CmaCh14G015310 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh14G015310
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	protein RALF-like 32
Location	Cma_Chr14: 11587261 .. 11587605 (+)
RNA-Seq Expression	CmaCh14G015310
Synteny	CmaCh14G015310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGGAAGCTCAAGCCATTCAAGCCTCAACCTCGTCCTCGGCCTTCTATATCTATTGATTTTTACTAACGTCGTCGTTGTCGTCGCAGTCGATAGCTCAATAAGCCTGTGCAATGGTTCGATAGCGGAGTGCTATAAAGAGGTTGAGACGTTGATGGAGTCGGAGATAACTCGGAGGTTTCTTGGACAAGTTAATGAATTAACCTATAAATTTTTGGATAAAGACAAAGCACCTTGTGCTAATGGTAGAGGTAAACCTTACTTTGTAAAGTGTCAGCCTCGTGAAGCTAATCATTATGACAGGAGATGCAGCGGAACGTATAATCGATGTAAATTTTCATAG

mRNA sequence

ATGGAAGGAAGCTCAAGCCATTCAAGCCTCAACCTCGTCCTCGGCCTTCTATATCTATTGATTTTTACTAACGTCGTCGTTGTCGTCGCAGTCGATAGCTCAATAAGCCTGTGCAATGGTTCGATAGCGGAGTGCTATAAAGAGGTTGAGACGTTGATGGAGTCGGAGATAACTCGGAGGTTTCTTGGACAAGTTAATGAATTAACCTATAAATTTTTGGATAAAGACAAAGCACCTTGTGCTAATGGTAGAGGTAAACCTTACTTTGTAAAGTGTCAGCCTCGTGAAGCTAATCATTATGACAGGAGATGCAGCGGAACGTATAATCGATGTAAATTTTCATAG

Coding sequence (CDS)

ATGGAAGGAAGCTCAAGCCATTCAAGCCTCAACCTCGTCCTCGGCCTTCTATATCTATTGATTTTTACTAACGTCGTCGTTGTCGTCGCAGTCGATAGCTCAATAAGCCTGTGCAATGGTTCGATAGCGGAGTGCTATAAAGAGGTTGAGACGTTGATGGAGTCGGAGATAACTCGGAGGTTTCTTGGACAAGTTAATGAATTAACCTATAAATTTTTGGATAAAGACAAAGCACCTTGTGCTAATGGTAGAGGTAAACCTTACTTTGTAAAGTGTCAGCCTCGTGAAGCTAATCATTATGACAGGAGATGCAGCGGAACGTATAATCGATGTAAATTTTCATAG

Protein sequence

MEGSSSHSSLNLVLGLLYLLIFTNVVVVVAVDSSISLCNGSIAECYKEVETLMESEITRRFLGQVNELTYKFLDKDKAPCANGRGKPYFVKCQPREANHYDRRCSGTYNRCKFS

Homology

[Close]

BLAST of CmaCh14G015310 vs. ExPASy Swiss-Prot
Match: O23262 (Protein RALF-like 32 OS=Arabidopsis thaliana OX=3702 GN=RALFL32 PE=3 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.2e-07
Identity = 38/91 (41.76%), Postives = 52/91 (57.14%), Query Frame = 0


Query: 28  VVAVDSSISLCNGSIAECYKEVET-----LMESEITRRFL-GQVNELTYKFLDKDKAPCA 87
           V +  SS SLCNGS+AEC   VET     +MES  ++R    Q ++L+Y  L +++  C 
Sbjct: 24  VTSKASSSSLCNGSVAECSSMVETEEMSVIMESWSSQRLTEEQAHKLSYGALRRNQPACD 83

Query: 88  NG-RGKPYFVKCQPREANHYDRRCSGTYNRC 112
            G RG+ Y  +C P  +N Y R CS  Y RC
Sbjct: 84  GGKRGESYSTQCLPPPSNPYSRGCSKHY-RC 113

[Close]

BLAST of CmaCh14G015310 vs. ExPASy Swiss-Prot
Match: Q9SRY3 (Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.4e-06
Identity = 28/68 (41.18%), Postives = 38/68 (55.88%), Query Frame = 0


Query: 38  CNGSIAECYKEVETLMESEITRRFLGQVNELTYKFLDKDKAPCANGRGKPYFVKCQPREA 97
           C+GSIAEC    E  M+SEI RR L     ++Y+ L ++  PC+  RG  Y+      +A
Sbjct: 47  CHGSIAECIGAEEEEMDSEINRRILATTKYISYQSLKRNSVPCSR-RGASYYNCQNGAQA 106

Query: 98  NHYDRRCS 106
           N Y R CS
Sbjct: 107 NPYSRGCS 113

[Close]

BLAST of CmaCh14G015310 vs. ExPASy Swiss-Prot
Match: Q9MA62 (Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.4e-06
Identity = 32/81 (39.51%), Postives = 46/81 (56.79%), Query Frame = 0


Query: 33  SSISLCNGSIAECYKEVETL-MESEITRRFLGQVNELTYKFLDKDKAPCANGRGKPYFVK 92
           S  S C GSIAEC  E E +  +S+I+RR L Q   ++Y  + ++  PC+  RG  Y+  
Sbjct: 40  SLFSGCTGSIAECIAEEEEMEFDSDISRRILAQKKYISYGAMRRNSVPCSR-RGASYYNC 99

Query: 93  CQPREANHYDRRCSGTYNRCK 113
            +  +AN Y R CS T  RC+
Sbjct: 100 QRGAQANPYSRGCS-TITRCR 118

[Close]

BLAST of CmaCh14G015310 vs. ExPASy TrEMBL
Match: A0A6J1C8Z5 (protein RALF-like 32 OS=Momordica charantia OX=3673 GN=LOC111009300 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 4.6e-13
Identity = 53/104 (50.96%), Postives = 65/104 (62.50%), Query Frame = 0


Query: 12  LVLGLLYLLIFTNVVVVVAVDSSISLCNGSIAECYKEVETLMESEITRRFLGQVNE-LTY 71
           L+L LL L  F NV  V+   +S++ CNGSIAECY E E LMESEI+RRFL Q  + ++Y
Sbjct: 20  LILVLLCLPSFNNVFTVLG--NSMTPCNGSIAECYVEEEMLMESEISRRFLEQQKKYISY 79

Query: 72  KFLDKDKAPCANGRGKPY--FVKCQPREANHYDRRCSGTYNRCK 113
             L KD+  C  G+GKPY     C P  +N YDR C   Y RCK
Sbjct: 80  DTLKKDQPACQGGKGKPYTKSESCLPPPSNPYDRGCKPIY-RCK 120

[Close]

BLAST of CmaCh14G015310 vs. ExPASy TrEMBL
Match: A0A6J1C986 (protein RALF-like 32 OS=Momordica charantia OX=3673 GN=LOC111009478 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.0e-12
Identity = 50/97 (51.55%), Postives = 61/97 (62.89%), Query Frame = 0


Query: 19  LLIFTNVVVVVAVDSSISLCNGSIAECYKEVETLMESEITRRFLGQVNE-LTYKFLDKDK 78
           LL F N V +V   SS++ CNGSI+ECY E ETL ESEI+RRFL Q  + ++Y    KD+
Sbjct: 21  LLSFNNAVTIVG--SSMTPCNGSISECYAEEETLXESEISRRFLEQQKKYISYDARKKDQ 80

Query: 79  APCANGRGKPY--FVKCQPREANHYDRRCSGTYNRCK 113
             C  G+GKPY     C P  +N YDR CS  Y RCK
Sbjct: 81  PACKGGKGKPYTESESCLPPPSNPYDRGCSLIY-RCK 114

[Close]

BLAST of CmaCh14G015310 vs. ExPASy TrEMBL
Match: A0A1U7ZHZ9 (protein RALF-like 32 OS=Nelumbo nucifera OX=4432 GN=LOC104590362 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 5.0e-12
Identity = 51/116 (43.97%), Postives = 66/116 (56.90%), Query Frame = 0


Query: 1   MEGSSSHSSLNLVLGLLYL--LIFTNVVVVVAVDSS--ISLCNGSIAECYKEVETLMESE 60
           M  SS+ SSL  +L  L L  L+++  V V A +SS     CNGSIAEC +E E LMESE
Sbjct: 39  MAASSNRSSLGFILATLLLLQLLYSGCVRVSAAESSERYRQCNGSIAECSEEEEVLMESE 98

Query: 61  ITRRFLGQVNELTYKFLDKDKAPCANGRGKPYFVKCQPREANHYDRRCSGTYNRCK 113
           I+RRFL Q N + Y  L  ++  C   +G  Y   C P  +N  +R CS  Y RC+
Sbjct: 99  ISRRFLAQSNSIGYGALKPNQPACGGPKGTSYSGSCLPNPSNPQNRGCSKIY-RCR 153

[Close]

BLAST of CmaCh14G015310 vs. ExPASy TrEMBL
Match: A0A4S4E196 (Uncharacterized protein OS=Camellia sinensis var. sinensis OX=542762 GN=TEA_021728 PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 9.5e-11
Identity = 45/100 (45.00%), Postives = 61/100 (61.00%), Query Frame = 0


Query: 14  LGLLYLLIFTNVVVVVAVDSSISLCNGSIAECYKEVETLMESEITRRFLGQVNELTYKFL 73
           L LL LL     V V   D+ +  CNGSIAEC+ ++E LMESE++RRFL   N +TY  L
Sbjct: 143 LVLLSLLCLGGCVGVTTSDAHVQ-CNGSIAECHADMEMLMESEVSRRFLETTNHITYGAL 202

Query: 74  DKDKAPCANGR-GKPYFVKCQPREANHYDRRCSGTYNRCK 113
            +D+  C +G+ G+ Y   C P+E+N   R CS  Y RC+
Sbjct: 203 KQDQPACNSGKSGEAYSKSCLPKESNPESRGCS-EYYRCR 240

[Close]

BLAST of CmaCh14G015310 vs. ExPASy TrEMBL
Match: A0A484MYX9 (Uncharacterized protein OS=Cuscuta campestris OX=132261 GN=CCAM_LOCUS35453 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 4.7e-10
Identity = 38/79 (48.10%), Postives = 53/79 (67.09%), Query Frame = 0


Query: 36  SLCNGSIAEC-YKEVETLMESEITRRFLGQVNELTYKFLDKDKAPC-ANGRGKPYFVKCQ 95
           SLCNGSIA+C   + E L+ESE++RRFL ++  +T   L +D+  C + G GKPY   C 
Sbjct: 10  SLCNGSIADCNIVDTELLLESEVSRRFLEEMKHITPGALKRDEPVCYSGGPGKPYTRSCV 69

Query: 96  PREANHYDRRCSGTYNRCK 113
           P  ++HYDR CS +Y RC+
Sbjct: 70  PEPSHHYDRGCS-SYYRCR 87

[Close]

BLAST of CmaCh14G015310 vs. NCBI nr
Match: KAG6582109.1 (Protein RALF-like 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018526.1 Protein RALF-like 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 198.0 bits (502), Expect = 4.5e-47
Identity = 99/112 (88.39%), Postives = 104/112 (92.86%), Query Frame = 0


Query: 1   MEGSSSHSSLNLVLGLLYLLIFTNVVVVVAVDSSISLCNGSIAECYKEVETLMESEITRR 60
           MEGSSSHSSLNLVLGLLYLLIFTNVVVVVAVDS IS CNGSIAECYKEVETLMESEI+RR
Sbjct: 1   MEGSSSHSSLNLVLGLLYLLIFTNVVVVVAVDSLISPCNGSIAECYKEVETLMESEISRR 60

Query: 61  FLGQVNELTYKFLDKDKAPCANGRGKPYFVKCQPREANHYDRRCSGTYNRCK 113
           FLG+  +LTY+FLDK+KAPCANGRGKPY  KCQPREAN YDR CSGTYNRCK
Sbjct: 61  FLGRPKDLTYEFLDKNKAPCANGRGKPYRGKCQPREANPYDRECSGTYNRCK 112

[Close]

BLAST of CmaCh14G015310 vs. NCBI nr
Match: XP_022138044.1 (protein RALF-like 32 [Momordica charantia])

HSP 1 Score: 84.0 bits (206), Expect = 9.4e-13
Identity = 53/104 (50.96%), Postives = 65/104 (62.50%), Query Frame = 0


Query: 12  LVLGLLYLLIFTNVVVVVAVDSSISLCNGSIAECYKEVETLMESEITRRFLGQVNE-LTY 71
           L+L LL L  F NV  V+   +S++ CNGSIAECY E E LMESEI+RRFL Q  + ++Y
Sbjct: 20  LILVLLCLPSFNNVFTVLG--NSMTPCNGSIAECYVEEEMLMESEISRRFLEQQKKYISY 79

Query: 72  KFLDKDKAPCANGRGKPY--FVKCQPREANHYDRRCSGTYNRCK 113
             L KD+  C  G+GKPY     C P  +N YDR C   Y RCK
Sbjct: 80  DTLKKDQPACQGGKGKPYTKSESCLPPPSNPYDRGCKPIY-RCK 120

[Close]

BLAST of CmaCh14G015310 vs. NCBI nr
Match: XP_022138261.1 (protein RALF-like 32 [Momordica charantia])

HSP 1 Score: 82.8 bits (203), Expect = 2.1e-12
Identity = 50/97 (51.55%), Postives = 61/97 (62.89%), Query Frame = 0


Query: 19  LLIFTNVVVVVAVDSSISLCNGSIAECYKEVETLMESEITRRFLGQVNE-LTYKFLDKDK 78
           LL F N V +V   SS++ CNGSI+ECY E ETL ESEI+RRFL Q  + ++Y    KD+
Sbjct: 21  LLSFNNAVTIVG--SSMTPCNGSISECYAEEETLXESEISRRFLEQQKKYISYDARKKDQ 80

Query: 79  APCANGRGKPY--FVKCQPREANHYDRRCSGTYNRCK 113
             C  G+GKPY     C P  +N YDR CS  Y RCK
Sbjct: 81  PACKGGKGKPYTESESCLPPPSNPYDRGCSLIY-RCK 114

[Close]

BLAST of CmaCh14G015310 vs. NCBI nr
Match: DAD24133.1 (TPA_asm: hypothetical protein HUJ06_025596 [Nelumbo nucifera])

HSP 1 Score: 80.5 bits (197), Expect = 1.0e-11
Identity = 51/116 (43.97%), Postives = 66/116 (56.90%), Query Frame = 0


Query: 1   MEGSSSHSSLNLVLGLLYL--LIFTNVVVVVAVDSS--ISLCNGSIAECYKEVETLMESE 60
           M  SS+ SSL  +L  L L  L+++  V V A +SS     CNGSIAEC +E E LMESE
Sbjct: 8   MAASSNRSSLGFILATLLLLQLLYSGCVRVSAAESSERYRQCNGSIAECSEEEEVLMESE 67

Query: 61  ITRRFLGQVNELTYKFLDKDKAPCANGRGKPYFVKCQPREANHYDRRCSGTYNRCK 113
           I+RRFL Q N + Y  L  ++  C   +G  Y   C P  +N  +R CS  Y RC+
Sbjct: 68  ISRRFLAQSNSIGYGALKPNQPACGGPKGTSYSGSCLPNPSNPQNRGCSKIY-RCR 122

[Close]

BLAST of CmaCh14G015310 vs. NCBI nr
Match: XP_010247297.1 (PREDICTED: protein RALF-like 32 [Nelumbo nucifera])

HSP 1 Score: 80.5 bits (197), Expect = 1.0e-11
Identity = 51/116 (43.97%), Postives = 66/116 (56.90%), Query Frame = 0


Query: 1   MEGSSSHSSLNLVLGLLYL--LIFTNVVVVVAVDSS--ISLCNGSIAECYKEVETLMESE 60
           M  SS+ SSL  +L  L L  L+++  V V A +SS     CNGSIAEC +E E LMESE
Sbjct: 39  MAASSNRSSLGFILATLLLLQLLYSGCVRVSAAESSERYRQCNGSIAECSEEEEVLMESE 98

Query: 61  ITRRFLGQVNELTYKFLDKDKAPCANGRGKPYFVKCQPREANHYDRRCSGTYNRCK 113
           I+RRFL Q N + Y  L  ++  C   +G  Y   C P  +N  +R CS  Y RC+
Sbjct: 99  ISRRFLAQSNSIGYGALKPNQPACGGPKGTSYSGSCLPNPSNPQNRGCSKIY-RCR 153

[Close]

BLAST of CmaCh14G015310 vs. TAIR 10
Match: AT4G14010.1 (ralf-like 32 )

HSP 1 Score: 57.4 bits (137), Expect = 8.8e-09
Identity = 38/91 (41.76%), Postives = 52/91 (57.14%), Query Frame = 0


Query: 28  VVAVDSSISLCNGSIAECYKEVET-----LMESEITRRFL-GQVNELTYKFLDKDKAPCA 87
           V +  SS SLCNGS+AEC   VET     +MES  ++R    Q ++L+Y  L +++  C 
Sbjct: 24  VTSKASSSSLCNGSVAECSSMVETEEMSVIMESWSSQRLTEEQAHKLSYGALRRNQPACD 83

Query: 88  NG-RGKPYFVKCQPREANHYDRRCSGTYNRC 112
            G RG+ Y  +C P  +N Y R CS  Y RC
Sbjct: 84  GGKRGESYSTQCLPPPSNPYSRGCSKHY-RC 113

[Close]

BLAST of CmaCh14G015310 vs. TAIR 10
Match: AT1G02900.1 (rapid alkalinization factor 1 )

HSP 1 Score: 53.9 bits (128), Expect = 9.7e-08
Identity = 28/68 (41.18%), Postives = 38/68 (55.88%), Query Frame = 0


Query: 38  CNGSIAECYKEVETLMESEITRRFLGQVNELTYKFLDKDKAPCANGRGKPYFVKCQPREA 97
           C+GSIAEC    E  M+SEI RR L     ++Y+ L ++  PC+  RG  Y+      +A
Sbjct: 47  CHGSIAECIGAEEEEMDSEINRRILATTKYISYQSLKRNSVPCSR-RGASYYNCQNGAQA 106

Query: 98  NHYDRRCS 106
           N Y R CS
Sbjct: 107 NPYSRGCS 113

[Close]

BLAST of CmaCh14G015310 vs. TAIR 10
Match: AT3G05490.1 (ralf-like 22 )

HSP 1 Score: 53.9 bits (128), Expect = 9.7e-08
Identity = 32/81 (39.51%), Postives = 46/81 (56.79%), Query Frame = 0


Query: 33  SSISLCNGSIAECYKEVETL-MESEITRRFLGQVNELTYKFLDKDKAPCANGRGKPYFVK 92
           S  S C GSIAEC  E E +  +S+I+RR L Q   ++Y  + ++  PC+  RG  Y+  
Sbjct: 40  SLFSGCTGSIAECIAEEEEMEFDSDISRRILAQKKYISYGAMRRNSVPCSR-RGASYYNC 99

Query: 93  CQPREANHYDRRCSGTYNRCK 113
            +  +AN Y R CS T  RC+
Sbjct: 100 QRGAQANPYSRGCS-TITRCR 118

The following BLAST results are available for this feature:

BLAST of CmaCh14G015310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23262	1.2e-07	41.76	Protein RALF-like 32 OS=Arabidopsis thaliana OX=3702 GN=RALFL32 PE=3 SV=1	[more]
Q9SRY3	1.4e-06	41.18	Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1	[more]
Q9MA62	1.4e-06	39.51	Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1	[more]

BLAST of CmaCh14G015310 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C8Z5	4.6e-13	50.96	protein RALF-like 32 OS=Momordica charantia OX=3673 GN=LOC111009300 PE=3 SV=1	[more]
A0A6J1C986	1.0e-12	51.55	protein RALF-like 32 OS=Momordica charantia OX=3673 GN=LOC111009478 PE=3 SV=1	[more]
A0A1U7ZHZ9	5.0e-12	43.97	protein RALF-like 32 OS=Nelumbo nucifera OX=4432 GN=LOC104590362 PE=3 SV=1	[more]
A0A4S4E196	9.5e-11	45.00	Uncharacterized protein OS=Camellia sinensis var. sinensis OX=542762 GN=TEA_0217...	[more]
A0A484MYX9	4.7e-10	48.10	Uncharacterized protein OS=Cuscuta campestris OX=132261 GN=CCAM_LOCUS35453 PE=3 ...	[more]

BLAST of CmaCh14G015310 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6582109.1	4.5e-47	88.39	Protein RALF-like 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018526...	[more]
XP_022138044.1	9.4e-13	50.96	protein RALF-like 32 [Momordica charantia]	[more]
XP_022138261.1	2.1e-12	51.55	protein RALF-like 32 [Momordica charantia]	[more]
DAD24133.1	1.0e-11	43.97	TPA_asm: hypothetical protein HUJ06_025596 [Nelumbo nucifera]	[more]
XP_010247297.1	1.0e-11	43.97	PREDICTED: protein RALF-like 32 [Nelumbo nucifera]	[more]

BLAST of CmaCh14G015310 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14010.1	8.8e-09	41.76	ralf-like 32	[more]
AT1G02900.1	9.7e-08	41.18	rapid alkalinization factor 1	[more]
AT3G05490.1	9.7e-08	39.51	ralf-like 22	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 50..112 e-value: 6.0E-13 score: 49.6
None	No IPR available	PANTHER	PTHR33136:SF4	PROTEIN RALF-LIKE 32	coord: 9..112
None	No IPR available	PANTHER	PTHR33136	RAPID ALKALINIZATION FACTOR-LIKE	coord: 9..112

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh14G015310.1	CmaCh14G015310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0110165	cellular anatomical entity