CmaCh14G013470 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh14G013470
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	26.5 kDa heat shock protein, mitochondrial
Location	Cma_Chr14: 10487066 .. 10487698 (+)
RNA-Seq Expression	CmaCh14G013470
Synteny	CmaCh14G013470

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCCTGACTCGATTGGCTCTGAAGAATTTGCAGCAAAGGGCGCTCGTTTCCTCTCCTGCTGATTCTCTGCTCCACAGGCGAACAGAGGCCCTGATCAGAAGGTTCTGCGATCAGAGCTCAGAAGGCAATCCCAAAGAAGTGGCCATTAATAATGAAGACAAAAACACCAAAATGGTCCCGAAAGGGGAAGGCAAGAAGTGGGTATTATGGAAGAAGAACGGGAGAGAATTTGATGTGGGGAATTCACTGATGCAAGCGGCTGAGAACATAAACAGGGTATTGAAGAGCTTGAAGTTGAGGCGTCCATGGTGGGTATCCAGTGGGCATGTGAAAGAGCAAGAAGAGTGGTACAAGCTGAGGGTGGAGATGCCTGGGATTGGGAAGGAGGACGTGAAGGTGAGGGTGGAGGGTCGGATTCTGAGCATCAGAGGGGAGCACAGTGAAGAAGATGAAGAAGAAGAAAGTGGGTGGAGTGGGGAGAGATATGGGTACTATGAGAGCACGGTGATGCTGCCGGAGGACGCGGTGGCGGATGAGATCAAGGCGGAGCTGAAAGATGGGGTGTTGACGATCACCATTCCAAGGACTGAGATGCCCCCAAAGGATGTTAAAGAGATCACCGTCGTGTAA

mRNA sequence

ATGGCCCTGACTCGATTGGCTCTGAAGAATTTGCAGCAAAGGGCGCTCGTTTCCTCTCCTGCTGATTCTCTGCTCCACAGGCGAACAGAGGCCCTGATCAGAAGGTTCTGCGATCAGAGCTCAGAAGGCAATCCCAAAGAAGTGGCCATTAATAATGAAGACAAAAACACCAAAATGGTCCCGAAAGGGGAAGGCAAGAAGTGGGTATTATGGAAGAAGAACGGGAGAGAATTTGATGTGGGGAATTCACTGATGCAAGCGGCTGAGAACATAAACAGGGTATTGAAGAGCTTGAAGTTGAGGCGTCCATGGTGGGTATCCAGTGGGCATGTGAAAGAGCAAGAAGAGTGGTACAAGCTGAGGGTGGAGATGCCTGGGATTGGGAAGGAGGACGTGAAGGTGAGGGTGGAGGGTCGGATTCTGAGCATCAGAGGGGAGCACAGTGAAGAAGATGAAGAAGAAGAAAGTGGGTGGAGTGGGGAGAGATATGGGTACTATGAGAGCACGGTGATGCTGCCGGAGGACGCGGTGGCGGATGAGATCAAGGCGGAGCTGAAAGATGGGGTGTTGACGATCACCATTCCAAGGACTGAGATGCCCCCAAAGGATGTTAAAGAGATCACCGTCGTGTAA

Coding sequence (CDS)

ATGGCCCTGACTCGATTGGCTCTGAAGAATTTGCAGCAAAGGGCGCTCGTTTCCTCTCCTGCTGATTCTCTGCTCCACAGGCGAACAGAGGCCCTGATCAGAAGGTTCTGCGATCAGAGCTCAGAAGGCAATCCCAAAGAAGTGGCCATTAATAATGAAGACAAAAACACCAAAATGGTCCCGAAAGGGGAAGGCAAGAAGTGGGTATTATGGAAGAAGAACGGGAGAGAATTTGATGTGGGGAATTCACTGATGCAAGCGGCTGAGAACATAAACAGGGTATTGAAGAGCTTGAAGTTGAGGCGTCCATGGTGGGTATCCAGTGGGCATGTGAAAGAGCAAGAAGAGTGGTACAAGCTGAGGGTGGAGATGCCTGGGATTGGGAAGGAGGACGTGAAGGTGAGGGTGGAGGGTCGGATTCTGAGCATCAGAGGGGAGCACAGTGAAGAAGATGAAGAAGAAGAAAGTGGGTGGAGTGGGGAGAGATATGGGTACTATGAGAGCACGGTGATGCTGCCGGAGGACGCGGTGGCGGATGAGATCAAGGCGGAGCTGAAAGATGGGGTGTTGACGATCACCATTCCAAGGACTGAGATGCCCCCAAAGGATGTTAAAGAGATCACCGTCGTGTAA

Protein sequence

MALTRLALKNLQQRALVSSPADSLLHRRTEALIRRFCDQSSEGNPKEVAINNEDKNTKMVPKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSSGHVKEQEEWYKLRVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWSGERYGYYESTVMLPEDAVADEIKAELKDGVLTITIPRTEMPPKDVKEITVV

Homology

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BLAST of CmaCh14G013470 vs. ExPASy Swiss-Prot
Match: Q9SSQ8 (26.5 kDa heat shock protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HSP26.5 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.0e-32
Identity = 95/234 (40.60%), Postives = 140/234 (59.83%), Query Frame = 0


Query: 1   MALTRLALKNLQQRALVSSPADS-----LLHRRTEALIRRFCDQSS-EGNPK---EVAIN 60
           MAL RLAL+NLQQ+   S    S     + +R     + RF   S+ E   K   EV+++
Sbjct: 1   MALARLALRNLQQKLSPSLMGQSCERGLVGNRHNPMKLNRFMATSAGEQEDKMNTEVSVS 60

Query: 61  NEDKNTKMVPKGEGKKWVLWKKNG---------REF---DVGNSLMQAAENINRVLKSLK 120
            +    +  P+  G+K  LW+             EF    +GN+L+QA EN+NR+  +  
Sbjct: 61  EKKSPRQNFPRRRGRK-SLWRNTDDHGYFTPTLNEFFPPTIGNTLIQATENMNRIFDNFN 120

Query: 121 LRRPWWVSSGHVKEQEEWYKLRVEMPGIGKEDVKVRVEGRILSIRGEHSEEDE----EEE 180
           +  P+ +  G VKEQ++ YKLR E+PG+ KEDVK+ V   IL+I+G+H  E+E    EE+
Sbjct: 121 V-NPFQL-MGQVKEQDDCYKLRYEVPGLTKEDVKITVNDGILTIKGDHKAEEEKGSPEED 180

Query: 181 SGWSGERYGYYESTVMLPEDAVADEIKAELKDGVLTITIPRTEMPPKDVKEITV 210
             WS + YGYY +++ LP+DA  ++IKAELK+GVL + IPRTE P K+V+EI+V
Sbjct: 181 EYWSSKSYGYYNTSLSLPDDAKVEDIKAELKNGVLNLVIPRTEKPKKNVQEISV 231

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BLAST of CmaCh14G013470 vs. ExPASy Swiss-Prot
Match: Q95661 (Small heat shock protein, chloroplastic OS=Solanum lycopersicum OX=4081 GN=HSP21 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 7.1e-17
Identity = 50/112 (44.64%), Postives = 72/112 (64.29%), Query Frame = 0


Query: 92  NRVLKSLKLRRPWWVSSGHVKEQEEWYKLRVEMPGIGKEDVKVRVEGRILSIRGEH-SEE 151
           NR   + ++R PW      + + E   K+R +MPG+ KEDVKV VE  +L I+GEH  EE
Sbjct: 123 NRASGTGEIRTPW-----DIHDDENEIKMRFDMPGLSKEDVKVSVENDMLVIKGEHKKEE 182

Query: 152 DEEEESGWSGERYGYYESTVMLPEDAVADEIKAELKDGVLTITIPRTEMPPK 203
           D  ++  W G  Y  Y++ + LP++ V D+IKAELK+GVL I+IP+TE+  K
Sbjct: 183 DGRDKHSW-GRNYSSYDTRLSLPDNVVKDKIKAELKNGVLFISIPKTEVEKK 228

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BLAST of CmaCh14G013470 vs. ExPASy Swiss-Prot
Match: P31170 (Heat shock protein 21, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HSP21 PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.2e-16
Identity = 46/104 (44.23%), Postives = 68/104 (65.38%), Query Frame = 0


Query: 99  KLRRPWWVSSGHVKEQEEWYKLRVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGW 158
           ++R PW      +KE+E   K+R +MPG+ KEDVK+ VE  +L I+GE  +ED ++   W
Sbjct: 124 EIRAPW-----DIKEEEHEIKMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDD--SW 183

Query: 159 SGERYGYYESTVMLPEDAVADEIKAELKDGVLTITIPRTEMPPK 203
           SG     Y + + LP++   D+IKAELK+GVL ITIP+T++  K
Sbjct: 184 SGRSVSSYGTRLQLPDNCEKDKIKAELKNGVLFITIPKTKVERK 220

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BLAST of CmaCh14G013470 vs. ExPASy Swiss-Prot
Match: B7EZJ7 (23.6 kDa heat shock protein, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=HSP23.6 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.7e-16
Identity = 53/134 (39.55%), Postives = 81/134 (60.45%), Query Frame = 0


Query: 80  VGNSLMQAAENINRVLKSLKLRRPWWVSSGHVKEQEEWYKLRVEMPGIGKEDVKVRVEGR 139
           +G++L Q AE + R L            +G V+E EE Y+LR E+PG+GK+DV+V V+  
Sbjct: 98  IGSALSQVAETLTRPL------------TGKVREDEERYRLRFEVPGLGKDDVRVYVDDG 157

Query: 140 ILSIRGEHSEEDEEEESGWSGE----RYGYYESTVMLPEDAVADEIKAELKDGVLTITIP 199
           +L+I GE   +  EE+ G  G+        Y + ++LPEDAVA+ I AE++DGVL +T+P
Sbjct: 158 VLAIHGE-KRDVVEEDRGRDGDGECWAAATYHAGLLLPEDAVAEGITAEVRDGVLHVTVP 217

Query: 200 RTEMPPKDVKEITV 210
           R+    + V E+ V
Sbjct: 218 RSPERKRSVTEVKV 218

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BLAST of CmaCh14G013470 vs. ExPASy Swiss-Prot
Match: P30222 (Small heat shock protein, chloroplastic OS=Petunia hybrida OX=4102 GN=HSP22 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 4.6e-16
Identity = 43/104 (41.35%), Postives = 69/104 (66.35%), Query Frame = 0


Query: 99  KLRRPWWVSSGHVKEQEEWYKLRVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGW 158
           ++R PW      +K+ E   K+R +MPG+ KE+VKV VE  +L I+GEH +E+  ++  W
Sbjct: 137 EIRAPW-----DIKDDENEIKMRFDMPGLSKEEVKVSVEDDVLVIKGEHKKEESGKDDSW 196

Query: 159 SGERYGYYESTVMLPEDAVADEIKAELKDGVLTITIPRTEMPPK 203
            G  Y  Y++ + LP++   D++KAELK+GVL I+IP+T++  K
Sbjct: 197 -GRNYSSYDTRLSLPDNVDKDKVKAELKNGVLLISIPKTKVEKK 234

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BLAST of CmaCh14G013470 vs. ExPASy TrEMBL
Match: A0A6J1IQQ6 (26.5 kDa heat shock protein, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111479146 PE=3 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 2.1e-112
Identity = 210/210 (100.00%), Postives = 210/210 (100.00%), Query Frame = 0


Query: 1   MALTRLALKNLQQRALVSSPADSLLHRRTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 60
           MALTRLALKNLQQRALVSSPADSLLHRRTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV
Sbjct: 1   MALTRLALKNLQQRALVSSPADSLLHRRTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 60

Query: 61  PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSSGHVKEQEEWYKL 120
           PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSSGHVKEQEEWYKL
Sbjct: 61  PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSSGHVKEQEEWYKL 120

Query: 121 RVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWSGERYGYYESTVMLPEDAVADE 180
           RVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWSGERYGYYESTVMLPEDAVADE
Sbjct: 121 RVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWSGERYGYYESTVMLPEDAVADE 180

Query: 181 IKAELKDGVLTITIPRTEMPPKDVKEITVV 211
           IKAELKDGVLTITIPRTEMPPKDVKEITVV
Sbjct: 181 IKAELKDGVLTITIPRTEMPPKDVKEITVV 210

[Close]

BLAST of CmaCh14G013470 vs. ExPASy TrEMBL
Match: A0A6J1GVM4 (26.5 kDa heat shock protein, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457576 PE=3 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 5.9e-107
Identity = 201/210 (95.71%), Postives = 204/210 (97.14%), Query Frame = 0


Query: 1   MALTRLALKNLQQRALVSSPADSLLHRRTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 60
           MALTRLALKNLQQRALVSS AD+LLHR+TEALIRRFCDQSSEGNPKEVAINNEDKNTKMV
Sbjct: 80  MALTRLALKNLQQRALVSSSADALLHRQTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 139

Query: 61  PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSSGHVKEQEEWYKL 120
           PKGEGK+WVLWKKNGREFDVGNSLMQAAENINRVLKSL LRRPWWVS GHVKEQEEWYKL
Sbjct: 140 PKGEGKRWVLWKKNGREFDVGNSLMQAAENINRVLKSLNLRRPWWVSGGHVKEQEEWYKL 199

Query: 121 RVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWSGERYGYYESTVMLPEDAVADE 180
           RVEMPGI KEDVKVRVEGRILSIRGEHSEEDEEEESGW G RYGYYESTVMLPEDAVADE
Sbjct: 200 RVEMPGIRKEDVKVRVEGRILSIRGEHSEEDEEEESGWRGGRYGYYESTVMLPEDAVADE 259

Query: 181 IKAELKDGVLTITIPRTEMPPKDVKEITVV 211
           IKAELKDGVLTITIPRTEMPPKDVKEITVV
Sbjct: 260 IKAELKDGVLTITIPRTEMPPKDVKEITVV 289

[Close]

BLAST of CmaCh14G013470 vs. ExPASy TrEMBL
Match: A0A6J1GUB2 (26.5 kDa heat shock protein, mitochondrial isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457576 PE=3 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 5.9e-107
Identity = 201/210 (95.71%), Postives = 204/210 (97.14%), Query Frame = 0


Query: 1   MALTRLALKNLQQRALVSSPADSLLHRRTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 60
           MALTRLALKNLQQRALVSS AD+LLHR+TEALIRRFCDQSSEGNPKEVAINNEDKNTKMV
Sbjct: 60  MALTRLALKNLQQRALVSSSADALLHRQTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 119

Query: 61  PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSSGHVKEQEEWYKL 120
           PKGEGK+WVLWKKNGREFDVGNSLMQAAENINRVLKSL LRRPWWVS GHVKEQEEWYKL
Sbjct: 120 PKGEGKRWVLWKKNGREFDVGNSLMQAAENINRVLKSLNLRRPWWVSGGHVKEQEEWYKL 179

Query: 121 RVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWSGERYGYYESTVMLPEDAVADE 180
           RVEMPGI KEDVKVRVEGRILSIRGEHSEEDEEEESGW G RYGYYESTVMLPEDAVADE
Sbjct: 180 RVEMPGIRKEDVKVRVEGRILSIRGEHSEEDEEEESGWRGGRYGYYESTVMLPEDAVADE 239

Query: 181 IKAELKDGVLTITIPRTEMPPKDVKEITVV 211
           IKAELKDGVLTITIPRTEMPPKDVKEITVV
Sbjct: 240 IKAELKDGVLTITIPRTEMPPKDVKEITVV 269

[Close]

BLAST of CmaCh14G013470 vs. ExPASy TrEMBL
Match: A0A5A7TMA9 (26.5 kDa heat shock protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001750 PE=3 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 1.1e-84
Identity = 164/211 (77.73%), Postives = 184/211 (87.20%), Query Frame = 0


Query: 1   MALTRLALKNLQQR-ALVSSPADSLLHRRTEALIRRFCDQSSEGNPKEVAINNEDKNTKM 60
           MALTRLAL+NLQQR A VSSP D+LL ++T ALIRRFCDQSSEGN KE+A+ NE+KNTK+
Sbjct: 1   MALTRLALRNLQQRAAFVSSPVDTLLPKQTAALIRRFCDQSSEGNSKEIAVKNEEKNTKI 60

Query: 61  VPKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSSGHVKEQEEWYK 120
            PK EGKKW+ W+KN  EF +GNSLMQAAE+INRVLKSL L RPWW S GHVKEQE+WYK
Sbjct: 61  APKREGKKWIPWRKNEGEFHLGNSLMQAAEHINRVLKSLNLSRPWWFSGGHVKEQEDWYK 120

Query: 121 LRVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWSGERYGYYESTVMLPEDAVAD 180
           LR+EMPGI K++VKV VEGR+L+IRGEH EE+EEEE     E YGYYESTVMLPEDAVA+
Sbjct: 121 LRMEMPGIAKDEVKVTVEGRMLNIRGEHREEEEEEEEEGFAESYGYYESTVMLPEDAVAE 180

Query: 181 EIKAELKDGVLTITIPRTEMPPKDVKEITVV 211
           EIKAELKDGVLTITIPRTE PPKDVKEITVV
Sbjct: 181 EIKAELKDGVLTITIPRTEKPPKDVKEITVV 211

[Close]

BLAST of CmaCh14G013470 vs. ExPASy TrEMBL
Match: A0A1S3BYA0 (26.5 kDa heat shock protein, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103494889 PE=3 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 1.1e-84
Identity = 164/211 (77.73%), Postives = 184/211 (87.20%), Query Frame = 0


Query: 1   MALTRLALKNLQQR-ALVSSPADSLLHRRTEALIRRFCDQSSEGNPKEVAINNEDKNTKM 60
           MALTRLAL+NLQQR A VSSP D+LL ++T ALIRRFCDQSSEGN KE+A+ NE+KNTK+
Sbjct: 1   MALTRLALRNLQQRAAFVSSPVDTLLPKQTAALIRRFCDQSSEGNSKEIAVKNEEKNTKI 60

Query: 61  VPKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSSGHVKEQEEWYK 120
            PK EGKKW+ W+KN  EF +GNSLMQAAE+INRVLKSL L RPWW S GHVKEQE+WYK
Sbjct: 61  APKREGKKWIPWRKNEGEFHLGNSLMQAAEHINRVLKSLNLSRPWWFSGGHVKEQEDWYK 120

Query: 121 LRVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWSGERYGYYESTVMLPEDAVAD 180
           LR+EMPGI K++VKV VEGR+L+IRGEH EE+EEEE     E YGYYESTVMLPEDAVA+
Sbjct: 121 LRMEMPGIAKDEVKVTVEGRMLNIRGEHREEEEEEEEEGFAESYGYYESTVMLPEDAVAE 180

Query: 181 EIKAELKDGVLTITIPRTEMPPKDVKEITVV 211
           EIKAELKDGVLTITIPRTE PPKDVKEITVV
Sbjct: 181 EIKAELKDGVLTITIPRTEKPPKDVKEITVV 211

[Close]

BLAST of CmaCh14G013470 vs. NCBI nr
Match: XP_022979416.1 (26.5 kDa heat shock protein, mitochondrial [Cucurbita maxima])

HSP 1 Score: 414.8 bits (1065), Expect = 4.3e-112
Identity = 210/210 (100.00%), Postives = 210/210 (100.00%), Query Frame = 0


Query: 1   MALTRLALKNLQQRALVSSPADSLLHRRTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 60
           MALTRLALKNLQQRALVSSPADSLLHRRTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV
Sbjct: 1   MALTRLALKNLQQRALVSSPADSLLHRRTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 60

Query: 61  PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSSGHVKEQEEWYKL 120
           PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSSGHVKEQEEWYKL
Sbjct: 61  PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSSGHVKEQEEWYKL 120

Query: 121 RVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWSGERYGYYESTVMLPEDAVADE 180
           RVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWSGERYGYYESTVMLPEDAVADE
Sbjct: 121 RVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWSGERYGYYESTVMLPEDAVADE 180

Query: 181 IKAELKDGVLTITIPRTEMPPKDVKEITVV 211
           IKAELKDGVLTITIPRTEMPPKDVKEITVV
Sbjct: 181 IKAELKDGVLTITIPRTEMPPKDVKEITVV 210

[Close]

BLAST of CmaCh14G013470 vs. NCBI nr
Match: XP_023526860.1 (LOW QUALITY PROTEIN: 26.5 kDa heat shock protein, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 401.4 bits (1030), Expect = 4.9e-108
Identity = 203/210 (96.67%), Postives = 207/210 (98.57%), Query Frame = 0


Query: 1   MALTRLALKNLQQRALVSSPADSLLHRRTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 60
           MALTRLALKNLQQRALVSSPAD+LLHR+TEALIRRFCDQSSEGNPKEVAINNEDKNTKMV
Sbjct: 1   MALTRLALKNLQQRALVSSPADALLHRKTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 60

Query: 61  PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSSGHVKEQEEWYKL 120
           PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVS+GHVKEQEE YKL
Sbjct: 61  PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSNGHVKEQEEXYKL 120

Query: 121 RVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWSGERYGYYESTVMLPEDAVADE 180
           +VEMPGI KEDVKVRVEGRILSIRGEHSEEDEEEESGW GERYGYYESTVMLPEDAVADE
Sbjct: 121 KVEMPGIAKEDVKVRVEGRILSIRGEHSEEDEEEESGWRGERYGYYESTVMLPEDAVADE 180

Query: 181 IKAELKDGVLTITIPRTEMPPKDVKEITVV 211
           IKAELKDGVLTITIPRTEMPPKDVKEITVV
Sbjct: 181 IKAELKDGVLTITIPRTEMPPKDVKEITVV 210

[Close]

BLAST of CmaCh14G013470 vs. NCBI nr
Match: KAG6581923.1 (26.5 kDa heat shock protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 399.8 bits (1026), Expect = 1.4e-107
Identity = 202/210 (96.19%), Postives = 206/210 (98.10%), Query Frame = 0


Query: 1   MALTRLALKNLQQRALVSSPADSLLHRRTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 60
           MALTRLALKNLQQRALVSS AD+LLHR+TEALIRRFCDQSSEGNPKEVAINNEDKNTKMV
Sbjct: 80  MALTRLALKNLQQRALVSSSADALLHRQTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 139

Query: 61  PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSSGHVKEQEEWYKL 120
           PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVS+GHVKEQEEWYKL
Sbjct: 140 PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSNGHVKEQEEWYKL 199

Query: 121 RVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWSGERYGYYESTVMLPEDAVADE 180
           +VEMPGI KEDVKVRVEGRILSIRGEH EEDEEEESGW GERYGYYESTVMLPEDAVADE
Sbjct: 200 KVEMPGIRKEDVKVRVEGRILSIRGEHREEDEEEESGWRGERYGYYESTVMLPEDAVADE 259

Query: 181 IKAELKDGVLTITIPRTEMPPKDVKEITVV 211
           IKAELKDGVLTITIPRTEMPPKDVKEITVV
Sbjct: 260 IKAELKDGVLTITIPRTEMPPKDVKEITVV 289

[Close]

BLAST of CmaCh14G013470 vs. NCBI nr
Match: XP_022955630.1 (26.5 kDa heat shock protein, mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 396.7 bits (1018), Expect = 1.2e-106
Identity = 201/210 (95.71%), Postives = 204/210 (97.14%), Query Frame = 0


Query: 1   MALTRLALKNLQQRALVSSPADSLLHRRTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 60
           MALTRLALKNLQQRALVSS AD+LLHR+TEALIRRFCDQSSEGNPKEVAINNEDKNTKMV
Sbjct: 80  MALTRLALKNLQQRALVSSSADALLHRQTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 139

Query: 61  PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSSGHVKEQEEWYKL 120
           PKGEGK+WVLWKKNGREFDVGNSLMQAAENINRVLKSL LRRPWWVS GHVKEQEEWYKL
Sbjct: 140 PKGEGKRWVLWKKNGREFDVGNSLMQAAENINRVLKSLNLRRPWWVSGGHVKEQEEWYKL 199

Query: 121 RVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWSGERYGYYESTVMLPEDAVADE 180
           RVEMPGI KEDVKVRVEGRILSIRGEHSEEDEEEESGW G RYGYYESTVMLPEDAVADE
Sbjct: 200 RVEMPGIRKEDVKVRVEGRILSIRGEHSEEDEEEESGWRGGRYGYYESTVMLPEDAVADE 259

Query: 181 IKAELKDGVLTITIPRTEMPPKDVKEITVV 211
           IKAELKDGVLTITIPRTEMPPKDVKEITVV
Sbjct: 260 IKAELKDGVLTITIPRTEMPPKDVKEITVV 289

[Close]

BLAST of CmaCh14G013470 vs. NCBI nr
Match: XP_022955631.1 (26.5 kDa heat shock protein, mitochondrial isoform X2 [Cucurbita moschata])

HSP 1 Score: 396.7 bits (1018), Expect = 1.2e-106
Identity = 201/210 (95.71%), Postives = 204/210 (97.14%), Query Frame = 0


Query: 1   MALTRLALKNLQQRALVSSPADSLLHRRTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 60
           MALTRLALKNLQQRALVSS AD+LLHR+TEALIRRFCDQSSEGNPKEVAINNEDKNTKMV
Sbjct: 60  MALTRLALKNLQQRALVSSSADALLHRQTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 119

Query: 61  PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSSGHVKEQEEWYKL 120
           PKGEGK+WVLWKKNGREFDVGNSLMQAAENINRVLKSL LRRPWWVS GHVKEQEEWYKL
Sbjct: 120 PKGEGKRWVLWKKNGREFDVGNSLMQAAENINRVLKSLNLRRPWWVSGGHVKEQEEWYKL 179

Query: 121 RVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWSGERYGYYESTVMLPEDAVADE 180
           RVEMPGI KEDVKVRVEGRILSIRGEHSEEDEEEESGW G RYGYYESTVMLPEDAVADE
Sbjct: 180 RVEMPGIRKEDVKVRVEGRILSIRGEHSEEDEEEESGWRGGRYGYYESTVMLPEDAVADE 239

Query: 181 IKAELKDGVLTITIPRTEMPPKDVKEITVV 211
           IKAELKDGVLTITIPRTEMPPKDVKEITVV
Sbjct: 240 IKAELKDGVLTITIPRTEMPPKDVKEITVV 269

[Close]

BLAST of CmaCh14G013470 vs. TAIR 10
Match: AT1G52560.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 140.6 bits (353), Expect = 1.5e-33
Identity = 95/234 (40.60%), Postives = 140/234 (59.83%), Query Frame = 0


Query: 1   MALTRLALKNLQQRALVSSPADS-----LLHRRTEALIRRFCDQSS-EGNPK---EVAIN 60
           MAL RLAL+NLQQ+   S    S     + +R     + RF   S+ E   K   EV+++
Sbjct: 1   MALARLALRNLQQKLSPSLMGQSCERGLVGNRHNPMKLNRFMATSAGEQEDKMNTEVSVS 60

Query: 61  NEDKNTKMVPKGEGKKWVLWKKNG---------REF---DVGNSLMQAAENINRVLKSLK 120
            +    +  P+  G+K  LW+             EF    +GN+L+QA EN+NR+  +  
Sbjct: 61  EKKSPRQNFPRRRGRK-SLWRNTDDHGYFTPTLNEFFPPTIGNTLIQATENMNRIFDNFN 120

Query: 121 LRRPWWVSSGHVKEQEEWYKLRVEMPGIGKEDVKVRVEGRILSIRGEHSEEDE----EEE 180
           +  P+ +  G VKEQ++ YKLR E+PG+ KEDVK+ V   IL+I+G+H  E+E    EE+
Sbjct: 121 V-NPFQL-MGQVKEQDDCYKLRYEVPGLTKEDVKITVNDGILTIKGDHKAEEEKGSPEED 180

Query: 181 SGWSGERYGYYESTVMLPEDAVADEIKAELKDGVLTITIPRTEMPPKDVKEITV 210
             WS + YGYY +++ LP+DA  ++IKAELK+GVL + IPRTE P K+V+EI+V
Sbjct: 181 EYWSSKSYGYYNTSLSLPDDAKVEDIKAELKNGVLNLVIPRTEKPKKNVQEISV 231

[Close]

BLAST of CmaCh14G013470 vs. TAIR 10
Match: AT1G52560.2 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 139.0 bits (349), Expect = 4.2e-33
Identity = 93/227 (40.97%), Postives = 137/227 (60.35%), Query Frame = 0


Query: 1   MALTRLALKNLQQRALVSSPADS-----LLHRRTEALIRRFCDQSS-EGNPK---EVAIN 60
           MAL RLAL+NLQQ+   S    S     + +R     + RF   S+ E   K   EV+++
Sbjct: 1   MALARLALRNLQQKLSPSLMGQSCERGLVGNRHNPMKLNRFMATSAGEQEDKMNTEVSVS 60

Query: 61  NEDKNTKMVPKGEGKKWVLWKKNGREFDV-----GNSLMQAAENINRVLKSLKLRRPWWV 120
            +    +  P+  G+K  LW+             GN+L+QA EN+NR+  +  +  P+ +
Sbjct: 61  EKKSPRQNFPRRRGRK-SLWRNTDDHGYFTPTLNGNTLIQATENMNRIFDNFNV-NPFQL 120

Query: 121 SSGHVKEQEEWYKLRVEMPGIGKEDVKVRVEGRILSIRGEHSEEDE----EEESGWSGER 180
             G VKEQ++ YKLR E+PG+ KEDVK+ V   IL+I+G+H  E+E    EE+  WS + 
Sbjct: 121 -MGQVKEQDDCYKLRYEVPGLTKEDVKITVNDGILTIKGDHKAEEEKGSPEEDEYWSSKS 180

Query: 181 YGYYESTVMLPEDAVADEIKAELKDGVLTITIPRTEMPPKDVKEITV 210
           YGYY +++ LP+DA  ++IKAELK+GVL + IPRTE P K+V+EI+V
Sbjct: 181 YGYYNTSLSLPDDAKVEDIKAELKNGVLNLVIPRTEKPKKNVQEISV 224

[Close]

BLAST of CmaCh14G013470 vs. TAIR 10
Match: AT4G27670.1 (heat shock protein 21 )

HSP 1 Score: 88.2 bits (217), Expect = 8.6e-18
Identity = 46/104 (44.23%), Postives = 68/104 (65.38%), Query Frame = 0


Query: 99  KLRRPWWVSSGHVKEQEEWYKLRVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGW 158
           ++R PW      +KE+E   K+R +MPG+ KEDVK+ VE  +L I+GE  +ED ++   W
Sbjct: 124 EIRAPW-----DIKEEEHEIKMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDD--SW 183

Query: 159 SGERYGYYESTVMLPEDAVADEIKAELKDGVLTITIPRTEMPPK 203
           SG     Y + + LP++   D+IKAELK+GVL ITIP+T++  K
Sbjct: 184 SGRSVSSYGTRLQLPDNCEKDKIKAELKNGVLFITIPKTKVERK 220

[Close]

BLAST of CmaCh14G013470 vs. TAIR 10
Match: AT4G25200.1 (mitochondrion-localized small heat shock protein 23.6 )

HSP 1 Score: 68.6 bits (166), Expect = 7.0e-12
Identity = 45/111 (40.54%), Postives = 67/111 (60.36%), Query Frame = 0


Query: 101 RRPWWVSSGHVKEQEEWYKLRVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWSG 160
           RR W      +KE+++   LR++MPG+ +EDVK+ +E   L IRGE   E++  E G SG
Sbjct: 106 RRGW-----DIKEKDDALYLRIDMPGLSREDVKLALEQDTLVIRGEGKNEEDGGEEGESG 165

Query: 161 ERYGYYESTVMLPEDAV-ADEIKAELKDGVLTITIPR-TEMPPKDVKEITV 210
            R   + S + LP+     DEIKAE+K+GVL + IP+  E    DV++I +
Sbjct: 166 NR--RFTSRIGLPDKIYKIDEIKAEMKNGVLKVVIPKMKEQERNDVRQIEI 209

[Close]

BLAST of CmaCh14G013470 vs. TAIR 10
Match: AT5G51440.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.2e-11
Identity = 43/98 (43.88%), Postives = 62/98 (63.27%), Query Frame = 0


Query: 100 LRRPWWVSSGHVKEQEEWYKLRVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWS 159
           +RR W     +VKE+++   LR++MPG+ +EDVK+ +E   L IRGE   E+ E+ SG  
Sbjct: 107 VRRGW-----NVKEKDDALHLRIDMPGLSREDVKLALEQNTLVIRGEGETEEGEDVSG-D 166

Query: 160 GERYGYYESTVMLPEDAV-ADEIKAELKDGVLTITIPR 197
           G R   + S + LPE     DEIKAE+K+GVL + IP+
Sbjct: 167 GRR---FTSRIELPEKVYKTDEIKAEMKNGVLKVVIPK 195

The following BLAST results are available for this feature:

BLAST of CmaCh14G013470 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SSQ8	2.0e-32	40.60	26.5 kDa heat shock protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HS...	[more]
Q95661	7.1e-17	44.64	Small heat shock protein, chloroplastic OS=Solanum lycopersicum OX=4081 GN=HSP21...	[more]
P31170	1.2e-16	44.23	Heat shock protein 21, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HSP21 PE...	[more]
B7EZJ7	2.7e-16	39.55	23.6 kDa heat shock protein, mitochondrial OS=Oryza sativa subsp. japonica OX=39...	[more]
P30222	4.6e-16	41.35	Small heat shock protein, chloroplastic OS=Petunia hybrida OX=4102 GN=HSP22 PE=2...	[more]

BLAST of CmaCh14G013470 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IQQ6	2.1e-112	100.00	26.5 kDa heat shock protein, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1GVM4	5.9e-107	95.71	26.5 kDa heat shock protein, mitochondrial isoform X1 OS=Cucurbita moschata OX=3...	[more]
A0A6J1GUB2	5.9e-107	95.71	26.5 kDa heat shock protein, mitochondrial isoform X2 OS=Cucurbita moschata OX=3...	[more]
A0A5A7TMA9	1.1e-84	77.73	26.5 kDa heat shock protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3BYA0	1.1e-84	77.73	26.5 kDa heat shock protein, mitochondrial OS=Cucumis melo OX=3656 GN=LOC1034948...	[more]

BLAST of CmaCh14G013470 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022979416.1	4.3e-112	100.00	26.5 kDa heat shock protein, mitochondrial [Cucurbita maxima]	[more]
XP_023526860.1	4.9e-108	96.67	LOW QUALITY PROTEIN: 26.5 kDa heat shock protein, mitochondrial [Cucurbita pepo ...	[more]
KAG6581923.1	1.4e-107	96.19	26.5 kDa heat shock protein, mitochondrial, partial [Cucurbita argyrosperma subs...	[more]
XP_022955630.1	1.2e-106	95.71	26.5 kDa heat shock protein, mitochondrial isoform X1 [Cucurbita moschata]	[more]
XP_022955631.1	1.2e-106	95.71	26.5 kDa heat shock protein, mitochondrial isoform X2 [Cucurbita moschata]	[more]

BLAST of CmaCh14G013470 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52560.1	1.5e-33	40.60	HSP20-like chaperones superfamily protein	[more]
AT1G52560.2	4.2e-33	40.97	HSP20-like chaperones superfamily protein	[more]
AT4G27670.1	8.6e-18	44.23	heat shock protein 21	[more]
AT4G25200.1	7.0e-12	40.54	mitochondrion-localized small heat shock protein 23.6	[more]
AT5G51440.1	1.2e-11	43.88	HSP20-like chaperones superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 71..209 e-value: 1.2E-29 score: 105.0
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 101..208
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 111..209 e-value: 7.5E-25 score: 87.0
IPR002068	Alpha crystallin/Hsp20 domain	PROSITE	PS01031	SHSP	coord: 95..210 score: 23.937309
IPR031107	Small heat shock protein HSP20	PANTHER	PTHR11527	HEAT-SHOCK PROTEIN 20 FAMILY MEMBER	coord: 49..209
None	No IPR available	PANTHER	PTHR11527:SF296	26.5 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL	coord: 49..209
None	No IPR available	CDD	cd06464	ACD_sHsps-like	coord: 111..196 e-value: 1.67067E-23 score: 87.6096

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh14G013470.1	CmaCh14G013470.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051259	protein complex oligomerization
biological_process	GO:0006457	protein folding
biological_process	GO:0009408	response to heat
biological_process	GO:0042542	response to hydrogen peroxide
biological_process	GO:0009651	response to salt stress
molecular_function	GO:0043621	protein self-association
molecular_function	GO:0051082	unfolded protein binding