CmaCh14G009140 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh14G009140
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	pectinesterase inhibitor-like
Location	Cma_Chr14: 4742703 .. 4744713 (+)
RNA-Seq Expression	CmaCh14G009140
Synteny	CmaCh14G009140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATTTATCTCAGCCATTGTGGTAATTCTGTGCCTGTGCATGGCGACAAAGCGCCACGACGCCACCCGCCCCGTCGAGGAAGGGATGACCCTAATCGAGGAAATGTGTAGCCAAACCAAACACAAGGAGCTGTGCGTGGACTCCCTGACCTCGGACCCCAACAGCTACCCGGCCGACAAGACGGGGCTAGCCCTGGTGGCCCTCCGCTTCGCCTCTTCCAACGCCTCGGACATCTCGGAGAGCATCAAGGTGATGTTCAATGACTCGACCCTGTCCAAGCCGATGGTGCAGGAGGCTCTCCTCGACTGCCTTGACGAGTACCTAGACGCGTCCCAACAGCTGGACGACTCCATTGCAGCGCTGACGGTGAAGGCGTATGGGGACCTGGAGAAATGGGTGAATATAGCCATGACGGATGTGCGCACGTGTGGGACGGAGTTGGGCAGCGAGCGCCCACCGCTGCTGACGGAGAGGAATGATGTGTTTATTAAGCTGTGTGAAATTGCGCTGACCATTTCCAAGATTGCGCAGGGTGAAAAGAACTGATTATAATTATTATTATTATTCTATTTCTTGTACGGAGGACCCAAATCGCTGGCTAAGCCATTTTTATTTGTGGTTAAGCTTAGAGGGAGATATTGGGGTTTGGATAAAAACACAAAACTTTAATTACTTTTGAAACATAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATAATGTTTTGTGGGTAATGGCCATCTCATGTTATTATGCCTTTTGGGTCTTTTTCTAATAAAAATCTATATGTTTCATCAAATTCAACCTTTCCATTTATACCTTATGCTTAAACCAAAATAACCTAATTGATTTGTTCAGATATTCACGACCAAGAAACATTAAATTCTCTTTTGGATCCAGTTTGGATATGGAAGGCCCGGAAAAGAGGAGGAAAGACTAATGCCAATAAACCTCTATTATTAAATTCAGAGGAGGAAAGACTAATGCCAATAAACGGGTGAATTCAGCCTGATAGTACTCTATTTTATTATTGTACCCTGATAGTACTCTATTTTATTATTGTACCCTGATAGTACTCTATTTTATTATTGTACGCTATCCAGTGTAATTCCAGTGTAATAGTACCCTATTTATCCAATCCAATGTAATAGTACCCTATTTTTAGAACGTCTAACTATTTAATATAATTTATCTAATGGGTTATACACATATCCACAAAATTGGTATGACATTAGTGAAAAAGTTAGTATATTCATCAAAATGGTGATCTCGGTTGACTGGTGTATATAGAATCATAGAGGGAGATGATCTATAAAGTATAGTTGATTCGTGTATATAGAATAAATGTTTTCATCTATATATTTAATGAAAATTTTGAAGGTAGTAGGTTTGTCTAAGACTTAAGATCTTCGGCACGTAAATGCGGCATTTTATGCTTTATAAAGTATAGTTGAAGAATTTGAGACATGTAAAAATATTAATGAAAGTTTTGAAATGAGGCCTCAAAATTTTAATCACAGGTATAGATTAAAAAGGTGTGAGGATATTTTTATATTAACTTTTCCATACCTTTAAAAGATATTATATTTAAAATTGTTTAAGCCCATTAACAAAGAAGTAAGTGATAAAAATAAATTAATTGGAAAAGGAAAAATAAGTAAGGAAAAAAACACAAAATAATAAAATCATCATCGAACGGCATTTGTGTGGTCTTGGCTGAACTCATCATCTCTCTCTCCTTCTCTCTCTCTTCTTCCCCTCACTGTTATCTCGTGGCCTTCTTCGCTTCAATCCCCTTTGCTTAATTCTCTATCACCGATGCGTGACGATTCACAGCTCCGACACCCTCTTCTTCTGATTCCTCTGCGTTGCGATTTCTTTTTGTTATTGAGATTTCGCCAGTGGCCGCCTCGATTCTGAGTTTCTGTTCCGCTTTCCTTCCACCACGGGAGTGGTTGTTTAATTTAATGCTAGTTGGATTCAATGAGATTTTCCTCTCCCACTAG

mRNA sequence

ATGAAATTTATCTCAGCCATTGTGGTAATTCTGTGCCTGTGCATGGCGACAAAGCGCCACGACGCCACCCGCCCCGTCGAGGAAGGGATGACCCTAATCGAGGAAATGTGTAGCCAAACCAAACACAAGGAGCTGTGCGTGGACTCCCTGACCTCGGACCCCAACAGCTACCCGGCCGACAAGACGGGGCTAGCCCTGGTGGCCCTCCGCTTCGCCTCTTCCAACGCCTCGGACATCTCGGAGAGCATCAAGGTGATGTTCAATGACTCGACCCTGTCCAAGCCGATGGTGCAGGAGGCTCTCCTCGACTGCCTTGACGAGTACCTAGACGCGTCCCAACAGCTGGACGACTCCATTGCAGCGCTGACGGTGAAGGCGTATGGGGACCTGGAGAAATGGGTGAATATAGCCATGACGGATGTGCGCACGTGTGGGACGGAGTTGGGCAGCGAGCGCCCACCGCTGCTGACGGAGAGGAATGATGTGTTTATTAAGCTGTGTGAAATTGCGCTGACCATTTCCAAGATTGCGCAGGCTCCGACACCCTCTTCTTCTGATTCCTCTGCGTTGCGATTTCTTTTTGTTATTGAGATTTCGCCAGTGGCCGCCTCGATTCTGAGTTTCTGTTCCGCTTTCCTTCCACCACGGGAGTGGTTGTTTAATTTAATGCTAGTTGGATTCAATGAGATTTTCCTCTCCCACTAG

Coding sequence (CDS)

ATGAAATTTATCTCAGCCATTGTGGTAATTCTGTGCCTGTGCATGGCGACAAAGCGCCACGACGCCACCCGCCCCGTCGAGGAAGGGATGACCCTAATCGAGGAAATGTGTAGCCAAACCAAACACAAGGAGCTGTGCGTGGACTCCCTGACCTCGGACCCCAACAGCTACCCGGCCGACAAGACGGGGCTAGCCCTGGTGGCCCTCCGCTTCGCCTCTTCCAACGCCTCGGACATCTCGGAGAGCATCAAGGTGATGTTCAATGACTCGACCCTGTCCAAGCCGATGGTGCAGGAGGCTCTCCTCGACTGCCTTGACGAGTACCTAGACGCGTCCCAACAGCTGGACGACTCCATTGCAGCGCTGACGGTGAAGGCGTATGGGGACCTGGAGAAATGGGTGAATATAGCCATGACGGATGTGCGCACGTGTGGGACGGAGTTGGGCAGCGAGCGCCCACCGCTGCTGACGGAGAGGAATGATGTGTTTATTAAGCTGTGTGAAATTGCGCTGACCATTTCCAAGATTGCGCAGGCTCCGACACCCTCTTCTTCTGATTCCTCTGCGTTGCGATTTCTTTTTGTTATTGAGATTTCGCCAGTGGCCGCCTCGATTCTGAGTTTCTGTTCCGCTTTCCTTCCACCACGGGAGTGGTTGTTTAATTTAATGCTAGTTGGATTCAATGAGATTTTCCTCTCCCACTAG

Protein sequence

MKFISAIVVILCLCMATKRHDATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIAALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQAPTPSSSDSSALRFLFVIEISPVAASILSFCSAFLPPREWLFNLMLVGFNEIFLSH

Homology

[Close]

BLAST of CmaCh14G009140 vs. ExPASy Swiss-Prot
Match: Q0J8J8 (Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.9e-07
Identity = 42/171 (24.56%), Postives = 77/171 (45.03%), Query Frame = 0


Query: 28  EGMTLIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLALVALRFASSNASDISESIKVMF 87
           +   L+   C+ T + ++CV +L +DP+S  AD  GL+ +A+  A++NAS  +++   + 
Sbjct: 57  QAAALVRATCNSTAYYDVCVSALAADPSSTTADVRGLSAIAVSVAAANASGAAQAAAALA 116

Query: 88  ND------STLSKPMVQEALLDCLDEYLDASQQLDDSIAALTVKAYGDLEKWVNIAMTDV 147
           N       +      VQ  L  C  +Y DA   L  +  ++  + Y      V+      
Sbjct: 117 NGTAPLAAAAAGDGTVQALLRACAGKYGDARDALAAAKESMGQQDYDLATVHVSAGAEYP 176

Query: 148 RTCGTELGSERPPL----LTERNDVFIKLCEIALTISKIAQAPTPSSSDSS 189
           + C      +RP      L  R +   KLC +AL I  +  +   S++++S
Sbjct: 177 QVCKALFRRQRPGAYPAELAAREEALNKLCSVALDIIALLTSSPASNNNNS 227

[Close]

BLAST of CmaCh14G009140 vs. ExPASy TrEMBL
Match: A0A6J1J9E7 (pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111482494 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 2.0e-92
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 0


Query: 1   MKFISAIVVILCLCMATKRHDATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPAD 60
           MKFISAIVVILCLCMATKRHDATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPAD
Sbjct: 1   MKFISAIVVILCLCMATKRHDATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPAD 60

Query: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120
           KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA
Sbjct: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120

Query: 121 ALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ 179
           ALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ
Sbjct: 121 ALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ 178

[Close]

BLAST of CmaCh14G009140 vs. ExPASy TrEMBL
Match: A0A6J1F7F2 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111441544 PE=4 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 4.3e-90
Identity = 172/178 (96.63%), Postives = 176/178 (98.88%), Query Frame = 0


Query: 1   MKFISAIVVILCLCMATKRHDATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPAD 60
           MKF+SAIVV+LCLCMA KRHDATRPVEEGM LIEEMCSQTKHKELCVDSLTSDPNSYPAD
Sbjct: 1   MKFVSAIVVVLCLCMAAKRHDATRPVEEGMALIEEMCSQTKHKELCVDSLTSDPNSYPAD 60

Query: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120
           KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA
Sbjct: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120

Query: 121 ALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ 179
           ALTVKAYGDLEKWVNIA+TDVRTCGTELGSERPPLLTERN+VFIKLCEIALTISKIAQ
Sbjct: 121 ALTVKAYGDLEKWVNIAITDVRTCGTELGSERPPLLTERNEVFIKLCEIALTISKIAQ 178

[Close]

BLAST of CmaCh14G009140 vs. ExPASy TrEMBL
Match: A0A0A0KY96 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001930 PE=4 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 1.2e-55
Identity = 116/180 (64.44%), Postives = 150/180 (83.33%), Query Frame = 0


Query: 1   MKFISAIVVILCLCMATKRHDATRPV-EEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPA 60
           MKF SAI+++LCLCMA +R DA     EEG+ +I++MC+QT +K+LC+ SLTSDPNS+PA
Sbjct: 1   MKFFSAILLVLCLCMAPQRLDAAGAAQEEGLGMIQKMCAQTNYKDLCITSLTSDPNSFPA 60

Query: 61  DKTGLALVALRFASSNASDISESIKVMFND-STLSKPMVQEALLDCLDEYLDASQQLDDS 120
           DK GLALVALR ASSNASDISESIKVM N+ S  ++P VQ+AL DCLDEYL+ASQQLDDS
Sbjct: 61  DKMGLALVALRLASSNASDISESIKVMLNETSQNNEPTVQQALFDCLDEYLEASQQLDDS 120

Query: 121 IAALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ 179
           IAA+  KAYGD+++WV +A+T+VRTC +   + +P +LT RN+ FIKLC+IAL+I+KIA+
Sbjct: 121 IAAIIAKAYGDVQEWVRVAVTNVRTCESSFPT-KPSVLTPRNEEFIKLCDIALSITKIAE 179

[Close]

BLAST of CmaCh14G009140 vs. ExPASy TrEMBL
Match: A0A5D3E196 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001210 PE=4 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 6.6e-51
Identity = 108/165 (65.45%), Postives = 136/165 (82.42%), Query Frame = 0


Query: 15  MATKRHD-ATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLALVALRFAS 74
           MA +R D A++  +EG+  IE+MC+QT +K+LCV SLTSDPNS+PADK GLALVALR AS
Sbjct: 1   MAAQRLDAASQANKEGIEKIEKMCAQTNYKDLCVTSLTSDPNSFPADKMGLALVALRLAS 60

Query: 75  SNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIAALTVKAYGDLEKW 134
           SNASDISESIKVM N+++ ++P VQ+ L DCLDEYLDASQQLDDSIAA+  KAYGD+EKW
Sbjct: 61  SNASDISESIKVMLNETSQNEPAVQQGLFDCLDEYLDASQQLDDSIAAIIAKAYGDVEKW 120

Query: 135 VNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ 179
           V+ A+ DVRTC     + +P +LT RN+ FIKLC+IAL+IS+IA+
Sbjct: 121 VHAAVADVRTCENSFPT-KPSVLTPRNEEFIKLCDIALSISRIAE 164

[Close]

BLAST of CmaCh14G009140 vs. ExPASy TrEMBL
Match: A0A6J1DPW6 (pectinesterase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111022446 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 2.4e-48
Identity = 111/174 (63.79%), Postives = 134/174 (77.01%), Query Frame = 0


Query: 6   AIVVILCLCMATKRHDATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLA 65
           A++V++ L +A    +A RP +  M LIE+ CSQT+H  LC+ SL SDPNS+PADKTGLA
Sbjct: 8   AMLVVVWLSVAAAA-EAIRPGKPDMGLIEQTCSQTQHPGLCLSSLKSDPNSFPADKTGLA 67

Query: 66  LVALRFASSNASDISESIKVMFNDSTLS-KPMVQEALLDCLDEYLDASQQLDDSIAALTV 125
           LVALR  SSNASDISE IKVM ND+ L+ +P VQ+ L DCLDEYLDASQQLDDSIAAL V
Sbjct: 68  LVALRLVSSNASDISEGIKVMLNDTRLADQPGVQQGLYDCLDEYLDASQQLDDSIAALLV 127

Query: 126 KAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ 179
           KAYGD++KWVN A+  VRTC     S +P +LT RND FIKLC+ ALTI+ IA+
Sbjct: 128 KAYGDVDKWVNAAVAAVRTCEASF-SVKPSVLTPRNDNFIKLCQNALTIAHIAE 179

[Close]

BLAST of CmaCh14G009140 vs. NCBI nr
Match: XP_022984074.1 (pectinesterase inhibitor-like [Cucurbita maxima])

HSP 1 Score: 348.6 bits (893), Expect = 4.2e-92
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 0


Query: 1   MKFISAIVVILCLCMATKRHDATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPAD 60
           MKFISAIVVILCLCMATKRHDATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPAD
Sbjct: 1   MKFISAIVVILCLCMATKRHDATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPAD 60

Query: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120
           KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA
Sbjct: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120

Query: 121 ALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ 179
           ALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ
Sbjct: 121 ALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ 178

[Close]

BLAST of CmaCh14G009140 vs. NCBI nr
Match: XP_022934353.1 (pectinesterase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 340.9 bits (873), Expect = 8.8e-90
Identity = 172/178 (96.63%), Postives = 176/178 (98.88%), Query Frame = 0


Query: 1   MKFISAIVVILCLCMATKRHDATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPAD 60
           MKF+SAIVV+LCLCMA KRHDATRPVEEGM LIEEMCSQTKHKELCVDSLTSDPNSYPAD
Sbjct: 1   MKFVSAIVVVLCLCMAAKRHDATRPVEEGMALIEEMCSQTKHKELCVDSLTSDPNSYPAD 60

Query: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120
           KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA
Sbjct: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120

Query: 121 ALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ 179
           ALTVKAYGDLEKWVNIA+TDVRTCGTELGSERPPLLTERN+VFIKLCEIALTISKIAQ
Sbjct: 121 ALTVKAYGDLEKWVNIAITDVRTCGTELGSERPPLLTERNEVFIKLCEIALTISKIAQ 178

[Close]

BLAST of CmaCh14G009140 vs. NCBI nr
Match: KAG6581280.1 (Pectinesterase inhibitor, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018002.1 Pectinesterase inhibitor, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 339.3 bits (869), Expect = 2.6e-89
Identity = 172/178 (96.63%), Postives = 175/178 (98.31%), Query Frame = 0


Query: 1   MKFISAIVVILCLCMATKRHDATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPAD 60
           MKFISAIVV+LCLCMA KRHDATRPVEEGM LIEEMCSQTKHKELCVDSLTSDPNSYPAD
Sbjct: 1   MKFISAIVVVLCLCMAAKRHDATRPVEEGMVLIEEMCSQTKHKELCVDSLTSDPNSYPAD 60

Query: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120
           KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA
Sbjct: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120

Query: 121 ALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ 179
           ALTVKAYGDLEKWVNIA+TDVRTCGTELG ERPPLLTERN+VFIKLCEIALTISKIAQ
Sbjct: 121 ALTVKAYGDLEKWVNIAITDVRTCGTELGRERPPLLTERNEVFIKLCEIALTISKIAQ 178

[Close]

BLAST of CmaCh14G009140 vs. NCBI nr
Match: XP_023528768.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 334.3 bits (856), Expect = 8.2e-88
Identity = 169/173 (97.69%), Postives = 171/173 (98.84%), Query Frame = 0


Query: 6   AIVVILCLCMATKRHDATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLA 65
           AIVV+LCLCMA KRHDATRPVEEGM LIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLA
Sbjct: 8   AIVVVLCLCMAAKRHDATRPVEEGMALIEEMCSQTKHKELCVDSLTSDPNSYPADKTGLA 67

Query: 66  LVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIAALTVK 125
           LVALRFASSNASDISESIKVMFNDSTL+KPMVQEALLDCLDEYLDASQQLDDSIAALTVK
Sbjct: 68  LVALRFASSNASDISESIKVMFNDSTLAKPMVQEALLDCLDEYLDASQQLDDSIAALTVK 127

Query: 126 AYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ 179
           AYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ
Sbjct: 128 AYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIAQ 180

[Close]

BLAST of CmaCh14G009140 vs. NCBI nr
Match: XP_038906317.1 (cell wall / vacuolar inhibitor of fructosidase 2-like [Benincasa hispida])

HSP 1 Score: 246.5 bits (628), Expect = 2.3e-61
Identity = 122/177 (68.93%), Postives = 149/177 (84.18%), Query Frame = 0


Query: 1   MKFISAIVVILCLCMATKRHDATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPAD 60
           MKF+SAI+ +LCL M  +R DA  P E+G+++I+ MC+QT +K+LCV SLTSDPNS+PAD
Sbjct: 1   MKFVSAILFVLCLSMVAQRLDAASPAEDGLSMIDHMCAQTNYKDLCVISLTSDPNSFPAD 60

Query: 61  KTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIA 120
           K GLALVALRFASSNASDISESIKVM ND++L+KP VQ+ L DCLDEYLDASQQLDD+IA
Sbjct: 61  KMGLALVALRFASSNASDISESIKVMLNDTSLNKPSVQQGLFDCLDEYLDASQQLDDAIA 120

Query: 121 ALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPLLTERNDVFIKLCEIALTISKIA 178
           A+ VKAYGD+EKWV+ A+  VRTC  E  + +P LL  +NDVFIKLCEIAL+ISK+A
Sbjct: 121 AILVKAYGDVEKWVDAAIASVRTCENEFAT-KPSLLAPKNDVFIKLCEIALSISKVA 176

[Close]

BLAST of CmaCh14G009140 vs. TAIR 10
Match: AT1G55770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 102.8 bits (255), Expect = 3.7e-22
Identity = 62/176 (35.23%), Postives = 104/176 (59.09%), Query Frame = 0


Query: 5   SAIVVILCLCMATKRHDATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNS--YPADKT 64
           SA V+ LC+ + +     T     GM  I ++C  +    LC+ S+TS P S  +     
Sbjct: 4   SAGVLFLCIVLIS---FVTGNANSGM--ISDLCKHSDDPNLCLSSITSRPESGEFAGTSN 63

Query: 65  GLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSIAAL 124
            + ++A+  AS+NAS  S  IK   ++  L +P +++ L DC  +Y DA +QLDDSI+A+
Sbjct: 64  QIEIIAISAASANASATSSYIKQKLSNEDL-EPAIEDTLEDCQKDYQDAVEQLDDSISAM 123

Query: 125 TVKAYGDLEKWVNIAMTDVRTCGTELGSE--RPPLLTERNDVFIKLCEIALTISKI 177
              A+ D++ W++ A++ + +CG+ LGS       L++RN+VF+KLC+ AL I+K+
Sbjct: 124 LADAHTDVDVWLSAAISAIESCGSALGSRAGNDAELSQRNEVFLKLCKNALMINKM 173

[Close]

BLAST of CmaCh14G009140 vs. TAIR 10
Match: AT4G02250.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 90.1 bits (222), Expect = 2.5e-18
Identity = 56/136 (41.18%), Postives = 74/136 (54.41%), Query Frame = 0


Query: 43  KELCVDSLTSDPNSYPADKTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALL 102
           K  CV SLTS P +  A    L ++AL  ASSNASD S  IK       L +P +++ L 
Sbjct: 23  KAFCVASLTSRPEAATATAPKLGVIALSIASSNASDTSFYIKAKLKQKNL-EPALEDTLD 82

Query: 103 DCLDEYLDASQQLDDSIAALTVKAYGDLEKWVNIAMTDVRTCGTELGSE--RPPLLTERN 162
           DC   YLDA  QLDDS+AAL   ++ D++ W+N A++D   C   L         L  RN
Sbjct: 83  DCSKNYLDAVAQLDDSLAALMQNSFIDVDIWLNTAISDGEACENALNDRAGNDAELARRN 142

Query: 163 DVFIKLCEIALTISKI 177
              +KLC+ AL I+ I
Sbjct: 143 TNLLKLCKDALLINTI 157

[Close]

BLAST of CmaCh14G009140 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 9.9e-07
Identity = 47/184 (25.54%), Postives = 83/184 (45.11%), Query Frame = 0


Query: 1   MKFISAIVVILCLCMATKRHDATRPVEEGMTLIEEMCSQT-----KHK-ELCVDSLTSDP 60
           MKF+  +V    L         +  +  G TLI   C +      K K  LCV SL ++P
Sbjct: 1   MKFLLYLVTFFVL---------SNGLANGQTLIRNSCKKATATSPKFKYNLCVTSLETNP 60

Query: 61  NSYPA-DKTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQ 120
            +  A D  GL + + + A + A+ +  ++  +     ++K M    L DCL  Y DA  
Sbjct: 61  QAKTAKDLAGLVMASTKNAVTKATTLKGTVDKIIKGKKVNK-MTAMPLRDCLQLYTDAIG 120

Query: 121 QLDDSIAALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPL-LTERNDVFIKLCEIALT 177
            L++++A +  + Y  ++  ++ AM    TC T     + P  +T+ ND   ++  I L 
Sbjct: 121 SLNEALAGVKSRNYPTVKTVLSAAMDTPSTCETGFKERKAPSPVTKENDNLYQMILIPLA 174

[Close]

BLAST of CmaCh14G009140 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 45.1 bits (105), Expect = 9.3e-05
Identity = 55/188 (29.26%), Postives = 82/188 (43.62%), Query Frame = 0


Query: 1   MKFISAIVVILCL--CMATKRHDATRPVEEGMTLIEEMCSQTKHK------ELCVDSLTS 60
           MKF   +V+   L  C AT             +LI + C +   K      + CV SL  
Sbjct: 1   MKFSLYLVMFFLLLNCFAT-----------AQSLIRDSCKKATTKDPKLKYDFCVKSLEE 60

Query: 61  DPNSYPADKTGLALVALRFASS-NASDISESIKVMFNDSTL--SKPMVQEALLDCLDEYL 120
           +P S    KT  +L  L F S+ NA   + S+K M  D  L   K  V+  LLDCL+ Y 
Sbjct: 61  NPQS----KTAKSLEGLVFVSTKNAVSKTTSLKGMV-DKILKEDKYEVERPLLDCLELYT 120

Query: 121 DASQQLDDSIAALTVKAYGDLEKWVNIAMTDVRTCGTELGSERPPL---LTERNDV--FI 173
           +A   L+ S+  +  + Y      ++ AM    +C T+    +  +    T+ NDV  ++
Sbjct: 121 EAIDSLNQSLDTVKSRDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYM 172

[Close]

BLAST of CmaCh14G009140 vs. TAIR 10
Match: AT1G53840.1 (pectin methylesterase 1 )

HSP 1 Score: 43.9 bits (102), Expect = 2.1e-04
Identity = 38/154 (24.68%), Postives = 69/154 (44.81%), Query Frame = 0


Query: 7   IVVILCLCMATKRH-------DATRPVEEGMTLIEEMCSQTKHKELCVDSLTSDPNSYPA 66
           I VI+   +AT  H        +  P     T ++ +CS T+  E C+ S++  P+S   
Sbjct: 40  IAVIIAAVVATVVHKNKNESTPSPPPELTPSTSLKAICSVTRFPESCISSISKLPSSNTT 99

Query: 67  DKTGLALVALRFASSNASDISESIKVMFNDSTLSKPMVQEALLDCLDEYLDASQQLDDSI 126
           D   L  ++L+        IS+  + +  ++      ++ AL  C D   DA  +L+D++
Sbjct: 100 DPETLFKLSLKVIIDELDSISDLPEKLSKET--EDERIKSALRVCGDLIEDALDRLNDTV 159

Query: 127 AA---------LTVKAYGDLEKWVNIAMTDVRTC 145
           +A         L+     DL+ W++  +TD  TC
Sbjct: 160 SAIDDEEKKKTLSSSKIEDLKTWLSATVTDHETC 191

The following BLAST results are available for this feature:

BLAST of CmaCh14G009140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0J8J8	1.9e-07	24.56	Pectinesterase inhibitor 28 OS=Oryza sativa subsp. japonica OX=39947 GN=PMEI28 P...	[more]

BLAST of CmaCh14G009140 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J9E7	2.0e-92	100.00	pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111482494 PE=4 S...	[more]
A0A6J1F7F2	4.3e-90	96.63	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111441544 PE=4...	[more]
A0A0A0KY96	1.2e-55	64.44	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G001930 PE=4 S...	[more]
A0A5D3E196	6.6e-51	65.45	Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A6J1DPW6	2.4e-48	63.79	pectinesterase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111022446 PE=...	[more]

BLAST of CmaCh14G009140 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022984074.1	4.2e-92	100.00	pectinesterase inhibitor-like [Cucurbita maxima]	[more]
XP_022934353.1	8.8e-90	96.63	pectinesterase inhibitor-like [Cucurbita moschata]	[more]
KAG6581280.1	2.6e-89	96.63	Pectinesterase inhibitor, partial [Cucurbita argyrosperma subsp. sororia] >KAG70...	[more]
XP_023528768.1	8.2e-88	97.69	pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]
XP_038906317.1	2.3e-61	68.93	cell wall / vacuolar inhibitor of fructosidase 2-like [Benincasa hispida]	[more]

BLAST of CmaCh14G009140 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G55770.1	3.7e-22	35.23	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G02250.1	2.5e-18	41.18	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46940.1	9.9e-07	25.54	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	9.3e-05	29.26	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G53840.1	2.1e-04	24.68	pectin methylesterase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 28..173 e-value: 1.2E-25 score: 101.2
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 32..173 e-value: 4.2E-28 score: 98.6
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 5..176 e-value: 1.5E-38 score: 129.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 30..176 e-value: 6.9E-36 score: 125.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 30..175
None	No IPR available	PANTHER	PTHR31080:SF1	OS05G0360900 PROTEIN	coord: 11..176
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 11..176
None	No IPR available	CDD	cd15801	PMEI-like_1	coord: 32..177 e-value: 1.95742E-46 score: 149.404

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh14G009140.1	CmaCh14G009140.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity