CmaCh14G005550 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh14G005550
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionCytokinin dehydrogenase
LocationCma_Chr14: 2763291 .. 2767025 (+)
RNA-Seq ExpressionCmaCh14G005550
SyntenyCmaCh14G005550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTAGTAAAGACGCCCACCCACCCAAAGACAAGACAGCAAGTAAATAACGTCTGAGCCGAAGTTGAAACTGCTCATTAGACAGACAAGCTCTTTAAATTCTCTTCTTCCGCCTCCAATTACCTTGAGCTCTTCTACACCACTTCTTTTTTCTTTGCCTCATAAAGGAAACCAAGGAGAAGCTCAATATTTAGAAACATGCGGTCACCATTTCCTAGCTTTCTCAACCACAACAAAATCTTACTCTTCAAAATTTGCATTATTCTTTTCATAAACTGTGCTACTTATAGAACCAACCTTTGTTTCACCAAATGTTTCGGGGAGCCTACTGGTGACCCTCCTATACAATCTTCTTCTAAACTGCTTTCATCCTTGGAAACAATAAGCCTAGATGGACACTTTAGCTTTAAAAACAATCAAGCAGAAGCTAAAGATTTTGGCAAAAGATATCACATGCTACCCTTAGCAGTTCTACACCCACATTCAGTTCGTGATATTTCAATTACATTAAAGCATATTTTTCAGATGGGTTCAGGAACAGAACTTACAGTTGCTGCAAGGGGTCACGGCCATTCTCTTCAAGGTCAGGCACAAGCCTTAAGAGGCGTAGTTATCAACATGGAGTCCCTTAAGGAACCAGCCATGCAAGTTCACAGTGGAAACTTACCGTGGGTGGATGTCCCCGGAGGAGAGCTGTGGATAAATATCTTACACGAAACGCTTAAACATGGCTTAACACCAAAATCTTGGACTGACTACCTTCATCTTTCTGTGGGTGGTACTCTATCAAATGCTGGTATTAGCGGGCAAGCTTTTAGGCATGGACCTCAAATCAGTAACGTCCACCAGCTAGAAATTGTCACAGGTACTCTTTCTTCGCTTATTCACAAGTTTTCTATAATGTATATTGCATATATTGTTTAGAATTCCATACTAAGTAATAACGGTAAAATGATCACATATTTGTTTTCTCGCTTTTCATTCCTTCAGTGATTGCACAACTAGTTCTTTTGAACTTATAGTTTCTTTTCTGTCACCAAAGTTGGGTATAAACTTTGCTGACCTCCCCAAGTATAACAGTTAAAATGATATTTTCCAGCCAAATCATTGCTCAGGTGGCTTACCCTCAAATAGAACATTGGAAGTTCAAATCTCTCACATAACCTCCTCAAGTTCTTGAACCTATTTAAAAATCAAAACTACAAAACAAAAGCACGTTAACGGGAAATTTGATTTCCAAATTATCGAAATTGGATACTGATGATAAACCTTCCACCTATACTTGATCATCAGGCAAATTGCGCAAACCCCACAATTTAATTAGCATGGACCCCAAAACTTGACTTAAAAATTTTAATGTCAAATTAGCATTATTCCAGTACCTTTCTCTCTTAAGTATGTCCCTCACACATATTCCGTTTAAAATATTATATTTATTATCCCTGTACCAACGTTCTCTATCGAGCAATTAGGGAGGGACCGCAATAAAGATAGATTTTGTGATTATACTGTATGATAGAGGGAACAAAAATATTTAATAGAGCAGAGTGGTTGGGAGAAATAATTATTTAGAAAGAGACTTTTCTTTCTCATCAGATAGAACCTTTAATGAGGCACCAAAATAACATGAAGTTCGAGGGATTAATTGTTAGAAGAACAGTATAATTATGGAGATGCCCTTTTCATTCCATTTTGGGATGTAATCCTCAGTCCATAAACGCCATAAAATGTAGCAGTGCAAAAAATTTTAATACTTCGGTTATTGGCTGTAGGGATCCTCTGTAATTCCTCTTAGGTGAGAAGAAAAAATTCACGACCCAACTTATTATAAACTGCTCACTCTATGTTCCACCTCCAACTTAGATGCAAATCTCACTGTTGAGAAAATTGAGGGCAACAATAACATAAACCAAATATGAAACATGAATATAGACCATTATAAAATTTGATGAAGAGACTGAAAAACTAAGTAATTTCAATCACAAACAGAATTCCTTGATATGGGAAAAATAAAATTTTTCGTTGGTCCTAATTTTACTTCTCCAATTTACCTTAAAATTTTTCAATAATAAATTTTATCTCATGACAATCAAATGATATTAAATACATTTAGACTCTTATTTATGTTGAATAAGTAAATATTGTGGTTTATAATATTGTTGAAGAAGCTTTGCAAGTACTGCTTACAATTCGATGCAAACATGGATAATCTTATCAAGCTGAAGCTTCTTAAGAATCGAAAACGATCACCCAAGTTAACATGAATTTTTGTAATTTCCTTTTACGATGGAAACTGTTGACCCCTAGACTCTTTCATTTTGGCCAAATGGAGAAAGCCAAGGCAGACAAACCGATGTAGTTTCAATTGAGTGCAGGAAAGGGCGAGGTTGTTACCTGTTCAGCGCAGCAGAATGCAGACCTGTTTTACAGTGTACTTGGAGGCCTTGGTCAGTTTGGCATCATCACAAGGGCAAGGATTTCTCTAGAATCAGCACCAAAAATGGTAATATACCCTGCATTCCATCAGAATAGGTACATAATATAACATTTTTAGTGATATCTTGTAATGCAAAACACAATCCGCCATCTTTTGACAGGTAAAGTGGATAAGAACCTTGTATTCAGACTTCGCCATATTCTCCAAGGACCAGGAACATCTGATATCCACCAAGTACTCCTTTGATTATATTGAAGGCTTTGTTCTAATAAATAGAACTGGCATTCTCAATAATTGGAGATCTTCATTTGATCCCAAAGATCCAGTTCAAGCCAGCCAATTCGTCTCAGATAGAACAACACTGTATTGTCTAGAAATGGCCATGTACTTCAACTCTGAAGAGACAGAAACTATGAACAAGGTGAGCATTGCCATAGACATTATCAGATATCAAGCTCATGACAAACAGCCAAAAAAAACAGTTAAAAAAGAGTGTGTACCAAATAATGTCATTTCCAAACTCTATGTGGTTGTGCAGAGAATTGAGAAGTTGCTATCACAATTGAGGTACATTCCAGCTACTCTCTTTCAGTCGGAAGTTCCTTACGTTGAGTTTCTGGATAGAGTGCACATATCTGAGAAAAAACTCAGGGAAAAAGGATTGTGGGAGGTTCCTCATCCTTGGATGAATCTAATGATCCCAAGAAGCAAAATACTTGATTTTGCTGCAGAGGTGTTTGACAAAATTCTATCAGATTCTACCAGTGGTCCCATACTAATCTACCCGGTTAACAAATCCAAGTACTTGTTTCAATAGATTTGTCCCAGAAACTCTACACTTATAATTTCCCCATCCTAATACAAATCAGTTTGGGTTGTACAGGTGGAACAATAAAACATCAGTGGTCACACCGGACGAAGAAATTTTCTACCTAGTGGGTTTCTTATTTTCTGCACTGCCAACTTCAACCGGAAAACAAAGCTTAGAGTACATTTTAGCCCAAAACCAAATAATTCTGGACTACTGTGTCAGGGATCTTCCTGGATGTACGCAATATCTGCCTCATTATAGCTCCCAAGACGAGTGGCAAGCTCATTTTGGCTCCAAATGGAGGGTACTGATGGAGAGGAAATCTGCCTATGACCCATTATCAATCCTTGCCCCGGGACAAAGAATCTTCCAGAAAGCCACTCCTGTACAACATCTTGTATCTAAAACAAACCACAAAACGAGCATCCTAGAAGAAGAGAATGCCAAAATGATATGAAAGTTCAAATAAATTTAAAATGTATTCTGTCG

mRNA sequence

GGTAGTAAAGACGCCCACCCACCCAAAGACAAGACAGCAAGTAAATAACGTCTGAGCCGAAGTTGAAACTGCTCATTAGACAGACAAGCTCTTTAAATTCTCTTCTTCCGCCTCCAATTACCTTGAGCTCTTCTACACCACTTCTTTTTTCTTTGCCTCATAAAGGAAACCAAGGAGAAGCTCAATATTTAGAAACATGCGGTCACCATTTCCTAGCTTTCTCAACCACAACAAAATCTTACTCTTCAAAATTTGCATTATTCTTTTCATAAACTGTGCTACTTATAGAACCAACCTTTGTTTCACCAAATGTTTCGGGGAGCCTACTGGTGACCCTCCTATACAATCTTCTTCTAAACTGCTTTCATCCTTGGAAACAATAAGCCTAGATGGACACTTTAGCTTTAAAAACAATCAAGCAGAAGCTAAAGATTTTGGCAAAAGATATCACATGCTACCCTTAGCAGTTCTACACCCACATTCAGTTCGTGATATTTCAATTACATTAAAGCATATTTTTCAGATGGGTTCAGGAACAGAACTTACAGTTGCTGCAAGGGGTCACGGCCATTCTCTTCAAGGTCAGGCACAAGCCTTAAGAGGCGTAGTTATCAACATGGAGTCCCTTAAGGAACCAGCCATGCAAGTTCACAGTGGAAACTTACCGTGGGTGGATGTCCCCGGAGGAGAGCTGTGGATAAATATCTTACACGAAACGCTTAAACATGGCTTAACACCAAAATCTTGGACTGACTACCTTCATCTTTCTGTGGGTGGTACTCTATCAAATGCTGGTATTAGCGGGCAAGCTTTTAGGCATGGACCTCAAATCAGTAACGTCCACCAGCTAGAAATTGTCACAGGAAAGGGCGAGGTTGTTACCTGTTCAGCGCAGCAGAATGCAGACCTGTTTTACAGTGTACTTGGAGGCCTTGGTCAGTTTGGCATCATCACAAGGGCAAGGATTTCTCTAGAATCAGCACCAAAAATGGTAAAGTGGATAAGAACCTTGTATTCAGACTTCGCCATATTCTCCAAGGACCAGGAACATCTGATATCCACCAAGTACTCCTTTGATTATATTGAAGGCTTTGTTCTAATAAATAGAACTGGCATTCTCAATAATTGGAGATCTTCATTTGATCCCAAAGATCCAGTTCAAGCCAGCCAATTCGTCTCAGATAGAACAACACTGTATTGTCTAGAAATGGCCATGTACTTCAACTCTGAAGAGACAGAAACTATGAACAAGAGAATTGAGAAGTTGCTATCACAATTGAGGTACATTCCAGCTACTCTCTTTCAGTCGGAAGTTCCTTACGTTGAGTTTCTGGATAGAGTGCACATATCTGAGAAAAAACTCAGGGAAAAAGGATTGTGGGAGGTTCCTCATCCTTGGATGAATCTAATGATCCCAAGAAGCAAAATACTTGATTTTGCTGCAGAGGTGTTTGACAAAATTCTATCAGATTCTACCAGTGGTCCCATACTAATCTACCCGGTTAACAAATCCAAGTGGAACAATAAAACATCAGTGGTCACACCGGACGAAGAAATTTTCTACCTAGTGGGTTTCTTATTTTCTGCACTGCCAACTTCAACCGGAAAACAAAGCTTAGAGTACATTTTAGCCCAAAACCAAATAATTCTGGACTACTGTGTCAGGGATCTTCCTGGATGTACGCAATATCTGCCTCATTATAGCTCCCAAGACGAGTGGCAAGCTCATTTTGGCTCCAAATGGAGGGTACTGATGGAGAGGAAATCTGCCTATGACCCATTATCAATCCTTGCCCCGGGACAAAGAATCTTCCAGAAAGCCACTCCTGTACAACATCTTGTATCTAAAACAAACCACAAAACGAGCATCCTAGAAGAAGAGAATGCCAAAATGATATGAAAGTTCAAATAAATTTAAAATGTATTCTGTCG

Coding sequence (CDS)

ATGCGGTCACCATTTCCTAGCTTTCTCAACCACAACAAAATCTTACTCTTCAAAATTTGCATTATTCTTTTCATAAACTGTGCTACTTATAGAACCAACCTTTGTTTCACCAAATGTTTCGGGGAGCCTACTGGTGACCCTCCTATACAATCTTCTTCTAAACTGCTTTCATCCTTGGAAACAATAAGCCTAGATGGACACTTTAGCTTTAAAAACAATCAAGCAGAAGCTAAAGATTTTGGCAAAAGATATCACATGCTACCCTTAGCAGTTCTACACCCACATTCAGTTCGTGATATTTCAATTACATTAAAGCATATTTTTCAGATGGGTTCAGGAACAGAACTTACAGTTGCTGCAAGGGGTCACGGCCATTCTCTTCAAGGTCAGGCACAAGCCTTAAGAGGCGTAGTTATCAACATGGAGTCCCTTAAGGAACCAGCCATGCAAGTTCACAGTGGAAACTTACCGTGGGTGGATGTCCCCGGAGGAGAGCTGTGGATAAATATCTTACACGAAACGCTTAAACATGGCTTAACACCAAAATCTTGGACTGACTACCTTCATCTTTCTGTGGGTGGTACTCTATCAAATGCTGGTATTAGCGGGCAAGCTTTTAGGCATGGACCTCAAATCAGTAACGTCCACCAGCTAGAAATTGTCACAGGAAAGGGCGAGGTTGTTACCTGTTCAGCGCAGCAGAATGCAGACCTGTTTTACAGTGTACTTGGAGGCCTTGGTCAGTTTGGCATCATCACAAGGGCAAGGATTTCTCTAGAATCAGCACCAAAAATGGTAAAGTGGATAAGAACCTTGTATTCAGACTTCGCCATATTCTCCAAGGACCAGGAACATCTGATATCCACCAAGTACTCCTTTGATTATATTGAAGGCTTTGTTCTAATAAATAGAACTGGCATTCTCAATAATTGGAGATCTTCATTTGATCCCAAAGATCCAGTTCAAGCCAGCCAATTCGTCTCAGATAGAACAACACTGTATTGTCTAGAAATGGCCATGTACTTCAACTCTGAAGAGACAGAAACTATGAACAAGAGAATTGAGAAGTTGCTATCACAATTGAGGTACATTCCAGCTACTCTCTTTCAGTCGGAAGTTCCTTACGTTGAGTTTCTGGATAGAGTGCACATATCTGAGAAAAAACTCAGGGAAAAAGGATTGTGGGAGGTTCCTCATCCTTGGATGAATCTAATGATCCCAAGAAGCAAAATACTTGATTTTGCTGCAGAGGTGTTTGACAAAATTCTATCAGATTCTACCAGTGGTCCCATACTAATCTACCCGGTTAACAAATCCAAGTGGAACAATAAAACATCAGTGGTCACACCGGACGAAGAAATTTTCTACCTAGTGGGTTTCTTATTTTCTGCACTGCCAACTTCAACCGGAAAACAAAGCTTAGAGTACATTTTAGCCCAAAACCAAATAATTCTGGACTACTGTGTCAGGGATCTTCCTGGATGTACGCAATATCTGCCTCATTATAGCTCCCAAGACGAGTGGCAAGCTCATTTTGGCTCCAAATGGAGGGTACTGATGGAGAGGAAATCTGCCTATGACCCATTATCAATCCTTGCCCCGGGACAAAGAATCTTCCAGAAAGCCACTCCTGTACAACATCTTGTATCTAAAACAAACCACAAAACGAGCATCCTAGAAGAAGAGAATGCCAAAATGATATGA

Protein sequence

MRSPFPSFLNHNKILLFKICIILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLETISLDGHFSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAARGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLTPKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFYSVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFVLINRTGILNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQLRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFDKILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQNQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQKATPVQHLVSKTNHKTSILEEENAKMI
Homology
BLAST of CmaCh14G005550 vs. ExPASy Swiss-Prot
Match: O22213 (Cytokinin dehydrogenase 1 OS=Arabidopsis thaliana OX=3702 GN=CKX1 PE=1 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 3.2e-205
Identity = 348/562 (61.92%), Postives = 427/562 (75.98%), Query Frame = 0

Query: 8   FLNHNKILLFKICIILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLETISLDGH 67
           F   N      I +IL ++C   RTNLC       P  + P  + S + SSL ++ L+G+
Sbjct: 9   FHRQNNKTFLGIFMILVLSCIPGRTNLCSNHSVSTPK-ELPSSNPSDIRSSLVSLDLEGY 68

Query: 68  FSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAARGHGHSL 127
            SF +    AKDFG RY + PLA+LHP SV DIS  +KHI  +GS + LTVAARGHGHSL
Sbjct: 69  ISFDDVHNVAKDFGNRYQLPPLAILHPRSVFDISSMMKHIVHLGSTSNLTVAARGHGHSL 128

Query: 128 QGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLTPKSWTDY 187
           QGQA A +GVVI MESL+ P ++++ G  P+VDV GGE+WINIL ETLK+GL+PKSWTDY
Sbjct: 129 QGQALAHQGVVIKMESLRSPDIRIYKGKQPYVDVSGGEIWINILRETLKYGLSPKSWTDY 188

Query: 188 LHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFYSVLGGLG 247
           LHL+VGGTLSNAGISGQAF+HGPQI+NV+QLEIVTGKGEVVTCS ++N++LF+SVLGGLG
Sbjct: 189 LHLTVGGTLSNAGISGQAFKHGPQINNVYQLEIVTGKGEVVTCSEKRNSELFFSVLGGLG 248

Query: 248 QFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFVLINRTGI 307
           QFGIITRARISLE AP MVKWIR LYSDF+ FS+DQE+LIS + +FDY+EGFV+INRT +
Sbjct: 249 QFGIITRARISLEPAPHMVKWIRVLYSDFSAFSRDQEYLISKEKTFDYVEGFVIINRTDL 308

Query: 308 LNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQLRYIPAT 367
           LNNWRSSF P D  QAS+F SD  TLYCLE+  YFN EE  +M++   KLLS+L YIP+T
Sbjct: 309 LNNWRSSFSPNDSTQASRFKSDGKTLYCLEVVKYFNPEEASSMDQETGKLLSELNYIPST 368

Query: 368 LFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFDKILSDST 427
           LF SEVPY+EFLDRVHI+E+KLR KGLWEVPHPW+NL+IP+S I  FA EVF+ IL+ + 
Sbjct: 369 LFSSEVPYIEFLDRVHIAERKLRAKGLWEVPHPWLNLLIPKSSIYQFATEVFNNILTSNN 428

Query: 428 SGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQNQIILDY 487
           +GPILIYPVN+SKW   TS++TP+E+IFYLV FL SA+P S+GK  LEY+L QNQ ++++
Sbjct: 429 NGPILIYPVNQSKWKKHTSLITPNEDIFYLVAFLPSAVPNSSGKNDLEYLLKQNQRVMNF 488

Query: 488 CVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQKAT---- 547
           C        QYLPHY +Q EW++HFG +W    +RK AYDPL+ILAPGQRIFQK T    
Sbjct: 489 CAAANLNVKQYLPHYETQKEWKSHFGKRWETFAQRKQAYDPLAILAPGQRIFQKTTGKLS 548

Query: 548 PVQHLVSKTN------HKTSIL 560
           P+Q   SK        H  SIL
Sbjct: 549 PIQLAKSKATGSPQRYHYASIL 569

BLAST of CmaCh14G005550 vs. ExPASy Swiss-Prot
Match: Q5JLP4 (Cytokinin dehydrogenase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX4 PE=2 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 2.8e-193
Identity = 319/498 (64.06%), Postives = 397/498 (79.72%), Query Frame = 0

Query: 49  IQSSSKLLSSLETISLDGHFSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIF 108
           +     +++SL  + LDGHFSF +  A A+DFG R  +LP AVLHP SV D++ T++ +F
Sbjct: 28  VPDEDDVVASLGALRLDGHFSFDDAHAAARDFGNRCSLLPAAVLHPGSVSDVAATVRRVF 87

Query: 109 QMGSGTELTVAARGHGHSLQGQAQALRGVVINMESLKEP---AMQVHSGNLPWVDVPGGE 168
           Q+G  + LTVAARGHGHSL GQ+QA  G+V+ MESL      A++VH G  P VD PGGE
Sbjct: 88  QLGRSSPLTVAARGHGHSLLGQSQAAGGIVVKMESLAAAAARAVRVHGGASPHVDAPGGE 147

Query: 169 LWINILHETLKHGLTPKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKG 228
           LWIN+LHETLKHGL P+SWTDYLHL+VGGTLSNAG+SGQAFRHGPQ+SNV+QLEIVTG+G
Sbjct: 148 LWINVLHETLKHGLAPRSWTDYLHLTVGGTLSNAGVSGQAFRHGPQVSNVNQLEIVTGRG 207

Query: 229 EVVTCSAQQNADLFYSVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEH 288
           EVVTCS + N+DLFY+ LGGLGQFGIITRARI+LE APKMV+WIR LYSDF  F++DQE 
Sbjct: 208 EVVTCSHEVNSDLFYAALGGLGQFGIITRARIALEPAPKMVRWIRVLYSDFETFTEDQEK 267

Query: 289 LISTKYSFDYIEGFVLINRTGILNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSE 348
           LI+++ +FDYIEGFV+INRTGILNNWR+SF P+DPVQASQF SD   LYCLE+ M FN +
Sbjct: 268 LIASEKTFDYIEGFVIINRTGILNNWRTSFKPQDPVQASQFQSDGRVLYCLELTMNFNHD 327

Query: 349 ETETMNKRIEKLLSQLRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLM 408
           E + M + +  LLS+LRYI +TLF ++V Y+EFLDRVH SE KLR +GLWEVPHPW+NL+
Sbjct: 328 EADIMEQEVGALLSRLRYISSTLFYTDVTYLEFLDRVHTSELKLRAQGLWEVPHPWLNLL 387

Query: 409 IPRSKILDFAAEVFDKILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSAL 468
           IPRS +  FA EVF KIL DS +GPIL+YPVN++KW+N+TSVV PDEEIFYLVGFL SA 
Sbjct: 388 IPRSTVHKFAKEVFGKILKDSNNGPILLYPVNRTKWDNRTSVVIPDEEIFYLVGFLSSA- 447

Query: 469 PTSTGKQSLEYILAQNQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSA 528
           P+S+G  S+E+ +  N  I+D+C ++  G  QYL  Y++Q +W+AHFG++W     RK  
Sbjct: 448 PSSSGHGSVEHAMNLNNKIVDFCEKNGVGMKQYLAPYTTQKQWKAHFGARWETFERRKHT 507

Query: 529 YDPLSILAPGQRIFQKAT 544
           YDPL+ILAPGQRIF KA+
Sbjct: 508 YDPLAILAPGQRIFPKAS 524

BLAST of CmaCh14G005550 vs. ExPASy Swiss-Prot
Match: Q75K78 (Cytokinin dehydrogenase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX9 PE=2 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 3.8e-190
Identity = 310/487 (63.66%), Postives = 392/487 (80.49%), Query Frame = 0

Query: 57  SSLETISLDGHFSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTEL 116
           SSLE ++LDGHFSF +  A A+DFG      P+AVLHP SV DI+ T++H+F MG  + L
Sbjct: 32  SSLEELTLDGHFSFHDVSAAAQDFGNLSSFPPVAVLHPGSVADIATTIRHVFLMGEHSTL 91

Query: 117 TVAARGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLK 176
           TVAARGHGHSL GQ+QA  G++I+MESL+   M+V+ G  P+VD  GGELWIN+LHETLK
Sbjct: 92  TVAARGHGHSLYGQSQAAEGIIISMESLQSNTMRVNPGVSPYVDASGGELWINVLHETLK 151

Query: 177 HGLTPKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNA 236
           +GL PKSWTDYLHL+VGGTLSNAG+SGQ FRHGPQISNV++LEIVTG+G+V+TCS +QN+
Sbjct: 152 YGLAPKSWTDYLHLTVGGTLSNAGVSGQTFRHGPQISNVNELEIVTGRGDVITCSPEQNS 211

Query: 237 DLFYSVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYI 296
           DLF++ LGGLGQFG+ITRARI LE APKMV+W+R LY DF  F++DQE LIS + +FDYI
Sbjct: 212 DLFHAALGGLGQFGVITRARIPLEPAPKMVRWLRVLYLDFTSFTEDQEMLISAEKTFDYI 271

Query: 297 EGFVLINRTGILNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEK 356
           EGFV+INRTGILNNWRSSF+P+DPV++SQF SD   L+CLEM   FN +E + M + +  
Sbjct: 272 EGFVIINRTGILNNWRSSFNPQDPVRSSQFESDGKVLFCLEMTKNFNPDEADVMEQEVNT 331

Query: 357 LLSQLRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAA 416
           LLSQLRY+P++LF ++V Y+EFLDRVH SE KLR KG+WEVPHPW+N++IPRS I  FA 
Sbjct: 332 LLSQLRYMPSSLFHTDVTYIEFLDRVHSSEMKLRAKGMWEVPHPWLNIIIPRSMIHKFAK 391

Query: 417 EVFDKILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEY 476
           EVF KIL DS +GPIL+YPVNKS+W+N+TSVV PDEE+FYLV FL SAL    G  ++++
Sbjct: 392 EVFGKILKDSNNGPILLYPVNKSRWDNRTSVVIPDEEVFYLVAFLSSAL----GPHNIKH 451

Query: 477 ILAQNQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQ 536
            L  N  I+++  +   G  QYLP+Y+++ EWQ+HFG++W    +RK AYDPL+ILAPGQ
Sbjct: 452 TLDLNYRIIEFSDKAGIGVKQYLPNYTTEQEWQSHFGARWDTFQQRKKAYDPLAILAPGQ 511

Query: 537 RIFQKAT 544
           RIFQKA+
Sbjct: 512 RIFQKAS 514

BLAST of CmaCh14G005550 vs. ExPASy Swiss-Prot
Match: Q9LY71 (Cytokinin dehydrogenase 6 OS=Arabidopsis thaliana OX=3702 GN=CKX6 PE=2 SV=2)

HSP 1 Score: 662.9 bits (1709), Expect = 3.2e-189
Identity = 319/539 (59.18%), Postives = 405/539 (75.14%), Query Frame = 0

Query: 7   SFLNHNKILLFKICIILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLETISLDG 66
           S L    +L+ +   IL ++C  ++   CF                S  +SSL+ + L G
Sbjct: 7   SLLRKRTMLIVRSFTILLLSCIAFKLACCF----------------SSSISSLKALPLVG 66

Query: 67  HFSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAARGHGHS 126
           H  F++    +KDFG RY ++PLAVLHP SV DI+ T++HI+ MG+ ++LTVAARG GHS
Sbjct: 67  HLEFEHVHHASKDFGNRYQLIPLAVLHPKSVSDIASTIRHIWMMGTHSQLTVAARGRGHS 126

Query: 127 LQGQAQALRGVVINMESLKEPAMQVHS--GNLPWVDVPGGELWINILHETLKHGLTPKSW 186
           LQGQAQ   G+VI+MESL    +QV+S     P+VDV GGELWINILHETLK+GL PKSW
Sbjct: 127 LQGQAQTRHGIVIHMESLHPQKLQVYSVDSPAPYVDVSGGELWINILHETLKYGLAPKSW 186

Query: 187 TDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFYSVLG 246
           TDYLHL+VGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGE++ C+ +QN+DLF  VLG
Sbjct: 187 TDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEILNCTKRQNSDLFNGVLG 246

Query: 247 GLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTK-YSFDYIEGFVLIN 306
           GLGQFGIITRARI+LE AP MVKWIR LY DFA F+KDQE LIS + + FDYIEGFV+IN
Sbjct: 247 GLGQFGIITRARIALEPAPTMVKWIRVLYLDFAAFAKDQEQLISAQGHKFDYIEGFVIIN 306

Query: 307 RTGILNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQLRY 366
           RTG+LN+WR SF  ++P++ASQF  D  TLYCLE+A Y   +  + +N+ +++ LS+L Y
Sbjct: 307 RTGLLNSWRLSFTAEEPLEASQFKFDGRTLYCLELAKYLKQDNKDVINQEVKETLSELSY 366

Query: 367 IPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFDKIL 426
           + +TLF +EV Y  FLDRVH+SE KLR KG WEVPHPW+NL++PRSKI +FA  VF  IL
Sbjct: 367 VTSTLFTTEVAYEAFLDRVHVSEVKLRSKGQWEVPHPWLNLLVPRSKINEFARGVFGNIL 426

Query: 427 SDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQNQI 486
           +D+++GP+++YPVNKSKW+N+TS VTP+EE+FYLV  L SA P S GK  +E IL +N+ 
Sbjct: 427 TDTSNGPVIVYPVNKSKWDNQTSAVTPEEEVFYLVAILTSASPGSAGKDGVEEILRRNRR 486

Query: 487 ILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQKA 543
           IL++      G  QYLPHY++++EW++HFG KW   + RKS YDPL+ILAPG RIFQKA
Sbjct: 487 ILEFSEEAGIGLKQYLPHYTTREEWRSHFGDKWGEFVRRKSRYDPLAILAPGHRIFQKA 529

BLAST of CmaCh14G005550 vs. ExPASy Swiss-Prot
Match: Q67YU0 (Cytokinin dehydrogenase 5 OS=Arabidopsis thaliana OX=3702 GN=CKX5 PE=2 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 3.0e-147
Identity = 262/494 (53.04%), Postives = 351/494 (71.05%), Query Frame = 0

Query: 56  LSSLETISLDGHFSFKNNQ--AEAKDFGK-RYHMLPLAVLHPHSVRDISITLKHIFQMGS 115
           L  +  I +DGHF+   +   + + DFG  +    PLAVLHP S  D++  ++  +  GS
Sbjct: 32  LLRIGAIDVDGHFTVHPSDLASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAY--GS 91

Query: 116 GTELTVAARGHGHSLQGQAQALR-GVVINMES--LKEPAMQVHSGNLPWVDVPGGELWIN 175
            T   V+ARGHGHS+ GQA A R GVV+ M       P   V    + +VDV GGELW++
Sbjct: 92  ATAFPVSARGHGHSINGQAAAGRNGVVVEMNHGVTGTPKPLVRPDEM-YVDVWGGELWVD 151

Query: 176 ILHETLKHGLTPKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVT 235
           +L +TL+HGL PKSWTDYL+L+VGGTLSNAGISGQAF HGPQISNV +L++VTGKGEV+ 
Sbjct: 152 VLKKTLEHGLAPKSWTDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTGKGEVMR 211

Query: 236 CSAQQNADLFYSVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLIST 295
           CS ++N  LF+ VLGGLGQFGIITRARISLE AP+ V+WIR LYS F +F++DQE+LIS 
Sbjct: 212 CSEEENTRLFHGVLGGLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISM 271

Query: 296 --KYSFDYIEGFVLINRTGILNNWRSS-FDPKDPVQASQFVSDRTTLYCLEMAMYFNSEE 355
             +  FDY+EGFV+++  G++NNWRSS F P++PV+ S   S+ + LYCLE+   ++  +
Sbjct: 272 HGQLKFDYVEGFVIVDE-GLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNYHDSD 331

Query: 356 TETMNKRIEKLLSQLRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMI 415
           +E +++ +E L+ +L +IP ++F +++ YV+FLDRVH +E KLR K LWEVPHPW+NL +
Sbjct: 332 SEIVDQEVEILMKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFV 391

Query: 416 PRSKILDFAAEVFDKILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALP 475
           P+S+I DF   VF  IL + TSGPILIYP+NK KW+ ++S VTPDEE+FYLV  L SAL 
Sbjct: 392 PKSRISDFDKGVFKGILGNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALT 451

Query: 476 TSTGKQSLEYILAQNQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAY 535
                Q LEY+  QN+ IL++C +      QYLPH+++Q+EW AHFG KW      K+ +
Sbjct: 452 DGEETQKLEYLKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEF 511

Query: 536 DPLSILAPGQRIFQ 541
           DP  ILA GQRIFQ
Sbjct: 512 DPRHILATGQRIFQ 521

BLAST of CmaCh14G005550 vs. ExPASy TrEMBL
Match: A0A6J1J8K4 (Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111482266 PE=3 SV=1)

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MRSPFPSFLNHNKILLFKICIILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE 60
           MRSPFPSFLNHNKILLFKICIILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE
Sbjct: 1   MRSPFPSFLNHNKILLFKICIILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE 60

Query: 61  TISLDGHFSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA 120
           TISLDGHFSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA
Sbjct: 61  TISLDGHFSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA 120

Query: 121 RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT 180
           RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT
Sbjct: 121 RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT 180

Query: 181 PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFY 240
           PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFY
Sbjct: 181 PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFY 240

Query: 241 SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFV 300
           SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFV
Sbjct: 241 SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFV 300

Query: 301 LINRTGILNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQ 360
           LINRTGILNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQ
Sbjct: 301 LINRTGILNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQ 360

Query: 361 LRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFD 420
           LRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFD
Sbjct: 361 LRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFD 420

Query: 421 KILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQ 480
           KILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQ
Sbjct: 421 KILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQ 480

Query: 481 NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQ 540
           NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQ
Sbjct: 481 NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQ 540

Query: 541 KATPVQHLVSKTNHKTSILEEENAKMI 568
           KATPVQHLVSKTNHKTSILEEENAKMI
Sbjct: 541 KATPVQHLVSKTNHKTSILEEENAKMI 567

BLAST of CmaCh14G005550 vs. ExPASy TrEMBL
Match: A0A6J1F2R1 (Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111441651 PE=3 SV=1)

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 551/567 (97.18%), Postives = 560/567 (98.77%), Query Frame = 0

Query: 1   MRSPFPSFLNHNKILLFKICIILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE 60
           MRSPFPSFLN+NKIL FKICIILF+NCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE
Sbjct: 1   MRSPFPSFLNNNKILHFKICIILFMNCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE 60

Query: 61  TISLDGHFSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA 120
           TISLDGHFSFKNNQA AKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA
Sbjct: 61  TISLDGHFSFKNNQAAAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA 120

Query: 121 RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT 180
           RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT
Sbjct: 121 RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT 180

Query: 181 PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFY 240
           PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLE+VTGKGEV+TCSAQQNADLFY
Sbjct: 181 PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGEVITCSAQQNADLFY 240

Query: 241 SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFV 300
           SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFA+FSKDQEHLISTKYSFDYIEGFV
Sbjct: 241 SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAVFSKDQEHLISTKYSFDYIEGFV 300

Query: 301 LINRTGILNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQ 360
           LINRTGILNNWRSSFDP DPVQASQFVSDR TLYCLEMAMYFNSEETETMNK++EKLLSQ
Sbjct: 301 LINRTGILNNWRSSFDPNDPVQASQFVSDRATLYCLEMAMYFNSEETETMNKKVEKLLSQ 360

Query: 361 LRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFD 420
           L YIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKI DFAAEVFD
Sbjct: 361 LSYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKIHDFAAEVFD 420

Query: 421 KILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQ 480
           KILSDST+GPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLE ILAQ
Sbjct: 421 KILSDSTNGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLENILAQ 480

Query: 481 NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQ 540
           NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFG+KWRVLMERKSAYDPLSILAPGQRIFQ
Sbjct: 481 NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGNKWRVLMERKSAYDPLSILAPGQRIFQ 540

Query: 541 KATPVQHLVSKTNHKTSILEEENAKMI 568
           KATPVQHLVSKTNHKTSILEEENAKMI
Sbjct: 541 KATPVQHLVSKTNHKTSILEEENAKMI 567

BLAST of CmaCh14G005550 vs. ExPASy TrEMBL
Match: A0A1S3B8H8 (Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103486946 PE=3 SV=1)

HSP 1 Score: 1047.3 bits (2707), Expect = 2.2e-302
Identity = 505/567 (89.07%), Postives = 532/567 (93.83%), Query Frame = 0

Query: 1   MRSPFPSFLNHNKILLFKICIILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE 60
           M SPF +FLNH   LLFKI +ILF+NCA YR NLCFTKC GEPT DP IQSSSK+LSSLE
Sbjct: 1   MGSPFSNFLNHKNFLLFKISMILFMNCAIYRPNLCFTKCLGEPTDDPVIQSSSKMLSSLE 60

Query: 61  TISLDGHFSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA 120
           TI+LDG+FSFKNNQ  AKDFG RY+MLPLAVLHPHS+RDISITLKHIF+MGSGTELTVAA
Sbjct: 61  TINLDGYFSFKNNQEAAKDFGNRYNMLPLAVLHPHSIRDISITLKHIFEMGSGTELTVAA 120

Query: 121 RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT 180
           RGHGHSLQGQAQAL+G+VINMESLKEPAMQVHSGNL WVDV GGELWINILHETLK+GLT
Sbjct: 121 RGHGHSLQGQAQALQGIVINMESLKEPAMQVHSGNLSWVDVHGGELWINILHETLKYGLT 180

Query: 181 PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFY 240
           PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLE+VTGKGEVVTCS Q+N+DLFY
Sbjct: 181 PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGEVVTCSEQENSDLFY 240

Query: 241 SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFV 300
           SVLGGLGQFGIITRARISLESAP+MVKWIRTLYSDFAIFSKDQEHLIS+KYSFDYIEGFV
Sbjct: 241 SVLGGLGQFGIITRARISLESAPEMVKWIRTLYSDFAIFSKDQEHLISSKYSFDYIEGFV 300

Query: 301 LINRTGILNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQ 360
           LINRTGILNNWRSSFDPKDPVQASQF++DRTTLYCLEMAMYFN E+ ET+NKR+EKLLSQ
Sbjct: 301 LINRTGILNNWRSSFDPKDPVQASQFITDRTTLYCLEMAMYFNFEDKETLNKRVEKLLSQ 360

Query: 361 LRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFD 420
           L YIPATLF SEVPY EFLDRVH SEKKLR KGLWEVPHPW+NLMIPRS I DFAAEVF 
Sbjct: 361 LSYIPATLFLSEVPYEEFLDRVHTSEKKLRAKGLWEVPHPWLNLMIPRSNIRDFAAEVFG 420

Query: 421 KILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQ 480
           KILSDSTSGPILIYPVNKSKWNNKTS+VTPDEEIFYLVGFLFSALPTSTGK+SLEYILAQ
Sbjct: 421 KILSDSTSGPILIYPVNKSKWNNKTSLVTPDEEIFYLVGFLFSALPTSTGKESLEYILAQ 480

Query: 481 NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQ 540
           NQIILDYC R LPGC QYLPHY SQDEWQAHFGSKWRVL ERKSAYDPLSILAPGQR+FQ
Sbjct: 481 NQIILDYCARALPGCKQYLPHYCSQDEWQAHFGSKWRVLKERKSAYDPLSILAPGQRVFQ 540

Query: 541 KATPVQHLVSKTNHKTSILEEENAKMI 568
           K TPVQHL+SKT HK SILEEENAKMI
Sbjct: 541 KVTPVQHLISKTKHKKSILEEENAKMI 567

BLAST of CmaCh14G005550 vs. ExPASy TrEMBL
Match: A0A5D3DPV6 (Cytokinin dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007350 PE=3 SV=1)

HSP 1 Score: 1022.7 bits (2643), Expect = 5.9e-295
Identity = 492/547 (89.95%), Postives = 518/547 (94.70%), Query Frame = 0

Query: 21  IILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLETISLDGHFSFKNNQAEAKDF 80
           +ILF+NCA YR NLCFTKC GEPT DP IQSSSK+LSSLETI+LDG+FSFKNNQ  AKDF
Sbjct: 1   MILFMNCAIYRPNLCFTKCLGEPTDDPVIQSSSKMLSSLETINLDGYFSFKNNQEAAKDF 60

Query: 81  GKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAARGHGHSLQGQAQALRGVVIN 140
           G RY+MLPLAVLHPHS+RDISITLKHIF+MGSGTELTVAARGHGHSLQGQAQAL+G+VIN
Sbjct: 61  GNRYNMLPLAVLHPHSIRDISITLKHIFEMGSGTELTVAARGHGHSLQGQAQALQGIVIN 120

Query: 141 MESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLTPKSWTDYLHLSVGGTLSNAG 200
           MESLKEPAMQVHSGNL WVDV GGELWINILHETLK+GLTPKSWTDYLHLSVGGTLSNAG
Sbjct: 121 MESLKEPAMQVHSGNLSWVDVHGGELWINILHETLKYGLTPKSWTDYLHLSVGGTLSNAG 180

Query: 201 ISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFYSVLGGLGQFGIITRARISLE 260
           ISGQAFRHGPQISNVHQLE+VTGKGEVVTCS Q+N+DLFYSVLGGLGQFGIITRARISLE
Sbjct: 181 ISGQAFRHGPQISNVHQLEVVTGKGEVVTCSEQENSDLFYSVLGGLGQFGIITRARISLE 240

Query: 261 SAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFVLINRTGILNNWRSSFDPKDP 320
           SAP+MVKWIRTLYSDFAIFSKDQEHLIS+KYSFDYIEGFVLINRTGILNNWRSSFDPKDP
Sbjct: 241 SAPEMVKWIRTLYSDFAIFSKDQEHLISSKYSFDYIEGFVLINRTGILNNWRSSFDPKDP 300

Query: 321 VQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQLRYIPATLFQSEVPYVEFLD 380
           VQASQF++DRTTLYCLEMAMYFN E+ ET+NKR+EKLLSQL YIPATLF SEVPY EFLD
Sbjct: 301 VQASQFITDRTTLYCLEMAMYFNFEDKETLNKRVEKLLSQLSYIPATLFLSEVPYEEFLD 360

Query: 381 RVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFDKILSDSTSGPILIYPVNKSK 440
           RVH SEKKLR KGLWEVPHPW+NLMIPRS I DFAAEVF KILSDSTSGPILIYPVNKSK
Sbjct: 361 RVHTSEKKLRAKGLWEVPHPWLNLMIPRSNIRDFAAEVFGKILSDSTSGPILIYPVNKSK 420

Query: 441 WNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQNQIILDYCVRDLPGCTQYLP 500
           WNNKTS+VTPDEEIFYLVGFLFSALPTSTGK+SLEYILAQNQIILDYC R LPGC QYLP
Sbjct: 421 WNNKTSLVTPDEEIFYLVGFLFSALPTSTGKESLEYILAQNQIILDYCARALPGCKQYLP 480

Query: 501 HYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQKATPVQHLVSKTNHKTSILE 560
           HY SQDEWQAHFGSKWRVL ERKSAYDPLSILAPGQR+FQK TPVQHL+SKT HK SILE
Sbjct: 481 HYCSQDEWQAHFGSKWRVLKERKSAYDPLSILAPGQRVFQKVTPVQHLISKTKHKKSILE 540

Query: 561 EENAKMI 568
           EENAKMI
Sbjct: 541 EENAKMI 547

BLAST of CmaCh14G005550 vs. ExPASy TrEMBL
Match: A0A2I4G6V9 (Cytokinin dehydrogenase OS=Juglans regia OX=51240 GN=LOC109005247 PE=3 SV=2)

HSP 1 Score: 803.1 bits (2073), Expect = 7.3e-229
Identity = 382/542 (70.48%), Postives = 452/542 (83.39%), Query Frame = 0

Query: 1   MRSPFPSFLNHNKILLFKICIILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE 60
           M S   SFL  N I+  KI ++LF++C   RTNLC    F   T  P   S++  LSSLE
Sbjct: 119 MGSASLSFLKQNNIIHLKILMVLFLSCIPDRTNLCSNNSFATLTVPPRFNSTNISLSSLE 178

Query: 61  TISLDGHFSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA 120
           T++LDG+FSF+NN   AKDFG  YH LP AVLHP SV DIS T++HIF+MGS +ELTVAA
Sbjct: 179 TLTLDGYFSFENNHHAAKDFGNLYHFLPSAVLHPKSVSDISTTIRHIFKMGSASELTVAA 238

Query: 121 RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT 180
           RGH HS+QGQAQA +G+VINMESL+   MQVH+G++P+VDV GGELWINILHETLKHGL 
Sbjct: 239 RGHAHSIQGQAQAHQGIVINMESLQGLKMQVHTGDMPYVDVSGGELWINILHETLKHGLA 298

Query: 181 PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFY 240
           PKSWTDYLHL+VGGTLSNAGISGQAF+HGPQI+NV+QLE+VTGKGEVVTCS +QNADLFY
Sbjct: 299 PKSWTDYLHLTVGGTLSNAGISGQAFQHGPQINNVYQLEVVTGKGEVVTCSEKQNADLFY 358

Query: 241 SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFV 300
           +VLGGLGQFGIITRARISL  APKMVKWI+ LYS+F+ FSKDQEHLIS++ SFDY+EGFV
Sbjct: 359 AVLGGLGQFGIITRARISLGPAPKMVKWIKVLYSEFSTFSKDQEHLISSENSFDYVEGFV 418

Query: 301 LINRTGILNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQ 360
            INRTGILNNWRSSF PKDP+QA++F SD  TLYCLEM  YFN +ET+TMN+ I+ LLSQ
Sbjct: 419 NINRTGILNNWRSSFSPKDPLQANKFTSDGRTLYCLEMVKYFNPDETKTMNQDIKNLLSQ 478

Query: 361 LRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFD 420
           L YIP+TLF SEV YVEFLDRVH+SE+KLR KGLWEVPHPW+NL++P+S+I  FA EVF 
Sbjct: 479 LNYIPSTLFLSEVSYVEFLDRVHVSERKLRAKGLWEVPHPWLNLLVPKSRISVFAEEVFG 538

Query: 421 KILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQ 480
            IL+D+++GPI+IYPVN+SKWNNKTS+VTPDE+IFYLV FL SA+P+STG   LE+IL Q
Sbjct: 539 NILTDTSNGPIIIYPVNQSKWNNKTSLVTPDEDIFYLVAFLSSAMPSSTGTDGLEHILTQ 598

Query: 481 NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQ 540
           N+ ILD+C     G  QYLPHY++ +EWQ HFGS W V ++RKS YDPL+ILAPGQRIFQ
Sbjct: 599 NKRILDFCATAQLGMKQYLPHYNTHEEWQTHFGSLWEVFVQRKSTYDPLAILAPGQRIFQ 658

Query: 541 KA 543
           KA
Sbjct: 659 KA 660

BLAST of CmaCh14G005550 vs. NCBI nr
Match: XP_022983738.1 (cytokinin dehydrogenase 4-like [Cucurbita maxima])

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MRSPFPSFLNHNKILLFKICIILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE 60
           MRSPFPSFLNHNKILLFKICIILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE
Sbjct: 1   MRSPFPSFLNHNKILLFKICIILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE 60

Query: 61  TISLDGHFSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA 120
           TISLDGHFSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA
Sbjct: 61  TISLDGHFSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA 120

Query: 121 RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT 180
           RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT
Sbjct: 121 RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT 180

Query: 181 PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFY 240
           PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFY
Sbjct: 181 PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFY 240

Query: 241 SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFV 300
           SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFV
Sbjct: 241 SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFV 300

Query: 301 LINRTGILNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQ 360
           LINRTGILNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQ
Sbjct: 301 LINRTGILNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQ 360

Query: 361 LRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFD 420
           LRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFD
Sbjct: 361 LRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFD 420

Query: 421 KILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQ 480
           KILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQ
Sbjct: 421 KILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQ 480

Query: 481 NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQ 540
           NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQ
Sbjct: 481 NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQ 540

Query: 541 KATPVQHLVSKTNHKTSILEEENAKMI 568
           KATPVQHLVSKTNHKTSILEEENAKMI
Sbjct: 541 KATPVQHLVSKTNHKTSILEEENAKMI 567

BLAST of CmaCh14G005550 vs. NCBI nr
Match: XP_023526449.1 (cytokinin dehydrogenase 9-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 558/567 (98.41%), Postives = 564/567 (99.47%), Query Frame = 0

Query: 1   MRSPFPSFLNHNKILLFKICIILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE 60
           MRSPFPSFLN+NKILLFKICIILF+NCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE
Sbjct: 1   MRSPFPSFLNNNKILLFKICIILFMNCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE 60

Query: 61  TISLDGHFSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA 120
           TISLDGHFSFKNNQA AKDFGKRYHMLPLAVLHPHS+RDISITLKHIFQMGSGTELTVAA
Sbjct: 61  TISLDGHFSFKNNQAAAKDFGKRYHMLPLAVLHPHSLRDISITLKHIFQMGSGTELTVAA 120

Query: 121 RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT 180
           RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT
Sbjct: 121 RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT 180

Query: 181 PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFY 240
           PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLE+VTGKGEV+TCSAQQNADLFY
Sbjct: 181 PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGEVITCSAQQNADLFY 240

Query: 241 SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFV 300
           SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFV
Sbjct: 241 SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFV 300

Query: 301 LINRTGILNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQ 360
           LINRTGILNNWRSSFDPKDPVQASQFVSDR TLYCLEMAMYFNSEETETMNKRIEKLLSQ
Sbjct: 301 LINRTGILNNWRSSFDPKDPVQASQFVSDRATLYCLEMAMYFNSEETETMNKRIEKLLSQ 360

Query: 361 LRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFD 420
           LRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKI DFAAEVFD
Sbjct: 361 LRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKIHDFAAEVFD 420

Query: 421 KILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQ 480
           KILSDST+GPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQ
Sbjct: 421 KILSDSTNGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQ 480

Query: 481 NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQ 540
           NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQ
Sbjct: 481 NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQ 540

Query: 541 KATPVQHLVSKTNHKTSILEEENAKMI 568
           KATPVQHLVSKTNHKTSILEEENAKMI
Sbjct: 541 KATPVQHLVSKTNHKTSILEEENAKMI 567

BLAST of CmaCh14G005550 vs. NCBI nr
Match: KAG7017671.1 (Cytokinin dehydrogenase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1141.3 bits (2951), Expect = 0.0e+00
Identity = 553/567 (97.53%), Postives = 562/567 (99.12%), Query Frame = 0

Query: 1   MRSPFPSFLNHNKILLFKICIILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE 60
           MRSPFPSFLN+NKILLFKICIILF+NCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE
Sbjct: 1   MRSPFPSFLNNNKILLFKICIILFMNCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE 60

Query: 61  TISLDGHFSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA 120
           TISLDGHFSFKNNQA AKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA
Sbjct: 61  TISLDGHFSFKNNQAAAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA 120

Query: 121 RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT 180
           RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT
Sbjct: 121 RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT 180

Query: 181 PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFY 240
           PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLE+VTGKGEV+TCSAQQNADLFY
Sbjct: 181 PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGEVITCSAQQNADLFY 240

Query: 241 SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFV 300
           SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFA+FSKDQEHLISTKYSFDYIEGFV
Sbjct: 241 SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAVFSKDQEHLISTKYSFDYIEGFV 300

Query: 301 LINRTGILNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQ 360
           LINRTGILNNWRSSFDP DPVQASQFVSDR TLYCLEMAMYFNSEETETMNK++EKLLSQ
Sbjct: 301 LINRTGILNNWRSSFDPNDPVQASQFVSDRATLYCLEMAMYFNSEETETMNKKVEKLLSQ 360

Query: 361 LRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFD 420
           L YIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKI DFAAEVFD
Sbjct: 361 LSYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKIHDFAAEVFD 420

Query: 421 KILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQ 480
           KILSDST+GPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQ
Sbjct: 421 KILSDSTNGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQ 480

Query: 481 NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQ 540
           NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFG+KWRVLMERKSAYDPLSILAPGQRIFQ
Sbjct: 481 NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGNKWRVLMERKSAYDPLSILAPGQRIFQ 540

Query: 541 KATPVQHLVSKTNHKTSILEEENAKMI 568
           KATPVQHLVSKTNHKTSILEEENAKMI
Sbjct: 541 KATPVQHLVSKTNHKTSILEEENAKMI 567

BLAST of CmaCh14G005550 vs. NCBI nr
Match: XP_022934489.1 (cytokinin dehydrogenase 4-like [Cucurbita moschata])

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 551/567 (97.18%), Postives = 560/567 (98.77%), Query Frame = 0

Query: 1   MRSPFPSFLNHNKILLFKICIILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE 60
           MRSPFPSFLN+NKIL FKICIILF+NCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE
Sbjct: 1   MRSPFPSFLNNNKILHFKICIILFMNCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE 60

Query: 61  TISLDGHFSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA 120
           TISLDGHFSFKNNQA AKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA
Sbjct: 61  TISLDGHFSFKNNQAAAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA 120

Query: 121 RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT 180
           RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT
Sbjct: 121 RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT 180

Query: 181 PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFY 240
           PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLE+VTGKGEV+TCSAQQNADLFY
Sbjct: 181 PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGEVITCSAQQNADLFY 240

Query: 241 SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFV 300
           SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFA+FSKDQEHLISTKYSFDYIEGFV
Sbjct: 241 SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAVFSKDQEHLISTKYSFDYIEGFV 300

Query: 301 LINRTGILNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQ 360
           LINRTGILNNWRSSFDP DPVQASQFVSDR TLYCLEMAMYFNSEETETMNK++EKLLSQ
Sbjct: 301 LINRTGILNNWRSSFDPNDPVQASQFVSDRATLYCLEMAMYFNSEETETMNKKVEKLLSQ 360

Query: 361 LRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFD 420
           L YIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKI DFAAEVFD
Sbjct: 361 LSYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKIHDFAAEVFD 420

Query: 421 KILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQ 480
           KILSDST+GPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLE ILAQ
Sbjct: 421 KILSDSTNGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLENILAQ 480

Query: 481 NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQ 540
           NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFG+KWRVLMERKSAYDPLSILAPGQRIFQ
Sbjct: 481 NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGNKWRVLMERKSAYDPLSILAPGQRIFQ 540

Query: 541 KATPVQHLVSKTNHKTSILEEENAKMI 568
           KATPVQHLVSKTNHKTSILEEENAKMI
Sbjct: 541 KATPVQHLVSKTNHKTSILEEENAKMI 567

BLAST of CmaCh14G005550 vs. NCBI nr
Match: XP_038905786.1 (cytokinin dehydrogenase 6-like [Benincasa hispida])

HSP 1 Score: 1060.1 bits (2740), Expect = 6.9e-306
Identity = 511/567 (90.12%), Postives = 537/567 (94.71%), Query Frame = 0

Query: 1   MRSPFPSFLNHNKILLFKICIILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLE 60
           M S F SFLNH KIL+FKI +ILF+NCATY+ NLCFTKCFGEPTGD PI+SSSK+LSSLE
Sbjct: 1   MGSLFSSFLNHKKILIFKISMILFMNCATYKPNLCFTKCFGEPTGDLPIKSSSKVLSSLE 60

Query: 61  TISLDGHFSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAA 120
           TISLDG+FSFKNNQ  AKDFG RYHMLPLAVLHP+SVRDISITLKHIFQMGSGTELTVAA
Sbjct: 61  TISLDGYFSFKNNQEAAKDFGNRYHMLPLAVLHPYSVRDISITLKHIFQMGSGTELTVAA 120

Query: 121 RGHGHSLQGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLT 180
           RGHGHSLQGQAQ L+GVVINMESLKEPAMQVH+GNLPWVDVPGGELWINILHETLK+GLT
Sbjct: 121 RGHGHSLQGQAQTLQGVVINMESLKEPAMQVHNGNLPWVDVPGGELWINILHETLKYGLT 180

Query: 181 PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFY 240
           PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLE+VTGKGE++TCS Q+NADLFY
Sbjct: 181 PKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGEIITCSEQENADLFY 240

Query: 241 SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFV 300
           SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLIS+KYSFDYIEGFV
Sbjct: 241 SVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISSKYSFDYIEGFV 300

Query: 301 LINRTGILNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQ 360
           LINRTGILNNWRSSFDPKDPVQASQF++D TTLYCLEMAMYFNSEE ETMNKR+EKLLSQ
Sbjct: 301 LINRTGILNNWRSSFDPKDPVQASQFIADNTTLYCLEMAMYFNSEEKETMNKRVEKLLSQ 360

Query: 361 LRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFD 420
           L YIPATLF SEVPYVEFLDRVHISEKKLR KGLWEVPHPW+NLMIPRSKI DFA EVF 
Sbjct: 361 LSYIPATLFLSEVPYVEFLDRVHISEKKLRTKGLWEVPHPWLNLMIPRSKIHDFATEVFG 420

Query: 421 KILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQ 480
           KILSDSTSGPILIYPVNKSKWNNKTS+VTPDEEIFYLVGFLFSALPTSTGK+SL+YILAQ
Sbjct: 421 KILSDSTSGPILIYPVNKSKWNNKTSLVTPDEEIFYLVGFLFSALPTSTGKESLQYILAQ 480

Query: 481 NQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQ 540
           NQ+ILDYC R L GC QYLPHY+SQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQ
Sbjct: 481 NQVILDYCARSLEGCKQYLPHYTSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQ 540

Query: 541 KATPVQHLVSKTNHKTSILEEENAKMI 568
           K T VQHL+ KT HK SILEEEN KMI
Sbjct: 541 KVTSVQHLIYKTKHKKSILEEENVKMI 567

BLAST of CmaCh14G005550 vs. TAIR 10
Match: AT2G41510.1 (cytokinin oxidase/dehydrogenase 1 )

HSP 1 Score: 716.1 bits (1847), Expect = 2.3e-206
Identity = 348/562 (61.92%), Postives = 427/562 (75.98%), Query Frame = 0

Query: 8   FLNHNKILLFKICIILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLETISLDGH 67
           F   N      I +IL ++C   RTNLC       P  + P  + S + SSL ++ L+G+
Sbjct: 9   FHRQNNKTFLGIFMILVLSCIPGRTNLCSNHSVSTPK-ELPSSNPSDIRSSLVSLDLEGY 68

Query: 68  FSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAARGHGHSL 127
            SF +    AKDFG RY + PLA+LHP SV DIS  +KHI  +GS + LTVAARGHGHSL
Sbjct: 69  ISFDDVHNVAKDFGNRYQLPPLAILHPRSVFDISSMMKHIVHLGSTSNLTVAARGHGHSL 128

Query: 128 QGQAQALRGVVINMESLKEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLTPKSWTDY 187
           QGQA A +GVVI MESL+ P ++++ G  P+VDV GGE+WINIL ETLK+GL+PKSWTDY
Sbjct: 129 QGQALAHQGVVIKMESLRSPDIRIYKGKQPYVDVSGGEIWINILRETLKYGLSPKSWTDY 188

Query: 188 LHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFYSVLGGLG 247
           LHL+VGGTLSNAGISGQAF+HGPQI+NV+QLEIVTGKGEVVTCS ++N++LF+SVLGGLG
Sbjct: 189 LHLTVGGTLSNAGISGQAFKHGPQINNVYQLEIVTGKGEVVTCSEKRNSELFFSVLGGLG 248

Query: 248 QFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFVLINRTGI 307
           QFGIITRARISLE AP MVKWIR LYSDF+ FS+DQE+LIS + +FDY+EGFV+INRT +
Sbjct: 249 QFGIITRARISLEPAPHMVKWIRVLYSDFSAFSRDQEYLISKEKTFDYVEGFVIINRTDL 308

Query: 308 LNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQLRYIPAT 367
           LNNWRSSF P D  QAS+F SD  TLYCLE+  YFN EE  +M++   KLLS+L YIP+T
Sbjct: 309 LNNWRSSFSPNDSTQASRFKSDGKTLYCLEVVKYFNPEEASSMDQETGKLLSELNYIPST 368

Query: 368 LFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFDKILSDST 427
           LF SEVPY+EFLDRVHI+E+KLR KGLWEVPHPW+NL+IP+S I  FA EVF+ IL+ + 
Sbjct: 369 LFSSEVPYIEFLDRVHIAERKLRAKGLWEVPHPWLNLLIPKSSIYQFATEVFNNILTSNN 428

Query: 428 SGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQNQIILDY 487
           +GPILIYPVN+SKW   TS++TP+E+IFYLV FL SA+P S+GK  LEY+L QNQ ++++
Sbjct: 429 NGPILIYPVNQSKWKKHTSLITPNEDIFYLVAFLPSAVPNSSGKNDLEYLLKQNQRVMNF 488

Query: 488 CVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQKAT---- 547
           C        QYLPHY +Q EW++HFG +W    +RK AYDPL+ILAPGQRIFQK T    
Sbjct: 489 CAAANLNVKQYLPHYETQKEWKSHFGKRWETFAQRKQAYDPLAILAPGQRIFQKTTGKLS 548

Query: 548 PVQHLVSKTN------HKTSIL 560
           P+Q   SK        H  SIL
Sbjct: 549 PIQLAKSKATGSPQRYHYASIL 569

BLAST of CmaCh14G005550 vs. TAIR 10
Match: AT3G63440.1 (cytokinin oxidase/dehydrogenase 6 )

HSP 1 Score: 662.9 bits (1709), Expect = 2.3e-190
Identity = 319/539 (59.18%), Postives = 405/539 (75.14%), Query Frame = 0

Query: 7   SFLNHNKILLFKICIILFINCATYRTNLCFTKCFGEPTGDPPIQSSSKLLSSLETISLDG 66
           S L    +L+ +   IL ++C  ++   CF                S  +SSL+ + L G
Sbjct: 7   SLLRKRTMLIVRSFTILLLSCIAFKLACCF----------------SSSISSLKALPLVG 66

Query: 67  HFSFKNNQAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAARGHGHS 126
           H  F++    +KDFG RY ++PLAVLHP SV DI+ T++HI+ MG+ ++LTVAARG GHS
Sbjct: 67  HLEFEHVHHASKDFGNRYQLIPLAVLHPKSVSDIASTIRHIWMMGTHSQLTVAARGRGHS 126

Query: 127 LQGQAQALRGVVINMESLKEPAMQVHS--GNLPWVDVPGGELWINILHETLKHGLTPKSW 186
           LQGQAQ   G+VI+MESL    +QV+S     P+VDV GGELWINILHETLK+GL PKSW
Sbjct: 127 LQGQAQTRHGIVIHMESLHPQKLQVYSVDSPAPYVDVSGGELWINILHETLKYGLAPKSW 186

Query: 187 TDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFYSVLG 246
           TDYLHL+VGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGE++ C+ +QN+DLF  VLG
Sbjct: 187 TDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEILNCTKRQNSDLFNGVLG 246

Query: 247 GLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTK-YSFDYIEGFVLIN 306
           GLGQFGIITRARI+LE AP MVKWIR LY DFA F+KDQE LIS + + FDYIEGFV+IN
Sbjct: 247 GLGQFGIITRARIALEPAPTMVKWIRVLYLDFAAFAKDQEQLISAQGHKFDYIEGFVIIN 306

Query: 307 RTGILNNWRSSFDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQLRY 366
           RTG+LN+WR SF  ++P++ASQF  D  TLYCLE+A Y   +  + +N+ +++ LS+L Y
Sbjct: 307 RTGLLNSWRLSFTAEEPLEASQFKFDGRTLYCLELAKYLKQDNKDVINQEVKETLSELSY 366

Query: 367 IPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFDKIL 426
           + +TLF +EV Y  FLDRVH+SE KLR KG WEVPHPW+NL++PRSKI +FA  VF  IL
Sbjct: 367 VTSTLFTTEVAYEAFLDRVHVSEVKLRSKGQWEVPHPWLNLLVPRSKINEFARGVFGNIL 426

Query: 427 SDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQNQI 486
           +D+++GP+++YPVNKSKW+N+TS VTP+EE+FYLV  L SA P S GK  +E IL +N+ 
Sbjct: 427 TDTSNGPVIVYPVNKSKWDNQTSAVTPEEEVFYLVAILTSASPGSAGKDGVEEILRRNRR 486

Query: 487 ILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQKA 543
           IL++      G  QYLPHY++++EW++HFG KW   + RKS YDPL+ILAPG RIFQKA
Sbjct: 487 ILEFSEEAGIGLKQYLPHYTTREEWRSHFGDKWGEFVRRKSRYDPLAILAPGHRIFQKA 529

BLAST of CmaCh14G005550 vs. TAIR 10
Match: AT1G75450.1 (cytokinin oxidase 5 )

HSP 1 Score: 523.5 bits (1347), Expect = 2.2e-148
Identity = 262/494 (53.04%), Postives = 351/494 (71.05%), Query Frame = 0

Query: 56  LSSLETISLDGHFSFKNNQ--AEAKDFGK-RYHMLPLAVLHPHSVRDISITLKHIFQMGS 115
           L  +  I +DGHF+   +   + + DFG  +    PLAVLHP S  D++  ++  +  GS
Sbjct: 32  LLRIGAIDVDGHFTVHPSDLASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAY--GS 91

Query: 116 GTELTVAARGHGHSLQGQAQALR-GVVINMES--LKEPAMQVHSGNLPWVDVPGGELWIN 175
            T   V+ARGHGHS+ GQA A R GVV+ M       P   V    + +VDV GGELW++
Sbjct: 92  ATAFPVSARGHGHSINGQAAAGRNGVVVEMNHGVTGTPKPLVRPDEM-YVDVWGGELWVD 151

Query: 176 ILHETLKHGLTPKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVT 235
           +L +TL+HGL PKSWTDYL+L+VGGTLSNAGISGQAF HGPQISNV +L++VTGKGEV+ 
Sbjct: 152 VLKKTLEHGLAPKSWTDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTGKGEVMR 211

Query: 236 CSAQQNADLFYSVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLIST 295
           CS ++N  LF+ VLGGLGQFGIITRARISLE AP+ V+WIR LYS F +F++DQE+LIS 
Sbjct: 212 CSEEENTRLFHGVLGGLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISM 271

Query: 296 --KYSFDYIEGFVLINRTGILNNWRSS-FDPKDPVQASQFVSDRTTLYCLEMAMYFNSEE 355
             +  FDY+EGFV+++  G++NNWRSS F P++PV+ S   S+ + LYCLE+   ++  +
Sbjct: 272 HGQLKFDYVEGFVIVDE-GLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNYHDSD 331

Query: 356 TETMNKRIEKLLSQLRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMI 415
           +E +++ +E L+ +L +IP ++F +++ YV+FLDRVH +E KLR K LWEVPHPW+NL +
Sbjct: 332 SEIVDQEVEILMKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFV 391

Query: 416 PRSKILDFAAEVFDKILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALP 475
           P+S+I DF   VF  IL + TSGPILIYP+NK KW+ ++S VTPDEE+FYLV  L SAL 
Sbjct: 392 PKSRISDFDKGVFKGILGNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALT 451

Query: 476 TSTGKQSLEYILAQNQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAY 535
                Q LEY+  QN+ IL++C +      QYLPH+++Q+EW AHFG KW      K+ +
Sbjct: 452 DGEETQKLEYLKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEF 511

Query: 536 DPLSILAPGQRIFQ 541
           DP  ILA GQRIFQ
Sbjct: 512 DPRHILATGQRIFQ 521

BLAST of CmaCh14G005550 vs. TAIR 10
Match: AT1G75450.2 (cytokinin oxidase 5 )

HSP 1 Score: 518.8 bits (1335), Expect = 5.3e-147
Identity = 262/495 (52.93%), Postives = 351/495 (70.91%), Query Frame = 0

Query: 56  LSSLETISLDGHFSFKNNQ--AEAKDFGK-RYHMLPLAVLHPHSVRDISITLKHIFQMGS 115
           L  +  I +DGHF+   +   + + DFG  +    PLAVLHP S  D++  ++  +  GS
Sbjct: 28  LLRIGAIDVDGHFTVHPSDLASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAY--GS 87

Query: 116 GTELTVAARGHGHSLQGQAQALR-GVVINMES--LKEPAMQVHSGNLPWVDVPGGELWIN 175
            T   V+ARGHGHS+ GQA A R GVV+ M       P   V    + +VDV GGELW++
Sbjct: 88  ATAFPVSARGHGHSINGQAAAGRNGVVVEMNHGVTGTPKPLVRPDEM-YVDVWGGELWVD 147

Query: 176 ILHETLKHGLTPKSWTDYLHLSVGGTLSNAGISGQAFRHGPQISNVHQLEIVT-GKGEVV 235
           +L +TL+HGL PKSWTDYL+L+VGGTLSNAGISGQAF HGPQISNV +L++VT GKGEV+
Sbjct: 148 VLKKTLEHGLAPKSWTDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTVGKGEVM 207

Query: 236 TCSAQQNADLFYSVLGGLGQFGIITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLIS 295
            CS ++N  LF+ VLGGLGQFGIITRARISLE AP+ V+WIR LYS F +F++DQE+LIS
Sbjct: 208 RCSEEENTRLFHGVLGGLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLIS 267

Query: 296 T--KYSFDYIEGFVLINRTGILNNWRSS-FDPKDPVQASQFVSDRTTLYCLEMAMYFNSE 355
              +  FDY+EGFV+++  G++NNWRSS F P++PV+ S   S+ + LYCLE+   ++  
Sbjct: 268 MHGQLKFDYVEGFVIVDE-GLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNYHDS 327

Query: 356 ETETMNKRIEKLLSQLRYIPATLFQSEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLM 415
           ++E +++ +E L+ +L +IP ++F +++ YV+FLDRVH +E KLR K LWEVPHPW+NL 
Sbjct: 328 DSEIVDQEVEILMKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLF 387

Query: 416 IPRSKILDFAAEVFDKILSDSTSGPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSAL 475
           +P+S+I DF   VF  IL + TSGPILIYP+NK KW+ ++S VTPDEE+FYLV  L SAL
Sbjct: 388 VPKSRISDFDKGVFKGILGNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSAL 447

Query: 476 PTSTGKQSLEYILAQNQIILDYCVRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSA 535
                 Q LEY+  QN+ IL++C +      QYLPH+++Q+EW AHFG KW      K+ 
Sbjct: 448 TDGEETQKLEYLKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAE 507

Query: 536 YDPLSILAPGQRIFQ 541
           +DP  ILA GQRIFQ
Sbjct: 508 FDPRHILATGQRIFQ 518

BLAST of CmaCh14G005550 vs. TAIR 10
Match: AT5G56970.1 (cytokinin oxidase 3 )

HSP 1 Score: 452.2 bits (1162), Expect = 6.1e-127
Identity = 223/473 (47.15%), Postives = 318/473 (67.23%), Query Frame = 0

Query: 74  QAEAKDFGKRYHMLPLAVLHPHSVRDISITLKHIFQMGSGTELTVAARGHGHSLQGQAQA 133
           ++ A DFG    + P AVL P SV DI+  +K  F   S     +AARGHGHS +GQA A
Sbjct: 56  ESAATDFGHVTKIFPSAVLIPSSVEDITDLIKLSFD--SQLSFPLAARGHGHSHRGQASA 115

Query: 134 LRGVVINMESL--KEPAMQVHSGNLPWVDVPGGELWINILHETLKHGLTPKSWTDYLHLS 193
             GVV+NM S+  ++  ++V S    +VDV    LWI +L++TL+ GLTP SWTDYL+L+
Sbjct: 116 KDGVVVNMRSMVNRDRGIKV-SRTCLYVDVDAAWLWIEVLNKTLELGLTPVSWTDYLYLT 175

Query: 194 VGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEVVTCSAQQNADLFYSVLGGLGQFGI 253
           VGGTLSN GISGQ FR+GPQI+NV +++++TGKGE+ TCS   N+DLF++VLGGLGQFGI
Sbjct: 176 VGGTLSNGGISGQTFRYGPQITNVLEMDVITGKGEIATCSKDMNSDLFFAVLGGLGQFGI 235

Query: 254 ITRARISLESAPKMVKWIRTLYSDFAIFSKDQEHLISTKYSFDYIEGFVLINRTGILNNW 313
           ITRARI LE APK  KW+R LY DF+ F++DQE +IS     D++EG ++++  G  +NW
Sbjct: 236 ITRARIKLEVAPKRAKWLRFLYIDFSEFTRDQERVISKTDGVDFLEGSIMVDH-GPPDNW 295

Query: 314 RSS-FDPKDPVQASQFVSDRTTLYCLEMAMYFNSEETETMNKRIEKLLSQLRYIPATLFQ 373
           RS+ + P D ++ +  V     +YCLE+  Y++     T+N+ +E+L   L ++   +++
Sbjct: 296 RSTYYPPSDHLRIASMVKRHRVIYCLEVVKYYDETSQYTVNEEMEELSDSLNHVRGFMYE 355

Query: 374 SEVPYVEFLDRVHISEKKLREKGLWEVPHPWMNLMIPRSKILDFAAEVFDKIL--SDSTS 433
            +V Y++FL+RV   E  L+ KG W+VPHPW+NL +P+++I  F   VF  I+  ++ TS
Sbjct: 356 KDVTYMDFLNRVRTGELNLKSKGQWDVPHPWLNLFVPKTQISKFDDGVFKGIILRNNITS 415

Query: 434 GPILIYPVNKSKWNNKTSVVTPDEEIFYLVGFLFSALPTSTGKQSLEYILAQNQIILDYC 493
           GP+L+YP+N++KWN++ S   P+E++FY VGFL SA     G  + E    +N  IL +C
Sbjct: 416 GPVLVYPMNRNKWNDRMSAAIPEEDVFYAVGFLRSA-----GFDNWEAFDQENMEILKFC 475

Query: 494 VRDLPGCTQYLPHYSSQDEWQAHFGSKWRVLMERKSAYDPLSILAPGQRIFQK 542
                G  QYLP++SSQ+ W  HFG +W + +ERK  YDP  IL+PGQ IFQK
Sbjct: 476 EDANMGVIQYLPYHSSQEGWVRHFGPRWNIFVERKYKYDPKMILSPGQNIFQK 519

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O222133.2e-20561.92Cytokinin dehydrogenase 1 OS=Arabidopsis thaliana OX=3702 GN=CKX1 PE=1 SV=1[more]
Q5JLP42.8e-19364.06Cytokinin dehydrogenase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX4 PE=2 ... [more]
Q75K783.8e-19063.66Cytokinin dehydrogenase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX9 PE=2 ... [more]
Q9LY713.2e-18959.18Cytokinin dehydrogenase 6 OS=Arabidopsis thaliana OX=3702 GN=CKX6 PE=2 SV=2[more]
Q67YU03.0e-14753.04Cytokinin dehydrogenase 5 OS=Arabidopsis thaliana OX=3702 GN=CKX5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1J8K40.0e+00100.00Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111482266 PE=3 SV=1[more]
A0A6J1F2R10.0e+0097.18Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111441651 PE=3 SV=1[more]
A0A1S3B8H82.2e-30289.07Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103486946 PE=3 SV=1[more]
A0A5D3DPV65.9e-29589.95Cytokinin dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A2I4G6V97.3e-22970.48Cytokinin dehydrogenase OS=Juglans regia OX=51240 GN=LOC109005247 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
XP_022983738.10.0e+00100.00cytokinin dehydrogenase 4-like [Cucurbita maxima][more]
XP_023526449.10.0e+0098.41cytokinin dehydrogenase 9-like [Cucurbita pepo subsp. pepo][more]
KAG7017671.10.0e+0097.53Cytokinin dehydrogenase 1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022934489.10.0e+0097.18cytokinin dehydrogenase 4-like [Cucurbita moschata][more]
XP_038905786.16.9e-30690.12cytokinin dehydrogenase 6-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G41510.12.3e-20661.92cytokinin oxidase/dehydrogenase 1 [more]
AT3G63440.12.3e-19059.18cytokinin oxidase/dehydrogenase 6 [more]
AT1G75450.12.2e-14853.04cytokinin oxidase 5 [more]
AT1G75450.25.3e-14752.93cytokinin oxidase 5 [more]
AT5G56970.16.1e-12747.15cytokinin oxidase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 88..231
e-value: 4.0E-17
score: 62.2
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 73..539
e-value: 1.7E-211
score: 704.8
IPR016170Cytokinin dehydrogenase, C-terminal domain superfamilyGENE3D3.40.462.10coord: 265..498
e-value: 1.7E-211
score: 704.8
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 141..264
e-value: 1.7E-211
score: 704.8
IPR015345Cytokinin dehydrogenase 1, FAD/cytokinin binding domainPFAMPF09265Cytokin-bindcoord: 263..539
e-value: 2.0E-112
score: 375.2
NoneNo IPR availablePANTHERPTHR13878:SF120CYTOKININ DEHYDROGENASE 1-LIKE ISOFORM X1coord: 12..542
NoneNo IPR availablePANTHERPTHR13878GULONOLACTONE OXIDASEcoord: 12..542
IPR006093Oxygen oxidoreductase covalent FAD-binding sitePROSITEPS00862OX2_COVAL_FADcoord: 88..123
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 84..262
score: 13.343933
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 76..259
IPR016164FAD-linked oxidase-like, C-terminalSUPERFAMILY55103FAD-linked oxidases, C-terminal domaincoord: 263..539

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh14G005550.1CmaCh14G005550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009690 cytokinin metabolic process
molecular_function GO:0019139 cytokinin dehydrogenase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity