CmaCh14G017480 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh14G017480
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptiontransmembrane emp24 domain-containing protein p24beta3-like
LocationCma_Chr14: 12775670 .. 12778292 (+)
RNA-Seq ExpressionCmaCh14G017480
SyntenyCmaCh14G017480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTCCGACCAAAAGTTCTAAACAGAAGAAAACCGAAAGGGTGAGAAAATGGAGAGGAGACCGAGAGAGCGGGGATTCGGATACCTGTTGACGGGTCTTCTTCTGGCGACCTTAATCGCCAGAATCGATTCGCTTTCGGTGACAGTGAACGAGGTTGAATGCATCCATGAATACGTATTGTACGAAGGCGACACCGTTTCTGGGAATTTTGTCGTCGTCGATCATGAAATTTTCTGGGGCGCCGATCATCCTGGCATTGATTTCACTGTAAGCCACATTGTTTCCATCATTAATCTGGTTTTTAATTCATGTTTGCATGACAATGCGTTTCGTTTCTGCCGATTTGTAGATTGATCCCGATTACGATTGGATCTCGGATTGGGGGAATGTGTGGTAGAGTTCGGTTTTTTATTTCCAATTTTTCTGGGCAGTCTTCTTTTCTCTGTTGTGAAATTTGTTTAGGAAAATGTTGTTCGTTGATTTTGACTTATTAAAGAACCTGGATTTTTTTGCCCTTTTGTTAATATGGACGTATGGGCTTGTTTGTTTGTAATCGTGATGATACTTTAGATTGATAATTGTAGTACATTTCTTGTGAGCTTCGAAATGATCTGTTGTTAAACTGTTTATGATCATGAGTTTCACATTTATAATCCTTCGGGCATCCACCTGACACCCATTGACTGTTTTATTTTGCTCGCTTACCAAATGGTGTTGTGAAATTGTTATTTTCTTTGTTCTCACAATTTGATGGGTAATTGGCCGTGCATTTTCCTTTTTTCTTTAATAACTTAGACGAGTCATGTGTTTCACCTCAATTCCTCAGGTCTCTAAAGCGGTGGTAGAAAAAATGTTTAGAAAAGTAGATGAATATGCATGTTTCTTCTTCTTCTTATAACCCATCTGAATCCATAAATGTTATTTCTTTGGTCATCATCGGCTGGATATTAGGGTACCTGGAATCTCTTGAAGGATTTTTGTATCCATAATGAAATGTTGTTTTGAAGATATCCTAACGTGGGTTGGTTTTGTGCAGGTTACATCTCCCGCAGGTAATGTGGTGCACTCGTTGAAGGAGACATCTGGTGGCAAGTTTGAGTTTAAGGCTTCTCGCAGTGGAATGTTTAGATTCTGTTTTCGTAATCCCTACTCGACCACCGAGACAGTTTCTTTCTACATCCATGTTGGTCACATTCCAAACGAACATGACCTTGCTAAAGATGGTATTAATTAACTTCTGCATAGATGCCTACACATTTGTGCTTTGAGGACTTGGTTGCTTTTATGAAGTTGACACTTCTCTCATTTTTCACCTCGTTGTGGTAATTTCTTGCAGAACACCTAGACCCTGTAAATGTTAAAATTGCAGAGCTTAGAGAGGCATTGGAATCGGTCACCGCCGAGCAGAAATATTTGAAAGCACGTGATGCACGCCACCGTCACAGTATGTGGTTTATTTTTGGCATGGTTTATATATATATATAGAAGAAACATATAAATTAATCATAAACGAAGAAATACAGCTTAGGTGCAGGGGGAAGAGAGAACCCCCTCCCAAAGGAATTGGTCGTAGGCATGACACCACCAAGATGTAGGGTCTGTAAACTTATGGCATTTCTTTGAATTCTTGCTAGCGAACGCGATTTCTAACTCTGTGACACAAGATGAATGTCTTTTCTTCTCTAGTGGTCCGACATAACTATCATGATTATGATCTGATCTTGATGTCTATGTGTGAGATTGTAACACACACGATAGCTTAAGCATCATCTTTTAATAATGCATATAGGGATCAAGCGTTGAAACCACGAGCTGAACAATAATTAGCATAGTTTTGAATATTATCCACCAGCATTATATCTCATGAAACTTCGTAGAAACTAGTTTCTTTTTTGTTTCCAGTCTAATATCGATGTAGACAAGAAGTTTATTCCAACCCATCAATTTTTAAGTTGAAATCCAGACTGCTTTGGCAATTGTTCTAGGATTCTTATTGTCTTTGCTACCTTTGGTTGTTGTGAATGCAGCTAATGAGAGTACAAGAAAGCGTGTAATTTTCTACACAGTGGGAGAGTATTTGTTGCTCGCTGTTGCAAGCGGGCTTCAGGTCGTTTACATTCGTCGCCTCTTCAGCAAGTCGGTTGCATATAACCGGGTCTGAGCATCCATCCATCCATCCATCGATCCTAGTGGTAACATAACATCGTTGCCCCTTTCTTAGCTTTTAGGAAAACCTCAGGAATGAATTATATTAGTCTATCATTTGTTGTATAATCACTTTTGATTGTGCTTACAGTTCATAATTGTAAGCTGGGGGTTCACGTATTAGCATCTTCTTGAAGCTAGGGAAAGCCTATTTATTTACCCTATTATGCTTCAAAAAGGATGTATGTGGGCTCCAAATACCCTTCCTCATTCTTTGCCTCCCTTTTTATCAAAAAGTTCAATTTTTTATATACGCTACAAACTACATCAATACACCACTGCTTACTGAATCAACACCATGTGACATGTGTTGAGGCCAAGTTGATTATAGTTTATTGGATTAGATACCAATTATCATGTGAACGTTGATGCTCGATTGTGAGGCTAATTTGATCATAGTTTAGTAGATTAGATACCA

mRNA sequence

ATTTCCGACCAAAAGTTCTAAACAGAAGAAAACCGAAAGGGTGAGAAAATGGAGAGGAGACCGAGAGAGCGGGGATTCGGATACCTGTTGACGGGTCTTCTTCTGGCGACCTTAATCGCCAGAATCGATTCGCTTTCGGTGACAGTGAACGAGGTTGAATGCATCCATGAATACGTATTGTACGAAGGCGACACCGTTTCTGGGAATTTTGTCGTCGTCGATCATGAAATTTTCTGGGGCGCCGATCATCCTGGCATTGATTTCACTGTTACATCTCCCGCAGGTAATGTGGTGCACTCGTTGAAGGAGACATCTGGTGGCAAGTTTGAGTTTAAGGCTTCTCGCAGTGGAATGTTTAGATTCTGTTTTCGTAATCCCTACTCGACCACCGAGACAGTTTCTTTCTACATCCATGTTGGTCACATTCCAAACGAACATGACCTTGCTAAAGATGAACACCTAGACCCTGTAAATGTTAAAATTGCAGAGCTTAGAGAGGCATTGGAATCGGTCACCGCCGAGCAGAAATATTTGAAAGCACGTGATGCACGCCACCGTCACACTAATGAGAGTACAAGAAAGCGTGTAATTTTCTACACAGTGGGAGAGTATTTGTTGCTCGCTGTTGCAAGCGGGCTTCAGGTCGTTTACATTCGTCGCCTCTTCAGCAAGTCGGTTGCATATAACCGGGTCTGAGCATCCATCCATCCATCCATCGATCCTAGTGGTAACATAACATCGTTGCCCCTTTCTTAGCTTTTAGGAAAACCTCAGGAATGAATTATATTAGTCTATCATTTGTTGTATAATCACTTTTGATTGTGCTTACAGTTCATAATTGTAAGCTGGGGGTTCACGTATTAGCATCTTCTTGAAGCTAGGGAAAGCCTATTTATTTACCCTATTATGCTTCAAAAAGGATGTATGTGGGCTCCAAATACCCTTCCTCATTCTTTGCCTCCCTTTTTATCAAAAAGTTCAATTTTTTATATACGCTACAAACTACATCAATACACCACTGCTTACTGAATCAACACCATGTGACATGTGTTGAGGCCAAGTTGATTATAGTTTATTGGATTAGATACCAATTATCATGTGAACGTTGATGCTCGATTGTGAGGCTAATTTGATCATAGTTTAGTAGATTAGATACCA

Coding sequence (CDS)

ATGGAGAGGAGACCGAGAGAGCGGGGATTCGGATACCTGTTGACGGGTCTTCTTCTGGCGACCTTAATCGCCAGAATCGATTCGCTTTCGGTGACAGTGAACGAGGTTGAATGCATCCATGAATACGTATTGTACGAAGGCGACACCGTTTCTGGGAATTTTGTCGTCGTCGATCATGAAATTTTCTGGGGCGCCGATCATCCTGGCATTGATTTCACTGTTACATCTCCCGCAGGTAATGTGGTGCACTCGTTGAAGGAGACATCTGGTGGCAAGTTTGAGTTTAAGGCTTCTCGCAGTGGAATGTTTAGATTCTGTTTTCGTAATCCCTACTCGACCACCGAGACAGTTTCTTTCTACATCCATGTTGGTCACATTCCAAACGAACATGACCTTGCTAAAGATGAACACCTAGACCCTGTAAATGTTAAAATTGCAGAGCTTAGAGAGGCATTGGAATCGGTCACCGCCGAGCAGAAATATTTGAAAGCACGTGATGCACGCCACCGTCACACTAATGAGAGTACAAGAAAGCGTGTAATTTTCTACACAGTGGGAGAGTATTTGTTGCTCGCTGTTGCAAGCGGGCTTCAGGTCGTTTACATTCGTCGCCTCTTCAGCAAGTCGGTTGCATATAACCGGGTCTGA

Protein sequence

MERRPRERGFGYLLTGLLLATLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHEIFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFYIHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRVIFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV
Homology
BLAST of CmaCh14G017480 vs. ExPASy Swiss-Prot
Match: Q9LIL4 (Transmembrane emp24 domain-containing protein p24beta3 OS=Arabidopsis thaliana OX=3702 GN=At3g22845 PE=2 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 3.9e-95
Identity = 173/215 (80.47%), Postives = 192/215 (89.30%), Query Frame = 0

Query: 1   MERRPRERGFGYLLTGLLLATLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MERR + +   ++L GL+L   I +I SLSVTVN+ EC+ EYVLYEGDTVSGNFVVVDH+
Sbjct: 1   MERR-QAKIHVFVLIGLILLNSINQISSLSVTVNDEECVQEYVLYEGDTVSGNFVVVDHD 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFY 120
           IFWG+DHPG+DFTVTSPAGN+V +LK TSG KFEFKA +SGM++FCF NPYST ETVSFY
Sbjct: 61  IFWGSDHPGLDFTVTSPAGNIVQTLKGTSGDKFEFKAPKSGMYKFCFHNPYSTPETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESV AEQKYLKARD RHRHTNESTRKRV
Sbjct: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVVAEQKYLKARDTRHRHTNESTRKRV 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEY+ LA ASGLQV+YIR+LFSKSVAYNRV
Sbjct: 181 IFYTVGEYIFLAAASGLQVLYIRKLFSKSVAYNRV 214

BLAST of CmaCh14G017480 vs. ExPASy Swiss-Prot
Match: Q9S7M9 (Transmembrane emp24 domain-containing protein p24beta2 OS=Arabidopsis thaliana OX=3702 GN=At3g07680 PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.8e-26
Identity = 63/183 (34.43%), Postives = 97/183 (53.01%), Query Frame = 0

Query: 33  VNEVECIHEYVLYEGDTVSGNFVVVDHEIFWGADHPGIDFTVTSPAGNVVHSLKETSGGK 92
           ++  EC      YEGDT+  +FVV+  +  W  +  G+D  +  P G  +H  +E    K
Sbjct: 26  IDREECFSHKAEYEGDTLHVSFVVIKSDSQWHFNEDGVDLVIHGPTGEQIHDFREQISAK 85

Query: 93  FEFKASRSGMFRFCFRNPYSTTETVSFYIHVGHIPNEHDLAKDEHLDPVNVKIAELREAL 152
            +F   + G++RFCF N     ET+ F + +GH       AKDEH  P+  +I++L EAL
Sbjct: 86  HDFVVQKKGVYRFCFTNKSPYHETIDFDVQLGHFAYYDQHAKDEHFTPLMEQISKLEEAL 145

Query: 153 ESVTAEQKYLKARDARHRHTNESTRKRVIFYTVGEYLLLAVASGLQVVYIRRLFSKSVAY 212
            ++  EQ +L+A+  R    NE+  KR +   + E   L  AS LQV  +RRLF + +  
Sbjct: 146 YNIQFEQHWLEAQTDRQAIVNENMSKRAVHKALFESFALIGASFLQVYLLRRLFERKLGM 205

Query: 213 NRV 216
           +RV
Sbjct: 206 SRV 208

BLAST of CmaCh14G017480 vs. ExPASy Swiss-Prot
Match: Q769F9 (Transmembrane emp24 domain-containing protein A OS=Dictyostelium discoideum OX=44689 GN=empA PE=3 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.7e-18
Identity = 64/181 (35.36%), Postives = 97/181 (53.59%), Query Frame = 0

Query: 28  SLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHEIFWGADHPGIDFTVTSPAGNVVHSLKE 87
           S  V+    ECI+E +      V  +F  +   I  G +   IDFT+ SP   +V+S + 
Sbjct: 26  SFKVSAKVEECIYEEI-----GVDLSFTAMFQVIQGGFN--DIDFTIISPDKRIVYSGQR 85

Query: 88  TSGGKFEFKASRSGMFRFCFRNPYS--TTETVSFYIHVGHIPNEHDLAKDEHLDPVNVKI 147
            S G    ++S +G++ FCF N  S  T +TVSF + VG      ++AK + L P+   I
Sbjct: 86  ESEGTKTLRSSFAGVYSFCFSNKMSSLTDKTVSFILSVGESSPIREIAKKKDLTPIERSI 145

Query: 148 AELREALESVTAEQKYLKARDARHRHTNESTRKRVIFYTVGEYLLLAVASGLQVVYIRRL 207
             L + + +V  EQ Y K R+A HR+T EST  RV++++V E  +L   S  Q+ Y+RR 
Sbjct: 146 MTLSDGVIAVKNEQNYFKMREAAHRNTAESTNSRVLWWSVFEAFVLIALSIWQIYYLRRF 199

BLAST of CmaCh14G017480 vs. ExPASy Swiss-Prot
Match: A7SXK3 (Transmembrane emp24 domain-containing protein OS=Nematostella vectensis OX=45351 GN=v1g194562 PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.7e-13
Identity = 49/180 (27.22%), Postives = 91/180 (50.56%), Query Frame = 0

Query: 29  LSVTVNEVECIHEYVLYEGDTVSGNFVVVDHEIFWGADHPGIDFTVTSPAGNVVHSLKET 88
           +++  +  EC H+ V       SG  + +  E+  G     ID  +  P   V++S +  
Sbjct: 25  ITIDAHGEECFHDKV------TSGTKMGLIFEVAEGG-FLDIDVKIIGPDQKVIYSGERE 84

Query: 89  SGGKFEFKASRSGMFRFCFRNPYS--TTETVSFYIHVGHIPNEHDLAKDEHLDPVNVKIA 148
           + GK+ F A   G + +CF N  S  T + + F + +G  P +     +   D ++  ++
Sbjct: 85  TSGKYTFAAHMDGTYNYCFSNKMSTMTPKVLKFSMDIGEAPKDTSKEDNAGHDKLSEMVS 144

Query: 149 ELREALESVTAEQKYLKARDARHRHTNESTRKRVIFYTVGEYLLLAVASGLQVVYIRRLF 207
           +L EA+  V  EQ+Y++ R+  HR  N++T  RV++++  E L+L   +  QV Y++R F
Sbjct: 145 QLSEAMTGVKHEQEYMEVRERIHRSINDNTNSRVVWWSFFESLVLVAMTLGQVYYLKRFF 197

BLAST of CmaCh14G017480 vs. ExPASy Swiss-Prot
Match: Q28735 (Transmembrane emp24 domain-containing protein 10 OS=Oryctolagus cuniculus OX=9986 GN=TMED10 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.7e-13
Identity = 53/183 (28.96%), Postives = 88/183 (48.09%), Query Frame = 0

Query: 28  SLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHEIFWGADHPGIDFTVTSPAGNVVHSLKE 87
           S  + VN  +C+ E + ++   V+G + + D     GA        +T  AG++++S ++
Sbjct: 33  SFHLPVNSRKCLREEI-HKDLLVTGAYEITDQS--GGAGGLRTHLKITDSAGHILYSKED 92

Query: 88  TSGGKFEFKASRSGMFRFCFRNPYSTTETVSFYI----HVGHIPNEHDLAKDEHLDPVNV 147
            S GKF F      MF  CF +  +        I    H     N  ++AK E L P+ V
Sbjct: 93  ASKGKFAFTTEDYDMFEVCFESKGTGRIPDQLVILDMKHGVEAKNYEEIAKVEKLKPLEV 152

Query: 148 KIAELREALESVTAEQKYLKARDARHRHTNESTRKRVIFYTVGEYLLLAVASGLQVVYIR 207
           ++  L +  ES+  +  Y+K R+   R TNEST  RV+++++     L   +  QV Y+R
Sbjct: 153 ELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRVLYFSIFSMFCLIGLATWQVFYLR 212

BLAST of CmaCh14G017480 vs. ExPASy TrEMBL
Match: A0A6J1IXM6 (transmembrane emp24 domain-containing protein p24beta3-like OS=Cucurbita maxima OX=3661 GN=LOC111480098 PE=3 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 1.4e-116
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0

Query: 1   MERRPRERGFGYLLTGLLLATLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MERRPRERGFGYLLTGLLLATLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE
Sbjct: 1   MERRPRERGFGYLLTGLLLATLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFY 120
           IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFY
Sbjct: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV
Sbjct: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV
Sbjct: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 215

BLAST of CmaCh14G017480 vs. ExPASy TrEMBL
Match: A0A6J1GUU7 (transmembrane emp24 domain-containing protein p24beta3-like OS=Cucurbita moschata OX=3662 GN=LOC111457734 PE=3 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 5.1e-114
Identity = 210/215 (97.67%), Postives = 212/215 (98.60%), Query Frame = 0

Query: 1   MERRPRERGFGYLLTGLLLATLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MER PRERGFGYLL G LLA+LIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE
Sbjct: 1   MERTPRERGFGYLLAGFLLASLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFY 120
           IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGM+RFCFRNPYSTTETVSFY
Sbjct: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMYRFCFRNPYSTTETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV
Sbjct: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV
Sbjct: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 215

BLAST of CmaCh14G017480 vs. ExPASy TrEMBL
Match: A0A6J1FH03 (transmembrane emp24 domain-containing protein p24beta3-like OS=Cucurbita moschata OX=3662 GN=LOC111445313 PE=3 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 8.7e-106
Identity = 194/215 (90.23%), Postives = 204/215 (94.88%), Query Frame = 0

Query: 1   MERRPRERGFGYLLTGLLLATLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MERR RE GFGYLL GLLL +L   IDS+SVTVNE+EC++EYVLYEGDTVSGNFVVVDH+
Sbjct: 1   MERRLRETGFGYLLAGLLLVSLFTGIDSISVTVNELECVYEYVLYEGDTVSGNFVVVDHD 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFY 120
           IFWGADHPGIDF+VTSPAGNVVHSLKETSG KFEFKA RSGM++FCF NPYST ETVSFY
Sbjct: 61  IFWGADHPGIDFSVTSPAGNVVHSLKETSGDKFEFKAPRSGMYKFCFHNPYSTPETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNEHDLAKDEHLDP+NVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV
Sbjct: 121 IHVGHIPNEHDLAKDEHLDPINVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV
Sbjct: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 215

BLAST of CmaCh14G017480 vs. ExPASy TrEMBL
Match: A0A6J1IDU7 (transmembrane emp24 domain-containing protein p24beta3-like OS=Cucurbita maxima OX=3661 GN=LOC111473076 PE=3 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 2.5e-105
Identity = 194/215 (90.23%), Postives = 204/215 (94.88%), Query Frame = 0

Query: 1   MERRPRERGFGYLLTGLLLATLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MERR RE G GYLL GLLL +L A IDS+SVTVNE+EC++EYVLYEGDTVSGNFVVVDH+
Sbjct: 1   MERRLRETGCGYLLAGLLLVSLFAGIDSISVTVNELECVYEYVLYEGDTVSGNFVVVDHD 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFY 120
           IFWGADHPGIDF+VTSPAGNVVHSLKETSG KFEFKA RSGM++FCF NPYST ETVSFY
Sbjct: 61  IFWGADHPGIDFSVTSPAGNVVHSLKETSGDKFEFKAPRSGMYKFCFHNPYSTPETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNEHDLAKDEHLDP+NVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV
Sbjct: 121 IHVGHIPNEHDLAKDEHLDPINVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV
Sbjct: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 215

BLAST of CmaCh14G017480 vs. ExPASy TrEMBL
Match: A0A5A7U264 (Transmembrane emp24 domain-containing protein p24beta3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00810 PE=3 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 2.8e-104
Identity = 191/215 (88.84%), Postives = 204/215 (94.88%), Query Frame = 0

Query: 1   MERRPRERGFGYLLTGLLLATLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MERR RE+G GYLL  LLL +LIA IDS+SVTVNE+EC++EYVLYEGD+VSGNFVVVDH+
Sbjct: 1   MERRAREKGLGYLLAALLLLSLIAGIDSISVTVNEIECVYEYVLYEGDSVSGNFVVVDHD 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFY 120
           IFWGADHPGIDFTVTSPAGNVVHSLKETSG KFEFKA RSGM++FCF NPYST ETVSFY
Sbjct: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGDKFEFKAPRSGMYKFCFHNPYSTPETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNE+D AKDEHLDP+NVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV
Sbjct: 121 IHVGHIPNENDHAKDEHLDPINVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEYLLLAVASGLQV+YIRRLFSKSVAYNRV
Sbjct: 181 IFYTVGEYLLLAVASGLQVIYIRRLFSKSVAYNRV 215

BLAST of CmaCh14G017480 vs. NCBI nr
Match: XP_022980825.1 (transmembrane emp24 domain-containing protein p24beta3-like [Cucurbita maxima])

HSP 1 Score: 428.7 bits (1101), Expect = 2.9e-116
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0

Query: 1   MERRPRERGFGYLLTGLLLATLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MERRPRERGFGYLLTGLLLATLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE
Sbjct: 1   MERRPRERGFGYLLTGLLLATLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFY 120
           IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFY
Sbjct: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV
Sbjct: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV
Sbjct: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 215

BLAST of CmaCh14G017480 vs. NCBI nr
Match: KAG7018742.1 (Transmembrane emp24 domain-containing protein p24beta3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 420.6 bits (1080), Expect = 8.0e-114
Identity = 210/215 (97.67%), Postives = 213/215 (99.07%), Query Frame = 0

Query: 1   MERRPRERGFGYLLTGLLLATLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MER PRERGFGYLL GLLLA+LIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE
Sbjct: 1   MERTPRERGFGYLLAGLLLASLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFY 120
           IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGM++FCFRNPYSTTETVSFY
Sbjct: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMYKFCFRNPYSTTETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV
Sbjct: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV
Sbjct: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 215

BLAST of CmaCh14G017480 vs. NCBI nr
Match: XP_022955882.1 (transmembrane emp24 domain-containing protein p24beta3-like [Cucurbita moschata])

HSP 1 Score: 420.2 bits (1079), Expect = 1.0e-113
Identity = 210/215 (97.67%), Postives = 212/215 (98.60%), Query Frame = 0

Query: 1   MERRPRERGFGYLLTGLLLATLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MER PRERGFGYLL G LLA+LIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE
Sbjct: 1   MERTPRERGFGYLLAGFLLASLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFY 120
           IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGM+RFCFRNPYSTTETVSFY
Sbjct: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMYRFCFRNPYSTTETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV
Sbjct: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV
Sbjct: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 215

BLAST of CmaCh14G017480 vs. NCBI nr
Match: XP_023527186.1 (transmembrane emp24 domain-containing protein p24beta3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 418.7 bits (1075), Expect = 3.0e-113
Identity = 209/215 (97.21%), Postives = 213/215 (99.07%), Query Frame = 0

Query: 1   MERRPRERGFGYLLTGLLLATLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MER PRERGFGYLL GLLLA+LIARIDSLSVTV+EVECIHEYVLYEGDTVSGNFVVVDHE
Sbjct: 1   MERTPRERGFGYLLAGLLLASLIARIDSLSVTVSEVECIHEYVLYEGDTVSGNFVVVDHE 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFY 120
           IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGM+RFCFRNPYSTTETVSFY
Sbjct: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMYRFCFRNPYSTTETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKR+
Sbjct: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRL 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV
Sbjct: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 215

BLAST of CmaCh14G017480 vs. NCBI nr
Match: XP_038897452.1 (transmembrane emp24 domain-containing protein p24beta3 [Benincasa hispida])

HSP 1 Score: 398.3 bits (1022), Expect = 4.3e-107
Identity = 195/215 (90.70%), Postives = 207/215 (96.28%), Query Frame = 0

Query: 1   MERRPRERGFGYLLTGLLLATLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MERR RE+G GYLL GLLL +LIARIDS+SVTVNE+EC++EYVLYEGDTVSGNFVVVDH+
Sbjct: 1   MERRAREKGIGYLLAGLLLVSLIARIDSISVTVNEIECVYEYVLYEGDTVSGNFVVVDHD 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFY 120
           IFWGADHPGIDFTVTSPAGNVVHSLKETSG KFEFKA RSGM++FCF NPYST ETVSFY
Sbjct: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGDKFEFKAPRSGMYKFCFHNPYSTPETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNEH+LAKDEHLDP+NVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV
Sbjct: 121 IHVGHIPNEHNLAKDEHLDPINVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEYLLLAVASGLQV+YIRRLFSKSVAYNRV
Sbjct: 181 IFYTVGEYLLLAVASGLQVIYIRRLFSKSVAYNRV 215

BLAST of CmaCh14G017480 vs. TAIR 10
Match: AT3G22845.1 (emp24/gp25L/p24 family/GOLD family protein )

HSP 1 Score: 349.0 bits (894), Expect = 2.8e-96
Identity = 173/215 (80.47%), Postives = 192/215 (89.30%), Query Frame = 0

Query: 1   MERRPRERGFGYLLTGLLLATLIARIDSLSVTVNEVECIHEYVLYEGDTVSGNFVVVDHE 60
           MERR + +   ++L GL+L   I +I SLSVTVN+ EC+ EYVLYEGDTVSGNFVVVDH+
Sbjct: 1   MERR-QAKIHVFVLIGLILLNSINQISSLSVTVNDEECVQEYVLYEGDTVSGNFVVVDHD 60

Query: 61  IFWGADHPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFY 120
           IFWG+DHPG+DFTVTSPAGN+V +LK TSG KFEFKA +SGM++FCF NPYST ETVSFY
Sbjct: 61  IFWGSDHPGLDFTVTSPAGNIVQTLKGTSGDKFEFKAPKSGMYKFCFHNPYSTPETVSFY 120

Query: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRV 180
           IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESV AEQKYLKARD RHRHTNESTRKRV
Sbjct: 121 IHVGHIPNEHDLAKDEHLDPVNVKIAELREALESVVAEQKYLKARDTRHRHTNESTRKRV 180

Query: 181 IFYTVGEYLLLAVASGLQVVYIRRLFSKSVAYNRV 216
           IFYTVGEY+ LA ASGLQV+YIR+LFSKSVAYNRV
Sbjct: 181 IFYTVGEYIFLAAASGLQVLYIRKLFSKSVAYNRV 214

BLAST of CmaCh14G017480 vs. TAIR 10
Match: AT3G07680.1 (emp24/gp25L/p24 family/GOLD family protein )

HSP 1 Score: 119.8 bits (299), Expect = 2.7e-27
Identity = 63/183 (34.43%), Postives = 97/183 (53.01%), Query Frame = 0

Query: 33  VNEVECIHEYVLYEGDTVSGNFVVVDHEIFWGADHPGIDFTVTSPAGNVVHSLKETSGGK 92
           ++  EC      YEGDT+  +FVV+  +  W  +  G+D  +  P G  +H  +E    K
Sbjct: 26  IDREECFSHKAEYEGDTLHVSFVVIKSDSQWHFNEDGVDLVIHGPTGEQIHDFREQISAK 85

Query: 93  FEFKASRSGMFRFCFRNPYSTTETVSFYIHVGHIPNEHDLAKDEHLDPVNVKIAELREAL 152
            +F   + G++RFCF N     ET+ F + +GH       AKDEH  P+  +I++L EAL
Sbjct: 86  HDFVVQKKGVYRFCFTNKSPYHETIDFDVQLGHFAYYDQHAKDEHFTPLMEQISKLEEAL 145

Query: 153 ESVTAEQKYLKARDARHRHTNESTRKRVIFYTVGEYLLLAVASGLQVVYIRRLFSKSVAY 212
            ++  EQ +L+A+  R    NE+  KR +   + E   L  AS LQV  +RRLF + +  
Sbjct: 146 YNIQFEQHWLEAQTDRQAIVNENMSKRAVHKALFESFALIGASFLQVYLLRRLFERKLGM 205

Query: 213 NRV 216
           +RV
Sbjct: 206 SRV 208

BLAST of CmaCh14G017480 vs. TAIR 10
Match: AT1G57620.1 (emp24/gp25L/p24 family/GOLD family protein )

HSP 1 Score: 75.5 bits (184), Expect = 5.9e-14
Identity = 57/207 (27.54%), Postives = 102/207 (49.28%), Query Frame = 0

Query: 15  TGLLLATLIARIDS------LSVTVNEVECIHEYVLYEGDTVSGNFVVV--DHEIFWGAD 74
           T +LL+ LI  +        L+V     +C+ E +      V  +++V+  +H IF    
Sbjct: 8   TTILLSALIFSLSPICEAVWLTVPHTGSKCVSEEI-QSNVIVLADYLVISEEHSIF---- 67

Query: 75  HPGIDFTVTSPAGNVVHSLKETSGGKFEFKASRSGMFRFCFRNPYSTTETVSFYIHV--- 134
            P +   VT+P G V+H  + T+ G+F F    SG +  CF     +     F I++   
Sbjct: 68  -PTVSVKVTAPYGTVLHHRENTTNGQFAFTTQESGTYLACFEADAKSHGNKDFSINIDWK 127

Query: 135 -GHIPNEHD-LAKDEHLDPVNVKIAELREALESVTAEQKYLKARDARHRHTNESTRKRVI 194
            G    + D +A+ E ++ V ++  +L  A+E++     YL+ R+A  R  +E T  RV 
Sbjct: 128 TGIAAKDWDSIARKEKIEGVELEFKKLEGAVEAIHENLIYLRNREAEMRIVSEKTNSRVA 187

Query: 195 FYTVGEYLLLAVASGLQVVYIRRLFSK 209
           +Y++    +  V SGLQ++Y+++ F K
Sbjct: 188 WYSIMSLGICIVVSGLQILYLKQYFEK 208

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LIL43.9e-9580.47Transmembrane emp24 domain-containing protein p24beta3 OS=Arabidopsis thaliana O... [more]
Q9S7M93.8e-2634.43Transmembrane emp24 domain-containing protein p24beta2 OS=Arabidopsis thaliana O... [more]
Q769F91.7e-1835.36Transmembrane emp24 domain-containing protein A OS=Dictyostelium discoideum OX=4... [more]
A7SXK31.7e-1327.22Transmembrane emp24 domain-containing protein OS=Nematostella vectensis OX=45351... [more]
Q287353.7e-1328.96Transmembrane emp24 domain-containing protein 10 OS=Oryctolagus cuniculus OX=998... [more]
Match NameE-valueIdentityDescription
A0A6J1IXM61.4e-116100.00transmembrane emp24 domain-containing protein p24beta3-like OS=Cucurbita maxima ... [more]
A0A6J1GUU75.1e-11497.67transmembrane emp24 domain-containing protein p24beta3-like OS=Cucurbita moschat... [more]
A0A6J1FH038.7e-10690.23transmembrane emp24 domain-containing protein p24beta3-like OS=Cucurbita moschat... [more]
A0A6J1IDU72.5e-10590.23transmembrane emp24 domain-containing protein p24beta3-like OS=Cucurbita maxima ... [more]
A0A5A7U2642.8e-10488.84Transmembrane emp24 domain-containing protein p24beta3 OS=Cucumis melo var. maku... [more]
Match NameE-valueIdentityDescription
XP_022980825.12.9e-116100.00transmembrane emp24 domain-containing protein p24beta3-like [Cucurbita maxima][more]
KAG7018742.18.0e-11497.67Transmembrane emp24 domain-containing protein p24beta3, partial [Cucurbita argyr... [more]
XP_022955882.11.0e-11397.67transmembrane emp24 domain-containing protein p24beta3-like [Cucurbita moschata][more]
XP_023527186.13.0e-11397.21transmembrane emp24 domain-containing protein p24beta3-like [Cucurbita pepo subs... [more]
XP_038897452.14.3e-10790.70transmembrane emp24 domain-containing protein p24beta3 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G22845.12.8e-9680.47emp24/gp25L/p24 family/GOLD family protein [more]
AT3G07680.12.7e-2734.43emp24/gp25L/p24 family/GOLD family protein [more]
AT1G57620.15.9e-1427.54emp24/gp25L/p24 family/GOLD family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 145..165
NoneNo IPR availablePANTHERPTHR22811:SF178BNAC05G29650D PROTEINcoord: 12..211
IPR009038GOLD domainSMARTSM01190EMP24_GP25L_2coord: 28..207
e-value: 1.4E-52
score: 190.7
IPR009038GOLD domainPFAMPF01105EMP24_GP25Lcoord: 33..206
e-value: 3.5E-42
score: 144.4
IPR009038GOLD domainPROSITEPS50866GOLDcoord: 36..123
score: 16.959135
IPR015720Transmembrane emp24 domain-containing proteinPANTHERPTHR22811TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEINcoord: 12..211
IPR036598GOLD domain superfamilySUPERFAMILY101576Supernatant protein factor (SPF), C-terminal domaincoord: 40..132

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh14G017480.1CmaCh14G017480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport
biological_process GO:0007030 Golgi organization
biological_process GO:0006886 intracellular protein transport
cellular_component GO:0030134 COPII-coated ER to Golgi transport vesicle
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005793 endoplasmic reticulum-Golgi intermediate compartment
cellular_component GO:0005794 Golgi apparatus
cellular_component GO:0016021 integral component of membrane