CmaCh14G013570 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh14G013570
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Fatty acid desaturase family protein
Location	Cma_Chr14: 10551904 .. 10552486 (+)
RNA-Seq Expression	CmaCh14G013570
Synteny	CmaCh14G013570

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAATTTCCTCGGCAAATTCAATATGTCACATGTGGGAAAAACGACCGTGGAAAACAAACAATTTATCGGTATGTATCTTGAGTCAATCTTTAAATCAATTTGGAGTAAAATATGATAAACTTATTTATTGCATGAGCTTTTATTTCGTTTAATAATATTTTTCTTTTGTGTCTTAATTTCAATCCACAAAAATAGAATATTCAACTGTCCATTTTAAGATAGTGATTGGTGTCTTATCTATTAAGTTAGGTTGGTATGGACACACCTATCAAAATTACAAATATATTTTATCTAACTTACCTGCTTAGGTAACTGTGTATAGTCTTTCCATAATGGATCAACACTATATTAACCATAGAAAAATATTTTGCTATTCATTATCCTCCACAGGGTGGTGAATTTGCTTACATTTGGAGAAGGTTGGCACAACAATCACCATGCTTTTGAGTATTCAGCTAGGATGGGCATTGAATGGTGGCAATTTGACATGTTATTGTGTTTCTTGAAGCCATTGGAGTGGCTACTCATGTCAAATTGCCATCTCAAACCCTTATGCAAAAATTGGCCAAAGATTAACTAA

mRNA sequence

ATGCAATTTCCTCGGCAAATTCAATATGTCACATGTGGGAAAAACGACCGTGGAAAACAAACAATTTATCGGGTGGTGAATTTGCTTACATTTGGAGAAGGTTGGCACAACAATCACCATGCTTTTGAGTATTCAGCTAGGATGGGCATTGAATGGTGGCAATTTGACATGTTATTGTGTTTCTTGAAGCCATTGGAGTGGCTACTCATGTCAAATTGCCATCTCAAACCCTTATGCAAAAATTGGCCAAAGATTAACTAA

Coding sequence (CDS)

ATGCAATTTCCTCGGCAAATTCAATATGTCACATGTGGGAAAAACGACCGTGGAAAACAAACAATTTATCGGGTGGTGAATTTGCTTACATTTGGAGAAGGTTGGCACAACAATCACCATGCTTTTGAGTATTCAGCTAGGATGGGCATTGAATGGTGGCAATTTGACATGTTATTGTGTTTCTTGAAGCCATTGGAGTGGCTACTCATGTCAAATTGCCATCTCAAACCCTTATGCAAAAATTGGCCAAAGATTAACTAA

Protein sequence

MQFPRQIQYVTCGKNDRGKQTIYRVVNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLEWLLMSNCHLKPLCKNWPKIN

Homology

[Close]

BLAST of CmaCh14G013570 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 68.9 bits (167), Expect = 3.1e-11
Identity = 27/41 (65.85%), Postives = 33/41 (80.49%), Query Frame = 0


Query: 26  VNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLE 67
           V +LT GEGWHNNHHAFE+SAR G+EWWQ D+  C ++ LE
Sbjct: 299 VAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLE 339

[Close]

BLAST of CmaCh14G013570 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 4.1e-11
Identity = 29/43 (67.44%), Postives = 33/43 (76.74%), Query Frame = 0


Query: 26  VNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLEWL 69
           + +LT GEGWHNNHHAFEYSAR GIEWWQ D     +K LE+L
Sbjct: 249 IAMLTSGEGWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYL 291

[Close]

BLAST of CmaCh14G013570 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 5.3e-11
Identity = 29/43 (67.44%), Postives = 33/43 (76.74%), Query Frame = 0


Query: 26  VNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLEWL 69
           + +LT GEGWHNNHHAFEYSAR GIEWWQ D     +K LE+L
Sbjct: 246 IAMLTSGEGWHNNHHAFEYSARHGIEWWQIDTTWYVIKLLEYL 288

[Close]

BLAST of CmaCh14G013570 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 64.7 bits (156), Expect = 5.9e-10
Identity = 27/41 (65.85%), Postives = 31/41 (75.61%), Query Frame = 0


Query: 26  VNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLE 67
           V  L FGEGWHNNHHAFE+SAR G+EWWQ DM    +K L+
Sbjct: 307 VAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQ 347

[Close]

BLAST of CmaCh14G013570 vs. ExPASy Swiss-Prot
Match: Q9FV68 (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 8.5e-09
Identity = 24/41 (58.54%), Postives = 30/41 (73.17%), Query Frame = 0


Query: 26  VNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLE 67
           V L  FGEGWHNNHHAFE SAR G+EWW+ D+    ++ L+
Sbjct: 292 VALCAFGEGWHNNHHAFEQSARHGLEWWEIDVTWYVIRTLQ 332

[Close]

BLAST of CmaCh14G013570 vs. ExPASy TrEMBL
Match: A0A2U1QIP9 (Fatty acid desaturase family protein OS=Artemisia annua OX=35608 GN=CTI12_AA025160 PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 9.4e-11
Identity = 34/54 (62.96%), Postives = 41/54 (75.93%), Query Frame = 0


Query: 15  NDRGKQTIYRVVNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLEWL 69
           ND  K  ++  V LLTFGEGWHNNHHAFEYSAR+G+EWWQFD    F++ L+ L
Sbjct: 239 NDLSKNNMW--VALLTFGEGWHNNHHAFEYSARLGLEWWQFDPCWTFIQVLKAL 290

[Close]

BLAST of CmaCh14G013570 vs. ExPASy TrEMBL
Match: A0A563VKB5 (Fatty-acid desaturase OS=Hyella patelloides LEGE 07179 OX=945734 GN=H1P_1240008 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.6e-10
Identity = 38/67 (56.72%), Postives = 45/67 (67.16%), Query Frame = 0


Query: 15  NDRGKQTIYRVVNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLEWL-LMSNC 74
           NDR K   +  V LLTFGEGWHNNHHA++YSAR G+EWW+ D+    +K LE L L S  
Sbjct: 207 NDRSKNCWW--VALLTFGEGWHNNHHAYQYSARHGLEWWEIDITWMIIKTLETLGLASKI 266

Query: 75  HLKPLCK 81
            L PL K
Sbjct: 267 KLPPLQK 271

[Close]

BLAST of CmaCh14G013570 vs. ExPASy TrEMBL
Match: A0A6S7L973 (FA_desaturase domain-containing protein OS=Lactuca saligna OX=75948 GN=LSAL_LOCUS7945 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 3.6e-10
Identity = 30/41 (73.17%), Postives = 33/41 (80.49%), Query Frame = 0


Query: 26  VNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLE 67
           V L TFGEGWHNNHHAFEYSAR G+EWWQ D+  CF+  LE
Sbjct: 254 VALFTFGEGWHNNHHAFEYSARFGLEWWQLDLCWCFILFLE 294

[Close]

BLAST of CmaCh14G013570 vs. ExPASy TrEMBL
Match: A0A2J6MG83 (FA_desaturase domain-containing protein OS=Lactuca sativa OX=4236 GN=LSAT_1X33441 PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 3.6e-10
Identity = 30/41 (73.17%), Postives = 33/41 (80.49%), Query Frame = 0


Query: 26  VNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLE 67
           V L TFGEGWHNNHHAFEYSAR G+EWWQ D+  CF+  LE
Sbjct: 91  VALFTFGEGWHNNHHAFEYSARFGLEWWQLDLCWCFILFLE 131

[Close]

BLAST of CmaCh14G013570 vs. ExPASy TrEMBL
Match: A0A2U1QHX7 (Fatty acid desaturase family protein OS=Artemisia annua OX=35608 GN=CTI12_AA014460 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 4.7e-10
Identity = 31/43 (72.09%), Postives = 36/43 (83.72%), Query Frame = 0


Query: 26  VNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLEWL 69
           V LLTFGEGWHNNHHAFEYSAR+G+EWWQFD    F++ L+ L
Sbjct: 278 VALLTFGEGWHNNHHAFEYSARLGLEWWQFDPCWTFIQVLKAL 320

[Close]

BLAST of CmaCh14G013570 vs. NCBI nr
Match: PWA97848.1 (fatty acid desaturase family protein [Artemisia annua])

HSP 1 Score: 75.9 bits (185), Expect = 1.9e-10
Identity = 34/54 (62.96%), Postives = 41/54 (75.93%), Query Frame = 0


Query: 15  NDRGKQTIYRVVNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLEWL 69
           ND  K  ++  V LLTFGEGWHNNHHAFEYSAR+G+EWWQFD    F++ L+ L
Sbjct: 239 NDLSKNNMW--VALLTFGEGWHNNHHAFEYSARLGLEWWQFDPCWTFIQVLKAL 290

[Close]

BLAST of CmaCh14G013570 vs. NCBI nr
Match: KAG6581938.1 (Acyl-CoA C20 Delta5-desaturase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 75.5 bits (184), Expect = 2.5e-10
Identity = 37/64 (57.81%), Postives = 46/64 (71.88%), Query Frame = 0


Query: 25 VVNLLTFGEGWHNNHHAFEYSARMGIEWWQFD---MLLCFLKPL----EWLLMSNCHLKP 82
          VVNLL FGEGWH+NHHAFEYSARMGIEWWQFD    ++ FL+ +    +  L S  H++ 
Sbjct: 18 VVNLLLFGEGWHSNHHAFEYSARMGIEWWQFDPGWYVIVFLEAIGVATDVKLPSQTHMQK 77

[Close]

BLAST of CmaCh14G013570 vs. NCBI nr
Match: WP_144869683.1 (fatty acid desaturase [Hyella patelloides] >VEP11884.1 Fatty-acid desaturase [Hyella patelloides LEGE 07179])

HSP 1 Score: 75.1 bits (183), Expect = 3.3e-10
Identity = 38/67 (56.72%), Postives = 45/67 (67.16%), Query Frame = 0


Query: 15  NDRGKQTIYRVVNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLEWL-LMSNC 74
           NDR K   +  V LLTFGEGWHNNHHA++YSAR G+EWW+ D+    +K LE L L S  
Sbjct: 207 NDRSKNCWW--VALLTFGEGWHNNHHAYQYSARHGLEWWEIDITWMIIKTLETLGLASKI 266

Query: 75  HLKPLCK 81
            L PL K
Sbjct: 267 KLPPLQK 271

[Close]

BLAST of CmaCh14G013570 vs. NCBI nr
Match: PLY98606.1 (hypothetical protein LSAT_1X33441 [Lactuca sativa])

HSP 1 Score: 73.9 bits (180), Expect = 7.4e-10
Identity = 30/41 (73.17%), Postives = 33/41 (80.49%), Query Frame = 0


Query: 26  VNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLE 67
           V L TFGEGWHNNHHAFEYSAR G+EWWQ D+  CF+  LE
Sbjct: 91  VALFTFGEGWHNNHHAFEYSARFGLEWWQLDLCWCFILFLE 131

[Close]

BLAST of CmaCh14G013570 vs. NCBI nr
Match: CAB4079371.1 (unnamed protein product [Lactuca saligna])

HSP 1 Score: 73.9 bits (180), Expect = 7.4e-10
Identity = 30/41 (73.17%), Postives = 33/41 (80.49%), Query Frame = 0


Query: 26  VNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLE 67
           V L TFGEGWHNNHHAFEYSAR G+EWWQ D+  CF+  LE
Sbjct: 254 VALFTFGEGWHNNHHAFEYSARFGLEWWQLDLCWCFILFLE 294

[Close]

BLAST of CmaCh14G013570 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 68.9 bits (167), Expect = 2.2e-12
Identity = 27/41 (65.85%), Postives = 33/41 (80.49%), Query Frame = 0


Query: 26  VNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLE 67
           V +LT GEGWHNNHHAFE+SAR G+EWWQ D+  C ++ LE
Sbjct: 299 VAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLE 339

[Close]

BLAST of CmaCh14G013570 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 64.7 bits (156), Expect = 4.2e-11
Identity = 27/41 (65.85%), Postives = 31/41 (75.61%), Query Frame = 0


Query: 26  VNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLE 67
           V  L FGEGWHNNHHAFE+SAR G+EWWQ DM    +K L+
Sbjct: 307 VAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQ 347

[Close]

BLAST of CmaCh14G013570 vs. TAIR 10
Match: AT1G06360.1 (Fatty acid desaturase family protein )

HSP 1 Score: 59.3 bits (142), Expect = 1.7e-09
Identity = 24/43 (55.81%), Postives = 30/43 (69.77%), Query Frame = 0


Query: 26  VNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLEWL 69
           ++L T GE WHNNHHAFE SAR G+EWWQ D+    ++  E L
Sbjct: 236 LSLFTMGESWHNNHHAFESSARQGLEWWQIDITWYLIRLFEVL 278

[Close]

BLAST of CmaCh14G013570 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 59.3 bits (142), Expect = 1.7e-09
Identity = 23/41 (56.10%), Postives = 31/41 (75.61%), Query Frame = 0


Query: 26  VNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLE 67
           +++ +FGE WHNNHHAFE SAR G+EWWQ D+    ++ LE
Sbjct: 242 LSVFSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLE 282

[Close]

BLAST of CmaCh14G013570 vs. TAIR 10
Match: AT1G06350.1 (Fatty acid desaturase family protein )

HSP 1 Score: 59.3 bits (142), Expect = 1.7e-09
Identity = 24/43 (55.81%), Postives = 30/43 (69.77%), Query Frame = 0


Query: 26  VNLLTFGEGWHNNHHAFEYSARMGIEWWQFDMLLCFLKPLEWL 69
           ++L T GE WHNNHHAFE SAR G+EWWQ D+    ++  E L
Sbjct: 237 LSLFTMGESWHNNHHAFESSARQGLEWWQIDITWYLIRLFEVL 279

The following BLAST results are available for this feature:

BLAST of CmaCh14G013570 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LVZ3	3.1e-11	65.85	Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
P0DOW2	4.1e-11	67.44	Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1	[more]
P0DOW3	5.3e-11	67.44	Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1	[more]
Q949X0	5.9e-10	65.85	Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab...	[more]
Q9FV68	8.5e-09	58.54	Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii OX=28973 PE=1 SV=1	[more]

BLAST of CmaCh14G013570 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2U1QIP9	9.4e-11	62.96	Fatty acid desaturase family protein OS=Artemisia annua OX=35608 GN=CTI12_AA0251...	[more]
A0A563VKB5	1.6e-10	56.72	Fatty-acid desaturase OS=Hyella patelloides LEGE 07179 OX=945734 GN=H1P_1240008 ...	[more]
A0A6S7L973	3.6e-10	73.17	FA_desaturase domain-containing protein OS=Lactuca saligna OX=75948 GN=LSAL_LOCU...	[more]
A0A2J6MG83	3.6e-10	73.17	FA_desaturase domain-containing protein OS=Lactuca sativa OX=4236 GN=LSAT_1X3344...	[more]
A0A2U1QHX7	4.7e-10	72.09	Fatty acid desaturase family protein OS=Artemisia annua OX=35608 GN=CTI12_AA0144...	[more]

BLAST of CmaCh14G013570 vs. NCBI nr


Match Name	E-value	Identity	Description
PWA97848.1	1.9e-10	62.96	fatty acid desaturase family protein [Artemisia annua]	[more]
KAG6581938.1	2.5e-10	57.81	Acyl-CoA C20 Delta5-desaturase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
WP_144869683.1	3.3e-10	56.72	fatty acid desaturase [Hyella patelloides] >VEP11884.1 Fatty-acid desaturase [Hy...	[more]
PLY98606.1	7.4e-10	73.17	hypothetical protein LSAT_1X33441 [Lactuca sativa]	[more]
CAB4079371.1	7.4e-10	73.17	unnamed protein product [Lactuca saligna]	[more]

BLAST of CmaCh14G013570 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15870.1	2.2e-12	65.85	Fatty acid desaturase family protein	[more]
AT3G15850.1	4.2e-11	65.85	fatty acid desaturase 5	[more]
AT1G06360.1	1.7e-09	55.81	Fatty acid desaturase family protein	[more]
AT1G06080.1	1.7e-09	56.10	delta 9 desaturase 1	[more]
AT1G06350.1	1.7e-09	55.81	Fatty acid desaturase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015876	Acyl-CoA desaturase	PANTHER	PTHR11351	ACYL-COA DESATURASE	coord: 24..68
None	No IPR available	PANTHER	PTHR11351:SF87	FATTY ACID DESATURASE, TYPE 1, CORE-RELATED	coord: 24..68

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh14G013570.1	CmaCh14G013570.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009979	16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water