CmaCh14G014790 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh14G014790
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionProlyl-tRNA synthetase
LocationCma_Chr14: 11243385 .. 11248688 (+)
RNA-Seq ExpressionCmaCh14G014790
SyntenyCmaCh14G014790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGGTCCTAAGCCTGGTAGCTCTGCCACTAAACCAAAAGCTGGTAAATGATGTTCCTTTAATACAGCAGTTGTTTGTGCAATTGTGTCCTATTCCCTTGTTTGGCTTGTGTTTACTTGTTCTTTGTTGTTCACTGGGCAGGCGGGAAAAAGGAGGTGAAGAAAGAGACTGGGTTGGGTCTCACCAATAAGAAGGACGACAACTTCGGAGAATGGTATTCCGAGGTGCTGTTTTGTGCACGGTTTTTGTTTTCTTCATTGCAAAATGACTCTTTATGTTTGGTAAAAATTATACGTATGAGTTTGAGAATGTTGTTTGACAGTCGTCGTAGTGAAGGTAGATCGTGTAATTTCATTCGAAGGCTGTCAACGAACATGTCTCAACCCATGTGTGAAATTATTCGTATGAGTCGACCTTTACTTCTCGATGAAGGAGCAAACAAAAAGGAAAAAAAAAGATTATAAGATGTCTTTTTCGAGGGATCTCCCCTGTAATATTATGAACACGTTCTTTTCGATGACTTGAATAATTCTTTTTCTAGTTTGTCTAGGTCGTTGTCAGTGGGGAAATGATCGAGTATTATGATATCTCGGGCTGCTATATACTGAGGCCGTGGGCGATGTCTATCTGGGAGACTATGCAAGTAAGGATGCTAGTCTTTCTATCGTCGATTTTACGATCTGTTCTTGTTTCGAAGTTCAAAGATTTAATTTGTCATCGGTGCAGGTATTTTTTGATGCTGAAATTAAGAAAATGAAAATCAAGAATTGCTATTTTCCACTTTTTGTATCTCCCGGCGTCTTACAAAGAGAGAAAGATCATATAGAAGGTTTTGCTCCTGAGGTGATGTTTTATTATTCATTTATGCAACGGTAGAGAAACTTCTCCATCTGAATTTTAACTTCGCAATAACTCGGGATTTAATCTCATAGCGATGATAAATGTTTCGCTTGTAAATTTAATGGGCTGCATTGCATCTTGGTACTTTCAACTTCATGATTGTATTCCAGGCTGAGCACGACATCCTTATGCACCCATTCCACATGTTAGGTGTAGCTGGTGTATTCGGCCCTCCCTATTCAGTGCTATGCATGGTTTCTTGCTAACTTTTAGTTTGATTAGGGAAACCACAGAAAATGAATCTGCTAATGAAGGTTATAGATTCAGTCAAGAGGAAGAAACTTATAATTTCGTTGCTGCTCAGGGTTATTTTTGCCAATTGGTCTTCCAATATGCTAGTTTCAACAACTCTCGTTCTTTACATTTCTTCCATTTTGTTTTTTAAAATTGATTAGAAATGGGTATTTCATTTTTAATGAAGTTTAAATGCATATTACCTTTGTTATGGGTGTAATACCAGTCAAGTGATGGTATGGTTGTCATGGTCATTGGGTTATGATTTACGAATATCATCTCGATCTTCATGATGTTTCGTTTATGTCGAGTGTCTCTGAGTGAATTGGTTTTATGTCCTCAGTTTGTTCTTCTAGCCTCTAACCTGTTTCTTGTCAGCATAGTTTTGGCTCGAGTGATGAACTTTTATTGCCTCCTAGTAAAAAACATTCTTGAAAAGATTTTAGACATCCTCCCTCCTCCCATTTTGTAATAGCCCAAGCCCACTGCTGGTAGATATTGTTTGCTTTGGCCCGTTACGTATCGCCGTCAGCTCACGGTTTTAAAACACGTATGCTAGGGAGAGGTTTCTACACCCTTACAAGGAATATATCGTTCCCTTCTCCAACCAACGTGGGATCTCATACCCTTTTACCAGTTTAGTAAGAATTTATTCGATGATGAGTAATCTTTCTTAGAATTTTATGTTTGTTTCTAAGAAGTCGTTCCATTCGTTATGTTCGGTTCATTCATTTTTAATTTTTAAGTTTATAAAGGTGGAATTGTGGTTTAGTGCTGACCATTTGGTACTTGGCATCCATTTGAAGGTTGCTTGGGTAACAAAATCTGGGGAGTCTGACTTGGAAGTGCCCATTGCGATTCGTCCTACAAGTGAGACTGTTATGTACCCGTACTATTCGAAGTGGATCAGGGGTCACCGTGACTTGCCTTTGAAACTTAACCAGTGGTGCAATGTTGTGAGATGGGAATTCAGCCATCCCACACCATTTATTAGGCAATTACTCGTGCTTGTGCTTAAGTTTAAATTACTGTATTGTTTTGAAAGCAAAGACTAATTTTCTTTAACTATCGACTGCAGGAGTCGAGAGTTCCTTTGGCAGGAAGGTCATACTGCGTTTGCCACTAAGGATGAAGCAGATGCGGAGGTATAAAACTCTAGAATTTTAAATTATGATTTAGAAACGTTTTTATCTCGAGCAAATTTGAAGATTCAATGTGAGATCCCACATTGGTTGGAGAGGGGAACGAAACATTCTTTATAAGGGTGTCGAAACCTCTCCCTAGCTAGCAGACACGTTTTAAAATTCTTGTGGGAAAACTCGGAAGAGAGAGCCCAAAGAGGATGACAATATCTGCTAGCGGTGGGCTTGGGCTATTACGAATGGTATAAGAGTCAGACACAGGGTGGTGTGCTAGCAAGGACACTAAGCCCTGAAGGAGGGTGGACAACGGGCAGTGTGCCAGTGAGGACATTGGGCTTCGAAGGGGGGTGGACACCGGGCGGTGTGCCAGCAAGGATGCTGGATCCTGAAGGGGGTGGATTATGAGGTCCCACATCGATTGGAGAGCGGAACAAGTGCCAGCGAAGACGCTGGGCCCTGAAGGGGGTGGATTGTGGGATTCCATATCGGTTGGAGAGGGAAACAAAGCATTATGTATATCGGTTGGAGAGGGAAAAAAAGTCTTTTTTATAAGAGTGTGGAAACTTCTCCCTAGCAGACGCGTTTTAAAAACCTTGAGGGAAAACCGAAAGAGGACAATATCTGCTAGGTGTGGGCTTGGACTGTTACTTTCAAATATGCGAACTCAACTCACTTCGATTGGAACCACAAAGAATGAGTGGGGTCTAAATTGGGAGCACCAAGTTCTTCAAGTGATCATCCTTGAGTGCTTTGAACCCAAGGATGAGTTTAAAGGGAGAATTCCAAAACATCCTTCAATCGAACCCTGAAATATTCAGTTATTTTATGAATCTGTGGTATTTTAAAGGTTACTTTTATTTCAGGTTCTTGAAATATTGGAATTGTATAGGCGGATATACGAAGAATACCTGGCCATTCCTGTTATCAAGGGAAAGAAGAGCGAGATGGAGAAGTTTGCCGGTGGTCTTTACACCACGAGCGTGGAGGTATAAGTTGATTAAATGTTATGACCTGTTTTCAGCACGGTGAATCGATACCCGGTTCTTGTTTATGCTGTATGAAACTGTTTCTTCTATGCAGGCATTTATCCCGAACACTGGTCGTGGAATTCAGGGTGCGACTTCACATTGCTTGGGTCAAAATTTCGCAAAAATGTTCGAAATCAACTTTGAAAATGAAAAGGGGGAGAAAGCTATGGTCTGGCAAAATTCGTGGGCGTATAGTACTAGAACGGTAGTATTTTGATACTTGCATTTGTCCGAGCTTTTAGGTGTCGTGTCGGAGTGAATATCTGCGTCTGTTAATCTTGTCTTTCTGAATCGTTTGCAGATTGGTGTGATGGTTATGGTTCATGGGGATGACAAGGGATTGGTGCTGCCTCCAAAAGTTGCGTCGGTTCAAGTTATTATCGTTCCCGTACCTTACAAAGATGCAGACACTCAAGGAATTTTTGATGCTTGTTCTGCCACTTTGGATACATTGTCCAAAGCAGGAATTCGTGCCGAGGTCGATTCTAGGGATAATTATTCTCCTGGATGGAAGTATTCTCACTGGGAAATGAAAGGTGTTCCGCTTAGGATCGAAATAGGGCCGAAGGACTTGGCAAGCAATCAGGTTGTTTATTCTGTCTACTGATGTTATTTTTCGTTACCATCGAAATAATCCTTCCCGTTGCTAACACCACATGTATTACTTTATTTCTAGGTACGTGCCGTTCGTCGTGATAACTCGGGGAAAAAGGACATACCTAGAGCTTCGTTGGTTGAACAAGTGAAAGAATTGCTCGAAAGTATTCAACAAAGCCTGTTCGACGCGGCGAAAGAAAAACGAGATGTATGCATTCAAGTTATTAATACATGGGAAGAGTTTACTGAAGCACTCAGTCAGAAGAAAATGATATTGGCTCCATGGTGTGATGAAGAGGTATTAAGATTTTGTTGCTTGTGTTGGCTGCTTCAGCTTTAACATAATATTCCATTGTTTTGGACATCAGTGATCACTGGCTTGAGTAAATTGAGAGAGGTTTTCTGATATAAGAAGTTTGTAACATGTTTGCTTTGTGCCAACTTTTTATGTCTACCGGTTTCGTTTTTATGTTCCTTTAGGAGGTCGAGAAGGACGTGAAAACAAGGACCAAGGGTGAGATGGGAGCAGCTAAAACCTTATGCTCCCCGTTCGAGCAGCCCGCACTCCCAGAAGGTACTAAATTTTTGTTCAAATCTGTATTTGTTTTTGTAACGACCCAAGCCTACTACTAGCAGATATTGTTCTTTTGAGTTGTTTTCTTTCGAGCTTCCCTTCAAGGTTTTTAAAACGCATCTACTAGGGAGAGGTTTCCACACCCTTATAAAAAATGTTTCGTTCTTCTCGCCAGCGAGTACACTGAGCTTCGAAGGGGAGTGGAGTAGATTGTGAGATCTTACATCGGTTGGAGAGGAGAGCGAAACATTCTTTATATAAGGGTGTGAAAACCTCTCCTTAGTAGACACGTTTTAAGTGAGGTTGGCGATGATACCTAATGAGCCAAAGCAGACAATATCTACAGTGGTAAGCTTGGACGGTTACAATTTTAGAAACTCTCTTTGGTTTCCTTGAATTTACTGGGATGTTTGTGGTCAGGTACCAAATGTTTTGCATCTGGGAAGCCTGCAAAGAAATGGAGCTATTGGGGTCGAAGCTACTAAGCTATCAGCTCGTTTGTGTTTGATACGGTGTGCCTGGATATAGCGTGTCAAGAAATCGAGGGGCGTTCGATTCCGTTAATTGTGAGTTCATCAGAATTCCCAATAGGGATAGTTAACTCGATGAAGAACAAAGCATTCACTTCGGCAAGTTAACTATAGAACACCTTTTATTCTGTTAGCATAGTTAGGTTTGTTCTGAGATTTTGTTATACATATTTTCACATTTACATATTTTAACAAATGTCGGAAGATTAGGCGATAGTTGAAGAGCCACAAATTCCCTATACATCTCGGGCACTTGTCAAAAAGATTAGAAAATAGGAAGAAAATTAACCAATAAA

mRNA sequence

ATGGCTGGTCCTAAGCCTGGTAGCTCTGCCACTAAACCAAAAGCTGGCGGGAAAAAGGAGGTGAAGAAAGAGACTGGGTTGGGTCTCACCAATAAGAAGGACGACAACTTCGGAGAATGGTATTCCGAGGTCGTTGTCAGTGGGGAAATGATCGAGTATTATGATATCTCGGGCTGCTATATACTGAGGCCGTGGGCGATGTCTATCTGGGAGACTATGCAAGTATTTTTTGATGCTGAAATTAAGAAAATGAAAATCAAGAATTGCTATTTTCCACTTTTTGTATCTCCCGGCGTCTTACAAAGAGAGAAAGATCATATAGAAGGTTTTGCTCCTGAGGTTGCTTGGGTAACAAAATCTGGGGAGTCTGACTTGGAAGTGCCCATTGCGATTCGTCCTACAAGTGAGACTGTTATGTACCCGTACTATTCGAAGTGGATCAGGGGTCACCGTGACTTGCCTTTGAAACTTAACCAGTGGTGCAATGTTGTGAGATGGGAATTCAGCCATCCCACACCATTTATTAGGAGTCGAGAGTTCCTTTGGCAGGAAGGTCATACTGCGTTTGCCACTAAGGATGAAGCAGATGCGGAGGTTCTTGAAATATTGGAATTGTATAGGCGGATATACGAAGAATACCTGGCCATTCCTGTTATCAAGGGAAAGAAGAGCGAGATGGAGAAGTTTGCCGGTGGTCTTTACACCACGAGCGTGGAGGCATTTATCCCGAACACTGGTCGTGGAATTCAGGGTGCGACTTCACATTGCTTGGGTCAAAATTTCGCAAAAATGTTCGAAATCAACTTTGAAAATGAAAAGGGGGAGAAAGCTATGGTCTGGCAAAATTCGTGGGCGTATAGTACTAGAACGATTGGTGTGATGGTTATGGTTCATGGGGATGACAAGGGATTGGTGCTGCCTCCAAAAGTTGCGTCGGTTCAAGTTATTATCGTTCCCGTACCTTACAAAGATGCAGACACTCAAGGAATTTTTGATGCTTGTTCTGCCACTTTGGATACATTGTCCAAAGCAGGAATTCGTGCCGAGGTCGATTCTAGGGATAATTATTCTCCTGGATGGAAGTATTCTCACTGGGAAATGAAAGGTGTTCCGCTTAGGATCGAAATAGGGCCGAAGGACTTGGCAAGCAATCAGGTACGTGCCGTTCGTCGTGATAACTCGGGGAAAAAGGACATACCTAGAGCTTCGTTGGTTGAACAAGTGAAAGAATTGCTCGAAAGTATTCAACAAAGCCTGTTCGACGCGGCGAAAGAAAAACGAGATGTATGCATTCAAGTTATTAATACATGGGAAGAGTTTACTGAAGCACTCAGTCAGAAGAAAATGATATTGGCTCCATGGTGTGATGAAGAGGAGGTCGAGAAGGACGTGAAAACAAGGACCAAGGGTGAGATGGGAGCAGCTAAAACCTTATGCTCCCCGTTCGAGCAGCCCGCACTCCCAGAAGGTACCAAATGTTTTGCATCTGGGAAGCCTGCAAAGAAATGGAGCTATTGGGGTCGAAGCTACTAAGCTATCAGCTCGTTTGTGTTTGATACGGTGTGCCTGGATATAGCGTGTCAAGAAATCGAGGGGCGTTCGATTCCGTTAATTGTGAGTTCATCAGAATTCCCAATAGGGATAGTTAACTCGATGAAGAACAAAGCATTCACTTCGGCAAGTTAACTATAGAACACCTTTTATTCTGTTAGCATAGTTAGGTTTGTTCTGAGATTTTGTTATACATATTTTCACATTTACATATTTTAACAAATGTCGGAAGATTAGGCGATAGTTGAAGAGCCACAAATTCCCTATACATCTCGGGCACTTGTCAAAAAGATTAGAAAATAGGAAGAAAATTAACCAATAAA

Coding sequence (CDS)

ATGGCTGGTCCTAAGCCTGGTAGCTCTGCCACTAAACCAAAAGCTGGCGGGAAAAAGGAGGTGAAGAAAGAGACTGGGTTGGGTCTCACCAATAAGAAGGACGACAACTTCGGAGAATGGTATTCCGAGGTCGTTGTCAGTGGGGAAATGATCGAGTATTATGATATCTCGGGCTGCTATATACTGAGGCCGTGGGCGATGTCTATCTGGGAGACTATGCAAGTATTTTTTGATGCTGAAATTAAGAAAATGAAAATCAAGAATTGCTATTTTCCACTTTTTGTATCTCCCGGCGTCTTACAAAGAGAGAAAGATCATATAGAAGGTTTTGCTCCTGAGGTTGCTTGGGTAACAAAATCTGGGGAGTCTGACTTGGAAGTGCCCATTGCGATTCGTCCTACAAGTGAGACTGTTATGTACCCGTACTATTCGAAGTGGATCAGGGGTCACCGTGACTTGCCTTTGAAACTTAACCAGTGGTGCAATGTTGTGAGATGGGAATTCAGCCATCCCACACCATTTATTAGGAGTCGAGAGTTCCTTTGGCAGGAAGGTCATACTGCGTTTGCCACTAAGGATGAAGCAGATGCGGAGGTTCTTGAAATATTGGAATTGTATAGGCGGATATACGAAGAATACCTGGCCATTCCTGTTATCAAGGGAAAGAAGAGCGAGATGGAGAAGTTTGCCGGTGGTCTTTACACCACGAGCGTGGAGGCATTTATCCCGAACACTGGTCGTGGAATTCAGGGTGCGACTTCACATTGCTTGGGTCAAAATTTCGCAAAAATGTTCGAAATCAACTTTGAAAATGAAAAGGGGGAGAAAGCTATGGTCTGGCAAAATTCGTGGGCGTATAGTACTAGAACGATTGGTGTGATGGTTATGGTTCATGGGGATGACAAGGGATTGGTGCTGCCTCCAAAAGTTGCGTCGGTTCAAGTTATTATCGTTCCCGTACCTTACAAAGATGCAGACACTCAAGGAATTTTTGATGCTTGTTCTGCCACTTTGGATACATTGTCCAAAGCAGGAATTCGTGCCGAGGTCGATTCTAGGGATAATTATTCTCCTGGATGGAAGTATTCTCACTGGGAAATGAAAGGTGTTCCGCTTAGGATCGAAATAGGGCCGAAGGACTTGGCAAGCAATCAGGTACGTGCCGTTCGTCGTGATAACTCGGGGAAAAAGGACATACCTAGAGCTTCGTTGGTTGAACAAGTGAAAGAATTGCTCGAAAGTATTCAACAAAGCCTGTTCGACGCGGCGAAAGAAAAACGAGATGTATGCATTCAAGTTATTAATACATGGGAAGAGTTTACTGAAGCACTCAGTCAGAAGAAAATGATATTGGCTCCATGGTGTGATGAAGAGGAGGTCGAGAAGGACGTGAAAACAAGGACCAAGGGTGAGATGGGAGCAGCTAAAACCTTATGCTCCCCGTTCGAGCAGCCCGCACTCCCAGAAGGTACCAAATGTTTTGCATCTGGGAAGCCTGCAAAGAAATGGAGCTATTGGGGTCGAAGCTACTAA

Protein sequence

MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY
Homology
BLAST of CmaCh14G014790 vs. ExPASy Swiss-Prot
Match: Q9M1R2 (Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 PE=1 SV=1)

HSP 1 Score: 886.3 bits (2289), Expect = 1.6e-256
Identity = 412/493 (83.57%), Postives = 456/493 (92.49%), Query Frame = 0

Query: 18  KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETMQVFF 77
           KK+VKKETGLGL+ KKD+NFGEWYSE V   +MIEYYDISGCYILRPW+M+IWE MQ+FF
Sbjct: 39  KKDVKKETGLGLSVKKDENFGEWYSE-VCKQDMIEYYDISGCYILRPWSMAIWEIMQIFF 98

Query: 78  DAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRPTSET 137
           DAEIKKMK+KNCYFPLFVSPGVL++EKDHIEGFAPEVAWVTKSG+SDLEVPIAIRPTSET
Sbjct: 99  DAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEVPIAIRPTSET 158

Query: 138 VMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQEGHTAFATKDEADA 197
           VMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSREFLWQEGHTAFATK EAD 
Sbjct: 159 VMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATKAEADE 218

Query: 198 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 257
           EVL+ILELYRRIYEEYLA+PV+KG KSE EKFAGGLYTTSVEAFIPNTGRG+QGATSHCL
Sbjct: 219 EVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGRGVQGATSHCL 278

Query: 258 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVII 317
           GQNFAKMFEINFENEK E  MVWQNSWAYSTRTIGVM+M HGDDKGLVLPPKVASVQV++
Sbjct: 279 GQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLPPKVASVQVVV 338

Query: 318 VPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIG 377
           +PVPYKDA+TQGI+DAC+AT   L +AGIRAE D RDNYSPGWKYS WEMKGVPLRIEIG
Sbjct: 339 IPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLRIEIG 398

Query: 378 PKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSLFDAAKEKRDVCIQVINTW 437
           P+DL ++QVR VRRDN  K+DIPR SLVE VKELLE IQQ++++ AK+KR+ C+Q + TW
Sbjct: 399 PRDLENDQVRTVRRDNGVKEDIPRGSLVEHVKELLEKIQQNMYEVAKQKREACVQEVKTW 458

Query: 438 EEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASG 497
           +EF +AL++KK+ILAPWCDEEEVE+DVK RTKGE GAAKTLCSPF+QP LPEGT CFASG
Sbjct: 459 DEFIKALNEKKLILAPWCDEEEVERDVKARTKGETGAAKTLCSPFDQPELPEGTLCFASG 518

Query: 498 KPAKKWSYWGRSY 511
           KPAKKW+YWGRSY
Sbjct: 519 KPAKKWTYWGRSY 530

BLAST of CmaCh14G014790 vs. ExPASy Swiss-Prot
Match: P28668 (Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster OX=7227 GN=GluProRS PE=1 SV=2)

HSP 1 Score: 579.3 bits (1492), Expect = 4.2e-164
Identity = 293/527 (55.60%), Postives = 375/527 (71.16%), Query Frame = 0

Query: 2    AGPKPGSSATK-PKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 61
            A PKP     K P A     VKK+T LGL   K+DN  +WYS+V+  GEMIEYYD+SGCY
Sbjct: 1189 AQPKPAKPVKKEPAADASGAVKKQTRLGLEATKEDNLPDWYSQVITKGEMIEYYDVSGCY 1248

Query: 62   ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 121
            ILR W+ +IW+ ++ +FDAEI +M +K CYFP+FVS  VL++EK HI  FAPEVAWVTKS
Sbjct: 1249 ILRQWSFAIWKAIKTWFDAEITRMGVKECYFPIFVSKAVLEKEKTHIADFAPEVAWVTKS 1308

Query: 122  GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 181
            G+SDL  PIA+RPTSETVMYP Y+KW++ +RDLP++LNQW NVVRWEF  PTPF+R+REF
Sbjct: 1309 GDSDLAEPIAVRPTSETVMYPAYAKWVQSYRDLPIRLNQWNNVVRWEFKQPTPFLRTREF 1368

Query: 182  LWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 241
            LWQEGHTAFA K+EA  EVL+IL+LY  +Y   LAIPV+KG+K+E EKFAGG YTT+VEA
Sbjct: 1369 LWQEGHTAFADKEEAAKEVLDILDLYALVYTHLLAIPVVKGRKTEKEKFAGGDYTTTVEA 1428

Query: 242  FIPNTGRGIQGATSHCLGQNFAKMFEINFEN-EKGEKAMVWQNSWAYSTRTIGVMVMVHG 301
            FI  +GR IQGATSH LGQNF+KMFEI +E+ E  +K  V+QNSW  +TRTIGVM+MVH 
Sbjct: 1429 FISASGRAIQGATSHHLGQNFSKMFEIVYEDPETQQKKYVYQNSWGITTRTIGVMIMVHA 1488

Query: 302  DDKGLVLPPKVASVQVIIVP----VPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDN 361
            D++GLVLPP VA +Q I+VP    V  KD +   + DAC A    L   G+R E D RDN
Sbjct: 1489 DNQGLVLPPHVACIQAIVVPCGITVNTKDDERAQLLDACKALEKRLVGGGVRCEGDYRDN 1548

Query: 362  YSPGWKYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESI 421
            YSPGWK++HWE+KGVPLR+E+GPKDL + Q+ AVRRD   K  IP A + +++  LLE+I
Sbjct: 1549 YSPGWKFNHWELKGVPLRLEVGPKDLKAQQLVAVRRDTVEKITIPLADVEKKIPALLETI 1608

Query: 422  QQSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRT----KGE 481
             +S+ + A+E      + +  W +F   L QK ++LAP+C E   E  +K  +    + E
Sbjct: 1609 HESMLNKAQEDMTSHTKKVTNWTDFCGFLEQKNILLAPFCGEISCEDKIKADSARGEEAE 1668

Query: 482  MGA----AKTLCSPFEQPA-LPEGTKCF---ASGKPAKKWSYWGRSY 511
             GA    AK+LC PF+QPA +    KC     + KP K ++ +GRSY
Sbjct: 1669 PGAPAMGAKSLCIPFDQPAPIAASDKCINPSCTNKP-KFYTLFGRSY 1714

BLAST of CmaCh14G014790 vs. ExPASy Swiss-Prot
Match: Q8CGC7 (Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs1 PE=1 SV=4)

HSP 1 Score: 579.3 bits (1492), Expect = 4.2e-164
Identity = 291/519 (56.07%), Postives = 370/519 (71.29%), Query Frame = 0

Query: 7    GSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWA 66
            GS  +   AG  +  KK+T LGL  KK++N  EWYS+V+   EMIEYYD+SGCYILRPW+
Sbjct: 994  GSGLSSGGAGEGQGPKKQTRLGLEAKKEENLAEWYSQVITKSEMIEYYDVSGCYILRPWS 1053

Query: 67   MSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLE 126
             SIWE+++ FFDAEIKK+ ++NCYFP+FVS   L++EK+HIE FAPEVAWVT+SG+++L 
Sbjct: 1054 YSIWESIKDFFDAEIKKLGVENCYFPIFVSQAALEKEKNHIEDFAPEVAWVTRSGKTELA 1113

Query: 127  VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQEGH 186
             PIAIRPTSETVMYP Y+KW++ HRDLP++LNQWCNVVRWEF HP PF+R+REFLWQEGH
Sbjct: 1114 EPIAIRPTSETVMYPAYAKWVQSHRDLPVRLNQWCNVVRWEFKHPQPFLRTREFLWQEGH 1173

Query: 187  TAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTG 246
            +AFAT +EA  EVL+ILELY R+YEE LAIPV++G+K+E EKFAGG YTT++EAFI  +G
Sbjct: 1174 SAFATFEEAADEVLQILELYARVYEELLAIPVVRGRKTEKEKFAGGDYTTTIEAFISASG 1233

Query: 247  RGIQGATSHCLGQNFAKMFEINFENEK--GEKAMVWQNSWAYSTRTIGVMVMVHGDDKGL 306
            R IQGATSH LGQNF+KM EI FE+ K  GEK   +Q SW  +TRTIGVMVMVHGD+ GL
Sbjct: 1234 RAIQGATSHHLGQNFSKMCEIVFEDPKTPGEKQFAYQCSWGLTTRTIGVMVMVHGDNMGL 1293

Query: 307  VLPPKVASVQVIIVPVPYKDA----DTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPGW 366
            VLPP+VASVQV+++P    +A    D + +   C+     L  A IR  VD RDNYSPGW
Sbjct: 1294 VLPPRVASVQVVVIPCGITNALSEEDREALMAKCNEYRRRLLGANIRVRVDLRDNYSPGW 1353

Query: 367  KYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSLF 426
            K++HWE+KGVP+R+E+GP+D+ S Q  AVRRD   K  I       +++++LE IQ +LF
Sbjct: 1354 KFNHWELKGVPVRLEVGPRDMKSCQFVAVRRDTGEKLTIAEKEAEAKLEKVLEDIQLNLF 1413

Query: 427  DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKG----EMGA-- 486
              A E     + V NT E+F + L   K+   P+C E + E  +K  T      E GA  
Sbjct: 1414 TRASEDLKTHMVVSNTLEDFQKVLDAGKVAQIPFCGEIDCEDWIKKMTARDQDVEPGAPS 1473

Query: 487  --AKTLCSPFEQPA-LPEGTKCFASGKPAKKWSYWGRSY 511
              AK+LC PF     L  G  C     PAK ++ +GRSY
Sbjct: 1474 MGAKSLCIPFNPLCELQPGAMCVCGKNPAKFYTLFGRSY 1512

BLAST of CmaCh14G014790 vs. ExPASy Swiss-Prot
Match: O60155 (Putative proline--tRNA ligase C19C7.06 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=prs1 PE=3 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 1.6e-163
Identity = 294/546 (53.85%), Postives = 370/546 (67.77%), Query Frame = 0

Query: 2   AGPKPGSSATKPKAGGKK--------EVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEY 61
           + P   ++ +KP A  KK         ++    +G+T +KD +F  WY +V+   +MIEY
Sbjct: 172 SAPGSATAPSKPAAQKKKAEPSKNDAAIENAALIGITVRKDADFPNWYQQVLTKSDMIEY 231

Query: 62  YDISGCYILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE 121
           YDISGCYIL+PW+ SIWE +Q +FD EIKK+ ++N YFPLFVS  VL++EKDH+EGFAPE
Sbjct: 232 YDISGCYILKPWSYSIWEAIQGWFDKEIKKLGVRNGYFPLFVSSKVLEKEKDHVEGFAPE 291

Query: 122 VAWVTKSGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTP 181
           VAWVT++G S+L+ PIAIRPTSETVMYPYY+KWIR HRDLPLKLNQW +VVRWEF +P P
Sbjct: 292 VAWVTRAGTSELDEPIAIRPTSETVMYPYYAKWIRSHRDLPLKLNQWNSVVRWEFKNPQP 351

Query: 182 FIRSREFLWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGL 241
           F+R+REFLWQEGHTA  T + A  EV +IL+LY RIY + LA+PVIKG KSE EKFAGG+
Sbjct: 352 FLRTREFLWQEGHTAHMTLEGATEEVHQILDLYARIYTDLLAVPVIKGVKSENEKFAGGM 411

Query: 242 YTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGE--------KAMVWQNSWA 301
           +TT+VE +IP TGRGIQGATSHCLGQNF+KMF I  E+   E        K  VWQNSW 
Sbjct: 412 FTTTVEGYIPTTGRGIQGATSHCLGQNFSKMFNIVVEDPNAEIGPTGERPKLFVWQNSWG 471

Query: 302 YSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPV----PYKDADTQGIFDACSATLDTL 361
            STRTIGV VMVHGDDKGL LPP +A VQ ++VP        D +   I   CS   D L
Sbjct: 472 LSTRTIGVAVMVHGDDKGLKLPPAIALVQSVVVPCGITNKTTDQERNEIEGFCSKLADRL 531

Query: 362 SKAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPR 421
           + A IR E D R  Y+PG+K+SHWEMKGVPLR+E GP D   NQV AVRRD   K  +P 
Sbjct: 532 NAADIRTEADLR-AYTPGYKFSHWEMKGVPLRLEYGPNDAKKNQVTAVRRDTFEKIPVPL 591

Query: 422 ASLVEQVKELLESIQQSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVE 481
            +L + V +LL  IQ ++++ AK +RD  +  +  W +F  AL++K +++ PWC+  E E
Sbjct: 592 NNLEKGVSDLLAKIQTNMYETAKAERDAHVVKVKEWADFVPALNKKNIVMIPWCNTTECE 651

Query: 482 KDVKTRT------------KGEMGAAKTLCSPFEQP----ALPEG-TKCFASGKPAKKWS 511
           K++K  +            K     AK+LC P EQP    A+ EG TKC   G  AK W 
Sbjct: 652 KEIKKNSARQVNGDEPEDEKAPSMGAKSLCIPLEQPSGEDAIIEGTTKCAGCGNLAKVWG 711

BLAST of CmaCh14G014790 vs. ExPASy Swiss-Prot
Match: P07814 (Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens OX=9606 GN=EPRS1 PE=1 SV=5)

HSP 1 Score: 576.6 bits (1485), Expect = 2.7e-163
Identity = 285/519 (54.91%), Postives = 365/519 (70.33%), Query Frame = 0

Query: 7    GSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWA 66
            G   +   AG  +  KK+T LGL  KK++N  +WYS+V+   EMIEY+DISGCYILRPWA
Sbjct: 994  GGGLSSSGAGEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWA 1053

Query: 67   MSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLE 126
             +IWE ++ FFDAEIKK+ ++NCYFP+FVS   L++EK H+  FAPEVAWVT+SG+++L 
Sbjct: 1054 YAIWEAIKDFFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELA 1113

Query: 127  VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQEGH 186
             PIAIRPTSETVMYP Y+KW++ HRDLP+KLNQWCNVVRWEF HP PF+R+REFLWQEGH
Sbjct: 1114 EPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGH 1173

Query: 187  TAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTG 246
            +AFAT +EA  EVL+IL+LY ++YEE LAIPV+KG+K+E EKFAGG YTT++EAFI  +G
Sbjct: 1174 SAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASG 1233

Query: 247  RGIQGATSHCLGQNFAKMFEINFENEK--GEKAMVWQNSWAYSTRTIGVMVMVHGDDKGL 306
            R IQG TSH LGQNF+KMFEI FE+ K  GEK   +QNSW  +TRTIGVM MVHGD+ GL
Sbjct: 1234 RAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGL 1293

Query: 307  VLPPKVASVQVIIVPVPYKDA----DTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPGW 366
            VLPP+VA VQV+I+P    +A    D + +   C+     L    IR   D RDNYSPGW
Sbjct: 1294 VLPPRVACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGW 1353

Query: 367  KYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSLF 426
            K++HWE+KGVP+R+E+GP+D+ S Q  AVRRD   K  +       +++ +LE IQ +LF
Sbjct: 1354 KFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLF 1413

Query: 427  DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKG----EMGA-- 486
              A E     + V NT E+F + L   K++  P+C E + E  +K  T      E GA  
Sbjct: 1414 TRASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPS 1473

Query: 487  --AKTLCSPFEQPA-LPEGTKCFASGKPAKKWSYWGRSY 511
              AK+LC PF+    L  G KC     PAK ++ +GRSY
Sbjct: 1474 MGAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY 1512

BLAST of CmaCh14G014790 vs. ExPASy TrEMBL
Match: A0A6J1IRP0 (Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111479369 PE=3 SV=1)

HSP 1 Score: 1051.2 bits (2717), Expect = 1.4e-303
Identity = 510/510 (100.00%), Postives = 510/510 (100.00%), Query Frame = 0

Query: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60
           MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY
Sbjct: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60

Query: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120
           ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS
Sbjct: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120

Query: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180
           GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF
Sbjct: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180

Query: 181 LWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240
           LWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA
Sbjct: 181 LWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240

Query: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300
           FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD
Sbjct: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300

Query: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPGW 360
           DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPGW
Sbjct: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPGW 360

Query: 361 KYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSLF 420
           KYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSLF
Sbjct: 361 KYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSLF 420

Query: 421 DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480
           DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS
Sbjct: 421 DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480

Query: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 511
           PFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

BLAST of CmaCh14G014790 vs. ExPASy TrEMBL
Match: A0A6J1GUJ5 (Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111457586 PE=3 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 1.7e-301
Identity = 505/510 (99.02%), Postives = 508/510 (99.61%), Query Frame = 0

Query: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60
           MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY
Sbjct: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60

Query: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120
           ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS
Sbjct: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120

Query: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180
           GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF
Sbjct: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180

Query: 181 LWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240
           LWQEGHTAFATKDEAD EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA
Sbjct: 181 LWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240

Query: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300
           FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD
Sbjct: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300

Query: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPGW 360
           DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACS TLDTLS+AGIRAEVDSRDNYSPGW
Sbjct: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGW 360

Query: 361 KYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSLF 420
           KYSHWEMKGVPLRIEIGPKDLA+NQVRAVRRDNSGKKDIPRA+LVEQVKELLESIQQSLF
Sbjct: 361 KYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLF 420

Query: 421 DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480
           DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS
Sbjct: 421 DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480

Query: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 511
           PFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

BLAST of CmaCh14G014790 vs. ExPASy TrEMBL
Match: A0A6J1GYJ1 (Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111458443 PE=3 SV=1)

HSP 1 Score: 1025.0 bits (2649), Expect = 1.1e-295
Identity = 497/511 (97.26%), Postives = 502/511 (98.24%), Query Frame = 0

Query: 1   MAGPKPGSSATKPKAGG-KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSA  PKAGG KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSAKTPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEAD EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGIFDACS TLDTLS+AGIRAEVD+RDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSVTLDTLSEAGIRAEVDARDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLA+NQVRAVRRDNS KKDIPRASLVEQVKELL+SIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRASLVEQVKELLDSIQQSL 420

Query: 421 FDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAK KRD CIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 511
           SPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFEQPELPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of CmaCh14G014790 vs. ExPASy TrEMBL
Match: A0A6J1JLY7 (Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111487085 PE=3 SV=1)

HSP 1 Score: 1023.5 bits (2645), Expect = 3.1e-295
Identity = 496/511 (97.06%), Postives = 501/511 (98.04%), Query Frame = 0

Query: 1   MAGPKPGSSATKPKAGG-KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSA  PKAGG KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSAKTPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEAD EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGIFDACS TLDTLS+AGIRAEVD+RDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSVTLDTLSEAGIRAEVDARDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLA+NQVRAVRRDNS KKDIPRASLVEQVKELL+SIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRASLVEQVKELLDSIQQSL 420

Query: 421 FDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAK KRD CIQVINTWEEFTEAL QKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 511
           SPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFEQPELPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of CmaCh14G014790 vs. ExPASy TrEMBL
Match: A0A0A0KTZ1 (Prolyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_4G015750 PE=3 SV=1)

HSP 1 Score: 1023.1 bits (2644), Expect = 4.1e-295
Identity = 497/511 (97.26%), Postives = 502/511 (98.24%), Query Frame = 0

Query: 1   MAGPKPGSSATKPKAGG-KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSAT  KAGG KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATNKKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWA+SIWETMQVFFDAEIK+MKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAISIWETMQVFFDAEIKQMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEAD EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTL+ AGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLTAAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLA+NQVRAVRRDNSGKKDIPR SLVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRDSLVEQVKELLESIQQSL 420

Query: 421 FDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAK KRD CIQVINTWEEFTEAL QKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 511
           SPF+QPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFDQPALPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of CmaCh14G014790 vs. NCBI nr
Match: XP_022979761.1 (proline--tRNA ligase, cytoplasmic-like [Cucurbita maxima])

HSP 1 Score: 1051.2 bits (2717), Expect = 2.9e-303
Identity = 510/510 (100.00%), Postives = 510/510 (100.00%), Query Frame = 0

Query: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60
           MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY
Sbjct: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60

Query: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120
           ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS
Sbjct: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120

Query: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180
           GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF
Sbjct: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180

Query: 181 LWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240
           LWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA
Sbjct: 181 LWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240

Query: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300
           FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD
Sbjct: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300

Query: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPGW 360
           DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPGW
Sbjct: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPGW 360

Query: 361 KYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSLF 420
           KYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSLF
Sbjct: 361 KYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSLF 420

Query: 421 DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480
           DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS
Sbjct: 421 DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480

Query: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 511
           PFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

BLAST of CmaCh14G014790 vs. NCBI nr
Match: XP_022955643.1 (proline--tRNA ligase, cytoplasmic isoform X1 [Cucurbita moschata] >KAG6582053.1 Proline--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1044.3 bits (2699), Expect = 3.5e-301
Identity = 505/510 (99.02%), Postives = 508/510 (99.61%), Query Frame = 0

Query: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60
           MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY
Sbjct: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60

Query: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120
           ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS
Sbjct: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120

Query: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180
           GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF
Sbjct: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180

Query: 181 LWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240
           LWQEGHTAFATKDEAD EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA
Sbjct: 181 LWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240

Query: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300
           FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD
Sbjct: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300

Query: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPGW 360
           DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACS TLDTLS+AGIRAEVDSRDNYSPGW
Sbjct: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGW 360

Query: 361 KYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSLF 420
           KYSHWEMKGVPLRIEIGPKDLA+NQVRAVRRDNSGKKDIPRA+LVEQVKELLESIQQSLF
Sbjct: 361 KYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLF 420

Query: 421 DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480
           DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS
Sbjct: 421 DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480

Query: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 511
           PFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

BLAST of CmaCh14G014790 vs. NCBI nr
Match: XP_023528664.1 (proline--tRNA ligase, cytoplasmic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1042.0 bits (2693), Expect = 1.7e-300
Identity = 504/510 (98.82%), Postives = 507/510 (99.41%), Query Frame = 0

Query: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60
           MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY
Sbjct: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60

Query: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120
           ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS
Sbjct: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120

Query: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180
           GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF
Sbjct: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180

Query: 181 LWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240
           LWQEGHTAFATKDEAD EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA
Sbjct: 181 LWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240

Query: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300
           FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD
Sbjct: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300

Query: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPGW 360
           DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACS TLDTLS+AGIRAEVDSRDNYSPGW
Sbjct: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGW 360

Query: 361 KYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSLF 420
           KYSHWEMKGVPLRIEIGPKDLA+NQVRAVRRDNSGKKDIPRA+LVEQVKELLESIQQSLF
Sbjct: 361 KYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLF 420

Query: 421 DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480
           DAAKEKRDVCIQVI TWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS
Sbjct: 421 DAAKEKRDVCIQVITTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480

Query: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 511
           PFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

BLAST of CmaCh14G014790 vs. NCBI nr
Match: XP_038874652.1 (proline--tRNA ligase, cytoplasmic isoform X1 [Benincasa hispida])

HSP 1 Score: 1030.8 bits (2664), Expect = 4.0e-297
Identity = 499/511 (97.65%), Postives = 505/511 (98.83%), Query Frame = 0

Query: 1   MAGPKPGSSATKPKAGG-KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSS T PKAGG KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSTTNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWA+S+WETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAISVWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEAD EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLA+NQVRAVRRDNSGKKDIPRASLVEQVKELL+SIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRASLVEQVKELLDSIQQSL 420

Query: 421 FDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAKEKRD CI+VINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKEKRDACIKVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 511
           SPF+QP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFQQPDLPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of CmaCh14G014790 vs. NCBI nr
Match: XP_038907011.1 (proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907020.1 proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907029.1 proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1029.6 bits (2661), Expect = 8.9e-297
Identity = 501/511 (98.04%), Postives = 504/511 (98.63%), Query Frame = 0

Query: 1   MAGPKPGSSATKPKAGG-KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSAT PKAGG KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWA+SIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAISIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEAD EVLEILELYR IYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRCIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLS+AGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLA+NQVRAVRRDNSGKKDIPR SLVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRDSLVEQVKELLESIQQSL 420

Query: 421 FDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAKEKRD CIQVINTWEEFTEAL QKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKEKRDKCIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 511
           SPFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 511

BLAST of CmaCh14G014790 vs. TAIR 10
Match: AT3G62120.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 886.3 bits (2289), Expect = 1.1e-257
Identity = 412/493 (83.57%), Postives = 456/493 (92.49%), Query Frame = 0

Query: 18  KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETMQVFF 77
           KK+VKKETGLGL+ KKD+NFGEWYSE V   +MIEYYDISGCYILRPW+M+IWE MQ+FF
Sbjct: 39  KKDVKKETGLGLSVKKDENFGEWYSE-VCKQDMIEYYDISGCYILRPWSMAIWEIMQIFF 98

Query: 78  DAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRPTSET 137
           DAEIKKMK+KNCYFPLFVSPGVL++EKDHIEGFAPEVAWVTKSG+SDLEVPIAIRPTSET
Sbjct: 99  DAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEVPIAIRPTSET 158

Query: 138 VMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQEGHTAFATKDEADA 197
           VMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSREFLWQEGHTAFATK EAD 
Sbjct: 159 VMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATKAEADE 218

Query: 198 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 257
           EVL+ILELYRRIYEEYLA+PV+KG KSE EKFAGGLYTTSVEAFIPNTGRG+QGATSHCL
Sbjct: 219 EVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGRGVQGATSHCL 278

Query: 258 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVII 317
           GQNFAKMFEINFENEK E  MVWQNSWAYSTRTIGVM+M HGDDKGLVLPPKVASVQV++
Sbjct: 279 GQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLPPKVASVQVVV 338

Query: 318 VPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIG 377
           +PVPYKDA+TQGI+DAC+AT   L +AGIRAE D RDNYSPGWKYS WEMKGVPLRIEIG
Sbjct: 339 IPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLRIEIG 398

Query: 378 PKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSLFDAAKEKRDVCIQVINTW 437
           P+DL ++QVR VRRDN  K+DIPR SLVE VKELLE IQQ++++ AK+KR+ C+Q + TW
Sbjct: 399 PRDLENDQVRTVRRDNGVKEDIPRGSLVEHVKELLEKIQQNMYEVAKQKREACVQEVKTW 458

Query: 438 EEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASG 497
           +EF +AL++KK+ILAPWCDEEEVE+DVK RTKGE GAAKTLCSPF+QP LPEGT CFASG
Sbjct: 459 DEFIKALNEKKLILAPWCDEEEVERDVKARTKGETGAAKTLCSPFDQPELPEGTLCFASG 518

Query: 498 KPAKKWSYWGRSY 511
           KPAKKW+YWGRSY
Sbjct: 519 KPAKKWTYWGRSY 530

BLAST of CmaCh14G014790 vs. TAIR 10
Match: AT3G62120.2 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 886.3 bits (2289), Expect = 1.1e-257
Identity = 412/493 (83.57%), Postives = 456/493 (92.49%), Query Frame = 0

Query: 18  KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETMQVFF 77
           KK+VKKETGLGL+ KKD+NFGEWYSE V   +MIEYYDISGCYILRPW+M+IWE MQ+FF
Sbjct: 39  KKDVKKETGLGLSVKKDENFGEWYSE-VCKQDMIEYYDISGCYILRPWSMAIWEIMQIFF 98

Query: 78  DAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRPTSET 137
           DAEIKKMK+KNCYFPLFVSPGVL++EKDHIEGFAPEVAWVTKSG+SDLEVPIAIRPTSET
Sbjct: 99  DAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEVPIAIRPTSET 158

Query: 138 VMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQEGHTAFATKDEADA 197
           VMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSREFLWQEGHTAFATK EAD 
Sbjct: 159 VMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATKAEADE 218

Query: 198 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 257
           EVL+ILELYRRIYEEYLA+PV+KG KSE EKFAGGLYTTSVEAFIPNTGRG+QGATSHCL
Sbjct: 219 EVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGRGVQGATSHCL 278

Query: 258 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVII 317
           GQNFAKMFEINFENEK E  MVWQNSWAYSTRTIGVM+M HGDDKGLVLPPKVASVQV++
Sbjct: 279 GQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLPPKVASVQVVV 338

Query: 318 VPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIG 377
           +PVPYKDA+TQGI+DAC+AT   L +AGIRAE D RDNYSPGWKYS WEMKGVPLRIEIG
Sbjct: 339 IPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLRIEIG 398

Query: 378 PKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSLFDAAKEKRDVCIQVINTW 437
           P+DL ++QVR VRRDN  K+DIPR SLVE VKELLE IQQ++++ AK+KR+ C+Q + TW
Sbjct: 399 PRDLENDQVRTVRRDNGVKEDIPRGSLVEHVKELLEKIQQNMYEVAKQKREACVQEVKTW 458

Query: 438 EEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASG 497
           +EF +AL++KK+ILAPWCDEEEVE+DVK RTKGE GAAKTLCSPF+QP LPEGT CFASG
Sbjct: 459 DEFIKALNEKKLILAPWCDEEEVERDVKARTKGETGAAKTLCSPFDQPELPEGTLCFASG 518

Query: 498 KPAKKWSYWGRSY 511
           KPAKKW+YWGRSY
Sbjct: 519 KPAKKWTYWGRSY 530

BLAST of CmaCh14G014790 vs. TAIR 10
Match: AT5G52520.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 419.1 bits (1076), Expect = 5.2e-117
Identity = 215/517 (41.59%), Postives = 312/517 (60.35%), Query Frame = 0

Query: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60
           ++G     S T        EV +         +  +F  WY +V+ S E+ +Y  + G  
Sbjct: 37  LSGFATAPSGTASPETKSSEVDRLRSDRAVTPRSQDFNAWYLDVIASAELADYGPVRGTM 96

Query: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120
           ++RP+  +IWE +Q + + + K+    N YFP F+    +++E  H+EGF+PE+A VT  
Sbjct: 97  VIRPYGYAIWEAIQDYLNVKFKETGHSNMYFPQFIPYSFIEKEASHVEGFSPELALVTVG 156

Query: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180
           G  +LE  + +RPTSET++   +++WI  +RDLPL +NQW NV RWE     PFIR+ EF
Sbjct: 157 GGKELEEKLVVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEM-RTKPFIRTLEF 216

Query: 181 LWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240
           LWQEGHTA AT +EA+ E  +++E+Y R   E  AIPVI G+KS++E FAG   T ++EA
Sbjct: 217 LWQEGHTAHATPEEAEKEAKQMIEIYTRFAFEQTAIPVIPGRKSKLETFAGADITYTIEA 276

Query: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300
            + +  + +Q  TSH LGQNF++ F   F +E GE+  VWQ SWA STR +G ++M HGD
Sbjct: 277 MMGDR-KALQAGTSHNLGQNFSRAFGTQFADENGERQHVWQTSWAVSTRFVGGIIMTHGD 336

Query: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPGW 360
           D GL+LPPK+A +QV+IVP+  KD +  G+  A S+  + L  AG+R ++D  D  +PGW
Sbjct: 337 DTGLMLPPKIAPIQVVIVPIWKKDTEKTGVLSAASSVKEALQTAGVRVKLDDTDQRTPGW 396

Query: 361 KYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKK------DIPRASLVEQVKELLES 420
           K++ WEMKG+PLRIEIGP+D++SN V   RRD  GK        +  ++LV  VKE L+ 
Sbjct: 397 KFNFWEMKGIPLRIEIGPRDVSSNSVVVSRRDVPGKAGKVFGISMEPSTLVAYVKEKLDE 456

Query: 421 IQQSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGA 480
           IQ SL + A   RD  I  +N++ E  +A+S  K    PW   +  E+ VK  T      
Sbjct: 457 IQTSLLEKALSFRDSNIVDVNSYAELKDAISSGKWARGPWSASDADEQRVKEET-----G 516

Query: 481 AKTLCSPFEQPALPEGTK-CFASGKPAKKWSYWGRSY 511
           A   C PFEQ    +GTK C  +G PA++ + + +SY
Sbjct: 517 ATIRCFPFEQ---TQGTKTCLMTGNPAEEVAIFAKSY 543

BLAST of CmaCh14G014790 vs. TAIR 10
Match: AT5G10880.1 (tRNA synthetase-related / tRNA ligase-related )

HSP 1 Score: 289.7 bits (740), Expect = 4.7e-78
Identity = 144/221 (65.16%), Postives = 166/221 (75.11%), Query Frame = 0

Query: 296 MVHGDDKGLVLPPKVASVQVIIVPVPYKD-ADTQGIFDACSATLDTLSKAGIRAEVDSRD 355
           M HGDDKGLV PPKVA VQV+++ VP K  AD Q + DAC A   TL  AGIRAE D RD
Sbjct: 89  MTHGDDKGLVFPPKVAPVQVVVIHVPIKGAADYQELCDACEAVESTLLGAGIRAEADIRD 148

Query: 356 NYSPGWKYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLES 415
           NYS GWKY+  E+ GVPLRIE GP+DLA++QVR V RDN  K D+ R  L+EQVK+LLE 
Sbjct: 149 NYSCGWKYADQELTGVPLRIETGPRDLANDQVRIVTRDNGAKMDVKRGDLIEQVKDLLEK 208

Query: 416 IQQSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEM-- 475
           IQ +L+D AK K + C Q + TW+EF EALSQKK+ILAPWCD+ EVEKDVK RT+G+   
Sbjct: 209 IQSNLYDVAKRKVEECTQKVETWDEFVEALSQKKLILAPWCDKVEVEKDVKRRTRGDETG 268

Query: 476 ---GAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 511
              G AKTLC+P EQP L E T CFASGKPAKKWSYWGRSY
Sbjct: 269 GGGGGAKTLCTPLEQPELGEETLCFASGKPAKKWSYWGRSY 309


HSP 2 Score: 62.0 bits (149), Expect = 1.6e-09
Identity = 27/47 (57.45%), Postives = 37/47 (78.72%), Query Frame = 0

Query: 23 KETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYD-ISGCYILRPWAMS 69
          KET LG+T KKD++FG+WYSE    GE++EYY+ + GCYIL+P  +S
Sbjct: 3  KETRLGITAKKDEDFGKWYSEACRFGELVEYYESVKGCYILKPSGIS 49

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M1R21.6e-25683.57Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 P... [more]
P286684.2e-16455.60Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster OX=7227 G... [more]
Q8CGC74.2e-16456.07Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs1 PE... [more]
O601551.6e-16353.85Putative proline--tRNA ligase C19C7.06 OS=Schizosaccharomyces pombe (strain 972 ... [more]
P078142.7e-16354.91Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens OX=9606 GN=EPRS1 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1IRP01.4e-303100.00Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111479369 PE=3 SV=1[more]
A0A6J1GUJ51.7e-30199.02Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111457586 PE=3 SV=1[more]
A0A6J1GYJ11.1e-29597.26Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111458443 PE=3 SV=1[more]
A0A6J1JLY73.1e-29597.06Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111487085 PE=3 SV=1[more]
A0A0A0KTZ14.1e-29597.26Prolyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_4G015750 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022979761.12.9e-303100.00proline--tRNA ligase, cytoplasmic-like [Cucurbita maxima][more]
XP_022955643.13.5e-30199.02proline--tRNA ligase, cytoplasmic isoform X1 [Cucurbita moschata] >KAG6582053.1 ... [more]
XP_023528664.11.7e-30098.82proline--tRNA ligase, cytoplasmic isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_038874652.14.0e-29797.65proline--tRNA ligase, cytoplasmic isoform X1 [Benincasa hispida][more]
XP_038907011.18.9e-29798.04proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907... [more]
Match NameE-valueIdentityDescription
AT3G62120.11.1e-25783.57Class II aaRS and biotin synthetases superfamily protein [more]
AT3G62120.21.1e-25783.57Class II aaRS and biotin synthetases superfamily protein [more]
AT5G52520.15.2e-11741.59Class II aaRS and biotin synthetases superfamily protein [more]
AT5G10880.14.7e-7865.16tRNA synthetase-related / tRNA ligase-related [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002316Proline-tRNA ligase, class IIaPRINTSPR01046TRNASYNTHPROcoord: 129..140
score: 49.38
coord: 159..167
score: 48.97
coord: 169..180
score: 42.59
coord: 88..106
score: 25.15
IPR016061Proline-tRNA ligase, class II, C-terminalSMARTSM00946ProRS_C_1_2coord: 437..510
e-value: 2.2E-28
score: 110.3
IPR016061Proline-tRNA ligase, class II, C-terminalPFAMPF09180ProRS-C_1coord: 437..510
e-value: 3.2E-26
score: 91.4
IPR004499Proline-tRNA ligase, class IIa, archaeal-typeTIGRFAMTIGR00408TIGR00408coord: 31..510
e-value: 7.4E-180
score: 596.8
IPR004499Proline-tRNA ligase, class IIa, archaeal-typePANTHERPTHR43382PROLYL-TRNA SYNTHETASEcoord: 10..510
IPR004499Proline-tRNA ligase, class IIa, archaeal-typeHAMAPMF_01571Pro_tRNA_synth_type3coord: 30..510
score: 38.222679
IPR017449Prolyl-tRNA synthetase, class IIGENE3D3.30.110.30coord: 431..510
e-value: 2.0E-28
score: 100.5
IPR017449Prolyl-tRNA synthetase, class IISUPERFAMILY64586C-terminal domain of ProRScoord: 432..510
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 30..307
e-value: 2.0E-110
score: 370.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availablePANTHERPTHR43382:SF4PROLINE--TRNA LIGASE, CYTOPLASMIC-LIKEcoord: 10..510
NoneNo IPR availableCDDcd00862ProRS_anticodon_zinccoord: 303..510
e-value: 3.3146E-93
score: 280.338
NoneNo IPR availableSUPERFAMILY52954Class II aaRS ABD-relatedcoord: 302..427
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 35..301
IPR002314Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)PFAMPF00587tRNA-synt_2bcoord: 129..295
e-value: 8.0E-18
score: 65.0
IPR036621Anticodon-binding domain superfamilyGENE3D3.40.50.800coord: 308..428
e-value: 3.9E-39
score: 135.2
IPR004154Anticodon-bindingPFAMPF03129HGTP_anticodoncoord: 314..410
e-value: 1.2E-18
score: 67.1
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 69..307
score: 18.88479
IPR033721Prolyl-tRNA synthetase, catalytic domainCDDcd00778ProRS_core_arch_eukcoord: 36..297
e-value: 2.67E-165
score: 466.302

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh14G014790.1CmaCh14G014790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006433 prolyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0017101 aminoacyl-tRNA synthetase multienzyme complex
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004827 proline-tRNA ligase activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding