CmaCh14G015770 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh14G015770
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionAlkaline/neutral invertase
LocationCma_Chr14: 11860433 .. 11863291 (+)
RNA-Seq ExpressionCmaCh14G015770
SyntenyCmaCh14G015770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATACTTCTAGTTCTCTGGGAATTTCTACCATGAAACCTTGTAGGATTCTTTTTAGCTTCAAATCCTCCTCGATGTTTGGAACAACCTCATTGCCCAAGGCTAAGTGTAGGAGGATCGGTAGATTTTCGAAATTAGAGCCGAGTGGGCGTAAAATAGTAGGATCTGTACAGGTTGTTGGTGATTTGAATAGGAGATGCTTTAGTTGTTCTAATTTATATCGGTTGTATAAAGGTAACAGTGGTAGAAATAGGTGTTTGATTGCAAATGTAGCTTCGGATTTTAGAAATCAATCGACATCAGCTGAGCCTCATGTGAAACAGAAGAGCTTTGAGAGAATTTATATTCAAGGAGGGTTTAAGGTTAAGCCATTGGTGATTGAGAGTATTGAGACGGATCTTGTGAAAGATGAAAAAAAGGTGTCTGAGGTTGAGGAACTGAGTGGTTTGAAGGGCTCTAGGGTTGAGAGAGAGGTGTCCAAAATTGAAAAGGAGGCATGGATCTTGCTTCGAGACTCTGTTGTGAACTATTGTGGACATCCTGTTGGAACTGTTGCTGCTAATGATCCATCTGATACTCAGCCACTGAATTACGACCAGGTTTTTGTTCGTGATTTTGTACCGTCCGCCTTGGCCTTCTTGTTAAATGGAGAACAGGAGATTGTCAAGAATTTTCTCCTTCACACCTTGCAATTACAGGTATAGACTTACATGTTGAGCTTTTTGTTTATCTTGTATATGCATACCTGGTCTCCTTAATTTGATGCTGAGTTTTAAGTAAAATTTACTGCGGTTGGATGATGAAAGTTCCACATTGGCTAATTTATGGAATGATCATGGGTTTATAACAAATAATTCTCTCTCCATTGGTATGAGGCCTTTTGGGGAAGCCCAAAGCAAAGCCATGAGAGCTTATGCTCAAAGTGGACAATATCATACTATTGTGGAGGGTCGTGTTCGTCTAACATGTTATCAGAGCCGTGCCCTAAACTTAGTCGTGCCAATAGATTGGTAAATCCTCAAATGTCGAAGAAAGAACTCCAAAAGAAATCAACCGTCTATGCTATATCACGACATCTCATCATCCTGAAGTGGAAGCACACTAACATTTTGAGAGACCTTTTTCTTACAAGTCCTAATGATATTTATTAGTACTACAGCTCACATCGTTCTTTGAACTGAAATCAACGGTCTTTATTGTTGATTAGAGTTGGGAGAAAACCGTTGACTGTTACAGCCCTGGGCAAGGGTTGATGCCAGCAAGTTTCAAAGTTAGAAGCCAACCTCTCGATGGAAACGATGGAGCTTTCGAGGAAGTTCTCGATCCTGATTTTGGTGAATCTGCCATTGGTCGTGTTGCACCAGTTGATTCTGGTAACTATAGATTACTGTGCAAACATGCAACTTTACATTTGCCATAGAGTTTGAAACCTTGTTACTGTGTATGCTTGCTCGCAGGGTTGTGGTGGATTATTTTATTAAGAGCTTATGGAAAGATTACGGGAGACTACACATTGCAGGAACGTGTCGATGTACAGACAGGCATAAGACTGATCCTTAATCTCTGCTTGACGAATGGGTTCGACATGTTTCCTACTCTGTTAGTTGGTGATGGCTCGTGCATGATTGATAGACGAATGGGCATTCATGGACACCCACTTGAAATTCAAGTAAGAATACTAATTGATCATCCTTCATAGCATTTGCAGGCAATCCTTCACGGTTACTGTATGTATAGTTAAGATCATTTGCAGGCAGTTCCTTACTGTGGTTAATTTGTTTTGCAGGCATTGTTTTATTCGGCCTTACGCTGCTCCAGAGAGATGCTGATTGTTAACGACTCGACGAAGAATTTGGTGGCTGCCCTGAACAATAGGCTGAGTGCACTCTCCTTCCACATCCGGGAATATTTCTGGGTCGATAAGAACAAACTCAACGAAATTTATCGATATAGAACTGAGGAATATTCTACCGATGCAGTGAACAAGTTCAATATATATCCCGAACAAATTCCTGGTTGGCTGGTGGACTGGATTCCTGAGGAGAGTGGCTACCTGATTGGCAATCTACAGTCTGCTCACATGGACTTCAGGTTTTTTACGCTCGGAAATCTTTGGTCCATTGTTTCGTCACTCGGGACTCCGCAACAGAATGAGGGCATTCTGAACTTGATTGAGGCCAAATGGGATGACCTTGTGGCTAACATGCCTCTCAAGATATGCTTCCCTGCCATGGAACACGAGGAATGGCGCATAATAACTGGAAGCGACCCGAAGAACACGTTAGTCTCTTTAGAGTTTTGAAAAGTAGTCTGATTTGAAATATATCAAAATACTCGTTACCAAGCACTGACAATTATCTCTGCTTTTCCTGTTGGCAGTCCTTGGTCATATCATAATGGAGGATCTTGGCCAACTCTCTTGTGGCAGGTGAGGGACTTTTCTTATTCAAACTTCTGTTCAAACTTGATTAACCATTCATTATTCTCATTGCTATTTTTTGTGGACATCTTTTCAGTTCACACTGGCCTGCATGAAGATGGGGCGGCCAGAGCTAGCAAGGAAGGCCATTGCAGTGGCTGAGAAGAAGCTTTCAGCTGATCGTTGGCCCGAGTACTACGACATGCGCAGTGCAAGCTTAATAGGGAAGCAATCACGCCTCTTCCAAACATGGACGATTGCCGGTTTCTTGACATCAAAGTTGCTTCTGGAGAACCCAGAGAAGGCGTCTCTATTGTTCTGGGAGGAGGATTACGAGCTTCTTCAAGGCTGCGTTTGTGTACTCGGCACAGCCAATGGAAACAAGTGCTCTCGCCATCGTCCTGGCCAGCATCGAAAACCGAATAATCTCAACCATTAG

mRNA sequence

ATGCATACTTCTAGTTCTCTGGGAATTTCTACCATGAAACCTTGTAGGATTCTTTTTAGCTTCAAATCCTCCTCGATGTTTGGAACAACCTCATTGCCCAAGGCTAAGTGTAGGAGGATCGGTAGATTTTCGAAATTAGAGCCGAGTGGGCGTAAAATAGTAGGATCTGTACAGGTTGTTGGTGATTTGAATAGGAGATGCTTTAGTTGTTCTAATTTATATCGGTTGTATAAAGGTAACAGTGGTAGAAATAGGTGTTTGATTGCAAATGTAGCTTCGGATTTTAGAAATCAATCGACATCAGCTGAGCCTCATGTGAAACAGAAGAGCTTTGAGAGAATTTATATTCAAGGAGGGTTTAAGGTTAAGCCATTGGTGATTGAGAGTATTGAGACGGATCTTGTGAAAGATGAAAAAAAGGTGTCTGAGGTTGAGGAACTGAGTGGTTTGAAGGGCTCTAGGGTTGAGAGAGAGGTGTCCAAAATTGAAAAGGAGGCATGGATCTTGCTTCGAGACTCTGTTGTGAACTATTGTGGACATCCTGTTGGAACTGTTGCTGCTAATGATCCATCTGATACTCAGCCACTGAATTACGACCAGGTTTTTGTTCGTGATTTTGTACCGTCCGCCTTGGCCTTCTTGTTAAATGGAGAACAGGAGATTGTCAAGAATTTTCTCCTTCACACCTTGCAATTACAGAGTTGGGAGAAAACCGTTGACTGTTACAGCCCTGGGCAAGGGTTGATGCCAGCAAGTTTCAAAGTTAGAAGCCAACCTCTCGATGGAAACGATGGAGCTTTCGAGGAAGTTCTCGATCCTGATTTTGGTGAATCTGCCATTGGTCGTGTTGCACCAGTTGATTCTGGGTTGTGGTGGATTATTTTATTAAGAGCTTATGGAAAGATTACGGGAGACTACACATTGCAGGAACGTGTCGATGTACAGACAGGCATAAGACTGATCCTTAATCTCTGCTTGACGAATGGGTTCGACATGTTTCCTACTCTGTTAGTTGGTGATGGCTCGTGCATGATTGATAGACGAATGGGCATTCATGGACACCCACTTGAAATTCAAGCATTGTTTTATTCGGCCTTACGCTGCTCCAGAGAGATGCTGATTGTTAACGACTCGACGAAGAATTTGGTGGCTGCCCTGAACAATAGGCTGAGTGCACTCTCCTTCCACATCCGGGAATATTTCTGGGTCGATAAGAACAAACTCAACGAAATTTATCGATATAGAACTGAGGAATATTCTACCGATGCAGTGAACAAGTTCAATATATATCCCGAACAAATTCCTGGTTGGCTGGTGGACTGGATTCCTGAGGAGAGTGGCTACCTGATTGGCAATCTACAGTCTGCTCACATGGACTTCAGGTTTTTTACGCTCGGAAATCTTTGGTCCATTGTTTCGTCACTCGGGACTCCGCAACAGAATGAGGGCATTCTGAACTTGATTGAGGCCAAATGGGATGACCTTGTGGCTAACATGCCTCTCAAGATATGCTTCCCTGCCATGGAACACGAGGAATGGCGCATAATAACTGGAAGCGACCCGAAGAACACTCCTTGGTCATATCATAATGGAGGATCTTGGCCAACTCTCTTGTGGCAGTTCACACTGGCCTGCATGAAGATGGGGCGGCCAGAGCTAGCAAGGAAGGCCATTGCAGTGGCTGAGAAGAAGCTTTCAGCTGATCGTTGGCCCGAGTACTACGACATGCGCAGTGCAAGCTTAATAGGGAAGCAATCACGCCTCTTCCAAACATGGACGATTGCCGGTTTCTTGACATCAAAGTTGCTTCTGGAGAACCCAGAGAAGGCGTCTCTATTGTTCTGGGAGGAGGATTACGAGCTTCTTCAAGGCTGCGTTTGTGTACTCGGCACAGCCAATGGAAACAAGTGCTCTCGCCATCGTCCTGGCCAGCATCGAAAACCGAATAATCTCAACCATTAG

Coding sequence (CDS)

ATGCATACTTCTAGTTCTCTGGGAATTTCTACCATGAAACCTTGTAGGATTCTTTTTAGCTTCAAATCCTCCTCGATGTTTGGAACAACCTCATTGCCCAAGGCTAAGTGTAGGAGGATCGGTAGATTTTCGAAATTAGAGCCGAGTGGGCGTAAAATAGTAGGATCTGTACAGGTTGTTGGTGATTTGAATAGGAGATGCTTTAGTTGTTCTAATTTATATCGGTTGTATAAAGGTAACAGTGGTAGAAATAGGTGTTTGATTGCAAATGTAGCTTCGGATTTTAGAAATCAATCGACATCAGCTGAGCCTCATGTGAAACAGAAGAGCTTTGAGAGAATTTATATTCAAGGAGGGTTTAAGGTTAAGCCATTGGTGATTGAGAGTATTGAGACGGATCTTGTGAAAGATGAAAAAAAGGTGTCTGAGGTTGAGGAACTGAGTGGTTTGAAGGGCTCTAGGGTTGAGAGAGAGGTGTCCAAAATTGAAAAGGAGGCATGGATCTTGCTTCGAGACTCTGTTGTGAACTATTGTGGACATCCTGTTGGAACTGTTGCTGCTAATGATCCATCTGATACTCAGCCACTGAATTACGACCAGGTTTTTGTTCGTGATTTTGTACCGTCCGCCTTGGCCTTCTTGTTAAATGGAGAACAGGAGATTGTCAAGAATTTTCTCCTTCACACCTTGCAATTACAGAGTTGGGAGAAAACCGTTGACTGTTACAGCCCTGGGCAAGGGTTGATGCCAGCAAGTTTCAAAGTTAGAAGCCAACCTCTCGATGGAAACGATGGAGCTTTCGAGGAAGTTCTCGATCCTGATTTTGGTGAATCTGCCATTGGTCGTGTTGCACCAGTTGATTCTGGGTTGTGGTGGATTATTTTATTAAGAGCTTATGGAAAGATTACGGGAGACTACACATTGCAGGAACGTGTCGATGTACAGACAGGCATAAGACTGATCCTTAATCTCTGCTTGACGAATGGGTTCGACATGTTTCCTACTCTGTTAGTTGGTGATGGCTCGTGCATGATTGATAGACGAATGGGCATTCATGGACACCCACTTGAAATTCAAGCATTGTTTTATTCGGCCTTACGCTGCTCCAGAGAGATGCTGATTGTTAACGACTCGACGAAGAATTTGGTGGCTGCCCTGAACAATAGGCTGAGTGCACTCTCCTTCCACATCCGGGAATATTTCTGGGTCGATAAGAACAAACTCAACGAAATTTATCGATATAGAACTGAGGAATATTCTACCGATGCAGTGAACAAGTTCAATATATATCCCGAACAAATTCCTGGTTGGCTGGTGGACTGGATTCCTGAGGAGAGTGGCTACCTGATTGGCAATCTACAGTCTGCTCACATGGACTTCAGGTTTTTTACGCTCGGAAATCTTTGGTCCATTGTTTCGTCACTCGGGACTCCGCAACAGAATGAGGGCATTCTGAACTTGATTGAGGCCAAATGGGATGACCTTGTGGCTAACATGCCTCTCAAGATATGCTTCCCTGCCATGGAACACGAGGAATGGCGCATAATAACTGGAAGCGACCCGAAGAACACTCCTTGGTCATATCATAATGGAGGATCTTGGCCAACTCTCTTGTGGCAGTTCACACTGGCCTGCATGAAGATGGGGCGGCCAGAGCTAGCAAGGAAGGCCATTGCAGTGGCTGAGAAGAAGCTTTCAGCTGATCGTTGGCCCGAGTACTACGACATGCGCAGTGCAAGCTTAATAGGGAAGCAATCACGCCTCTTCCAAACATGGACGATTGCCGGTTTCTTGACATCAAAGTTGCTTCTGGAGAACCCAGAGAAGGCGTCTCTATTGTTCTGGGAGGAGGATTACGAGCTTCTTCAAGGCTGCGTTTGTGTACTCGGCACAGCCAATGGAAACAAGTGCTCTCGCCATCGTCCTGGCCAGCATCGAAAACCGAATAATCTCAACCATTAG

Protein sequence

MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAKCRRIGRFSKLEPSGRKIVGSVQVVGDLNRRCFSCSNLYRLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGFKVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAWILLRDSVVNYCGHPVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYSTDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFRFFTLGNLWSIVSSLGTPQQNEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLFWEEDYELLQGCVCVLGTANGNKCSRHRPGQHRKPNNLNH
Homology
BLAST of CmaCh14G015770 vs. ExPASy Swiss-Prot
Match: B9DFA8 (Alkaline/neutral invertase C, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=INVC PE=1 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 4.4e-259
Identity = 447/667 (67.02%), Postives = 520/667 (77.96%), Query Frame = 0

Query: 1   MHTSSSLGISTMKP-CRILFSFKSSSMFGTTSLPKAKCRRIGRFSKLEPSGRKIVGSV-- 60
           M++ S + +S MKP CR L SF+SSS+FG +     K     +    +   R I   +  
Sbjct: 1   MNSRSCICVSAMKPCCRFLISFRSSSLFGFSPPNSGKFINSSKLHCTKIDSRSIRSGIHC 60

Query: 61  -QVVGDLNRRCFS--------CSNLYRLYKGNSGRNR--CLIANVASDFRNQSTSA-EPH 120
            ++V D N  C S         S + R    + GR R   +I +VASDFRN STS+ + H
Sbjct: 61  RRIVLDRNAFCDSDSISWGGGGSRVLRARGSSRGRGRGVLVIPHVASDFRNYSTSSLDSH 120

Query: 121 VKQKSFERIYIQGGFKVKPLVIESIETDLVKDEKKVSEV----EELSGLKGSRVERE--- 180
           V  KSFE ++      VKPLV + +E      +++   V    +   G  G R E E   
Sbjct: 121 VNDKSFESMF------VKPLVFKEVEKTEGIPKRERGNVGGGKDANFGNVGVRKETERCL 180

Query: 181 -VSKIEKEAWILLRDSVVNYCGHPVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNG 240
             +++EKEAW LLR +VVNYCG PVGTVAANDP DTQ LNYDQVF+RDFVPSA AF+L+G
Sbjct: 181 SQTEVEKEAWKLLRGAVVNYCGFPVGTVAANDPGDTQTLNYDQVFIRDFVPSAYAFMLDG 240

Query: 241 EQEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGE 300
           E EIV+NFLLHTLQLQSWEKTVDC+SPG GLMPASFKV+S PL+GNDG+FEE LDPDFG 
Sbjct: 241 EGEIVRNFLLHTLQLQSWEKTVDCHSPGPGLMPASFKVKSAPLEGNDGSFEEFLDPDFGG 300

Query: 301 SAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLL 360
           SAIGRV+PVDSGLWWIILLRAYGK+TGDYTLQER+DVQTGI+LIL LCL +GFDMFPTLL
Sbjct: 301 SAIGRVSPVDSGLWWIILLRAYGKLTGDYTLQERIDVQTGIKLILKLCLADGFDMFPTLL 360

Query: 361 VGDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHI 420
           V DGSCM+DRRMGIHGHPLEIQALFYSALRC+REMLIVND TK+LV A+NNRLSALSFHI
Sbjct: 361 VTDGSCMVDRRMGIHGHPLEIQALFYSALRCAREMLIVNDGTKSLVTAVNNRLSALSFHI 420

Query: 421 REYFWVDKNKLNEIYRYRTEEYSTDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAH 480
           REY+WVD  K+NEIYRY TEEYS DA NKFNIYPEQIP WLVDWIP++ GY IGNLQ AH
Sbjct: 421 REYYWVDIKKINEIYRYNTEEYSADATNKFNIYPEQIPTWLVDWIPDKGGYFIGNLQPAH 480

Query: 481 MDFRFFTLGNLWSIVSSLGTPQQNEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIIT 540
           MDFRFFTLGNLW+++SSLG  +QNEG++ LIE KWDDLVANMPLKICFPA+E +EWRIIT
Sbjct: 481 MDFRFFTLGNLWAVISSLGNQEQNEGVMTLIEEKWDDLVANMPLKICFPALEKDEWRIIT 540

Query: 541 GSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMR 600
           GSDPKNTPWSYHNGGSWPTLLWQFTLAC+KMG+ ELA+KA+AVAEK+L  D WPEYYD +
Sbjct: 541 GSDPKNTPWSYHNGGSWPTLLWQFTLACIKMGKLELAKKAVAVAEKRLKEDEWPEYYDTK 600

Query: 601 SASLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLFWEEDYELLQGCVCVLGTANG--N 643
           S   +GKQSRL+QTWTIAGFL +K L+E PEKASLLFWEEDY+LL+ CVC L  ++G  N
Sbjct: 601 SGRFVGKQSRLYQTWTIAGFLAAKKLIEQPEKASLLFWEEDYQLLETCVCGLSKSSGRKN 660

BLAST of CmaCh14G015770 vs. ExPASy Swiss-Prot
Match: Q9FXA8 (Alkaline/neutral invertase A, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=INVA PE=1 SV=1)

HSP 1 Score: 872.5 bits (2253), Expect = 3.1e-252
Identity = 426/597 (71.36%), Postives = 479/597 (80.23%), Query Frame = 0

Query: 43  FSKLEPSGRKIVGSVQVVGDLNRRCFSCSNLYRLYKGNSGRNRCLIANVASDFRNQSTSA 102
           FSK  P       S++ +    R  F  S++Y   +     NR          R  STS 
Sbjct: 27  FSKDSPPDLSRTTSIRHLSSSQR--FVSSSIYCFPQSKILPNRFSEKTTGISVRQFSTSV 86

Query: 103 EPHVKQKSFERIYIQGGFKVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKI 162
           E ++  KSFERI++Q             +  L +  K   EVE +S +   +V RE S+ 
Sbjct: 87  ETNLSDKSFERIHVQS------------DAILERIHKNEEEVETVS-IGSEKVVREESEA 146

Query: 163 EKEAWILLRDSVVNYCGHPVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEIV 222
           EKEAW +L ++VV YCG PVGTVAANDP D  PLNYDQVF+RDFVPSALAFLL GE +IV
Sbjct: 147 EKEAWRILENAVVRYCGSPVGTVAANDPGDKMPLNYDQVFIRDFVPSALAFLLKGEGDIV 206

Query: 223 KNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIGR 282
           +NFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVR+  LD N    EEVLDPDFGESAIGR
Sbjct: 207 RNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRTVALDEN--TTEEVLDPDFGESAIGR 266

Query: 283 VAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGS 342
           VAPVDSGLWWIILLRAYGKITGD++LQER+DVQTGI+LI+NLCL +GFDMFPTLLV DGS
Sbjct: 267 VAPVDSGLWWIILLRAYGKITGDFSLQERIDVQTGIKLIMNLCLADGFDMFPTLLVTDGS 326

Query: 343 CMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFW 402
           CMIDRRMGIHGHPLEIQ+LFYSALRCSREML VNDS+K+LV A+NNRLSALSFHIREY+W
Sbjct: 327 CMIDRRMGIHGHPLEIQSLFYSALRCSREMLSVNDSSKDLVRAINNRLSALSFHIREYYW 386

Query: 403 VDKNKLNEIYRYRTEEYSTDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFRF 462
           VD  K+NEIYRY+TEEYSTDA NKFNIYPEQIP WL+DWIPE+ GYL+GNLQ AHMDFRF
Sbjct: 387 VDIKKINEIYRYKTEEYSTDATNKFNIYPEQIPPWLMDWIPEQGGYLLGNLQPAHMDFRF 446

Query: 463 FTLGNLWSIVSSLGTPQQNEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPK 522
           FTLGN WSIVSSL TP+QNE ILNLIEAKWDD++ NMPLKIC+PA+E+++WRIITGSDPK
Sbjct: 447 FTLGNFWSIVSSLATPKQNEAILNLIEAKWDDIIGNMPLKICYPALEYDDWRIITGSDPK 506

Query: 523 NTPWSYHNGGSWPTLLWQFTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLI 582
           NTPWSYHN GSWPTLLWQFTLACMKMGRPELA KA+AVAEK+L ADRWPEYYD RS   I
Sbjct: 507 NTPWSYHNSGSWPTLLWQFTLACMKMGRPELAEKALAVAEKRLLADRWPEYYDTRSGKFI 566

Query: 583 GKQSRLFQTWTIAGFLTSKLLLENPEKASLLFWEEDYELLQGCVCVLGTANGNKCSR 640
           GKQSRL+QTWT+AGFLTSKLLL NPE ASLLFWEEDYELL  C C L  ++  KCSR
Sbjct: 567 GKQSRLYQTWTVAGFLTSKLLLANPEMASLLFWEEDYELLDICACGLRKSDRKKCSR 606

BLAST of CmaCh14G015770 vs. ExPASy Swiss-Prot
Match: Q84JL5 (Probable alkaline/neutral invertase A, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=INVH PE=2 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 3.9e-247
Identity = 410/560 (73.21%), Postives = 469/560 (83.75%), Query Frame = 0

Query: 83  RNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGFKVKPLVIESIETDLVKDEKKVS 142
           R   + A V S+ R+ S S        + ++IY + G  VKPLV+E ++ D  KDE+ V+
Sbjct: 71  RQSSVTAQVVSEARSHSAST-TCANDTTLDQIYTKNGLNVKPLVVERLKRD-EKDEEAVN 130

Query: 143 EVEELSGLKGSRVER-EVSKIEKEAWILLRDSVVNYCGHPVGTVAANDPSDTQPLNYDQV 202
           E EE  G+K    E  + + +E+EAW LLRDS+V YC  PVGTVAA DP+DT P NYDQV
Sbjct: 131 EDEE--GVKRDGFEGVKCNDVEEEAWRLLRDSIVTYCDSPVGTVAAKDPTDTTPSNYDQV 190

Query: 203 FVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLD 262
           F+RDFVPSALAFLL GE EIV+NFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVR+ PL+
Sbjct: 191 FIRDFVPSALAFLLKGESEIVRNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRTLPLE 250

Query: 263 GNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLI 322
             +  FEEVLDPDFGE+AIGRVAPVDSGLWWIILLRAYGKITGDY+LQER+DVQTGI++I
Sbjct: 251 --EDKFEEVLDPDFGEAAIGRVAPVDSGLWWIILLRAYGKITGDYSLQERIDVQTGIKMI 310

Query: 323 LNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDSTKN 382
            NLCL +GFDMFPTLLV DGSCMIDRRMGIHGHPLEIQALFYSALR SREM+ VNDS+KN
Sbjct: 311 ANLCLADGFDMFPTLLVTDGSCMIDRRMGIHGHPLEIQALFYSALRSSREMITVNDSSKN 370

Query: 383 LVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYSTDAVNKFNIYPEQIPGWLVDW 442
           ++  ++NRLSALSFHIRE +WVDKNK+NEIYRY+TEEYS DA NKFNIYPEQ+  WL+DW
Sbjct: 371 IIKTISNRLSALSFHIRENYWVDKNKINEIYRYKTEEYSMDATNKFNIYPEQVSPWLMDW 430

Query: 443 IPE--ESGYLIGNLQSAHMDFRFFTLGNLWSIVSSLGTPQQNEGILNLIEAKWDDLVANM 502
           +PE  +SG+LIGNLQ AHMDFRFFTLGNLWSI+SSLGTP+QN+ ILNL+E KWDDLV +M
Sbjct: 431 VPESPDSGFLIGNLQPAHMDFRFFTLGNLWSIISSLGTPKQNQAILNLVEEKWDDLVGHM 490

Query: 503 PLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARKAIA 562
           PLKIC+PA+E  EW IITGSDPKNTPWSYHNGGSWPTLLWQFTLAC+KMGRPELA KA+ 
Sbjct: 491 PLKICYPALESSEWHIITGSDPKNTPWSYHNGGSWPTLLWQFTLACIKMGRPELAEKAVT 550

Query: 563 VAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLFWEEDY 622
           +AEK+L ADRWPEYYD R    IGKQSRL+QTWTIAGFLTSK LL+NPE AS LFWEED 
Sbjct: 551 LAEKRLQADRWPEYYDTRDGKFIGKQSRLYQTWTIAGFLTSKQLLQNPEIASSLFWEEDL 610

Query: 623 ELLQGCVCVLGTANGNKCSR 640
           ELL+ CVCVL  +   KCSR
Sbjct: 611 ELLESCVCVLTKSGRKKCSR 624

BLAST of CmaCh14G015770 vs. ExPASy Swiss-Prot
Match: Q10MC0 (Neutral/alkaline invertase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=NIN1 PE=1 SV=1)

HSP 1 Score: 825.5 bits (2131), Expect = 4.3e-238
Identity = 393/508 (77.36%), Postives = 440/508 (86.61%), Query Frame = 0

Query: 136 KDEKKVSEVEELSGLKG---SRVEREVSKIEKEAWILLRDSVVNYCGHPVGTVAANDPSD 195
           KD    +   EL GLK    +   R+ S  EKEAW LL  SVV+YCG  VGTVAANDPS 
Sbjct: 112 KDPVATACQHELEGLKAWVETVRSRKESTEEKEAWSLLGRSVVSYCGTAVGTVAANDPST 171

Query: 196 -TQPLNYDQVFVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPA 255
             Q LNYDQVF+RDFVPSA+AFLL GE +IVKNFLLHTLQLQSWEKTVDCYSPGQGLMPA
Sbjct: 172 ANQMLNYDQVFIRDFVPSAIAFLLKGEGDIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPA 231

Query: 256 SFKVRSQPLDGNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQER 315
           SFKVRS PLDGN  AFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDY LQER
Sbjct: 232 SFKVRSIPLDGNSEAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYALQER 291

Query: 316 VDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQALFYSALRCSRE 375
           VDVQTGIRLILNLCL++GFDMFPTLLV DGSCMIDRRMGIHGHPLEIQ+LFYSALRC+RE
Sbjct: 292 VDVQTGIRLILNLCLSDGFDMFPTLLVTDGSCMIDRRMGIHGHPLEIQSLFYSALRCARE 351

Query: 376 MLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYSTDAVNKFNIYP 435
           M+ VND + +L+ A+N RLSALSFHIREY+WVD  K+NEIYRY+TEEYS DA+NKFNIYP
Sbjct: 352 MVSVNDGSNSLIRAINYRLSALSFHIREYYWVDMKKINEIYRYKTEEYSHDAINKFNIYP 411

Query: 436 EQIPGWLVDWIPEESGYLIGNLQSAHMDFRFFTLGNLWSIVSSLGTPQQNEGILNLIEAK 495
           EQIP WL DWIPE+ GYLIGNLQ AHMDFRFF+LGNLW+I+SSL T +Q EGILNLIEAK
Sbjct: 412 EQIPSWLADWIPEKGGYLIGNLQPAHMDFRFFSLGNLWAIISSLATQRQAEGILNLIEAK 471

Query: 496 WDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRP 555
           W+D++ANMPLKIC+PA+E+EEWRIITGSDPKNTPWSYHNGGSWPTLLWQFTLAC+KMGR 
Sbjct: 472 WEDIIANMPLKICYPALEYEEWRIITGSDPKNTPWSYHNGGSWPTLLWQFTLACIKMGRR 531

Query: 556 ELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTSKLLLENPEKAS 615
           +LA++AI VAEK+LS D+WPEYYD R+   IGKQSRL+QTWTIAG+L+SK+LL+ PE AS
Sbjct: 532 DLAQRAIEVAEKRLSEDKWPEYYDTRTGRFIGKQSRLYQTWTIAGYLSSKMLLDCPELAS 591

Query: 616 LLFWEEDYELLQGCVCVLGTANGNKCSR 640
           +L  EED ELL+GC C +  +   KCSR
Sbjct: 592 ILICEEDLELLEGCACSVNKSARTKCSR 619

BLAST of CmaCh14G015770 vs. ExPASy Swiss-Prot
Match: Q6H6N5 (Neutral/alkaline invertase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NIN3 PE=2 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 4.9e-218
Identity = 346/475 (72.84%), Postives = 407/475 (85.68%), Query Frame = 0

Query: 148 SGLKGSRVEREVSKIEKEAWILLRDSVVNYCGHPVGTVAANDPSDTQPLNYDQVFVRDFV 207
           S  K     R+ S +E EAW LLR+SVV YCG PVGT+AANDP+D  P+NYDQVF+RDF+
Sbjct: 112 SAAKPPPQRRKASSVEDEAWELLRESVVYYCGSPVGTIAANDPNDANPMNYDQVFIRDFI 171

Query: 208 PSALAFLLNGEQEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLDGNDGAF 267
           PS +AFLL GE EIV+NF+LHTLQLQSWEKT+DC+SPGQGLMPASFKVR+ PLDG++ A 
Sbjct: 172 PSGIAFLLKGEYEIVRNFILHTLQLQSWEKTMDCHSPGQGLMPASFKVRTIPLDGDEDAT 231

Query: 268 EEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLILNLCLT 327
           EEVLDPDFGE+AIGRVAPVDSGLWWIILLRAYGK +GD T+QER+DVQTGI++IL LCL 
Sbjct: 232 EEVLDPDFGEAAIGRVAPVDSGLWWIILLRAYGKCSGDLTVQERIDVQTGIKMILKLCLA 291

Query: 328 NGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDSTKNLVAALN 387
           +GFDMFPTLLV DGSCMIDRRMGIHGHPLEIQALFYSAL C+REML   D + +L+ ALN
Sbjct: 292 DGFDMFPTLLVTDGSCMIDRRMGIHGHPLEIQALFYSALLCAREMLTPEDGSADLIRALN 351

Query: 388 NRLSALSFHIREYFWVDKNKLNEIYRYRTEEYSTDAVNKFNIYPEQIPGWLVDWIPEESG 447
           NRL ALSFHIREY+WVD  KLNEIYRY+TEEYS DAVNKFNIYP+Q+  WLV+WIP + G
Sbjct: 352 NRLIALSFHIREYYWVDMQKLNEIYRYKTEEYSYDAVNKFNIYPDQVSPWLVEWIPPKGG 411

Query: 448 YLIGNLQSAHMDFRFFTLGNLWSIVSSLGTPQQNEGILNLIEAKWDDLVANMPLKICFPA 507
           Y IGNLQ AHMDFRFF+LGNLWSIVSSL T  Q+  IL+LIE+KW DLVA MPLKIC+PA
Sbjct: 412 YFIGNLQPAHMDFRFFSLGNLWSIVSSLATTHQSHAILDLIESKWSDLVAEMPLKICYPA 471

Query: 508 MEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARKAIAVAEKKLSA 567
           +E++EW+IITGSDPKNTPWSYHNGGSWPTLLWQ T+A +KM RPE+A KA+ VAE++++ 
Sbjct: 472 LENQEWKIITGSDPKNTPWSYHNGGSWPTLLWQLTVASIKMNRPEIAAKAVEVAERRIAI 531

Query: 568 DRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLFWEEDYELL 623
           D+WPEYYD + A  IGKQSRL+QTW+IAG+L +K LL+ P+ A +L  +ED E+L
Sbjct: 532 DKWPEYYDTKRARFIGKQSRLYQTWSIAGYLVAKQLLDKPDAARILSNDEDSEIL 586

BLAST of CmaCh14G015770 vs. ExPASy TrEMBL
Match: A0A6J1IXE1 (Alkaline/neutral invertase OS=Cucurbita maxima OX=3661 GN=LOC111479350 PE=3 SV=1)

HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 653/653 (100.00%), Postives = 653/653 (100.00%), Query Frame = 0

Query: 1   MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAKCRRIGRFSKLEPSGRKIVGSVQVV 60
           MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAKCRRIGRFSKLEPSGRKIVGSVQVV
Sbjct: 1   MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAKCRRIGRFSKLEPSGRKIVGSVQVV 60

Query: 61  GDLNRRCFSCSNLYRLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF 120
           GDLNRRCFSCSNLYRLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF
Sbjct: 61  GDLNRRCFSCSNLYRLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF 120

Query: 121 KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAWILLRDSVVNYCGH 180
           KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAWILLRDSVVNYCGH
Sbjct: 121 KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAWILLRDSVVNYCGH 180

Query: 181 PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSWEKTVD 240
           PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSWEKTVD
Sbjct: 181 PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSWEKTVD 240

Query: 241 CYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG 300
           CYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG
Sbjct: 241 CYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG 300

Query: 301 KITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA 360
           KITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA
Sbjct: 301 KITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA 360

Query: 361 LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS 420
           LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS
Sbjct: 361 LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS 420

Query: 421 TDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFRFFTLGNLWSIVSSLGTPQQ 480
           TDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFRFFTLGNLWSIVSSLGTPQQ
Sbjct: 421 TDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFRFFTLGNLWSIVSSLGTPQQ 480

Query: 481 NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ 540
           NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ
Sbjct: 481 NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ 540

Query: 541 FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS 600
           FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS
Sbjct: 541 FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS 600

Query: 601 KLLLENPEKASLLFWEEDYELLQGCVCVLGTANGNKCSRHRPGQHRKPNNLNH 654
           KLLLENPEKASLLFWEEDYELLQGCVCVLGTANGNKCSRHRPGQHRKPNNLNH
Sbjct: 601 KLLLENPEKASLLFWEEDYELLQGCVCVLGTANGNKCSRHRPGQHRKPNNLNH 653

BLAST of CmaCh14G015770 vs. ExPASy TrEMBL
Match: A0A6J1GVX4 (Alkaline/neutral invertase OS=Cucurbita moschata OX=3662 GN=LOC111457912 PE=3 SV=1)

HSP 1 Score: 1314.7 bits (3401), Expect = 0.0e+00
Identity = 639/653 (97.86%), Postives = 645/653 (98.77%), Query Frame = 0

Query: 1   MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAKCRRIGRFSKLEPSGRKIVGSVQVV 60
           MHTSSSLGISTMKPCRILFSFKSSSMFGTTSL KAK RRIGRFSKLEPSGRKI+GSVQVV
Sbjct: 1   MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLAKAKYRRIGRFSKLEPSGRKIIGSVQVV 60

Query: 61  GDLNRRCFSCSNLYRLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF 120
           GDLNRRCFSCSNL+RLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF
Sbjct: 61  GDLNRRCFSCSNLHRLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF 120

Query: 121 KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAWILLRDSVVNYCGH 180
           KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAW LLRDSVVNYCGH
Sbjct: 121 KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAWNLLRDSVVNYCGH 180

Query: 181 PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSWEKTVD 240
           PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGE+EIVKNFLLHTLQLQSWEKTVD
Sbjct: 181 PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEEEIVKNFLLHTLQLQSWEKTVD 240

Query: 241 CYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG 300
           CYSPGQGLMPASFKVRSQPLDG+DGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG
Sbjct: 241 CYSPGQGLMPASFKVRSQPLDGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG 300

Query: 301 KITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA 360
           KITGDYTLQER+DVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA
Sbjct: 301 KITGDYTLQERIDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA 360

Query: 361 LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS 420
           LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS
Sbjct: 361 LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS 420

Query: 421 TDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFRFFTLGNLWSIVSSLGTPQQ 480
           TDAVNKFNIYPEQIPGWLVDWIPEE GYLIGNLQ AHMDFRFFTLGNLWSIVSSLGTPQQ
Sbjct: 421 TDAVNKFNIYPEQIPGWLVDWIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPQQ 480

Query: 481 NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ 540
           NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ
Sbjct: 481 NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ 540

Query: 541 FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS 600
           FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS
Sbjct: 541 FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS 600

Query: 601 KLLLENPEKASLLFWEEDYELLQGCVCVLGTANGNKCSRHRPGQHRKPNNLNH 654
           KLLL+NPEKASLLFWEEDYELLQGCVCVLG ANGNKCSRHR  QHRKPNNLNH
Sbjct: 601 KLLLDNPEKASLLFWEEDYELLQGCVCVLGKANGNKCSRHRHRQHRKPNNLNH 653

BLAST of CmaCh14G015770 vs. ExPASy TrEMBL
Match: A0A1S3AX88 (Alkaline/neutral invertase OS=Cucumis melo OX=3656 GN=LOC103483905 PE=3 SV=1)

HSP 1 Score: 1191.0 bits (3080), Expect = 0.0e+00
Identity = 583/658 (88.60%), Postives = 610/658 (92.71%), Query Frame = 0

Query: 1   MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAKCRRIGRFSKLEPSGRKIVGSVQVV 60
           MHT SSLGIST+KPCRIL  FKSSSMFGT + PK K +RIGRFSKLEP+G K++GSVQVV
Sbjct: 1   MHTCSSLGISTIKPCRILIGFKSSSMFGTIASPKLKYKRIGRFSKLEPNGCKVIGSVQVV 60

Query: 61  GDLNRRCFSCSNLYRLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF 120
             L+RRCF  SN YRLYKG+S RNR LIANVASDFRNQSTSAE +VKQKSF+ IYI GGF
Sbjct: 61  DKLSRRCFCFSNGYRLYKGSSDRNRHLIANVASDFRNQSTSAESYVKQKSFDAIYINGGF 120

Query: 121 KVKPLVIESIET--DLVKDEKKVSEVEELSGLKG---SRVEREVSKIEKEAWILLRDSVV 180
           KVKPL IESIET  D+VK++KKVSEVE L  LKG   SRVERE+SKIEKEAW LLR+SVV
Sbjct: 121 KVKPLEIESIETGHDIVKEDKKVSEVEGLGSLKGSNYSRVERELSKIEKEAWDLLRNSVV 180

Query: 181 NYCGHPVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSW 240
            YCGHPVGTVAANDP+D QPLNYDQVFVRDFVPSALAFLLNGE+EIVKNFLLHTLQLQSW
Sbjct: 181 FYCGHPVGTVAANDPADAQPLNYDQVFVRDFVPSALAFLLNGEEEIVKNFLLHTLQLQSW 240

Query: 241 EKTVDCYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIIL 300
           EKTVDCYSPGQGLMPASFKVRSQPLDG+DGAFEEVLDPDFGESAIGRVAPVDSGLWWIIL
Sbjct: 241 EKTVDCYSPGQGLMPASFKVRSQPLDGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIIL 300

Query: 301 LRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHP 360
           LRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLV DGSCMIDRRMGIHGHP
Sbjct: 301 LRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHP 360

Query: 361 LEIQALFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYR 420
           LEIQALFYSALRCSREMLIVNDSTKNLV  LNNRLSALSFHIREY+WVDKNK+NEIYRY+
Sbjct: 361 LEIQALFYSALRCSREMLIVNDSTKNLVVELNNRLSALSFHIREYYWVDKNKINEIYRYK 420

Query: 421 TEEYSTDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFRFFTLGNLWSIVSSL 480
           TEEYSTDAVNKFNIYPEQIP WLVDWIPEE GY IGNLQ AHMDFRFFTLGNLWS+VSSL
Sbjct: 421 TEEYSTDAVNKFNIYPEQIPSWLVDWIPEEGGYFIGNLQPAHMDFRFFTLGNLWSVVSSL 480

Query: 481 GTPQQNEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWP 540
           GTP+QNEGILNLIEAKWDDLVANMPLKICFPAME+EEWRIITGSDPKNTPWSYHNGGSWP
Sbjct: 481 GTPKQNEGILNLIEAKWDDLVANMPLKICFPAMEYEEWRIITGSDPKNTPWSYHNGGSWP 540

Query: 541 TLLWQFTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIA 600
           TLLWQFTLAC+KMGRPELAR AIAVAEKKLS DRWPEYYDMRSA LIGKQSRLFQTWTIA
Sbjct: 541 TLLWQFTLACIKMGRPELARNAIAVAEKKLSVDRWPEYYDMRSARLIGKQSRLFQTWTIA 600

Query: 601 GFLTSKLLLENPEKASLLFWEEDYELLQGCVCVLGTANGNKCSRHRPGQHRKPNNLNH 654
           GFLTSKLLLENPEKASLLFWEEDYE+LQ CVC LG  NGNKCSRHR  QH KP+N NH
Sbjct: 601 GFLTSKLLLENPEKASLLFWEEDYEILQNCVCALGKGNGNKCSRHR--QHPKPSNPNH 656

BLAST of CmaCh14G015770 vs. ExPASy TrEMBL
Match: A0A0A0L968 (Alkaline/neutral invertase OS=Cucumis sativus OX=3659 GN=Csa_3G168930 PE=3 SV=1)

HSP 1 Score: 1179.5 bits (3050), Expect = 0.0e+00
Identity = 574/653 (87.90%), Postives = 606/653 (92.80%), Query Frame = 0

Query: 1   MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAKCRRIGRFSKLEPSGRKIVGSVQVV 60
           MHT SSLGISTMKPCRIL  FKSSSMFGT + PK K +RIGRFSKLEP+G KI GSVQVV
Sbjct: 1   MHTCSSLGISTMKPCRILIGFKSSSMFGTIASPKLKYKRIGRFSKLEPNGCKITGSVQVV 60

Query: 61  GDLNRRCFSCSNLYRLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF 120
            +L+RRC   SN YRLYKG++ RNRCLIANVASDFRNQSTS+E +VKQKSF+ IYI GGF
Sbjct: 61  DNLSRRCICFSNGYRLYKGSNDRNRCLIANVASDFRNQSTSSESYVKQKSFDTIYINGGF 120

Query: 121 KVKPLVIESIET--DLVKDEKKVSEVEELSGLKG---SRVEREVSKIEKEAWILLRDSVV 180
           KVKPL IESIET  D+VK++KKVSEVE L  LKG   SRVEREVSKIEKEAW LLR+SVV
Sbjct: 121 KVKPLEIESIETGHDIVKEDKKVSEVEGLGSLKGSNYSRVEREVSKIEKEAWDLLRNSVV 180

Query: 181 NYCGHPVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSW 240
            YCGHPVGTVAANDP+D+QPLNYDQVFVRDF+PSALAFLLNGE+EIVKNFLLHTLQLQSW
Sbjct: 181 FYCGHPVGTVAANDPADSQPLNYDQVFVRDFIPSALAFLLNGEEEIVKNFLLHTLQLQSW 240

Query: 241 EKTVDCYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIIL 300
           EKTVDCYSPGQGLMPASFKVRSQPLDG+DGAFEEVLDPDFGESAIGRVAPVDSGLWWIIL
Sbjct: 241 EKTVDCYSPGQGLMPASFKVRSQPLDGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIIL 300

Query: 301 LRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHP 360
           +RAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLV DGSCMIDRRMGIHGHP
Sbjct: 301 VRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHP 360

Query: 361 LEIQALFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYR 420
           LEIQALFYSALRCSREMLIVNDSTKNLV  LNNRLSALSFHIREY+WVDKNK+NEIYRY+
Sbjct: 361 LEIQALFYSALRCSREMLIVNDSTKNLVVELNNRLSALSFHIREYYWVDKNKINEIYRYK 420

Query: 421 TEEYSTDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFRFFTLGNLWSIVSSL 480
           TEEYS+DAVNKFNIYPEQIP WLVDWIPEE GY +GNLQ AHMDFRFFTLGNLWSIVSSL
Sbjct: 421 TEEYSSDAVNKFNIYPEQIPSWLVDWIPEEGGYFMGNLQPAHMDFRFFTLGNLWSIVSSL 480

Query: 481 GTPQQNEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWP 540
           GTP+QNEGILNLIEAKWDDLVANMPLKICFPAME+EEWRIITGSDPKNTPWSYHNGGSWP
Sbjct: 481 GTPKQNEGILNLIEAKWDDLVANMPLKICFPAMEYEEWRIITGSDPKNTPWSYHNGGSWP 540

Query: 541 TLLWQFTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIA 600
           TLLWQFTLAC+KMGRPE+AR AIAVAEKKLS DRWPEYYDMRSA LIGKQSRLFQTWTIA
Sbjct: 541 TLLWQFTLACIKMGRPEVARNAIAVAEKKLSIDRWPEYYDMRSARLIGKQSRLFQTWTIA 600

Query: 601 GFLTSKLLLENPEKASLLFWEEDYELLQGCVCVLGTANGNKCSRHRPGQHRKP 649
           GFLTSKLLLENPEKASLLFWEEDY++LQ C+C L   NGNKCSRHR  QH KP
Sbjct: 601 GFLTSKLLLENPEKASLLFWEEDYDILQNCICALSKGNGNKCSRHR--QHPKP 651

BLAST of CmaCh14G015770 vs. ExPASy TrEMBL
Match: A0A6J1C8V3 (Alkaline/neutral invertase OS=Momordica charantia OX=3673 GN=LOC111009384 PE=3 SV=1)

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 563/658 (85.56%), Postives = 598/658 (90.88%), Query Frame = 0

Query: 1   MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAKCRRIGRFSKLEPSGRKIVGSVQVV 60
           MHT S LGISTMKPCRIL SFKSSSMFG  S P+ K RRI + SKL P+  +I+GSVQV 
Sbjct: 1   MHTCSCLGISTMKPCRILISFKSSSMFG-ASPPRPKYRRIDKISKLRPNCSRIIGSVQVN 60

Query: 61  GDLNRRCFSCSNLYRLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF 120
            + +RR F  SN YRL +G+ G+NR LIANVASDFRNQSTSAE HV QKSFE IYIQGGF
Sbjct: 61  DNSSRRGFGFSNGYRLSRGSGGKNRHLIANVASDFRNQSTSAEAHVNQKSFETIYIQGGF 120

Query: 121 KVKPLVIESIETD--LVKDEKKVSEVEELSGLKG---SRVEREVSKIEKEAWILLRDSVV 180
            VKPLVIESIET+  L K+EK  SE E L  LK    +RVERE S+IEKEAW LLRDSVV
Sbjct: 121 TVKPLVIESIETNRGLEKEEKSASEAEGLGSLKDFNYARVEREASEIEKEAWSLLRDSVV 180

Query: 181 NYCGHPVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSW 240
           NYCGHPVGTVAANDP+DTQPLNYDQVFVRDFVPSALAFLLNGE EIVKNFLLHTLQLQSW
Sbjct: 181 NYCGHPVGTVAANDPADTQPLNYDQVFVRDFVPSALAFLLNGEDEIVKNFLLHTLQLQSW 240

Query: 241 EKTVDCYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIIL 300
           EKTVDCYSPGQGLMPASFKVRSQPLDG+DGAFEEVLDPDFGESAIGRVAPVDSGLWWIIL
Sbjct: 241 EKTVDCYSPGQGLMPASFKVRSQPLDGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIIL 300

Query: 301 LRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHP 360
           LRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLV DGSCMIDRRMGIHGHP
Sbjct: 301 LRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVSDGSCMIDRRMGIHGHP 360

Query: 361 LEIQALFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYR 420
           LEIQALFYSALRCSREMLIVND+TKNLVAALN+RLS+LSFHIREY+W+D NK+NEIYRY+
Sbjct: 361 LEIQALFYSALRCSREMLIVNDATKNLVAALNSRLSSLSFHIREYYWIDMNKINEIYRYK 420

Query: 421 TEEYSTDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFRFFTLGNLWSIVSSL 480
           TEEYS++AVNKFNIYPEQIP WLVDWIPEE GYLIGNLQ AHMDFRFFTLGNLWSIVSSL
Sbjct: 421 TEEYSSEAVNKFNIYPEQIPSWLVDWIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSL 480

Query: 481 GTPQQNEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWP 540
           GTP+QN+GILNLIEAKWDDLVANMPLKICFPAME+EEWRI+TGSDPKNTPWSYHNGGSWP
Sbjct: 481 GTPKQNKGILNLIEAKWDDLVANMPLKICFPAMEYEEWRIVTGSDPKNTPWSYHNGGSWP 540

Query: 541 TLLWQFTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIA 600
           TLLWQFTLACMKMGRPELAR AIAVAEKKLSADRWPEYYDMRSA LIGKQSRLFQTWTIA
Sbjct: 541 TLLWQFTLACMKMGRPELARNAIAVAEKKLSADRWPEYYDMRSARLIGKQSRLFQTWTIA 600

Query: 601 GFLTSKLLLENPEKASLLFWEEDYELLQGCVCVLGTANGNKCSRHRPGQHRKPNNLNH 654
           GFLTSKLLLENPEKASLL+WEEDYELLQ CVC LG ANGN+CSR R     +P++ +H
Sbjct: 601 GFLTSKLLLENPEKASLLYWEEDYELLQSCVCALGKANGNRCSRQR--HPARPDDHDH 655

BLAST of CmaCh14G015770 vs. NCBI nr
Match: XP_022979724.1 (alkaline/neutral invertase A, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 653/653 (100.00%), Postives = 653/653 (100.00%), Query Frame = 0

Query: 1   MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAKCRRIGRFSKLEPSGRKIVGSVQVV 60
           MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAKCRRIGRFSKLEPSGRKIVGSVQVV
Sbjct: 1   MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAKCRRIGRFSKLEPSGRKIVGSVQVV 60

Query: 61  GDLNRRCFSCSNLYRLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF 120
           GDLNRRCFSCSNLYRLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF
Sbjct: 61  GDLNRRCFSCSNLYRLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF 120

Query: 121 KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAWILLRDSVVNYCGH 180
           KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAWILLRDSVVNYCGH
Sbjct: 121 KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAWILLRDSVVNYCGH 180

Query: 181 PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSWEKTVD 240
           PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSWEKTVD
Sbjct: 181 PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSWEKTVD 240

Query: 241 CYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG 300
           CYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG
Sbjct: 241 CYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG 300

Query: 301 KITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA 360
           KITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA
Sbjct: 301 KITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA 360

Query: 361 LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS 420
           LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS
Sbjct: 361 LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS 420

Query: 421 TDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFRFFTLGNLWSIVSSLGTPQQ 480
           TDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFRFFTLGNLWSIVSSLGTPQQ
Sbjct: 421 TDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFRFFTLGNLWSIVSSLGTPQQ 480

Query: 481 NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ 540
           NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ
Sbjct: 481 NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ 540

Query: 541 FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS 600
           FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS
Sbjct: 541 FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS 600

Query: 601 KLLLENPEKASLLFWEEDYELLQGCVCVLGTANGNKCSRHRPGQHRKPNNLNH 654
           KLLLENPEKASLLFWEEDYELLQGCVCVLGTANGNKCSRHRPGQHRKPNNLNH
Sbjct: 601 KLLLENPEKASLLFWEEDYELLQGCVCVLGTANGNKCSRHRPGQHRKPNNLNH 653

BLAST of CmaCh14G015770 vs. NCBI nr
Match: XP_022956128.1 (alkaline/neutral invertase A, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 1314.7 bits (3401), Expect = 0.0e+00
Identity = 639/653 (97.86%), Postives = 645/653 (98.77%), Query Frame = 0

Query: 1   MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAKCRRIGRFSKLEPSGRKIVGSVQVV 60
           MHTSSSLGISTMKPCRILFSFKSSSMFGTTSL KAK RRIGRFSKLEPSGRKI+GSVQVV
Sbjct: 1   MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLAKAKYRRIGRFSKLEPSGRKIIGSVQVV 60

Query: 61  GDLNRRCFSCSNLYRLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF 120
           GDLNRRCFSCSNL+RLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF
Sbjct: 61  GDLNRRCFSCSNLHRLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF 120

Query: 121 KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAWILLRDSVVNYCGH 180
           KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAW LLRDSVVNYCGH
Sbjct: 121 KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAWNLLRDSVVNYCGH 180

Query: 181 PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSWEKTVD 240
           PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGE+EIVKNFLLHTLQLQSWEKTVD
Sbjct: 181 PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEEEIVKNFLLHTLQLQSWEKTVD 240

Query: 241 CYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG 300
           CYSPGQGLMPASFKVRSQPLDG+DGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG
Sbjct: 241 CYSPGQGLMPASFKVRSQPLDGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG 300

Query: 301 KITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA 360
           KITGDYTLQER+DVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA
Sbjct: 301 KITGDYTLQERIDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA 360

Query: 361 LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS 420
           LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS
Sbjct: 361 LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS 420

Query: 421 TDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFRFFTLGNLWSIVSSLGTPQQ 480
           TDAVNKFNIYPEQIPGWLVDWIPEE GYLIGNLQ AHMDFRFFTLGNLWSIVSSLGTPQQ
Sbjct: 421 TDAVNKFNIYPEQIPGWLVDWIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPQQ 480

Query: 481 NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ 540
           NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ
Sbjct: 481 NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ 540

Query: 541 FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS 600
           FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS
Sbjct: 541 FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS 600

Query: 601 KLLLENPEKASLLFWEEDYELLQGCVCVLGTANGNKCSRHRPGQHRKPNNLNH 654
           KLLL+NPEKASLLFWEEDYELLQGCVCVLG ANGNKCSRHR  QHRKPNNLNH
Sbjct: 601 KLLLDNPEKASLLFWEEDYELLQGCVCVLGKANGNKCSRHRHRQHRKPNNLNH 653

BLAST of CmaCh14G015770 vs. NCBI nr
Match: KAG7018557.1 (Alkaline/neutral invertase C, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1311.6 bits (3393), Expect = 0.0e+00
Identity = 638/653 (97.70%), Postives = 644/653 (98.62%), Query Frame = 0

Query: 1   MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAKCRRIGRFSKLEPSGRKIVGSVQVV 60
           MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAK RRIGRFSKLEPSGRKI+GSVQVV
Sbjct: 1   MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAKYRRIGRFSKLEPSGRKIIGSVQVV 60

Query: 61  GDLNRRCFSCSNLYRLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF 120
           GDLN+RCFSCSNL+RLYKGNSGRNRCLIANVASDFRNQSTSAEP VKQKSFERIYIQGGF
Sbjct: 61  GDLNKRCFSCSNLHRLYKGNSGRNRCLIANVASDFRNQSTSAEPRVKQKSFERIYIQGGF 120

Query: 121 KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAWILLRDSVVNYCGH 180
           KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAW LLRDSVVNYCG 
Sbjct: 121 KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAWNLLRDSVVNYCGR 180

Query: 181 PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSWEKTVD 240
           PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGE+EIVKNFLLHTLQLQSWEKTVD
Sbjct: 181 PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEEEIVKNFLLHTLQLQSWEKTVD 240

Query: 241 CYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG 300
           CYSPGQGLMPASFKVRSQPLDG+DGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG
Sbjct: 241 CYSPGQGLMPASFKVRSQPLDGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG 300

Query: 301 KITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA 360
           KITGDYTLQER+DVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA
Sbjct: 301 KITGDYTLQERIDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA 360

Query: 361 LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS 420
           LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS
Sbjct: 361 LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS 420

Query: 421 TDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFRFFTLGNLWSIVSSLGTPQQ 480
           TDAVNKFNIYPEQIPGWLVDWIPEE GYLIGNLQ AHMDFRFFTLGNLWSIVSSLGTPQQ
Sbjct: 421 TDAVNKFNIYPEQIPGWLVDWIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPQQ 480

Query: 481 NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ 540
           NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ
Sbjct: 481 NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ 540

Query: 541 FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS 600
           FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS
Sbjct: 541 FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS 600

Query: 601 KLLLENPEKASLLFWEEDYELLQGCVCVLGTANGNKCSRHRPGQHRKPNNLNH 654
           KLLLENPEKASLLFWEEDYELLQGCVCVLG ANGNKCSRHR  QHRKPNNLNH
Sbjct: 601 KLLLENPEKASLLFWEEDYELLQGCVCVLGKANGNKCSRHRHRQHRKPNNLNH 653

BLAST of CmaCh14G015770 vs. NCBI nr
Match: XP_023528476.1 (alkaline/neutral invertase A, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1308.9 bits (3386), Expect = 0.0e+00
Identity = 637/653 (97.55%), Postives = 643/653 (98.47%), Query Frame = 0

Query: 1   MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAKCRRIGRFSKLEPSGRKIVGSVQVV 60
           MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAK RRIGRFSKLEPSGRKI+GSVQVV
Sbjct: 1   MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAKYRRIGRFSKLEPSGRKIIGSVQVV 60

Query: 61  GDLNRRCFSCSNLYRLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF 120
           GDLNRRCFSCSNL+RLYKGNSGRNR LIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF
Sbjct: 61  GDLNRRCFSCSNLHRLYKGNSGRNRFLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF 120

Query: 121 KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAWILLRDSVVNYCGH 180
           KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAW LLRDSVVNYCG 
Sbjct: 121 KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAWNLLRDSVVNYCGR 180

Query: 181 PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSWEKTVD 240
           PVGTVA NDPSDTQPLNYDQVFVRDFVPSALAFLLNGE+EIVKNFLLHTLQLQSWEKTVD
Sbjct: 181 PVGTVATNDPSDTQPLNYDQVFVRDFVPSALAFLLNGEEEIVKNFLLHTLQLQSWEKTVD 240

Query: 241 CYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG 300
           CYSPGQGLMPASFKVRSQPLDG+DGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG
Sbjct: 241 CYSPGQGLMPASFKVRSQPLDGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG 300

Query: 301 KITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA 360
           KITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA
Sbjct: 301 KITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA 360

Query: 361 LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS 420
           LFYSALRCSREMLIVNDSTKNLVAA+NNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS
Sbjct: 361 LFYSALRCSREMLIVNDSTKNLVAAMNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS 420

Query: 421 TDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFRFFTLGNLWSIVSSLGTPQQ 480
           TDAVNKFNIYPEQIPGWLVDWIPEE GYLIGNLQ AHMDFRFFTLGNLWSIVSSLGTPQQ
Sbjct: 421 TDAVNKFNIYPEQIPGWLVDWIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPQQ 480

Query: 481 NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ 540
           NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ
Sbjct: 481 NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ 540

Query: 541 FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS 600
           FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS
Sbjct: 541 FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS 600

Query: 601 KLLLENPEKASLLFWEEDYELLQGCVCVLGTANGNKCSRHRPGQHRKPNNLNH 654
           KLLLENPEKASLLFWEEDYE+LQGCVCVLG ANGNKCSRHR  QHRKPNNLNH
Sbjct: 601 KLLLENPEKASLLFWEEDYEILQGCVCVLGKANGNKCSRHRHRQHRKPNNLNH 653

BLAST of CmaCh14G015770 vs. NCBI nr
Match: KAG6582157.1 (Alkaline/neutral invertase C, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1305.0 bits (3376), Expect = 0.0e+00
Identity = 635/653 (97.24%), Postives = 642/653 (98.32%), Query Frame = 0

Query: 1   MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAKCRRIGRFSKLEPSGRKIVGSVQVV 60
           MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAK RRIGRFSKLEPSGRKI+GSVQVV
Sbjct: 1   MHTSSSLGISTMKPCRILFSFKSSSMFGTTSLPKAKYRRIGRFSKLEPSGRKIIGSVQVV 60

Query: 61  GDLNRRCFSCSNLYRLYKGNSGRNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGF 120
           GDLN+RCFSCSNL+RLYKGNSGRNRCLIANVASDFRNQSTSAEP VKQKSFERIYIQGGF
Sbjct: 61  GDLNKRCFSCSNLHRLYKGNSGRNRCLIANVASDFRNQSTSAEPRVKQKSFERIYIQGGF 120

Query: 121 KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAWILLRDSVVNYCGH 180
           KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAW LLRDSVVNYCG 
Sbjct: 121 KVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKIEKEAWNLLRDSVVNYCGR 180

Query: 181 PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSWEKTVD 240
           PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGE+EIVKNFLLHTLQLQSWEKTVD
Sbjct: 181 PVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEEEIVKNFLLHTLQLQSWEKTVD 240

Query: 241 CYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG 300
           CYSPGQGLMPASFKVRSQPLDG+DGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG
Sbjct: 241 CYSPGQGLMPASFKVRSQPLDGSDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYG 300

Query: 301 KITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA 360
           KITGDYTLQER+DVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA
Sbjct: 301 KITGDYTLQERIDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQA 360

Query: 361 LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS 420
           LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS
Sbjct: 361 LFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYS 420

Query: 421 TDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFRFFTLGNLWSIVSSLGTPQQ 480
           TDAVNKFNIYPEQIPGWLVDWIPEE GYLIGNLQ AHMDFRFFTLGNLWSIVSSLGTPQQ
Sbjct: 421 TDAVNKFNIYPEQIPGWLVDWIPEEGGYLIGNLQPAHMDFRFFTLGNLWSIVSSLGTPQQ 480

Query: 481 NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQ 540
           NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWR+ITGSDPKNTPWSYHNGGSWPTLLWQ
Sbjct: 481 NEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRVITGSDPKNTPWSYHNGGSWPTLLWQ 540

Query: 541 FTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS 600
           FTLACMKMGRPELARKAIAVAEKKLSAD WPEYYDMRSASLIGKQSRLFQTWTIAGFLTS
Sbjct: 541 FTLACMKMGRPELARKAIAVAEKKLSADGWPEYYDMRSASLIGKQSRLFQTWTIAGFLTS 600

Query: 601 KLLLENPEKASLLFWEEDYELLQGCVCVLGTANGNKCSRHRPGQHRKPNNLNH 654
           KLLLENPEKASLLFWEEDYELLQGCVCVLG ANGNKCSR R  QHRKPNNLNH
Sbjct: 601 KLLLENPEKASLLFWEEDYELLQGCVCVLGKANGNKCSRDRHRQHRKPNNLNH 653

BLAST of CmaCh14G015770 vs. TAIR 10
Match: AT3G06500.1 (Plant neutral invertase family protein )

HSP 1 Score: 895.2 bits (2312), Expect = 3.1e-260
Identity = 447/667 (67.02%), Postives = 520/667 (77.96%), Query Frame = 0

Query: 1   MHTSSSLGISTMKP-CRILFSFKSSSMFGTTSLPKAKCRRIGRFSKLEPSGRKIVGSV-- 60
           M++ S + +S MKP CR L SF+SSS+FG +     K     +    +   R I   +  
Sbjct: 1   MNSRSCICVSAMKPCCRFLISFRSSSLFGFSPPNSGKFINSSKLHCTKIDSRSIRSGIHC 60

Query: 61  -QVVGDLNRRCFS--------CSNLYRLYKGNSGRNR--CLIANVASDFRNQSTSA-EPH 120
            ++V D N  C S         S + R    + GR R   +I +VASDFRN STS+ + H
Sbjct: 61  RRIVLDRNAFCDSDSISWGGGGSRVLRARGSSRGRGRGVLVIPHVASDFRNYSTSSLDSH 120

Query: 121 VKQKSFERIYIQGGFKVKPLVIESIETDLVKDEKKVSEV----EELSGLKGSRVERE--- 180
           V  KSFE ++      VKPLV + +E      +++   V    +   G  G R E E   
Sbjct: 121 VNDKSFESMF------VKPLVFKEVEKTEGIPKRERGNVGGGKDANFGNVGVRKETERCL 180

Query: 181 -VSKIEKEAWILLRDSVVNYCGHPVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNG 240
             +++EKEAW LLR +VVNYCG PVGTVAANDP DTQ LNYDQVF+RDFVPSA AF+L+G
Sbjct: 181 SQTEVEKEAWKLLRGAVVNYCGFPVGTVAANDPGDTQTLNYDQVFIRDFVPSAYAFMLDG 240

Query: 241 EQEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGE 300
           E EIV+NFLLHTLQLQSWEKTVDC+SPG GLMPASFKV+S PL+GNDG+FEE LDPDFG 
Sbjct: 241 EGEIVRNFLLHTLQLQSWEKTVDCHSPGPGLMPASFKVKSAPLEGNDGSFEEFLDPDFGG 300

Query: 301 SAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLL 360
           SAIGRV+PVDSGLWWIILLRAYGK+TGDYTLQER+DVQTGI+LIL LCL +GFDMFPTLL
Sbjct: 301 SAIGRVSPVDSGLWWIILLRAYGKLTGDYTLQERIDVQTGIKLILKLCLADGFDMFPTLL 360

Query: 361 VGDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHI 420
           V DGSCM+DRRMGIHGHPLEIQALFYSALRC+REMLIVND TK+LV A+NNRLSALSFHI
Sbjct: 361 VTDGSCMVDRRMGIHGHPLEIQALFYSALRCAREMLIVNDGTKSLVTAVNNRLSALSFHI 420

Query: 421 REYFWVDKNKLNEIYRYRTEEYSTDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAH 480
           REY+WVD  K+NEIYRY TEEYS DA NKFNIYPEQIP WLVDWIP++ GY IGNLQ AH
Sbjct: 421 REYYWVDIKKINEIYRYNTEEYSADATNKFNIYPEQIPTWLVDWIPDKGGYFIGNLQPAH 480

Query: 481 MDFRFFTLGNLWSIVSSLGTPQQNEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIIT 540
           MDFRFFTLGNLW+++SSLG  +QNEG++ LIE KWDDLVANMPLKICFPA+E +EWRIIT
Sbjct: 481 MDFRFFTLGNLWAVISSLGNQEQNEGVMTLIEEKWDDLVANMPLKICFPALEKDEWRIIT 540

Query: 541 GSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMR 600
           GSDPKNTPWSYHNGGSWPTLLWQFTLAC+KMG+ ELA+KA+AVAEK+L  D WPEYYD +
Sbjct: 541 GSDPKNTPWSYHNGGSWPTLLWQFTLACIKMGKLELAKKAVAVAEKRLKEDEWPEYYDTK 600

Query: 601 SASLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLFWEEDYELLQGCVCVLGTANG--N 643
           S   +GKQSRL+QTWTIAGFL +K L+E PEKASLLFWEEDY+LL+ CVC L  ++G  N
Sbjct: 601 SGRFVGKQSRLYQTWTIAGFLAAKKLIEQPEKASLLFWEEDYQLLETCVCGLSKSSGRKN 660

BLAST of CmaCh14G015770 vs. TAIR 10
Match: AT1G56560.1 (Plant neutral invertase family protein )

HSP 1 Score: 872.5 bits (2253), Expect = 2.2e-253
Identity = 426/597 (71.36%), Postives = 479/597 (80.23%), Query Frame = 0

Query: 43  FSKLEPSGRKIVGSVQVVGDLNRRCFSCSNLYRLYKGNSGRNRCLIANVASDFRNQSTSA 102
           FSK  P       S++ +    R  F  S++Y   +     NR          R  STS 
Sbjct: 27  FSKDSPPDLSRTTSIRHLSSSQR--FVSSSIYCFPQSKILPNRFSEKTTGISVRQFSTSV 86

Query: 103 EPHVKQKSFERIYIQGGFKVKPLVIESIETDLVKDEKKVSEVEELSGLKGSRVEREVSKI 162
           E ++  KSFERI++Q             +  L +  K   EVE +S +   +V RE S+ 
Sbjct: 87  ETNLSDKSFERIHVQS------------DAILERIHKNEEEVETVS-IGSEKVVREESEA 146

Query: 163 EKEAWILLRDSVVNYCGHPVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEIV 222
           EKEAW +L ++VV YCG PVGTVAANDP D  PLNYDQVF+RDFVPSALAFLL GE +IV
Sbjct: 147 EKEAWRILENAVVRYCGSPVGTVAANDPGDKMPLNYDQVFIRDFVPSALAFLLKGEGDIV 206

Query: 223 KNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIGR 282
           +NFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVR+  LD N    EEVLDPDFGESAIGR
Sbjct: 207 RNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRTVALDEN--TTEEVLDPDFGESAIGR 266

Query: 283 VAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGS 342
           VAPVDSGLWWIILLRAYGKITGD++LQER+DVQTGI+LI+NLCL +GFDMFPTLLV DGS
Sbjct: 267 VAPVDSGLWWIILLRAYGKITGDFSLQERIDVQTGIKLIMNLCLADGFDMFPTLLVTDGS 326

Query: 343 CMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFW 402
           CMIDRRMGIHGHPLEIQ+LFYSALRCSREML VNDS+K+LV A+NNRLSALSFHIREY+W
Sbjct: 327 CMIDRRMGIHGHPLEIQSLFYSALRCSREMLSVNDSSKDLVRAINNRLSALSFHIREYYW 386

Query: 403 VDKNKLNEIYRYRTEEYSTDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFRF 462
           VD  K+NEIYRY+TEEYSTDA NKFNIYPEQIP WL+DWIPE+ GYL+GNLQ AHMDFRF
Sbjct: 387 VDIKKINEIYRYKTEEYSTDATNKFNIYPEQIPPWLMDWIPEQGGYLLGNLQPAHMDFRF 446

Query: 463 FTLGNLWSIVSSLGTPQQNEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPK 522
           FTLGN WSIVSSL TP+QNE ILNLIEAKWDD++ NMPLKIC+PA+E+++WRIITGSDPK
Sbjct: 447 FTLGNFWSIVSSLATPKQNEAILNLIEAKWDDIIGNMPLKICYPALEYDDWRIITGSDPK 506

Query: 523 NTPWSYHNGGSWPTLLWQFTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLI 582
           NTPWSYHN GSWPTLLWQFTLACMKMGRPELA KA+AVAEK+L ADRWPEYYD RS   I
Sbjct: 507 NTPWSYHNSGSWPTLLWQFTLACMKMGRPELAEKALAVAEKRLLADRWPEYYDTRSGKFI 566

Query: 583 GKQSRLFQTWTIAGFLTSKLLLENPEKASLLFWEEDYELLQGCVCVLGTANGNKCSR 640
           GKQSRL+QTWT+AGFLTSKLLL NPE ASLLFWEEDYELL  C C L  ++  KCSR
Sbjct: 567 GKQSRLYQTWTVAGFLTSKLLLANPEMASLLFWEEDYELLDICACGLRKSDRKKCSR 606

BLAST of CmaCh14G015770 vs. TAIR 10
Match: AT3G05820.1 (invertase H )

HSP 1 Score: 855.5 bits (2209), Expect = 2.8e-248
Identity = 410/560 (73.21%), Postives = 469/560 (83.75%), Query Frame = 0

Query: 83  RNRCLIANVASDFRNQSTSAEPHVKQKSFERIYIQGGFKVKPLVIESIETDLVKDEKKVS 142
           R   + A V S+ R+ S S        + ++IY + G  VKPLV+E ++ D  KDE+ V+
Sbjct: 97  RQSSVTAQVVSEARSHSAST-TCANDTTLDQIYTKNGLNVKPLVVERLKRD-EKDEEAVN 156

Query: 143 EVEELSGLKGSRVER-EVSKIEKEAWILLRDSVVNYCGHPVGTVAANDPSDTQPLNYDQV 202
           E EE  G+K    E  + + +E+EAW LLRDS+V YC  PVGTVAA DP+DT P NYDQV
Sbjct: 157 EDEE--GVKRDGFEGVKCNDVEEEAWRLLRDSIVTYCDSPVGTVAAKDPTDTTPSNYDQV 216

Query: 203 FVRDFVPSALAFLLNGEQEIVKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLD 262
           F+RDFVPSALAFLL GE EIV+NFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVR+ PL+
Sbjct: 217 FIRDFVPSALAFLLKGESEIVRNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRTLPLE 276

Query: 263 GNDGAFEEVLDPDFGESAIGRVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLI 322
             +  FEEVLDPDFGE+AIGRVAPVDSGLWWIILLRAYGKITGDY+LQER+DVQTGI++I
Sbjct: 277 --EDKFEEVLDPDFGEAAIGRVAPVDSGLWWIILLRAYGKITGDYSLQERIDVQTGIKMI 336

Query: 323 LNLCLTNGFDMFPTLLVGDGSCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDSTKN 382
            NLCL +GFDMFPTLLV DGSCMIDRRMGIHGHPLEIQALFYSALR SREM+ VNDS+KN
Sbjct: 337 ANLCLADGFDMFPTLLVTDGSCMIDRRMGIHGHPLEIQALFYSALRSSREMITVNDSSKN 396

Query: 383 LVAALNNRLSALSFHIREYFWVDKNKLNEIYRYRTEEYSTDAVNKFNIYPEQIPGWLVDW 442
           ++  ++NRLSALSFHIRE +WVDKNK+NEIYRY+TEEYS DA NKFNIYPEQ+  WL+DW
Sbjct: 397 IIKTISNRLSALSFHIRENYWVDKNKINEIYRYKTEEYSMDATNKFNIYPEQVSPWLMDW 456

Query: 443 IPE--ESGYLIGNLQSAHMDFRFFTLGNLWSIVSSLGTPQQNEGILNLIEAKWDDLVANM 502
           +PE  +SG+LIGNLQ AHMDFRFFTLGNLWSI+SSLGTP+QN+ ILNL+E KWDDLV +M
Sbjct: 457 VPESPDSGFLIGNLQPAHMDFRFFTLGNLWSIISSLGTPKQNQAILNLVEEKWDDLVGHM 516

Query: 503 PLKICFPAMEHEEWRIITGSDPKNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARKAIA 562
           PLKIC+PA+E  EW IITGSDPKNTPWSYHNGGSWPTLLWQFTLAC+KMGRPELA KA+ 
Sbjct: 517 PLKICYPALESSEWHIITGSDPKNTPWSYHNGGSWPTLLWQFTLACIKMGRPELAEKAVT 576

Query: 563 VAEKKLSADRWPEYYDMRSASLIGKQSRLFQTWTIAGFLTSKLLLENPEKASLLFWEEDY 622
           +AEK+L ADRWPEYYD R    IGKQSRL+QTWTIAGFLTSK LL+NPE AS LFWEED 
Sbjct: 577 LAEKRLQADRWPEYYDTRDGKFIGKQSRLYQTWTIAGFLTSKQLLQNPEIASSLFWEEDL 636

Query: 623 ELLQGCVCVLGTANGNKCSR 640
           ELL+ CVCVL  +   KCSR
Sbjct: 637 ELLESCVCVLTKSGRKKCSR 650

BLAST of CmaCh14G015770 vs. TAIR 10
Match: AT5G22510.1 (alkaline/neutral invertase )

HSP 1 Score: 749.2 bits (1933), Expect = 2.8e-216
Identity = 343/468 (73.29%), Postives = 400/468 (85.47%), Query Frame = 0

Query: 162 IEKEAWILLRDSVVNYCGHPVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEI 221
           IE EAW LLR SVV YCG P+GT+AANDP+ T  LNYDQVF+RDF+PS +AFLL GE +I
Sbjct: 131 IEDEAWDLLRQSVVFYCGSPIGTIAANDPNSTSVLNYDQVFIRDFIPSGIAFLLKGEYDI 190

Query: 222 VKNFLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIG 281
           V+NF+L+TLQLQSWEKT+DC+SPGQGLMP SFKV++ PLDG+D   EEVLDPDFGE+AIG
Sbjct: 191 VRNFILYTLQLQSWEKTMDCHSPGQGLMPCSFKVKTVPLDGDDSMTEEVLDPDFGEAAIG 250

Query: 282 RVAPVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDG 341
           RVAPVDSGLWWIILLRAYGK TGD ++QERVDVQTGI++IL LCL +GFDMFPTLLV DG
Sbjct: 251 RVAPVDSGLWWIILLRAYGKCTGDLSVQERVDVQTGIKMILKLCLADGFDMFPTLLVTDG 310

Query: 342 SCMIDRRMGIHGHPLEIQALFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYF 401
           SCMIDRRMGIHGHPLEIQALFYSAL C+REML   D + +L+ ALNNRL AL+FHIREY+
Sbjct: 311 SCMIDRRMGIHGHPLEIQALFYSALVCAREMLTPEDGSADLIRALNNRLVALNFHIREYY 370

Query: 402 WVDKNKLNEIYRYRTEEYSTDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFR 461
           W+D  K+NEIYRY+TEEYS DAVNKFNIYP+QIP WLVD++P   GYLIGNLQ AHMDFR
Sbjct: 371 WLDLKKINEIYRYQTEEYSYDAVNKFNIYPDQIPSWLVDFMPNRGGYLIGNLQPAHMDFR 430

Query: 462 FFTLGNLWSIVSSLGTPQQNEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDP 521
           FFTLGNLWSIVSSL +  Q+  IL+ IEAKW +LVA+MPLKIC+PAME EEWRIITGSDP
Sbjct: 431 FFTLGNLWSIVSSLASNDQSHAILDFIEAKWAELVADMPLKICYPAMEGEEWRIITGSDP 490

Query: 522 KNTPWSYHNGGSWPTLLWQFTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASL 581
           KNTPWSYHNGG+WPTLLWQ T+A +KMGRPE+A KA+ +AE+++S D+WPEYYD + A  
Sbjct: 491 KNTPWSYHNGGAWPTLLWQLTVASIKMGRPEIAEKAVELAERRISLDKWPEYYDTKRARF 550

Query: 582 IGKQSRLFQTWTIAGFLTSKLLLENPEKASLLFWEEDYELLQGCVCVL 630
           IGKQ+RL+QTW+IAG+L +KLLL NP  A  L  EED +L     C+L
Sbjct: 551 IGKQARLYQTWSIAGYLVAKLLLANPAAAKFLTSEEDSDLRNAFSCML 598

BLAST of CmaCh14G015770 vs. TAIR 10
Match: AT4G34860.1 (Plant neutral invertase family protein )

HSP 1 Score: 585.1 bits (1507), Expect = 6.9e-167
Identity = 271/457 (59.30%), Postives = 347/457 (75.93%), Query Frame = 0

Query: 165 EAWILLRDSVVNYCGHPVGTVAANDPSDTQPLNYDQVFVRDFVPSALAFLLNGEQEIVKN 224
           EAW  LR S+V + G PVGT+AA D S+ + LNYDQVFVRDFVPSALAFL+NGE +IVKN
Sbjct: 109 EAWDALRRSMVYFRGQPVGTIAAVDNSE-EKLNYDQVFVRDFVPSALAFLMNGEPDIVKN 168

Query: 225 FLLHTLQLQSWEKTVDCYSPGQGLMPASFKVRSQPLDGNDGAFEEVLDPDFGESAIGRVA 284
           FLL TL+LQSWEK +D +  G+G+MPASFKV   P+  +     E L  DFGESAIGRVA
Sbjct: 169 FLLKTLRLQSWEKKIDRFQLGEGVMPASFKVFHDPVRNH-----ETLIADFGESAIGRVA 228

Query: 285 PVDSGLWWIILLRAYGKITGDYTLQERVDVQTGIRLILNLCLTNGFDMFPTLLVGDGSCM 344
           PVDSG WWIILLRAY K TGD +L +  + Q GIRLIL+LCL+ GFD FPTLL  DG CM
Sbjct: 229 PVDSGFWWIILLRAYTKSTGDSSLADMPECQKGIRLILSLCLSEGFDTFPTLLCADGCCM 288

Query: 345 IDRRMGIHGHPLEIQALFYSALRCSREMLIVNDSTKNLVAALNNRLSALSFHIREYFWVD 404
           IDRRMG++G+P+EIQALF+ ALRC+  +L  +   K +V  +  RL ALS+H+R YFW+D
Sbjct: 289 IDRRMGVYGYPIEIQALFFMALRCALLLLKHDGEGKEMVEQIVKRLHALSYHMRSYFWLD 348

Query: 405 KNKLNEIYRYRTEEYSTDAVNKFNIYPEQIPGWLVDWIPEESGYLIGNLQSAHMDFRFFT 464
             +LN+IYRY+TEEYS  AVNKFN+ P+ +P W+ D++P   G+ IGN+  A MDFR+F 
Sbjct: 349 LKQLNDIYRYKTEEYSHTAVNKFNVIPDSLPEWVFDFMPPHGGFFIGNVSPARMDFRWFA 408

Query: 465 LGNLWSIVSSLGTPQQNEGILNLIEAKWDDLVANMPLKICFPAMEHEEWRIITGSDPKNT 524
           LGN  +I+SSL TP+Q+  I++LIE++W++LV  MPLK+C+PA+E  EWRI+TG DPKNT
Sbjct: 409 LGNCIAILSSLATPEQSTAIMDLIESRWEELVGEMPLKVCYPAIESHEWRIVTGCDPKNT 468

Query: 525 PWSYHNGGSWPTLLWQFTLACMKMGRPELARKAIAVAEKKLSADRWPEYYDMRSASLIGK 584
            WSYHNGGSWP LLW  T AC+K GRP++AR+AI VAE +L  D WPEYYD +    +GK
Sbjct: 469 RWSYHNGGSWPVLLWLLTAACIKTGRPQIARRAIEVAEARLHKDHWPEYYDGKVGRYVGK 528

Query: 585 QSRLFQTWTIAGFLTSKLLLENPEKASLLFWEEDYEL 622
           QSR  QTW++AG+L +K++LE+P    ++  EED ++
Sbjct: 529 QSRKNQTWSVAGYLVAKMMLEDPSHVGMVCLEEDKQM 559

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B9DFA84.4e-25967.02Alkaline/neutral invertase C, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=I... [more]
Q9FXA83.1e-25271.36Alkaline/neutral invertase A, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=I... [more]
Q84JL53.9e-24773.21Probable alkaline/neutral invertase A, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q10MC04.3e-23877.36Neutral/alkaline invertase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=3... [more]
Q6H6N54.9e-21872.84Neutral/alkaline invertase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1IXE10.0e+00100.00Alkaline/neutral invertase OS=Cucurbita maxima OX=3661 GN=LOC111479350 PE=3 SV=1[more]
A0A6J1GVX40.0e+0097.86Alkaline/neutral invertase OS=Cucurbita moschata OX=3662 GN=LOC111457912 PE=3 SV... [more]
A0A1S3AX880.0e+0088.60Alkaline/neutral invertase OS=Cucumis melo OX=3656 GN=LOC103483905 PE=3 SV=1[more]
A0A0A0L9680.0e+0087.90Alkaline/neutral invertase OS=Cucumis sativus OX=3659 GN=Csa_3G168930 PE=3 SV=1[more]
A0A6J1C8V30.0e+0085.56Alkaline/neutral invertase OS=Momordica charantia OX=3673 GN=LOC111009384 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_022979724.10.0e+00100.00alkaline/neutral invertase A, mitochondrial-like [Cucurbita maxima][more]
XP_022956128.10.0e+0097.86alkaline/neutral invertase A, mitochondrial-like [Cucurbita moschata][more]
KAG7018557.10.0e+0097.70Alkaline/neutral invertase C, mitochondrial [Cucurbita argyrosperma subsp. argyr... [more]
XP_023528476.10.0e+0097.55alkaline/neutral invertase A, mitochondrial-like [Cucurbita pepo subsp. pepo][more]
KAG6582157.10.0e+0097.24Alkaline/neutral invertase C, mitochondrial, partial [Cucurbita argyrosperma sub... [more]
Match NameE-valueIdentityDescription
AT3G06500.13.1e-26067.02Plant neutral invertase family protein [more]
AT1G56560.12.2e-25371.36Plant neutral invertase family protein [more]
AT3G05820.12.8e-24873.21invertase H [more]
AT5G22510.12.8e-21673.29alkaline/neutral invertase [more]
AT4G34860.16.9e-16759.30Plant neutral invertase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 127..147
NoneNo IPR availablePANTHERPTHR31916:SF49ALKALINE/NEUTRAL INVERTASE C, MITOCHONDRIALcoord: 6..641
IPR024746Glycosyl hydrolase family 100PFAMPF12899Glyco_hydro_100coord: 166..607
e-value: 6.4E-213
score: 707.2
IPR024746Glycosyl hydrolase family 100PANTHERPTHR31916FAMILY NOT NAMEDcoord: 6..641
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 193..597
e-value: 6.5E-16
score: 60.2
IPR008928Six-hairpin glycosidase superfamilySUPERFAMILY48208Six-hairpin glycosidasescoord: 164..610

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh14G015770.1CmaCh14G015770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005987 sucrose catabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005739 mitochondrion
molecular_function GO:0033926 glycopeptide alpha-N-acetylgalactosaminidase activity
molecular_function GO:0004575 sucrose alpha-glucosidase activity