CmaCh14G010290 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh14G010290
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionprotein N-terminal asparagine amidohydrolase isoform X1
LocationCma_Chr14: 5418358 .. 5421893 (-)
RNA-Seq ExpressionCmaCh14G010290
SyntenyCmaCh14G010290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACATGAATAAACAAAAGCAAAACCCCCTGAAAGCCCATTTTTCCTCTCGAGCAATTCATACCATAATTTCCAGAAATCCTTTTACTCTTCTTCTCTCCCCCGTGATTCTTCATCTCTCTTCTCCTTCAATCTTCCATCCCAGAGCCATGATTTTCGTCGATGGAATACCCTTCACCGTCGAATCATCCTCGTCAAATAAGGTACTTCCCTGCTTCAATCTTCACTGTTTTTCATTTCCCTCTCTCCCTAATTTTCACTTTAAGTTATGAACCCTCTAGCTTATGTTTAAGTTTCCTCTCGTTTTTCCATTTCTGTGTTTAATTTTTTATGAAATCGATTCAGAAATTGCCCCACTTTCTTGTTTAACGATGTGGGCATTTTCAATTCTATTAGCAGAGGAACATGAGCTTGGATGGATTTCGAGCTATTCAGTAATATAAATTTGTTGTTGACTGTTCTCTGATTGTATTTTCCACGATCAAGTGTGTGTACACTGTTTGACTTCCAGTGCTTAACTTTGTAATGATTCATCTGCTCATATTTGCTTGATCATTATCGTTTTTCTTGATCTTTGACCTAGTAAACGGTTTTTTTACTGATTAATTTTCTGAATATGAGTGGGTTTGAGAACATATTCAAGATCTATCTAGCATGTTAGCATCTGATGGGCAAATTTTTTCTTTTAAATTAGATCACAAGCTTTAGGCATCCGGCAGCCCATGCTTGGACAAAGTTGTATTTGTTTAACCATTCAGAAAGAACTGTGTTTCTGAGCAGGGAGCTGATGTGCTGTATGCTTTAATGGAGTGTCCTTTTCTTGTTGATGCCACAAAATCATTCAAGAGTATTCCAGAGATGAGGTTCACAGATTCTGAACAGTTTGGTGTGGAGAGATCAACAATTTGTAAATGGGTTTATGTGTTTCAGAAAGAATATGCCACAGTAGATCCTGCCTTGGTGGATGTATTTTTCTCCTCTCCCTCTCTTTGGTTAAACTGCTCTTTCATGAATTTGAATTATGACCTTTGTTACTTAAACTTACTGGATAATTGATATGAATAGCTGCGTAGGGACTTCAAGCACTGTGTTTTCAAAAGCTTCCTGTCGAACCGTCTTCTAGTAGTTGTAGCCAAACATCTAATGCTCCTGTGTCTCAACCACGACCTTACTTGAACAATATCAATGTTATAGGTTGTACAACTATGTCGTTGGGTTTTAAAAAGTTATAACGCTTTTGTGTCCCATGAGATGTCAATAATACCCCGAGGTTAGGTTTTCGAGATCACGAAAATCCTCCGAAAGATGCCAAAAGATTATGATATTTATCCTTAGGACTATAACAAAGAACCTAAAGCAAGTTATTCCTTTACACATCCATAAGTATTTTCAATTGCATTCTGAATTAAAGTTGGTGATCAGCTGGGTAAGAAGTATTAGATCCTGATGTTGAAGCTTTCTGTTCTTTTCTAACTTTGTGTATTTATACCCACAATGCATAACTAAAGTTCGTTGGCACTGATGAAGCAACAACTTGTGTGGGCGTAGCTTTTCGGAATCGAAGAAACGGAATGTAAGAATTATTTCTGAGAAGTTATATGTATTTTCTTCTGCTAATCTCGCCATTATGCTACGCTTGTTGCCAACTTATGCTTCCTAATCTCATTTCCTGGCCTTCAGGACATCGGTTGCTCATATAGATTTTCCAAACATCATAGAAAACGCCGTCTCGCAGATGTTATCCTTAGTTGTCGATCCCTCTTCTGATGCCGAGTTAGATGTAATGTCCATAATGTCAATCCTGTTTATCTAACAAACGAATTGATTTAAGTTAATGCTTGAGGACTGATTCAAATCTATTACATAATGTAGGTTCATTTAGTTGGAGGCTTTGAAGATGTTCTACTCAAAGTCTGGCCCAGAACCAATAACTTTGTGATTCATTTCCAAGGATGCCATCCACACAATCAGAATATTTGAATTGAATTGTATGCATTGGGGTTTGATGCAGGAAAGTAATGATATCACCGAGTTGGAAGATCGTAAAAAGACAGAGGGTTATTCATTTCCGCTGTGTAACAAAATTATCGAGCGTTTATGGACAAGACCGGAGACATTTCATCTTCAGACTCTTTGTGTTCTTCATCACAACACTCGGAGGGACTCTAAAGGGAATGCTTACCCCATCTTCAGTGGATTCTCGGTTAGTCTTTGATTCATCCAGTTTCATGTTCTGTTTATTTTCTTTCATCTTTTCGGGTTGAGCTATGATTTTCATCACAATTCACGAGCTGATTTACGAGCTATAAGATGATCAGCATTGTTTGAGTGCGTTCTGTAAAACAAATTAAACCTTTAGAATGCAAATTTAACTTAAATGTGCTACTTTTCCTTTTGGTAATTCAGGTGAAAACTTCCGATGGATCTGTGTTCCCTGCTAGCTTTGACAGTACTTCAAGATGTCCAGATGAAGTTATTCGGAGAATTCGACTATCTTCCTCGTACGAGGATCCAAGTTTGAAGGGCAGACTGCTGGAGACATACGAAACTCATACCGATCAGTTCAAAATCGAACCATGCTGCTGGTAATCTGTCAACCTGTAACTTGCAAATCCTCCCCATGATCATTTTTACCTTACTATTAACTCCGCTGGACAACGAATGATGTTCTTGTGATATAATAGGTCGCCATGGAAACGATACATGGCTCTGTCTCTGCAACAGCTTTCCGATGCCGAAATCCTTCAATCGTGTTCTACCTCCCCTTCTGCAGAAGCACCAGACTTTGTGGAAAATTCAAGAAGGTATATATAACATGTCGAATACCCTCAAAATAAGATGTATTTTACTCGTCTCTGCAATCATATGAATTTCAAAACGTTCTTCCAATCATTCGTATACCCGTTTCCCCCCATGAATGTTCATACGACTCGATCTATTAAAACCAAATGCTGCTCGTATTGAATATGTGGAAATGAATATGATCTCCCTCTCTAACAGGCAGTGGGCTTATTTAGTGGAACACCCAGATTGGAAAGAAAGCTTCCCAAAGAAGAAGCCAAGAATTTTTAGAAGGGCTGCTGATGGGAAATGGGAAAGGAGCTTTAACTGACTTCTTCTCTGCTGTTATCTTTATGTGAATCCTTTTAGTTCTGTTATTTTATATTAATGGCCAACATTGTTTAACATATTTCCATTTCAAACCGAAGGGAGTTCGATGATTTGAAAGGCTTGATAAAGCTTTGGCATGACGTACACATGAGCAGGTTAGCAAGGGAAGGAGGAGACGATAGATGTTTAGTGCTATGGTCCGATAGAGAACTCGGATGAGAACGTTGGAGGAACAAAACTCATTGTTTTGTTACGCTTCAATGACGTGTTGAGTCAGATGAGGGAAAAATAAGAGGCAAGGTCATTTTCTTATCCAAAAAGGAAAACCACTAGTTCCACTTTCAGCTTATAGATATAACAAGTAGAAGAAAAAGTATATGCTAAGCTCACTTTGGTTGGATTTAAAAGTCTTTATCTTAATCTTTTT

mRNA sequence

AACATGAATAAACAAAAGCAAAACCCCCTGAAAGCCCATTTTTCCTCTCGAGCAATTCATACCATAATTTCCAGAAATCCTTTTACTCTTCTTCTCTCCCCCGTGATTCTTCATCTCTCTTCTCCTTCAATCTTCCATCCCAGAGCCATGATTTTCGTCGATGGAATACCCTTCACCGTCGAATCATCCTCGTCAAATAAGGGAGCTGATGTGCTGTATGCTTTAATGGAGTGTCCTTTTCTTGTTGATGCCACAAAATCATTCAAGAGTATTCCAGAGATGAGGTTCACAGATTCTGAACAGTTTGGTGTGGAGAGATCAACAATTTGTAAATGGGTTTATGTGTTTCAGAAAGAATATGCCACAGTAGATCCTGCCTTGGTGGATTTCGTTGGCACTGATGAAGCAACAACTTGTGTGGGCGTAGCTTTTCGGAATCGAAGAAACGGAATGACATCGGTTGCTCATATAGATTTTCCAAACATCATAGAAAACGCCGTCTCGCAGATGTTATCCTTAGTTGTCGATCCCTCTTCTGATGCCGAGTTAGATGTTCATTTAGTTGGAGGCTTTGAAGATGTTCTACTCAAAGTCTGGCCCAGAACCAATAACTTTGAAAGTAATGATATCACCGAGTTGGAAGATCGTAAAAAGACAGAGGGTTATTCATTTCCGCTGTGTAACAAAATTATCGAGCGTTTATGGACAAGACCGGAGACATTTCATCTTCAGACTCTTTGTGTTCTTCATCACAACACTCGGAGGGACTCTAAAGGGAATGCTTACCCCATCTTCAGTGGATTCTCGGTGAAAACTTCCGATGGATCTGTGTTCCCTGCTAGCTTTGACAGTACTTCAAGATGTCCAGATGAAGTTATTCGGAGAATTCGACTATCTTCCTCGTACGAGGATCCAAGTTTGAAGGGCAGACTGCTGGAGACATACGAAACTCATACCGATCAGTTCAAAATCGAACCATGCTGCTGGTCGCCATGGAAACGATACATGGCTCTGTCTCTGCAACAGCTTTCCGATGCCGAAATCCTTCAATCGTGTTCTACCTCCCCTTCTGCAGAAGCACCAGACTTTGTGGAAAATTCAAGAAGGCAGTGGGCTTATTTAGTGGAACACCCAGATTGGAAAGAAAGCTTCCCAAAGAAGAAGCCAAGAATTTTTAGAAGGGCTGCTGATGGGAAATGGGAAAGGAGCTTTAACTGACTTCTTCTCTGCTGTTATCTTTATGTGAATCCTTTTAGTTCTGTTATTTTATATTAATGGCCAACATTGTTTAACATATTTCCATTTCAAACCGAAGGGAGTTCGATGATTTGAAAGGCTTGATAAAGCTTTGGCATGACGTACACATGAGCAGGTTAGCAAGGGAAGGAGGAGACGATAGATGTTTAGTGCTATGGTCCGATAGAGAACTCGGATGAGAACGTTGGAGGAACAAAACTCATTGTTTTGTTACGCTTCAATGACGTGTTGAGTCAGATGAGGGAAAAATAAGAGGCAAGGTCATTTTCTTATCCAAAAAGGAAAACCACTAGTTCCACTTTCAGCTTATAGATATAACAAGTAGAAGAAAAAGTATATGCTAAGCTCACTTTGGTTGGATTTAAAAGTCTTTATCTTAATCTTTTT

Coding sequence (CDS)

ATGAATAAACAAAAGCAAAACCCCCTGAAAGCCCATTTTTCCTCTCGAGCAATTCATACCATAATTTCCAGAAATCCTTTTACTCTTCTTCTCTCCCCCGTGATTCTTCATCTCTCTTCTCCTTCAATCTTCCATCCCAGAGCCATGATTTTCGTCGATGGAATACCCTTCACCGTCGAATCATCCTCGTCAAATAAGGGAGCTGATGTGCTGTATGCTTTAATGGAGTGTCCTTTTCTTGTTGATGCCACAAAATCATTCAAGAGTATTCCAGAGATGAGGTTCACAGATTCTGAACAGTTTGGTGTGGAGAGATCAACAATTTGTAAATGGGTTTATGTGTTTCAGAAAGAATATGCCACAGTAGATCCTGCCTTGGTGGATTTCGTTGGCACTGATGAAGCAACAACTTGTGTGGGCGTAGCTTTTCGGAATCGAAGAAACGGAATGACATCGGTTGCTCATATAGATTTTCCAAACATCATAGAAAACGCCGTCTCGCAGATGTTATCCTTAGTTGTCGATCCCTCTTCTGATGCCGAGTTAGATGTTCATTTAGTTGGAGGCTTTGAAGATGTTCTACTCAAAGTCTGGCCCAGAACCAATAACTTTGAAAGTAATGATATCACCGAGTTGGAAGATCGTAAAAAGACAGAGGGTTATTCATTTCCGCTGTGTAACAAAATTATCGAGCGTTTATGGACAAGACCGGAGACATTTCATCTTCAGACTCTTTGTGTTCTTCATCACAACACTCGGAGGGACTCTAAAGGGAATGCTTACCCCATCTTCAGTGGATTCTCGGTGAAAACTTCCGATGGATCTGTGTTCCCTGCTAGCTTTGACAGTACTTCAAGATGTCCAGATGAAGTTATTCGGAGAATTCGACTATCTTCCTCGTACGAGGATCCAAGTTTGAAGGGCAGACTGCTGGAGACATACGAAACTCATACCGATCAGTTCAAAATCGAACCATGCTGCTGGTCGCCATGGAAACGATACATGGCTCTGTCTCTGCAACAGCTTTCCGATGCCGAAATCCTTCAATCGTGTTCTACCTCCCCTTCTGCAGAAGCACCAGACTTTGTGGAAAATTCAAGAAGGCAGTGGGCTTATTTAGTGGAACACCCAGATTGGAAAGAAAGCTTCCCAAAGAAGAAGCCAAGAATTTTTAGAAGGGCTGCTGATGGGAAATGGGAAAGGAGCTTTAACTGA

Protein sequence

MNKQKQNPLKAHFSSRAIHTIISRNPFTLLLSPVILHLSSPSIFHPRAMIFVDGIPFTVESSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTICKWVYVFQKEYATVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTNNFESNDITELEDRKKTEGYSFPLCNKIIERLWTRPETFHLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN
Homology
[Close]
BLAST of CmaCh14G010290 vs. ExPASy Swiss-Prot
Match: O64876 (Protein N-terminal asparagine amidohydrolase OS=Arabidopsis thaliana OX=3702 GN=NTAN1 PE=2 SV=2)

HSP 1 Score: 369.8 bits (948), Expect = 4.0e-101
Identity = 185/358 (51.68%), Postives = 250/358 (69.83%), Query Frame = 0 

Query: 49  MIFVDGIPFTVES-----SSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGV 108
           MI+V G+ F  ES     SSS++G+ +L  +M  P +  A+ SFK++ E   +  E    
Sbjct: 1   MIYVGGVQFLDESSSFSLSSSSQGSSLLVDVMSHPVITLASDSFKNLEEKNVSFDES-DS 60

Query: 109 ERSTICKWVYVFQKEYATVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIE 168
           E ST  ++VY+FQ+E+A V+PALVDFVGTDEATTCVG+  RNR++GMTSVAH+D P I++
Sbjct: 61  ESSTKDRYVYIFQREFAVVNPALVDFVGTDEATTCVGLVIRNRKSGMTSVAHMDSPEIVD 120

Query: 169 NAVSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTNNFESNDITELEDRKKTEGYSF 228
             +SQML LV+    DAELDVH+VGG+EDV +K           +   + D  K EGYSF
Sbjct: 121 LGISQMLLLVLQDDVDAELDVHMVGGYEDVDIK-----------NADGVGDYAKPEGYSF 180

Query: 229 PLCNKIIERLWTRPETFHLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDS 288
           PLC K++E L  R E FH+QTL +L HNT+ DS+ N  PIF+G  V TS G++ PASF+ 
Sbjct: 181 PLCCKLVETLQKRRENFHIQTLFILGHNTKLDSQANTCPIFNGCLVNTSTGAILPASFNR 240

Query: 289 TSRCPDEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLS 348
           TSRCPDE++RRIR+SSS+ED S KG+LL+TY+T TD+F I PC W+         L QL+
Sbjct: 241 TSRCPDEIVRRIRVSSSFEDSSWKGKLLDTYDTKTDRFIIAPCRWTMRLIEYVWELNQLT 300

Query: 349 DAEILQSCSTSPSAEAPDFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWER 402
           D EIL +CSTSPSAE PDFV + RR W YL+++P+W ++FP+++PR+F R  DG W++
Sbjct: 301 DEEILTNCSTSPSAEGPDFVNSLRRNWGYLLKYPEWSKTFPRRQPRVFERTVDGHWKK 346

[Close]
BLAST of CmaCh14G010290 vs. ExPASy Swiss-Prot
Match: Q64311 (Protein N-terminal asparagine amidohydrolase OS=Mus musculus OX=10090 GN=Ntan1 PE=1 SV=3)

HSP 1 Score: 124.8 bits (312), Expect = 2.2e-27
Identity = 93/296 (31.42%), Postives = 146/296 (49.32%), Query Frame = 0 

Query: 112 VYVFQKEYATVDP--ALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQM 171
           +YV Q+E A   P    +  +G+D+ATTC  V  R+  NG T + H D  +  +  V  +
Sbjct: 47  LYVQQRELAVTSPKDGSISILGSDDATTCHIVVLRHTGNGATCLTHCDGSD-TKAEVPLI 106

Query: 172 LSLVVDPSSDAE---LDVHLVGGFEDVLLKVWPRTNNFESNDITELEDRKKTEGYSFPLC 231
           +S +   S  AE   L+VHLVGGF D                     DR+ ++     L 
Sbjct: 107 MSSIKSFSEHAECGRLEVHLVGGFSD---------------------DRQLSQ----KLT 166

Query: 232 NKIIERLWTRPETFHLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSR 291
           ++++     + +  HL TLCV   N R +++ N +PI  G +V      ++ ASF    R
Sbjct: 167 HQLLSEFDKQDDDIHLVTLCVTELNDREENE-NHFPIIYGIAVNIKTAEIYRASFQ--DR 226

Query: 292 CPDEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAE 351
            P+E +R  R  +        G ++  Y+  T+Q +I PC W+P+ + +   LQQ  D +
Sbjct: 227 GPEEQLRAARALAG-------GPMISIYDAKTEQLRIGPCSWTPFPQ-VDFWLQQ-DDKQ 286

Query: 352 ILQSCSTSPSAEAPDFVENSRRQWAYLVEHPDWKE-SFPKKKPRIFRRAADGKWER 402
           IL+S STSP AE P FVE+ R    +L + P  +   FP  K  ++++  DG WE+
Sbjct: 287 ILESLSTSPLAEPPHFVEHIRSTLMFLKKFPSPENILFPGNKALLYKKNKDGLWEK 304

[Close]
BLAST of CmaCh14G010290 vs. ExPASy Swiss-Prot
Match: Q28955 (Protein N-terminal asparagine amidohydrolase OS=Sus scrofa OX=9823 GN=NTAN1 PE=1 SV=4)

HSP 1 Score: 118.2 bits (295), Expect = 2.1e-25
Identity = 90/295 (30.51%), Postives = 145/295 (49.15%), Query Frame = 0 

Query: 112 VYVFQKEYATVDP--ALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQM 171
           +YV Q+E A   P    V  +G+D+ATTC  V  R+  NG T + H D  +  +  VS +
Sbjct: 47  LYVQQRELAVTSPKDGSVCILGSDDATTCHIVVLRHTGNGATCLTHCDGTD-TKAEVSLI 106

Query: 172 LSLV---VDPSSDAELDVHLVGGFEDVLLKVWPRTNNFESNDITELEDRKKTEGYSFPLC 231
           +S +    D +    L+VHLVGGF D                     DR+ ++     L 
Sbjct: 107 MSSIKSFSDHTQRGRLEVHLVGGFSD---------------------DRQLSQ----KLT 166

Query: 232 NKIIERLWTRPETFHLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSR 291
           ++++     + +  HL TLCV   N R +++ N +PI  G +V      ++ ASF    R
Sbjct: 167 HQLLSEFDRQEDDIHLVTLCVTELNDREENE-NHFPIIYGIAVNVKTAEIYRASF--PDR 226

Query: 292 CPDEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAE 351
            P+E +R  R+ +        G ++  Y+  T+Q +I P  W P+  ++   LQQ  D +
Sbjct: 227 GPEEELRAARVLTG-------GPMISIYDAKTEQLRIGPYSWMPFP-HVDFWLQQ-DDKQ 286

Query: 352 ILQSCSTSPSAEAPDFVENSRRQWAYLVEHPDWKES-FPKKKPRIFRRAADGKWE 401
           IL++ STSP AE P FVE+ R    +L ++P    + FP  K  ++++  DG W+
Sbjct: 287 ILENLSTSPLAEPPHFVEHIRSTLMFLKKYPSPTNTLFPGNKALLYKKNEDGLWK 303

[Close]
BLAST of CmaCh14G010290 vs. ExPASy Swiss-Prot
Match: Q96AB6 (Protein N-terminal asparagine amidohydrolase OS=Homo sapiens OX=9606 GN=NTAN1 PE=1 SV=3)

HSP 1 Score: 116.7 bits (291), Expect = 6.1e-25
Identity = 85/295 (28.81%), Postives = 139/295 (47.12%), Query Frame = 0 

Query: 112 VYVFQKEYATVDP--ALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENA--VS 171
           +YV Q+E A   P    +  +G+D+ATTC  V  R+  NG T + H D  +       + 
Sbjct: 47  LYVQQRELAVTSPKDGSISILGSDDATTCHIVVLRHTGNGATCLTHCDGTDTKAEVPLIM 106

Query: 172 QMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTNNFESNDITELEDRKKTEGYSFPLCN 231
             +    D +    L+VHLVGGF D                     DR+ ++     L +
Sbjct: 107 NSIKSFSDHAQCGRLEVHLVGGFSD---------------------DRQLSQ----KLTH 166

Query: 232 KIIERLWTRPETFHLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRC 291
           +++     + +  HL TLCV   N R +++ N +P+  G +V      ++ ASF    R 
Sbjct: 167 QLLSEFDRQEDDIHLVTLCVTELNDREENE-NHFPVIYGIAVNIKTAEIYRASFQ--DRG 226

Query: 292 PDEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEI 351
           P+E +R  R  +        G ++  Y+  T+Q +I P  W+P+  ++   L Q  D +I
Sbjct: 227 PEEQLRAARTLAG-------GPMISIYDAETEQLRIGPYSWTPFP-HVDFWLHQ-DDKQI 286

Query: 352 LQSCSTSPSAEAPDFVENSRRQWAYLVEHPDWKES-FPKKKPRIFRRAADGKWER 402
           L++ STSP AE P FVE+ R    +L +HP    + F   K  ++++  DG WE+
Sbjct: 287 LENLSTSPLAEPPHFVEHIRSTLMFLKKHPSPAHTLFSGNKALLYKKNEDGLWEK 304

[Close]
BLAST of CmaCh14G010290 vs. ExPASy TrEMBL
Match: A0A6J1J4Y8 (protein N-terminal asparagine amidohydrolase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481410 PE=4 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 8.9e-229
Identity = 396/404 (98.02%), Postives = 396/404 (98.02%), Query Frame = 0 

Query: 1   MNKQKQNPLKAHFSSRAIHTIISRNPFTLLLSPVILHLSSPSIFHPRAMIFVDGIPFTVE 60
           MNKQKQNPLKAHFSSRAIHTIISRNPFTLLLSPVILHLSSPSIFHPRAMIFVDGIPFTVE
Sbjct: 1   MNKQKQNPLKAHFSSRAIHTIISRNPFTLLLSPVILHLSSPSIFHPRAMIFVDGIPFTVE 60

Query: 61  SSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTICKWVYVFQKEYA 120
           SSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTICKWVYVFQKEYA
Sbjct: 61  SSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTICKWVYVFQKEYA 120

Query: 121 TVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQMLSLVVDPSSDA 180
           TVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQMLSLVVDPSSDA
Sbjct: 121 TVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQMLSLVVDPSSDA 180

Query: 181 ELDVHLVGGFEDVLLKVWPRTNNFESNDITELEDRKKTEGYSFPLCNKIIERLWTRPETF 240
           ELDVHLVGGFEDVLLK        ESNDITELEDRKKTEGYSFPLCNKIIERLWTRPETF
Sbjct: 181 ELDVHLVGGFEDVLLK--------ESNDITELEDRKKTEGYSFPLCNKIIERLWTRPETF 240

Query: 241 HLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSS 300
           HLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSS
Sbjct: 241 HLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSS 300

Query: 301 YEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAP 360
           YEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAP
Sbjct: 301 YEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAP 360

Query: 361 DFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN 405
           DFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN
Sbjct: 361 DFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN 396

[Close]
BLAST of CmaCh14G010290 vs. ExPASy TrEMBL
Match: A0A6J1IWZ0 (protein N-terminal asparagine amidohydrolase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481410 PE=4 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 8.3e-227
Identity = 396/410 (96.59%), Postives = 396/410 (96.59%), Query Frame = 0 

Query: 1   MNKQKQNPLKAHFSSRAIHTIISRNPFTLLLSPVILHLSSPSIFHPRAMIFVDGIPFTVE 60
           MNKQKQNPLKAHFSSRAIHTIISRNPFTLLLSPVILHLSSPSIFHPRAMIFVDGIPFTVE
Sbjct: 1   MNKQKQNPLKAHFSSRAIHTIISRNPFTLLLSPVILHLSSPSIFHPRAMIFVDGIPFTVE 60

Query: 61  SSSSNK------GADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTICKWVYV 120
           SSSSNK      GADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTICKWVYV
Sbjct: 61  SSSSNKNCVSEQGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTICKWVYV 120

Query: 121 FQKEYATVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQMLSLVV 180
           FQKEYATVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQMLSLVV
Sbjct: 121 FQKEYATVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQMLSLVV 180

Query: 181 DPSSDAELDVHLVGGFEDVLLKVWPRTNNFESNDITELEDRKKTEGYSFPLCNKIIERLW 240
           DPSSDAELDVHLVGGFEDVLLK        ESNDITELEDRKKTEGYSFPLCNKIIERLW
Sbjct: 181 DPSSDAELDVHLVGGFEDVLLK--------ESNDITELEDRKKTEGYSFPLCNKIIERLW 240

Query: 241 TRPETFHLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRR 300
           TRPETFHLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRR
Sbjct: 241 TRPETFHLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRR 300

Query: 301 IRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTS 360
           IRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTS
Sbjct: 301 IRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTS 360

Query: 361 PSAEAPDFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN 405
           PSAEAPDFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN
Sbjct: 361 PSAEAPDFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN 402

[Close]
BLAST of CmaCh14G010290 vs. ExPASy TrEMBL
Match: A0A6J1FB23 (protein N-terminal asparagine amidohydrolase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442418 PE=4 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 1.5e-196
Identity = 338/356 (94.94%), Postives = 347/356 (97.47%), Query Frame = 0 

Query: 49  MIFVDGIPFTVESSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTI 108
           MIFVDG+PFTVESSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERST+
Sbjct: 1   MIFVDGVPFTVESSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTM 60

Query: 109 CKWVYVFQKEYATVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQ 168
           CKWVY+FQKEYATVDPALVDFVGTDEATTCVGVA RNR+NGMTSVAHIDFPNIIENA+SQ
Sbjct: 61  CKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNRKNGMTSVAHIDFPNIIENALSQ 120

Query: 169 MLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTNNFESNDITELEDRKKTEGYSFPLCNK 228
           MLSLVVDPSSDAELDVHLVGGFEDVLLK        ESNDITELEDRKKTEG+SFPLCNK
Sbjct: 121 MLSLVVDPSSDAELDVHLVGGFEDVLLK--------ESNDITELEDRKKTEGHSFPLCNK 180

Query: 229 IIERLWTRPETFHLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCP 288
           IIERLWTRPETFHLQTLCVLHHNTRRDS+GNAYPIFSGFSVKTSDGSVFPASFDSTSRCP
Sbjct: 181 IIERLWTRPETFHLQTLCVLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCP 240

Query: 289 DEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEIL 348
           DEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEIL
Sbjct: 241 DEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEIL 300

Query: 349 QSCSTSPSAEAPDFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN 405
           QSCSTSPSAEAPDFVENSRRQWAYLVEHPDW+ESFP+KKPRIFRRAADGKWERSFN
Sbjct: 301 QSCSTSPSAEAPDFVENSRRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 348

[Close]
BLAST of CmaCh14G010290 vs. ExPASy TrEMBL
Match: A0A6J1F5V2 (protein N-terminal asparagine amidohydrolase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442418 PE=4 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 1.4e-194
Identity = 338/362 (93.37%), Postives = 347/362 (95.86%), Query Frame = 0 

Query: 49  MIFVDGIPFTVESSSSNK------GADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFG 108
           MIFVDG+PFTVESSSSNK      GADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFG
Sbjct: 1   MIFVDGVPFTVESSSSNKNCVSEQGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFG 60

Query: 109 VERSTICKWVYVFQKEYATVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNII 168
           VERST+CKWVY+FQKEYATVDPALVDFVGTDEATTCVGVA RNR+NGMTSVAHIDFPNII
Sbjct: 61  VERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNRKNGMTSVAHIDFPNII 120

Query: 169 ENAVSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTNNFESNDITELEDRKKTEGYS 228
           ENA+SQMLSLVVDPSSDAELDVHLVGGFEDVLLK        ESNDITELEDRKKTEG+S
Sbjct: 121 ENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLK--------ESNDITELEDRKKTEGHS 180

Query: 229 FPLCNKIIERLWTRPETFHLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFD 288
           FPLCNKIIERLWTRPETFHLQTLCVLHHNTRRDS+GNAYPIFSGFSVKTSDGSVFPASFD
Sbjct: 181 FPLCNKIIERLWTRPETFHLQTLCVLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFD 240

Query: 289 STSRCPDEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQL 348
           STSRCPDEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQL
Sbjct: 241 STSRCPDEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQL 300

Query: 349 SDAEILQSCSTSPSAEAPDFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERS 405
           SDAEILQSCSTSPSAEAPDFVENSRRQWAYLVEHPDW+ESFP+KKPRIFRRAADGKWERS
Sbjct: 301 SDAEILQSCSTSPSAEAPDFVENSRRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERS 354

[Close]
BLAST of CmaCh14G010290 vs. ExPASy TrEMBL
Match: A0A6J1J336 (protein N-terminal asparagine amidohydrolase isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111481410 PE=4 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 1.6e-185
Identity = 322/330 (97.58%), Postives = 322/330 (97.58%), Query Frame = 0 

Query: 75  MECPFLVDATKSFKSIPEMRFTDSEQFGVERSTICKWVYVFQKEYATVDPALVDFVGTDE 134
           MECPFLVDATKSFKSIPEMRFTDSEQFGVERSTICKWVYVFQKEYATVDPALVDFVGTDE
Sbjct: 1   MECPFLVDATKSFKSIPEMRFTDSEQFGVERSTICKWVYVFQKEYATVDPALVDFVGTDE 60

Query: 135 ATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQMLSLVVDPSSDAELDVHLVGGFEDVL 194
           ATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQMLSLVVDPSSDAELDVHLVGGFEDVL
Sbjct: 61  ATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQMLSLVVDPSSDAELDVHLVGGFEDVL 120

Query: 195 LKVWPRTNNFESNDITELEDRKKTEGYSFPLCNKIIERLWTRPETFHLQTLCVLHHNTRR 254
           LK        ESNDITELEDRKKTEGYSFPLCNKIIERLWTRPETFHLQTLCVLHHNTRR
Sbjct: 121 LK--------ESNDITELEDRKKTEGYSFPLCNKIIERLWTRPETFHLQTLCVLHHNTRR 180

Query: 255 DSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSLKGRLLETY 314
           DSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSLKGRLLETY
Sbjct: 181 DSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSLKGRLLETY 240

Query: 315 ETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENSRRQWAYLV 374
           ETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENSRRQWAYLV
Sbjct: 241 ETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENSRRQWAYLV 300

Query: 375 EHPDWKESFPKKKPRIFRRAADGKWERSFN 405
           EHPDWKESFPKKKPRIFRRAADGKWERSFN
Sbjct: 301 EHPDWKESFPKKKPRIFRRAADGKWERSFN 322

[Close]
BLAST of CmaCh14G010290 vs. NCBI nr
Match: XP_022982574.1 (protein N-terminal asparagine amidohydrolase isoform X2 [Cucurbita maxima])

HSP 1 Score: 802.4 bits (2071), Expect = 1.8e-228
Identity = 396/404 (98.02%), Postives = 396/404 (98.02%), Query Frame = 0 

Query: 1   MNKQKQNPLKAHFSSRAIHTIISRNPFTLLLSPVILHLSSPSIFHPRAMIFVDGIPFTVE 60
           MNKQKQNPLKAHFSSRAIHTIISRNPFTLLLSPVILHLSSPSIFHPRAMIFVDGIPFTVE
Sbjct: 1   MNKQKQNPLKAHFSSRAIHTIISRNPFTLLLSPVILHLSSPSIFHPRAMIFVDGIPFTVE 60

Query: 61  SSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTICKWVYVFQKEYA 120
           SSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTICKWVYVFQKEYA
Sbjct: 61  SSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTICKWVYVFQKEYA 120

Query: 121 TVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQMLSLVVDPSSDA 180
           TVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQMLSLVVDPSSDA
Sbjct: 121 TVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQMLSLVVDPSSDA 180

Query: 181 ELDVHLVGGFEDVLLKVWPRTNNFESNDITELEDRKKTEGYSFPLCNKIIERLWTRPETF 240
           ELDVHLVGGFEDVLLK        ESNDITELEDRKKTEGYSFPLCNKIIERLWTRPETF
Sbjct: 181 ELDVHLVGGFEDVLLK--------ESNDITELEDRKKTEGYSFPLCNKIIERLWTRPETF 240

Query: 241 HLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSS 300
           HLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSS
Sbjct: 241 HLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSS 300

Query: 301 YEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAP 360
           YEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAP
Sbjct: 301 YEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAP 360

Query: 361 DFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN 405
           DFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN
Sbjct: 361 DFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN 396

[Close]
BLAST of CmaCh14G010290 vs. NCBI nr
Match: XP_022982572.1 (protein N-terminal asparagine amidohydrolase isoform X1 [Cucurbita maxima])

HSP 1 Score: 795.8 bits (2054), Expect = 1.7e-226
Identity = 396/410 (96.59%), Postives = 396/410 (96.59%), Query Frame = 0 

Query: 1   MNKQKQNPLKAHFSSRAIHTIISRNPFTLLLSPVILHLSSPSIFHPRAMIFVDGIPFTVE 60
           MNKQKQNPLKAHFSSRAIHTIISRNPFTLLLSPVILHLSSPSIFHPRAMIFVDGIPFTVE
Sbjct: 1   MNKQKQNPLKAHFSSRAIHTIISRNPFTLLLSPVILHLSSPSIFHPRAMIFVDGIPFTVE 60

Query: 61  SSSSNK------GADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTICKWVYV 120
           SSSSNK      GADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTICKWVYV
Sbjct: 61  SSSSNKNCVSEQGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTICKWVYV 120

Query: 121 FQKEYATVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQMLSLVV 180
           FQKEYATVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQMLSLVV
Sbjct: 121 FQKEYATVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQMLSLVV 180

Query: 181 DPSSDAELDVHLVGGFEDVLLKVWPRTNNFESNDITELEDRKKTEGYSFPLCNKIIERLW 240
           DPSSDAELDVHLVGGFEDVLLK        ESNDITELEDRKKTEGYSFPLCNKIIERLW
Sbjct: 181 DPSSDAELDVHLVGGFEDVLLK--------ESNDITELEDRKKTEGYSFPLCNKIIERLW 240

Query: 241 TRPETFHLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRR 300
           TRPETFHLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRR
Sbjct: 241 TRPETFHLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRR 300

Query: 301 IRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTS 360
           IRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTS
Sbjct: 301 IRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTS 360

Query: 361 PSAEAPDFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN 405
           PSAEAPDFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN
Sbjct: 361 PSAEAPDFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN 402

[Close]
BLAST of CmaCh14G010290 vs. NCBI nr
Match: XP_022935590.1 (protein N-terminal asparagine amidohydrolase isoform X2 [Cucurbita moschata] >KAG6581602.1 Protein N-terminal asparagine amidohydrolase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 695.3 bits (1793), Expect = 3.2e-196
Identity = 338/356 (94.94%), Postives = 347/356 (97.47%), Query Frame = 0 

Query: 49  MIFVDGIPFTVESSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTI 108
           MIFVDG+PFTVESSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERST+
Sbjct: 1   MIFVDGVPFTVESSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTM 60

Query: 109 CKWVYVFQKEYATVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIENAVSQ 168
           CKWVY+FQKEYATVDPALVDFVGTDEATTCVGVA RNR+NGMTSVAHIDFPNIIENA+SQ
Sbjct: 61  CKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNRKNGMTSVAHIDFPNIIENALSQ 120

Query: 169 MLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTNNFESNDITELEDRKKTEGYSFPLCNK 228
           MLSLVVDPSSDAELDVHLVGGFEDVLLK        ESNDITELEDRKKTEG+SFPLCNK
Sbjct: 121 MLSLVVDPSSDAELDVHLVGGFEDVLLK--------ESNDITELEDRKKTEGHSFPLCNK 180

Query: 229 IIERLWTRPETFHLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCP 288
           IIERLWTRPETFHLQTLCVLHHNTRRDS+GNAYPIFSGFSVKTSDGSVFPASFDSTSRCP
Sbjct: 181 IIERLWTRPETFHLQTLCVLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCP 240

Query: 289 DEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEIL 348
           DEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEIL
Sbjct: 241 DEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEIL 300

Query: 349 QSCSTSPSAEAPDFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN 405
           QSCSTSPSAEAPDFVENSRRQWAYLVEHPDW+ESFP+KKPRIFRRAADGKWERSFN
Sbjct: 301 QSCSTSPSAEAPDFVENSRRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 348

[Close]
BLAST of CmaCh14G010290 vs. NCBI nr
Match: KAG7018102.1 (Protein N-terminal asparagine amidohydrolase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 694.9 bits (1792), Expect = 4.1e-196
Identity = 345/398 (86.68%), Postives = 354/398 (88.94%), Query Frame = 0 

Query: 49  MIFVDGIPFTVESSSSNK----------------------GADVLYALMECPFLVDATKS 108
           MIFVDG+PFTVESSSSNK                      GADVLYALMECPFLVDATKS
Sbjct: 1   MIFVDGVPFTVESSSSNKITSFTHPAAHAWTKLKNCVSEQGADVLYALMECPFLVDATKS 60

Query: 109 FKSIPEMRFTDSEQFGVERSTICKWVYVFQKEYATVDPALVDFVGTDEATTCVGVAFRNR 168
           FKSIPEMRFTDSEQFGVERST+CKWVY+FQKEYATVDPALVDFVGTDEATTCVGVA RNR
Sbjct: 61  FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR 120

Query: 169 RNGMTSVAHIDFPNIIENAVSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTNNF-- 228
           +NGMTSVAHIDFPNIIENA+SQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRT NF  
Sbjct: 121 KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTYNFDA 180

Query: 229 ------------------ESNDITELEDRKKTEGYSFPLCNKIIERLWTRPETFHLQTLC 288
                             ESNDITELEDRKKTEG+SFPLCNKIIERLWTRPETFHLQTLC
Sbjct: 181 IHTIRIFELNCMHWGLMQESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC 240

Query: 289 VLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 348
           VLHHNTRRDS+GNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL
Sbjct: 241 VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 300

Query: 349 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 405
           KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS
Sbjct: 301 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 360

[Close]
BLAST of CmaCh14G010290 vs. NCBI nr
Match: XP_022935589.1 (protein N-terminal asparagine amidohydrolase isoform X1 [Cucurbita moschata])

HSP 1 Score: 688.7 bits (1776), Expect = 3.0e-194
Identity = 338/362 (93.37%), Postives = 347/362 (95.86%), Query Frame = 0 

Query: 49  MIFVDGIPFTVESSSSNK------GADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFG 108
           MIFVDG+PFTVESSSSNK      GADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFG
Sbjct: 1   MIFVDGVPFTVESSSSNKNCVSEQGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFG 60

Query: 109 VERSTICKWVYVFQKEYATVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNII 168
           VERST+CKWVY+FQKEYATVDPALVDFVGTDEATTCVGVA RNR+NGMTSVAHIDFPNII
Sbjct: 61  VERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNRKNGMTSVAHIDFPNII 120

Query: 169 ENAVSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTNNFESNDITELEDRKKTEGYS 228
           ENA+SQMLSLVVDPSSDAELDVHLVGGFEDVLLK        ESNDITELEDRKKTEG+S
Sbjct: 121 ENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLK--------ESNDITELEDRKKTEGHS 180

Query: 229 FPLCNKIIERLWTRPETFHLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFD 288
           FPLCNKIIERLWTRPETFHLQTLCVLHHNTRRDS+GNAYPIFSGFSVKTSDGSVFPASFD
Sbjct: 181 FPLCNKIIERLWTRPETFHLQTLCVLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFD 240

Query: 289 STSRCPDEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQL 348
           STSRCPDEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQL
Sbjct: 241 STSRCPDEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQL 300

Query: 349 SDAEILQSCSTSPSAEAPDFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERS 405
           SDAEILQSCSTSPSAEAPDFVENSRRQWAYLVEHPDW+ESFP+KKPRIFRRAADGKWERS
Sbjct: 301 SDAEILQSCSTSPSAEAPDFVENSRRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERS 354

[Close]
BLAST of CmaCh14G010290 vs. TAIR 10
Match: AT2G44420.1 (protein N-terminal asparagine amidohydrolase family protein )

HSP 1 Score: 369.8 bits (948), Expect = 2.8e-102
Identity = 185/358 (51.68%), Postives = 250/358 (69.83%), Query Frame = 0 

Query: 49  MIFVDGIPFTVES-----SSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGV 108
           MI+V G+ F  ES     SSS++G+ +L  +M  P +  A+ SFK++ E   +  E    
Sbjct: 1   MIYVGGVQFLDESSSFSLSSSSQGSSLLVDVMSHPVITLASDSFKNLEEKNVSFDES-DS 60

Query: 109 ERSTICKWVYVFQKEYATVDPALVDFVGTDEATTCVGVAFRNRRNGMTSVAHIDFPNIIE 168
           E ST  ++VY+FQ+E+A V+PALVDFVGTDEATTCVG+  RNR++GMTSVAH+D P I++
Sbjct: 61  ESSTKDRYVYIFQREFAVVNPALVDFVGTDEATTCVGLVIRNRKSGMTSVAHMDSPEIVD 120

Query: 169 NAVSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTNNFESNDITELEDRKKTEGYSF 228
             +SQML LV+    DAELDVH+VGG+EDV +K           +   + D  K EGYSF
Sbjct: 121 LGISQMLLLVLQDDVDAELDVHMVGGYEDVDIK-----------NADGVGDYAKPEGYSF 180

Query: 229 PLCNKIIERLWTRPETFHLQTLCVLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDS 288
           PLC K++E L  R E FH+QTL +L HNT+ DS+ N  PIF+G  V TS G++ PASF+ 
Sbjct: 181 PLCCKLVETLQKRRENFHIQTLFILGHNTKLDSQANTCPIFNGCLVNTSTGAILPASFNR 240

Query: 289 TSRCPDEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLS 348
           TSRCPDE++RRIR+SSS+ED S KG+LL+TY+T TD+F I PC W+         L QL+
Sbjct: 241 TSRCPDEIVRRIRVSSSFEDSSWKGKLLDTYDTKTDRFIIAPCRWTMRLIEYVWELNQLT 300

Query: 349 DAEILQSCSTSPSAEAPDFVENSRRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWER 402
           D EIL +CSTSPSAE PDFV + RR W YL+++P+W ++FP+++PR+F R  DG W++
Sbjct: 301 DEEILTNCSTSPSAEGPDFVNSLRRNWGYLLKYPEWSKTFPRRQPRVFERTVDGHWKK 346

The following BLAST results are available for this feature:
BLAST of CmaCh14G010290 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O648764.0e-10151.68Protein N-terminal asparagine amidohydrolase OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q643112.2e-2731.42Protein N-terminal asparagine amidohydrolase OS=Mus musculus OX=10090 GN=Ntan1 P... [more]
Q289552.1e-2530.51Protein N-terminal asparagine amidohydrolase OS=Sus scrofa OX=9823 GN=NTAN1 PE=1... [more]
Q96AB66.1e-2528.81Protein N-terminal asparagine amidohydrolase OS=Homo sapiens OX=9606 GN=NTAN1 PE... [more]
BLAST of CmaCh14G010290 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1J4Y88.9e-22998.02protein N-terminal asparagine amidohydrolase isoform X2 OS=Cucurbita maxima OX=3... [more]
A0A6J1IWZ08.3e-22796.59protein N-terminal asparagine amidohydrolase isoform X1 OS=Cucurbita maxima OX=3... [more]
A0A6J1FB231.5e-19694.94protein N-terminal asparagine amidohydrolase isoform X2 OS=Cucurbita moschata OX... [more]
A0A6J1F5V21.4e-19493.37protein N-terminal asparagine amidohydrolase isoform X1 OS=Cucurbita moschata OX... [more]
A0A6J1J3361.6e-18597.58protein N-terminal asparagine amidohydrolase isoform X3 OS=Cucurbita maxima OX=3... [more]
BLAST of CmaCh14G010290 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022982574.11.8e-22898.02protein N-terminal asparagine amidohydrolase isoform X2 [Cucurbita maxima][more]
XP_022982572.11.7e-22696.59protein N-terminal asparagine amidohydrolase isoform X1 [Cucurbita maxima][more]
XP_022935590.13.2e-19694.94protein N-terminal asparagine amidohydrolase isoform X2 [Cucurbita moschata] >KA... [more]
KAG7018102.14.1e-19686.68Protein N-terminal asparagine amidohydrolase [Cucurbita argyrosperma subsp. argy... [more]
XP_022935589.13.0e-19493.37protein N-terminal asparagine amidohydrolase isoform X1 [Cucurbita moschata][more]
BLAST of CmaCh14G010290 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G44420.12.8e-10251.68protein N-terminal asparagine amidohydrolase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026750Protein N-terminal asparagine amidohydrolasePFAMPF14736N_Asn_amidohydcoord: 106..401
e-value: 2.0E-92
score: 309.2
IPR026750Protein N-terminal asparagine amidohydrolasePANTHERPTHR12498N-TERMINAL ASPARAGINE AMIDOHYDROLASEcoord: 49..401

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh14G010290.1CmaCh14G010290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0008418 protein-N-terminal asparagine amidohydrolase activity
molecular_function GO:0016740 transferase activity