CmaCh14G012300 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh14G012300
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	D-3-phosphoglycerate dehydrogenase
Location	Cma_Chr14: 9762922 .. 9765446 (+)
RNA-Seq Expression	CmaCh14G012300
Synteny	CmaCh14G012300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTTGGTAAATTATGTTTTAATTATTCCCCAAAATTCCCAACCTTAAACCCATTCCCCACTATTTTAAGCTCACCCCTTCTCCTCCCTCCATCAACAGTCCTTCTCGTTTCTTTCCACTTAAAAAGCCCTTCAACGCCATCATTCACAGCACCAAATTCATCTCCCATGGCTTCCTCTTCTGCAAAATCCCTCTTTTCCTCAACCCATTTCGCCTCCTTCCACAGATCCTCCGATTCCAATTCCAATTCCAATTCCCCTGTTCTTCCCTTCCTTCACAGCTCCATTTCCACCTCCTCTGTTTCTCTCAGGAGTTCCAATTTGATTCCTGTTCGTAGCGTTTTGAAGACGGTGGAATCGTCGGAATTAGATGTTCGGAGCTCCGATCTCGTCGACCCCACTTTCCCAAATGACAGATCGACCATTCTGGTGGCCGAGAAACTTGGAGAGGCCGGTTTGAAGCTTCTCCGGAGCTTTGGGAACGTCGACTGCGCTTACGATCTCTCACCGGAAGAGCTTTGCGCTAAGATCTCTTCTTGCGACGCCTTGATTGTGCGTAGTGGCACCAAGGTGAACCGGCAGGTCTTTGAGGCTGCAAAAGGGCGTTTGAAGGTTGTTGGAAGAGCCGGTGTTGGTATTGATAATGTGGATCTGCAGGCCGCCACTGAGTTTGGTTGTCTTGTTGTTAATGCCCCTACTGCCAATACCATCGCCGCCGCCGAACATGGTATTGCCTTGCTTACTGCCATGGCCCGAAATGTTGCTCAGGCCGATGCCTCCATGAAAGCAGGTCGGTAATAAATGAATACCCTTTTGTTTCAATGTTTAGATCTGCTTATAATTGCTAACCCCTTCTGTTTTTGGGCGTTAAAAGTGTGTTTTGTTACCCCAAAATTGATGTCTTGTAATGGGTTTCCAGGAAAATGGGAACGGAGCAAGTATGTCGGAGTTTCACTGGTCGGAAAAACACTAGCGGTGATGGGATTCGGGAAAGTGGGATCGGAAATGGCGAGACGAGCAAAGGGGTTGGGGATGCAAGTGATAGCACACGACCCTTACACGCCGGTGGACAGAGCAAGAGCCATCGGCGTTGAATTGGTGTCGTTCAATCAAGCCATATCCACCGCCGATTTCATTTCGCTTCATATGCCGCTCACGCCGACAACCACAAAGCTTTTCAACGACGACACTTTTAGTCTGATGAAGAAAGGAGCTCGGTTGATTAACGTTGGCAGAGGAGGTCTTATTGATGAAGATGCCTTAATTAAAGCCCTCGACAGTGGAGTTGTTTCTCAGGTAATTCCGATTCTAAAAAATTGTTGTATTGAATGAAGTGCGCCATAATTTATTGAGCTCCGGTGGAATAGGCGGCGCTTGACGTCTTCGTGGAGGAGCCACCGGCGAAAGACAGCAAGCTGGTGCAGCATAAGAATGTTACCGTCACGCCTCATCTTGGAGCTAGCACCAAGGAGGCACAGGAAGGCGTTGCCATTGAAATAGCCGAAGCCGTGGTGGGTGCATTGAAAGGTGAACTTTCCGCCACCGCCGTCAACGCTCCGATGGTGGCCCCGGAGGTAACTAAGCTTAATTAATGCAAAAAATTTGGATTTGGGGGTGAAATTAAACGGGATTATAAACAGGTCCTGACGGAGTTGGCGCCGTACGTGATTCTGGCGGAGAAATTGGGGCGGCTGGCGGTACAGCTGGTCGGCGGAGGTAAGGGAATAAAATCAATTAAAGTAGTTTACCGATCAGGGCGAGCCCCAGACGATTTAGACACGAGGCTGTTACGAGCCATGATCACAAAAGGCATAATCGAGCCGATCTCCGACAGCCACATAAACCTGGTGAACGCGGATTTCACGGCGAAGCAGAAGGGTCTCCGTATAAGCGAGGAGCGAGTGCTGGTGGACGCGCCGCCGGAGTTCCCAGTGGAGTCAATTCAGATTCTGGTGTCCAACGTGGAGTCAAAGTTTGGGAGTGCGGTGTCGGAGACGGGTGAGGTGGCAATGGAAGGGAAGGTGAAATATGGAGTGCCGCATTTGACACGTGTAGGATGGTTTGACGTGGATGTGAGTTTGGAAGGGAATTTAATCCTGTGTCGACAGGTGGATCAGCCGGGAATGATCGGACGGGTGGGAAACATTCTGGGAGAGAATAATGTGAATGTGAATTTTATGAGCGTTGGGAGAACGGTAAGGAAGAAAAGGGCAATTATGGCAATTGGTGTGGATGAAGTGCCCACTAAGGATACTCTCGCCAAAATTGGACAAGTCGCTGCCGTTGAAGAGTTTGTGTTTCTAAACCTATAATTATGGGACTCACTTCCGACATGTCGGTTACTTCCTTTTTCTTTTTTTCTTTTTTTTTTTTTTTTTTTATAGTTTTTATGTCTTGTTGGCCAGATGCCCAAGCCCAAGCCCAAGCCCAAGCCCAGGCCCAAGCCCAGCCCTGTTTAATAATTATTTAAACTACTTTTTGTATTTTTGTGTATGTTGATGAAATAAAATGAAATGCAAGTGAGGAC

mRNA sequence

GGTTGGTAAATTATGTTTTAATTATTCCCCAAAATTCCCAACCTTAAACCCATTCCCCACTATTTTAAGCTCACCCCTTCTCCTCCCTCCATCAACAGTCCTTCTCGTTTCTTTCCACTTAAAAAGCCCTTCAACGCCATCATTCACAGCACCAAATTCATCTCCCATGGCTTCCTCTTCTGCAAAATCCCTCTTTTCCTCAACCCATTTCGCCTCCTTCCACAGATCCTCCGATTCCAATTCCAATTCCAATTCCCCTGTTCTTCCCTTCCTTCACAGCTCCATTTCCACCTCCTCTGTTTCTCTCAGGAGTTCCAATTTGATTCCTGTTCGTAGCGTTTTGAAGACGGTGGAATCGTCGGAATTAGATGTTCGGAGCTCCGATCTCGTCGACCCCACTTTCCCAAATGACAGATCGACCATTCTGGTGGCCGAGAAACTTGGAGAGGCCGGTTTGAAGCTTCTCCGGAGCTTTGGGAACGTCGACTGCGCTTACGATCTCTCACCGGAAGAGCTTTGCGCTAAGATCTCTTCTTGCGACGCCTTGATTGTGCGTAGTGGCACCAAGGTGAACCGGCAGGTCTTTGAGGCTGCAAAAGGGCGTTTGAAGGTTGTTGGAAGAGCCGGTGTTGGTATTGATAATGTGGATCTGCAGGCCGCCACTGAGTTTGGTTGTCTTGTTGTTAATGCCCCTACTGCCAATACCATCGCCGCCGCCGAACATGGTATTGCCTTGCTTACTGCCATGGCCCGAAATGTTGCTCAGGCCGATGCCTCCATGAAAGCAGGAAAATGGGAACGGAGCAAGTATGTCGGAGTTTCACTGGTCGGAAAAACACTAGCGGTGATGGGATTCGGGAAAGTGGGATCGGAAATGGCGAGACGAGCAAAGGGGTTGGGGATGCAAGTGATAGCACACGACCCTTACACGCCGGTGGACAGAGCAAGAGCCATCGGCGTTGAATTGGTGTCGTTCAATCAAGCCATATCCACCGCCGATTTCATTTCGCTTCATATGCCGCTCACGCCGACAACCACAAAGCTTTTCAACGACGACACTTTTAGTCTGATGAAGAAAGGAGCTCGGTTGATTAACGTTGGCAGAGGAGGTCTTATTGATGAAGATGCCTTAATTAAAGCCCTCGACAGTGGAGTTGTTTCTCAGGCGGCGCTTGACGTCTTCGTGGAGGAGCCACCGGCGAAAGACAGCAAGCTGGTGCAGCATAAGAATGTTACCGTCACGCCTCATCTTGGAGCTAGCACCAAGGAGGCACAGGAAGGCGTTGCCATTGAAATAGCCGAAGCCGTGGTGGGTGCATTGAAAGGTGAACTTTCCGCCACCGCCGTCAACGCTCCGATGGTGGCCCCGGAGGTCCTGACGGAGTTGGCGCCGTACGTGATTCTGGCGGAGAAATTGGGGCGGCTGGCGGTACAGCTGGTCGGCGGAGGTAAGGGAATAAAATCAATTAAAGTAGTTTACCGATCAGGGCGAGCCCCAGACGATTTAGACACGAGGCTGTTACGAGCCATGATCACAAAAGGCATAATCGAGCCGATCTCCGACAGCCACATAAACCTGGTGAACGCGGATTTCACGGCGAAGCAGAAGGGTCTCCGTATAAGCGAGGAGCGAGTGCTGGTGGACGCGCCGCCGGAGTTCCCAGTGGAGTCAATTCAGATTCTGGTGTCCAACGTGGAGTCAAAGTTTGGGAGTGCGGTGTCGGAGACGGGTGAGGTGGCAATGGAAGGGAAGGTGAAATATGGAGTGCCGCATTTGACACGTGTAGGATGGTTTGACGTGGATGTGAGTTTGGAAGGGAATTTAATCCTGTGTCGACAGGTGGATCAGCCGGGAATGATCGGACGGGTGGGAAACATTCTGGGAGAGAATAATGTGAATGTGAATTTTATGAGCGTTGGGAGAACGGTAAGGAAGAAAAGGGCAATTATGGCAATTGGTGTGGATGAAGTGCCCACTAAGGATACTCTCGCCAAAATTGGACAAGTCGCTGCCGTTGAAGAGTTTGTGTTTCTAAACCTATAATTATGGGACTCACTTCCGACATGTCGGTTACTTCCTTTTTCTTTTTTTCTTTTTTTTTTTTTTTTTTTATAGTTTTTATGTCTTGTTGGCCAGATGCCCAAGCCCAAGCCCAAGCCCAAGCCCAGGCCCAAGCCCAGCCCTGTTTAATAATTATTTAAACTACTTTTTGTATTTTTGTGTATGTTGATGAAATAAAATGAAATGCAAGTGAGGAC

Coding sequence (CDS)

ATGGCTTCCTCTTCTGCAAAATCCCTCTTTTCCTCAACCCATTTCGCCTCCTTCCACAGATCCTCCGATTCCAATTCCAATTCCAATTCCCCTGTTCTTCCCTTCCTTCACAGCTCCATTTCCACCTCCTCTGTTTCTCTCAGGAGTTCCAATTTGATTCCTGTTCGTAGCGTTTTGAAGACGGTGGAATCGTCGGAATTAGATGTTCGGAGCTCCGATCTCGTCGACCCCACTTTCCCAAATGACAGATCGACCATTCTGGTGGCCGAGAAACTTGGAGAGGCCGGTTTGAAGCTTCTCCGGAGCTTTGGGAACGTCGACTGCGCTTACGATCTCTCACCGGAAGAGCTTTGCGCTAAGATCTCTTCTTGCGACGCCTTGATTGTGCGTAGTGGCACCAAGGTGAACCGGCAGGTCTTTGAGGCTGCAAAAGGGCGTTTGAAGGTTGTTGGAAGAGCCGGTGTTGGTATTGATAATGTGGATCTGCAGGCCGCCACTGAGTTTGGTTGTCTTGTTGTTAATGCCCCTACTGCCAATACCATCGCCGCCGCCGAACATGGTATTGCCTTGCTTACTGCCATGGCCCGAAATGTTGCTCAGGCCGATGCCTCCATGAAAGCAGGAAAATGGGAACGGAGCAAGTATGTCGGAGTTTCACTGGTCGGAAAAACACTAGCGGTGATGGGATTCGGGAAAGTGGGATCGGAAATGGCGAGACGAGCAAAGGGGTTGGGGATGCAAGTGATAGCACACGACCCTTACACGCCGGTGGACAGAGCAAGAGCCATCGGCGTTGAATTGGTGTCGTTCAATCAAGCCATATCCACCGCCGATTTCATTTCGCTTCATATGCCGCTCACGCCGACAACCACAAAGCTTTTCAACGACGACACTTTTAGTCTGATGAAGAAAGGAGCTCGGTTGATTAACGTTGGCAGAGGAGGTCTTATTGATGAAGATGCCTTAATTAAAGCCCTCGACAGTGGAGTTGTTTCTCAGGCGGCGCTTGACGTCTTCGTGGAGGAGCCACCGGCGAAAGACAGCAAGCTGGTGCAGCATAAGAATGTTACCGTCACGCCTCATCTTGGAGCTAGCACCAAGGAGGCACAGGAAGGCGTTGCCATTGAAATAGCCGAAGCCGTGGTGGGTGCATTGAAAGGTGAACTTTCCGCCACCGCCGTCAACGCTCCGATGGTGGCCCCGGAGGTCCTGACGGAGTTGGCGCCGTACGTGATTCTGGCGGAGAAATTGGGGCGGCTGGCGGTACAGCTGGTCGGCGGAGGTAAGGGAATAAAATCAATTAAAGTAGTTTACCGATCAGGGCGAGCCCCAGACGATTTAGACACGAGGCTGTTACGAGCCATGATCACAAAAGGCATAATCGAGCCGATCTCCGACAGCCACATAAACCTGGTGAACGCGGATTTCACGGCGAAGCAGAAGGGTCTCCGTATAAGCGAGGAGCGAGTGCTGGTGGACGCGCCGCCGGAGTTCCCAGTGGAGTCAATTCAGATTCTGGTGTCCAACGTGGAGTCAAAGTTTGGGAGTGCGGTGTCGGAGACGGGTGAGGTGGCAATGGAAGGGAAGGTGAAATATGGAGTGCCGCATTTGACACGTGTAGGATGGTTTGACGTGGATGTGAGTTTGGAAGGGAATTTAATCCTGTGTCGACAGGTGGATCAGCCGGGAATGATCGGACGGGTGGGAAACATTCTGGGAGAGAATAATGTGAATGTGAATTTTATGAGCGTTGGGAGAACGGTAAGGAAGAAAAGGGCAATTATGGCAATTGGTGTGGATGAAGTGCCCACTAAGGATACTCTCGCCAAAATTGGACAAGTCGCTGCCGTTGAAGAGTTTGTGTTTCTAAACCTATAA

Protein sequence

MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLPFLHSSISTSSVSLRSSNLIPVRSVLKTVESSELDVRSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARRAKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFSLMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPPAKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPMVAPEVLTELAPYVILAEKLGRLAVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRVGWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVDEVPTKDTLAKIGQVAAVEEFVFLNL

Homology

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BLAST of CmaCh14G012300 vs. ExPASy Swiss-Prot
Match: O04130 (D-3-phosphoglycerate dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGDH2 PE=1 SV=2)

HSP 1 Score: 866.7 bits (2238), Expect = 1.6e-250
Identity = 470/634 (74.13%), Postives = 535/634 (84.38%), Query Frame = 0


Query: 1   MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLP-FLHSSISTSSVSLRSSNLIPVRSVL 60
           MA SS+ S   + +  S   S   + +S   VLP FLH   +T      S  L  + + L
Sbjct: 1   MAFSSSCSSVKAVN--SRWTSPSPSPSSRFAVLPAFLHRRYAT------SVKLTAISAAL 60

Query: 61  KTVESSEL--DVR------SSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYD 120
           KTVE + L  D R       SD  +PT P  R  ILV EKLGEAG+ LLR FG+VDC+YD
Sbjct: 61  KTVEQTTLTEDNRFSTVGSDSDEYNPTLPKPR--ILVTEKLGEAGVNLLREFGDVDCSYD 120

Query: 121 LSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCL 180
           LSPE+L  K++  DALIVRSGTKV R+VFEAAKGRLKVVGRAGVGIDNVDLQAATE GCL
Sbjct: 121 LSPEDLKKKVAESDALIVRSGTKVTREVFEAAKGRLKVVGRAGVGIDNVDLQAATEHGCL 180

Query: 181 VVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFG 240
           VVNAPTANT+AAAEHGIALL +MARNVAQADAS+KAGKWERSKYVGVSLVGKTLAVMGFG
Sbjct: 181 VVNAPTANTVAAAEHGIALLASMARNVAQADASIKAGKWERSKYVGVSLVGKTLAVMGFG 240

Query: 241 KVGSEMARRAKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTT 300
           KVG+E+ARRAKGLGM VI+HDPY P DRARA+GV+LVSF+QAISTADF+SLHMPLTP T 
Sbjct: 241 KVGTEVARRAKGLGMTVISHDPYAPADRARALGVDLVSFDQAISTADFVSLHMPLTPATK 300

Query: 301 KLFNDDTFSLMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPPAKDSKLV 360
           K+FND+TFS MKKG RLINV RGG+IDEDAL++ALD+G+V+QAALDVF EEPP+KDS+L+
Sbjct: 301 KVFNDETFSKMKKGVRLINVARGGVIDEDALVRALDAGIVAQAALDVFCEEPPSKDSRLI 360

Query: 361 QHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPMVAPEVLTELAPYV 420
           QH+NVTVTPHLGASTKEAQEGVAIEIAEAV GALKGELSATAVNAPMVAPEVL+EL PY+
Sbjct: 361 QHENVTVTPHLGASTKEAQEGVAIEIAEAVAGALKGELSATAVNAPMVAPEVLSELTPYI 420

Query: 421 ILAEKLGRLAVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINL 480
           +LAEKLGRLAVQL  GGKG++SI+VVYRS R  DDLDTRLLRAMITKGIIEPISDS++NL
Sbjct: 421 VLAEKLGRLAVQLASGGKGVQSIRVVYRSARDRDDLDTRLLRAMITKGIIEPISDSYVNL 480

Query: 481 VNADFTAKQKGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVK 540
           VNADF AKQKGLRISEER++VD+ PE+PV+SIQ+ + NVES F  AVS+ G++++EGKVK
Sbjct: 481 VNADFIAKQKGLRISEERMVVDSSPEYPVDSIQVQILNVESNFAGAVSDAGDISIEGKVK 540

Query: 541 YGVPHLTRVGWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKK 600
           YGVPHLT VG F VDVSLEGNLILCRQVDQPGMIG+VGNILGE NVNVNFMSVGRTV +K
Sbjct: 541 YGVPHLTCVGSFGVDVSLEGNLILCRQVDQPGMIGQVGNILGEQNVNVNFMSVGRTVLRK 600

Query: 601 RAIMAIGVDEVPTKDTLAKIGQVAAVEEFVFLNL 626
           +AIMAIGVDE P   TL +IG V+A+EEFVFL L
Sbjct: 601 QAIMAIGVDEEPDNKTLERIGGVSAIEEFVFLKL 624

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BLAST of CmaCh14G012300 vs. ExPASy Swiss-Prot
Match: O49485 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGDH1 PE=1 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 6.8e-241
Identity = 435/596 (72.99%), Postives = 514/596 (86.24%), Query Frame = 0


Query: 38  SSISTSSVSLRSSNLIPVRSVLKTVESSELDVRSSDLVD--------PTFPNDRSTILVA 97
           SSI+ ++ SLR+  L   RS L +  S     R  + +          T    + TILVA
Sbjct: 9   SSIAVATNSLRNVTL-SSRSPLPSAISVAFPSRGRNTLQRRLVLVSCSTGDGSKPTILVA 68

Query: 98  EKLGEAGLKLLRSFGNVDCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKV 157
           EKLG+AG+KLL    NVDC+Y+++PEEL  KIS CDALIVRSGTKV R+VFE++ GRLKV
Sbjct: 69  EKLGDAGIKLLEDVANVDCSYNMTPEELNIKISLCDALIVRSGTKVGREVFESSHGRLKV 128

Query: 158 VGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGK 217
           VGRAGVGIDNVDL AATEFGCLVVNAPTANTIAAAEHGIAL+ AMARNVAQADAS+KAG+
Sbjct: 129 VGRAGVGIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARNVAQADASVKAGE 188

Query: 218 WERSKYVGVSLVGKTLAVMGFGKVGSEMARRAKGLGMQVIAHDPYTPVDRARAIGVELVS 277
           W+R+KYVGVSLVGKTLAV+GFGKVG+E+ARRAKGLGM+VIAHDPY P DRA AIGV+LVS
Sbjct: 189 WKRNKYVGVSLVGKTLAVLGFGKVGTEVARRAKGLGMRVIAHDPYAPADRAHAIGVDLVS 248

Query: 278 FNQAISTADFISLHMPLTPTTTKLFNDDTFSLMKKGARLINVGRGGLIDEDALIKALDSG 337
           F++A++TADFISLHMPLTPTT+K+ ND+TF+ MKKG R++NV RGG+IDEDAL++ALD+G
Sbjct: 249 FDEALATADFISLHMPLTPTTSKILNDETFAKMKKGVRIVNVARGGVIDEDALVRALDAG 308

Query: 338 VVSQAALDVFVEEPPAKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALKGEL 397
           +V+QAALDVF +EPPAKDSKLVQH+ VTVTPHLGAST EAQEGVAIEIAEAVVGAL GEL
Sbjct: 309 IVAQAALDVFTKEPPAKDSKLVQHERVTVTPHLGASTMEAQEGVAIEIAEAVVGALNGEL 368

Query: 398 SATAVNAPMVAPEVLTELAPYVILAEKLGRLAVQLVGGGKGIKSIKVVYRSGRAPDDLDT 457
           +ATAVNAPMV+ EVLTEL PYV+LAEKLGRLAVQLV GG G+K+ K+ Y S RA DDLDT
Sbjct: 369 AATAVNAPMVSAEVLTELKPYVVLAEKLGRLAVQLVAGGSGVKNAKITYASARATDDLDT 428

Query: 458 RLLRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQILVSN 517
           RLLRAMITKGIIEPISD ++NLVNADFTAKQ+GLR+SEERVL+D  PE P+E+I + +SN
Sbjct: 429 RLLRAMITKGIIEPISDVYVNLVNADFTAKQRGLRLSEERVLLDGSPESPLETITVQLSN 488

Query: 518 VESKFGSAVSETGEVAMEGKVKYGVPHLTRVGWFDVDVSLEGNLILCRQVDQPGMIGRVG 577
           VESKF S++SE+GEV +EGKVK GVPHLT+VG F+VDV+LEG++ILCRQVDQPGMIG VG
Sbjct: 489 VESKFASSLSESGEVKVEGKVKDGVPHLTKVGSFEVDVTLEGSIILCRQVDQPGMIGTVG 548

Query: 578 NILGENNVNVNFMSVGRTVRKKRAIMAIGVDEVPTKDTLAKIGQVAAVEEFVFLNL 626
           +ILGE+NVNVNFMSVGR   +K+AIMAIGVD++P+K+TL KIG++ AVEEFVFL L
Sbjct: 549 SILGESNVNVNFMSVGRIAPRKQAIMAIGVDDIPSKETLKKIGEIPAVEEFVFLKL 603

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BLAST of CmaCh14G012300 vs. ExPASy Swiss-Prot
Match: Q9LT69 (D-3-phosphoglycerate dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGDH3 PE=1 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 1.6e-229
Identity = 410/587 (69.85%), Postives = 490/587 (83.48%), Query Frame = 0


Query: 44  SVSLRSSNLIPVRSVLKTVESSELDVRSSDLV-----DPTFPNDRSTILVAEKLGEAGLK 103
           + SL  S++    S L T  SS   +R    +       +    + TILV EKLG+AG+ 
Sbjct: 2   ATSLNLSSIFSSSSRLVTTPSSVFPIRQRRRIILVTSSSSGGGGKPTILVTEKLGQAGID 61

Query: 104 LLRSFGNVDCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGID 163
           LL+ + NVDC+YDLS EELC KIS CDALIVRSGTKV R VFE+++GRLKVVGRAGVGID
Sbjct: 62  LLKKYANVDCSYDLSLEELCTKISLCDALIVRSGTKVGRDVFESSRGRLKVVGRAGVGID 121

Query: 164 NVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGV 223
           NVDL AATE+GCLVVNAPTANT+AAAEHGIALLTAMARN+AQADAS+KAGKW R+KYVGV
Sbjct: 122 NVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQADASIKAGKWTRNKYVGV 181

Query: 224 SLVGKTLAVMGFGKVGSEMARRAKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTAD 283
           SLVGKTLAV+GFGKVGSE+ARRA+GLGM VI HDPY P DRARAIGVELVSF  AISTAD
Sbjct: 182 SLVGKTLAVLGFGKVGSEVARRARGLGMHVITHDPYAPADRARAIGVELVSFEVAISTAD 241

Query: 284 FISLHMPLTPTTTKLFNDDTFSLMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDV 343
           FISLH+PLT  T+K+ ND TF++MKKG R++NV RGG+IDE+AL++ALDSG+V+QAALDV
Sbjct: 242 FISLHLPLTAATSKMMNDVTFAMMKKGVRIVNVARGGVIDEEALLRALDSGIVAQAALDV 301

Query: 344 FVEEPPAKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPM 403
           F  EPP KD+KLV H++VT TPHLGAST EAQEGV+IE+AEAV+GAL+GEL+ATAVNAPM
Sbjct: 302 FTVEPPVKDNKLVLHESVTATPHLGASTMEAQEGVSIEVAEAVIGALRGELAATAVNAPM 361

Query: 404 VAPEVLTELAPYVILAEKLGRLAVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITK 463
           V  EVL EL PYV+LAEKLGRLAVQLV GG G+ ++KV Y S RAPDDLDTRLLRAM+ K
Sbjct: 362 VPLEVLRELKPYVVLAEKLGRLAVQLVTGGSGVNAVKVTYASSRAPDDLDTRLLRAMVIK 421

Query: 464 GIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAV 523
           GIIEPIS   INLVN+D+ AKQ+G++ISEER+++D  PE P+E I + ++NVES+F SA+
Sbjct: 422 GIIEPISSVFINLVNSDYIAKQRGVKISEERMVLDGSPENPIEYITVRIANVESRFASAL 481

Query: 524 SETGEVAMEGKVKYGVPHLTRVGWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVN 583
           SE+GE+ +EG+VK GVP LT+VG F VDVSLEG++ILCRQVDQPGMIG+V +ILG+ NVN
Sbjct: 482 SESGEIKVEGRVKQGVPSLTKVGLFGVDVSLEGSVILCRQVDQPGMIGKVASILGDENVN 541

Query: 584 VNFMSVGRTVRKKRAIMAIGVDEVPTKDTLAKIGQVAAVEEFVFLNL 626
           V+FMSVGR    K+A+MAIGVDE P+K+TL KIG + A+EEFVFL L
Sbjct: 542 VSFMSVGRIAPGKQAVMAIGVDEQPSKETLKKIGDIPAIEEFVFLKL 588

[Close]

BLAST of CmaCh14G012300 vs. ExPASy Swiss-Prot
Match: Q58424 (D-3-phosphoglycerate dehydrogenase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=serA PE=3 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 1.3e-106
Identity = 221/546 (40.48%), Postives = 327/546 (59.89%), Query Frame = 0


Query: 86  ILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKG 145
           ILV + L E  +K+L   G V+ A  L+ EEL  KI   D L+VRSGTKV R V E A+ 
Sbjct: 4   ILVTDPLHEDAIKILEEVGEVEVATGLTKEELLEKIKDADVLVVRSGTKVTRDVIEKAE- 63

Query: 146 RLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASM 205
           +LKV+GRAGVG+DN+D++AATE G +VVNAP A++I+ AE  + L+ A ARN+ QA AS+
Sbjct: 64  KLKVIGRAGVGVDNIDVEAATEKGIIVVNAPDASSISVAELTMGLMLAAARNIPQATASL 123

Query: 206 KAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARRAKGLGMQVIAHDPYTPVDRARAIGV 265
           K G+W+R ++ G+ L GKTL V+G G++G ++ +RAK  GM +I +DPY P + A ++GV
Sbjct: 124 KRGEWDRKRFKGIELYGKTLGVIGLGRIGQQVVKRAKAFGMNIIGYDPYIPKEVAESMGV 183

Query: 266 ELV-SFNQAISTADFISLHMPLTPTTTKLFNDDTFSLMKKGARLINVGRGGLIDEDALIK 325
           ELV   N+    ADFI+LH+PLTP T  +   +  +LMKK A ++N  RGGLIDE AL +
Sbjct: 184 ELVDDINELCKRADFITLHVPLTPKTRHIIGREQIALMKKNAIIVNCARGGLIDEKALYE 243

Query: 326 ALDSGVVSQAALDVFVEEPPAKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGA 385
           AL  G +  AALDVF EEPP KD+ L+   NV  TPH GAST+EAQ+     +AE +   
Sbjct: 244 ALKEGKIRAAALDVFEEEPP-KDNPLLTLDNVIGTPHQGASTEEAQKAAGTIVAEQIKKV 303

Query: 386 LKGELSATAVNAPMVAPEVLTELAPYVILAEKLGRLAVQLVGGGKGIKSIKVVYRSGRAP 445
           L+GEL+   VN P +  E L +L PY++LAE LG + +Q++ G   +  ++++Y    A 
Sbjct: 304 LRGELAENVVNMPNIPQEKLGKLKPYMLLAEMLGNIVMQVLDG--SVNRVELIYSGELAK 363

Query: 446 DDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQ 505
           +  D  L++    KG++ PI  + INLVNA   AK + + + E                 
Sbjct: 364 EKTD--LIKRAFLKGLLSPILLAGINLVNAPIIAKNRNINVVES---------------- 423

Query: 506 ILVSNVESKFGSAVSETGE-----VAMEGKVKYGVPHLTRVGWFDVDVSLEGNLILCRQV 565
              S  E K+G+A+  T E      ++ G +    P +  V  ++V    EG L + + +
Sbjct: 424 ---STSEEKYGNAIKITAESDKKKFSIVGAIINNKPVILEVDGYEVSFIPEGVLAIIKHI 483

Query: 566 DQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVDEVPTKDTLAKIGQVAAVEE 625
           D+PG IGRV   LG+  +N+  M VGR      ++M + +D    ++ + KI ++  +++
Sbjct: 484 DRPGTIGRVCITLGDYGINIASMQVGRKEPGGESVMLLNLDHTVPEEVIEKIKEIPNIKD 524

[Close]

BLAST of CmaCh14G012300 vs. ExPASy Swiss-Prot
Match: O29445 (D-3-phosphoglycerate dehydrogenase OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=serA PE=3 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 4.4e-99
Identity = 215/544 (39.52%), Postives = 330/544 (60.66%), Query Frame = 0


Query: 86  ILVAEKLGEAGLKLLRSFG-NVDCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAK 145
           +LVAE + E  +  +R  G  V+    +S EEL  ++   +A++VRS TKV+ +V +AAK
Sbjct: 3   VLVAEPISEEAIDYMRKNGLEVEVKTGMSREELIREVPKYEAIVVRSQTKVDAEVIQAAK 62

Query: 146 GRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADAS 205
             LK++GRAGVG+DN+D+ AAT+ G +VVNAP  NTI+ AEH IAL+ A AR + QAD S
Sbjct: 63  -NLKIIGRAGVGVDNIDINAATQRGIVVVNAPGGNTISTAEHAIALMLAAARKIPQADRS 122

Query: 206 MKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARRAKGLGMQVIAHDPYTPVDRARAIG 265
           +K GKWER K++G+ L GKT  V+G G+VG E+A+R K L M V+A+DP+   +RA  IG
Sbjct: 123 VKEGKWERKKFMGIELRGKTAGVIGLGRVGFEVAKRCKALEMNVLAYDPFVSKERAEQIG 182

Query: 266 VELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFSLMKKGARLINVGRGGLIDEDALIK 325
           V+LV F+  ++++D I++H+P T  T  L     F  MK G  ++N  RGG++DE AL +
Sbjct: 183 VKLVDFDTLLASSDVITVHVPRTKETIGLIGKGQFEKMKDGVIVVNAARGGIVDEAALYE 242

Query: 326 ALDSGVVSQAALDVFVEEPPAKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGA 385
           A+ +G V+ AALDV+ +EPP+ D+ L++  NV  TPH+ AST+EAQ  V + IAE +V  
Sbjct: 243 AIKAGKVAAAALDVYEKEPPSPDNPLLKLDNVVTTPHIAASTREAQLNVGMIIAEDIVNM 302

Query: 386 LKGELSATAVNAPMVAPEVLTELAPYVILAEKLGRLAVQLVGGGKGIKSIKVVYRSGRAP 445
            KG     AVN P + P     + P++ LAEK+G++A   +GG   I+ +KV   SG+  
Sbjct: 303 AKGLPVRNAVNLPSIEPSDFEFMMPFLTLAEKMGKIASVRLGG--AIRKVKVTC-SGKLA 362

Query: 446 DDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQ 505
              +T  +   + KG+ EPI  + INLV+A   A ++G+ I E +          VES++
Sbjct: 363 TK-NTEFVTRALLKGLFEPILSNEINLVSAKPVAVERGITIEESK----------VESVE 422

Query: 506 ILVSNVESKFGSAVSETG-EVAMEGKVKYGVPHLTRVGWFDVDVSLEGNLILCRQVDQPG 565
               + ES     V   G E+ + G        + ++  ++V+   +G+ I+    D+PG
Sbjct: 423 ----HYESLLEVWVESNGKEMYLAGTCFGNEYRILKIDVYNVNFVPKGHYIISLHEDKPG 482

Query: 566 MIGRVGNILGENNVNVNFMSVGRTVRKKRAI--MAIGVDEVPTKDTLAKIGQVAAVEEFV 625
           +IGRVG + G NN+N+  M VGR+  K   I  M + VD+ PT + L ++ ++  + +  
Sbjct: 483 VIGRVGTLFGRNNINIAGMIVGRSGDKPGGIQLMLLLVDDPPTPEVLEEMTKLDGIIDAT 527

[Close]

BLAST of CmaCh14G012300 vs. ExPASy TrEMBL
Match: A0A6J1IWT4 (D-3-phosphoglycerate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111479899 PE=3 SV=1)

HSP 1 Score: 1174.1 bits (3036), Expect = 0.0e+00
Identity = 625/625 (100.00%), Postives = 625/625 (100.00%), Query Frame = 0


Query: 1   MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLPFLHSSISTSSVSLRSSNLIPVRSVLK 60
           MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLPFLHSSISTSSVSLRSSNLIPVRSVLK
Sbjct: 1   MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLPFLHSSISTSSVSLRSSNLIPVRSVLK 60

Query: 61  TVESSELDVRSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAK 120
           TVESSELDVRSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAK
Sbjct: 61  TVESSELDVRSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAK 120

Query: 121 ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT 180
           ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT
Sbjct: 121 ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT 180

Query: 181 IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR 240
           IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR
Sbjct: 181 IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR 240

Query: 241 AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFS 300
           AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFS
Sbjct: 241 AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFS 300

Query: 301 LMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPPAKDSKLVQHKNVTVTP 360
           LMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPPAKDSKLVQHKNVTVTP
Sbjct: 301 LMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPPAKDSKLVQHKNVTVTP 360

Query: 361 HLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPMVAPEVLTELAPYVILAEKLGRL 420
           HLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPMVAPEVLTELAPYVILAEKLGRL
Sbjct: 361 HLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPMVAPEVLTELAPYVILAEKLGRL 420

Query: 421 AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQ 480
           AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQ
Sbjct: 421 AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQ 480

Query: 481 KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV 540
           KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV
Sbjct: 481 KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV 540

Query: 541 GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD 600
           GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD
Sbjct: 541 GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD 600

Query: 601 EVPTKDTLAKIGQVAAVEEFVFLNL 626
           EVPTKDTLAKIGQVAAVEEFVFLNL
Sbjct: 601 EVPTKDTLAKIGQVAAVEEFVFLNL 625

[Close]

BLAST of CmaCh14G012300 vs. ExPASy TrEMBL
Match: A0A6J1GYB1 (D-3-phosphoglycerate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111457950 PE=3 SV=1)

HSP 1 Score: 1157.5 bits (2993), Expect = 0.0e+00
Identity = 615/625 (98.40%), Postives = 619/625 (99.04%), Query Frame = 0


Query: 1   MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLPFLHSSISTSSVSLRSSNLIPVRSVLK 60
           MASSSAKSLFSSTHFASFHRSSDSNSN+NSPVLPFL SSISTSSVSLR+SNLIPVRSVLK
Sbjct: 1   MASSSAKSLFSSTHFASFHRSSDSNSNTNSPVLPFLQSSISTSSVSLRNSNLIPVRSVLK 60

Query: 61  TVESSELDVRSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAK 120
           TVESSELD RSSDLVDPTFPNDRSTILVAEKLGEAGLKLLR FGNVDCAYDLSPEELCAK
Sbjct: 61  TVESSELDARSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRGFGNVDCAYDLSPEELCAK 120

Query: 121 ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT 180
           ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT
Sbjct: 121 ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT 180

Query: 181 IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR 240
           IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR
Sbjct: 181 IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR 240

Query: 241 AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFS 300
           AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTF 
Sbjct: 241 AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFG 300

Query: 301 LMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPPAKDSKLVQHKNVTVTP 360
           LMKKGARLINVGRGGLIDEDALIKALD+GVVSQAALDVFVEEPP +DSKLVQHKNVTVTP
Sbjct: 301 LMKKGARLINVGRGGLIDEDALIKALDNGVVSQAALDVFVEEPPPQDSKLVQHKNVTVTP 360

Query: 361 HLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPMVAPEVLTELAPYVILAEKLGRL 420
           HLGASTKEAQEGVAIEIAEAVVGAL GELSATAVNAPMVAPEVLTELAPYVILAEKLGRL
Sbjct: 361 HLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLTELAPYVILAEKLGRL 420

Query: 421 AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQ 480
           AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQ
Sbjct: 421 AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQ 480

Query: 481 KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV 540
           KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV
Sbjct: 481 KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV 540

Query: 541 GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD 600
           GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD
Sbjct: 541 GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD 600

Query: 601 EVPTKDTLAKIGQVAAVEEFVFLNL 626
           EVPTKDTLAKIGQVAAVEEFVFLNL
Sbjct: 601 EVPTKDTLAKIGQVAAVEEFVFLNL 625

[Close]

BLAST of CmaCh14G012300 vs. ExPASy TrEMBL
Match: A0A1S3CKG7 (D-3-phosphoglycerate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103501977 PE=3 SV=1)

HSP 1 Score: 1031.2 bits (2665), Expect = 1.8e-297
Identity = 552/636 (86.79%), Postives = 586/636 (92.14%), Query Frame = 0


Query: 1   MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLPF-----------LHSSISTSSVSLRS 60
           MASSS+KSLFSS HF SF+RS    S+S SP+L F           LHSSI++SS S   
Sbjct: 1   MASSSSKSLFSSPHFTSFNRS----SHSKSPLLSFSTSSVSLNPRPLHSSITSSSSSSNR 60

Query: 61  SNLIPVRSVLKTVESSELDVRSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCA 120
            + IP+RSVLKT+ESS    ++SDLVDPT PNDRSTILVAEKLGEAGL+LLRSFGNV CA
Sbjct: 61  CSFIPLRSVLKTLESSAFVSQNSDLVDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCA 120

Query: 121 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180
           YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG
Sbjct: 121 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180

Query: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMG 240
           CLVVNAPTANTIAAAEHGIALLTAMARNVAQADAS+KAGKWER+KYVGVSLVGKTLAVMG
Sbjct: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASIKAGKWERNKYVGVSLVGKTLAVMG 240

Query: 241 FGKVGSEMARRAKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPT 300
           FGKVGSE+ARRAKGLGMQVIAHDPY PVDRARAIGVELVSFNQAISTADFISLHMPLT  
Sbjct: 241 FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTSA 300

Query: 301 TTKLFNDDTFSLMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPPAKDSK 360
           T+K+FNDDTF LMKKGARLINV RGG+IDEDAL++ALD+G V+QAALDVFVEEPP KDSK
Sbjct: 301 TSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDTGAVAQAALDVFVEEPPPKDSK 360

Query: 361 LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPMVAPEVLTELAP 420
           LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGAL GELSATAVNAPMVAPEVL+ELAP
Sbjct: 361 LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAP 420

Query: 421 YVILAEKLGRLAVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHI 480
           YV+LAEKLGRLAVQLV GG GIKS+KVVY+SGRAPDDLDTRLLRAMITKGIIEPISDSHI
Sbjct: 421 YVVLAEKLGRLAVQLVAGGSGIKSVKVVYKSGRAPDDLDTRLLRAMITKGIIEPISDSHI 480

Query: 481 NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGK 540
           NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQ++VS VESKF SAV+E GEVA+EGK
Sbjct: 481 NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQVVVSEVESKFASAVTEKGEVAIEGK 540

Query: 541 VKYGVPHLTRVGWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVR 600
           VKYGVPHL RVG FDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRT+R
Sbjct: 541 VKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTMR 600

Query: 601 KKRAIMAIGVDEVPTKDTLAKIGQVAAVEEFVFLNL 626
           +KRAIMAIGVDE P KDTL +IGQV A+EEFVFLNL
Sbjct: 601 RKRAIMAIGVDEEPNKDTLRQIGQVPAIEEFVFLNL 632

[Close]

BLAST of CmaCh14G012300 vs. ExPASy TrEMBL
Match: A0A5D3CA37 (D-3-phosphoglycerate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold606G00710 PE=3 SV=1)

HSP 1 Score: 1028.1 bits (2657), Expect = 1.5e-296
Identity = 553/640 (86.41%), Postives = 588/640 (91.88%), Query Frame = 0


Query: 1   MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLPF-----------LHSSISTSSVSLRS 60
           MASSS+KS+FSS HF SF+RS    S+S SP+L F           LHSSI++SS S  S
Sbjct: 1   MASSSSKSIFSSPHFTSFNRS----SHSKSPLLSFSTSSVSLNPRPLHSSITSSSSSSSS 60

Query: 61  S----NLIPVRSVLKTVESSELDVRSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGN 120
           S    + IP+RSVLKT+ESS    ++SDLVDPT PNDRSTILVAEKLGEAGL+LLRSFGN
Sbjct: 61  SSNRCSFIPLRSVLKTLESSAFVSQNSDLVDPTSPNDRSTILVAEKLGEAGLELLRSFGN 120

Query: 121 VDCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAA 180
           V CAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAA
Sbjct: 121 VVCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAA 180

Query: 181 TEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTL 240
           TEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADAS+KAGKWER+KYVGVSLVGKTL
Sbjct: 181 TEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASIKAGKWERNKYVGVSLVGKTL 240

Query: 241 AVMGFGKVGSEMARRAKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMP 300
           AVMGFGKVGSE+ARRAKGLGMQVIAHDPY PVDRARAIGVELVSFNQAISTADFISLHMP
Sbjct: 241 AVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMP 300

Query: 301 LTPTTTKLFNDDTFSLMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPPA 360
           LT  T+K+FNDDTF LMKKGARLINV RGG+IDEDAL++ALD+G V+QAALDVFVEEPP 
Sbjct: 301 LTSATSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDTGAVAQAALDVFVEEPPP 360

Query: 361 KDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPMVAPEVLT 420
           KDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGAL GELSATAVNAPMVAPEVL+
Sbjct: 361 KDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLS 420

Query: 421 ELAPYVILAEKLGRLAVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPIS 480
           ELAPYV+LAEKLGRLAVQLV GG GIKS+KVVY+SGRAPDDLDTRLLRAMITKGIIEPIS
Sbjct: 421 ELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVVYKSGRAPDDLDTRLLRAMITKGIIEPIS 480

Query: 481 DSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVA 540
           DSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQ++VS VESKF SAV+E GEVA
Sbjct: 481 DSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQVVVSEVESKFASAVTEKGEVA 540

Query: 541 MEGKVKYGVPHLTRVGWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVG 600
           +EGKVKYGVPHL RVG FDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVG
Sbjct: 541 IEGKVKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVG 600

Query: 601 RTVRKKRAIMAIGVDEVPTKDTLAKIGQVAAVEEFVFLNL 626
           RT+R+KRAIMAIGVDE P KDTL +IGQV A+EEFVFLNL
Sbjct: 601 RTMRRKRAIMAIGVDEEPNKDTLRQIGQVPAIEEFVFLNL 636

[Close]

BLAST of CmaCh14G012300 vs. ExPASy TrEMBL
Match: A0A0A0LC35 (D-3-phosphoglycerate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_3G651740 PE=3 SV=1)

HSP 1 Score: 1026.2 bits (2652), Expect = 5.9e-296
Identity = 550/636 (86.48%), Postives = 582/636 (91.51%), Query Frame = 0


Query: 1   MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLPF-----------LHSSISTSSVSLRS 60
           MASSS+ SLFSS HF SF+RS     +S  P+L F           LHSS S+SS S   
Sbjct: 1   MASSSSNSLFSSPHFTSFNRS----FHSKPPLLSFSTSSVSLNPRPLHSSSSSSSSSSNR 60

Query: 61  SNLIPVRSVLKTVESSELDVRSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCA 120
            + IP+RSVLKT+ESS    ++SDL+DPT PNDRSTILVAEKLGEAGL+LLRSFGNV CA
Sbjct: 61  CSFIPLRSVLKTLESSAFVSQNSDLLDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCA 120

Query: 121 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180
           YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG
Sbjct: 121 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180

Query: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMG 240
           CLVVNAPTANTIAAAEHGIALLT+MARNVAQADASMKAGKWER+KYVGVSLVGKTLAVMG
Sbjct: 181 CLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMG 240

Query: 241 FGKVGSEMARRAKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPT 300
           FGKVGSE+ARRAKGLGMQVIAHDPY PVDRARAIGVELVSFNQAISTADFISLHMPLTPT
Sbjct: 241 FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPT 300

Query: 301 TTKLFNDDTFSLMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPPAKDSK 360
           T+K+FNDDTF LMKKGARLINV RGG+IDEDAL++ALDSG V+QAALDVFVEEPP KDSK
Sbjct: 301 TSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDVFVEEPPPKDSK 360

Query: 361 LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPMVAPEVLTELAP 420
           LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGAL GELSATAVNAPMV PEVL+ELAP
Sbjct: 361 LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAP 420

Query: 421 YVILAEKLGRLAVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHI 480
           YV+LAEKLGRLAVQLV GG GIKS+KVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHI
Sbjct: 421 YVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHI 480

Query: 481 NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGK 540
           NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQI+VS VESKF SAV+E GEV +EGK
Sbjct: 481 NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAVTEKGEVVIEGK 540

Query: 541 VKYGVPHLTRVGWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVR 600
           VKYGVPHL RVG FDVDVSLEGNLILCRQVDQPGMIG+VGNILGENNVNVNFMSVGRT+R
Sbjct: 541 VKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVNVNFMSVGRTLR 600

Query: 601 KKRAIMAIGVDEVPTKDTLAKIGQVAAVEEFVFLNL 626
           +KRAIMAIG+DE P KDTL KIGQV A+EEFVFLNL
Sbjct: 601 RKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 632

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BLAST of CmaCh14G012300 vs. NCBI nr
Match: XP_022980570.1 (D-3-phosphoglycerate dehydrogenase 2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1174.1 bits (3036), Expect = 0.0e+00
Identity = 625/625 (100.00%), Postives = 625/625 (100.00%), Query Frame = 0


Query: 1   MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLPFLHSSISTSSVSLRSSNLIPVRSVLK 60
           MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLPFLHSSISTSSVSLRSSNLIPVRSVLK
Sbjct: 1   MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLPFLHSSISTSSVSLRSSNLIPVRSVLK 60

Query: 61  TVESSELDVRSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAK 120
           TVESSELDVRSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAK
Sbjct: 61  TVESSELDVRSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAK 120

Query: 121 ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT 180
           ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT
Sbjct: 121 ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT 180

Query: 181 IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR 240
           IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR
Sbjct: 181 IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR 240

Query: 241 AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFS 300
           AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFS
Sbjct: 241 AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFS 300

Query: 301 LMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPPAKDSKLVQHKNVTVTP 360
           LMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPPAKDSKLVQHKNVTVTP
Sbjct: 301 LMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPPAKDSKLVQHKNVTVTP 360

Query: 361 HLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPMVAPEVLTELAPYVILAEKLGRL 420
           HLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPMVAPEVLTELAPYVILAEKLGRL
Sbjct: 361 HLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPMVAPEVLTELAPYVILAEKLGRL 420

Query: 421 AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQ 480
           AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQ
Sbjct: 421 AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQ 480

Query: 481 KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV 540
           KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV
Sbjct: 481 KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV 540

Query: 541 GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD 600
           GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD
Sbjct: 541 GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD 600

Query: 601 EVPTKDTLAKIGQVAAVEEFVFLNL 626
           EVPTKDTLAKIGQVAAVEEFVFLNL
Sbjct: 601 EVPTKDTLAKIGQVAAVEEFVFLNL 625

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BLAST of CmaCh14G012300 vs. NCBI nr
Match: XP_022956179.1 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1157.5 bits (2993), Expect = 0.0e+00
Identity = 615/625 (98.40%), Postives = 619/625 (99.04%), Query Frame = 0


Query: 1   MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLPFLHSSISTSSVSLRSSNLIPVRSVLK 60
           MASSSAKSLFSSTHFASFHRSSDSNSN+NSPVLPFL SSISTSSVSLR+SNLIPVRSVLK
Sbjct: 1   MASSSAKSLFSSTHFASFHRSSDSNSNTNSPVLPFLQSSISTSSVSLRNSNLIPVRSVLK 60

Query: 61  TVESSELDVRSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAK 120
           TVESSELD RSSDLVDPTFPNDRSTILVAEKLGEAGLKLLR FGNVDCAYDLSPEELCAK
Sbjct: 61  TVESSELDARSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRGFGNVDCAYDLSPEELCAK 120

Query: 121 ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT 180
           ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT
Sbjct: 121 ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT 180

Query: 181 IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR 240
           IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR
Sbjct: 181 IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR 240

Query: 241 AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFS 300
           AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTF 
Sbjct: 241 AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFG 300

Query: 301 LMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPPAKDSKLVQHKNVTVTP 360
           LMKKGARLINVGRGGLIDEDALIKALD+GVVSQAALDVFVEEPP +DSKLVQHKNVTVTP
Sbjct: 301 LMKKGARLINVGRGGLIDEDALIKALDNGVVSQAALDVFVEEPPPQDSKLVQHKNVTVTP 360

Query: 361 HLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPMVAPEVLTELAPYVILAEKLGRL 420
           HLGASTKEAQEGVAIEIAEAVVGAL GELSATAVNAPMVAPEVLTELAPYVILAEKLGRL
Sbjct: 361 HLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLTELAPYVILAEKLGRL 420

Query: 421 AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQ 480
           AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQ
Sbjct: 421 AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQ 480

Query: 481 KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV 540
           KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV
Sbjct: 481 KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV 540

Query: 541 GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD 600
           GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD
Sbjct: 541 GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD 600

Query: 601 EVPTKDTLAKIGQVAAVEEFVFLNL 626
           EVPTKDTLAKIGQVAAVEEFVFLNL
Sbjct: 601 EVPTKDTLAKIGQVAAVEEFVFLNL 625

[Close]

BLAST of CmaCh14G012300 vs. NCBI nr
Match: XP_023528075.1 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1156.7 bits (2991), Expect = 0.0e+00
Identity = 616/625 (98.56%), Postives = 618/625 (98.88%), Query Frame = 0


Query: 1   MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLPFLHSSISTSSVSLRSSNLIPVRSVLK 60
           MASSSAKSL SSTHFASFHRS +SNSNSNSPVLPFL SSISTSSVSLRSSNLIPVRSVLK
Sbjct: 1   MASSSAKSLLSSTHFASFHRSPESNSNSNSPVLPFLQSSISTSSVSLRSSNLIPVRSVLK 60

Query: 61  TVESSELDVRSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAK 120
           TVESSELD RSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAK
Sbjct: 61  TVESSELDARSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAK 120

Query: 121 ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT 180
           ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT
Sbjct: 121 ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT 180

Query: 181 IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR 240
           IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR
Sbjct: 181 IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR 240

Query: 241 AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFS 300
           AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTF 
Sbjct: 241 AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFG 300

Query: 301 LMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPPAKDSKLVQHKNVTVTP 360
           LMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPP +DSKLVQHKNVTVTP
Sbjct: 301 LMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPPPQDSKLVQHKNVTVTP 360

Query: 361 HLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPMVAPEVLTELAPYVILAEKLGRL 420
           HLGASTKEAQEGVAIEIAEAVVGAL GELSATAVNAPMVAPEVLTELAPYVILAEKLGRL
Sbjct: 361 HLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLTELAPYVILAEKLGRL 420

Query: 421 AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQ 480
           AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQ
Sbjct: 421 AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQ 480

Query: 481 KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV 540
           KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV
Sbjct: 481 KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV 540

Query: 541 GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD 600
           GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD
Sbjct: 541 GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD 600

Query: 601 EVPTKDTLAKIGQVAAVEEFVFLNL 626
           EVPTKDTLAKIGQVAAVEEFVFLNL
Sbjct: 601 EVPTKDTLAKIGQVAAVEEFVFLNL 625

[Close]

BLAST of CmaCh14G012300 vs. NCBI nr
Match: KAG6581805.1 (D-3-phosphoglycerate dehydrogenase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 614/625 (98.24%), Postives = 617/625 (98.72%), Query Frame = 0


Query: 1   MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLPFLHSSISTSSVSLRSSNLIPVRSVLK 60
           MASSSAKSL SSTHFASFHRSSD NSNSNSPVLPFL SSISTSSVSLRSSNLIP+RSVLK
Sbjct: 1   MASSSAKSLLSSTHFASFHRSSDFNSNSNSPVLPFLQSSISTSSVSLRSSNLIPIRSVLK 60

Query: 61  TVESSELDVRSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAK 120
           TVESSELD RSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAK
Sbjct: 61  TVESSELDARSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAK 120

Query: 121 ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT 180
           ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT
Sbjct: 121 ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT 180

Query: 181 IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR 240
           IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR
Sbjct: 181 IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR 240

Query: 241 AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFS 300
           AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTF 
Sbjct: 241 AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFG 300

Query: 301 LMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPPAKDSKLVQHKNVTVTP 360
           LMKKGARLINVGRGGLIDEDALIKALD+GVVSQAALDVFVEEPP +DSKLVQHKNVTVTP
Sbjct: 301 LMKKGARLINVGRGGLIDEDALIKALDNGVVSQAALDVFVEEPPPQDSKLVQHKNVTVTP 360

Query: 361 HLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPMVAPEVLTELAPYVILAEKLGRL 420
           HLGASTKEAQEGVAIEIAEAVVGAL GELSATAVNAPMVAPEVLTELAPYVILAEKLGRL
Sbjct: 361 HLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLTELAPYVILAEKLGRL 420

Query: 421 AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQ 480
           AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDS INLVNADFTAKQ
Sbjct: 421 AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSRINLVNADFTAKQ 480

Query: 481 KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV 540
           KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV
Sbjct: 481 KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV 540

Query: 541 GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD 600
           GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD
Sbjct: 541 GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD 600

Query: 601 EVPTKDTLAKIGQVAAVEEFVFLNL 626
           EVPTKDTLAKIGQVAAVEEFVFLNL
Sbjct: 601 EVPTKDTLAKIGQVAAVEEFVFLNL 625

[Close]

BLAST of CmaCh14G012300 vs. NCBI nr
Match: KAG7018256.1 (D-3-phosphoglycerate dehydrogenase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1153.3 bits (2982), Expect = 0.0e+00
Identity = 614/625 (98.24%), Postives = 617/625 (98.72%), Query Frame = 0


Query: 1   MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLPFLHSSISTSSVSLRSSNLIPVRSVLK 60
           MASSSAKSL SSTHFASFHRSSD NSNSNSPVLPFL SSISTSSVSLRSSNLIP+RSVLK
Sbjct: 14  MASSSAKSLLSSTHFASFHRSSDFNSNSNSPVLPFLQSSISTSSVSLRSSNLIPIRSVLK 73

Query: 61  TVESSELDVRSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAK 120
           TVESSELD RSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAK
Sbjct: 74  TVESSELDARSSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYDLSPEELCAK 133

Query: 121 ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT 180
           ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT
Sbjct: 134 ISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANT 193

Query: 181 IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR 240
           IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR
Sbjct: 194 IAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFGKVGSEMARR 253

Query: 241 AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFS 300
           AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTF 
Sbjct: 254 AKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFG 313

Query: 301 LMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPPAKDSKLVQHKNVTVTP 360
           LMKKGARLINVGRGGLIDEDALIKALD+GVVSQAALDVFVEEPP +DSKLVQHKNVTVTP
Sbjct: 314 LMKKGARLINVGRGGLIDEDALIKALDNGVVSQAALDVFVEEPPPQDSKLVQHKNVTVTP 373

Query: 361 HLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPMVAPEVLTELAPYVILAEKLGRL 420
           HLGASTKEAQEGVAIEIAEAVVGAL GELSATAVNAPMVAPEVLTELAPYVILAEKLGRL
Sbjct: 374 HLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLTELAPYVILAEKLGRL 433

Query: 421 AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQ 480
           AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDS INLVNADFTAKQ
Sbjct: 434 AVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSRINLVNADFTAKQ 493

Query: 481 KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV 540
           KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV
Sbjct: 494 KGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRV 553

Query: 541 GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD 600
           GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD
Sbjct: 554 GWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVD 613

Query: 601 EVPTKDTLAKIGQVAAVEEFVFLNL 626
           EVPTKDTLAKIGQVAAVEEFVFLNL
Sbjct: 614 EVPTKDTLAKIGQVAAVEEFVFLNL 638

[Close]

BLAST of CmaCh14G012300 vs. TAIR 10
Match: AT1G17745.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 866.7 bits (2238), Expect = 1.1e-251
Identity = 470/634 (74.13%), Postives = 535/634 (84.38%), Query Frame = 0


Query: 1   MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLP-FLHSSISTSSVSLRSSNLIPVRSVL 60
           MA SS+ S   + +  S   S   + +S   VLP FLH   +T      S  L  + + L
Sbjct: 1   MAFSSSCSSVKAVN--SRWTSPSPSPSSRFAVLPAFLHRRYAT------SVKLTAISAAL 60

Query: 61  KTVESSEL--DVR------SSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYD 120
           KTVE + L  D R       SD  +PT P  R  ILV EKLGEAG+ LLR FG+VDC+YD
Sbjct: 61  KTVEQTTLTEDNRFSTVGSDSDEYNPTLPKPR--ILVTEKLGEAGVNLLREFGDVDCSYD 120

Query: 121 LSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCL 180
           LSPE+L  K++  DALIVRSGTKV R+VFEAAKGRLKVVGRAGVGIDNVDLQAATE GCL
Sbjct: 121 LSPEDLKKKVAESDALIVRSGTKVTREVFEAAKGRLKVVGRAGVGIDNVDLQAATEHGCL 180

Query: 181 VVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGVSLVGKTLAVMGFG 240
           VVNAPTANT+AAAEHGIALL +MARNVAQADAS+KAGKWERSKYVGVSLVGKTLAVMGFG
Sbjct: 181 VVNAPTANTVAAAEHGIALLASMARNVAQADASIKAGKWERSKYVGVSLVGKTLAVMGFG 240

Query: 241 KVGSEMARRAKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTT 300
           KVG+E+ARRAKGLGM VI+HDPY P DRARA+GV+LVSF+QAISTADF+SLHMPLTP T 
Sbjct: 241 KVGTEVARRAKGLGMTVISHDPYAPADRARALGVDLVSFDQAISTADFVSLHMPLTPATK 300

Query: 301 KLFNDDTFSLMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDVFVEEPPAKDSKLV 360
           K+FND+TFS MKKG RLINV RGG+IDEDAL++ALD+G+V+QAALDVF EEPP+KDS+L+
Sbjct: 301 KVFNDETFSKMKKGVRLINVARGGVIDEDALVRALDAGIVAQAALDVFCEEPPSKDSRLI 360

Query: 361 QHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPMVAPEVLTELAPYV 420
           QH+NVTVTPHLGASTKEAQEGVAIEIAEAV GALKGELSATAVNAPMVAPEVL+EL PY+
Sbjct: 361 QHENVTVTPHLGASTKEAQEGVAIEIAEAVAGALKGELSATAVNAPMVAPEVLSELTPYI 420

Query: 421 ILAEKLGRLAVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINL 480
           +LAEKLGRLAVQL  GGKG++SI+VVYRS R  DDLDTRLLRAMITKGIIEPISDS++NL
Sbjct: 421 VLAEKLGRLAVQLASGGKGVQSIRVVYRSARDRDDLDTRLLRAMITKGIIEPISDSYVNL 480

Query: 481 VNADFTAKQKGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAVSETGEVAMEGKVK 540
           VNADF AKQKGLRISEER++VD+ PE+PV+SIQ+ + NVES F  AVS+ G++++EGKVK
Sbjct: 481 VNADFIAKQKGLRISEERMVVDSSPEYPVDSIQVQILNVESNFAGAVSDAGDISIEGKVK 540

Query: 541 YGVPHLTRVGWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTVRKK 600
           YGVPHLT VG F VDVSLEGNLILCRQVDQPGMIG+VGNILGE NVNVNFMSVGRTV +K
Sbjct: 541 YGVPHLTCVGSFGVDVSLEGNLILCRQVDQPGMIGQVGNILGEQNVNVNFMSVGRTVLRK 600

Query: 601 RAIMAIGVDEVPTKDTLAKIGQVAAVEEFVFLNL 626
           +AIMAIGVDE P   TL +IG V+A+EEFVFL L
Sbjct: 601 QAIMAIGVDEEPDNKTLERIGGVSAIEEFVFLKL 624

[Close]

BLAST of CmaCh14G012300 vs. TAIR 10
Match: AT1G17745.2 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 845.1 bits (2182), Expect = 3.6e-245
Identity = 468/661 (70.80%), Postives = 535/661 (80.94%), Query Frame = 0


Query: 1   MASSSAKSLFSSTHFASFHRSSDSNSNSNSPVLP-FLHSSISTSSVSLRSSNLIPVRSVL 60
           MA SS+ S   + +  S   S   + +S   VLP FLH   +T      S  L  + + L
Sbjct: 1   MAFSSSCSSVKAVN--SRWTSPSPSPSSRFAVLPAFLHRRYAT------SVKLTAISAAL 60

Query: 61  KTVESSEL--DVR------SSDLVDPTFPNDRSTILVAEKLGEAGLKLLRSFGNVDCAYD 120
           KTVE + L  D R       SD  +PT P  R  ILV EKLGEAG+ LLR FG+VDC+YD
Sbjct: 61  KTVEQTTLTEDNRFSTVGSDSDEYNPTLPKPR--ILVTEKLGEAGVNLLREFGDVDCSYD 120

Query: 121 LSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCL 180
           LSPE+L  K++  DALIVRSGTKV R+VFEAAKGRLKVVGRAGVGIDNVDLQAATE GCL
Sbjct: 121 LSPEDLKKKVAESDALIVRSGTKVTREVFEAAKGRLKVVGRAGVGIDNVDLQAATEHGCL 180

Query: 181 VVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAG-----------KW---------- 240
           VVNAPTANT+AAAEHGIALL +MARNVAQADAS+KAG           +W          
Sbjct: 181 VVNAPTANTVAAAEHGIALLASMARNVAQADASIKAGTLNYLFLVLLLRWNCRQSKHQYT 240

Query: 241 ------ERSKYVGVSLVGKTLAVMGFGKVGSEMARRAKGLGMQVIAHDPYTPVDRARAIG 300
                 +RSKYVGVSLVGKTLAVMGFGKVG+E+ARRAKGLGM VI+HDPY P DRARA+G
Sbjct: 241 IETETEKRSKYVGVSLVGKTLAVMGFGKVGTEVARRAKGLGMTVISHDPYAPADRARALG 300

Query: 301 VELVSFNQAISTADFISLHMPLTPTTTKLFNDDTFSLMKKGARLINVGRGGLIDEDALIK 360
           V+LVSF+QAISTADF+SLHMPLTP T K+FND+TFS MKKG RLINV RGG+IDEDAL++
Sbjct: 301 VDLVSFDQAISTADFVSLHMPLTPATKKVFNDETFSKMKKGVRLINVARGGVIDEDALVR 360

Query: 361 ALDSGVVSQAALDVFVEEPPAKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGA 420
           ALD+G+V+QAALDVF EEPP+KDS+L+QH+NVTVTPHLGASTKEAQEGVAIEIAEAV GA
Sbjct: 361 ALDAGIVAQAALDVFCEEPPSKDSRLIQHENVTVTPHLGASTKEAQEGVAIEIAEAVAGA 420

Query: 421 LKGELSATAVNAPMVAPEVLTELAPYVILAEKLGRLAVQLVGGGKGIKSIKVVYRSGRAP 480
           LKGELSATAVNAPMVAPEVL+EL PY++LAEKLGRLAVQL  GGKG++SI+VVYRS R  
Sbjct: 421 LKGELSATAVNAPMVAPEVLSELTPYIVLAEKLGRLAVQLASGGKGVQSIRVVYRSARDR 480

Query: 481 DDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQ 540
           DDLDTRLLRAMITKGIIEPISDS++NLVNADF AKQKGLRISEER++VD+ PE+PV+SIQ
Sbjct: 481 DDLDTRLLRAMITKGIIEPISDSYVNLVNADFIAKQKGLRISEERMVVDSSPEYPVDSIQ 540

Query: 541 ILVSNVESKFGSAVSETGEVAMEGKVKYGVPHLTRVGWFDVDVSLEGNLILCRQVDQPGM 600
           + + NVES F  AVS+ G++++EGKVKYGVPHLT VG F VDVSLEGNLILCRQVDQPGM
Sbjct: 541 VQILNVESNFAGAVSDAGDISIEGKVKYGVPHLTCVGSFGVDVSLEGNLILCRQVDQPGM 600

Query: 601 IGRVGNILGENNVNVNFMSVGRTVRKKRAIMAIGVDEVPTKDTLAKIGQVAAVEEFVFLN 626
           IG+VGNILGE NVNVNFMSVGRTV +K+AIMAIGVDE P   TL +IG V+A+EEFVFL 
Sbjct: 601 IGQVGNILGEQNVNVNFMSVGRTVLRKQAIMAIGVDEEPDNKTLERIGGVSAIEEFVFLK 651

[Close]

BLAST of CmaCh14G012300 vs. TAIR 10
Match: AT4G34200.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 834.7 bits (2155), Expect = 4.8e-242
Identity = 435/596 (72.99%), Postives = 514/596 (86.24%), Query Frame = 0


Query: 38  SSISTSSVSLRSSNLIPVRSVLKTVESSELDVRSSDLVD--------PTFPNDRSTILVA 97
           SSI+ ++ SLR+  L   RS L +  S     R  + +          T    + TILVA
Sbjct: 9   SSIAVATNSLRNVTL-SSRSPLPSAISVAFPSRGRNTLQRRLVLVSCSTGDGSKPTILVA 68

Query: 98  EKLGEAGLKLLRSFGNVDCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKV 157
           EKLG+AG+KLL    NVDC+Y+++PEEL  KIS CDALIVRSGTKV R+VFE++ GRLKV
Sbjct: 69  EKLGDAGIKLLEDVANVDCSYNMTPEELNIKISLCDALIVRSGTKVGREVFESSHGRLKV 128

Query: 158 VGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGK 217
           VGRAGVGIDNVDL AATEFGCLVVNAPTANTIAAAEHGIAL+ AMARNVAQADAS+KAG+
Sbjct: 129 VGRAGVGIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARNVAQADASVKAGE 188

Query: 218 WERSKYVGVSLVGKTLAVMGFGKVGSEMARRAKGLGMQVIAHDPYTPVDRARAIGVELVS 277
           W+R+KYVGVSLVGKTLAV+GFGKVG+E+ARRAKGLGM+VIAHDPY P DRA AIGV+LVS
Sbjct: 189 WKRNKYVGVSLVGKTLAVLGFGKVGTEVARRAKGLGMRVIAHDPYAPADRAHAIGVDLVS 248

Query: 278 FNQAISTADFISLHMPLTPTTTKLFNDDTFSLMKKGARLINVGRGGLIDEDALIKALDSG 337
           F++A++TADFISLHMPLTPTT+K+ ND+TF+ MKKG R++NV RGG+IDEDAL++ALD+G
Sbjct: 249 FDEALATADFISLHMPLTPTTSKILNDETFAKMKKGVRIVNVARGGVIDEDALVRALDAG 308

Query: 338 VVSQAALDVFVEEPPAKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALKGEL 397
           +V+QAALDVF +EPPAKDSKLVQH+ VTVTPHLGAST EAQEGVAIEIAEAVVGAL GEL
Sbjct: 309 IVAQAALDVFTKEPPAKDSKLVQHERVTVTPHLGASTMEAQEGVAIEIAEAVVGALNGEL 368

Query: 398 SATAVNAPMVAPEVLTELAPYVILAEKLGRLAVQLVGGGKGIKSIKVVYRSGRAPDDLDT 457
           +ATAVNAPMV+ EVLTEL PYV+LAEKLGRLAVQLV GG G+K+ K+ Y S RA DDLDT
Sbjct: 369 AATAVNAPMVSAEVLTELKPYVVLAEKLGRLAVQLVAGGSGVKNAKITYASARATDDLDT 428

Query: 458 RLLRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQILVSN 517
           RLLRAMITKGIIEPISD ++NLVNADFTAKQ+GLR+SEERVL+D  PE P+E+I + +SN
Sbjct: 429 RLLRAMITKGIIEPISDVYVNLVNADFTAKQRGLRLSEERVLLDGSPESPLETITVQLSN 488

Query: 518 VESKFGSAVSETGEVAMEGKVKYGVPHLTRVGWFDVDVSLEGNLILCRQVDQPGMIGRVG 577
           VESKF S++SE+GEV +EGKVK GVPHLT+VG F+VDV+LEG++ILCRQVDQPGMIG VG
Sbjct: 489 VESKFASSLSESGEVKVEGKVKDGVPHLTKVGSFEVDVTLEGSIILCRQVDQPGMIGTVG 548

Query: 578 NILGENNVNVNFMSVGRTVRKKRAIMAIGVDEVPTKDTLAKIGQVAAVEEFVFLNL 626
           +ILGE+NVNVNFMSVGR   +K+AIMAIGVD++P+K+TL KIG++ AVEEFVFL L
Sbjct: 549 SILGESNVNVNFMSVGRIAPRKQAIMAIGVDDIPSKETLKKIGEIPAVEEFVFLKL 603

[Close]

BLAST of CmaCh14G012300 vs. TAIR 10
Match: AT3G19480.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 797.0 bits (2057), Expect = 1.1e-230
Identity = 410/587 (69.85%), Postives = 490/587 (83.48%), Query Frame = 0


Query: 44  SVSLRSSNLIPVRSVLKTVESSELDVRSSDLV-----DPTFPNDRSTILVAEKLGEAGLK 103
           + SL  S++    S L T  SS   +R    +       +    + TILV EKLG+AG+ 
Sbjct: 2   ATSLNLSSIFSSSSRLVTTPSSVFPIRQRRRIILVTSSSSGGGGKPTILVTEKLGQAGID 61

Query: 104 LLRSFGNVDCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGID 163
           LL+ + NVDC+YDLS EELC KIS CDALIVRSGTKV R VFE+++GRLKVVGRAGVGID
Sbjct: 62  LLKKYANVDCSYDLSLEELCTKISLCDALIVRSGTKVGRDVFESSRGRLKVVGRAGVGID 121

Query: 164 NVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAGKWERSKYVGV 223
           NVDL AATE+GCLVVNAPTANT+AAAEHGIALLTAMARN+AQADAS+KAGKW R+KYVGV
Sbjct: 122 NVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQADASIKAGKWTRNKYVGV 181

Query: 224 SLVGKTLAVMGFGKVGSEMARRAKGLGMQVIAHDPYTPVDRARAIGVELVSFNQAISTAD 283
           SLVGKTLAV+GFGKVGSE+ARRA+GLGM VI HDPY P DRARAIGVELVSF  AISTAD
Sbjct: 182 SLVGKTLAVLGFGKVGSEVARRARGLGMHVITHDPYAPADRARAIGVELVSFEVAISTAD 241

Query: 284 FISLHMPLTPTTTKLFNDDTFSLMKKGARLINVGRGGLIDEDALIKALDSGVVSQAALDV 343
           FISLH+PLT  T+K+ ND TF++MKKG R++NV RGG+IDE+AL++ALDSG+V+QAALDV
Sbjct: 242 FISLHLPLTAATSKMMNDVTFAMMKKGVRIVNVARGGVIDEEALLRALDSGIVAQAALDV 301

Query: 344 FVEEPPAKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALKGELSATAVNAPM 403
           F  EPP KD+KLV H++VT TPHLGAST EAQEGV+IE+AEAV+GAL+GEL+ATAVNAPM
Sbjct: 302 FTVEPPVKDNKLVLHESVTATPHLGASTMEAQEGVSIEVAEAVIGALRGELAATAVNAPM 361

Query: 404 VAPEVLTELAPYVILAEKLGRLAVQLVGGGKGIKSIKVVYRSGRAPDDLDTRLLRAMITK 463
           V  EVL EL PYV+LAEKLGRLAVQLV GG G+ ++KV Y S RAPDDLDTRLLRAM+ K
Sbjct: 362 VPLEVLRELKPYVVLAEKLGRLAVQLVTGGSGVNAVKVTYASSRAPDDLDTRLLRAMVIK 421

Query: 464 GIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQILVSNVESKFGSAV 523
           GIIEPIS   INLVN+D+ AKQ+G++ISEER+++D  PE P+E I + ++NVES+F SA+
Sbjct: 422 GIIEPISSVFINLVNSDYIAKQRGVKISEERMVLDGSPENPIEYITVRIANVESRFASAL 481

Query: 524 SETGEVAMEGKVKYGVPHLTRVGWFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVN 583
           SE+GE+ +EG+VK GVP LT+VG F VDVSLEG++ILCRQVDQPGMIG+V +ILG+ NVN
Sbjct: 482 SESGEIKVEGRVKQGVPSLTKVGLFGVDVSLEGSVILCRQVDQPGMIGKVASILGDENVN 541

Query: 584 VNFMSVGRTVRKKRAIMAIGVDEVPTKDTLAKIGQVAAVEEFVFLNL 626
           V+FMSVGR    K+A+MAIGVDE P+K+TL KIG + A+EEFVFL L
Sbjct: 542 VSFMSVGRIAPGKQAVMAIGVDEQPSKETLKKIGDIPAIEEFVFLKL 588

[Close]

BLAST of CmaCh14G012300 vs. TAIR 10
Match: AT1G68010.1 (hydroxypyruvate reductase )

HSP 1 Score: 129.4 bits (324), Expect = 1.0e-29
Identity = 105/298 (35.23%), Postives = 153/298 (51.34%), Query Frame = 0


Query: 112 LSPEELCAKI-SSCDALIVRSGTKVNRQVFEA-AKGRLKVVGRAGVGIDNVDLQAATEFG 171
           LS E++   I   CD +I +        +F A +K   K      VG +NVD++AA ++G
Sbjct: 50  LSVEDIIDLIGDKCDGVIGQLTEDWGETLFSALSKAGGKAFSNMAVGYNNVDVEAANKYG 109

Query: 172 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASMKAG---KWERSKYVGVSLVGKTLA 231
             V N P   T   AE   +L  A AR + +AD  M+ G    W    +VG  L G+T+ 
Sbjct: 110 IAVGNTPGVLTETTAELAASLSLAAARRIVEADEFMRGGLYEGWLPHLFVGNLLKGQTVG 169

Query: 232 VMGFGKVGSEMAR-RAKGLGMQVIAHDPY--TPVDR--------ARAIGVELVSFNQAIS 291
           V+G G++GS  AR   +G  M +I  D Y  T +++         +A G + V++ +A S
Sbjct: 170 VIGAGRIGSAYARMMVEGFKMNLIYFDLYQSTRLEKFVTAYGQFLKANGEQPVTWKRASS 229

Query: 292 ------TADFISLHMPLTPTTTKLFNDDTFSLMKKGARLINVGRGGLIDEDALIKALDSG 351
                  AD ISLH  L  TT  L N +  ++MKK A L+N  RG +IDE AL++ L   
Sbjct: 230 MEEVLREADLISLHPVLDKTTYHLVNKERLAMMKKEAILVNCSRGPVIDEAALVEHLKEN 289

Query: 352 VVSQAALDVFVEEPPAKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALKG 388
            + +  LDVF EEP  K   L   KN  V PH+ +++K  +EG+A   A  V+G +KG
Sbjct: 290 PMFRVGLDVFEEEPFMKPG-LADTKNAIVVPHIASASKWTREGMATLAALNVLGRVKG 346

The following BLAST results are available for this feature:

BLAST of CmaCh14G012300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04130	1.6e-250	74.13	D-3-phosphoglycerate dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana OX=3...	[more]
O49485	6.8e-241	72.99	D-3-phosphoglycerate dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana OX=3...	[more]
Q9LT69	1.6e-229	69.85	D-3-phosphoglycerate dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana OX=3...	[more]
Q58424	1.3e-106	40.48	D-3-phosphoglycerate dehydrogenase OS=Methanocaldococcus jannaschii (strain ATCC...	[more]
O29445	4.4e-99	39.52	D-3-phosphoglycerate dehydrogenase OS=Archaeoglobus fulgidus (strain ATCC 49558 ...	[more]

BLAST of CmaCh14G012300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IWT4	0.0e+00	100.00	D-3-phosphoglycerate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111479899 P...	[more]
A0A6J1GYB1	0.0e+00	98.40	D-3-phosphoglycerate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111457950...	[more]
A0A1S3CKG7	1.8e-297	86.79	D-3-phosphoglycerate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103501977 PE=3 ...	[more]
A0A5D3CA37	1.5e-296	86.41	D-3-phosphoglycerate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A0A0LC35	5.9e-296	86.48	D-3-phosphoglycerate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_3G651740 PE...	[more]

BLAST of CmaCh14G012300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022980570.1	0.0e+00	100.00	D-3-phosphoglycerate dehydrogenase 2, chloroplastic-like [Cucurbita maxima]	[more]
XP_022956179.1	0.0e+00	98.40	D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucurbita moschata]	[more]
XP_023528075.1	0.0e+00	98.56	D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucurbita pepo subsp. ...	[more]
KAG6581805.1	0.0e+00	98.24	D-3-phosphoglycerate dehydrogenase 2, chloroplastic, partial [Cucurbita argyrosp...	[more]
KAG7018256.1	0.0e+00	98.24	D-3-phosphoglycerate dehydrogenase 2, chloroplastic, partial [Cucurbita argyrosp...	[more]

BLAST of CmaCh14G012300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17745.1	1.1e-251	74.13	D-3-phosphoglycerate dehydrogenase	[more]
AT1G17745.2	3.6e-245	70.80	D-3-phosphoglycerate dehydrogenase	[more]
AT4G34200.1	4.8e-242	72.99	D-3-phosphoglycerate dehydrogenase	[more]
AT3G19480.1	1.1e-230	69.85	D-3-phosphoglycerate dehydrogenase	[more]
AT1G68010.1	1.0e-29	35.23	hydroxypyruvate reductase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006139	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain	PFAM	PF00389	2-Hacid_dh	coord: 86..395 e-value: 2.2E-35 score: 122.1
None	No IPR available	GENE3D	3.40.50.720		coord: 179..361 e-value: 4.3E-111 score: 372.4
None	No IPR available	GENE3D	3.40.50.720		coord: 88..388 e-value: 4.3E-111 score: 372.4
None	No IPR available	GENE3D	3.30.70.260		coord: 552..625 e-value: 9.1E-21 score: 75.6
None	No IPR available	PANTHER	PTHR42938	FORMATE DEHYDROGENASE 1	coord: 37..620
None	No IPR available	PANTHER	PTHR42938:SF22	D-3-PHOSPHOGLYCERATE DEHYDROGENASE 2, CHLOROPLASTIC	coord: 37..620
None	No IPR available	CDD	cd04902	ACT_3PGDH-xct	coord: 553..625 e-value: 4.79055E-21 score: 85.2141
None	No IPR available	CDD	cd12173	PGDH_4	coord: 86..388 e-value: 8.21897E-163 score: 466.123
None	No IPR available	SUPERFAMILY	55021	ACT-like	coord: 551..623
None	No IPR available	SUPERFAMILY	52283	Formate/glycerate dehydrogenase catalytic domain-like	coord: 85..216
IPR002912	ACT domain	PFAM	PF01842	ACT	coord: 554..614 e-value: 8.1E-10 score: 38.3
IPR002912	ACT domain	PROSITE	PS51671	ACT	coord: 553..625 score: 10.462807
IPR006140	D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain	PFAM	PF02826	2-Hacid_dh_C	coord: 188..363 e-value: 1.4E-62 score: 210.2
IPR029009	Allosteric substrate binding domain superfamily	GENE3D	3.30.1330.90		coord: 400..551 e-value: 4.0E-27 score: 96.7
IPR029009	Allosteric substrate binding domain superfamily	SUPERFAMILY	143548	Serine metabolism enzymes domain	coord: 401..547
IPR006236	D-3-phosphoglycerate dehydrogenase	TIGRFAM	TIGR01327	TIGR01327	coord: 86..623 e-value: 1.2E-164 score: 547.0
IPR029752	D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved site 1	PROSITE	PS00065	D_2_HYDROXYACID_DH_1	coord: 225..252
IPR029753	D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved site	PROSITE	PS00670	D_2_HYDROXYACID_DH_2	coord: 273..295
IPR029753	D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved site	PROSITE	PS00671	D_2_HYDROXYACID_DH_3	coord: 302..318
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 182..364

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh14G012300.1	CmaCh14G012300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006564	L-serine biosynthetic process
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0051287	NAD binding
molecular_function	GO:0004617	phosphoglycerate dehydrogenase activity
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor