRVILAW--KINE--SSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRDE 137
           NLTL R+I  W   IN    S SP +       + A +E    +  +  K+       DE
Sbjct: 72  NLTLHRLIDHWSDSINRRADSESPESDTPTRDEINAAIERFRIENDARSKILRFARESDE 131

Query: 138 VKSEFIRLNGIGIVVNVVVQILIDCSDF--STLEACEEALGVLSQFPISNEDELFDE--- 197
              EF  L G    V ++V ++ D  +F  S L    EA+ +LS        ++FD    
Sbjct: 132 -NREF--LAGKDDFVAMLVDLISDSRNFSDSQLLLVGEAVKILSMI----RRKIFDRRRL 191

Query: 198 ----MSKREELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDLFKPL 257
               ++   + + S  ++++RG+ + +     +L  IA +       ++  +G  L   +
Sbjct: 192 SNLILTNGGDCLTSFFLLIKRGNPKLKIDCSAVLEFIAVDAESK---LIIAKGEGLVTEI 251

Query: 258 LELVSDEFPTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELL--PESSRSKCERM 317
           ++L+S +  +      L ++I I ASSKR ++  I    V  L  LL  P +S S  E+ 
Sbjct: 252 IKLISSDSDSSLIEANLSLLIAI-ASSKRVKLALIREKLVTKLTSLLTDPTTSVSVTEKC 311

Query: 318 LQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERVVE 377
           L++L+++  C EGR  + + G+ +  V  K++ VS  AT+  V +L+ +     +++  +
Sbjct: 312 LKLLEAISSCKEGRSEICD-GVCVETVVNKLMKVSTAATEHAVTVLWSVCYLFKEKKAQD 371

Query: 378 EMMACGAVKKLLMILHMACS 383
            ++    V K+L++L   CS
Sbjct: 372 AVIRINGVTKILLLLQSNCS 374

The following BLAST results are available for this feature:

BLAST of CmaCh14G019510 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84TG3	3.6e-52	32.29	E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ...	[more]
Q9SVC6	6.4e-49	31.14	E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ...	[more]
Q9SF15	1.3e-33	29.45	E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ...	[more]
Q9LT79	3.5e-31	29.68	U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2...	[more]
Q5PNY6	4.5e-26	28.40	U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2...	[more]

BLAST of CmaCh14G019510 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IYW3	3.8e-238	100.00	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111479714 P...	[more]
A0A6J1E9J6	2.8e-233	98.36	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432079...	[more]
A0A0A0L6J2	7.8e-207	87.71	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G127120 PE...	[more]
A0A5D3D2G2	1.0e-206	88.19	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3AWJ2	2.9e-206	87.95	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103483401 PE=4 ...	[more]

BLAST of CmaCh14G019510 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022980298.1	7.9e-238	100.00	E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima]	[more]
XP_023528267.1	6.9e-234	98.83	E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo]	[more]
XP_022924647.1	5.8e-233	98.36	E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata]	[more]
KAG7018915.1	6.5e-232	97.66	E3 ubiquitin-protein ligase PUB23, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_038902365.1	2.0e-209	90.12	E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida]	[more]

BLAST of CmaCh14G019510 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G35930.1	2.6e-53	32.29	plant U-box 23	[more]
AT3G52450.1	4.5e-50	31.14	plant U-box 22	[more]
AT3G11840.1	9.2e-35	29.45	plant U-box 24	[more]
AT3G19380.1	2.5e-32	29.68	plant U-box 25	[more]
AT5G09800.1	1.7e-28	29.47	ARM repeat superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 20..88 e-value: 1.9E-17 score: 74.0
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 18..90 e-value: 1.2E-14 score: 54.2
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 16..95 score: 18.736959
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 3..105 e-value: 7.4E-22 score: 79.1
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 118..402 e-value: 6.7E-16 score: 59.8
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 17..425
None	No IPR available	PANTHER	PTHR22849:SF23	RING-TYPE E3 UBIQUITIN TRANSFERASE	coord: 17..425
None	No IPR available	CDD	cd16664	RING-Ubox_PUB	coord: 21..64 e-value: 3.08132E-21 score: 84.2074
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 11..91
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 100..378

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh14G019510.1	CmaCh14G019510.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity