CmaCh14G011780 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh14G011780
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	delta(7)-sterol-C5(6)-desaturase-like
Location	Cma_Chr14: 9415014 .. 9417526 (+)
RNA-Seq Expression	CmaCh14G011780
Synteny	CmaCh14G011780

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCGGCGGACAGCCGTATCTGGAGCTCTTTGTGGCGGAGACTAGTTTCTACAACCGCATTGTTTTGGGAACCATTTTTCCTGCCTTTACTTTAGACCCATTTCCTCATATTGTTCAAACATGGCTTAGAAACTGCATTGGTGGATTATTAATTTACTTCTTCTCTGGCTTTCTTTGGTGCTTCTACATCTATTACTGGAAACGCAATGTCTATGTTCCTAAAGGTCTTTGCCTTTTTCTTTTTGCTCCTTTTTGTGTGTTTTTTTAACCCCTTTTGTGTCTATTTCTCAACTGCTCCATCAACCAGTTCTAATTTTGCTCATTTGGGTTCTATTAATTCACTTACTTCTTTGTTCACTCCCTTTTACAATCTGGATTTGTGTTTCTTTTTACTAATTTCACTCATTACTTCCAGATTCTTGGAGCTATAATCTTGAACTGCATGCTATATTTGATTCATTTTGGTTTAGATTGTAAATGATTACTAGTCCACATTGTTTTTGGATCATCTACATGTTCTTGTCAGATAGATTTCCCAAAAGGGTTGTTTTGATTTGTGCAAGCTGATGATTCTTCTTTCTGTGTTTGTTAGCTGAATTGTTATAAAAAGAACTGTAAAAAACAACATATATCCATAATAATTTAGAGCTAATGGATGATATATTATGTTTGGAGCTTCTTCTCGGTTAAACTAAGGATATTTTATTTGTTGTTCAAATGGAAAATATGATGTTTGTGATTTAGATATTGTTTTGGTTGTGACTAATTGCAAGAAACTAGTGGGGGATGTGTTGGTAGGTGGCTATGTTGAGCATTGAACTTGTTATTCATAGAGTATGTAAGAATCCAAGTAACTCATTTTTCGAGTTACGATATCGTTCGTGACGAACCGATGTTATATGTTATGAATGTGTATGGCTGAATGGTTGTTTTCTTGCAGATTCTATTCCATCTAATAAATTGATGCTCTTTCAAATGTTTGTAACGATCAAGGCCATGCCATTTTACTGCATCCTTCCGACGTTTGCTGAATACGTGGTTGAACATGATTGGACGAAATGCTATCCGAGGGTACTCGATGTCGGATGGCGTGCCTATGCCTTCCATATAGTTACTTATCTTGCCTTTGTAGAGTTTTGTATTTATTGGATGCATAGGGCGCTGCATGATATAAAGCCTCTTTATAAGTATCTCCATGCTAAACATCATATTTACAATAAGAAAATTACTCTCTCTCCCTTTGCTGGTAAGTTCTTAATCTACTTCTCTCAATTTTTGCATATTCATGTCAAATAGATTGCATGCCATAATATGTGTTTCTTGTTCGATTTCGTAAGATGTGTTCAACTTTGTATGAGATACCAGAATACGAGCGAACCAATCTCAACGAAACTATTACGAACCCTGGTGTTAAAACAGGGTGCTAGAACACTTTTATCTGATCCAAGCTAATTTGAGAAATACATTGGCATAGCATGAACACCTTAGGACTTACCTTGTCGCATGAACTCGACTTTTAGCGTGCTTTTGTGTATTATTTTGTCATCGATGTTTACGATGAAAACATGTAGTTCGTAAGCTGTTCTTCGTTTTGATAGAAACTTCAGGGATCTACTAAAGACTAAAGAAGTTCTATAGCTGTTTTTTATTAGAAGATGTTCTGATGAATGGTGTTTTTGAATGGGCAGGTTTGGCATTTCACCCTGTTGATGGGATATTGCAAGCAATACCTCATTTGTTTGCTCTATTTCTCATTCCAACTCATTTTAGAACACATATTGTGCTACTATTCTTCGAGGTGGTATGGACGACGAACATTCACGATTGTATCCACGCTCAAATATGGCCAGTTATGGGTGCTGGTTACCACACGATTCATCATACGAGATACCGCTACAATTATGGGCATTACTCCATATGGATGGATTGGATGTTCGGTACGCTTCTTGACCCGATTGATACAGAAGCCAGGGGGTCGTGATTGGTTGCTTCGACATCAACTGCTAACTCGCTATAGCTTTTGGTGATTCTTTGAGTTTAATGTAAATCAAGTTGGTTATTGATGAGGGCAGGTGTTGTGGTATGTTGCAGAATAAATTGGCATTCCAGAGGCAATTGGTGAAGCCATTCATACCATCAAATAAAGCATTCAATATCAGAGTTGGAATCCACAGGGATTATGTGCTGCTGCTGCTGCTATTGGTAGGCATTAGTTGCATCTTTGTGTATCATATTTAAATGAAAACTTTGGTTTGAAAAAATAGGAAATGACTATGTTTGGGGAATGGAATTCATGACTTCCCCTAATCGTGGAATGGAACCGCTCAAGCTAGATATTGTCCGCTTTGTTCCGTTAAGTATTGTTGTCAAACTCACGATTTTAAAGTTCGTCTACTAAGGAGAGACTTTCACACCCTTATAAGAAATACTTTGTTTCCTTCTTAAACGTGTCTATTAGGGAGAAAATTTATTTCCCTCTCGAATGCGTCTACTAGAGAGAGGTTTTCAGAC

mRNA sequence

ATGGGCGGCGGACAGCCGTATCTGGAGCTCTTTGTGGCGGAGACTAGTTTCTACAACCGCATTGTTTTGGGAACCATTTTTCCTGCCTTTACTTTAGACCCATTTCCTCATATTGTTCAAACATGGCTTAGAAACTGCATTGGTGGATTATTAATTTACTTCTTCTCTGGCTTTCTTTGGTGCTTCTACATCTATTACTGGAAACGCAATGTCTATGTTCCTAAAGATTCTATTCCATCTAATAAATTGATGCTCTTTCAAATGTTTGTAACGATCAAGGCCATGCCATTTTACTGCATCCTTCCGACGTTTGCTGAATACGTGGTTGAACATGATTGGACGAAATGCTATCCGAGGGTACTCGATGTCGGATGGCGTGCCTATGCCTTCCATATAGTTACTTATCTTGCCTTTGTAGAGTTTTGTATTTATTGGATGCATAGGGCGCTGCATGATATAAAGCCTCTTTATAAGTATCTCCATGCTAAACATCATATTTACAATAAGAAAATTACTCTCTCTCCCTTTGCTGGTTTGGCATTTCACCCTGTTGATGGGATATTGCAAGCAATACCTCATTTGTTTGCTCTATTTCTCATTCCAACTCATTTTAGAACACATATTGTGCTACTATTCTTCGAGGTGGTATGGACGACGAACATTCACGATTGTATCCACGCTCAAATATGGCCAGTTATGGGTGCTGGTTACCACACGATTCATCATACGAGATACCGCTACAATTATGGGCATTACTCCATATGGATGGATTGGATGTTCGGTACGCTTCTTGACCCGATTGATACAGAAGCCAGGGGGTCGTGATTGGTTGCTTCGACATCAACTGCTAACTCGCTATAGCTTTTGGTGATTCTTTGAGTTTAATGTAAATCAAGTTGGTTATTGATGAGGGCAGGTGTTGTGGTATGTTGCAGAATAAATTGGCATTCCAGAGGCAATTGGTGAAGCCATTCATACCATCAAATAAAGCATTCAATATCAGAGTTGGAATCCACAGGGATTATGTGCTGCTGCTGCTGCTATTGGTAGGCATTAGTTGCATCTTTGTGTATCATATTTAAATGAAAACTTTGGTTTGAAAAAATAGGAAATGACTATGTTTGGGGAATGGAATTCATGACTTCCCCTAATCGTGGAATGGAACCGCTCAAGCTAGATATTGTCCGCTTTGTTCCGTTAAGTATTGTTGTCAAACTCACGATTTTAAAGTTCGTCTACTAAGGAGAGACTTTCACACCCTTATAAGAAATACTTTGTTTCCTTCTTAAACGTGTCTATTAGGGAGAAAATTTATTTCCCTCTCGAATGCGTCTACTAGAGAGAGGTTTTCAGAC

Coding sequence (CDS)

ATGGGCGGCGGACAGCCGTATCTGGAGCTCTTTGTGGCGGAGACTAGTTTCTACAACCGCATTGTTTTGGGAACCATTTTTCCTGCCTTTACTTTAGACCCATTTCCTCATATTGTTCAAACATGGCTTAGAAACTGCATTGGTGGATTATTAATTTACTTCTTCTCTGGCTTTCTTTGGTGCTTCTACATCTATTACTGGAAACGCAATGTCTATGTTCCTAAAGATTCTATTCCATCTAATAAATTGATGCTCTTTCAAATGTTTGTAACGATCAAGGCCATGCCATTTTACTGCATCCTTCCGACGTTTGCTGAATACGTGGTTGAACATGATTGGACGAAATGCTATCCGAGGGTACTCGATGTCGGATGGCGTGCCTATGCCTTCCATATAGTTACTTATCTTGCCTTTGTAGAGTTTTGTATTTATTGGATGCATAGGGCGCTGCATGATATAAAGCCTCTTTATAAGTATCTCCATGCTAAACATCATATTTACAATAAGAAAATTACTCTCTCTCCCTTTGCTGGTTTGGCATTTCACCCTGTTGATGGGATATTGCAAGCAATACCTCATTTGTTTGCTCTATTTCTCATTCCAACTCATTTTAGAACACATATTGTGCTACTATTCTTCGAGGTGGTATGGACGACGAACATTCACGATTGTATCCACGCTCAAATATGGCCAGTTATGGGTGCTGGTTACCACACGATTCATCATACGAGATACCGCTACAATTATGGGCATTACTCCATATGGATGGATTGGATGTTCGGTACGCTTCTTGACCCGATTGATACAGAAGCCAGGGGGTCGTGA

Protein sequence

MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLWCFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRVLDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLAFHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTIHHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS

Homology

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BLAST of CmaCh14G011780 vs. ExPASy Swiss-Prot
Match: Q9ZT29 (Delta(7)-sterol-C5(6)-desaturase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 2.8e-114
Identity = 180/268 (67.16%), Postives = 218/268 (81.34%), Query Frame = 0


Query: 5   QPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLWCFYI 64
           + YL+ FV ETSFYNR+VLGT  P     P PH++Q WLRN IGG+L+YF SGFLWCFYI
Sbjct: 2   EDYLKQFVEETSFYNRLVLGTFMPESWWGPLPHMLQGWLRNYIGGVLLYFISGFLWCFYI 61

Query: 65  YYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRVLDVG 124
           Y+ KRNVY+PKD+IPSNK ML Q+ V +KAMP+YC LP+ +EY++E+ WTKC+ R+ DVG
Sbjct: 62  YHLKRNVYIPKDAIPSNKAMLLQISVAMKAMPWYCALPSLSEYMIENGWTKCFARISDVG 121

Query: 125 WRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLAFHPV 184
           W +Y  +   YL  VEF IYWMH  LHDIKPLYKYLHA HHIYNK+ TLSPFAGLAFHP+
Sbjct: 122 WLSYVIYAAIYLVIVEFGIYWMHMELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPL 181

Query: 185 DGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTIHHTR 244
           DGILQA+PH+ AL L+P HF THI L+F E +WT NIHDCIH +++PVMGAGYHTIHH  
Sbjct: 182 DGILQAVPHVVALLLVPMHFSTHIALIFLEALWTANIHDCIHGKVFPVMGAGYHTIHHRT 241

Query: 245 YRYNYGHYSIWMDWMFGTLLDPIDTEAR 273
           YR+NYGHY+IWMDWMFGTL DP++ +A+
Sbjct: 242 YRHNYGHYTIWMDWMFGTLRDPVEEDAK 269

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BLAST of CmaCh14G011780 vs. ExPASy Swiss-Prot
Match: Q39208 (Delta(7)-sterol-C5(6)-desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=STE1 PE=1 SV=2)

HSP 1 Score: 400.2 bits (1027), Expect = 1.9e-110
Identity = 180/267 (67.42%), Postives = 209/267 (78.28%), Query Frame = 0


Query: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60
           M     YL  FV ETSFYNRIVL  + PA   +P PH +QTWLRN + G L+YF SGFLW
Sbjct: 1   MAADNAYLMQFVDETSFYNRIVLSHLLPANLWEPLPHFLQTWLRNYLAGTLLYFISGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120
           CFYIYY K NVY+PKD+IP+ K M  QMFV +KAMP+Y +LPT +E ++E  WTKC+  +
Sbjct: 61  CFYIYYLKINVYLPKDAIPTIKAMRLQMFVAMKAMPWYTLLPTVSESMIERGWTKCFASI 120

Query: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
            + GW  Y  +I  YL FVEF IYWMHR LHDIKPLYKYLHA HHIYNK+ TLSPFAGLA
Sbjct: 121 GEFGWILYFVYIAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240
           FHPVDGILQA+PH+ ALF++P HF THI LLF E +WT NIHDCIH  IWPVMGAGYHTI
Sbjct: 181 FHPVDGILQAVPHVIALFIVPIHFTTHIGLLFMEAIWTANIHDCIHGNIWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPI 268
           HHT Y++NYGHY+IWMDWMFG+L DP+
Sbjct: 241 HHTTYKHNYGHYTIWMDWMFGSLRDPL 267

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BLAST of CmaCh14G011780 vs. ExPASy Swiss-Prot
Match: Q9M883 (Putative Delta(7)-sterol-C5(6)-desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=HDF7 PE=3 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 1.6e-101
Identity = 163/261 (62.45%), Postives = 202/261 (77.39%), Query Frame = 0


Query: 7   YLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLWCFYIYY 66
           Y +  V ETSFYNR+VL  + P    +P PH +QTWLRN + G ++YF SGFLWCFYIYY
Sbjct: 8   YNDQIVNETSFYNRMVLSHLLPVNLWEPLPHFLQTWLRNYLAGNILYFISGFLWCFYIYY 67

Query: 67  WKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRVLDVGWR 126
            K NVYVPK+SIP+ K ML Q++V +KAMP+Y +LP  +EY++EH WTKCY  +    W 
Sbjct: 68  LKLNVYVPKESIPTRKAMLLQIYVAMKAMPWYTLLPAVSEYMIEHGWTKCYSTLDHFNWF 127

Query: 127 AYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLAFHPVDG 186
               +I  YL  VEF IYW+H+ LHDIK LYK+LHA HH+YNK+ TLSPFAGLAFHP+DG
Sbjct: 128 LCFLYIALYLVLVEFMIYWVHKELHDIKFLYKHLHATHHMYNKQNTLSPFAGLAFHPLDG 187

Query: 187 ILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTIHHTRYR 246
           ILQAIPH+ ALF++P H  TH+ LLF E +WT +IHDCIH  IWP+MGAGYHTIHHT Y+
Sbjct: 188 ILQAIPHVIALFIVPIHLITHLSLLFLEGIWTASIHDCIHGNIWPIMGAGYHTIHHTTYK 247

Query: 247 YNYGHYSIWMDWMFGTLLDPI 268
           +NYGHY+IWMDWMFG+L+ P+
Sbjct: 248 HNYGHYTIWMDWMFGSLMVPL 268

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BLAST of CmaCh14G011780 vs. ExPASy Swiss-Prot
Match: Q9EQS5 (Lathosterol oxidase OS=Rattus norvegicus OX=10116 GN=Sc5d PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.6e-24
Identity = 79/234 (33.76%), Postives = 126/234 (53.85%), Query Frame = 0


Query: 47  IGGLLIYFFSGFLWCFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAE 106
           +G  ++YFF   L  +++Y    +  +       N++    MF T+K++P+  I PT + 
Sbjct: 40  LGAYILYFFCATLSYYFVY---DHSLMKHPQFLKNQVSREIMF-TVKSLPWISI-PTVSL 99

Query: 107 YVVE-HDWTKCYPRVLDV--GWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAK 166
           +++E   ++K Y  + D   GW      ++++L F +  IYW+HR LH  + LYK++H  
Sbjct: 100 FLLELRGYSKLYDDIGDFPNGWIHLIMSVISFLFFTDMLIYWIHRGLHH-RLLYKHIHKP 159

Query: 167 HHIYNKKITLSPFAGLAFHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHD 226
           HHI+  KI  +PFA  AFHPVDG LQ++P+    F+ P H   ++ L     VWT +IHD
Sbjct: 160 HHIW--KIP-TPFASHAFHPVDGFLQSLPYHIYPFVFPLHKVVYLGLYVLVNVWTISIHD 219

Query: 227 CIH--AQIWP--VMGAGYHTIHHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARG 274
                 QI+   + G+ +HT HH  + YNYG Y    D + G+   P   E +G
Sbjct: 220 GDFRVPQIFRPFINGSAHHTDHHMLFDYNYGQYFTLWDRIGGSFKHPSSFEGKG 264

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BLAST of CmaCh14G011780 vs. ExPASy Swiss-Prot
Match: O88822 (Lathosterol oxidase OS=Mus musculus OX=10090 GN=Sc5d PE=2 SV=2)

HSP 1 Score: 110.2 bits (274), Expect = 3.9e-23
Identity = 76/234 (32.48%), Postives = 125/234 (53.42%), Query Frame = 0


Query: 47  IGGLLIYFFSGFLWCFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAE 106
           +G  ++YFF   L  +++Y    +  +       N++   ++  T+K++P+  I PT + 
Sbjct: 40  LGAYILYFFCATLSYYFVY---DHSLMKHPQFLKNQVSR-EIVFTVKSLPWISI-PTVSL 99

Query: 107 YVVE-HDWTKCYPRVLDV--GWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAK 166
           +++E   ++K Y  + D   GW      +V++L F +  IYW+HR LH  + +YK +H  
Sbjct: 100 FLLELRGYSKLYDDIGDFPNGWIHLMVSVVSFLFFTDMLIYWIHRGLHH-RLVYKRIHKP 159

Query: 167 HHIYNKKITLSPFAGLAFHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHD 226
           HHI+  KI  +PFA  AFHPVDG LQ++P+    F+ P H   ++ L     VWT +IHD
Sbjct: 160 HHIW--KIP-TPFASHAFHPVDGFLQSLPYHIYPFVFPLHKVVYLGLYVLVNVWTISIHD 219

Query: 227 ---CIHAQIWP-VMGAGYHTIHHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARG 274
               +   + P + G+ +HT HH  + YNYG Y    D + G+   P   E +G
Sbjct: 220 GDFRVPQILRPFINGSAHHTDHHMFFDYNYGQYFTLWDRIGGSFKHPSSFEGKG 264

[Close]

BLAST of CmaCh14G011780 vs. ExPASy TrEMBL
Match: A0A6J1IX84 (delta(7)-sterol-C5(6)-desaturase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479986 PE=3 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 4.3e-166
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 0


Query: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60
           MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW
Sbjct: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120
           CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120

Query: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
           LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA
Sbjct: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240
           FHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI
Sbjct: 181 FHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS 275
           HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS 274

[Close]

BLAST of CmaCh14G011780 vs. ExPASy TrEMBL
Match: A0A6J1GYF7 (delta(7)-sterol-C5(6)-desaturase-like OS=Cucurbita moschata OX=3662 GN=LOC111457984 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 1.2e-165
Identity = 272/274 (99.27%), Postives = 274/274 (100.00%), Query Frame = 0


Query: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60
           MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW
Sbjct: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120
           CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120

Query: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
           LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA
Sbjct: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240
           FHPVDGILQAIPHLFALFLIPTHFRTH++LLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI
Sbjct: 181 FHPVDGILQAIPHLFALFLIPTHFRTHVMLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS 275
           HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS 274

[Close]

BLAST of CmaCh14G011780 vs. ExPASy TrEMBL
Match: A0A0A0L3B9 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G409800 PE=3 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 1.6e-152
Identity = 247/274 (90.15%), Postives = 260/274 (94.89%), Query Frame = 0


Query: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60
           MGGG PYLELFV ETSFYNRIVLGTIFP F+LDPFPHI+QTWLRNC+GG LIYFFSGFLW
Sbjct: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120
           CFYIYYWKRNVYVPKDSIPSNK ML Q+ VTIKAMP YC+LPTFAEYVVEHDWTKCYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120

Query: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
           LDVGW AYAFHI+TYL F+EFCIYWMHR LHDIKPLYKYLHAKHHIYNKKITLSPFAGLA
Sbjct: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240
           FHP+DGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWT NIHD IH+++WPVMGAGYHTI
Sbjct: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS 275
           HHTRYRYNYGHYSIWMDWMFGTLLDP+D EA+GS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKGS 274

[Close]

BLAST of CmaCh14G011780 vs. ExPASy TrEMBL
Match: A0A1S3BAC7 (delta(7)-sterol-C5(6)-desaturase-like OS=Cucumis melo OX=3656 GN=LOC103487532 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 2.5e-150
Identity = 244/273 (89.38%), Postives = 257/273 (94.14%), Query Frame = 0


Query: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60
           MGGG PYLELFV ETSFYNRIVLGTIFP F+L PFPHI+QTWLRNC+GG LIYFFSGFLW
Sbjct: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLYPFPHILQTWLRNCVGGFLIYFFSGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120
           CFYIYYWKRNVYVPKDSIPSNK ML Q+ VTIKAMP YC+LPTFAEYVVEHDWTKCYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120

Query: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
           LDVGW AYAFHI+TYL F+EFCIYWMHR LHDIKPLYKYLHAKHHIYNKK TLSPFAGLA
Sbjct: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKTTLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240
           FHP+DGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWT NIHD IH+++WPVMGAGYHTI
Sbjct: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARG 274
           HHTRYRYNYGHYSIWMDWMFGTLLDP+D EA+G
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKG 273

[Close]

BLAST of CmaCh14G011780 vs. ExPASy TrEMBL
Match: A0A6J1KDR9 (delta(7)-sterol-C5(6)-desaturase-like OS=Cucurbita maxima OX=3661 GN=LOC111492934 PE=3 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 5.8e-147
Identity = 237/270 (87.78%), Postives = 252/270 (93.33%), Query Frame = 0


Query: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60
           MGG  PYLELF+ ETS YNRIVLGTIFP F LDPFPHI+QTWLRNCIGG LIYFFSGFLW
Sbjct: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120
           CF+IYYWKRNVYVPKDSIPSNKLML Q+  TIKAMP YC+LPTF+EYVVEHDWT+CYPRV
Sbjct: 61  CFFIYYWKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120

Query: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
            DVGW AYAFH+ TYL FVEF IYWMHRALHD+KPLYKYLHAKHH+YNKK TLSPFAGLA
Sbjct: 121 FDVGWLAYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240
           FHPVDGILQAIPHLFALFLIP HFRTHI+LLFFEVVWTTNIHDCIHAQ+WPVMGAGYHT+
Sbjct: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTE 271
           HHTRYRYNYGHYSIWMDWMFGTLLDP+DT+
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQ 270

[Close]

BLAST of CmaCh14G011780 vs. NCBI nr
Match: XP_022980690.1 (delta(7)-sterol-C5(6)-desaturase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 593.6 bits (1529), Expect = 8.8e-166
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 0


Query: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60
           MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW
Sbjct: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120
           CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120

Query: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
           LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA
Sbjct: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240
           FHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI
Sbjct: 181 FHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS 275
           HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS 274

[Close]

BLAST of CmaCh14G011780 vs. NCBI nr
Match: XP_022956224.1 (delta(7)-sterol-C5(6)-desaturase-like [Cucurbita moschata] >KAG6581752.1 hypothetical protein SDJN03_21754, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026198.1 hypothetical protein SDJN02_12697, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 592.0 bits (1525), Expect = 2.6e-165
Identity = 272/274 (99.27%), Postives = 274/274 (100.00%), Query Frame = 0


Query: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60
           MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW
Sbjct: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120
           CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120

Query: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
           LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA
Sbjct: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240
           FHPVDGILQAIPHLFALFLIPTHFRTH++LLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI
Sbjct: 181 FHPVDGILQAIPHLFALFLIPTHFRTHVMLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS 275
           HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS 274

[Close]

BLAST of CmaCh14G011780 vs. NCBI nr
Match: XP_023527022.1 (delta(7)-sterol-C5(6)-desaturase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 592.0 bits (1525), Expect = 2.6e-165
Identity = 272/274 (99.27%), Postives = 274/274 (100.00%), Query Frame = 0


Query: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60
           MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW
Sbjct: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120
           CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTF+EYVVEHDWTKCYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFSEYVVEHDWTKCYPRV 120

Query: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
           LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA
Sbjct: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240
           FHPVDGILQAIPHLFALFLIPTHFRTH+VLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI
Sbjct: 181 FHPVDGILQAIPHLFALFLIPTHFRTHVVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS 275
           HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS 274

[Close]

BLAST of CmaCh14G011780 vs. NCBI nr
Match: XP_038894105.1 (delta(7)-sterol-C5(6)-desaturase-like [Benincasa hispida] >XP_038894106.1 delta(7)-sterol-C5(6)-desaturase-like [Benincasa hispida] >XP_038894107.1 delta(7)-sterol-C5(6)-desaturase-like [Benincasa hispida])

HSP 1 Score: 556.6 bits (1433), Expect = 1.2e-154
Identity = 249/274 (90.88%), Postives = 262/274 (95.62%), Query Frame = 0


Query: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60
           MGGG PYLELFV ET FYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGG LIYFFS FLW
Sbjct: 1   MGGGPPYLELFVEETDFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGFLIYFFSAFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120
           CFYIYYWKRNVY+PKDSIPSNK ML Q+FVTIKAMP YCILPTF+EYVVEHDWTKCYPRV
Sbjct: 61  CFYIYYWKRNVYIPKDSIPSNKSMLLQIFVTIKAMPLYCILPTFSEYVVEHDWTKCYPRV 120

Query: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
           LDVGW AYAFH++TYLAF+EF IYWMHR LHDIKPLYKYLHAKHHIYNKKITLSPFAGLA
Sbjct: 121 LDVGWPAYAFHVITYLAFIEFSIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240
           FHP+DGILQAIPHLFALFLIPTHFRTHIVLLFFEV+WTTNIHDCIHA++WPVMGAGYHTI
Sbjct: 181 FHPIDGILQAIPHLFALFLIPTHFRTHIVLLFFEVIWTTNIHDCIHARMWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS 275
           HHTRYRYNYGHYSIWMDWMFGTL+DP+DTE +GS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLIDPMDTEGKGS 274

[Close]

BLAST of CmaCh14G011780 vs. NCBI nr
Match: XP_004150515.1 (delta(7)-sterol-C5(6)-desaturase [Cucumis sativus] >KGN54626.1 hypothetical protein Csa_012304 [Cucumis sativus])

HSP 1 Score: 548.5 bits (1412), Expect = 3.2e-152
Identity = 247/274 (90.15%), Postives = 260/274 (94.89%), Query Frame = 0


Query: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60
           MGGG PYLELFV ETSFYNRIVLGTIFP F+LDPFPHI+QTWLRNC+GG LIYFFSGFLW
Sbjct: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120
           CFYIYYWKRNVYVPKDSIPSNK ML Q+ VTIKAMP YC+LPTFAEYVVEHDWTKCYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120

Query: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
           LDVGW AYAFHI+TYL F+EFCIYWMHR LHDIKPLYKYLHAKHHIYNKKITLSPFAGLA
Sbjct: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240
           FHP+DGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWT NIHD IH+++WPVMGAGYHTI
Sbjct: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS 275
           HHTRYRYNYGHYSIWMDWMFGTLLDP+D EA+GS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKGS 274

[Close]

BLAST of CmaCh14G011780 vs. TAIR 10
Match: AT3G02580.1 (sterol 1 )

HSP 1 Score: 400.2 bits (1027), Expect = 1.3e-111
Identity = 180/267 (67.42%), Postives = 209/267 (78.28%), Query Frame = 0


Query: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60
           M     YL  FV ETSFYNRIVL  + PA   +P PH +QTWLRN + G L+YF SGFLW
Sbjct: 1   MAADNAYLMQFVDETSFYNRIVLSHLLPANLWEPLPHFLQTWLRNYLAGTLLYFISGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120
           CFYIYY K NVY+PKD+IP+ K M  QMFV +KAMP+Y +LPT +E ++E  WTKC+  +
Sbjct: 61  CFYIYYLKINVYLPKDAIPTIKAMRLQMFVAMKAMPWYTLLPTVSESMIERGWTKCFASI 120

Query: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
            + GW  Y  +I  YL FVEF IYWMHR LHDIKPLYKYLHA HHIYNK+ TLSPFAGLA
Sbjct: 121 GEFGWILYFVYIAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240
           FHPVDGILQA+PH+ ALF++P HF THI LLF E +WT NIHDCIH  IWPVMGAGYHTI
Sbjct: 181 FHPVDGILQAVPHVIALFIVPIHFTTHIGLLFMEAIWTANIHDCIHGNIWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPI 268
           HHT Y++NYGHY+IWMDWMFG+L DP+
Sbjct: 241 HHTTYKHNYGHYTIWMDWMFGSLRDPL 267

[Close]

BLAST of CmaCh14G011780 vs. TAIR 10
Match: AT3G02590.1 (Fatty acid hydroxylase superfamily protein )

HSP 1 Score: 370.5 bits (950), Expect = 1.1e-102
Identity = 163/261 (62.45%), Postives = 202/261 (77.39%), Query Frame = 0


Query: 7   YLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLWCFYIYY 66
           Y +  V ETSFYNR+VL  + P    +P PH +QTWLRN + G ++YF SGFLWCFYIYY
Sbjct: 8   YNDQIVNETSFYNRMVLSHLLPVNLWEPLPHFLQTWLRNYLAGNILYFISGFLWCFYIYY 67

Query: 67  WKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRVLDVGWR 126
            K NVYVPK+SIP+ K ML Q++V +KAMP+Y +LP  +EY++EH WTKCY  +    W 
Sbjct: 68  LKLNVYVPKESIPTRKAMLLQIYVAMKAMPWYTLLPAVSEYMIEHGWTKCYSTLDHFNWF 127

Query: 127 AYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLAFHPVDG 186
               +I  YL  VEF IYW+H+ LHDIK LYK+LHA HH+YNK+ TLSPFAGLAFHP+DG
Sbjct: 128 LCFLYIALYLVLVEFMIYWVHKELHDIKFLYKHLHATHHMYNKQNTLSPFAGLAFHPLDG 187

Query: 187 ILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTIHHTRYR 246
           ILQAIPH+ ALF++P H  TH+ LLF E +WT +IHDCIH  IWP+MGAGYHTIHHT Y+
Sbjct: 188 ILQAIPHVIALFIVPIHLITHLSLLFLEGIWTASIHDCIHGNIWPIMGAGYHTIHHTTYK 247

Query: 247 YNYGHYSIWMDWMFGTLLDPI 268
           +NYGHY+IWMDWMFG+L+ P+
Sbjct: 248 HNYGHYTIWMDWMFGSLMVPL 268

[Close]

BLAST of CmaCh14G011780 vs. TAIR 10
Match: AT1G02205.2 (Fatty acid hydroxylase superfamily )

HSP 1 Score: 51.2 bits (121), Expect = 1.5e-06
Identity = 48/152 (31.58%), Postives = 72/152 (47.37%), Query Frame = 0


Query: 139 VEFCIYWMHRALHDIKPLYKYLHAKHH--IYNKKIT--LSPFAGLAFHPVDGILQAIPHL 198
           VEF  YW+H+ALH    LY   H+ HH  I  + IT  + PFA    H    IL AIP L
Sbjct: 138 VEFLYYWLHKALHH-HFLYSRYHSHHHSSIVTEPITSVIHPFAE---HIAYFILFAIPLL 197

Query: 199 ------------FALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVM-----GAGY 258
                       FA ++I   F  ++    FE++     H      ++P +        Y
Sbjct: 198 TTLLTKTASIISFAGYIIYIDFMNNMGHCNFELIPKRLFH------LFPPLKFLCYTPSY 257

Query: 259 HTIHHTRYRYNYGHYSIWMDWMFGTLLDPIDT 270
           H++HHT++R NY  +    D+++GT+ +  DT
Sbjct: 258 HSLHHTQFRTNYSLFMPLYDYIYGTMDESTDT 279

[Close]

BLAST of CmaCh14G011780 vs. TAIR 10
Match: AT1G02205.3 (Fatty acid hydroxylase superfamily )

HSP 1 Score: 51.2 bits (121), Expect = 1.5e-06
Identity = 48/152 (31.58%), Postives = 72/152 (47.37%), Query Frame = 0


Query: 139 VEFCIYWMHRALHDIKPLYKYLHAKHH--IYNKKIT--LSPFAGLAFHPVDGILQAIPHL 198
           VEF  YW+H+ALH    LY   H+ HH  I  + IT  + PFA    H    IL AIP L
Sbjct: 138 VEFLYYWLHKALHH-HFLYSRYHSHHHSSIVTEPITSVIHPFAE---HIAYFILFAIPLL 197

Query: 199 ------------FALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVM-----GAGY 258
                       FA ++I   F  ++    FE++     H      ++P +        Y
Sbjct: 198 TTLLTKTASIISFAGYIIYIDFMNNMGHCNFELIPKRLFH------LFPPLKFLCYTPSY 257

Query: 259 HTIHHTRYRYNYGHYSIWMDWMFGTLLDPIDT 270
           H++HHT++R NY  +    D+++GT+ +  DT
Sbjct: 258 HSLHHTQFRTNYSLFMPLYDYIYGTMDESTDT 279

[Close]

BLAST of CmaCh14G011780 vs. TAIR 10
Match: AT1G69640.1 (sphingoid base hydroxylase 1 )

HSP 1 Score: 48.1 bits (113), Expect = 1.3e-05
Identity = 44/155 (28.39%), Postives = 74/155 (47.74%), Query Frame = 0


Query: 129 AFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLAFHPVDG-I 188
           A   VT +  ++   Y+MHR +H  K LYK++H++HH   + I    +  L  HPV+G +
Sbjct: 96  ARQFVTAMIVLDTWQYFMHRYMHQNKFLYKHIHSQHH---RLIVPYAYGALYNHPVEGLL 155

Query: 189 LQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDC---IHAQIWPVM---GAGYHTIH 248
           L  I    +  +     RT I    F  + T + H C   +   ++ ++    + YH IH
Sbjct: 156 LDTIGGALSFLVSGMSPRTSIFFFSFATIKTVDDH-CGLWLPGNLFHMVFKNNSAYHDIH 215

Query: 249 H----TRYRYNYGHYSIWMDWMFGTLLDPIDTEAR 273
           H    T+Y ++   + +W D + GT + P   E R
Sbjct: 216 HQLYGTKYNFSQPFFVMW-DRILGTYM-PYSLEKR 244

The following BLAST results are available for this feature:

BLAST of CmaCh14G011780 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZT29	2.8e-114	67.16	Delta(7)-sterol-C5(6)-desaturase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q39208	1.9e-110	67.42	Delta(7)-sterol-C5(6)-desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=STE1 PE=1 ...	[more]
Q9M883	1.6e-101	62.45	Putative Delta(7)-sterol-C5(6)-desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=H...	[more]
Q9EQS5	1.6e-24	33.76	Lathosterol oxidase OS=Rattus norvegicus OX=10116 GN=Sc5d PE=1 SV=1	[more]
O88822	3.9e-23	32.48	Lathosterol oxidase OS=Mus musculus OX=10090 GN=Sc5d PE=2 SV=2	[more]

BLAST of CmaCh14G011780 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IX84	4.3e-166	100.00	delta(7)-sterol-C5(6)-desaturase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1GYF7	1.2e-165	99.27	delta(7)-sterol-C5(6)-desaturase-like OS=Cucurbita moschata OX=3662 GN=LOC111457...	[more]
A0A0A0L3B9	1.6e-152	90.15	Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A1S3BAC7	2.5e-150	89.38	delta(7)-sterol-C5(6)-desaturase-like OS=Cucumis melo OX=3656 GN=LOC103487532 PE...	[more]
A0A6J1KDR9	5.8e-147	87.78	delta(7)-sterol-C5(6)-desaturase-like OS=Cucurbita maxima OX=3661 GN=LOC11149293...	[more]

BLAST of CmaCh14G011780 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022980690.1	8.8e-166	100.00	delta(7)-sterol-C5(6)-desaturase-like isoform X1 [Cucurbita maxima]	[more]
XP_022956224.1	2.6e-165	99.27	delta(7)-sterol-C5(6)-desaturase-like [Cucurbita moschata] >KAG6581752.1 hypothe...	[more]
XP_023527022.1	2.6e-165	99.27	delta(7)-sterol-C5(6)-desaturase-like [Cucurbita pepo subsp. pepo]	[more]
XP_038894105.1	1.2e-154	90.88	delta(7)-sterol-C5(6)-desaturase-like [Benincasa hispida] >XP_038894106.1 delta(...	[more]
XP_004150515.1	3.2e-152	90.15	delta(7)-sterol-C5(6)-desaturase [Cucumis sativus] >KGN54626.1 hypothetical prot...	[more]

BLAST of CmaCh14G011780 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G02580.1	1.3e-111	67.42	sterol 1	[more]
AT3G02590.1	1.1e-102	62.45	Fatty acid hydroxylase superfamily protein	[more]
AT1G02205.2	1.5e-06	31.58	Fatty acid hydroxylase superfamily	[more]
AT1G02205.3	1.5e-06	31.58	Fatty acid hydroxylase superfamily	[more]
AT1G69640.1	1.3e-05	28.39	sphingoid base hydroxylase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 133..262 e-value: 1.2E-20 score: 74.2
None	No IPR available	PANTHER	PTHR11863:SF160	DELTA(7)-STEROL-C5(6)-DESATURASE	coord: 9..272
None	No IPR available	PANTHER	PTHR11863	STEROL DESATURASE	coord: 9..272

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh14G011780.1	CmaCh14G011780.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016126	sterol biosynthetic process
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0000248	C-5 sterol desaturase activity
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity