CmaCh14G005830 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh14G005830
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionDihydroorotase
LocationCma_Chr14: 2895780 .. 2898497 (-)
RNA-Seq ExpressionCmaCh14G005830
SyntenyCmaCh14G005830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAAAGACTTTGGCCCTTCCGTTCCAAGTACATATGCGTTTATTAGTAATACAATCCAATCTATTTATGTTCAATTGGTACTCGGTACTTCATTTTGTTACCATCATTTGTTCTTCAATCAACATTAATGATATCTTGTTTGAGCATTATAGGATATAATTTGAACATCATCGTGTGGTGTTAAATTATTATAGCTCCCCTAATCCGTTGAGAACTATATCAGTAATAATGAACTAAACTTGATTGATTTCAAAGCTTTGCTTTCCATTCACTGATTGATACATTCTTTATGGTAGCCACTGAAATCACCTAGGCAAAAGTTTGAAGGGCTCAAGTTCATAAGGCGTCAGGGGCCAAAAATGCAGCTCACACTTACTCAACCTGATGATTGGCATCTTCATCTTCGTGATGGCGATCTTTTAAAAGCTGTTCTCCCTCACAGGTAAATTTATCATGTCTATTCTAAATGATTCTATTTTAGCATTCATTCATGGCTTTGATACTGTGAGATCCACATCGGTTGGGGAGGAGAACGAAACACCCTTTATAAGTTAAAACCACGAGGGGAATCCCGAAAGGGAAAGCCCAAAGAGAACAATATCTGCCAGTGGCGGGTTTGGGCCGTTTCAGGTACCATATTAAACAAATGAGTTTCTTCTAAAGCTAATTGATAATGAGAGGATTAACTGTTGTATCTTATATAAGATTATGAGTTTTCTTCATCTTTCCTATGTGGGATCTTTTAACATTTTCTGTTCATTATTGTACTGAACTATTAATGTGCAGTGCAAGTAACTTTGGAAGGGCTATTGTGATGCCAAATTTGAAGCCACCCATCACAACCACAGCTGCTGCCGTGGCTTATCGAGATTCTATCTTGGAAGCATTACCGTCCAATAGCAACTTCACTCCTCTTATGACCCTTTATTTGACAGATACAATGAGTCCCAACGAGATCAAGCTTGCCAGTAAGTCTTAGCTTCCAACATTAATTCTTGGAAGACATTTTCCATTGAACTTCATATTTAGTGATTCTCTGTGGTTATATTCTATTTTTTAAACATGATGATGCATTGGCAGGAAGAAGTGGGGCTGTTTATGCTGTAAAATTGTATCCTGCTGGTGCTACAACCAATTCCCAAGACGGTGTAACAGATCTTTTCGGAAAGTGTCTGCCTGTCCTCGAGGAGATGGTTGAACAGGATATGCCTCTGTTGGTACTTTTGCTGATAGAACAACACTTTATTTCTCCAATATATAGGGAACAGTCGTTGGGGTTTCCTTTTAATTTAGAAAGAAAAATCTTGTAGGTTCATGGCGAGGTTACAAATCCTGATGTCGACGTGTTTGATCGTGAGAAGGTCTTCATTGAAACTGTGTTGAAACCTCTGATTGAGAGGCTTCCAAAACTGAAGGTGGTGATGGAGCATATCACTACTGCAGATGCTGCTAAGTTTGTTCAGTCTTGCGAAAATGGTATGCATCATTATTTTCATTAGTTATCGCTCGTTTATTCCGTATCACTTTTCATTATAACATATGATTACTAGTTTATGAGTTTTAAAATACTTCACAGGACTTTTAGCAGCAACGGTCACTCCACAACATCTTCTTCTCAACAGGAATTCTCTGTTTCAAGGAGGATTGCAGCCACATAATTACTGTCTTCCAGTACTCAAGAGAGAGACTCACAGTATGTTCCAACTAAATTTTGTGCTGATTCTTTATATTTTGGTCTTCTTGTTTGTCTGTGTCAATAGCCTTCGTGAAAAATAATCTGTAATGGCTTAGGCTCACCACTAGCAGATATTGTCCTCTTTGGGCTTTCTCTTTTGGGCTTTCCCTTAAGGTTTTTAAAACGCGTCTGCTAGGGAAAGGTTTCCACACCCTTATAAAGGGTGTTTCGTTCTCCTTCCCAACCGATGCAGAATATCACATAATCTCCTAACCATTATCAGATATTCCTATTGAAGTGAGAAGATTTTTGGAGGTTAGAATGGATTAATCTCAGCAGACGCTGGCTTTTGACTTTTTGTAATTGATCTTAGAATCACCATAGTCTAGTGATCAATAAGGCTTTGGGCGAATGGGTTCAAGTGAGTTTCCTTGGTAATCTAACGTAGAAGGGGCTTGATAGTTATCTTGAGATGATGATATTCGAGATGTGTATTAGCTGACTCAGATACACACGAATATAAATTATTGGCGACTTTTCACTATGAAAATCGAGAATCTCCCCTAAATGAAAGCTCTCGTCCTCTCATATAGGGCAGGCAATCGTGTCAGCTGTGACCAGTGGGAGTAAAAAGTTTTTCCTTGGAACGGATAGCGCTCCACATGATCGACGAAGGAAAGAATGTGCTTGTGGTTGTGCTGGTATATACAGTGCCCCCATTGCCTTATCATTATACGCAAAGGTGTTTGAAGAGGTATACTCTTCATCCTATTATGCTAATAATTCGTTCATGTCTTCATTTTTATCAAATCATCACTCTTTTTGTTCCCTTTTTGTCTACCAAGGCGTGTGCTCTTGATAAATTAGAAGCTTTTACAAGTTTCAATGGACCAGACTTCTATGGGCTCCCCAGAAACGCCTCAAAAATTACACTGCAAAAAGCTCCATGGAAGGTTCCCGAGTCCTTTTCTTTTCCTTTTGGGGACATTGTTCCGATGTTTGCAGGTGAGACACTTGAGTGGCAGCCATGCATCAACTAA

mRNA sequence

ATGACAAAGACTTTGGCCCTTCCGTTCCAAGTACATATGCGTTTATTACCACTGAAATCACCTAGGCAAAAGTTTGAAGGGCTCAAGTTCATAAGGCGTCAGGGGCCAAAAATGCAGCTCACACTTACTCAACCTGATGATTGGCATCTTCATCTTCGTGATGGCGATCTTTTAAAAGCTGTTCTCCCTCACAGTGCAAGTAACTTTGGAAGGGCTATTGTGATGCCAAATTTGAAGCCACCCATCACAACCACAGCTGCTGCCGTGGCTTATCGAGATTCTATCTTGGAAGCATTACCGTCCAATAGCAACTTCACTCCTCTTATGACCCTTTATTTGACAGATACAATGAGTCCCAACGAGATCAAGCTTGCCAGAAGAAGTGGGGCTGTTTATGCTGTAAAATTGTATCCTGCTGGTGCTACAACCAATTCCCAAGACGGTGTAACAGATCTTTTCGGAAAGTGTCTGCCTGTCCTCGAGGAGATGGTTGAACAGGATATGCCTCTGTTGGTTCATGGCGAGGTTACAAATCCTGATGTCGACGTGTTTGATCGTGAGAAGGTCTTCATTGAAACTGTGTTGAAACCTCTGATTGAGAGGCTTCCAAAACTGAAGGTGGTGATGGAGCATATCACTACTGCAGATGCTGCTAAGTTTGTTCAGTCTTGCGAAAATGGACTTTTAGCAGCAACGGTCACTCCACAACATCTTCTTCTCAACAGGAATTCTCTGTTTCAAGGAGGATTGCAGCCACATAATTACTGTCTTCCAGTACTCAAGAGAGAGACTCACAGGCAGGCAATCGTGTCAGCTGTGACCAGTGGGAGTAAAAAGTTTTTCCTTGGAACGGATAGCGCTCCACATGATCGACGAAGGAAAGAATGTGCTTGTGGTTGTGCTGGTATATACAGTGCCCCCATTGCCTTATCATTATACGCAAAGGTGTTTGAAGAGGCGTGTGCTCTTGATAAATTAGAAGCTTTTACAAGTTTCAATGGACCAGACTTCTATGGGCTCCCCAGAAACGCCTCAAAAATTACACTGCAAAAAGCTCCATGGAAGGTTCCCGAGTCCTTTTCTTTTCCTTTTGGGGACATTGTTCCGATGTTTGCAGGTGAGACACTTGAGTGGCAGCCATGCATCAACTAA

Coding sequence (CDS)

ATGACAAAGACTTTGGCCCTTCCGTTCCAAGTACATATGCGTTTATTACCACTGAAATCACCTAGGCAAAAGTTTGAAGGGCTCAAGTTCATAAGGCGTCAGGGGCCAAAAATGCAGCTCACACTTACTCAACCTGATGATTGGCATCTTCATCTTCGTGATGGCGATCTTTTAAAAGCTGTTCTCCCTCACAGTGCAAGTAACTTTGGAAGGGCTATTGTGATGCCAAATTTGAAGCCACCCATCACAACCACAGCTGCTGCCGTGGCTTATCGAGATTCTATCTTGGAAGCATTACCGTCCAATAGCAACTTCACTCCTCTTATGACCCTTTATTTGACAGATACAATGAGTCCCAACGAGATCAAGCTTGCCAGAAGAAGTGGGGCTGTTTATGCTGTAAAATTGTATCCTGCTGGTGCTACAACCAATTCCCAAGACGGTGTAACAGATCTTTTCGGAAAGTGTCTGCCTGTCCTCGAGGAGATGGTTGAACAGGATATGCCTCTGTTGGTTCATGGCGAGGTTACAAATCCTGATGTCGACGTGTTTGATCGTGAGAAGGTCTTCATTGAAACTGTGTTGAAACCTCTGATTGAGAGGCTTCCAAAACTGAAGGTGGTGATGGAGCATATCACTACTGCAGATGCTGCTAAGTTTGTTCAGTCTTGCGAAAATGGACTTTTAGCAGCAACGGTCACTCCACAACATCTTCTTCTCAACAGGAATTCTCTGTTTCAAGGAGGATTGCAGCCACATAATTACTGTCTTCCAGTACTCAAGAGAGAGACTCACAGGCAGGCAATCGTGTCAGCTGTGACCAGTGGGAGTAAAAAGTTTTTCCTTGGAACGGATAGCGCTCCACATGATCGACGAAGGAAAGAATGTGCTTGTGGTTGTGCTGGTATATACAGTGCCCCCATTGCCTTATCATTATACGCAAAGGTGTTTGAAGAGGCGTGTGCTCTTGATAAATTAGAAGCTTTTACAAGTTTCAATGGACCAGACTTCTATGGGCTCCCCAGAAACGCCTCAAAAATTACACTGCAAAAAGCTCCATGGAAGGTTCCCGAGTCCTTTTCTTTTCCTTTTGGGGACATTGTTCCGATGTTTGCAGGTGAGACACTTGAGTGGCAGCCATGCATCAACTAA

Protein sequence

MTKTLALPFQVHMRLLPLKSPRQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPDVDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPESFSFPFGDIVPMFAGETLEWQPCIN
Homology
BLAST of CmaCh14G005830 vs. ExPASy Swiss-Prot
Match: O04904 (Dihydroorotase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PYR4 PE=1 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 3.5e-171
Identity = 292/381 (76.64%), Postives = 333/381 (87.40%), Query Frame = 0

Query: 1   MTKTLALPFQVHMRLLPLKSPRQKFE-GLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLK 60
           M KTL  P+          S + KF+   + ++ +  +M+LT+TQPDDWHLHLRDGDLL 
Sbjct: 1   MIKTLVSPYS------GFGSQKLKFDRSSEKVKTRAVRMELTITQPDDWHLHLRDGDLLH 60

Query: 61  AVLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSP 120
           AV+PHSASNF RAIVMPNLKPP+T+TAAA+ YR  I++ALPS S+F PLMTLYLTD   P
Sbjct: 61  AVVPHSASNFKRAIVMPNLKPPVTSTAAAIIYRKFIMKALPSESSFDPLMTLYLTDKTLP 120

Query: 121 NEIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNP 180
            EI+LAR SG VYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMV+Q+MPLLVHGEVT+P
Sbjct: 121 EEIRLARESGVVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVKQNMPLLVHGEVTDP 180

Query: 181 DVDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLL 240
            +DVFDREK+FIETVL+PLI+RLP+LKVVMEHITT DA  FV+SC+ G + ATVTPQHLL
Sbjct: 181 SIDVFDREKIFIETVLQPLIQRLPQLKVVMEHITTMDAVNFVESCKEGSVGATVTPQHLL 240

Query: 241 LNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACG 300
           LNRN+LFQGGLQPHNYCLPVLKRE HR+AIV AVTSGSKKFFLGTDSAPH+R RKE +CG
Sbjct: 241 LNRNALFQGGLQPHNYCLPVLKREIHREAIVKAVTSGSKKFFLGTDSAPHERSRKESSCG 300

Query: 301 CAGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPES 360
           CAGIYSAPIALSLYAKVF+EA ALDKLEAFTSFNGPDFYGLPRN+SKITL+K+PWKVP+ 
Sbjct: 301 CAGIYSAPIALSLYAKVFDEAGALDKLEAFTSFNGPDFYGLPRNSSKITLKKSPWKVPDV 360

Query: 361 FSFPFGDIVPMFAGETLEWQP 381
           F+FPFG+IVPMFAGETL+WQP
Sbjct: 361 FNFPFGEIVPMFAGETLQWQP 375

BLAST of CmaCh14G005830 vs. ExPASy Swiss-Prot
Match: Q0JJD1 (Dihydroorotase, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=PYRC PE=2 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 2.4e-164
Identity = 271/345 (78.55%), Postives = 309/345 (89.57%), Query Frame = 0

Query: 36  PKMQLTLTQPDDWHLHLRDGDLLKAVLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSI 95
           P  +LT+T+PDDWHLHLR+GD+L AVLPHSA +FGRAIVMPNLKPP+TTTA A+ YR+ I
Sbjct: 56  PLQELTITRPDDWHLHLREGDVLAAVLPHSAMHFGRAIVMPNLKPPVTTTARALEYREEI 115

Query: 96  LEALPSNSNFTPLMTLYLTDTMSPNEIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGK 155
           L ALP  SNF PLMTLYLTD  SP EIKLA++SG V+AVKLYP+GATTNSQDGVTD+FGK
Sbjct: 116 LRALPPGSNFVPLMTLYLTDNTSPEEIKLAKKSGVVFAVKLYPSGATTNSQDGVTDIFGK 175

Query: 156 CLPVLEEMVEQDMPLLVHGEVTNPDVDVFDREKVFIETVLKPLIERLPKLKVVMEHITTA 215
           CLPVLEEM  Q+MPLLVHGEVT+  VD FDREKVFIE +L PL++RLP+LK+VMEHITT 
Sbjct: 176 CLPVLEEMARQEMPLLVHGEVTDQHVDTFDREKVFIEKILAPLVQRLPQLKIVMEHITTM 235

Query: 216 DAAKFVQSCENGLLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTS 275
           DA  FV+SC+ G +AATVTPQHLLLNRN+LFQGGLQPHNYCLPVLKRETHRQAIVSAVTS
Sbjct: 236 DAVNFVESCKEGHVAATVTPQHLLLNRNALFQGGLQPHNYCLPVLKRETHRQAIVSAVTS 295

Query: 276 GSKKFFLGTDSAPHDRRRKECACGCAGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGP 335
           GSK++FLGTDSAPHD+RRKEC+CGCAGIYSAP+ALSLYAKVFE+A ALDKLEAFTSFNGP
Sbjct: 296 GSKQYFLGTDSAPHDKRRKECSCGCAGIYSAPVALSLYAKVFEQAGALDKLEAFTSFNGP 355

Query: 336 DFYGLPRNASKITLQKAPWKVPESFSFPFGDIVPMFAGETLEWQP 381
           DFYGLPRN SKI L+K+ WKVP+++S+  G+IVPMF G TLEW P
Sbjct: 356 DFYGLPRNTSKIVLRKSAWKVPDTYSYSSGEIVPMFTGNTLEWLP 400

BLAST of CmaCh14G005830 vs. ExPASy Swiss-Prot
Match: B1XI96 (Dihydroorotase OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) OX=32049 GN=pyrC PE=3 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 9.9e-126
Identity = 221/341 (64.81%), Postives = 261/341 (76.54%), Query Frame = 0

Query: 39  QLTLTQPDDWHLHLRDGDLLKAVLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEA 98
           ++TLT+PDDWHLHLRDG+ LKAVLP +   F RAIVMPNLKPP+ T A A AY D IL A
Sbjct: 3   KITLTRPDDWHLHLRDGEALKAVLPDTVRQFARAIVMPNLKPPVRTVAEAAAYHDRILAA 62

Query: 99  LPSNSNFTPLMTLYLTDTMSPNEIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLP 158
           +P    F PLMTLYLTD  SP EI+ A+ SG V AVK YPAGATTNS  GVTD+  KC  
Sbjct: 63  IPPGRTFEPLMTLYLTDNTSPAEIQAAKESGFVKAVKYYPAGATTNSDLGVTDI-RKCDA 122

Query: 159 VLEEMVEQDMPLLVHGEVTNPDVDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAA 218
           V E M   D+PLL+HGEVT+  VDVFDREKVFIET L+PL ER P+L++V+EHITT DA 
Sbjct: 123 VFEAMQTVDLPLLLHGEVTDHRVDVFDREKVFIETYLRPLKERFPQLRIVLEHITTKDAV 182

Query: 219 KFVQSCENGLLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSK 278
           +FV + +   +AAT+TPQHLL NRNS+FQGG++PH YCLP+LKRETHR+A++ A TSG+ 
Sbjct: 183 EFVLASDE-KVAATITPQHLLFNRNSIFQGGIRPHFYCLPILKRETHREALLEAATSGNP 242

Query: 279 KFFLGTDSAPHDRRRKECACGCAGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFY 338
           KFFLGTDSAPH R RKE  CGCAG YSA  AL LYA VFE A ALDKLE F SF GPDFY
Sbjct: 243 KFFLGTDSAPHGRDRKESDCGCAGCYSALHALELYATVFEAADALDKLEGFASFYGPDFY 302

Query: 339 GLPRNASKITLQKAPWKVPESFSFPFGDIVPMFAGETLEWQ 380
            LPRN  KITL K PW++P++  FP   +VP++AG+ L W+
Sbjct: 303 QLPRNTEKITLTKTPWQIPDALPFPESSLVPLWAGQELSWK 341

BLAST of CmaCh14G005830 vs. ExPASy Swiss-Prot
Match: C1D672 (Dihydroorotase OS=Laribacter hongkongensis (strain HLHK9) OX=557598 GN=pyrC PE=3 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 1.2e-123
Identity = 223/341 (65.40%), Postives = 257/341 (75.37%), Query Frame = 0

Query: 40  LTLTQPDDWHLHLRDGDLLKAVLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEAL 99
           LTLT+PDDWHLHLRDG+ L AV  +SA  F RAIVMPNLKPP+TT   A AYR+ IL AL
Sbjct: 4   LTLTRPDDWHLHLRDGEALAAVAAYSAREFARAIVMPNLKPPVTTVVQAAAYRERILAAL 63

Query: 100 -PSNSNFTPLMTLYLTDTMSPNEIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLP 159
            P    F PLMTLYLTD   P EI     SG V+A+KLYPAGATTNS  GVT L G CLP
Sbjct: 64  PPGGPAFEPLMTLYLTDNTPPAEIDRVAESGFVHALKLYPAGATTNSDAGVTSLSG-CLP 123

Query: 160 VLEEMVEQDMPLLVHGEVTNPDVDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAA 219
            L  M E  +PLLVHGEVT+P +DVFDRE VFI+TVL+PL+  LP L+VVMEHITT  AA
Sbjct: 124 TLTRMAELGVPLLVHGEVTDPQIDVFDREAVFIDTVLQPLLAELPTLRVVMEHITTRQAA 183

Query: 220 KFVQSCENGLLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSK 279
           +FV +  +  +AATVTPQH+L+NRN+LF GGL+PH+YCLPVLKRE HR AI  AVTSGS 
Sbjct: 184 EFVLAAPDN-IAATVTPQHMLMNRNALFTGGLRPHHYCLPVLKREEHRAAIARAVTSGST 243

Query: 280 KFFLGTDSAPHDRRRKECACGCAGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFY 339
           +FFLGTDSAPH R  KE ACGCAGI+SA  AL LYA+ FE+A ALD+LEAF SFNGPDFY
Sbjct: 244 RFFLGTDSAPHARHAKEAACGCAGIFSAHAALPLYAEAFEDAGALDRLEAFASFNGPDFY 303

Query: 340 GLPRNASKITLQKAPWKVPESFSFPFGDIVPMFAGETLEWQ 380
            LPRN   +TL++  W VP S  +    +VP+ AGETL WQ
Sbjct: 304 RLPRNTGTVTLKREEWTVPASLPYGNDTLVPLRAGETLRWQ 342

BLAST of CmaCh14G005830 vs. ExPASy Swiss-Prot
Match: B0JQJ1 (Dihydroorotase OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=pyrC PE=3 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 6.6e-122
Identity = 215/341 (63.05%), Postives = 255/341 (74.78%), Query Frame = 0

Query: 39  QLTLTQPDDWHLHLRDGDLLKAVLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEA 98
           +LT+T+PDDWHLHLRDG  +KAVLPH+   F RAI+MPNLKPP+ + A A +YR+ IL A
Sbjct: 3   RLTMTRPDDWHLHLRDGAAMKAVLPHTVRQFARAIIMPNLKPPVRSVADAASYRERILAA 62

Query: 99  LPSNSNFTPLMTLYLTDTMSPNEIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLP 158
           +P    F PLMTLYLTD  SP EI  A+ S  V AVK YPAGATTNS  GVTDL  +C  
Sbjct: 63  VPEGQQFEPLMTLYLTDNTSPEEIVAAKASQFVKAVKYYPAGATTNSDLGVTDL-RRCDG 122

Query: 159 VLEEMVEQDMPLLVHGEVTNPDVDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAA 218
           VL  M + DMPLL+HGEVT+ D+DVFDREKVFIE  L PLI R PKL+VV EHITTADA 
Sbjct: 123 VLAAMEQVDMPLLLHGEVTDGDIDVFDREKVFIEKHLIPLITRFPKLRVVFEHITTADAV 182

Query: 219 KFVQSCENGLLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSK 278
           KFV S  N  +AAT+TPQHLL +RN LF GG++PH YCLP+LKRE HR A++ A TSG+ 
Sbjct: 183 KFVLSANNN-VAATITPQHLLFSRNILFTGGIRPHFYCLPILKREDHRLALLQAATSGNP 242

Query: 279 KFFLGTDSAPHDRRRKECACGCAGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFY 338
           KFFLGTDSAPH R  KE +CGCAG YSA  AL LYA+ FE   A+D+LE F SF+GPDFY
Sbjct: 243 KFFLGTDSAPHSRYSKESSCGCAGCYSALHALELYAEAFESVDAIDQLEGFASFHGPDFY 302

Query: 339 GLPRNASKITLQKAPWKVPESFSFPFGDIVPMFAGETLEWQ 380
            LPRN  +ITL K+PW++P+   FP   +VP+ AGE + WQ
Sbjct: 303 QLPRNTEQITLTKSPWRIPDELPFPESGLVPLRAGEEITWQ 341

BLAST of CmaCh14G005830 vs. ExPASy TrEMBL
Match: A0A6J1J0C7 (Dihydroorotase OS=Cucurbita maxima OX=3661 GN=LOC111482314 PE=3 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 9.7e-217
Identity = 377/383 (98.43%), Postives = 377/383 (98.43%), Query Frame = 0

Query: 1   MTKTLALPFQVHMRLLPLKSPRQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 60
           MTKTLALPFQ      PLKSPRQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA
Sbjct: 1   MTKTLALPFQ------PLKSPRQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 60

Query: 61  VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPN 120
           VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPN
Sbjct: 61  VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPN 120

Query: 121 EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD 180
           EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD
Sbjct: 121 EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD 180

Query: 181 VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL 240
           VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL
Sbjct: 181 VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL 240

Query: 241 NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC 300
           NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC
Sbjct: 241 NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC 300

Query: 301 AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPESF 360
           AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPESF
Sbjct: 301 AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPESF 360

Query: 361 SFPFGDIVPMFAGETLEWQPCIN 384
           SFPFGDIVPMFAGETLEWQPCIN
Sbjct: 361 SFPFGDIVPMFAGETLEWQPCIN 377

BLAST of CmaCh14G005830 vs. ExPASy TrEMBL
Match: A0A6J1F3S4 (Dihydroorotase OS=Cucurbita moschata OX=3662 GN=LOC111442116 PE=3 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 1.2e-214
Identity = 373/383 (97.39%), Postives = 375/383 (97.91%), Query Frame = 0

Query: 1   MTKTLALPFQVHMRLLPLKSPRQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 60
           MTKTLALPFQ      PLKSP QKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA
Sbjct: 1   MTKTLALPFQ------PLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 60

Query: 61  VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPN 120
           VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTM+PN
Sbjct: 61  VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMNPN 120

Query: 121 EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD 180
           EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD
Sbjct: 121 EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD 180

Query: 181 VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL 240
           VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL
Sbjct: 181 VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL 240

Query: 241 NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC 300
           NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC
Sbjct: 241 NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC 300

Query: 301 AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPESF 360
           AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRN SKITLQKAPWKVPESF
Sbjct: 301 AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESF 360

Query: 361 SFPFGDIVPMFAGETLEWQPCIN 384
           SFPFGDIVPMFAGETLEWQPC+N
Sbjct: 361 SFPFGDIVPMFAGETLEWQPCMN 377

BLAST of CmaCh14G005830 vs. ExPASy TrEMBL
Match: A0A5D3DPM6 (Dihydroorotase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007860 PE=3 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 1.9e-204
Identity = 356/383 (92.95%), Postives = 367/383 (95.82%), Query Frame = 0

Query: 1   MTKTLALPFQVHMRLLPLKSPRQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 60
           MTKTL LPFQ      PLKSP QKFEG+KFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA
Sbjct: 88  MTKTLVLPFQ------PLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 147

Query: 61  VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPN 120
           VLPHSAS FGRAIVMPNLKPPITTTAAAVAYRDSIL+ALPSNSNF PLMTLYLTDTMSPN
Sbjct: 148 VLPHSASTFGRAIVMPNLKPPITTTAAAVAYRDSILKALPSNSNFIPLMTLYLTDTMSPN 207

Query: 121 EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD 180
           EIKLAR+SGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD
Sbjct: 208 EIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD 267

Query: 181 VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL 240
           VD+FDREKVFIETVLKPLIERLPKLKVVMEHITTA+AAKFV SCE+ LLAATVTPQHLLL
Sbjct: 268 VDMFDREKVFIETVLKPLIERLPKLKVVMEHITTANAAKFVLSCEHALLAATVTPQHLLL 327

Query: 241 NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC 300
           NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPH+RRRKE +CGC
Sbjct: 328 NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHERRRKESSCGC 387

Query: 301 AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPESF 360
           AGIYSAPIALSLYAKVFEEA ALDKLEAFTSFNGPDFYGLPRN SKITLQKAPW+VP+SF
Sbjct: 388 AGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSF 447

Query: 361 SFPFGDIVPMFAGETLEWQPCIN 384
           +FPFGDIVPMFAGETLEWQPC N
Sbjct: 448 TFPFGDIVPMFAGETLEWQPCFN 464

BLAST of CmaCh14G005830 vs. ExPASy TrEMBL
Match: A0A1S3B7W2 (Dihydroorotase OS=Cucumis melo OX=3656 GN=LOC103486989 PE=3 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 1.9e-204
Identity = 356/383 (92.95%), Postives = 367/383 (95.82%), Query Frame = 0

Query: 1   MTKTLALPFQVHMRLLPLKSPRQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 60
           MTKTL LPFQ      PLKSP QKFEG+KFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA
Sbjct: 1   MTKTLVLPFQ------PLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 60

Query: 61  VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPN 120
           VLPHSAS FGRAIVMPNLKPPITTTAAAVAYRDSIL+ALPSNSNF PLMTLYLTDTMSPN
Sbjct: 61  VLPHSASTFGRAIVMPNLKPPITTTAAAVAYRDSILKALPSNSNFIPLMTLYLTDTMSPN 120

Query: 121 EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD 180
           EIKLAR+SGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD
Sbjct: 121 EIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD 180

Query: 181 VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL 240
           VD+FDREKVFIETVLKPLIERLPKLKVVMEHITTA+AAKFV SCE+ LLAATVTPQHLLL
Sbjct: 181 VDMFDREKVFIETVLKPLIERLPKLKVVMEHITTANAAKFVLSCEHALLAATVTPQHLLL 240

Query: 241 NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC 300
           NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPH+RRRKE +CGC
Sbjct: 241 NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHERRRKESSCGC 300

Query: 301 AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPESF 360
           AGIYSAPIALSLYAKVFEEA ALDKLEAFTSFNGPDFYGLPRN SKITLQKAPW+VP+SF
Sbjct: 301 AGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSF 360

Query: 361 SFPFGDIVPMFAGETLEWQPCIN 384
           +FPFGDIVPMFAGETLEWQPC N
Sbjct: 361 TFPFGDIVPMFAGETLEWQPCFN 377

BLAST of CmaCh14G005830 vs. ExPASy TrEMBL
Match: A0A5A7UFB1 (Dihydroorotase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G001630 PE=3 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 4.7e-203
Identity = 356/384 (92.71%), Postives = 367/384 (95.57%), Query Frame = 0

Query: 1   MTKTLALPFQVHMRLLPLKSPRQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 60
           MTKTL LPFQ      PLKSP QKFEG+KFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA
Sbjct: 88  MTKTLVLPFQ------PLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 147

Query: 61  VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPN 120
           VLPHSAS FGRAIVMPNLKPPITTTAAAVAYRDSIL+ALPSNSNF PLMTLYLTDTMSPN
Sbjct: 148 VLPHSASTFGRAIVMPNLKPPITTTAAAVAYRDSILKALPSNSNFIPLMTLYLTDTMSPN 207

Query: 121 EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLL-VHGEVTNP 180
           EIKLAR+SGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLL VHGEVTNP
Sbjct: 208 EIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVVHGEVTNP 267

Query: 181 DVDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLL 240
           DVD+FDREKVFIETVLKPLIERLPKLKVVMEHITTA+AAKFV SCE+ LLAATVTPQHLL
Sbjct: 268 DVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTANAAKFVLSCEHALLAATVTPQHLL 327

Query: 241 LNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACG 300
           LNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPH+RRRKE +CG
Sbjct: 328 LNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHERRRKESSCG 387

Query: 301 CAGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPES 360
           CAGIYSAPIALSLYAKVFEEA ALDKLEAFTSFNGPDFYGLPRN SKITLQKAPW+VP+S
Sbjct: 388 CAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKS 447

Query: 361 FSFPFGDIVPMFAGETLEWQPCIN 384
           F+FPFGDIVPMFAGETLEWQPC N
Sbjct: 448 FTFPFGDIVPMFAGETLEWQPCFN 465

BLAST of CmaCh14G005830 vs. NCBI nr
Match: XP_022983807.1 (dihydroorotase, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 762.3 bits (1967), Expect = 2.0e-216
Identity = 377/383 (98.43%), Postives = 377/383 (98.43%), Query Frame = 0

Query: 1   MTKTLALPFQVHMRLLPLKSPRQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 60
           MTKTLALPFQ      PLKSPRQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA
Sbjct: 1   MTKTLALPFQ------PLKSPRQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 60

Query: 61  VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPN 120
           VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPN
Sbjct: 61  VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPN 120

Query: 121 EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD 180
           EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD
Sbjct: 121 EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD 180

Query: 181 VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL 240
           VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL
Sbjct: 181 VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL 240

Query: 241 NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC 300
           NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC
Sbjct: 241 NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC 300

Query: 301 AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPESF 360
           AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPESF
Sbjct: 301 AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPESF 360

Query: 361 SFPFGDIVPMFAGETLEWQPCIN 384
           SFPFGDIVPMFAGETLEWQPCIN
Sbjct: 361 SFPFGDIVPMFAGETLEWQPCIN 377

BLAST of CmaCh14G005830 vs. NCBI nr
Match: XP_023528165.1 (dihydroorotase, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 755.7 bits (1950), Expect = 1.9e-214
Identity = 374/383 (97.65%), Postives = 374/383 (97.65%), Query Frame = 0

Query: 1   MTKTLALPFQVHMRLLPLKSPRQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 60
           MTKTLALPFQ      PLKSP QKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA
Sbjct: 1   MTKTLALPFQ------PLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 60

Query: 61  VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPN 120
           VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPN
Sbjct: 61  VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPN 120

Query: 121 EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD 180
           EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVE DMPLLVHGEVTNPD
Sbjct: 121 EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEHDMPLLVHGEVTNPD 180

Query: 181 VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL 240
           VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL
Sbjct: 181 VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL 240

Query: 241 NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC 300
           NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC
Sbjct: 241 NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC 300

Query: 301 AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPESF 360
           AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRN SKITLQKAPWKVPESF
Sbjct: 301 AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESF 360

Query: 361 SFPFGDIVPMFAGETLEWQPCIN 384
           SFPFGDIVPMFAGETLEWQPCIN
Sbjct: 361 SFPFGDIVPMFAGETLEWQPCIN 377

BLAST of CmaCh14G005830 vs. NCBI nr
Match: XP_022935161.1 (dihydroorotase, mitochondrial [Cucurbita moschata])

HSP 1 Score: 755.4 bits (1949), Expect = 2.4e-214
Identity = 373/383 (97.39%), Postives = 375/383 (97.91%), Query Frame = 0

Query: 1   MTKTLALPFQVHMRLLPLKSPRQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 60
           MTKTLALPFQ      PLKSP QKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA
Sbjct: 1   MTKTLALPFQ------PLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 60

Query: 61  VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPN 120
           VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTM+PN
Sbjct: 61  VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMNPN 120

Query: 121 EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD 180
           EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD
Sbjct: 121 EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD 180

Query: 181 VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL 240
           VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL
Sbjct: 181 VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL 240

Query: 241 NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC 300
           NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC
Sbjct: 241 NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC 300

Query: 301 AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPESF 360
           AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRN SKITLQKAPWKVPESF
Sbjct: 301 AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESF 360

Query: 361 SFPFGDIVPMFAGETLEWQPCIN 384
           SFPFGDIVPMFAGETLEWQPC+N
Sbjct: 361 SFPFGDIVPMFAGETLEWQPCMN 377

BLAST of CmaCh14G005830 vs. NCBI nr
Match: XP_038897131.1 (dihydroorotase, mitochondrial-like isoform X1 [Benincasa hispida])

HSP 1 Score: 734.2 bits (1894), Expect = 5.8e-208
Identity = 362/383 (94.52%), Postives = 369/383 (96.34%), Query Frame = 0

Query: 1   MTKTLALPFQVHMRLLPLKSPRQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 60
           MTKTL LPFQ      PLKSP QKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA
Sbjct: 1   MTKTLGLPFQ------PLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 60

Query: 61  VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPN 120
           VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSIL+ALPSNSNF PLMTLYLTDTMSPN
Sbjct: 61  VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPN 120

Query: 121 EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD 180
           EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD
Sbjct: 121 EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD 180

Query: 181 VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL 240
           VD+FDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCE+GLLAATVTPQHLLL
Sbjct: 181 VDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCEHGLLAATVTPQHLLL 240

Query: 241 NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC 300
           NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPH+RRRKEC+CGC
Sbjct: 241 NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHERRRKECSCGC 300

Query: 301 AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPESF 360
           AGIYSAPIALSLYAKVFEE  ALDKLEAFTSFNGPDFYGLPRN SKITL KAPW+VP+SF
Sbjct: 301 AGIYSAPIALSLYAKVFEEVGALDKLEAFTSFNGPDFYGLPRNTSKITLHKAPWQVPKSF 360

Query: 361 SFPFGDIVPMFAGETLEWQPCIN 384
           SFPFGDIVPMFAGETLEWQPC N
Sbjct: 361 SFPFGDIVPMFAGETLEWQPCFN 377

BLAST of CmaCh14G005830 vs. NCBI nr
Match: TYK25626.1 (dihydroorotase [Cucumis melo var. makuwa])

HSP 1 Score: 721.5 bits (1861), Expect = 3.9e-204
Identity = 356/383 (92.95%), Postives = 367/383 (95.82%), Query Frame = 0

Query: 1   MTKTLALPFQVHMRLLPLKSPRQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 60
           MTKTL LPFQ      PLKSP QKFEG+KFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA
Sbjct: 88  MTKTLVLPFQ------PLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKA 147

Query: 61  VLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPN 120
           VLPHSAS FGRAIVMPNLKPPITTTAAAVAYRDSIL+ALPSNSNF PLMTLYLTDTMSPN
Sbjct: 148 VLPHSASTFGRAIVMPNLKPPITTTAAAVAYRDSILKALPSNSNFIPLMTLYLTDTMSPN 207

Query: 121 EIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD 180
           EIKLAR+SGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD
Sbjct: 208 EIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPD 267

Query: 181 VDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLL 240
           VD+FDREKVFIETVLKPLIERLPKLKVVMEHITTA+AAKFV SCE+ LLAATVTPQHLLL
Sbjct: 268 VDMFDREKVFIETVLKPLIERLPKLKVVMEHITTANAAKFVLSCEHALLAATVTPQHLLL 327

Query: 241 NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGC 300
           NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPH+RRRKE +CGC
Sbjct: 328 NRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHERRRKESSCGC 387

Query: 301 AGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPESF 360
           AGIYSAPIALSLYAKVFEEA ALDKLEAFTSFNGPDFYGLPRN SKITLQKAPW+VP+SF
Sbjct: 388 AGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSF 447

Query: 361 SFPFGDIVPMFAGETLEWQPCIN 384
           +FPFGDIVPMFAGETLEWQPC N
Sbjct: 448 TFPFGDIVPMFAGETLEWQPCFN 464

BLAST of CmaCh14G005830 vs. TAIR 10
Match: AT4G22930.1 (pyrimidin 4 )

HSP 1 Score: 602.4 bits (1552), Expect = 2.5e-172
Identity = 292/381 (76.64%), Postives = 333/381 (87.40%), Query Frame = 0

Query: 1   MTKTLALPFQVHMRLLPLKSPRQKFE-GLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLK 60
           M KTL  P+          S + KF+   + ++ +  +M+LT+TQPDDWHLHLRDGDLL 
Sbjct: 1   MIKTLVSPYS------GFGSQKLKFDRSSEKVKTRAVRMELTITQPDDWHLHLRDGDLLH 60

Query: 61  AVLPHSASNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSP 120
           AV+PHSASNF RAIVMPNLKPP+T+TAAA+ YR  I++ALPS S+F PLMTLYLTD   P
Sbjct: 61  AVVPHSASNFKRAIVMPNLKPPVTSTAAAIIYRKFIMKALPSESSFDPLMTLYLTDKTLP 120

Query: 121 NEIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNP 180
            EI+LAR SG VYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMV+Q+MPLLVHGEVT+P
Sbjct: 121 EEIRLARESGVVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVKQNMPLLVHGEVTDP 180

Query: 181 DVDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLL 240
            +DVFDREK+FIETVL+PLI+RLP+LKVVMEHITT DA  FV+SC+ G + ATVTPQHLL
Sbjct: 181 SIDVFDREKIFIETVLQPLIQRLPQLKVVMEHITTMDAVNFVESCKEGSVGATVTPQHLL 240

Query: 241 LNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACG 300
           LNRN+LFQGGLQPHNYCLPVLKRE HR+AIV AVTSGSKKFFLGTDSAPH+R RKE +CG
Sbjct: 241 LNRNALFQGGLQPHNYCLPVLKREIHREAIVKAVTSGSKKFFLGTDSAPHERSRKESSCG 300

Query: 301 CAGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPES 360
           CAGIYSAPIALSLYAKVF+EA ALDKLEAFTSFNGPDFYGLPRN+SKITL+K+PWKVP+ 
Sbjct: 301 CAGIYSAPIALSLYAKVFDEAGALDKLEAFTSFNGPDFYGLPRNSSKITLKKSPWKVPDV 360

Query: 361 FSFPFGDIVPMFAGETLEWQP 381
           F+FPFG+IVPMFAGETL+WQP
Sbjct: 361 FNFPFGEIVPMFAGETLQWQP 375

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O049043.5e-17176.64Dihydroorotase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PYR4 PE=1 SV=1[more]
Q0JJD12.4e-16478.55Dihydroorotase, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=PYRC P... [more]
B1XI969.9e-12664.81Dihydroorotase OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) OX=320... [more]
C1D6721.2e-12365.40Dihydroorotase OS=Laribacter hongkongensis (strain HLHK9) OX=557598 GN=pyrC PE=3... [more]
B0JQJ16.6e-12263.05Dihydroorotase OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=pyrC PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1J0C79.7e-21798.43Dihydroorotase OS=Cucurbita maxima OX=3661 GN=LOC111482314 PE=3 SV=1[more]
A0A6J1F3S41.2e-21497.39Dihydroorotase OS=Cucurbita moschata OX=3662 GN=LOC111442116 PE=3 SV=1[more]
A0A5D3DPM61.9e-20492.95Dihydroorotase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00786... [more]
A0A1S3B7W21.9e-20492.95Dihydroorotase OS=Cucumis melo OX=3656 GN=LOC103486989 PE=3 SV=1[more]
A0A5A7UFB14.7e-20392.71Dihydroorotase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G00163... [more]
Match NameE-valueIdentityDescription
XP_022983807.12.0e-21698.43dihydroorotase, mitochondrial isoform X1 [Cucurbita maxima][more]
XP_023528165.11.9e-21497.65dihydroorotase, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022935161.12.4e-21497.39dihydroorotase, mitochondrial [Cucurbita moschata][more]
XP_038897131.15.8e-20894.52dihydroorotase, mitochondrial-like isoform X1 [Benincasa hispida][more]
TYK25626.13.9e-20492.95dihydroorotase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT4G22930.12.5e-17276.64pyrimidin 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.140coord: 38..380
e-value: 2.5E-126
score: 423.4
IPR004721Dihydroorotase homodimeric typePIRSFPIRSF001237Dihydroorotasecoord: 36..382
e-value: 1.4E-145
score: 482.5
IPR004721Dihydroorotase homodimeric typeTIGRFAMTIGR00856TIGR00856coord: 40..379
e-value: 4.3E-145
score: 480.9
IPR004721Dihydroorotase homodimeric typePANTHERPTHR43137DIHYDROOROTASEcoord: 37..380
IPR004721Dihydroorotase homodimeric typeHAMAPMF_00219PyrC_classIIcoord: 38..381
score: 47.071033
IPR004721Dihydroorotase homodimeric typeCDDcd01294DHOasecoord: 40..378
e-value: 0.0
score: 572.69
IPR006680Amidohydrolase-relatedPFAMPF01979Amidohydro_1coord: 47..356
e-value: 9.6E-27
score: 94.2
IPR002195Dihydroorotase, conserved sitePROSITEPS00482DIHYDROOROTASE_1coord: 47..55
IPR002195Dihydroorotase, conserved sitePROSITEPS00483DIHYDROOROTASE_2coord: 283..294
IPR032466Metal-dependent hydrolaseSUPERFAMILY51556Metallo-dependent hydrolasescoord: 39..379

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh14G005830.1CmaCh14G005830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process
biological_process GO:0044205 'de novo' UMP biosynthetic process
biological_process GO:0019856 pyrimidine nucleobase biosynthetic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004151 dihydroorotase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0016812 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides