CmaCh14G016830 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh14G016830
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	NEDD8-activating enzyme E1 catalytic subunit
Location	Cma_Chr14: 12533541 .. 12538094 (+)
RNA-Seq Expression	CmaCh14G016830
Synteny	CmaCh14G016830

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTAAACACTTAGAATGTTGGAATACATATGAGACGCCAAGTTCGAAATGTGAGTGTGTTAGAGTTCATAGGAATTAATTCCCAGACCACTTCCAGTCTACCAAAGCTAGTCATCTTAGGGCAGCCAGTTTGTCTCCCCGGATAGGATATCCGGCCCCTCTATTACGCGACCGTAAGATTTTGTTACGGTTAGATACCCTGTTTAGCCAAAGGAGAAAGAAACTGAATCCCAGTCTATGAATGTGATATTAGTAGAAGATTGAATAGTTCAGAGTGAGTGAGTGTAGTTCTGAGTTTTACCAGAGAGAATCAACTGAATCTGCAAGTTGAGAGAGAAAGAGAGCGATGGAGGTTTAAGGACTGAAGAGGGGAATTTGATATGAACGGATGATAGTTGCAGAGGAGAAAGAGGATCAGTTCATAATGCTACACAAGAGGAACACATACTAAGAAGGGAAATTTTCCTCTTTCTTGAACCTTTGTTGGTTTGAAGTTTGATTTAGAGCCACCTTAGTTTTGTTTTGTTTATCCGCACTGTTTCTCGTATCATTTTTTATATCAGTATCGGTTTTTTTTCTTCTTGGTGATATCTTAGAACGAATAAGTTCAAATTATGATGGTTCTTCGTTCAAGAACACAAGCTCGACATTGAACTATTGATCTCCCTCTTCAAGAGTTCCAAAATGACACGAAGAGCTACAATGCAATGCGATGAATTGTTTCATCGATCTCCCTCCTCATGAAAGAGTTCCAAAATGACATGAAGAGCTACAATCCACTAGCTAATTTGAGAGAATTGGGTACCTCTTATCTTCGAAGATCCACAATGACCTAAGATCCTACTAATCTCACCTAATGTGAATGTAGTTCAGTTGGTCGGGTTTGATCTCTTAATTCCATAATTAGTTGAACATTTATTTTCGAACACTAATCTATTTATTTATTTATTTGGCTCACCACGGAATATTAATATGGTAACTCAAATTTAATTAAAAAATAAAAAGAAATGATTTATACTCAAAATTTAGGGTTGTATCACTTGAAATAAAAAATTAATTACACTAATCATAAAATTAAGAACCATATATCTATTTTAAAATTGTTTAGGTATAATTTTTATACGTGTAAAAAAATTAAATGTAATATAATAGACCATTTAATAATAATTAAAAAAAAGGAGTGGAAAGAAGAAAAAAAAATAAATAAAATTTTGGACCGACGCGCGGGATTGGACTTTAACATCTCCCCGCGTGGGAAAAATGGCGCTGGGGTTGAGGGCGCTGCGATTCGACCAACGGCGACTCAGTACTGCGTGAGAACCAAGCTACTTCCACTTCCACCCAAATCCTACCGTCCCTCGCCGCCGTTTCCACTTCCACCAGAAAACCCCTTTTTACCTCTCTCTCTCTCTCTCTCTCCCCGCTTCTCTTTTACACTTCAAGAATTTCCTCCGGCAGCCCAGAGTCGGCGCTGTAAGGCTTTCAAATGACGGATTCTGTCGTCCAATCGAGTCGATCGAGGGACCTCGATAAGCTCCTTCTTAGGCCCGGTAACCTCATCGGCGCAAATTTCGAGCCCGGTTCCCAAGTGGGTACTTCTTCTCTCCGACGTTTTATCAAATACCTTGTGTGAAATACATGGTCTGCAATCGTCTTACGACTAAGTTGATTTGTATTACAGTTGAGGGATGACCTTCAACAGTATGTGAAAGTGCTGGTCATAGGAGCTGGAGGGTTGGGCTGCGAGCTCCTGAAGGACTTGGCTTTGTCGGGTTTCCGGAATCTGGAAGTAATTGACATGGATCGCATTGAAGTTACTAATCTCAATCGTCAGTTTCTCTTCAGGTTCCAACTAATTTCTTGGTTCTTTATGAATCTTTGGAGTTCTATTCGTAGTGTTCTTTTAAAATTTTAAACAACCGGGGCCTGGGTGGTGGGTATAGAATAGAACCTTTACATTTTGTAGGGTGAATACTGTGCTGTAATGAATTGAAAATTGACCTCTATTTTCAAGTTACATCCATTACTGACTGATGATATCTATATTTATGATTTGTGAAGTGTAAAGATTTCACCTGTTCTAGGAATTAGATGGGTCGAATGTTCTTTCTTTTTAACCATTTGTACGAGGATACTGAACCAAATCATGGGCTTAAATAGATTTTCCTCAAAGAAAGTTCTTTTCGGGACATTTTCTTGATACGATATGATTTGATCATGGACTCATTGAGGAAATCACTTTCTCATATGTGCATTTGCATATGGTTTTACAATAGTCATTTAATTAACTATGGCTGTCAGGAGAATGACTCTATGTAACATTGTAGGCTTGATGATGTTGGTAAGCCAAAAGCCGAGGTAGCTGCAAAACGTGTTATGGAGAGAGTTAGTGGTGTCAATATTGTGTGGCATTTTTGTCGGATTGAGGACAAAGAAATTGAATTTTACAACGATTTTCACATAATTGCACTTGGTCTTGATTCCATTGAAGCTCGAAGTTACATCAATTCTGTCGCTTGTAGCTTCCTCGGTATGCTATTCTCCTACTTGCTACATTTTACTAATGTCTCTTCACCATCTTGTGTTCGGTAAATTTTAATGTTATGTCGATGATGAATGCAAACTTCATGTCCTTTATTTTCTCTTTGTAACCTCTAGAGTACGATTCTGATGATAATCCGTTTGAAGAAACAATTAAACCAATGGTAGATGGTGGAACTGAAGGCTTCAAGGGGCATGCAAGGGTAATTTTACCAGGGGTCACGCCATGCTTCGAGTGTACCATCTGGCTTTTTCCACCTCAAGTAAAGTTCCCTTTATGTACTCTAGCAGAAACTCCTAGAACTGCCGCTCATTGTATCGAGTATGCCCACCTAATTAAATGGGATGAGGTAATGCATTAGGTACCATGCTTTTCATTGATGATTGTGATTGTATGCCTTCGAATTACCAATACTTTTTCTTTTAGGTTCATAGTGGCAAAGCTTTTGATCCGGATGATTCAGAACATATGAAATGGGTTTACAGTGAGGTACATAAATTCCTGTAAGATCTGAGAATTCTTTTCAATTCGTGATTTTAAACGTCTAATTTATCTTCCCACATCTATAGGCTGTTAAGAGAGCCGAACTTTTTGGCATACCGGGAGTTACATATTCACTAACTCAGGTACATATTAGTCTGGGAATTTTCACACTAGTGAACTGATACGTCGATTATATTTTATCAGAACTTGTGATTGTTCTTTTAGTAAGTACATATTATCTGCTTGCATTTTTTTTTTAAACTACTCACTCTTTGTTGTTACAAATGCGCCTATTGATTTACATTTTCTAAGTGATTTTTAGGGCGTAGTGAAGAATATCATTCCTGCTATTGCTTCCACCAATGCTATTATATCAGCTGCATGTACACTGGAAACGTTGAAGATCGTATCGGGATGCAGCAAAACGCTATCCAACTACTTAACGTGTGTACTGTTTTCTTCAAACATCTAACAATTCCTTTTCATTTTTTTGTAATATGGGTTTAATAATAATTTTGTGGTGCAACTTAATAATGTTTTTCTTTATTCGTACTCGTAATTATATAGGTAGATAGATAACATGTCAAGCTGCAATGGGACATTTTGATTTATTCTGTGCAGCATTCTTCGAGTACTAAAGTCATAATCCAGCAGTGTCGTTTATGTTATGATTGCTATCTGTTCACCTTTTCATTTGTTTTTGGTTCTTGTTATGATCTGGAAGTTATTTGGTTCTCATCCTTCCTTCTTGAATTGATAAGTCATTCAATGCCTTGTTCATATACTAAACAATTTTGGTTTCTTTCCATTATTTACTTTTTACTTCTTTTAGATTCGAAGTCTAGCCTTTTATAGCCATCAATCTGTTCTTTCTATCTTTTGGAAGTTGACATTAGGATTGCGAGTGAATTACACGATTTTCAATGCATTACTTGCTTTATCATCTATCCTGCGATTGTTCCTTTTATTTGTGGCCTTGATGTCCAAGTGCATTGAGTTTGATGCCAAGTGATCCTTTTCCAGATATAATGGTGCAGAAGGTCTCCACACTAAAGTGACCGAGTTTGTAAAAGATAAGGATTGTCTTGTTTGTGGTCCAGGTGTTCTTATTGAGCTGGATTCTTCGATCTCTTTACAAAAGGTCGGTTATTTAGCTTATGCAAACATATCGTTAATTTCAGATGTTGAGAAATCTTGTAAATCCTTCATGACGTTACTCCCACTTTTTGTGTGAAACAGTTCATTGAACTTCTAGAAGATCATCCCAAGCTCTTGTTATCGAAGGCAAGTGTCACGCATCGAGGGAAGAATCTATACATGCAGGCACCTCCTGTACTTGAAGAGATGACCAGATCAAATTTGAGTGTTCCACTTTTTGACTTGATGGGTAAAGTTTGGAAAGATGTAGTCCATGTGACTGGTGTAGCTTCTAAGAATGATAAGAAAACTTCTTGTCTCCGAAAATTACGTGTGGCGTTTAAGGGGGTTGACGGTGTTACAGATATGGACACTGCAGGTGGGGCTTAA

mRNA sequence

ATGGGGCGCTGCGATTCGACCAACGGCGACTCAGTACTGCGTGAGAACCAAGCTACTTCCACTTCCACCCAAATCCTACCGTCCCTCGCCGCCGTTTCCACTTCCACCAGAAAACCCCTTTTTACCTCTCTCTCTCTCTCTCTCTCCCCGCTTCTCTTTTACACTTCAAGAATTTCCTCCGGCAGCCCAGAGTCGGCGCTGCCCGGTAACCTCATCGGCGCAAATTTCGAGCCCGGTTCCCAATTGAGGGATGACCTTCAACAGTATGTGAAAGTGCTGGTCATAGGAGCTGGAGGGTTGGGCTGCGAGCTCCTGAAGGACTTGGCTTTGTCGGGTTTCCGGAATCTGGAAGTAATTGACATGGATCGCATTGAAGTTACTAATCTCAATCGTCAGTTTCTCTTCAGGCTTGATGATGTTGGTAAGCCAAAAGCCGAGGTAGCTGCAAAACGTGTTATGGAGAGAGTTAGTGGTGTCAATATTGTGTGGCATTTTTGTCGGATTGAGGACAAAGAAATTGAATTTTACAACGATTTTCACATAATTGCACTTGGTCTTGATTCCATTGAAGCTCGAAGTTACATCAATTCTGTCGCTTGTAGCTTCCTCGAGTACGATTCTGATGATAATCCGTTTGAAGAAACAATTAAACCAATGGTAGATGGTGGAACTGAAGGCTTCAAGGGGCATGCAAGGGTAATTTTACCAGGGGTCACGCCATGCTTCGAGTGTACCATCTGGCTTTTTCCACCTCAAGTAAAGTTCCCTTTATGTACTCTAGCAGAAACTCCTAGAACTGCCGCTCATTGTATCGAGTATGCCCACCTAATTAAATGGGATGAGGTTCATAGTGGCAAAGCTTTTGATCCGGATGATTCAGAACATATGAAATGGGTTTACAGTGAGGCTGTTAAGAGAGCCGAACTTTTTGGCATACCGGGAGTTACATATTCACTAACTCAGGGCGTAGTGAAGAATATCATTCCTGCTATTGCTTCCACCAATGCTATTATATCAGCTGCATGTACACTGGAAACGTTGAAGATCGTATCGGGATGCAGCAAAACGCTATCCAACTACTTAACATATAATGGTGCAGAAGGTCTCCACACTAAAGTGACCGAGTTTGTAAAAGATAAGGATTGTCTTGTTTGTGGTCCAGGTGTTCTTATTGAGCTGGATTCTTCGATCTCTTTACAAAAGTTCATTGAACTTCTAGAAGATCATCCCAAGCTCTTGTTATCGAAGGCAAGTGTCACGCATCGAGGGAAGAATCTATACATGCAGGCACCTCCTGTACTTGAAGAGATGACCAGATCAAATTTGAGTGTTCCACTTTTTGACTTGATGGGTAAAGTTTGGAAAGATGTAGTCCATGTGACTGGTGTAGCTTCTAAGAATGATAAGAAAACTTCTTGTCTCCGAAAATTACGTGTGGCGTTTAAGGGGGTTGACGGTGTTACAGATATGGACACTGCAGGTGGGGCTTAA

Coding sequence (CDS)

ATGGGGCGCTGCGATTCGACCAACGGCGACTCAGTACTGCGTGAGAACCAAGCTACTTCCACTTCCACCCAAATCCTACCGTCCCTCGCCGCCGTTTCCACTTCCACCAGAAAACCCCTTTTTACCTCTCTCTCTCTCTCTCTCTCCCCGCTTCTCTTTTACACTTCAAGAATTTCCTCCGGCAGCCCAGAGTCGGCGCTGCCCGGTAACCTCATCGGCGCAAATTTCGAGCCCGGTTCCCAATTGAGGGATGACCTTCAACAGTATGTGAAAGTGCTGGTCATAGGAGCTGGAGGGTTGGGCTGCGAGCTCCTGAAGGACTTGGCTTTGTCGGGTTTCCGGAATCTGGAAGTAATTGACATGGATCGCATTGAAGTTACTAATCTCAATCGTCAGTTTCTCTTCAGGCTTGATGATGTTGGTAAGCCAAAAGCCGAGGTAGCTGCAAAACGTGTTATGGAGAGAGTTAGTGGTGTCAATATTGTGTGGCATTTTTGTCGGATTGAGGACAAAGAAATTGAATTTTACAACGATTTTCACATAATTGCACTTGGTCTTGATTCCATTGAAGCTCGAAGTTACATCAATTCTGTCGCTTGTAGCTTCCTCGAGTACGATTCTGATGATAATCCGTTTGAAGAAACAATTAAACCAATGGTAGATGGTGGAACTGAAGGCTTCAAGGGGCATGCAAGGGTAATTTTACCAGGGGTCACGCCATGCTTCGAGTGTACCATCTGGCTTTTTCCACCTCAAGTAAAGTTCCCTTTATGTACTCTAGCAGAAACTCCTAGAACTGCCGCTCATTGTATCGAGTATGCCCACCTAATTAAATGGGATGAGGTTCATAGTGGCAAAGCTTTTGATCCGGATGATTCAGAACATATGAAATGGGTTTACAGTGAGGCTGTTAAGAGAGCCGAACTTTTTGGCATACCGGGAGTTACATATTCACTAACTCAGGGCGTAGTGAAGAATATCATTCCTGCTATTGCTTCCACCAATGCTATTATATCAGCTGCATGTACACTGGAAACGTTGAAGATCGTATCGGGATGCAGCAAAACGCTATCCAACTACTTAACATATAATGGTGCAGAAGGTCTCCACACTAAAGTGACCGAGTTTGTAAAAGATAAGGATTGTCTTGTTTGTGGTCCAGGTGTTCTTATTGAGCTGGATTCTTCGATCTCTTTACAAAAGTTCATTGAACTTCTAGAAGATCATCCCAAGCTCTTGTTATCGAAGGCAAGTGTCACGCATCGAGGGAAGAATCTATACATGCAGGCACCTCCTGTACTTGAAGAGATGACCAGATCAAATTTGAGTGTTCCACTTTTTGACTTGATGGGTAAAGTTTGGAAAGATGTAGTCCATGTGACTGGTGTAGCTTCTAAGAATGATAAGAAAACTTCTTGTCTCCGAAAATTACGTGTGGCGTTTAAGGGGGTTGACGGTGTTACAGATATGGACACTGCAGGTGGGGCTTAA

Protein sequence

MGRCDSTNGDSVLRENQATSTSTQILPSLAAVSTSTRKPLFTSLSLSLSPLLFYTSRISSGSPESALPGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGVTDMDTAGGA

Homology

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BLAST of CmaCh14G016830 vs. ExPASy Swiss-Prot
Match: O65041 (NEDD8-activating enzyme E1 catalytic subunit OS=Arabidopsis thaliana OX=3702 GN=ECR1 PE=1 SV=2)

HSP 1 Score: 703.4 bits (1814), Expect = 1.9e-201
Identity = 344/429 (80.19%), Postives = 375/429 (87.41%), Query Frame = 0


Query: 69  GNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTN 128
           GNL+   F PG  LRDD++ YV++LVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTN
Sbjct: 26  GNLVDPGFVPGPGLRDDIRDYVRILVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTN 85

Query: 129 LNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLDS 188
           LNRQFLFR++DVGKPKAEVAAKRVMERVSGV IV HF RIEDKEIEFYNDF+IIALGLDS
Sbjct: 86  LNRQFLFRIEDVGKPKAEVAAKRVMERVSGVEIVPHFSRIEDKEIEFYNDFNIIALGLDS 145

Query: 189 IEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWL 248
           IEAR YIN VAC FLEY+ DD P  ETIKPMVDGGTEGFKGHARVILPGVTPCFECTI+L
Sbjct: 146 IEARKYINGVACGFLEYNEDDTPKRETIKPMVDGGTEGFKGHARVILPGVTPCFECTIYL 205

Query: 249 FPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRAE 308
           FPPQVKFPLCTLAETPR AAHCIEYAHLI+W+ VH GK FDPD+ EHMKWVY EA++RAE
Sbjct: 206 FPPQVKFPLCTLAETPRNAAHCIEYAHLIQWETVHRGKTFDPDEPEHMKWVYDEAIRRAE 265

Query: 309 LFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAEG 368
           LFGIPGVTYSLTQGVVKNIIPAIASTNAIISAAC LETLKIVS CSKTL NYLTYNG EG
Sbjct: 266 LFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSACSKTLVNYLTYNGGEG 325

Query: 369 LHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLYM 428
           L+T+VT+F +D +CLVCGPG+LIELD+S++L KFIE+LEDHPKLLLSKASV      LYM
Sbjct: 326 LYTEVTKFERDTECLVCGPGILIELDTSVTLSKFIEMLEDHPKLLLSKASVKQGENTLYM 385

Query: 429 QAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGV-ASKNDKKTSCLRKLRVAFKGVDGV 488
           QAPPVLEE  R  LS PL+DLMG+V KD +HV G  A KN++K SC  K+RV FKG DGV
Sbjct: 386 QAPPVLEEFHRPKLSKPLYDLMGRVQKDTIHVFGQRALKNNEKESCTTKVRVVFKGADGV 445

Query: 489 TDMDTAGGA 497
            DMDTA GA
Sbjct: 446 ADMDTAIGA 454

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BLAST of CmaCh14G016830 vs. ExPASy Swiss-Prot
Match: Q54QG9 (NEDD8-activating enzyme E1 catalytic subunit OS=Dictyostelium discoideum OX=44689 GN=uba3 PE=1 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 1.4e-100
Identity = 193/391 (49.36%), Postives = 254/391 (64.96%), Query Frame = 0


Query: 69  GNLIGANFEPGSQ----LRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRI 128
           G     +FEP ++    + + LQ   KVLVIGAGGLGCE+LK+LALSGFRN++VIDMD I
Sbjct: 25  GPFASPSFEPDTKASPNIMNGLQNDFKVLVIGAGGLGCEILKNLALSGFRNIDVIDMDTI 84

Query: 129 EVTNLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIAL 188
           +++NLNRQFLFR  DVGK KAEVAA  +  R++G N+  H CRI+DK+ ++Y  F I+  
Sbjct: 85  DISNLNRQFLFRRKDVGKSKAEVAAAFINSRITGCNVTPHKCRIQDKDEDYYRQFKIVIA 144

Query: 189 GLDSIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFEC 248
           GLDSIEAR +IN +  + +  +   +   +TI P+VDGGTEGFKG ARVILP ++ CFEC
Sbjct: 145 GLDSIEARRWINGLLVNLVVVNDSGDIEPDTIIPLVDGGTEGFKGQARVILPKISSCFEC 204

Query: 249 TIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDP-----DDSEHMKWV 308
           ++  FPPQV + +CT+A TPR   HCI++A L    +    K FDP     D+ +HM W+
Sbjct: 205 SLDAFPPQVSYAICTIANTPRVPEHCIQWALLFGLQDATLEKPFDPKQFDNDNPDHMNWL 264

Query: 309 YSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSN 368
           +  A KRAE F I GVTY LTQGV KNIIPAIASTNAII+AAC  E  K  +  S  L+N
Sbjct: 265 FECAKKRAEKFNINGVTYKLTQGVAKNIIPAIASTNAIIAAACCNEVFKFCTDSSGYLNN 324

Query: 369 YLTYNGAEGLHTKVTEFVKDKDCLVCGPG-VLIELDSSISLQKFIELLEDHPKLLLSKAS 428
           Y+ YNG  G++T   E+   + C VCG   V  E+D S +L  F+E +    +    K S
Sbjct: 325 YMMYNGLNGVYTFTFEYEIKEGCAVCGTNLVTFEIDKSNTLSTFLEKITTDSRFQFKKPS 384

Query: 429 VTHRGKNLYMQAPPVLEEMTRSNLSVPLFDL 450
           +   G+NLYMQ   +L + T  NL   L +L
Sbjct: 385 LRSNGRNLYMQG--LLHQSTVPNLEKTLSEL 413

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BLAST of CmaCh14G016830 vs. ExPASy Swiss-Prot
Match: Q9V6U8 (Nedd8-activating enzyme E1 catalytic subunit OS=Drosophila melanogaster OX=7227 GN=Uba3 PE=1 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 7.1e-100
Identity = 189/383 (49.35%), Postives = 251/383 (65.54%), Query Frame = 0


Query: 76  FEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLF 135
           F   S+  + LQ   +VL+IGAGGLGCELLKDLAL GF NL VIDMD IE++NLNRQFLF
Sbjct: 35  FAASSENLEFLQTKCQVLIIGAGGLGCELLKDLALMGFGNLHVIDMDTIELSNLNRQFLF 94

Query: 136 RLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLDSIEARSYI 195
           R  D+G  KAE AA+ +  RV    +  HF +I+D +  FY  FH++  GLDSI AR +I
Sbjct: 95  RRTDIGASKAECAARFINARVPTCRVTPHFKKIQDFDESFYQQFHLVVCGLDSIVARRWI 154

Query: 196 NSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKF 255
           N +  S L Y+ D      +I PM+DGGTEGFKG+ARVILPG T C ECT+ LFPPQV +
Sbjct: 155 NGMLLSMLRYEEDGTIDTSSIVPMIDGGTEGFKGNARVILPGFTACIECTLDLFPPQVNY 214

Query: 256 PLCTLAETPRTAAHCIEYAHLIKWDEVHS-GKAFDPDDSEHMKWVYSEAVKRAELFGIPG 315
           PLCT+A TPR   HCIEY  +I+W++ +  G   D DD +H+ W+Y  A++R+  F I G
Sbjct: 215 PLCTIANTPRLPEHCIEYVKIIQWEKQNPFGVPLDGDDPQHIGWIYERALERSNEFNITG 274

Query: 316 VTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAEGLHTKVT 375
           VTY L QGVVK+IIPA+ASTNA I+AAC LE  K+ + C  +++NYL +N  +G++T   
Sbjct: 275 VTYRLVQGVVKHIIPAVASTNAAIAAACALEVFKLATSCYDSMANYLNFNDLDGIYTYTY 334

Query: 376 EFVKDKDCLVCG--PGVL-IELDSSISLQKFIELLEDHPKLLLSKASVT-----HRGKNL 435
           E  K ++CL C   P  L IE  ++ +L+  I+LL D P+  L   ++T      + + L
Sbjct: 335 EAEKSENCLACSNTPQPLPIEDPNTTTLEDVIKLLCDSPRFQLKSPALTTVMKDGKRRTL 394

Query: 436 YMQAPPVLEEMTRSNLSVPLFDL 450
           YM     +EE TR NL+  L +L
Sbjct: 395 YMSGVKSIEEATRKNLTQSLGEL 417

[Close]

BLAST of CmaCh14G016830 vs. ExPASy Swiss-Prot
Match: Q99MI7 (NEDD8-activating enzyme E1 catalytic subunit OS=Rattus norvegicus OX=10116 GN=Uba3 PE=1 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 4.6e-99
Identity = 191/383 (49.87%), Postives = 252/383 (65.80%), Query Frame = 0


Query: 75  NFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFL 134
           +FEP ++    L    KVLVIGAGGLGCELLK+LALSGFR + VIDMD I+V+NLNRQFL
Sbjct: 55  DFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFL 114

Query: 135 FRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLDSIEARSY 194
           FR  DVG+PKAEVAA+ + +RV   N+V HF +I+D    FY  FHII  GLDSI AR +
Sbjct: 115 FRPKDVGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRW 174

Query: 195 INSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVK 254
           IN +  S L Y+ D      +I P++DGGTEGFKG+ARVILPG+T C ECT+ L+PPQV 
Sbjct: 175 INGMLISLLNYE-DGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVN 234

Query: 255 FPLCTLAETPRTAAHCIEYAHLIKWDEVH---SGKAFDPDDSEHMKWVYSEAVKRAELFG 314
           FP+CT+A  PR   HCIEY  +++W +      G   D DD EH++W++ ++V+RA  + 
Sbjct: 235 FPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGDGVPLDGDDPEHIQWIFQKSVERASQYN 294

Query: 315 IPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAEGLHT 374
           I GVTY LTQGVVK IIPA+ASTNA+I+A C  E  KI +     L+NYL +N  +GL+T
Sbjct: 295 IRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYT 354

Query: 375 KVTEFVKDKDCLVCGP-GVLIELDSSISLQKFIELLEDHPKLLLSKASVTH--RGKN--L 434
              E  + ++C  C      I+   S  LQ+ ++ L +   L +   ++T    GKN  L
Sbjct: 355 YTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTL 414

Query: 435 YMQAPPVLEEMTRSNLSVPLFDL 450
           Y+Q+   +EE TR NLS  L +L
Sbjct: 415 YLQSVTSIEERTRPNLSKTLKEL 436

[Close]

BLAST of CmaCh14G016830 vs. ExPASy Swiss-Prot
Match: Q8C878 (NEDD8-activating enzyme E1 catalytic subunit OS=Mus musculus OX=10090 GN=Uba3 PE=1 SV=2)

HSP 1 Score: 362.8 bits (930), Expect = 6.0e-99
Identity = 190/383 (49.61%), Postives = 252/383 (65.80%), Query Frame = 0


Query: 75  NFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFL 134
           +FEP ++    L    KVLVIGAGGLGCELLK+LALSGFR + VIDMD I+V+NLNRQFL
Sbjct: 55  DFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFL 114

Query: 135 FRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLDSIEARSY 194
           FR  DVG+PKAEVAA+ + +RV   N+V HF +I+D    FY  FHII  GLDSI AR +
Sbjct: 115 FRPKDVGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRW 174

Query: 195 INSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVK 254
           IN +  S L Y+ D      +I P++DGGTEGFKG+ARVILPG+T C ECT+ L+PPQV 
Sbjct: 175 INGMLISLLNYE-DGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVN 234

Query: 255 FPLCTLAETPRTAAHCIEYAHLIKWDEVH---SGKAFDPDDSEHMKWVYSEAVKRAELFG 314
           FP+CT+A  PR   HCIEY  +++W +      G   D DD EH++W++ ++++RA  + 
Sbjct: 235 FPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGDGVPLDGDDPEHIQWIFQKSIERASQYN 294

Query: 315 IPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAEGLHT 374
           I GVTY LTQGVVK IIPA+ASTNA+I+A C  E  KI +     L+NYL +N  +GL+T
Sbjct: 295 IRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYT 354

Query: 375 KVTEFVKDKDCLVCGP-GVLIELDSSISLQKFIELLEDHPKLLLSKASVTH--RGKN--L 434
              E  + ++C  C      I+   S  LQ+ ++ L +   L +   ++T    GKN  L
Sbjct: 355 YTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTL 414

Query: 435 YMQAPPVLEEMTRSNLSVPLFDL 450
           Y+Q+   +EE TR NLS  L +L
Sbjct: 415 YLQSVTSIEERTRPNLSKTLKEL 436

[Close]

BLAST of CmaCh14G016830 vs. ExPASy TrEMBL
Match: A0A6J1IQZ5 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucurbita maxima OX=3661 GN=LOC111479191 PE=3 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 2.3e-250
Identity = 429/429 (100.00%), Postives = 429/429 (100.00%), Query Frame = 0


Query: 68  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 127
           PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT
Sbjct: 21  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 80

Query: 128 NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD 187
           NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD
Sbjct: 81  NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD 140

Query: 188 SIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 247
           SIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW
Sbjct: 141 SIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 200

Query: 248 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA 307
           LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA
Sbjct: 201 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA 260

Query: 308 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE 367
           ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE
Sbjct: 261 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE 320

Query: 368 GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLY 427
           GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLY
Sbjct: 321 GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLY 380

Query: 428 MQAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV 487
           MQAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV
Sbjct: 381 MQAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV 440

Query: 488 TDMDTAGGA 497
           TDMDTAGGA
Sbjct: 441 TDMDTAGGA 449

[Close]

BLAST of CmaCh14G016830 vs. ExPASy TrEMBL
Match: A0A6J1GXR3 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111457804 PE=3 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 8.9e-247
Identity = 425/429 (99.07%), Postives = 425/429 (99.07%), Query Frame = 0


Query: 68  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 127
           PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT
Sbjct: 21  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 80

Query: 128 NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD 187
           NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD
Sbjct: 81  NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD 140

Query: 188 SIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 247
           SIEARSYINSVACSFLEYDSDDNP EETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW
Sbjct: 141 SIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 200

Query: 248 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA 307
           LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAV RA
Sbjct: 201 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVTRA 260

Query: 308 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE 367
           ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE
Sbjct: 261 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE 320

Query: 368 GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLY 427
           GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLL KASVTHRGKNLY
Sbjct: 321 GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLLKASVTHRGKNLY 380

Query: 428 MQAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV 487
           MQAPPVLEEMTRSNLSVPLFDLMGKV KDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV
Sbjct: 381 MQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV 440

Query: 488 TDMDTAGGA 497
           TDMDTAGGA
Sbjct: 441 TDMDTAGGA 449

[Close]

BLAST of CmaCh14G016830 vs. ExPASy TrEMBL
Match: A0A0A0L8U2 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_3G149920 PE=3 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 1.7e-242
Identity = 417/429 (97.20%), Postives = 423/429 (98.60%), Query Frame = 0


Query: 68  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 127
           PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT
Sbjct: 21  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 80

Query: 128 NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD 187
           NLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+ HFCRIEDKEIEFYNDFHIIALGLD
Sbjct: 81  NLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFHIIALGLD 140

Query: 188 SIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 247
           SIEARSYINSVACSFLEYDSDDNP EETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW
Sbjct: 141 SIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 200

Query: 248 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA 307
           LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK+FDPDDSEHMKWVYSEA+KRA
Sbjct: 201 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKSFDPDDSEHMKWVYSEALKRA 260

Query: 308 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE 367
           ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAAC LETLKIVSGCSKTLSNYLTYNGAE
Sbjct: 261 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNYLTYNGAE 320

Query: 368 GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLY 427
           GLHTKVTEFVKDKDCLVCGPGVLIELDSSI+LQKFI+LLEDHPKLLLSKASVTHRGKNLY
Sbjct: 321 GLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDHPKLLLSKASVTHRGKNLY 380

Query: 428 MQAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV 487
           MQAPPVLEEMTRSNL VPLFDLMGKV KDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV
Sbjct: 381 MQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV 440

Query: 488 TDMDTAGGA 497
           TDMDTA GA
Sbjct: 441 TDMDTASGA 449

[Close]

BLAST of CmaCh14G016830 vs. ExPASy TrEMBL
Match: A0A1S3AXQ8 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo OX=3656 GN=LOC103483734 PE=3 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 3.9e-242
Identity = 417/429 (97.20%), Postives = 423/429 (98.60%), Query Frame = 0


Query: 68  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 127
           PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT
Sbjct: 21  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 80

Query: 128 NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD 187
           NLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+ HFCRIEDKEIEFYNDFHIIALGLD
Sbjct: 81  NLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFHIIALGLD 140

Query: 188 SIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 247
           SIEARSYINSVACSFLEYDSDDNP EETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW
Sbjct: 141 SIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 200

Query: 248 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA 307
           LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEA+KRA
Sbjct: 201 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEALKRA 260

Query: 308 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE 367
           ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAAC LETLKIVSGCSKTLSNYLTYNGAE
Sbjct: 261 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNYLTYNGAE 320

Query: 368 GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLY 427
           GLHTKVTEFVKDKDCLVCGPGVLIELDSSI+LQKFI+LLED+PKLLLSKASVTHRGKNLY
Sbjct: 321 GLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVTHRGKNLY 380

Query: 428 MQAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV 487
           MQAPPVLEEMTRSNL VPLFDLMGKV KDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV
Sbjct: 381 MQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV 440

Query: 488 TDMDTAGGA 497
           TDMDTA GA
Sbjct: 441 TDMDTASGA 449

[Close]

BLAST of CmaCh14G016830 vs. ExPASy TrEMBL
Match: A0A5D3D1R8 (NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00180 PE=3 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 1.1e-241
Identity = 416/429 (96.97%), Postives = 422/429 (98.37%), Query Frame = 0


Query: 68  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 127
           PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT
Sbjct: 21  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 80

Query: 128 NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD 187
           NLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+ HFCRIEDKEIEFYNDFHIIALGLD
Sbjct: 81  NLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFHIIALGLD 140

Query: 188 SIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 247
           SIEARSYINSVACSFLEYDSDDNP EETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW
Sbjct: 141 SIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 200

Query: 248 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA 307
           LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEA+KRA
Sbjct: 201 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEALKRA 260

Query: 308 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE 367
           ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAAC LETLKIVSGCSKTLSNYLTYNGAE
Sbjct: 261 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNYLTYNGAE 320

Query: 368 GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLY 427
           GLHTKVTEFVKDKDCLVCGPGVLIELDSSI+LQKFI+LLED+PKLLLSKASVTHRGKNLY
Sbjct: 321 GLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDYPKLLLSKASVTHRGKNLY 380

Query: 428 MQAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV 487
           MQAPPVLEEMTRSNL VPLFDLMGKV KDVVHVTGVASKNDKKTSCLRKLRV FKGVDGV
Sbjct: 381 MQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVTFKGVDGV 440

Query: 488 TDMDTAGGA 497
           TDMDTA GA
Sbjct: 441 TDMDTASGA 449

[Close]

BLAST of CmaCh14G016830 vs. NCBI nr
Match: XP_022979511.1 (NEDD8-activating enzyme E1 catalytic subunit [Cucurbita maxima])

HSP 1 Score: 874.4 bits (2258), Expect = 4.7e-250
Identity = 429/429 (100.00%), Postives = 429/429 (100.00%), Query Frame = 0


Query: 68  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 127
           PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT
Sbjct: 21  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 80

Query: 128 NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD 187
           NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD
Sbjct: 81  NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD 140

Query: 188 SIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 247
           SIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW
Sbjct: 141 SIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 200

Query: 248 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA 307
           LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA
Sbjct: 201 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA 260

Query: 308 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE 367
           ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE
Sbjct: 261 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE 320

Query: 368 GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLY 427
           GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLY
Sbjct: 321 GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLY 380

Query: 428 MQAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV 487
           MQAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV
Sbjct: 381 MQAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV 440

Query: 488 TDMDTAGGA 497
           TDMDTAGGA
Sbjct: 441 TDMDTAGGA 449

[Close]

BLAST of CmaCh14G016830 vs. NCBI nr
Match: XP_023528363.1 (NEDD8-activating enzyme E1 catalytic subunit [Cucurbita pepo subsp. pepo])

HSP 1 Score: 867.1 bits (2239), Expect = 7.5e-248
Identity = 427/429 (99.53%), Postives = 427/429 (99.53%), Query Frame = 0


Query: 68  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 127
           PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT
Sbjct: 21  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 80

Query: 128 NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD 187
           NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD
Sbjct: 81  NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD 140

Query: 188 SIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 247
           SIEARSYINSVACSFLEYDSDDNP EETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW
Sbjct: 141 SIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 200

Query: 248 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA 307
           LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA
Sbjct: 201 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA 260

Query: 308 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE 367
           ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE
Sbjct: 261 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE 320

Query: 368 GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLY 427
           GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLY
Sbjct: 321 GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLY 380

Query: 428 MQAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV 487
           MQAPPVLEEMTRSNLSVPLFDLMGKV KDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV
Sbjct: 381 MQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV 440

Query: 488 TDMDTAGGA 497
           TDMDTAGGA
Sbjct: 441 TDMDTAGGA 449

[Close]

BLAST of CmaCh14G016830 vs. NCBI nr
Match: XP_022955969.1 (NEDD8-activating enzyme E1 catalytic subunit [Cucurbita moschata])

HSP 1 Score: 862.4 bits (2227), Expect = 1.8e-246
Identity = 425/429 (99.07%), Postives = 425/429 (99.07%), Query Frame = 0


Query: 68  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 127
           PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT
Sbjct: 21  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 80

Query: 128 NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD 187
           NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD
Sbjct: 81  NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD 140

Query: 188 SIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 247
           SIEARSYINSVACSFLEYDSDDNP EETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW
Sbjct: 141 SIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 200

Query: 248 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA 307
           LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAV RA
Sbjct: 201 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVTRA 260

Query: 308 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE 367
           ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE
Sbjct: 261 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE 320

Query: 368 GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLY 427
           GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLL KASVTHRGKNLY
Sbjct: 321 GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLLKASVTHRGKNLY 380

Query: 428 MQAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV 487
           MQAPPVLEEMTRSNLSVPLFDLMGKV KDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV
Sbjct: 381 MQAPPVLEEMTRSNLSVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV 440

Query: 488 TDMDTAGGA 497
           TDMDTAGGA
Sbjct: 441 TDMDTAGGA 449

[Close]

BLAST of CmaCh14G016830 vs. NCBI nr
Match: KAG7018684.1 (NEDD8-activating enzyme E1 catalytic subunit [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 850.1 bits (2195), Expect = 9.4e-243
Identity = 427/462 (92.42%), Postives = 427/462 (92.42%), Query Frame = 0


Query: 68  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 127
           PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT
Sbjct: 21  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 80

Query: 128 NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD 187
           NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD
Sbjct: 81  NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD 140

Query: 188 SIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 247
           SIEARSYINSVACSFLEYDSDDNP EETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW
Sbjct: 141 SIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 200

Query: 248 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA 307
           LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA
Sbjct: 201 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA 260

Query: 308 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE 367
           ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE
Sbjct: 261 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE 320

Query: 368 GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQK-------------------------- 427
           GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQK                          
Sbjct: 321 GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKVGYLAYANISLISDVEKSCKILHDVT 380

Query: 428 -------FIELLEDHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVW 487
                  FIELLEDHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKV 
Sbjct: 381 PTFCVKQFIELLEDHPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPLFDLMGKVL 440

Query: 488 KDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGVTDMDTAGGA 497
           KDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGVTDMDTAGGA
Sbjct: 441 KDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGVTDMDTAGGA 482

[Close]

BLAST of CmaCh14G016830 vs. NCBI nr
Match: XP_038897781.1 (NEDD8-activating enzyme E1 catalytic subunit [Benincasa hispida])

HSP 1 Score: 850.1 bits (2195), Expect = 9.4e-243
Identity = 419/429 (97.67%), Postives = 422/429 (98.37%), Query Frame = 0


Query: 68  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 127
           PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT
Sbjct: 21  PGNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 80

Query: 128 NLNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLD 187
           NLNRQFLFRL+DVGKPKAEVAAKRVMERVSGVNIV HFCRIEDKEIEFYNDFHIIALGLD
Sbjct: 81  NLNRQFLFRLEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKEIEFYNDFHIIALGLD 140

Query: 188 SIEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 247
           SIEARSYINSVACSFLEYDSDDNP EETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW
Sbjct: 141 SIEARSYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIW 200

Query: 248 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA 307
           LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA
Sbjct: 201 LFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRA 260

Query: 308 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAE 367
           ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAAC LETLKIVSGCSKTLSNYLTYNGAE
Sbjct: 261 ELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNYLTYNGAE 320

Query: 368 GLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLY 427
           GLHTKVTEFVKDKDCLVCGPGVLIELDSSI+LQKFI+LLEDHPKLLLSKASVTHRGKNLY
Sbjct: 321 GLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDHPKLLLSKASVTHRGKNLY 380

Query: 428 MQAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGVASKNDKKTSCLRKLRVAFKGVDGV 487
           MQAPPVLEEMTRSNL VPLFDLMGKV KD VHVTGVASKNDKKTSCLRKLRVAFKGVDGV
Sbjct: 381 MQAPPVLEEMTRSNLGVPLFDLMGKVLKDAVHVTGVASKNDKKTSCLRKLRVAFKGVDGV 440

Query: 488 TDMDTAGGA 497
           TDMDTA GA
Sbjct: 441 TDMDTASGA 449

[Close]

BLAST of CmaCh14G016830 vs. TAIR 10
Match: AT5G19180.1 (E1 C-terminal related 1 )

HSP 1 Score: 703.4 bits (1814), Expect = 1.3e-202
Identity = 344/429 (80.19%), Postives = 375/429 (87.41%), Query Frame = 0


Query: 69  GNLIGANFEPGSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTN 128
           GNL+   F PG  LRDD++ YV++LVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTN
Sbjct: 26  GNLVDPGFVPGPGLRDDIRDYVRILVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTN 85

Query: 129 LNRQFLFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDKEIEFYNDFHIIALGLDS 188
           LNRQFLFR++DVGKPKAEVAAKRVMERVSGV IV HF RIEDKEIEFYNDF+IIALGLDS
Sbjct: 86  LNRQFLFRIEDVGKPKAEVAAKRVMERVSGVEIVPHFSRIEDKEIEFYNDFNIIALGLDS 145

Query: 189 IEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWL 248
           IEAR YIN VAC FLEY+ DD P  ETIKPMVDGGTEGFKGHARVILPGVTPCFECTI+L
Sbjct: 146 IEARKYINGVACGFLEYNEDDTPKRETIKPMVDGGTEGFKGHARVILPGVTPCFECTIYL 205

Query: 249 FPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRAE 308
           FPPQVKFPLCTLAETPR AAHCIEYAHLI+W+ VH GK FDPD+ EHMKWVY EA++RAE
Sbjct: 206 FPPQVKFPLCTLAETPRNAAHCIEYAHLIQWETVHRGKTFDPDEPEHMKWVYDEAIRRAE 265

Query: 309 LFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKIVSGCSKTLSNYLTYNGAEG 368
           LFGIPGVTYSLTQGVVKNIIPAIASTNAIISAAC LETLKIVS CSKTL NYLTYNG EG
Sbjct: 266 LFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKIVSACSKTLVNYLTYNGGEG 325

Query: 369 LHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIELLEDHPKLLLSKASVTHRGKNLYM 428
           L+T+VT+F +D +CLVCGPG+LIELD+S++L KFIE+LEDHPKLLLSKASV      LYM
Sbjct: 326 LYTEVTKFERDTECLVCGPGILIELDTSVTLSKFIEMLEDHPKLLLSKASVKQGENTLYM 385

Query: 429 QAPPVLEEMTRSNLSVPLFDLMGKVWKDVVHVTGV-ASKNDKKTSCLRKLRVAFKGVDGV 488
           QAPPVLEE  R  LS PL+DLMG+V KD +HV G  A KN++K SC  K+RV FKG DGV
Sbjct: 386 QAPPVLEEFHRPKLSKPLYDLMGRVQKDTIHVFGQRALKNNEKESCTTKVRVVFKGADGV 445

Query: 489 TDMDTAGGA 497
            DMDTA GA
Sbjct: 446 ADMDTAIGA 454

[Close]

BLAST of CmaCh14G016830 vs. TAIR 10
Match: AT2G21470.1 (SUMO-activating enzyme 2 )

HSP 1 Score: 151.0 bits (380), Expect = 2.5e-36
Identity = 79/203 (38.92%), Postives = 118/203 (58.13%), Query Frame = 0


Query: 91  KVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAK 150
           KVL++GAGG+GCELLK LALSGF ++ +IDMD IEV+NLNRQFLFR   VG+ KA+VA  
Sbjct: 14  KVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKAKVARD 73

Query: 151 RVMERVSGVNIVWHFCRIEDKE--IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSD 210
            V+     +NI  +   +++ E  ++F+  F ++  GLD+++AR ++N +  +       
Sbjct: 74  AVLRFRPNINIRSYHANVKNPEFDVDFFKQFDVVLNGLDNLDARRHVNRLCLA------- 133

Query: 211 DNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAA 270
                    P+V+ GT GF G   V + G T C+EC     P    +P+CT+  TP    
Sbjct: 134 ------ADVPLVESGTTGFLGQVTVHIKGKTECYECQ--TKPAPKTYPVCTITSTPTKFV 193

Query: 271 HCIEYAHLIKWDEVHSGKAFDPD 292
           HCI +A  + + ++   K  D D
Sbjct: 194 HCIVWAKDLLFAKLFGDKNQDND 201

HSP 2 Score: 50.4 bits (119), Expect = 4.7e-06
Identity = 44/163 (26.99%), Postives = 80/163 (49.08%), Query Frame = 0


Query: 288 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETL 347
           FD DD   +++V + A  RAE FGIP  +    +G+  NI+ A+A+TNAII+    +E +
Sbjct: 336 FDKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEAI 395

Query: 348 KIV-SGCSKTLSNY-LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIEL 407
           K++     K    Y L +   + L   +  +  +  C VC    L+ L+ +    K  +L
Sbjct: 396 KVLKKDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYVCSETPLV-LEINTRKSKLRDL 455

Query: 408 LED--HPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPL 447
           ++     KL ++   + H G +L  +    L+++  +N +  L
Sbjct: 456 VDKIVKTKLGMNLPLIMH-GNSLLYEVGDDLDDIMVANYNANL 496

[Close]

BLAST of CmaCh14G016830 vs. TAIR 10
Match: AT2G21470.2 (SUMO-activating enzyme 2 )

HSP 1 Score: 151.0 bits (380), Expect = 2.5e-36
Identity = 79/203 (38.92%), Postives = 118/203 (58.13%), Query Frame = 0


Query: 91  KVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAK 150
           KVL++GAGG+GCELLK LALSGF ++ +IDMD IEV+NLNRQFLFR   VG+ KA+VA  
Sbjct: 14  KVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKAKVARD 73

Query: 151 RVMERVSGVNIVWHFCRIEDKE--IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSD 210
            V+     +NI  +   +++ E  ++F+  F ++  GLD+++AR ++N +  +       
Sbjct: 74  AVLRFRPNINIRSYHANVKNPEFDVDFFKQFDVVLNGLDNLDARRHVNRLCLA------- 133

Query: 211 DNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAA 270
                    P+V+ GT GF G   V + G T C+EC     P    +P+CT+  TP    
Sbjct: 134 ------ADVPLVESGTTGFLGQVTVHIKGKTECYECQ--TKPAPKTYPVCTITSTPTKFV 193

Query: 271 HCIEYAHLIKWDEVHSGKAFDPD 292
           HCI +A  + + ++   K  D D
Sbjct: 194 HCIVWAKDLLFAKLFGDKNQDND 201

HSP 2 Score: 50.4 bits (119), Expect = 4.7e-06
Identity = 44/163 (26.99%), Postives = 80/163 (49.08%), Query Frame = 0


Query: 288 FDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACTLETL 347
           FD DD   +++V + A  RAE FGIP  +    +G+  NI+ A+A+TNAII+    +E +
Sbjct: 336 FDKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVHAVATTNAIIAGLIVIEAI 395

Query: 348 KIV-SGCSKTLSNY-LTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSISLQKFIEL 407
           K++     K    Y L +   + L   +  +  +  C VC    L+ L+ +    K  +L
Sbjct: 396 KVLKKDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYVCSETPLV-LEINTRKSKLRDL 455

Query: 408 LED--HPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVPL 447
           ++     KL ++   + H G +L  +    L+++  +N +  L
Sbjct: 456 VDKIVKTKLGMNLPLIMH-GNSLLYEVGDDLDDIMVANYNANL 496

[Close]

BLAST of CmaCh14G016830 vs. TAIR 10
Match: AT2G21470.3 (SUMO-activating enzyme 2 )

HSP 1 Score: 151.0 bits (380), Expect = 2.5e-36
Identity = 79/203 (38.92%), Postives = 118/203 (58.13%), Query Frame = 0


Query: 91  KVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRLDDVGKPKAEVAAK 150
           KVL++GAGG+GCELLK LALSGF ++ +IDMD IEV+NLNRQFLFR   VG+ KA+VA  
Sbjct: 14  KVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQSKAKVARD 73

Query: 151 RVMERVSGVNIVWHFCRIEDKE--IEFYNDFHIIALGLDSIEARSYINSVACSFLEYDSD 210
            V+     +NI  +   +++ E  ++F+  F ++  GLD+++AR ++N +  +       
Sbjct: 74  AVLRFRPNINIRSYHANVKNPEFDVDFFKQFDVVLNGLDNLDARRHVNRLCLA------- 133

Query: 211 DNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWLFPPQVKFPLCTLAETPRTAA 270
                    P+V+ GT GF G   V + G T C+EC     P    +P+CT+  TP    
Sbjct: 134 ------ADVPLVESGTTGFLGQVTVHIKGKTECYECQ--TKPAPKTYPVCTITSTPTKFV 193

Query: 271 HCIEYAHLIKWDEVHSGKAFDPD 292
           HCI +A  + + ++   K  D D
Sbjct: 194 HCIVWAKDLLFAKLFGDKNQDND 201

HSP 2 Score: 53.1 bits (126), Expect = 7.2e-07
Identity = 48/181 (26.52%), Postives = 85/181 (46.96%), Query Frame = 0


Query: 270 CIEYAHLIKWDEVHSGKAFDPDDSEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIP 329
           C+E  HL           FD DD   +++V + A  RAE FGIP  +    +G+  NI+ 
Sbjct: 331 CVEIGHL----------TFDKDDQLAVEFVTAAANIRAESFGIPLHSLFEAKGIAGNIVH 390

Query: 330 AIASTNAIISAACTLETLKIV-SGCSKTLSNY-LTYNGAEGLHTKVTEFVKDKDCLVCGP 389
           A+A+TNAII+    +E +K++     K    Y L +   + L   +  +  +  C VC  
Sbjct: 391 AVATTNAIIAGLIVIEAIKVLKKDVDKFRMTYCLEHPSKKLLLMPIEPYEPNPACYVCSE 450

Query: 390 GVLIELDSSISLQKFIELLED--HPKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLSVP 447
             L+ L+ +    K  +L++     KL ++   + H G +L  +    L+++  +N +  
Sbjct: 451 TPLV-LEINTRKSKLRDLVDKIVKTKLGMNLPLIMH-GNSLLYEVGDDLDDIMVANYNAN 499

[Close]

BLAST of CmaCh14G016830 vs. TAIR 10
Match: AT5G06460.1 (ubiquitin activating enzyme 2 )

HSP 1 Score: 100.1 bits (248), Expect = 5.2e-21
Identity = 67/206 (32.52%), Postives = 109/206 (52.91%), Query Frame = 0


Query: 79  GSQLRDDLQQYVKVLVIGAGGLGCELLKDLALSGFR-----NLEVIDMDRIEVTNLNRQF 138
           GS L+  L+   +V V+GAG LGCE LK+LAL G        L V D D IE +NL+RQF
Sbjct: 480 GSTLQKKLED-ARVFVVGAGALGCEFLKNLALMGVSCGTQGKLTVTDDDVIEKSNLSRQF 539

Query: 139 LFRLDDVGKPKAEVAAKRVMERVSGVNIVWHFCRIEDK-----EIEFYNDFHIIALGLDS 198
           LFR  ++G+ K+ VAA       S +NI     R+  +     +  F+ +  ++   LD+
Sbjct: 540 LFRDWNIGQAKSTVAATAAAGINSRLNIDALQNRVGPETENVFDDSFWENLTVVVNALDN 599

Query: 199 IEARSYINSVACSFLEYDSDDNPFEETIKPMVDGGTEGFKGHARVILPGVTPCFECTIWL 258
           + AR Y++S  C + +            KP+++ GT G K + ++++P +T  +  +   
Sbjct: 600 VTARLYVDS-RCVYFQ------------KPLLESGTLGAKCNTQMVIPHLTENYGAS--R 659

Query: 259 FPPQVKFPLCTLAETPRTAAHCIEYA 275
            PP+ + P+CT+   P    HC+ +A
Sbjct: 660 DPPEKQAPMCTVHSFPHNIDHCLTWA 669

The following BLAST results are available for this feature:

BLAST of CmaCh14G016830 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65041	1.9e-201	80.19	NEDD8-activating enzyme E1 catalytic subunit OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q54QG9	1.4e-100	49.36	NEDD8-activating enzyme E1 catalytic subunit OS=Dictyostelium discoideum OX=4468...	[more]
Q9V6U8	7.1e-100	49.35	Nedd8-activating enzyme E1 catalytic subunit OS=Drosophila melanogaster OX=7227 ...	[more]
Q99MI7	4.6e-99	49.87	NEDD8-activating enzyme E1 catalytic subunit OS=Rattus norvegicus OX=10116 GN=Ub...	[more]
Q8C878	6.0e-99	49.61	NEDD8-activating enzyme E1 catalytic subunit OS=Mus musculus OX=10090 GN=Uba3 PE...	[more]

BLAST of CmaCh14G016830 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IQZ5	2.3e-250	100.00	NEDD8-activating enzyme E1 catalytic subunit OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1GXR3	8.9e-247	99.07	NEDD8-activating enzyme E1 catalytic subunit OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A0A0L8U2	1.7e-242	97.20	NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_3...	[more]
A0A1S3AXQ8	3.9e-242	97.20	NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A5D3D1R8	1.1e-241	96.97	NEDD8-activating enzyme E1 catalytic subunit OS=Cucumis melo var. makuwa OX=1194...	[more]

BLAST of CmaCh14G016830 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022979511.1	4.7e-250	100.00	NEDD8-activating enzyme E1 catalytic subunit [Cucurbita maxima]	[more]
XP_023528363.1	7.5e-248	99.53	NEDD8-activating enzyme E1 catalytic subunit [Cucurbita pepo subsp. pepo]	[more]
XP_022955969.1	1.8e-246	99.07	NEDD8-activating enzyme E1 catalytic subunit [Cucurbita moschata]	[more]
KAG7018684.1	9.4e-243	92.42	NEDD8-activating enzyme E1 catalytic subunit [Cucurbita argyrosperma subsp. argy...	[more]
XP_038897781.1	9.4e-243	97.67	NEDD8-activating enzyme E1 catalytic subunit [Benincasa hispida]	[more]

BLAST of CmaCh14G016830 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19180.1	1.3e-202	80.19	E1 C-terminal related 1	[more]
AT2G21470.1	2.5e-36	38.92	SUMO-activating enzyme 2	[more]
AT2G21470.2	2.5e-36	38.92	SUMO-activating enzyme 2	[more]
AT2G21470.3	2.5e-36	38.92	SUMO-activating enzyme 2	[more]
AT5G06460.1	5.2e-21	32.52	ubiquitin activating enzyme 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014929	E2 binding	SMART	SM01181	E2_bind_2	coord: 391..482 e-value: 6.7E-36 score: 135.3
IPR014929	E2 binding	PFAM	PF08825	E2_bind	coord: 392..481 e-value: 4.0E-22 score: 78.1
None	No IPR available	GENE3D	3.10.290.20		coord: 390..475 e-value: 7.9E-16 score: 59.7
None	No IPR available	GENE3D	3.40.50.720		coord: 88..389 e-value: 5.7E-65 score: 221.2
None	No IPR available	PANTHER	PTHR10953	UBIQUITIN-ACTIVATING ENZYME E1	coord: 68..460
None	No IPR available	CDD	cd01488	Uba3_RUB	coord: 91..386 e-value: 0.0 score: 557.74
IPR000594	THIF-type NAD/FAD binding fold	PFAM	PF00899	ThiF	coord: 83..382 e-value: 1.7E-69 score: 233.9
IPR023318	Ubiquitin activating enzyme, alpha domain superfamily	GENE3D	1.10.10.520	Ubiquitin activating enzymes (Uba3). Chain: B, domain 2	coord: 258..326 e-value: 1.7E-31 score: 110.0
IPR030468	NEDD8-activating enzyme E1 catalytic subunit	PANTHER	PTHR10953:SF6	NEDD8-ACTIVATING ENZYME E1 CATALYTIC SUBUNIT	coord: 68..460
IPR033127	Ubiquitin-activating enzyme E1, Cys active site	PROSITE	PS00865	UBIQUITIN_ACTIVAT_2	coord: 256..264
IPR035985	Ubiquitin-activating enzyme	SUPERFAMILY	69572	Activating enzymes of the ubiquitin-like proteins	coord: 91..455

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh14G016830.1	CmaCh14G016830.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045116	protein neddylation
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0019781	NEDD8 activating enzyme activity
molecular_function	GO:0008641	ubiquitin-like modifier activating enzyme activity