CmaCh14G009760 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh14G009760
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Hydroxymethylbilane synthase
Location	Cma_Chr14: 5124245 .. 5127264 (-)
RNA-Seq Expression	CmaCh14G009760
Synteny	CmaCh14G009760

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAATCCTATCTGCCAACGCGAATGCAGACATTTGGCCTGTTGTGTCATCAAAAACAATCCCTTCTAAGCTAACCCATCTCCTTTTTCTTTCTTCTTCTTCTTCTTCTTCTTCGTCTTCTCAAATGGTTGCTTTATCTTCCCCTTCTGTAAGCAACTCTCCCATGGCTCGCCCTTGTAATCTTGCCTCCCTTTCTCTTCCTGGGTTCTCCTCACTTTCCCTTAAACCTCCGGCTTTCACTAATGCCCCCAAGAAATTTCATGGGTTTGGCCTCATAAGAGCCTCTGTCGCTGTGGAGCAGCAGCCAGAGAAGACCAAGGTTGCTCTCCTCAGAATTGGCACCAGAGGAAGGTATAACTTTTTTGTTTTGTGGGTCTTTTTTAGATTTTCTTTTGGTTTTATGCTTTAGGATGTCCTTAGTTTCTGGTCATGAGTTTGTGGCTGATTTGAGGGATAATGTAGGGCATTGAATTGTTGTTTAAGGGTTTGGGTCACTTCAATTTGTGCCTTTTAACTGTGATTAAGTTCAGATGAAGTGTTTATGCTGCTCATGCTATCAATCATGGCAGTGATAGTGTTTTACCTTACAATCTAAATGTTTGGATCCAATCCGGTGCTGTAGAGGTTTGAAGAGTAAGATTATTGTGAGATTCTAACCGGAGGAGAATGAAACATTCTTTATTAAGGTGTGTAAACCTCTCCCTAGCATATGCGTTTTAAAATCTTGAGGGGAAGCCCGGAAGGGAAAGTTCAAAGAGGACAATATATGTTAGTGGTGGGATTGGGCCATTATAAATAGTATTGTCGCCGGACACGAGGCGGTGTGTTAGCAAGGTCGTTGGGCTCCGAAGGGGTGTGGATTAGGGGTCCCACGTCGATTTGAGAAGGAAACAAGTGCAGTGAGGACGCTGGGACCCAAAGAGGGTGGATTGTGAGATCCCACATTGGTTGGGGAGGACGAAACATTCTTTATAAGGGTGTGGAAACCTCTTCCTAGCGAACGTGTTTTAAAAACCTTGAGGAGAAGTTTAGAAGGAAAACCATAAAGAGGACAATATCGGCTAGTAGTGGGTTTAAGGGGAGCCGGTAATTTTTGTAATTTTCTTATCTAAACCTGGAACTATGCCTATAAACACAATGTACTTCATTTTCTATATGCTTGTTTGATTGGGTTTACTTTTATCTTCTTCTGTGTCAGGACAGCCCATTAGCACTTGCCCAGGCTCATGAGACAAGAGACAAGCTCATGGCTTCCCATCCTGAGCTAGCTGAAGATGGTGCCATTCAGATTGTTGTAATCAAAACCACCGGTGACAAAATACTCTCTCAGCCACTTGCAGACATTGGTGGGAAAGGCTTATTTACAAAAGAGATTGATGATGCACTTATCAATGGTGACATAGACATTGCTGTTCACTCAATGAAAGATGTCCCAACTTACTTACCTGAAAAAACCATCCTCCCGTGTAACCTTCCGAGGGAGGACGTCCGGGATGCATTCATTTCTCTACGTGCAGGTTCACTTGCCGAGCTTCCAGCTGGAAGCGTCGTCGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATCGATATCCGTCCCTCGAAGTAAGTATGGTGTGTTATGTAATGGAAAAATATCCAGAAAGTTTATGTTCTACGTTTACATTGCTAACTACCTCGCACTACATTGCTGTTGTGTATGTTGAATTCTGATTCTTGAAGGAATGTTTATGTGTGAGATCCCACATCGGTTGGAGAGGGGAACGAAACATTTCTTATAAGGGTGTGGAAACCTCTTTCTAGTAGAAACGTTTTAAAATCTTGAGAGGAAGCCCTTGAAGGGAAAGCCCAAAGAGGACAGTATCTGCTAGCTGTGGGCTTGGACTGTTACCAATGGTATCAGAGTCAAACACTGGACAGTGTGCTAGAAGGCATCGGTTGGAGAGAGGAACAAAGCATTTTTTATACGGGTGTGGAAACCTCTCACTAGCATACACGTTTTAAAACCGTGAGGTTGATAGCGATATGCAATGGGCCAAAACAGACAATATCTACTAACATGGTAGTGTTTAACGAGTCTTAAATCTTCAATTTTGTATGTTGGTTTTGACACTCGCTCTCGTTTTTTCCATTCTTTGGGTGTCATTTGTTAGGTACTCGAGAATTTTCGAGGTAATGTCCAAACAAGGTTGAAAAAACTAAACGAAGGAGTAGTCCAAGCAACACTATTAGCTCTAGCTGGACTTCGACGTTTAAATATGACAGAAAACGTTACTTCGATCCTTTCAATCGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCTATCGGTATCGCCTGTCGAAGCGACGATGACAAAATGGTGTGTTTTCATTGGCTATACAAATCACTAAATGACATCTTATTTGAGCTGAACTTAGTCATTCCCGTTTGGTTTCTCATTTGCAGGCCAATTACTTAGCCTCATTAAACCATGAGGAAACAAGACTGGCTGTTGTTTGTGAGAGAGCTTTCCTCGAGACTCTTGATGGATCATGCCGAACCCCGATTGCAGGATATGCGTCGAGAGATGAAGATGGCAATTGTATATTCAAAGGGTTGGTAGCTTCCCCAGACGGAACCCGAGGTACGATCTCAACGTTCATGTTCGAATGAAATAACTTCTACATTGCTAGAACATTGAATATCTTCATATTTTATAGTTCTTGAAACTTCTCGACGAGGTTCGTACGCCATCGATGATATGATAGCAATGGGGAAGGATGCTGGGAAGGAGCTCCTTTCTCGAGCAGGTCCGGGTTTTTTTGATAGCTAGTTAGTTTAAAATTTAGGATAAAGATGAAGGCTTCATATCCAAATCTTCAAGTGAGTAGGAGTTATGTTCATCCTTTGTTTTGTTAATTGGTTGTGTAAATTAGAATAGAAATCATTTAGACTATCTTGGGTTCCTCAAATAAGCAATACATTGAAATGGAAAAAGTCTATGCTAATTAGCCATTTCTAATCCTCAAGCTCC

mRNA sequence

ATGAGAATCCTATCTGCCAACGCGAATGCAGACATTTGGCCTGTTGTGTCATCAAAAACAATCCCTTCTAAGCTAACCCATCTCCTTTTTCTTTCTTCTTCTTCTTCTTCTTCTTCGTCTTCTCAAATGGTTGCTTTATCTTCCCCTTCTGTAAGCAACTCTCCCATGGCTCGCCCTTGTAATCTTGCCTCCCTTTCTCTTCCTGGGTTCTCCTCACTTTCCCTTAAACCTCCGGCTTTCACTAATGCCCCCAAGAAATTTCATGGGTTTGGCCTCATAAGAGCCTCTGTCGCTGTGGAGCAGCAGCCAGAGAAGACCAAGGTTGCTCTCCTCAGAATTGGCACCAGAGGAAGCCCATTAGCACTTGCCCAGGCTCATGAGACAAGAGACAAGCTCATGGCTTCCCATCCTGAGCTAGCTGAAGATGGTGCCATTCAGATTGTTGTAATCAAAACCACCGGTGACAAAATACTCTCTCAGCCACTTGCAGACATTGGTGGGAAAGGCTTATTTACAAAAGAGATTGATGATGCACTTATCAATGGTGACATAGACATTGCTGTTCACTCAATGAAAGATGTCCCAACTTACTTACCTGAAAAAACCATCCTCCCGTGTAACCTTCCGAGGGAGGACGTCCGGGATGCATTCATTTCTCTACGTGCAGGTTCACTTGCCGAGCTTCCAGCTGGAAGCGTCGTCGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATCGATATCCGTCCCTCGAAGTACTCGAGAATTTTCGAGGTAATGTCCAAACAAGGTTGAAAAAACTAAACGAAGGAGTAGTCCAAGCAACACTATTAGCTCTAGCTGGACTTCGACGTTTAAATATGACAGAAAACGTTACTTCGATCCTTTCAATCGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCTATCGGTATCGCCTGTCGAAGCGACGATGACAAAATGGCCAATTACTTAGCCTCATTAAACCATGAGGAAACAAGACTGGCTGTTGTTTGTGAGAGAGCTTTCCTCGAGACTCTTGATGGATCATGCCGAACCCCGATTGCAGGATATGCGTCGAGAGATGAAGATGGCAATTGTATATTCAAAGGGTTGGTAGCTTCCCCAGACGGAACCCGAGTTCTTGAAACTTCTCGACGAGGTTCGTACGCCATCGATGATATGATAGCAATGGGGAAGGATGCTGGGAAGGAGCTCCTTTCTCGAGCAGGTCCGGGTTTTTTTGATAGCTAGTTAGTTTAAAATTTAGGATAAAGATGAAGGCTTCATATCCAAATCTTCAAGTGAGTAGGAGTTATGTTCATCCTTTGTTTTGTTAATTGGTTGTGTAAATTAGAATAGAAATCATTTAGACTATCTTGGGTTCCTCAAATAAGCAATACATTGAAATGGAAAAAGTCTATGCTAATTAGCCATTTCTAATCCTCAAGCTCC

Coding sequence (CDS)

ATGAGAATCCTATCTGCCAACGCGAATGCAGACATTTGGCCTGTTGTGTCATCAAAAACAATCCCTTCTAAGCTAACCCATCTCCTTTTTCTTTCTTCTTCTTCTTCTTCTTCTTCGTCTTCTCAAATGGTTGCTTTATCTTCCCCTTCTGTAAGCAACTCTCCCATGGCTCGCCCTTGTAATCTTGCCTCCCTTTCTCTTCCTGGGTTCTCCTCACTTTCCCTTAAACCTCCGGCTTTCACTAATGCCCCCAAGAAATTTCATGGGTTTGGCCTCATAAGAGCCTCTGTCGCTGTGGAGCAGCAGCCAGAGAAGACCAAGGTTGCTCTCCTCAGAATTGGCACCAGAGGAAGCCCATTAGCACTTGCCCAGGCTCATGAGACAAGAGACAAGCTCATGGCTTCCCATCCTGAGCTAGCTGAAGATGGTGCCATTCAGATTGTTGTAATCAAAACCACCGGTGACAAAATACTCTCTCAGCCACTTGCAGACATTGGTGGGAAAGGCTTATTTACAAAAGAGATTGATGATGCACTTATCAATGGTGACATAGACATTGCTGTTCACTCAATGAAAGATGTCCCAACTTACTTACCTGAAAAAACCATCCTCCCGTGTAACCTTCCGAGGGAGGACGTCCGGGATGCATTCATTTCTCTACGTGCAGGTTCACTTGCCGAGCTTCCAGCTGGAAGCGTCGTCGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATCGATATCCGTCCCTCGAAGTACTCGAGAATTTTCGAGGTAATGTCCAAACAAGGTTGAAAAAACTAAACGAAGGAGTAGTCCAAGCAACACTATTAGCTCTAGCTGGACTTCGACGTTTAAATATGACAGAAAACGTTACTTCGATCCTTTCAATCGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCTATCGGTATCGCCTGTCGAAGCGACGATGACAAAATGGCCAATTACTTAGCCTCATTAAACCATGAGGAAACAAGACTGGCTGTTGTTTGTGAGAGAGCTTTCCTCGAGACTCTTGATGGATCATGCCGAACCCCGATTGCAGGATATGCGTCGAGAGATGAAGATGGCAATTGTATATTCAAAGGGTTGGTAGCTTCCCCAGACGGAACCCGAGTTCTTGAAACTTCTCGACGAGGTTCGTACGCCATCGATGATATGATAGCAATGGGGAAGGATGCTGGGAAGGAGCTCCTTTCTCGAGCAGGTCCGGGTTTTTTTGATAGCTAG

Protein sequence

MRILSANANADIWPVVSSKTIPSKLTHLLFLSSSSSSSSSSQMVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQPEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS

Homology

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BLAST of CmaCh14G009760 vs. ExPASy Swiss-Prot
Match: Q43082 (Porphobilinogen deaminase, chloroplastic OS=Pisum sativum OX=3888 GN=HEMC PE=1 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 8.8e-160
Identity = 291/355 (81.97%), Postives = 321/355 (90.42%), Query Frame = 0


Query: 66  SLPGFSSLSLKPPAFTNAPKKFHGFG--LIRASVAVEQQPEKTKVALLRIGTRGSPLALA 125
           S P   SLSL   +F  +  K   F    IRAS+AVEQQ ++ K AL+RIGTRGSPLALA
Sbjct: 15  SAPSNPSLSLFTSSFRFSSFKTSPFSKCRIRASLAVEQQTQQNKTALIRIGTRGSPLALA 74

Query: 126 QAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGD 185
           QAHETRDKLMASH ELAE+GAIQIV+IKTTGDKILSQPLADIGGKGLFTKEID+ALINGD
Sbjct: 75  QAHETRDKLMASHTELAEEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDEALINGD 134

Query: 186 IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKS 245
           IDIAVHSMKDVPTYLPE+TILPCNLPREDVRDAFISL A SLA+LPAGSV+GTASLRRKS
Sbjct: 135 IDIAVHSMKDVPTYLPEETILPCNLPREDVRDAFISLSAASLADLPAGSVIGTASLRRKS 194

Query: 246 QLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEML 305
           Q+L+RYPSL V +NFRGNVQTRL+KL+EGVV+ATLLALAGL+RLNMTENVTS LSID+ML
Sbjct: 195 QILHRYPSLTVQDNFRGNVQTRLRKLSEGVVKATLLALAGLKRLNMTENVTSTLSIDDML 254

Query: 306 PAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRD 365
           PAVAQGAIGIACRS+DDKMA YLASLNHEETRLA+ CERAFL TLDGSCRTPIAGYASRD
Sbjct: 255 PAVAQGAIGIACRSNDDKMAEYLASLNHEETRLAISCERAFLTTLDGSCRTPIAGYASRD 314

Query: 366 EDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 419
           +DGNC+F+GLVASPDGTRVLETSR GSY  +DM+ +GKDAG+ELLSRAGPGFF+S
Sbjct: 315 KDGNCLFRGLVASPDGTRVLETSRIGSYTYEDMMKIGKDAGEELLSRAGPGFFNS 369

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BLAST of CmaCh14G009760 vs. ExPASy Swiss-Prot
Match: Q43316 (Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMC PE=1 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 8.5e-155
Identity = 287/384 (74.74%), Postives = 322/384 (83.85%), Query Frame = 0


Query: 33  SSSSSSSSSQMVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGL 92
           +SSS S + ++V    P    S     C+L S+S  GFS   +  PA     +K    G 
Sbjct: 4   ASSSLSQAHKVVLTRQP----SSRVNTCSLGSVSAIGFSLPQISSPALGKCRRKQSSSGF 63

Query: 93  IRASVAVEQQPEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKT 152
           ++A VAVEQ   KT+ A++RIGTRGSPLALAQA+ETR+KL   HPEL EDGAI I +IKT
Sbjct: 64  VKACVAVEQ---KTRTAIIRIGTRGSPLALAQAYETREKLKKKHPELVEDGAIHIEIIKT 123

Query: 153 TGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPRED 212
           TGDKILSQPLADIGGKGLFTKEID+ALING IDIAVHSMKDVPTYLPEKTILPCNLPRED
Sbjct: 124 TGDKILSQPLADIGGKGLFTKEIDEALINGHIDIAVHSMKDVPTYLPEKTILPCNLPRED 183

Query: 213 VRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEG 272
           VRDAFI L A +LAELPAGSVVGTASLRRKSQ+L++YP+L V ENFRGNVQTRL KL  G
Sbjct: 184 VRDAFICLTAATLAELPAGSVVGTASLRRKSQILHKYPALHVEENFRGNVQTRLSKLQGG 243

Query: 273 VVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHE 332
            VQATLLALAGL+RL+MTENV SILS+DEMLPAVAQGAIGIACR+DDDKMA YLASLNHE
Sbjct: 244 KVQATLLALAGLKRLSMTENVASILSLDEMLPAVAQGAIGIACRTDDDKMATYLASLNHE 303

Query: 333 ETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYA 392
           ETRLA+ CERAFLETLDGSCRTPIAGYAS+DE+GNCIF+GLVASPDGT+VLETSR+G Y 
Sbjct: 304 ETRLAISCERAFLETLDGSCRTPIAGYASKDEEGNCIFRGLVASPDGTKVLETSRKGPYV 363

Query: 393 IDDMIAMGKDAGKELLSRAGPGFF 417
            +DM+ MGKDAG+ELLSRAGPGFF
Sbjct: 364 YEDMVKMGKDAGQELLSRAGPGFF 380

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BLAST of CmaCh14G009760 vs. ExPASy Swiss-Prot
Match: Q6H6D2 (Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=HEMC PE=2 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 1.7e-139
Identity = 251/326 (76.99%), Postives = 285/326 (87.42%), Query Frame = 0


Query: 93  IRASVAVEQQPEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKT 152
           +RA+VAV+ + +  KV+L+RIGTRGSPLALAQAHETRDKL A+H ELAE+GA++IV+IKT
Sbjct: 33  VRAAVAVQAEAQ-AKVSLIRIGTRGSPLALAQAHETRDKLKAAHSELAEEGAVEIVIIKT 92

Query: 153 TGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPRED 212
           TGD IL +PLADIGGKGLFTKEIDDAL+ G IDIAVHSMKDVPTYLPE TILPCNLPRED
Sbjct: 93  TGDMILDKPLADIGGKGLFTKEIDDALLQGRIDIAVHSMKDVPTYLPEGTILPCNLPRED 152

Query: 213 VRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEG 272
           VRDAFI L A SLAELPAGSVVG+ASLRR+SQ+L +YPSL+V+ NFRGNVQTRL+KL EG
Sbjct: 153 VRDAFICLTASSLAELPAGSVVGSASLRRQSQILYKYPSLKVV-NFRGNVQTRLRKLKEG 212

Query: 273 VVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHE 332
            V ATLLALAGL+RLNM E  TS+LS+DEMLPAVAQGAIGIACRS DD M NYL+SLNHE
Sbjct: 213 DVHATLLALAGLKRLNMAETATSVLSVDEMLPAVAQGAIGIACRSSDDTMMNYLSSLNHE 272

Query: 333 ETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYA 392
           +TRLAV CER FL  LDG+CRTPIA YASRD+DGNC F+GL+ASPDG+ V ETSR G Y 
Sbjct: 273 DTRLAVACEREFLSVLDGNCRTPIAAYASRDKDGNCSFRGLLASPDGSTVYETSRTGPYD 332

Query: 393 IDDMIAMGKDAGKELLSRAGPGFFDS 419
            D M+ MGKDAG EL ++AGPGFFDS
Sbjct: 333 FDIMVEMGKDAGHELKAKAGPGFFDS 356

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BLAST of CmaCh14G009760 vs. ExPASy Swiss-Prot
Match: A1B853 (Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) OX=318586 GN=hemC PE=3 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 1.4e-85
Identity = 175/307 (57.00%), Postives = 221/307 (71.99%), Query Frame = 0


Query: 111 LRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGL 170
           +RIGTRGS LALAQAHETRD+LMA+H  LA D A +IVVIKTTGD++L +PL +IGGKGL
Sbjct: 11  IRIGTRGSALALAQAHETRDRLMAAH-GLAAD-AFRIVVIKTTGDRVLDRPLKEIGGKGL 70

Query: 171 FTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRAGSLAELPA 230
           FT+EI+DAL+  +IDIAVHSMKD+PT  PE  ++ C LPREDVRDAF+S +  +++ELP 
Sbjct: 71  FTREIEDALLAHEIDIAVHSMKDMPTIQPEGLVIDCYLPREDVRDAFVSAQFAAISELPQ 130

Query: 231 GSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALAGLRRLNMT 290
           G+VVG++SLRR++QL  R P L+++E FRGNVQTRLKKL +GV  AT LA+AGL RL M 
Sbjct: 131 GAVVGSSSLRRRAQLAARRPDLKLVE-FRGNVQTRLKKLEDGVAVATFLAMAGLTRLGML 190

Query: 291 ENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCERAFLETLDG 350
                 +  DEMLPAVAQG IG+  R+DD + A+ LA+++  ++ L V  ERAFL  LDG
Sbjct: 191 HVARGAVEPDEMLPAVAQGCIGVERRADDARTASLLAAISDRDSALRVTAERAFLARLDG 250

Query: 351 SCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKDAGKELLSR 410
           SC+TPIAG A    D     +G +  PDG+ V+   R G  A  D  AMG D  +EL  R
Sbjct: 251 SCQTPIAGLAELQGD-RLRLRGEILRPDGSEVIAAERVGPAA--DGAAMGTDLAEELRGR 310

Query: 411 AGPGFFD 418
           A   FFD
Sbjct: 311 APADFFD 311

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BLAST of CmaCh14G009760 vs. ExPASy Swiss-Prot
Match: Q3SVF3 (Porphobilinogen deaminase OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255) OX=323098 GN=hemC PE=3 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.2e-84
Identity = 174/316 (55.06%), Postives = 217/316 (68.67%), Query Frame = 0


Query: 101 QQPEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQ 160
           Q  ++T + L  IGTRGSPLALAQAHE RD+L  +H    E   I I  I+T+GD I  +
Sbjct: 2   QSSDETDI-LATIGTRGSPLALAQAHEVRDRLARAHQVAPE--RIAIKTIRTSGDAIQDR 61

Query: 161 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 220
           PL D+GGKGLFTKEI++AL+ G ID AVHS KDVPT+LP+ T LP  LPREDVRD FIS 
Sbjct: 62  PLFDVGGKGLFTKEIEEALLAGTIDFAVHSSKDVPTFLPDATWLPAFLPREDVRDVFISP 121

Query: 221 RAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLA 280
            AGSL +LPAG+ VGTASLRR++ +L   P L+V  + RGNV+TRL+K++ G   ATLLA
Sbjct: 122 HAGSLNDLPAGATVGTASLRRQAMVLKLRPDLKV-NSLRGNVETRLRKISVGEADATLLA 181

Query: 281 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVC 340
           LAGL RL + +  T IL  DE LPAV QGAI I  R DDD++  ++ ++   ET +A+  
Sbjct: 182 LAGLNRLGLQDKATRILETDEFLPAVGQGAIAIESRRDDDRINAFVKAIGDPETEVALSA 241

Query: 341 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMG 400
           ER+FL  LDGSCRTPI G+   + D    F+GL+ SPDGT   ET+R G+ A  D  A+G
Sbjct: 242 ERSFLALLDGSCRTPIGGHCRVNGD-RIDFRGLIISPDGTEFYETTREGARA--DAAALG 301

Query: 401 KDAGKELLSRAGPGFF 417
            DA  EL  RAG  FF
Sbjct: 302 ADAAHELRERAGEKFF 310

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BLAST of CmaCh14G009760 vs. ExPASy TrEMBL
Match: A0A6J1IZW2 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111481450 PE=3 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 1.7e-206
Identity = 376/376 (100.00%), Postives = 376/376 (100.00%), Query Frame = 0


Query: 43  MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 102
           MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ
Sbjct: 1   MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 60

Query: 103 PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 162
           PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 163 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 222
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 223 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 282
           GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 283 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 342
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 343 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 402
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 403 AGKELLSRAGPGFFDS 419
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of CmaCh14G009760 vs. ExPASy TrEMBL
Match: A0A6J1F354 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111441758 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 9.6e-202
Identity = 369/376 (98.14%), Postives = 370/376 (98.40%), Query Frame = 0


Query: 43  MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 102
           MVALSSPSVS+SPMA PCNLASLSLPGFSSLSLKPPAFTN PKK HGFGLIRASVAVEQQ
Sbjct: 1   MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 60

Query: 103 PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 162
             KTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 163 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 222
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 223 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 282
           GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 283 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 342
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMA YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 300

Query: 343 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 402
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 403 AGKELLSRAGPGFFDS 419
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of CmaCh14G009760 vs. ExPASy TrEMBL
Match: A0A6J1GCC7 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111452689 PE=3 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.8e-192
Identity = 351/376 (93.35%), Postives = 359/376 (95.48%), Query Frame = 0


Query: 43  MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 102
           M   SSPS+S+ PMARPCNL SLS  GFSSLSLKPPAF+   KKFHGFGLIRASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQ 60

Query: 103 PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 162
            EKTKVAL+RIGTRGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 163 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 222
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL  
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 223 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 282
           GSLAELPAGSV+GTASLRRKSQLLNRYPSL VLENFRGNVQTRL+KLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 283 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 342
           GLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 343 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 402
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAIDDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKD 360

Query: 403 AGKELLSRAGPGFFDS 419
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of CmaCh14G009760 vs. ExPASy TrEMBL
Match: A0A6J1KA97 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111493083 PE=3 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 2.6e-191
Identity = 348/376 (92.55%), Postives = 358/376 (95.21%), Query Frame = 0


Query: 43  MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 102
           M   SSPS+S+ PMARPCNL SLS  GFSSLSLKPPAF+   K+FHG GLIRASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGDKRFHGVGLIRASVAVEQQ 60

Query: 103 PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 162
            EKTKVAL+RIGTRGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPL
Sbjct: 61  AEKTKVALVRIGTRGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPL 120

Query: 163 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 222
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL  
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSV 180

Query: 223 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 282
           GSLAELPAGSV+GTASLRRKSQLLNRYPSL VLENFRGNVQTRL+KLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 283 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 342
           GLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 343 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 402
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAIDDM+AMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMVAMGKD 360

Query: 403 AGKELLSRAGPGFFDS 419
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of CmaCh14G009760 vs. ExPASy TrEMBL
Match: A0A6J1DII9 (Hydroxymethylbilane synthase OS=Momordica charantia OX=3673 GN=LOC111020357 PE=3 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 9.3e-189
Identity = 342/376 (90.96%), Postives = 359/376 (95.48%), Query Frame = 0


Query: 43  MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 102
           M   SSP +++SPMARPCN+ SLS  GFSSLSLKPPAF N  KKF G GL+RASVAVEQQ
Sbjct: 1   MGVFSSPPITHSPMARPCNVGSLSPLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60

Query: 103 PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 162
            EKTKVAL+RIGTRGSPLALAQA+ETRDKLMA HPELAEDGAIQI++IKTTGDKILSQPL
Sbjct: 61  TEKTKVALVRIGTRGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPL 120

Query: 163 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 222
           ADIGGKGLFTKEIDDALIN DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL A
Sbjct: 121 ADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA 180

Query: 223 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 282
           GSLAELPAGSV+GTASLRRKSQLLNRYPSL+VLENFRGNVQTRL+KLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALA 240

Query: 283 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 342
           GLRRLNMTENVTS+LSIDEMLPAVAQGAIGIACRS+DDKMANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCER 300

Query: 343 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 402
           AFLE LDGSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLETSRRG+YAIDDM+AMGKD
Sbjct: 301 AFLEMLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKD 360

Query: 403 AGKELLSRAGPGFFDS 419
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of CmaCh14G009760 vs. NCBI nr
Match: KAG6581556.1 (Porphobilinogen deaminase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 769.2 bits (1985), Expect = 1.8e-218
Identity = 405/418 (96.89%), Postives = 408/418 (97.61%), Query Frame = 0


Query: 1   MRILSANANADIWPVVSSKTIPSKLTHLLFLSSSSSSSSSSQMVALSSPSVSNSPMARPC 60
           MRILSANANA+IWPV SSKTIPSKLTHLLFL   SSSSSSSQMVALSSPSVS+SPMA PC
Sbjct: 1   MRILSANANAEIWPVGSSKTIPSKLTHLLFL---SSSSSSSQMVALSSPSVSHSPMAHPC 60

Query: 61  NLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQPEKTKVALLRIGTRGSPL 120
           NLASLSLPGFSSLSLKPPAFTN PKK HGFGLIRASVAVEQQ  KTKVALLRIGTRGSPL
Sbjct: 61  NLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQAGKTKVALLRIGTRGSPL 120

Query: 121 ALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALI 180
           ALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALI
Sbjct: 121 ALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALI 180

Query: 181 NGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLR 240
           NGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLR
Sbjct: 181 NGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLR 240

Query: 241 RKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALAGLRRLNMTENVTSILSID 300
           RKSQLLNRYPSLEVLENFRGNVQTRLKKLN+GVVQATLLALAGLRRLNMTENVTSILSID
Sbjct: 241 RKSQLLNRYPSLEVLENFRGNVQTRLKKLNKGVVQATLLALAGLRRLNMTENVTSILSID 300

Query: 301 EMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYA 360
           EMLPAVAQGAIGIACRSDDDKMA YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYA
Sbjct: 301 EMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYA 360

Query: 361 SRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 419
           SRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS
Sbjct: 361 SRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 415

[Close]

BLAST of CmaCh14G009760 vs. NCBI nr
Match: XP_022982636.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 728.4 bits (1879), Expect = 3.5e-206
Identity = 376/376 (100.00%), Postives = 376/376 (100.00%), Query Frame = 0


Query: 43  MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 102
           MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ
Sbjct: 1   MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 60

Query: 103 PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 162
           PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 163 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 222
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 223 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 282
           GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 283 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 342
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 300

Query: 343 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 402
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 403 AGKELLSRAGPGFFDS 419
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of CmaCh14G009760 vs. NCBI nr
Match: XP_022934624.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 712.6 bits (1838), Expect = 2.0e-201
Identity = 369/376 (98.14%), Postives = 370/376 (98.40%), Query Frame = 0


Query: 43  MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 102
           MVALSSPSVS+SPMA PCNLASLSLPGFSSLSLKPPAFTN PKK HGFGLIRASVAVEQQ
Sbjct: 1   MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 60

Query: 103 PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 162
             KTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 163 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 222
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 223 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 282
           GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 283 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 342
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMA YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 300

Query: 343 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 402
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 403 AGKELLSRAGPGFFDS 419
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of CmaCh14G009760 vs. NCBI nr
Match: KGN58748.2 (hypothetical protein Csa_002701 [Cucumis sativus])

HSP 1 Score: 711.8 bits (1836), Expect = 3.4e-201
Identity = 381/432 (88.19%), Postives = 392/432 (90.74%), Query Frame = 0


Query: 1   MRILSANANADIWPVVSSKTIPSKLTH--------------LLFLSSSSSSSSSSQMVAL 60
           MRILSANANADI PV SSKTIPSKLTH              LL L S +   + SQM  L
Sbjct: 1   MRILSANANADICPVGSSKTIPSKLTHFPFLLSFFLLLPLLLLLLISLNLKQTLSQMGVL 60

Query: 61  SSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQPEKT 120
           SSPSVS+SPM RPCN+ SLS  GFSSLSLKPP F+N  KKFHG GLIRA VA EQQ EKT
Sbjct: 61  SSPSVSHSPMPRPCNVGSLSFLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQVEKT 120

Query: 121 KVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIG 180
           KVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIG
Sbjct: 121 KVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIG 180

Query: 181 GKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRAGSLA 240
           GKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL AGS A
Sbjct: 181 GKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFA 240

Query: 241 ELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALAGLRR 300
           ELPAGS++GTASLRRKSQLLNRYPSL+VLENFRGNVQTRL+KLNEGVVQATLLALAGLRR
Sbjct: 241 ELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRR 300

Query: 301 LNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCERAFLE 360
           LNMTENVTSILSIDEMLPAVAQGAIGIACRSDDD MANYLASLNHEETRLAVVCERAFLE
Sbjct: 301 LNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLE 360

Query: 361 TLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKDAGKE 419
           TLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAI+DMIAMGKDAG+E
Sbjct: 361 TLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQE 420

[Close]

BLAST of CmaCh14G009760 vs. NCBI nr
Match: XP_023529085.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 711.4 bits (1835), Expect = 4.4e-201
Identity = 369/376 (98.14%), Postives = 370/376 (98.40%), Query Frame = 0


Query: 43  MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 102
           MVALSSPSVS+SPMARPCNLASLSLPGFSSLSLKPPAF NAPKK HG GLIRASVAVEQQ
Sbjct: 1   MVALSSPSVSHSPMARPCNLASLSLPGFSSLSLKPPAFINAPKKLHGLGLIRASVAVEQQ 60

Query: 103 PEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 162
             KTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL
Sbjct: 61  AGKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPL 120

Query: 163 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 222
           ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA
Sbjct: 121 ADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRA 180

Query: 223 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 282
           GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA
Sbjct: 181 GSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALA 240

Query: 283 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCER 342
           GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMA YLASLNHEETRLAVVCER
Sbjct: 241 GLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCER 300

Query: 343 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 402
           AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD
Sbjct: 301 AFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKD 360

Query: 403 AGKELLSRAGPGFFDS 419
           AGKELLSRAGPGFFDS
Sbjct: 361 AGKELLSRAGPGFFDS 376

[Close]

BLAST of CmaCh14G009760 vs. TAIR 10
Match: AT5G08280.1 (hydroxymethylbilane synthase )

HSP 1 Score: 548.1 bits (1411), Expect = 6.0e-156
Identity = 287/384 (74.74%), Postives = 322/384 (83.85%), Query Frame = 0


Query: 33  SSSSSSSSSQMVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGL 92
           +SSS S + ++V    P    S     C+L S+S  GFS   +  PA     +K    G 
Sbjct: 4   ASSSLSQAHKVVLTRQP----SSRVNTCSLGSVSAIGFSLPQISSPALGKCRRKQSSSGF 63

Query: 93  IRASVAVEQQPEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKT 152
           ++A VAVEQ   KT+ A++RIGTRGSPLALAQA+ETR+KL   HPEL EDGAI I +IKT
Sbjct: 64  VKACVAVEQ---KTRTAIIRIGTRGSPLALAQAYETREKLKKKHPELVEDGAIHIEIIKT 123

Query: 153 TGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPRED 212
           TGDKILSQPLADIGGKGLFTKEID+ALING IDIAVHSMKDVPTYLPEKTILPCNLPRED
Sbjct: 124 TGDKILSQPLADIGGKGLFTKEIDEALINGHIDIAVHSMKDVPTYLPEKTILPCNLPRED 183

Query: 213 VRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEG 272
           VRDAFI L A +LAELPAGSVVGTASLRRKSQ+L++YP+L V ENFRGNVQTRL KL  G
Sbjct: 184 VRDAFICLTAATLAELPAGSVVGTASLRRKSQILHKYPALHVEENFRGNVQTRLSKLQGG 243

Query: 273 VVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHE 332
            VQATLLALAGL+RL+MTENV SILS+DEMLPAVAQGAIGIACR+DDDKMA YLASLNHE
Sbjct: 244 KVQATLLALAGLKRLSMTENVASILSLDEMLPAVAQGAIGIACRTDDDKMATYLASLNHE 303

Query: 333 ETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYA 392
           ETRLA+ CERAFLETLDGSCRTPIAGYAS+DE+GNCIF+GLVASPDGT+VLETSR+G Y 
Sbjct: 304 ETRLAISCERAFLETLDGSCRTPIAGYASKDEEGNCIFRGLVASPDGTKVLETSRKGPYV 363

Query: 393 IDDMIAMGKDAGKELLSRAGPGFF 417
            +DM+ MGKDAG+ELLSRAGPGFF
Sbjct: 364 YEDMVKMGKDAGQELLSRAGPGFF 380

The following BLAST results are available for this feature:

BLAST of CmaCh14G009760 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43082	8.8e-160	81.97	Porphobilinogen deaminase, chloroplastic OS=Pisum sativum OX=3888 GN=HEMC PE=1 S...	[more]
Q43316	8.5e-155	74.74	Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMC...	[more]
Q6H6D2	1.7e-139	76.99	Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica OX=3994...	[more]
A1B853	1.4e-85	57.00	Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) OX=318586...	[more]
Q3SVF3	1.2e-84	55.06	Porphobilinogen deaminase OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 1...	[more]

BLAST of CmaCh14G009760 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IZW2	1.7e-206	100.00	Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111481450 PE=3 SV...	[more]
A0A6J1F354	9.6e-202	98.14	Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111441758 PE=3 ...	[more]
A0A6J1GCC7	1.8e-192	93.35	Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111452689 PE=3 ...	[more]
A0A6J1KA97	2.6e-191	92.55	Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111493083 PE=3 SV...	[more]
A0A6J1DII9	9.3e-189	90.96	Hydroxymethylbilane synthase OS=Momordica charantia OX=3673 GN=LOC111020357 PE=3...	[more]

BLAST of CmaCh14G009760 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6581556.1	1.8e-218	96.89	Porphobilinogen deaminase, chloroplastic, partial [Cucurbita argyrosperma subsp....	[more]
XP_022982636.1	3.5e-206	100.00	porphobilinogen deaminase, chloroplastic-like [Cucurbita maxima]	[more]
XP_022934624.1	2.0e-201	98.14	porphobilinogen deaminase, chloroplastic-like [Cucurbita moschata]	[more]
KGN58748.2	3.4e-201	88.19	hypothetical protein Csa_002701 [Cucumis sativus]	[more]
XP_023529085.1	4.4e-201	98.14	porphobilinogen deaminase, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmaCh14G009760 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08280.1	6.0e-156	74.74	hydroxymethylbilane synthase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000860	Porphobilinogen deaminase	PRINTS	PR00151	PORPHBDMNASE	coord: 253..270 score: 52.38 coord: 340..357 score: 53.17 coord: 233..250 score: 57.41 coord: 185..204 score: 61.19 coord: 154..174 score: 66.21
IPR000860	Porphobilinogen deaminase	TIGRFAM	TIGR00212	TIGR00212	coord: 111..409 e-value: 6.7E-101 score: 335.1
IPR000860	Porphobilinogen deaminase	PANTHER	PTHR11557	PORPHOBILINOGEN DEAMINASE	coord: 54..417
IPR000860	Porphobilinogen deaminase	HAMAP	MF_00260	Porphobil_deam	coord: 110..410 score: 34.359402
IPR022417	Porphobilinogen deaminase, N-terminal	PFAM	PF01379	Porphobil_deam	coord: 111..323 e-value: 1.9E-76 score: 256.2
None	No IPR available	GENE3D	3.40.190.10		coord: 212..312 e-value: 5.3E-82 score: 276.1
None	No IPR available	GENE3D	3.40.190.10		coord: 111..320 e-value: 5.3E-82 score: 276.1
None	No IPR available	PANTHER	PTHR11557:SF8	BNAC02G01240D PROTEIN	coord: 54..417
None	No IPR available	CDD	cd13648	PBP2_PBGD_1	coord: 110..389 e-value: 0.0 score: 516.58
None	No IPR available	SUPERFAMILY	53850	Periplasmic binding protein-like II	coord: 111..328
IPR022418	Porphobilinogen deaminase, C-terminal	PFAM	PF03900	Porphobil_deamC	coord: 336..408 e-value: 2.7E-15 score: 56.5
IPR036803	Porphobilinogen deaminase, C-terminal domain superfamily	GENE3D	3.30.160.40		coord: 331..417 e-value: 4.9E-24 score: 86.5
IPR036803	Porphobilinogen deaminase, C-terminal domain superfamily	SUPERFAMILY	54782	Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain	coord: 330..417
IPR022419	Porphobilinogen deaminase, dipyrromethane cofactor binding site	PROSITE	PS00533	PORPHOBILINOGEN_DEAM	coord: 341..357

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh14G009760.1	CmaCh14G009760.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018160	peptidyl-pyrromethane cofactor linkage
biological_process	GO:0006782	protoporphyrinogen IX biosynthetic process
biological_process	GO:0050790	regulation of catalytic activity
biological_process	GO:0017157	regulation of exocytosis
biological_process	GO:0033014	tetrapyrrole biosynthetic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005096	GTPase activator activity
molecular_function	GO:0004418	hydroxymethylbilane synthase activity
molecular_function	GO:0045159	myosin II binding
molecular_function	GO:0019905	syntaxin binding