CmaCh14G006220 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh14G006220 vs. ExPASy Swiss-Prot
Match: Q9LFR0 (Alpha-mannosidase 2 OS=Arabidopsis thaliana OX=3702 GN=GMII PE=1 SV=1)

HSP 1 Score: 1697.2 bits (4394), Expect = 0.0e+00
Identity = 829/1176 (70.49%), Postives = 962/1176 (81.80%), Query Frame = 0

Query: 1    MAFSSFTG-------GGGSRRGGWASSILPFSSASPSAKPKHNRKYRRRLAIRDFIFANI 60
            M FSS+ G       GGG+  GGW  S+LP  +A   +K   NRK R+R  + +FIFAN 
Sbjct: 1    MPFSSYIGNSRRSSTGGGT--GGWGQSLLP--TALSKSKLAINRKPRKRTLVVNFIFANF 60

Query: 61   FTIGLLVSFIFFFIVLIRYGVPKPISSH-IKSHATRSHRQRKPIVSENWNSEVLSSNVDI 120
            F I L VS +FF + L  +GVP PISS  + S + R  + RK I     N     + VDI
Sbjct: 61   FVIALTVSLLFFLLTLFHFGVPGPISSRFLTSRSNRIVKPRKNINRRPLNDSNSGAVVDI 120

Query: 121  TTKKLYDKIEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYE 180
            TTK LYD+IEFLD DGGPWKQGWRVTYK DEW+ EKLKIFVVPHSHNDPGWKLTV+EYY+
Sbjct: 121  TTKDLYDRIEFLDTDGGPWKQGWRVTYKDDEWEKEKLKIFVVPHSHNDPGWKLTVEEYYQ 180

Query: 181  KQSRHILNTIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGG 240
            +QSRHIL+TIV+ LS+DSRRKFIWEEMSYLE+WWRDAS  K+E+   LVK+GQLEIVGGG
Sbjct: 181  RQSRHILDTIVETLSKDSRRKFIWEEMSYLERWWRDASPNKQEALTKLVKDGQLEIVGGG 240

Query: 241  WVMNDEANSHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENM 300
            WVMNDEANSHYFAIIEQ+AEGNMWL +T+G++PKNSWAIDPFGYS TMAYLLRRMGFENM
Sbjct: 241  WVMNDEANSHYFAIIEQIAEGNMWLNDTIGVIPKNSWAIDPFGYSSTMAYLLRRMGFENM 300

Query: 301  LIQRTHYELKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQ 360
            LIQRTHYELKK+LAQHKNLE+IWRQSWDA ETTDIFVHMMPFYSYDIPHTCGPEPAICCQ
Sbjct: 301  LIQRTHYELKKDLAQHKNLEYIWRQSWDAMETTDIFVHMMPFYSYDIPHTCGPEPAICCQ 360

Query: 361  FDFARSRGSSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFR 420
            FDFAR RG  YELCPW + PVE   ENVQERA+ LLDQYRKKS LYRTNTLLIPLGDDFR
Sbjct: 361  FDFARMRGFKYELCPWGKHPVETTLENVQERALKLLDQYRKKSTLYRTNTLLIPLGDDFR 420

Query: 421  YVSIDEAEAQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGS 480
            Y+SIDEAEAQF+NYQ+LFD+INSNPSLNAEAKFGTLEDYFRT+R+EA+++NYS PGEVGS
Sbjct: 421  YISIDEAEAQFRNYQMLFDHINSNPSLNAEAKFGTLEDYFRTVREEADRVNYSRPGEVGS 480

Query: 481  SQISGFPSLSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQ 540
             Q+ GFPSLSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLE TLR AE++++ LLG C 
Sbjct: 481  GQVVGFPSLSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEHTLRGAEIMMSFLLGYCH 540

Query: 541  RSQCEKLPVGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSK 600
            R QCEK P  F+YKLTAARRNLALFQHHDGVTGTAKD+VV+DYG RMHTSLQDL IFMSK
Sbjct: 541  RIQCEKFPTSFTYKLTAARRNLALFQHHDGVTGTAKDYVVQDYGTRMHTSLQDLQIFMSK 600

Query: 601  GIEVLLGIRH--EKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTR 660
             IEVLLGIRH  EKSDQ+PS FE EQ+RSKYDA+ VHK I  +EG   +V+ FNP  QTR
Sbjct: 601  AIEVLLGIRHEKEKSDQSPSFFEAEQMRSKYDARPVHKPIAAREGNSHTVILFNPSEQTR 660

Query: 661  EEVAMIIVNRTDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYY 720
            EEV  ++VNR +++VLDSNWTC+ SQISPE QHD   +FTGRHR++WK S+PALGL+TY+
Sbjct: 661  EEVVTVVVNRAEISVLDSNWTCVPSQISPEVQHDDTKLFTGRHRLYWKASIPALGLRTYF 720

Query: 721  IANGLFDCEKPKPAKLKFFSTSTSLPCATPYICSKADGDVAVIGNQHQSLVFHGKQGLLH 780
            IANG  +CEK  P+KLK+ S     PC  PY CSK D DV  I N+HQ+LVF  K G L 
Sbjct: 721  IANGNVECEKATPSKLKYASEFDPFPCPPPYSCSKLDNDVTEIRNEHQTLVFDVKNGSLR 780

Query: 781  KIINKNGPQNVVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYP 840
            KI+++NG + VV EEI +Y+S  SGAYLFKP+G+A+ I++  G  V +EG L+QEV+SYP
Sbjct: 781  KIVHRNGSETVVGEEIGMYSSPESGAYLFKPDGEAQPIVQPDGHVVTSEGLLVQEVFSYP 840

Query: 841  KTGWEKSPISHSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFY 900
            KT WEKSP+S  TRLY+GGNT+Q+ ++E+EYHVELLG +FDD+ELIVRYKTD+DNK++FY
Sbjct: 841  KTKWEKSPLSQKTRLYTGGNTLQDQVVEIEYHVELLGNDFDDRELIVRYKTDVDNKKVFY 900

Query: 901  SDLNGFQISRRESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEI 960
            SDLNGFQ+SRRE+YDKIPLQGNYYPMPSLAF++GSNGQRFSVHSRQSLGVASLK+GWLEI
Sbjct: 901  SDLNGFQMSRRETYDKIPLQGNYYPMPSLAFIQGSNGQRFSVHSRQSLGVASLKEGWLEI 960

Query: 961  MLDRRLSMDDGRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSLCIGA 1020
            MLDRRL  DDGRGLGQGV DNRAM V+FH+  ES++S + +P SN +P +PSLLS  IGA
Sbjct: 961  MLDRRLVRDDGRGLGQGVMDNRAMTVVFHLLAESNIS-QADPASNTNPRNPSLLSHLIGA 1020

Query: 1021 RLNYPLHTFIGKKPQASSVQ-PSPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPED-P 1080
             LNYP++TFI KKPQ  SV+ P   SF+PLA PLPCDLHIV+FKVPRP KYSQQ  ED P
Sbjct: 1021 HLNYPINTFIAKKPQDISVRVPQYGSFAPLAKPLPCDLHIVNFKVPRPSKYSQQLEEDKP 1080

Query: 1081 RFLLIFHRRHWDSSYCKTARS-NCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEM 1140
            RF LI +RR WDS+YC   R  NCT  ++EPVN  +MFK LA S  + TSLNLL ED E+
Sbjct: 1081 RFALILNRRAWDSAYCHKGRQVNCTSMANEPVNFSDMFKDLAASKVKPTSLNLLQEDMEI 1140

Query: 1141 LGYNEQ----SGDVAHEGQLHIPPMEIQAYKLELKP 1160
            LGY++Q          EG++ I PMEI+AYKLEL+P
Sbjct: 1141 LGYDDQELPRDSSQPREGRVSISPMEIRAYKLELRP 1171

BLAST of CmaCh14G006220 vs. ExPASy Swiss-Prot
Match: P49641 (Alpha-mannosidase 2x OS=Homo sapiens OX=9606 GN=MAN2A2 PE=2 SV=3)

HSP 1 Score: 773.5 bits (1996), Expect = 3.4e-222
Identity = 446/1062 (42.00%), Postives = 626/1062 (58.95%), Query Frame = 0

Query: 119  DKIEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHI 178
            +++ F +VDGG W+QG+ ++Y   +WD+E L++FVVPHSHNDPGW  T D+YY +Q++HI
Sbjct: 136  EELPFDNVDGGVWRQGFDISYDPHDWDAEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHI 195

Query: 179  LNTIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDE 238
            LN++V  L +D RR+F+W E+S+  KWW + + +K+ +   LV NGQLEI  GGWVM DE
Sbjct: 196  LNSMVSKLQEDPRRRFLWAEVSFFAKWWDNINVQKRAAVRRLVGNGQLEIATGGWVMPDE 255

Query: 239  ANSHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTH 298
            ANSHYFA+I+Q+ EG+ WL+  +G  P++ WA+DPFGYS TM YLLRR    +MLIQR H
Sbjct: 256  ANSHYFALIDQLIEGHQWLERNLGATPRSGWAVDPFGYSSTMPYLLRRANLTSMLIQRVH 315

Query: 299  YELKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARS 358
            Y +KK  A   +LEF+WRQ+WD++ +TDIF HMMPFYSYD+PHTCGP+P ICCQFDF R 
Sbjct: 316  YAIKKHFAATHSLEFMWRQTWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRL 375

Query: 359  RGSSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDE 418
             G     CPW+  P  I + NV ERA +LLDQYRKKS L+R+N LL+PLGDDFRY    E
Sbjct: 376  PGGRIN-CPWKVPPRAITEANVAERAALLLDQYRKKSQLFRSNVLLVPLGDDFRYDKPQE 435

Query: 419  AEAQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGF 478
             +AQF NYQ LFD+ NS P+L+ +A+FGTL DYF  L        Y   G    ++  GF
Sbjct: 436  WDAQFFNYQRLFDFFNSRPNLHVQAQFGTLSDYFDAL--------YKRTGVEPGARPPGF 495

Query: 479  PSLSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRS-QCE 538
            P LSGDFF+YADR+  YW+GYY SRPF+K++DRVLE  LR AE++ +L     +RS    
Sbjct: 496  PVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEVLYSLAAAHARRSGLAG 555

Query: 539  KLPVGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDL-HIFMSKGIEV 598
            + P+     LT ARR L LFQHHD +TGTAK+ VV DYGVR+  SL +L  + +     +
Sbjct: 556  RYPLSDFTLLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVNLKQVIIHAAHYL 615

Query: 599  LLGIRHEKSDQNPSQFEPE-------QVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQT 658
            +LG      D+    F+PE         R  +DA      I L +   + VV FNPL Q 
Sbjct: 616  VLG------DKETYHFDPEAPFLQVDDTRLSHDALPERTVIQL-DSSPRFVVLFNPLEQE 675

Query: 659  REEVAMIIVNRTDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTY 718
            R  +  ++VN   V VL      L  QIS  +           +++   + +PALGL   
Sbjct: 676  RFSMVSLLVNSPRVRVLSEEGQPLAVQISAHWS-SATEAVPDVYQVSVPVRLPALGLGVL 735

Query: 719  YIANGLFDCEKPKPAKLKFF----STSTSLPCATPYICSKADGDVAVIGNQHQSLVFHGK 778
             +  GL D  +  P+ ++ +      S S   A P     +      + N++  + F G 
Sbjct: 736  QLQLGL-DGHRTLPSSVRIYLHGRQLSVSRHEAFPLRVIDSGTSDFALSNRYMQVWFSGL 795

Query: 779  QGLLHKI--INKNGPQNVVNEEIALYTSWG---SGAYLFKPNGDAESII-KAGGLTVITE 838
             GLL  I  +++   Q V  + +   T      SGAYLF P+G+A+  + K   +  +TE
Sbjct: 796  TGLLKSIRRVDEEHEQQVDMQVLVYGTRTSKDKSGAYLFLPDGEAKPYVPKEPPVLRVTE 855

Query: 839  GPLMQEVYSYPKTGWEKSPISHSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRY 898
            GP   EV +Y         I  + RLY+    ++   +++   V++  +++ +KEL +  
Sbjct: 856  GPFFSEVVAY------YEHIHQAVRLYNLPG-VEGLSLDISSLVDI--RDYVNKELALHI 915

Query: 899  KTDIDNKRIFYSDLNGFQISRRESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLG 958
             TDID++ IF++DLNGFQ+  R    K+PLQ N+YPMP +A+++ +  +R ++H+ Q+LG
Sbjct: 916  HTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYIQDAQ-KRLTLHTAQALG 975

Query: 959  VASLKDGWLEIMLDRRLSMDDGRGLGQGVRDNRAMNVIFHISLE-SSVSTKLNPVSNYSP 1018
            V+SLKDG LE++LDRRL  DD RGLGQG++DN+     F + LE  +V +++    ++S 
Sbjct: 976  VSSLKDGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNRFRLLLERRTVGSEVQ--DSHST 1035

Query: 1019 LSPSLLSLCIGARLNYPLHTFIGKKPQASSVQPSP--RSFSPLAAPLPCDLHIVSFKVPR 1078
              PSLLS      LN P        P A    P P  RSF PLA+ LPCD H+++    R
Sbjct: 1036 SYPSLLSHLTSMYLNAPALAL----PVARMQLPGPGLRSFHPLASSLPCDFHLLNL---R 1095

Query: 1079 PLKYSQQSPEDPRFLLIFHRRHWDSSY-CKTARSNCTRFSDEPVNIFNMFKGLAVSNARA 1138
             L+  + +       LI HR+ +D     K    NCT  S   V + ++F GL V   + 
Sbjct: 1096 TLQAEEDTLPSAETALILHRKGFDCGLEAKNLGFNCTT-SQGKVALGSLFHGLDVVFLQP 1149

Query: 1139 TSLNLLHEDTEMLGYNEQSGDVAHEGQLHIPPMEIQAYKLEL 1158
            TSL LL+     L     S DV      ++ PMEI  ++L L
Sbjct: 1156 TSLTLLYP----LASPSNSTDV------YLEPMEIATFRLRL 1149

BLAST of CmaCh14G006220 vs. ExPASy Swiss-Prot
Match: Q8BRK9 (Alpha-mannosidase 2x OS=Mus musculus OX=10090 GN=Man2a2 PE=1 SV=2)

HSP 1 Score: 763.8 bits (1971), Expect = 2.7e-219
Identity = 438/1060 (41.32%), Postives = 625/1060 (58.96%), Query Frame = 0

Query: 119  DKIEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHI 178
            + + F +V+GG W+QG+ ++Y  ++WD+E L++FVVPHSHNDPGW  T D+YY +Q++HI
Sbjct: 136  EDLPFDNVEGGVWRQGFDISYSPNDWDTEDLQVFVVPHSHNDPGWIKTFDKYYTEQTQHI 195

Query: 179  LNTIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDE 238
            LN++V  L +D RR+F+W E+S+  KWW + S +K+ +   LV NGQLEI  GGWVM DE
Sbjct: 196  LNSMVSKLQEDPRRRFLWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDE 255

Query: 239  ANSHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTH 298
            ANSHYFA+++Q+ EG+ WL+  +G  P++ WA+DPFG+S TM YLLRR    +MLIQR H
Sbjct: 256  ANSHYFALVDQLIEGHQWLERNLGATPRSGWAVDPFGHSSTMPYLLRRANLTSMLIQRVH 315

Query: 299  YELKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARS 358
            Y +KK  A   +LEF+WRQ WD++ +TDIF HMMPFYSYD+PHTCGP+P ICCQFDF R 
Sbjct: 316  YAIKKHFAATHSLEFMWRQMWDSDSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRL 375

Query: 359  RGSSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDE 418
             G     CPW+  P  I + NV +RA +LLDQYRKKS L+R+N LL+PLGDDFRY    E
Sbjct: 376  PGGRIN-CPWKVPPRAITEANVADRAALLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQE 435

Query: 419  AEAQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGF 478
             +AQF NYQ LFD++NS P  + +A+FGTL +YF  L        Y   G    ++  GF
Sbjct: 436  WDAQFFNYQRLFDFLNSKPEFHVQAQFGTLSEYFDAL--------YKRTGVEPGARPPGF 495

Query: 479  PSLSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRS-QCE 538
            P LSGDFF+YADR+  YW+GYY SRPF+K++DRVLE  LR AE++ +L L   +RS    
Sbjct: 496  PVLSGDFFSYADREDHYWTGYYTSRPFYKSLDRVLEAHLRGAEILYSLALAHARRSGLAG 555

Query: 539  KLPVGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDL-HIFMSKGIEV 598
            + P+     LT ARR L LFQHHD +TGTAK+ VV DYGVR+  SL  L  + ++    +
Sbjct: 556  QYPLSDFALLTEARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIINAAHYL 615

Query: 599  LLGIRHEKSDQNPSQFEP-------EQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQT 658
            +LG      DQ    F+P       +  R  +DA      I L +   + VV FNPL Q 
Sbjct: 616  VLG------DQETYSFDPGTPFLQMDDSRVSHDALPERTVIRL-DSSPRFVVVFNPLEQE 675

Query: 659  REEVAMIIVNRTDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTY 718
            R  V  ++VN   V VL      L  QIS  +     ++    +++   + +P LGL   
Sbjct: 676  RLSVVSLLVNSPRVRVLSEEGQPLSVQISVHWS-SATDMVPDVYQVSVPVRLPGLGLGVL 735

Query: 719  YI---ANGLFDCEKPKPAKLKFFSTSTSLPCATPYICSKADGDVAVIGNQHQSLVFHGKQ 778
             +    +G +  +      L     S S   A P     +      I N++  + F G  
Sbjct: 736  QLQPDLDGPYTLQSSVRVYLNGVKLSVSRQSAFPVRVVDSGASDFAISNRYMQVWFSGLT 795

Query: 779  GLLHKI--INKNGPQNVVNEEIALYTSWG---SGAYLFKPNGDAESII-KAGGLTVITEG 838
            GLL  I  +++   Q +  E +   T      SGAYLF P+ +A+  + K   +  +TEG
Sbjct: 796  GLLKSIRRVDEEQEQQMELEFLVYGTRTSKDKSGAYLFLPDSEAKPYVPKKPPVLRVTEG 855

Query: 839  PLMQEVYSYPKTGWEKSPISHSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYK 898
            P   EV  Y              RLY+    ++   ++M + V++  +++ +KEL +R  
Sbjct: 856  PFFSEVAVY------YEHFHQVIRLYNLPG-VEGLSLDMSFQVDI--RDYVNKELALRIH 915

Query: 899  TDIDNKRIFYSDLNGFQISRRESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGV 958
            TDID++  F++DLNGFQI  R+   K+PLQ N+YPMP +A+++ S  +R ++H+ Q+LGV
Sbjct: 916  TDIDSQGTFFTDLNGFQIQPRQYLKKLPLQANFYPMPVMAYIQDSQ-RRLTLHTAQALGV 975

Query: 959  ASLKDGWLEIMLDRRLSMDDGRGLGQGVRDNRAMNVIFHISLE--SSVSTKLNPVSNYSP 1018
            +SL +G LE++LDRRL  DD RGLGQG++DN+     F + LE  +++S +++     S 
Sbjct: 976  SSLGNGQLEVILDRRLMQDDNRGLGQGLKDNKITCNRFRLLLERRTTMSPEVHQEQERST 1035

Query: 1019 LSPSLLSLCIGARLNYPLHTFIGKKPQASSVQPSPRSFSPLAAPLPCDLHIVSFKVPRPL 1078
              PSLLS      L+ P       K Q +S  P+ RSF PLA+PLPCD H+++ ++  P 
Sbjct: 1036 SYPSLLSHLTSMYLSTPPLVLPVAKRQGTS--PALRSFHPLASPLPCDFHLLNLRM-LPA 1095

Query: 1079 KYSQQSPEDPRFLLIFHRRHWDSSY-CKTARSNCTRFSDEPVNIFNMFKGLAVSNARATS 1138
            + +  + +     LI HR+ +D     K    NCT  S   + + ++F GL V+  + TS
Sbjct: 1096 EDTLPATDS---ALILHRKGFDCGLEAKNLGFNCTT-SQGKLALGSLFHGLDVTFLQPTS 1151

Query: 1139 LNLLHEDTEMLGYNEQSGDVAHEGQLHIPPMEIQAYKLEL 1158
            L LL+     L     S D++ E      PMEI  ++L L
Sbjct: 1156 LTLLYP----LASPSNSTDISLE------PMEISTFRLRL 1151

BLAST of CmaCh14G006220 vs. ExPASy Swiss-Prot
Match: P27046 (Alpha-mannosidase 2 OS=Mus musculus OX=10090 GN=Man2a1 PE=1 SV=2)

HSP 1 Score: 738.8 bits (1906), Expect = 9.4e-212
Identity = 422/1064 (39.66%), Postives = 601/1064 (56.48%), Query Frame = 0

Query: 111  DITTKKLYDKIEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEY 170
            D+    +YD I F + DGG WKQG+ + Y+ DEWD E L++FVVPHSHNDPGW  T ++Y
Sbjct: 127  DVQMLDVYDLIPFDNPDGGVWKQGFDIKYEADEWDHEPLQVFVVPHSHNDPGWLKTFNDY 186

Query: 171  YEKQSRHILNTIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVG 230
            +  ++++I N +V  L +DS RKF+W E+SYL KWW      KKE+  +L++NGQLEIV 
Sbjct: 187  FRDKTQYIFNNMVLKLKEDSSRKFMWSEISYLAKWWDIIDIPKKEAVKSLLQNGQLEIVT 246

Query: 231  GGWVMNDEANSHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFE 290
            GGWVM DEA  HYFA+I+Q+ EG+ WL++ +G+ P++ WAIDPFG+SPTMAYLL+R GF 
Sbjct: 247  GGWVMPDEATPHYFALIDQLIEGHQWLEKNLGVKPRSGWAIDPFGHSPTMAYLLKRAGFS 306

Query: 291  NMLIQRTHYELKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAIC 350
            +MLIQR HY +KK  + HK LEF WRQ+WD    TDI  HMMPFYSYDIPHTCGP+P IC
Sbjct: 307  HMLIQRVHYAIKKHFSLHKTLEFFWRQNWDLGSATDILCHMMPFYSYDIPHTCGPDPKIC 366

Query: 351  CQFDFARSRGSSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDD 410
            CQFDF R  G  Y  CPW   P  I+  NVQ RA MLLDQYRKKS L+RT  LL PLGDD
Sbjct: 367  CQFDFKRLPGGRYG-CPWGVPPEAISPGNVQSRAQMLLDQYRKKSKLFRTKVLLAPLGDD 426

Query: 411  FRYVSIDEAEAQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEV 470
            FR+    E + Q +NY+ LF Y+NS P L  + +FGTL DYF    D  EK   ++  E 
Sbjct: 427  FRFSEYTEWDLQCRNYEQLFSYMNSQPHLKVKIQFGTLSDYF----DALEK---AVAAEK 486

Query: 471  GSSQISGFPSLSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGS 530
             SSQ S FP+LSGDFFTYADR   YWSGY+ SRPF+K +DR++E  +RAAE++  L L  
Sbjct: 487  KSSQ-SVFPALSGDFFTYADRDDHYWSGYFTSRPFYKRMDRIMESRIRAAEILYQLALKQ 546

Query: 531  CQRSQCEKLPVGFSY-KLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLH-- 590
             Q+ +  K      Y  LT ARRNL LFQHHD +TGTAKD VV DYG R+  SL  L   
Sbjct: 547  AQKYKINKFLSSPHYTTLTEARRNLGLFQHHDAITGTAKDWVVVDYGTRLFQSLNSLEKI 606

Query: 591  IFMSKGIEVLLGIRHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLA 650
            I  S  + +L   +  +SD + +  E +  +S  D+      I L     + +V +NP  
Sbjct: 607  IGDSAFLLILKDKKLYQSDPSKAFLEMDTKQSSQDSLPQKIIIQLSAQEPRYLVVYNPFE 666

Query: 651  QTREEVAMIIVNRTDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQ 710
            Q R  V  I VN   V VL  +   ++ Q+S  + +D   +    + + +   +P LGL+
Sbjct: 667  QERHSVVSIRVNSATVKVLSDSGKPVEVQVSAVW-NDMRTISQAAYEVSFLAHIPPLGLK 726

Query: 711  TYYIANGLFDCEKPKPAKLKFFSTSTSLPCATPYICSKAD-GDVAVIGNQHQSLVFHGKQ 770
             + I            A    ++          ++ +  D GD   I N   ++ F  + 
Sbjct: 727  VFKILES--QSSSSHLADYVLYNNDGLAENGIFHVKNMVDAGDAITIENPFLAIWF-DRS 786

Query: 771  GLLHKIINK-NGPQNVVNEEIALYTSWG----SGAYLFKPNGDAESIIKAGGLTV-ITEG 830
            GL+ K+  K +  Q+ +  +   Y +      SGAYLF P+G  +  +      V +T G
Sbjct: 787  GLMEKVRRKEDSRQHELKVQFLWYGTTNKRDKSGAYLFLPDGQGQPYVSLRPPFVRVTRG 846

Query: 831  PLMQEVYSYPKTGWEKSPISHSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYK 890
             +  +V  +         ++H  RLY+    I+   +E+   V +  +   ++E+++R  
Sbjct: 847  RIYSDVTCF------LEHVTHKVRLYN-IQGIEGQSMEVSNIVNI--RNVHNREIVMRIS 906

Query: 891  TDIDNKRIFYSDLNGFQISRRESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGV 950
            + I+N+  +Y+DLNG+QI  R +  K+PLQ N YPM ++A+++ +   R ++ S QSLG 
Sbjct: 907  SKINNQNRYYTDLNGYQIQPRRTMSKLPLQANVYPMCTMAYIQDAE-HRLTLLSAQSLGA 966

Query: 951  ASLKDGWLEIMLDRRLSMDDGRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLS 1010
            +S+  G +E+ +DRRL  DD RGLGQGV DN+    +F I LE   +  +      SP+S
Sbjct: 967  SSMASGQIEVFMDRRLMQDDNRGLGQGVHDNKITANLFRILLEKRSAVNMEE-EKKSPVS 1026

Query: 1011 -PSLLSLCIGARLNYPLHTFIGKKPQASSVQPSP-----RSFSPLAAPLPCDLHIVSFKV 1070
             PSLLS    + LN+P    +      S   PSP       F  L + LPCD+H+V+   
Sbjct: 1027 YPSLLSHMTSSFLNHPFLPMV-----LSGQLPSPAFELLSEFPLLQSSLPCDIHLVNL-- 1086

Query: 1071 PRPLKYSQQSPEDPRFLLIFHRRHWDSSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNAR 1130
             R ++            LI HR+ +D  +           +   +++  +F   AV +  
Sbjct: 1087 -RTIQSKMGKGYSDEAALILHRKGFDCQFSSRGIGLPCSTTQGKMSVLKLFNKFAVESLV 1146

Query: 1131 ATSLNLLHEDTEMLGYNEQSGDVAHEGQLHIPPMEIQAYKLELK 1159
             +SL+L+H             D  +  ++ + PMEI  +++ L+
Sbjct: 1147 PSSLSLMHSPP----------DAQNMSEVSLSPMEISTFRIRLR 1148

BLAST of CmaCh14G006220 vs. ExPASy Swiss-Prot
Match: P28494 (Alpha-mannosidase 2 OS=Rattus norvegicus OX=10116 GN=Man2a1 PE=1 SV=2)

HSP 1 Score: 736.9 bits (1901), Expect = 3.6e-211
Identity = 426/1067 (39.93%), Postives = 597/1067 (55.95%), Query Frame = 0

Query: 111  DITTKKLYDKIEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEY 170
            D+    +YD I F + DGG WKQG+ + Y+ DEWD E L++FVVPHSHNDPGW  T ++Y
Sbjct: 127  DVQMLDVYDLIPFDNPDGGVWKQGFDIKYEADEWDREPLQVFVVPHSHNDPGWLKTFNDY 186

Query: 171  YEKQSRHILNTIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVG 230
            +  ++++I N +V  L +DS RKFIW E+SYL KWW    + KKE+  +L++NGQLEIV 
Sbjct: 187  FRDKTQYIFNNMVLKLKEDSSRKFIWSEISYLAKWWDIIDNPKKEAVKSLLQNGQLEIVT 246

Query: 231  GGWVMNDEANSHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFE 290
            GGWVM DEA +HYFA+I+Q+ EG+ WL++ +G+ P++ WAIDPFG+SPTM YLL+R GF 
Sbjct: 247  GGWVMADEATTHYFALIDQLIEGHQWLEKNLGVKPRSGWAIDPFGHSPTMTYLLKRAGFS 306

Query: 291  NMLIQRTHYELKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAIC 350
            +MLIQR HY +KK  +  K LEF WRQ+WD   TTDI  HMMPFYSYDIPHTCGP+P IC
Sbjct: 307  HMLIQRVHYSVKKHFSLQKTLEFFWRQNWDLGSTTDILCHMMPFYSYDIPHTCGPDPKIC 366

Query: 351  CQFDFARSRGSSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDD 410
            CQFDF R  G  Y  CPW   P  I+  NVQ RA MLLDQYRKKS L+RT  LL PLGDD
Sbjct: 367  CQFDFKRLPGGRYG-CPWGVPPEAISPGNVQSRAQMLLDQYRKKSKLFRTKVLLAPLGDD 426

Query: 411  FRYVSIDEAEAQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEV 470
            FR+    E + Q++NY+ LF Y+NS P L  + +FGTL DYF    D  EK   S+  E 
Sbjct: 427  FRFSEYTEWDLQYRNYEQLFSYMNSQPHLKVKIQFGTLSDYF----DALEK---SVAAEK 486

Query: 471  GSSQISGFPSLSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGS 530
               Q S FP+LSGDFFTYADR   YWSGY+ SRPF+K +DR++E  LR AE++  L L  
Sbjct: 487  KGGQ-SVFPALSGDFFTYADRDDHYWSGYFTSRPFYKRMDRIMESRLRTAEILYHLALKQ 546

Query: 531  CQRSQCEKLPVGFSY-KLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLH-- 590
             Q+ +  K      Y  LT ARRNL LFQHHD +TGTAKD VV DYG R+  SL  L   
Sbjct: 547  AQKYKINKFLSSPHYTTLTEARRNLGLFQHHDAITGTAKDWVVVDYGTRLFQSLNSLEKI 606

Query: 591  IFMSKGIEVLLGIRHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLA 650
            I  S  + +L   +  +SD + +  E +  +S  D+      I L     + +V +NP  
Sbjct: 607  IGDSAFLLILKDKKLYQSDPSKAFLEMDTKQSSQDSLPKKNIIQLSAQEPRYLVVYNPFE 666

Query: 651  QTREEVAMIIVNRTDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQ 710
            Q R  V  + VN   V VL      ++ Q+S  ++ D        + + +   +P LGL+
Sbjct: 667  QERHSVVSVRVNSATVKVLSDLGKAVEVQVSAVWK-DMRTTSQAAYEVAFLAHLPPLGLK 726

Query: 711  TYYIANGLFDCEKPKPAKLKFFSTSTSLPCATPYICSKAD-GDVAVIGNQHQSLVFHGKQ 770
             Y I            A    ++          ++ +  D GD   I N   +L F  + 
Sbjct: 727  VYKILES--QSSSSHLADYFLYNNDGQAESGIFHMKNMVDSGDAITIENSFLTLGF-DRS 786

Query: 771  GLLHKIINKNGPQNVVNEEIALYTSW--------GSGAYLFKPNGDAESIIKAGGLTV-I 830
            GL+ K+  K   ++   +E+ +   W         SGAYLF P+G  +  +      V +
Sbjct: 787  GLMEKVRRK---EDNKQQELKVQFLWYGTTNKRDKSGAYLFLPDGQGQPYVSLRTPFVRV 846

Query: 831  TEGPLMQEVYSYPKTGWEKSPISHSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIV 890
            T G +  +V  +         ++H  RLY     I+   +E+   V++  +   ++E+++
Sbjct: 847  TRGRIYSDVTCF------LEHVTHKVRLYH-IQGIEGQSMEVSNIVDI--RSVHNREIVM 906

Query: 891  RYKTDIDNKRIFYSDLNGFQISRRESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQS 950
            R  + I+N+  +Y+DLNG+QI  R +  K+PLQ N YPM ++A+++ +   R ++ S QS
Sbjct: 907  RISSKINNQNRYYTDLNGYQIQPRRTMAKLPLQANVYPMSTMAYIQDA-AHRLTLLSAQS 966

Query: 951  LGVASLKDGWLEIMLDRRLSMDDGRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYS 1010
            LG +S+  G +E+ +DRRL  DD RGLGQGV DN+    +F I LE      +      S
Sbjct: 967  LGASSMASGQIEVFMDRRLMQDDNRGLGQGVHDNKITANLFRILLEKRNGMNMEE-DKKS 1026

Query: 1011 PLS-PSLLSLCIGARLNYPLHTFIGKKPQASSVQPSP-----RSFSPLAAPLPCDLHIVS 1070
            P+S PSLLS    A LN+P    +      S   PSP       F  L + LPCD+H+V+
Sbjct: 1027 PVSYPSLLSHMTSAFLNHPFLPMV-----LSGQLPSPAIELLSEFRLLQSSLPCDIHLVN 1086

Query: 1071 FKVPRPLKYSQQSPEDPRFLLIFHRRHWDSSYCKTARSNCTRFSDEPVNIFNMFKGLAVS 1130
             +  +       S E     LI HR+ +D      A       +   ++I  +F   AV 
Sbjct: 1087 LRTIQSKVGKGYSDE---AALILHRKVFDCQLSSRAMGLPCSTTQGKMSIPKLFNNFAVE 1146

Query: 1131 NARATSLNLLHEDTEMLGYNEQSGDVAHEGQLHIPPMEIQAYKLELK 1159
            +   +SL+L+H             D  +  ++ + PMEI   ++ L+
Sbjct: 1147 SFIPSSLSLMHSPP----------DAQNTSEVSLSPMEISTSRIRLR 1148

BLAST of CmaCh14G006220 vs. ExPASy TrEMBL
Match: A0A6J1J326 (Alpha-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111482230 PE=3 SV=1)

HSP 1 Score: 2378.2 bits (6162), Expect = 0.0e+00
Identity = 1160/1160 (100.00%), Postives = 1160/1160 (100.00%), Query Frame = 0

Query: 1    MAFSSFTGGGGSRRGGWASSILPFSSASPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60
            MAFSSFTGGGGSRRGGWASSILPFSSASPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV
Sbjct: 1    MAFSSFTGGGGSRRGGWASSILPFSSASPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60

Query: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRQRKPIVSENWNSEVLSSNVDITTKKLYDK 120
            SFIFFFIVLIRYGVPKPISSHIKSHATRSHRQRKPIVSENWNSEVLSSNVDITTKKLYDK
Sbjct: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRQRKPIVSENWNSEVLSSNVDITTKKLYDK 120

Query: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180
            IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN
Sbjct: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180

Query: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240
            TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN
Sbjct: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240

Query: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
            SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420
            SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE
Sbjct: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420

Query: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS
Sbjct: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480

Query: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540
            LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP
Sbjct: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540

Query: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600
            VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI
Sbjct: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600

Query: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTREEVAMIIVNR 660
            RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTREEVAMIIVNR
Sbjct: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTREEVAMIIVNR 660

Query: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720
            TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK
Sbjct: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720

Query: 721  PKPAKLKFFSTSTSLPCATPYICSKADGDVAVIGNQHQSLVFHGKQGLLHKIINKNGPQN 780
            PKPAKLKFFSTSTSLPCATPYICSKADGDVAVIGNQHQSLVFHGKQGLLHKIINKNGPQN
Sbjct: 721  PKPAKLKFFSTSTSLPCATPYICSKADGDVAVIGNQHQSLVFHGKQGLLHKIINKNGPQN 780

Query: 781  VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840
            VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS
Sbjct: 781  VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840

Query: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900
            HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR
Sbjct: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960
            RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960

Query: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSLCIGARLNYPLHTFI 1020
            GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSLCIGARLNYPLHTFI
Sbjct: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSLCIGARLNYPLHTFI 1020

Query: 1021 GKKPQASSVQPSPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD 1080
            GKKPQASSVQPSPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD
Sbjct: 1021 GKKPQASSVQPSPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE
Sbjct: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140

Query: 1141 GQLHIPPMEIQAYKLELKPR 1161
            GQLHIPPMEIQAYKLELKPR
Sbjct: 1141 GQLHIPPMEIQAYKLELKPR 1160

BLAST of CmaCh14G006220 vs. ExPASy TrEMBL
Match: A0A6J1F199 (Alpha-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111441463 PE=3 SV=1)

HSP 1 Score: 2334.7 bits (6049), Expect = 0.0e+00
Identity = 1140/1160 (98.28%), Postives = 1144/1160 (98.62%), Query Frame = 0

Query: 1    MAFSSFTGGGGSRRGGWASSILPFSSASPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60
            MAFSSFTGGGGSRRGGWASSILPFSS SPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV
Sbjct: 1    MAFSSFTGGGGSRRGGWASSILPFSSVSPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60

Query: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRQRKPIVSENWNSEVLSSNVDITTKKLYDK 120
            SFIFFFIVLIRYGVPKPISSHIKSHATRSHR RKPIVS+NWNSEVLSS VDITTKKLYDK
Sbjct: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRPRKPIVSKNWNSEVLSSTVDITTKKLYDK 120

Query: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180
            IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN
Sbjct: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180

Query: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240
            TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN
Sbjct: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240

Query: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
            SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420
            SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE
Sbjct: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420

Query: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQI GFPS
Sbjct: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQIGGFPS 480

Query: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540
            LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP
Sbjct: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540

Query: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600
            VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI
Sbjct: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600

Query: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTREEVAMIIVNR 660
            RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEG YQSVVFFNPL QTREEVAMIIVNR
Sbjct: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGTYQSVVFFNPLEQTREEVAMIIVNR 660

Query: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720
            TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHR+HWKISVPALGLQTYYIANGLFDCEK
Sbjct: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRLHWKISVPALGLQTYYIANGLFDCEK 720

Query: 721  PKPAKLKFFSTSTSLPCATPYICSKADGDVAVIGNQHQSLVFHGKQGLLHKIINKNGPQN 780
            PKPAKLKFFSTSTSLPCATPYICSKAD DVAVI NQHQSLVF GK GLLHKIINKNG QN
Sbjct: 721  PKPAKLKFFSTSTSLPCATPYICSKADDDVAVIENQHQSLVFDGKHGLLHKIINKNGSQN 780

Query: 781  VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840
            VV+EEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS
Sbjct: 781  VVSEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840

Query: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900
            HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR
Sbjct: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960
            RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960

Query: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSLCIGARLNYPLHTFI 1020
            GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLS  IGARLNYPLHTFI
Sbjct: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSHGIGARLNYPLHTFI 1020

Query: 1021 GKKPQASSVQPSPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD 1080
             KKPQASSVQP PRSFSPLA PLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD
Sbjct: 1021 AKKPQASSVQPPPRSFSPLATPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE
Sbjct: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140

Query: 1141 GQLHIPPMEIQAYKLELKPR 1161
            GQLHIPPME+QAYKLELKPR
Sbjct: 1141 GQLHIPPMEVQAYKLELKPR 1160

BLAST of CmaCh14G006220 vs. ExPASy TrEMBL
Match: A0A1C9J7V4 (Alpha-mannosidase OS=Cucumis melo OX=3656 PE=2 SV=1)

HSP 1 Score: 2188.3 bits (5669), Expect = 0.0e+00
Identity = 1048/1160 (90.34%), Postives = 1101/1160 (94.91%), Query Frame = 0

Query: 1    MAFSSFTGGGGSRRGGWASSILPFSSASPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60
            MAFSSFTGG G+RRGGWA SILPFSS SPSAKPKHNRKYRRRLAIRDFIF+N FTIGLL+
Sbjct: 1    MAFSSFTGGSGARRGGWAGSILPFSSVSPSAKPKHNRKYRRRLAIRDFIFSNFFTIGLLI 60

Query: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRQRKPIVSENWNSEVLSSNVDITTKKLYDK 120
            SF FFFIVL+RYGVPKPISS  KSHA RSHR RKPIVSENWNSEVLSSNVDITTK+LYDK
Sbjct: 61   SFFFFFIVLLRYGVPKPISSPFKSHAVRSHRPRKPIVSENWNSEVLSSNVDITTKELYDK 120

Query: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180
            IEFLD+DGGPWKQGWRVTYKGDEWDSEKLK+FVVPHSHNDPGWKLTVDEYYE+QSRHIL+
Sbjct: 121  IEFLDIDGGPWKQGWRVTYKGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRHILD 180

Query: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240
            TIV+ALS+DSRRKFIWEEMSYLEKWWRDASDEKKESF  LVKNGQLEIVGGGWVMNDEAN
Sbjct: 181  TIVEALSRDSRRKFIWEEMSYLEKWWRDASDEKKESFAALVKNGQLEIVGGGWVMNDEAN 240

Query: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
            SHYFAIIEQMAEGN+WL ET+G+VPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNIWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELA HKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELALHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420
            S YELCPWRQDPVEINKENVQERA  LLDQYRKKSVLYRTNTLLIPLGDDFRY++IDEAE
Sbjct: 361  SLYELCPWRQDPVEINKENVQERATTLLDQYRKKSVLYRTNTLLIPLGDDFRYINIDEAE 420

Query: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFDYINS PSLNAEA FGTLEDYFRTLRDEAEKINYSLPGEVGSS + GFPS
Sbjct: 421  AQFKNYQLLFDYINSTPSLNAEANFGTLEDYFRTLRDEAEKINYSLPGEVGSSLVGGFPS 480

Query: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540
            LSGDFFTYADRQ+DYWSGYYVSRPFFKAVDRVLE+TLRAAEM+LALLL  CQRSQCEKLP
Sbjct: 481  LSGDFFTYADRQEDYWSGYYVSRPFFKAVDRVLERTLRAAEMMLALLLAPCQRSQCEKLP 540

Query: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600
            +GFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYG+RMHTSLQDLHIFMSK IEVLLGI
Sbjct: 541  LGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGIRMHTSLQDLHIFMSKAIEVLLGI 600

Query: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTREEVAMIIVNR 660
            RH+KSDQNPSQFEPEQ+RSKYDAQ VHK+IDLQEG YQSV+FFNPL QTREEVAM+IVNR
Sbjct: 601  RHDKSDQNPSQFEPEQMRSKYDAQPVHKSIDLQEGTYQSVIFFNPLEQTREEVAMVIVNR 660

Query: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720
            T+V VLDSNWTC+QSQISPEFQHDKA VFTGRHRIHWKISVPALGLQTYYIANGLFDCEK
Sbjct: 661  TEVTVLDSNWTCVQSQISPEFQHDKAKVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720

Query: 721  PKPAKLKFFSTSTSLPCATPYICSKADGDVAVIGNQHQSLVFHGKQGLLHKIINKNGPQN 780
            PKPAKLKFFS STSLPC TPY+CSKAD DVA I NQHQSLVF  K GLL K+INKNG +N
Sbjct: 721  PKPAKLKFFSKSTSLPCPTPYVCSKADSDVAEIENQHQSLVFDVKHGLLQKVINKNGSEN 780

Query: 781  VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840
            VVNEEIA+Y+SWGSGAYLFKP G+A+SI + GGLTVITEGPLMQEV+SYPKTGWE SPIS
Sbjct: 781  VVNEEIAMYSSWGSGAYLFKPTGEAKSITEEGGLTVITEGPLMQEVFSYPKTGWEPSPIS 840

Query: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900
            HSTRLYSGGN+IQEHLIEMEYHVELLG+E+DD+ELIVRYKTDIDNKRIFYSDLNG Q+SR
Sbjct: 841  HSTRLYSGGNSIQEHLIEMEYHVELLGREYDDRELIVRYKTDIDNKRIFYSDLNGLQMSR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960
            RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRL  DD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLYRDD 960

Query: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSLCIGARLNYPLHTFI 1020
            GRGLGQGV DNRAMNV+FHI LES+VSTKLNPVS+YSPLSPSLLS CIG  LNYPLH FI
Sbjct: 961  GRGLGQGVTDNRAMNVVFHILLESNVSTKLNPVSSYSPLSPSLLSHCIGGHLNYPLHAFI 1020

Query: 1021 GKKPQASSVQPSPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD 1080
             KKPQ SS+QP  RSFSPLAAPLPCDLHIVSFKVPRPLKY+QQSPEDPRFLLIFHRRHWD
Sbjct: 1021 AKKPQPSSLQPPSRSFSPLAAPLPCDLHIVSFKVPRPLKYTQQSPEDPRFLLIFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNCT+ +DEP NIFNMFKGLAVS ARA+SLNLLHEDTEMLGYNEQSGDV HE
Sbjct: 1081 SSYCKTARSNCTKVADEPFNIFNMFKGLAVSKARASSLNLLHEDTEMLGYNEQSGDVGHE 1140

Query: 1141 GQLHIPPMEIQAYKLELKPR 1161
            GQLHIPPME++AYKLELKPR
Sbjct: 1141 GQLHIPPMEVRAYKLELKPR 1160

BLAST of CmaCh14G006220 vs. ExPASy TrEMBL
Match: A0A1S3B8V9 (Alpha-mannosidase OS=Cucumis melo OX=3656 GN=LOC103487047 PE=3 SV=1)

HSP 1 Score: 2187.9 bits (5668), Expect = 0.0e+00
Identity = 1048/1160 (90.34%), Postives = 1101/1160 (94.91%), Query Frame = 0

Query: 1    MAFSSFTGGGGSRRGGWASSILPFSSASPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60
            MAFSSFTGG G+RRGGWA SILPFSS SPSAKPKHNRKYRRRLAIRDFIF+N FTIGLL+
Sbjct: 1    MAFSSFTGGSGARRGGWAGSILPFSSVSPSAKPKHNRKYRRRLAIRDFIFSNFFTIGLLI 60

Query: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRQRKPIVSENWNSEVLSSNVDITTKKLYDK 120
            SF FFFIVL+RYGVPKPISS  KSHA RSHR RKPIVSENWNSEVLSSNVDITTK+LYDK
Sbjct: 61   SFFFFFIVLLRYGVPKPISSPFKSHAVRSHRPRKPIVSENWNSEVLSSNVDITTKELYDK 120

Query: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180
            IEFLD+DGGPWKQGWRVTYKGDEWDSEKLK+FVVPHSHNDPGWKLTVDEYYE+QSRHIL+
Sbjct: 121  IEFLDIDGGPWKQGWRVTYKGDEWDSEKLKVFVVPHSHNDPGWKLTVDEYYERQSRHILD 180

Query: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240
            TIV+ALS+DSRRKFIWEEMSYLEKWWRDASDEKKESF  LVKNGQLEIVGGGWVMNDEAN
Sbjct: 181  TIVEALSRDSRRKFIWEEMSYLEKWWRDASDEKKESFAALVKNGQLEIVGGGWVMNDEAN 240

Query: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
            SHYFAIIEQMAEGN+WL ET+G+VPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNIWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELA HKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELALHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420
            S YELCPWRQDPVEINKENVQERA  LLDQYRKKSVLYRTNTLLIPLGDDFRY++IDEAE
Sbjct: 361  SLYELCPWRQDPVEINKENVQERATTLLDQYRKKSVLYRTNTLLIPLGDDFRYINIDEAE 420

Query: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFDYINS PSLNAEA FGTLEDYFRTLRDEAEKINYSLPGEVGSS + GFPS
Sbjct: 421  AQFKNYQLLFDYINSTPSLNAEANFGTLEDYFRTLRDEAEKINYSLPGEVGSSLVGGFPS 480

Query: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540
            LSGDFFTYADRQ+DYWSGYYVSRPFFKAVDRVLE+TLRAAEM+LALLL  CQRSQCEKLP
Sbjct: 481  LSGDFFTYADRQEDYWSGYYVSRPFFKAVDRVLERTLRAAEMMLALLLAPCQRSQCEKLP 540

Query: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600
            +GFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYG+RMHTSLQDLHIFMSK IEVLLGI
Sbjct: 541  LGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGIRMHTSLQDLHIFMSKAIEVLLGI 600

Query: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTREEVAMIIVNR 660
            RH+KSDQNPSQFEPEQ+RSKYDAQ VHK+IDLQEG YQSV+FFNPL QTREEVAM+IVNR
Sbjct: 601  RHDKSDQNPSQFEPEQMRSKYDAQPVHKSIDLQEGTYQSVIFFNPLEQTREEVAMVIVNR 660

Query: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720
            T+V VLDSNWTC+QSQISPEFQHDKA VFTGRHRIHWKISVPALGLQTYYIANGLFDCEK
Sbjct: 661  TEVTVLDSNWTCVQSQISPEFQHDKAKVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720

Query: 721  PKPAKLKFFSTSTSLPCATPYICSKADGDVAVIGNQHQSLVFHGKQGLLHKIINKNGPQN 780
            PKPAKLKFFS STSLPC TPY+CSKAD DVA I NQHQSLVF  K GLL K+INKNG +N
Sbjct: 721  PKPAKLKFFSKSTSLPCPTPYVCSKADSDVAEIENQHQSLVFDVKHGLLQKVINKNGSEN 780

Query: 781  VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840
            VVNEEIA+Y+SWGSGAYLFKP G+A+SI + GGLTVITEGPLMQEV+SYPKTGWE SPIS
Sbjct: 781  VVNEEIAMYSSWGSGAYLFKPTGEAKSITEEGGLTVITEGPLMQEVFSYPKTGWEPSPIS 840

Query: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900
            HSTRLYSGGN+IQEHLIEMEYHVELLG+E+DD+ELIVRYKTDIDNKRIFYSDLNG Q+SR
Sbjct: 841  HSTRLYSGGNSIQEHLIEMEYHVELLGREYDDRELIVRYKTDIDNKRIFYSDLNGLQMSR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960
            RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRL  DD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLYRDD 960

Query: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSLCIGARLNYPLHTFI 1020
            GRGLGQGV DNRAMNV+FHI LES+VSTKLNPVS+YSPLSPSLLS CIG  LNYPLH FI
Sbjct: 961  GRGLGQGVTDNRAMNVVFHILLESNVSTKLNPVSSYSPLSPSLLSHCIGGHLNYPLHAFI 1020

Query: 1021 GKKPQASSVQPSPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD 1080
             KKPQ SS+QP  RSFSPLAAPLPCDLHIVSFKVPRPLKY+QQSPEDPRFLLIFHRRHWD
Sbjct: 1021 AKKPQPSSLQPPSRSFSPLAAPLPCDLHIVSFKVPRPLKYTQQSPEDPRFLLIFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNCT+ +DEP NIFNMFKGLAVS ARA+SLNLLHEDTEMLGYNEQSGDV HE
Sbjct: 1081 SSYCKTARSNCTKAADEPFNIFNMFKGLAVSKARASSLNLLHEDTEMLGYNEQSGDVGHE 1140

Query: 1141 GQLHIPPMEIQAYKLELKPR 1161
            GQLHIPPME++AYKLELKPR
Sbjct: 1141 GQLHIPPMEVRAYKLELKPR 1160

BLAST of CmaCh14G006220 vs. ExPASy TrEMBL
Match: A0A0A0LFD6 (Alpha-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_3G829090 PE=3 SV=1)

HSP 1 Score: 2177.9 bits (5642), Expect = 0.0e+00
Identity = 1043/1160 (89.91%), Postives = 1101/1160 (94.91%), Query Frame = 0

Query: 1    MAFSSFTGGGGSRRGGWASSILPFSSASPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60
            MAFSSFTGG G+RRGGWA SILPFSS SPSAKPKHNRKYRRRLAIRDFIF+N FTIGLL+
Sbjct: 1    MAFSSFTGGSGARRGGWAGSILPFSSVSPSAKPKHNRKYRRRLAIRDFIFSNFFTIGLLI 60

Query: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRQRKPIVSENWNSEVLSSNVDITTKKLYDK 120
            SF FF IVL+RYGVPKPISS  KSHA RSHR RKPIVSENWNSEVLSSNVDITTK+LYD+
Sbjct: 61   SFFFFLIVLLRYGVPKPISSPFKSHAIRSHRPRKPIVSENWNSEVLSSNVDITTKELYDR 120

Query: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180
            IEFLD+DGGPWKQGW+VTYKG+EWDSEKLK+FVVPHSHNDPGWKLTVDEYY++QSRHIL+
Sbjct: 121  IEFLDIDGGPWKQGWKVTYKGNEWDSEKLKVFVVPHSHNDPGWKLTVDEYYDRQSRHILD 180

Query: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240
            TIV+ALS+DSRRKFIWEEMSYLEKWWRDASDEKKESF  LVKNGQLEIVGGGWVMNDEAN
Sbjct: 181  TIVEALSRDSRRKFIWEEMSYLEKWWRDASDEKKESFAALVKNGQLEIVGGGWVMNDEAN 240

Query: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
            SHYFAIIEQMAEGNMWL ET+G+VPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNMWLNETIGVVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELA HKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELALHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420
            S YELCPWRQDPVEINKENVQERA  LLDQYRKKSVLYRTNTLLIPLGDDFRY++IDEAE
Sbjct: 361  SLYELCPWRQDPVEINKENVQERATTLLDQYRKKSVLYRTNTLLIPLGDDFRYINIDEAE 420

Query: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFDYINSNPSLNAEA FGTLEDYFRTLRDEAEKINYSLPGEVGSS + GFPS
Sbjct: 421  AQFKNYQLLFDYINSNPSLNAEANFGTLEDYFRTLRDEAEKINYSLPGEVGSSLVGGFPS 480

Query: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540
            LSGDFFTYADRQ+DYWSGYYVSRPFFKAVDRVLE+TLRAAEM+LALLLG CQRSQCEKLP
Sbjct: 481  LSGDFFTYADRQEDYWSGYYVSRPFFKAVDRVLERTLRAAEMMLALLLGPCQRSQCEKLP 540

Query: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600
            +GFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSK IEVLLGI
Sbjct: 541  LGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKAIEVLLGI 600

Query: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTREEVAMIIVNR 660
            RH+KSDQNPSQFEPEQ+RSKYDAQ VHK+IDLQEG YQSV+FFNPL QTREEVAM+IVNR
Sbjct: 601  RHDKSDQNPSQFEPEQMRSKYDAQPVHKSIDLQEGTYQSVIFFNPLEQTREEVAMVIVNR 660

Query: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720
            T+V VLDSNWTC+QSQISPEFQHDKA VFTGRHRIHWKI VPALGLQTYYIANGLFDCEK
Sbjct: 661  TEVTVLDSNWTCVQSQISPEFQHDKAKVFTGRHRIHWKILVPALGLQTYYIANGLFDCEK 720

Query: 721  PKPAKLKFFSTSTSLPCATPYICSKADGDVAVIGNQHQSLVFHGKQGLLHKIINKNGPQN 780
            PKPAKLK FSTSTSLPC TPY CSK +GDVA I NQHQSLVF  K GLL K+INK+G QN
Sbjct: 721  PKPAKLKIFSTSTSLPCPTPYACSKVNGDVAEIENQHQSLVFDVKHGLLQKVINKDGSQN 780

Query: 781  VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840
             VNEEIA+Y+SWGSGAYLFKP G+A+SI + GGLTV+TEGPLMQEV+SYPKTGWE SPIS
Sbjct: 781  FVNEEIAMYSSWGSGAYLFKPTGEAKSITEEGGLTVVTEGPLMQEVFSYPKTGWEPSPIS 840

Query: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900
            HSTRLYSGGN+IQEHLIEMEYHVELLG+E+DD+ELIVRYKTDIDNKRIFYSDLNG Q+SR
Sbjct: 841  HSTRLYSGGNSIQEHLIEMEYHVELLGREYDDRELIVRYKTDIDNKRIFYSDLNGLQMSR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960
            RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRL  DD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLYRDD 960

Query: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSLCIGARLNYPLHTFI 1020
            GRGLGQGV DNRAMNV+FHI LES+VSTKLNPVS+YSPLSPSLLS CIGARLNYPLH FI
Sbjct: 961  GRGLGQGVTDNRAMNVVFHILLESNVSTKLNPVSSYSPLSPSLLSHCIGARLNYPLHAFI 1020

Query: 1021 GKKPQASSVQPSPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD 1080
             KKPQ SS+QP+ RSFSPLAAPLPCDLHIVSFKVPRPLKY+QQS EDPRFLLIFHRRHWD
Sbjct: 1021 AKKPQPSSLQPTSRSFSPLAAPLPCDLHIVSFKVPRPLKYTQQSLEDPRFLLIFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNC R +DEP N+FNMFKGLAVS+ARA+SLNLLHEDTEMLGYNEQSGDV HE
Sbjct: 1081 SSYCKTARSNCARVADEPFNLFNMFKGLAVSDARASSLNLLHEDTEMLGYNEQSGDVGHE 1140

Query: 1141 GQLHIPPMEIQAYKLELKPR 1161
            GQLHIPPME++AYKLELKPR
Sbjct: 1141 GQLHIPPMEVRAYKLELKPR 1160

BLAST of CmaCh14G006220 vs. NCBI nr
Match: XP_022983686.1 (alpha-mannosidase 2 [Cucurbita maxima])

HSP 1 Score: 2378.2 bits (6162), Expect = 0.0e+00
Identity = 1160/1160 (100.00%), Postives = 1160/1160 (100.00%), Query Frame = 0

Query: 1    MAFSSFTGGGGSRRGGWASSILPFSSASPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60
            MAFSSFTGGGGSRRGGWASSILPFSSASPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV
Sbjct: 1    MAFSSFTGGGGSRRGGWASSILPFSSASPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60

Query: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRQRKPIVSENWNSEVLSSNVDITTKKLYDK 120
            SFIFFFIVLIRYGVPKPISSHIKSHATRSHRQRKPIVSENWNSEVLSSNVDITTKKLYDK
Sbjct: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRQRKPIVSENWNSEVLSSNVDITTKKLYDK 120

Query: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180
            IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN
Sbjct: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180

Query: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240
            TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN
Sbjct: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240

Query: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
            SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420
            SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE
Sbjct: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420

Query: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS
Sbjct: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480

Query: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540
            LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP
Sbjct: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540

Query: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600
            VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI
Sbjct: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600

Query: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTREEVAMIIVNR 660
            RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTREEVAMIIVNR
Sbjct: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTREEVAMIIVNR 660

Query: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720
            TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK
Sbjct: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720

Query: 721  PKPAKLKFFSTSTSLPCATPYICSKADGDVAVIGNQHQSLVFHGKQGLLHKIINKNGPQN 780
            PKPAKLKFFSTSTSLPCATPYICSKADGDVAVIGNQHQSLVFHGKQGLLHKIINKNGPQN
Sbjct: 721  PKPAKLKFFSTSTSLPCATPYICSKADGDVAVIGNQHQSLVFHGKQGLLHKIINKNGPQN 780

Query: 781  VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840
            VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS
Sbjct: 781  VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840

Query: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900
            HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR
Sbjct: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960
            RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960

Query: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSLCIGARLNYPLHTFI 1020
            GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSLCIGARLNYPLHTFI
Sbjct: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSLCIGARLNYPLHTFI 1020

Query: 1021 GKKPQASSVQPSPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD 1080
            GKKPQASSVQPSPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD
Sbjct: 1021 GKKPQASSVQPSPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE
Sbjct: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140

Query: 1141 GQLHIPPMEIQAYKLELKPR 1161
            GQLHIPPMEIQAYKLELKPR
Sbjct: 1141 GQLHIPPMEIQAYKLELKPR 1160

BLAST of CmaCh14G006220 vs. NCBI nr
Match: KAG6580996.1 (Alpha-mannosidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2343.9 bits (6073), Expect = 0.0e+00
Identity = 1143/1160 (98.53%), Postives = 1146/1160 (98.79%), Query Frame = 0

Query: 1    MAFSSFTGGGGSRRGGWASSILPFSSASPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60
            MAFSSFTGGGGSRRGGWASSILPFSS SPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV
Sbjct: 1    MAFSSFTGGGGSRRGGWASSILPFSSVSPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60

Query: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRQRKPIVSENWNSEVLSSNVDITTKKLYDK 120
            SFIFFFIVLIRYGVPKPISSHIKSHATRSHR RKPIVSENWNSEVLSS VDITTKKLYDK
Sbjct: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRPRKPIVSENWNSEVLSSTVDITTKKLYDK 120

Query: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180
            IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN
Sbjct: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180

Query: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240
            TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN
Sbjct: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240

Query: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
            SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420
            SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE
Sbjct: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420

Query: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQI GFPS
Sbjct: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQIGGFPS 480

Query: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540
            LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP
Sbjct: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540

Query: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600
            VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI
Sbjct: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600

Query: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTREEVAMIIVNR 660
            RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEG YQSVVFFNPL QTREEVAMIIVNR
Sbjct: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGTYQSVVFFNPLEQTREEVAMIIVNR 660

Query: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720
            TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHR+HWKISVPALGLQTYYIANGLFDCEK
Sbjct: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRLHWKISVPALGLQTYYIANGLFDCEK 720

Query: 721  PKPAKLKFFSTSTSLPCATPYICSKADGDVAVIGNQHQSLVFHGKQGLLHKIINKNGPQN 780
            PKPAKLKFFSTSTSLPCATPYICSKAD DVAVI NQHQSLVF GK GLLHKIINKNG QN
Sbjct: 721  PKPAKLKFFSTSTSLPCATPYICSKADDDVAVIENQHQSLVFDGKHGLLHKIINKNGSQN 780

Query: 781  VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840
            VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS
Sbjct: 781  VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840

Query: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900
            HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR
Sbjct: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960
            RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960

Query: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSLCIGARLNYPLHTFI 1020
            GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLS CIGARLNYPLHTFI
Sbjct: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSHCIGARLNYPLHTFI 1020

Query: 1021 GKKPQASSVQPSPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD 1080
             KKPQASSVQP PRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLL+FHRRHWD
Sbjct: 1021 AKKPQASSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLVFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE
Sbjct: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140

Query: 1141 GQLHIPPMEIQAYKLELKPR 1161
            GQLHIPPME+QAYKLELKPR
Sbjct: 1141 GQLHIPPMEVQAYKLELKPR 1160

BLAST of CmaCh14G006220 vs. NCBI nr
Match: KAG7017738.1 (Alpha-mannosidase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2341.6 bits (6067), Expect = 0.0e+00
Identity = 1142/1160 (98.45%), Postives = 1145/1160 (98.71%), Query Frame = 0

Query: 1    MAFSSFTGGGGSRRGGWASSILPFSSASPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60
            MAFSSFTGGGGSRRGGWASSILPFSS SPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV
Sbjct: 1    MAFSSFTGGGGSRRGGWASSILPFSSVSPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60

Query: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRQRKPIVSENWNSEVLSSNVDITTKKLYDK 120
            SFIFFFIVLIRYGVPKPISSHIKSHATRSHR RKPIVSENWNSEVLSS VDITTKKLYDK
Sbjct: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRPRKPIVSENWNSEVLSSTVDITTKKLYDK 120

Query: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180
            IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN
Sbjct: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180

Query: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240
            TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN
Sbjct: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240

Query: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
            SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420
            SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE
Sbjct: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420

Query: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQI GFPS
Sbjct: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQIGGFPS 480

Query: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540
            LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP
Sbjct: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540

Query: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600
            VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI
Sbjct: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600

Query: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTREEVAMIIVNR 660
            RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEG YQSVVFFNPL QTREEVAMIIVNR
Sbjct: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGTYQSVVFFNPLEQTREEVAMIIVNR 660

Query: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720
            TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHR+HWKISVPALGLQTYYIANGLFDCEK
Sbjct: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRLHWKISVPALGLQTYYIANGLFDCEK 720

Query: 721  PKPAKLKFFSTSTSLPCATPYICSKADGDVAVIGNQHQSLVFHGKQGLLHKIINKNGPQN 780
            PKPAKLKFFSTSTSLPCATPYICSKAD DVAVI NQHQSLVF GK GLLHKIINKNG QN
Sbjct: 721  PKPAKLKFFSTSTSLPCATPYICSKADDDVAVIENQHQSLVFDGKHGLLHKIINKNGSQN 780

Query: 781  VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840
            VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS
Sbjct: 781  VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840

Query: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900
            HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR
Sbjct: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960
            RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960

Query: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSLCIGARLNYPLHTFI 1020
            GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLS CI ARLNYPLHTFI
Sbjct: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSHCISARLNYPLHTFI 1020

Query: 1021 GKKPQASSVQPSPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD 1080
             KKPQASSVQP PRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLL+FHRRHWD
Sbjct: 1021 AKKPQASSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLVFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE
Sbjct: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140

Query: 1141 GQLHIPPMEIQAYKLELKPR 1161
            GQLHIPPME+QAYKLELKPR
Sbjct: 1141 GQLHIPPMEVQAYKLELKPR 1160

BLAST of CmaCh14G006220 vs. NCBI nr
Match: XP_023526938.1 (alpha-mannosidase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2338.5 bits (6059), Expect = 0.0e+00
Identity = 1142/1160 (98.45%), Postives = 1144/1160 (98.62%), Query Frame = 0

Query: 1    MAFSSFTGGGGSRRGGWASSILPFSSASPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60
            MAFSSFTGGGGSRRGGWASSILPFSS SPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV
Sbjct: 1    MAFSSFTGGGGSRRGGWASSILPFSSVSPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60

Query: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRQRKPIVSENWNSEVLSSNVDITTKKLYDK 120
            SFIFFFIVLIRYGVPKPISSHIKSHATRSHR RKPIVSENWN+EVLSS VDITTKKLYDK
Sbjct: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRPRKPIVSENWNNEVLSSTVDITTKKLYDK 120

Query: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180
            IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN
Sbjct: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180

Query: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240
            TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN
Sbjct: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240

Query: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
            SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420
            SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE
Sbjct: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420

Query: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQI GFPS
Sbjct: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQIGGFPS 480

Query: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540
            LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP
Sbjct: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540

Query: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600
            VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI
Sbjct: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600

Query: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTREEVAMIIVNR 660
            RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDL EG YQSVVFFNPL QTREEVAMIIVNR
Sbjct: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLIEGTYQSVVFFNPLEQTREEVAMIIVNR 660

Query: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720
            TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK
Sbjct: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720

Query: 721  PKPAKLKFFSTSTSLPCATPYICSKADGDVAVIGNQHQSLVFHGKQGLLHKIINKNGPQN 780
            PKPAKLKFFSTSTSLPCATPYICSKAD DVAVI NQHQSLVF GK GLLHKIINKNG QN
Sbjct: 721  PKPAKLKFFSTSTSLPCATPYICSKADDDVAVIENQHQSLVFDGKHGLLHKIINKNGSQN 780

Query: 781  VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840
            VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS
Sbjct: 781  VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840

Query: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900
            HSTRLYSGGN IQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR
Sbjct: 841  HSTRLYSGGNPIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960
            RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960

Query: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSLCIGARLNYPLHTFI 1020
            GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLS CIGARLNYPLHTFI
Sbjct: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSHCIGARLNYPLHTFI 1020

Query: 1021 GKKPQASSVQPSPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD 1080
             KKPQASSVQP PRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD
Sbjct: 1021 AKKPQASSVQPPPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE
Sbjct: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140

Query: 1141 GQLHIPPMEIQAYKLELKPR 1161
            GQLHIPPME+QAYKLELKPR
Sbjct: 1141 GQLHIPPMEVQAYKLELKPR 1160

BLAST of CmaCh14G006220 vs. NCBI nr
Match: XP_022934236.1 (alpha-mannosidase 2 [Cucurbita moschata])

HSP 1 Score: 2334.7 bits (6049), Expect = 0.0e+00
Identity = 1140/1160 (98.28%), Postives = 1144/1160 (98.62%), Query Frame = 0

Query: 1    MAFSSFTGGGGSRRGGWASSILPFSSASPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60
            MAFSSFTGGGGSRRGGWASSILPFSS SPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV
Sbjct: 1    MAFSSFTGGGGSRRGGWASSILPFSSVSPSAKPKHNRKYRRRLAIRDFIFANIFTIGLLV 60

Query: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRQRKPIVSENWNSEVLSSNVDITTKKLYDK 120
            SFIFFFIVLIRYGVPKPISSHIKSHATRSHR RKPIVS+NWNSEVLSS VDITTKKLYDK
Sbjct: 61   SFIFFFIVLIRYGVPKPISSHIKSHATRSHRPRKPIVSKNWNSEVLSSTVDITTKKLYDK 120

Query: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180
            IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN
Sbjct: 121  IEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYEKQSRHILN 180

Query: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240
            TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN
Sbjct: 181  TIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEAN 240

Query: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300
            SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE
Sbjct: 241  SHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENMLIQRTHYE 300

Query: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360
            LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG
Sbjct: 301  LKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRG 360

Query: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420
            SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE
Sbjct: 361  SSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAE 420

Query: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPS 480
            AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQI GFPS
Sbjct: 421  AQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQIGGFPS 480

Query: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540
            LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP
Sbjct: 481  LSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLP 540

Query: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600
            VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI
Sbjct: 541  VGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGI 600

Query: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTREEVAMIIVNR 660
            RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEG YQSVVFFNPL QTREEVAMIIVNR
Sbjct: 601  RHEKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGTYQSVVFFNPLEQTREEVAMIIVNR 660

Query: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEK 720
            TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHR+HWKISVPALGLQTYYIANGLFDCEK
Sbjct: 661  TDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRLHWKISVPALGLQTYYIANGLFDCEK 720

Query: 721  PKPAKLKFFSTSTSLPCATPYICSKADGDVAVIGNQHQSLVFHGKQGLLHKIINKNGPQN 780
            PKPAKLKFFSTSTSLPCATPYICSKAD DVAVI NQHQSLVF GK GLLHKIINKNG QN
Sbjct: 721  PKPAKLKFFSTSTSLPCATPYICSKADDDVAVIENQHQSLVFDGKHGLLHKIINKNGSQN 780

Query: 781  VVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840
            VV+EEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS
Sbjct: 781  VVSEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYPKTGWEKSPIS 840

Query: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900
            HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR
Sbjct: 841  HSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFYSDLNGFQISR 900

Query: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960
            RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD
Sbjct: 901  RESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEIMLDRRLSMDD 960

Query: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSLCIGARLNYPLHTFI 1020
            GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLS  IGARLNYPLHTFI
Sbjct: 961  GRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSHGIGARLNYPLHTFI 1020

Query: 1021 GKKPQASSVQPSPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD 1080
             KKPQASSVQP PRSFSPLA PLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD
Sbjct: 1021 AKKPQASSVQPPPRSFSPLATPLPCDLHIVSFKVPRPLKYSQQSPEDPRFLLIFHRRHWD 1080

Query: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140
            SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE
Sbjct: 1081 SSYCKTARSNCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEMLGYNEQSGDVAHE 1140

Query: 1141 GQLHIPPMEIQAYKLELKPR 1161
            GQLHIPPME+QAYKLELKPR
Sbjct: 1141 GQLHIPPMEVQAYKLELKPR 1160

BLAST of CmaCh14G006220 vs. TAIR 10
Match: AT5G14950.1 (golgi alpha-mannosidase II )

HSP 1 Score: 1697.2 bits (4394), Expect = 0.0e+00
Identity = 829/1176 (70.49%), Postives = 962/1176 (81.80%), Query Frame = 0

Query: 1    MAFSSFTG-------GGGSRRGGWASSILPFSSASPSAKPKHNRKYRRRLAIRDFIFANI 60
            M FSS+ G       GGG+  GGW  S+LP  +A   +K   NRK R+R  + +FIFAN 
Sbjct: 1    MPFSSYIGNSRRSSTGGGT--GGWGQSLLP--TALSKSKLAINRKPRKRTLVVNFIFANF 60

Query: 61   FTIGLLVSFIFFFIVLIRYGVPKPISSH-IKSHATRSHRQRKPIVSENWNSEVLSSNVDI 120
            F I L VS +FF + L  +GVP PISS  + S + R  + RK I     N     + VDI
Sbjct: 61   FVIALTVSLLFFLLTLFHFGVPGPISSRFLTSRSNRIVKPRKNINRRPLNDSNSGAVVDI 120

Query: 121  TTKKLYDKIEFLDVDGGPWKQGWRVTYKGDEWDSEKLKIFVVPHSHNDPGWKLTVDEYYE 180
            TTK LYD+IEFLD DGGPWKQGWRVTYK DEW+ EKLKIFVVPHSHNDPGWKLTV+EYY+
Sbjct: 121  TTKDLYDRIEFLDTDGGPWKQGWRVTYKDDEWEKEKLKIFVVPHSHNDPGWKLTVEEYYQ 180

Query: 181  KQSRHILNTIVDALSQDSRRKFIWEEMSYLEKWWRDASDEKKESFVTLVKNGQLEIVGGG 240
            +QSRHIL+TIV+ LS+DSRRKFIWEEMSYLE+WWRDAS  K+E+   LVK+GQLEIVGGG
Sbjct: 181  RQSRHILDTIVETLSKDSRRKFIWEEMSYLERWWRDASPNKQEALTKLVKDGQLEIVGGG 240

Query: 241  WVMNDEANSHYFAIIEQMAEGNMWLKETVGIVPKNSWAIDPFGYSPTMAYLLRRMGFENM 300
            WVMNDEANSHYFAIIEQ+AEGNMWL +T+G++PKNSWAIDPFGYS TMAYLLRRMGFENM
Sbjct: 241  WVMNDEANSHYFAIIEQIAEGNMWLNDTIGVIPKNSWAIDPFGYSSTMAYLLRRMGFENM 300

Query: 301  LIQRTHYELKKELAQHKNLEFIWRQSWDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQ 360
            LIQRTHYELKK+LAQHKNLE+IWRQSWDA ETTDIFVHMMPFYSYDIPHTCGPEPAICCQ
Sbjct: 301  LIQRTHYELKKDLAQHKNLEYIWRQSWDAMETTDIFVHMMPFYSYDIPHTCGPEPAICCQ 360

Query: 361  FDFARSRGSSYELCPWRQDPVEINKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFR 420
            FDFAR RG  YELCPW + PVE   ENVQERA+ LLDQYRKKS LYRTNTLLIPLGDDFR
Sbjct: 361  FDFARMRGFKYELCPWGKHPVETTLENVQERALKLLDQYRKKSTLYRTNTLLIPLGDDFR 420

Query: 421  YVSIDEAEAQFKNYQLLFDYINSNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGS 480
            Y+SIDEAEAQF+NYQ+LFD+INSNPSLNAEAKFGTLEDYFRT+R+EA+++NYS PGEVGS
Sbjct: 421  YISIDEAEAQFRNYQMLFDHINSNPSLNAEAKFGTLEDYFRTVREEADRVNYSRPGEVGS 480

Query: 481  SQISGFPSLSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQ 540
             Q+ GFPSLSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLE TLR AE++++ LLG C 
Sbjct: 481  GQVVGFPSLSGDFFTYADRQQDYWSGYYVSRPFFKAVDRVLEHTLRGAEIMMSFLLGYCH 540

Query: 541  RSQCEKLPVGFSYKLTAARRNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSK 600
            R QCEK P  F+YKLTAARRNLALFQHHDGVTGTAKD+VV+DYG RMHTSLQDL IFMSK
Sbjct: 541  RIQCEKFPTSFTYKLTAARRNLALFQHHDGVTGTAKDYVVQDYGTRMHTSLQDLQIFMSK 600

Query: 601  GIEVLLGIRH--EKSDQNPSQFEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTR 660
             IEVLLGIRH  EKSDQ+PS FE EQ+RSKYDA+ VHK I  +EG   +V+ FNP  QTR
Sbjct: 601  AIEVLLGIRHEKEKSDQSPSFFEAEQMRSKYDARPVHKPIAAREGNSHTVILFNPSEQTR 660

Query: 661  EEVAMIIVNRTDVAVLDSNWTCLQSQISPEFQHDKANVFTGRHRIHWKISVPALGLQTYY 720
            EEV  ++VNR +++VLDSNWTC+ SQISPE QHD   +FTGRHR++WK S+PALGL+TY+
Sbjct: 661  EEVVTVVVNRAEISVLDSNWTCVPSQISPEVQHDDTKLFTGRHRLYWKASIPALGLRTYF 720

Query: 721  IANGLFDCEKPKPAKLKFFSTSTSLPCATPYICSKADGDVAVIGNQHQSLVFHGKQGLLH 780
            IANG  +CEK  P+KLK+ S     PC  PY CSK D DV  I N+HQ+LVF  K G L 
Sbjct: 721  IANGNVECEKATPSKLKYASEFDPFPCPPPYSCSKLDNDVTEIRNEHQTLVFDVKNGSLR 780

Query: 781  KIINKNGPQNVVNEEIALYTSWGSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVYSYP 840
            KI+++NG + VV EEI +Y+S  SGAYLFKP+G+A+ I++  G  V +EG L+QEV+SYP
Sbjct: 781  KIVHRNGSETVVGEEIGMYSSPESGAYLFKPDGEAQPIVQPDGHVVTSEGLLVQEVFSYP 840

Query: 841  KTGWEKSPISHSTRLYSGGNTIQEHLIEMEYHVELLGKEFDDKELIVRYKTDIDNKRIFY 900
            KT WEKSP+S  TRLY+GGNT+Q+ ++E+EYHVELLG +FDD+ELIVRYKTD+DNK++FY
Sbjct: 841  KTKWEKSPLSQKTRLYTGGNTLQDQVVEIEYHVELLGNDFDDRELIVRYKTDVDNKKVFY 900

Query: 901  SDLNGFQISRRESYDKIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQSLGVASLKDGWLEI 960
            SDLNGFQ+SRRE+YDKIPLQGNYYPMPSLAF++GSNGQRFSVHSRQSLGVASLK+GWLEI
Sbjct: 901  SDLNGFQMSRRETYDKIPLQGNYYPMPSLAFIQGSNGQRFSVHSRQSLGVASLKEGWLEI 960

Query: 961  MLDRRLSMDDGRGLGQGVRDNRAMNVIFHISLESSVSTKLNPVSNYSPLSPSLLSLCIGA 1020
            MLDRRL  DDGRGLGQGV DNRAM V+FH+  ES++S + +P SN +P +PSLLS  IGA
Sbjct: 961  MLDRRLVRDDGRGLGQGVMDNRAMTVVFHLLAESNIS-QADPASNTNPRNPSLLSHLIGA 1020

Query: 1021 RLNYPLHTFIGKKPQASSVQ-PSPRSFSPLAAPLPCDLHIVSFKVPRPLKYSQQSPED-P 1080
             LNYP++TFI KKPQ  SV+ P   SF+PLA PLPCDLHIV+FKVPRP KYSQQ  ED P
Sbjct: 1021 HLNYPINTFIAKKPQDISVRVPQYGSFAPLAKPLPCDLHIVNFKVPRPSKYSQQLEEDKP 1080

Query: 1081 RFLLIFHRRHWDSSYCKTARS-NCTRFSDEPVNIFNMFKGLAVSNARATSLNLLHEDTEM 1140
            RF LI +RR WDS+YC   R  NCT  ++EPVN  +MFK LA S  + TSLNLL ED E+
Sbjct: 1081 RFALILNRRAWDSAYCHKGRQVNCTSMANEPVNFSDMFKDLAASKVKPTSLNLLQEDMEI 1140

Query: 1141 LGYNEQ----SGDVAHEGQLHIPPMEIQAYKLELKP 1160
            LGY++Q          EG++ I PMEI+AYKLEL+P
Sbjct: 1141 LGYDDQELPRDSSQPREGRVSISPMEIRAYKLELRP 1171

BLAST of CmaCh14G006220 vs. TAIR 10
Match: AT5G66150.1 (Glycosyl hydrolase family 38 protein )

HSP 1 Score: 312.0 bits (798), Expect = 2.0e-84
Identity = 251/887 (28.30%), Postives = 401/887 (45.21%), Query Frame = 0

Query: 148 KLKIFVVPHSHNDPGWKLTVDEYYEKQS--------RHILNTIVDALSQDSRRKFIWEEM 207
           KL + +VPHSH+D GW  TVD+YY   +        R++L+++VD+L +D  RKF++ EM
Sbjct: 46  KLNVHLVPHSHDDVGWLKTVDQYYVGSNNRIQNACVRNVLDSVVDSLLRDPNRKFVFAEM 105

Query: 208 SYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEANSHYFAIIEQMAEGNMWLKE 267
           ++  +WW + S E++E    LVK+GQLE V GGW MNDEA  HY  +I+Q  +G+ ++K+
Sbjct: 106 AFFTRWWEEQSPERQEQVRRLVKSGQLEFVNGGWAMNDEATCHYIDMIDQTTKGHRFIKQ 165

Query: 268 TVGIVPKNSWAIDPFGYSPTMAYLL-RRMGFENMLIQRTHYELKKELAQHKNLEFIWRQS 327
                P+ +W IDPFG+S   AYLL   +G +++   R  Y+ +++    K+LE IWR S
Sbjct: 166 QFNTTPRAAWQIDPFGHSSVQAYLLGAELGLDSVHFARIDYQDREKRKAEKSLEVIWRGS 225

Query: 328 WDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRGSSYEL----CPWRQDPVE 387
              + ++ IF ++  F  Y  P                   G  YE+     P + +P  
Sbjct: 226 KTLDSSSQIFTNIF-FVHYGPP------------------TGFHYEVTDDYVPLQDNP-R 285

Query: 388 INKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAEAQFKNYQLLFDYIN 447
            +  N++E     ++     + + R N ++  +GDDF+Y     AE+ F+    L  Y+N
Sbjct: 286 FDGYNIKEAVNDFVNASLVYANVSRGNHVMWTMGDDFQY---QFAESWFRQMDRLIHYVN 345

Query: 448 SNPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPG-EVGSSQISG--FPSLSGDFFTYADR 507
            +  +NA                      YS P   V +  ++   +P  + DFF YADR
Sbjct: 346 KDGRVNA---------------------LYSTPSLYVDAKNVANVTWPLKTHDFFPYADR 405

Query: 508 QQDYWSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLPVGFSYKLTAAR 567
              YW+GY+ SRP  K   R L     AA   L  L+G              +Y L  A 
Sbjct: 406 AYAYWTGYFTSRPALKRYVRALSGYYMAARQ-LEFLVGKNSGGP-------NTYSLGDA- 465

Query: 568 RNLALFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGIRHEKSDQNPSQ 627
             L + QHHD VTGTAK HV  DY  R+     +    ++  +  L+    +     P+ 
Sbjct: 466 --LGIAQHHDAVTGTAKQHVTNDYMKRLALGASEAEAVVNSALACLMNKAPKGGCTKPAI 525

Query: 628 FEPEQVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTREEVAMIIVNRTDVAVLDSNWT 687
              +Q      + C      L       +V +N L   R E+  I VN   ++V DS+  
Sbjct: 526 AFSQQCSLMNISYCPSTEETLPGQKSLILVAYNSLGWNRTEIIRIPVNDAGLSVEDSSGN 585

Query: 688 CLQSQISPEFQHDKANVFTGRHRIHWK--ISVPALGLQTYYIANGLFDCEKPKPAKLKFF 747
            L +Q  P       NV +     + K  + + +L    Y++   +F  + P      FF
Sbjct: 586 TLDAQYIP-----MDNVTSNLRSFYTKAYLGISSLQRPKYWL---VFKAKVPPLGWNTFF 645

Query: 748 STSTSLPCATPY----ICSKADGDVAVIGNQHQSLVFHGKQGLLHKIIN-KNGPQNVVNE 807
            +  S   +  +    +      +   IG  +  +VF    G L ++ N + G    V++
Sbjct: 646 ISKASAQGSNNHKHSSVMLSPMNNTTEIGPGNLKMVFSSDSGRLERMYNSRTGADIKVDQ 705

Query: 808 EIALYTS--------WGSGAYLFKPNGDAESIIKAGGLTVIT----------------EG 867
               Y S          SGAY+F+PNG     + +  +  +T                 G
Sbjct: 706 NYFWYASNVGDAKDPQVSGAYIFRPNGSLAYPVSSSKICTVTSAFIGNGNVQSKLQIVRG 765

Query: 868 PLMQEVYSYPKTGWEKSP-ISHSTRLYSGGNTIQEHLIEMEYHVE--LLGK-EFDDKELI 927
           PL+ EV+       + SP ++   RLY      +EH  E E+ +    +GK     KE+I
Sbjct: 766 PLIDEVHQ------QFSPWVAQVVRLYKE----KEH-AEFEFTIGPISVGKGHLTGKEII 825

Query: 928 VRYKTDIDNKRIFYSDLNGFQISRRESYDKI--------PLQGNYYPMPSLAFMEGSNGQ 976
            R  TD+   + FY+D NG    +R   ++         P+ GNYYP+    +++    +
Sbjct: 826 TRMVTDMTTAKEFYTDSNGRDFLKRVRDNRTDWHLEVNEPIAGNYYPLNLGMYIKDEKAE 857

BLAST of CmaCh14G006220 vs. TAIR 10
Match: AT5G13980.1 (Glycosyl hydrolase family 38 protein )

HSP 1 Score: 309.3 bits (791), Expect = 1.3e-83
Identity = 243/874 (27.80%), Postives = 399/874 (45.65%), Query Frame = 0

Query: 148 KLKIFVVPHSHNDPGWKLTVDEYYEKQS--------RHILNTIVDALSQDSRRKFIWEEM 207
           KL + VVPHSH+D GW  TVD+YY   +        +++L++IV AL  D  RKFI+ E 
Sbjct: 36  KLNVHVVPHSHDDVGWLKTVDQYYVGSNNSIQVACVQNVLDSIVPALLADKNRKFIYVEQ 95

Query: 208 SYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEANSHYFAIIEQMAEGNMWLKE 267
           ++ ++WW + S+E K     L+ +GQLE++ GG  M+DEA  HY  +I+Q   G+ ++  
Sbjct: 96  AFFQRWWNEQSEEIKRIVKELIHSGQLELINGGMCMHDEAAPHYIDMIDQTTLGHRFIIR 155

Query: 268 TVGIVPKNSWAIDPFGYSPTMAYLL-RRMGFENMLIQRTHYELKKELAQHKNLEFIWRQS 327
              + P+  W IDPFG+S   AYLL   +GF+++   R  Y+ +++  + K LE IWR S
Sbjct: 156 EFNVTPRIGWQIDPFGHSAVQAYLLGAEVGFDSVFFGRIDYQDREKRYKEKTLEVIWRGS 215

Query: 328 WDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRGSSYEL---CPWRQDPVEI 387
                ++ IF    P  +Y+      P P            G  YE+    P  QD  ++
Sbjct: 216 KSLGSSSQIFAGAFP-TNYE------PPPG-----------GFYYEITDDSPVVQDDPDL 275

Query: 388 NKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAEAQFKNYQLLFDYINS 447
              NVQER    +     ++ + R N ++  +G DFRY     A   ++    L  Y+N 
Sbjct: 276 FDYNVQERVNAFVAAALDQANITRINHIMFTMGTDFRY---QYAHTWYRQMDKLIHYVNL 335

Query: 448 NPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPSLSGDFFTYADRQQDY 507
           +  +NA   F +    +   +  A +                +P  + D+F YADR   Y
Sbjct: 336 DGRVNA---FYSTPSIYTDAKHAANE---------------AWPLKTEDYFPYADRINAY 395

Query: 508 WSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLPVGFSYKLTAARRNLA 567
           W+GY+ SRP  K   RV+     AA   L    G  Q+               +    LA
Sbjct: 396 WTGYFTSRPALKRYVRVMSAYYLAARQ-LEFFKGRSQKGP----------NTDSLADALA 455

Query: 568 LFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGIRHEKSDQNPSQFEPE 627
           + QHHD V+GT+K HV  DY  R+     +    ++  +  L  +       NP+  +  
Sbjct: 456 IAQHHDAVSGTSKQHVANDYAKRLAIGYVEAESVVATSLAHLTKV---DPTLNPTFQQCL 515

Query: 628 QVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTREEVAMIIVNRTDVAVLDSNWTCLQS 687
            +   Y   C    ++L +G    V+ +NPL   R ++  + V   DV+V DS    ++S
Sbjct: 516 LLNISY---CPSSEVNLSDGKSLIVLAYNPLGWKRVDIVRLPVVGGDVSVHDSEGHEVES 575

Query: 688 QISP-------------EFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEKPK 747
           Q+ P             E    ++     ++ + + ++VP LG  TY I+          
Sbjct: 576 QLVPFTDEYVALRKYHVEAYLGQSPTQVPKYWLVFSVTVPPLGFTTYTISTA-------- 635

Query: 748 PAKLKFFSTSTSLPCATPYICSKADGDVAV--IGNQHQSLVFHGKQGLLHKIIN-KNGPQ 807
             K   +S+ +       Y+ +   G+ ++  IG+ H  L F   QG     +N +    
Sbjct: 636 -KKTDGYSSKS-------YVSNILKGEQSIINIGHGHLKLSFSTDQGTAINYVNGRTSMT 695

Query: 808 NVVNEEIALYTSW------------GSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVY 867
             V +  + Y+++             SGAY+F+PNG      +      +  GPL+ EV+
Sbjct: 696 EPVKQTFSYYSAYNGSNDKEPLIPQNSGAYVFRPNGTFPINPEGQVPLTVIHGPLVDEVH 755

Query: 868 SYPKTGWEKSPISHSTRLYSGGNTIQEHLIEMEYHVELLGKEFDD---KELIVRYKTDID 927
                 W    IS  TR+Y G    +EH +E+E+ V  +    DD   KE++ +  + + 
Sbjct: 756 QQINP-W----ISQITRVYKG----KEH-VEVEFIVGNI--PIDDGIGKEVVTQISSSLK 815

Query: 928 NKRIFYSDLNGFQ-ISRRESYD-------KIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQ 971
           + + FY+D +G   I R   Y          P+ GNYYP+    +++ S  + FSV   +
Sbjct: 816 SNKTFYTDSSGRDYIKRIRDYRSDWKLDVNQPIAGNYYPINHGIYLQDSK-KEFSVMVDR 824

BLAST of CmaCh14G006220 vs. TAIR 10
Match: AT5G13980.2 (Glycosyl hydrolase family 38 protein )

HSP 1 Score: 309.3 bits (791), Expect = 1.3e-83
Identity = 243/874 (27.80%), Postives = 399/874 (45.65%), Query Frame = 0

Query: 148 KLKIFVVPHSHNDPGWKLTVDEYYEKQS--------RHILNTIVDALSQDSRRKFIWEEM 207
           KL + VVPHSH+D GW  TVD+YY   +        +++L++IV AL  D  RKFI+ E 
Sbjct: 36  KLNVHVVPHSHDDVGWLKTVDQYYVGSNNSIQVACVQNVLDSIVPALLADKNRKFIYVEQ 95

Query: 208 SYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEANSHYFAIIEQMAEGNMWLKE 267
           ++ ++WW + S+E K     L+ +GQLE++ GG  M+DEA  HY  +I+Q   G+ ++  
Sbjct: 96  AFFQRWWNEQSEEIKRIVKELIHSGQLELINGGMCMHDEAAPHYIDMIDQTTLGHRFIIR 155

Query: 268 TVGIVPKNSWAIDPFGYSPTMAYLL-RRMGFENMLIQRTHYELKKELAQHKNLEFIWRQS 327
              + P+  W IDPFG+S   AYLL   +GF+++   R  Y+ +++  + K LE IWR S
Sbjct: 156 EFNVTPRIGWQIDPFGHSAVQAYLLGAEVGFDSVFFGRIDYQDREKRYKEKTLEVIWRGS 215

Query: 328 WDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRGSSYEL---CPWRQDPVEI 387
                ++ IF    P  +Y+      P P            G  YE+    P  QD  ++
Sbjct: 216 KSLGSSSQIFAGAFP-TNYE------PPPG-----------GFYYEITDDSPVVQDDPDL 275

Query: 388 NKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAEAQFKNYQLLFDYINS 447
              NVQER    +     ++ + R N ++  +G DFRY     A   ++    L  Y+N 
Sbjct: 276 FDYNVQERVNAFVAAALDQANITRINHIMFTMGTDFRY---QYAHTWYRQMDKLIHYVNL 335

Query: 448 NPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPSLSGDFFTYADRQQDY 507
           +  +NA   F +    +   +  A +                +P  + D+F YADR   Y
Sbjct: 336 DGRVNA---FYSTPSIYTDAKHAANE---------------AWPLKTEDYFPYADRINAY 395

Query: 508 WSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLPVGFSYKLTAARRNLA 567
           W+GY+ SRP  K   RV+     AA   L    G  Q+               +    LA
Sbjct: 396 WTGYFTSRPALKRYVRVMSAYYLAARQ-LEFFKGRSQKGP----------NTDSLADALA 455

Query: 568 LFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGIRHEKSDQNPSQFEPE 627
           + QHHD V+GT+K HV  DY  R+     +    ++  +  L  +       NP+  +  
Sbjct: 456 IAQHHDAVSGTSKQHVANDYAKRLAIGYVEAESVVATSLAHLTKV---DPTLNPTFQQCL 515

Query: 628 QVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTREEVAMIIVNRTDVAVLDSNWTCLQS 687
            +   Y   C    ++L +G    V+ +NPL   R ++  + V   DV+V DS    ++S
Sbjct: 516 LLNISY---CPSSEVNLSDGKSLIVLAYNPLGWKRVDIVRLPVVGGDVSVHDSEGHEVES 575

Query: 688 QISP-------------EFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEKPK 747
           Q+ P             E    ++     ++ + + ++VP LG  TY I+          
Sbjct: 576 QLVPFTDEYVALRKYHVEAYLGQSPTQVPKYWLVFSVTVPPLGFTTYTISTA-------- 635

Query: 748 PAKLKFFSTSTSLPCATPYICSKADGDVAV--IGNQHQSLVFHGKQGLLHKIIN-KNGPQ 807
             K   +S+ +       Y+ +   G+ ++  IG+ H  L F   QG     +N +    
Sbjct: 636 -KKTDGYSSKS-------YVSNILKGEQSIINIGHGHLKLSFSTDQGTAINYVNGRTSMT 695

Query: 808 NVVNEEIALYTSW------------GSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVY 867
             V +  + Y+++             SGAY+F+PNG      +      +  GPL+ EV+
Sbjct: 696 EPVKQTFSYYSAYNGSNDKEPLIPQNSGAYVFRPNGTFPINPEGQVPLTVIHGPLVDEVH 755

Query: 868 SYPKTGWEKSPISHSTRLYSGGNTIQEHLIEMEYHVELLGKEFDD---KELIVRYKTDID 927
                 W    IS  TR+Y G    +EH +E+E+ V  +    DD   KE++ +  + + 
Sbjct: 756 QQINP-W----ISQITRVYKG----KEH-VEVEFIVGNI--PIDDGIGKEVVTQISSSLK 815

Query: 928 NKRIFYSDLNGFQ-ISRRESYD-------KIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQ 971
           + + FY+D +G   I R   Y          P+ GNYYP+    +++ S  + FSV   +
Sbjct: 816 SNKTFYTDSSGRDYIKRIRDYRSDWKLDVNQPIAGNYYPINHGIYLQDSK-KEFSVMVDR 824

BLAST of CmaCh14G006220 vs. TAIR 10
Match: AT5G13980.3 (Glycosyl hydrolase family 38 protein )

HSP 1 Score: 309.3 bits (791), Expect = 1.3e-83
Identity = 243/874 (27.80%), Postives = 399/874 (45.65%), Query Frame = 0

Query: 148 KLKIFVVPHSHNDPGWKLTVDEYYEKQS--------RHILNTIVDALSQDSRRKFIWEEM 207
           KL + VVPHSH+D GW  TVD+YY   +        +++L++IV AL  D  RKFI+ E 
Sbjct: 36  KLNVHVVPHSHDDVGWLKTVDQYYVGSNNSIQVACVQNVLDSIVPALLADKNRKFIYVEQ 95

Query: 208 SYLEKWWRDASDEKKESFVTLVKNGQLEIVGGGWVMNDEANSHYFAIIEQMAEGNMWLKE 267
           ++ ++WW + S+E K     L+ +GQLE++ GG  M+DEA  HY  +I+Q   G+ ++  
Sbjct: 96  AFFQRWWNEQSEEIKRIVKELIHSGQLELINGGMCMHDEAAPHYIDMIDQTTLGHRFIIR 155

Query: 268 TVGIVPKNSWAIDPFGYSPTMAYLL-RRMGFENMLIQRTHYELKKELAQHKNLEFIWRQS 327
              + P+  W IDPFG+S   AYLL   +GF+++   R  Y+ +++  + K LE IWR S
Sbjct: 156 EFNVTPRIGWQIDPFGHSAVQAYLLGAEVGFDSVFFGRIDYQDREKRYKEKTLEVIWRGS 215

Query: 328 WDAEETTDIFVHMMPFYSYDIPHTCGPEPAICCQFDFARSRGSSYEL---CPWRQDPVEI 387
                ++ IF    P  +Y+      P P            G  YE+    P  QD  ++
Sbjct: 216 KSLGSSSQIFAGAFP-TNYE------PPPG-----------GFYYEITDDSPVVQDDPDL 275

Query: 388 NKENVQERAMMLLDQYRKKSVLYRTNTLLIPLGDDFRYVSIDEAEAQFKNYQLLFDYINS 447
              NVQER    +     ++ + R N ++  +G DFRY     A   ++    L  Y+N 
Sbjct: 276 FDYNVQERVNAFVAAALDQANITRINHIMFTMGTDFRY---QYAHTWYRQMDKLIHYVNL 335

Query: 448 NPSLNAEAKFGTLEDYFRTLRDEAEKINYSLPGEVGSSQISGFPSLSGDFFTYADRQQDY 507
           +  +NA   F +    +   +  A +                +P  + D+F YADR   Y
Sbjct: 336 DGRVNA---FYSTPSIYTDAKHAANE---------------AWPLKTEDYFPYADRINAY 395

Query: 508 WSGYYVSRPFFKAVDRVLEQTLRAAEMILALLLGSCQRSQCEKLPVGFSYKLTAARRNLA 567
           W+GY+ SRP  K   RV+     AA   L    G  Q+               +    LA
Sbjct: 396 WTGYFTSRPALKRYVRVMSAYYLAARQ-LEFFKGRSQKGP----------NTDSLADALA 455

Query: 568 LFQHHDGVTGTAKDHVVRDYGVRMHTSLQDLHIFMSKGIEVLLGIRHEKSDQNPSQFEPE 627
           + QHHD V+GT+K HV  DY  R+     +    ++  +  L  +       NP+  +  
Sbjct: 456 IAQHHDAVSGTSKQHVANDYAKRLAIGYVEAESVVATSLAHLTKV---DPTLNPTFQQCL 515

Query: 628 QVRSKYDAQCVHKAIDLQEGIYQSVVFFNPLAQTREEVAMIIVNRTDVAVLDSNWTCLQS 687
            +   Y   C    ++L +G    V+ +NPL   R ++  + V   DV+V DS    ++S
Sbjct: 516 LLNISY---CPSSEVNLSDGKSLIVLAYNPLGWKRVDIVRLPVVGGDVSVHDSEGHEVES 575

Query: 688 QISP-------------EFQHDKANVFTGRHRIHWKISVPALGLQTYYIANGLFDCEKPK 747
           Q+ P             E    ++     ++ + + ++VP LG  TY I+          
Sbjct: 576 QLVPFTDEYVALRKYHVEAYLGQSPTQVPKYWLVFSVTVPPLGFTTYTISTA-------- 635

Query: 748 PAKLKFFSTSTSLPCATPYICSKADGDVAV--IGNQHQSLVFHGKQGLLHKIIN-KNGPQ 807
             K   +S+ +       Y+ +   G+ ++  IG+ H  L F   QG     +N +    
Sbjct: 636 -KKTDGYSSKS-------YVSNILKGEQSIINIGHGHLKLSFSTDQGTAINYVNGRTSMT 695

Query: 808 NVVNEEIALYTSW------------GSGAYLFKPNGDAESIIKAGGLTVITEGPLMQEVY 867
             V +  + Y+++             SGAY+F+PNG      +      +  GPL+ EV+
Sbjct: 696 EPVKQTFSYYSAYNGSNDKEPLIPQNSGAYVFRPNGTFPINPEGQVPLTVIHGPLVDEVH 755

Query: 868 SYPKTGWEKSPISHSTRLYSGGNTIQEHLIEMEYHVELLGKEFDD---KELIVRYKTDID 927
                 W    IS  TR+Y G    +EH +E+E+ V  +    DD   KE++ +  + + 
Sbjct: 756 QQINP-W----ISQITRVYKG----KEH-VEVEFIVGNI--PIDDGIGKEVVTQISSSLK 815

Query: 928 NKRIFYSDLNGFQ-ISRRESYD-------KIPLQGNYYPMPSLAFMEGSNGQRFSVHSRQ 971
           + + FY+D +G   I R   Y          P+ GNYYP+    +++ S  + FSV   +
Sbjct: 816 SNKTFYTDSSGRDYIKRIRDYRSDWKLDVNQPIAGNYYPINHGIYLQDSK-KEFSVMVDR 824