CmaCh14G000060 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh14G000060
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	calcium-binding allergen Bet v 3-like
Location	Cma_Chr14: 40005 .. 40613 (+)
RNA-Seq Expression	CmaCh14G000060
Synteny	CmaCh14G000060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCGATGGCCTCCGATGTTCCCCGACCCCGCCTGACGAAGAAACCCTCGTCTTCCTCTTTGTCGTTCCGACTTCGGTGCCCCAGCCTGAACTCCCTTCGCCTGCGTCGCATATTTGACATGTTCGACAAGAATGGGGACGGAATGATCACCACGGAGGAGCTGGGTCAAGCTCTCGGGCTTCTTGGGCTGGATGCTGATGAATCGGACCTGGATTCCACGGTCAACTCCTTCATCGCACCGGGAAATGTTGGTCTTAGGTTCGAAGACTTTGCAGCCCTGCACCAGTCCTTAAACGACACCTATTTTGGGGAGCCGATGGAGGATGAGGAGGAGAAGGAGGAGAAGAGGGAAACAAACATGTCGCCGGTGTCGCAGGAAGAGTTGGATCTATCGGAGGCATTCAGGGTATTCGACGAGGATGGGGATGGGTACATATCCGCCACCGAACTCCAAGTGGTTCTAAGAAAGCTGAGATTGTCGGAGGGGAATGAGCTGGACAGGGTTCAGAAGATGATTTTGTCGGTTGACAGCAATCGCGATGGCCGGGTTGATTTCTTCGAATTCAAGGACATGATGCGTAGCGTTATCGTCCGGAGTTGCTGA

mRNA sequence

ATGGAAGCGATGGCCTCCGATGTTCCCCGACCCCGCCTGACGAAGAAACCCTCGTCTTCCTCTTTGTCGTTCCGACTTCGGTGCCCCAGCCTGAACTCCCTTCGCCTGCGTCGCATATTTGACATGTTCGACAAGAATGGGGACGGAATGATCACCACGGAGGAGCTGGGTCAAGCTCTCGGGCTTCTTGGGCTGGATGCTGATGAATCGGACCTGGATTCCACGGTCAACTCCTTCATCGCACCGGGAAATGTTGGTCTTAGGTTCGAAGACTTTGCAGCCCTGCACCAGTCCTTAAACGACACCTATTTTGGGGAGCCGATGGAGGATGAGGAGGAGAAGGAGGAGAAGAGGGAAACAAACATGTCGCCGGTGTCGCAGGAAGAGTTGGATCTATCGGAGGCATTCAGGGTATTCGACGAGGATGGGGATGGGTACATATCCGCCACCGAACTCCAAGTGGTTCTAAGAAAGCTGAGATTGTCGGAGGGGAATGAGCTGGACAGGGTTCAGAAGATGATTTTGTCGGTTGACAGCAATCGCGATGGCCGGGTTGATTTCTTCGAATTCAAGGACATGATGCGTAGCGTTATCGTCCGGAGTTGCTGA

Coding sequence (CDS)

ATGGAAGCGATGGCCTCCGATGTTCCCCGACCCCGCCTGACGAAGAAACCCTCGTCTTCCTCTTTGTCGTTCCGACTTCGGTGCCCCAGCCTGAACTCCCTTCGCCTGCGTCGCATATTTGACATGTTCGACAAGAATGGGGACGGAATGATCACCACGGAGGAGCTGGGTCAAGCTCTCGGGCTTCTTGGGCTGGATGCTGATGAATCGGACCTGGATTCCACGGTCAACTCCTTCATCGCACCGGGAAATGTTGGTCTTAGGTTCGAAGACTTTGCAGCCCTGCACCAGTCCTTAAACGACACCTATTTTGGGGAGCCGATGGAGGATGAGGAGGAGAAGGAGGAGAAGAGGGAAACAAACATGTCGCCGGTGTCGCAGGAAGAGTTGGATCTATCGGAGGCATTCAGGGTATTCGACGAGGATGGGGATGGGTACATATCCGCCACCGAACTCCAAGTGGTTCTAAGAAAGCTGAGATTGTCGGAGGGGAATGAGCTGGACAGGGTTCAGAAGATGATTTTGTCGGTTGACAGCAATCGCGATGGCCGGGTTGATTTCTTCGAATTCAAGGACATGATGCGTAGCGTTATCGTCCGGAGTTGCTGA

Protein sequence

MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRETNMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSNRDGRVDFFEFKDMMRSVIVRSC

Homology

[Close]

BLAST of CmaCh14G000060 vs. ExPASy Swiss-Prot
Match: P43187 (Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 9.1e-62
Identity = 126/187 (67.38%), Postives = 151/187 (80.75%), Query Frame = 0


Query: 15  KKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESDLDS 74
           +K S S+ SFRLR  SLN+LRLRRIFD+FDKN DG+IT +EL +AL LLGL+ D S+L+S
Sbjct: 20  RKRSLSNSSFRLRSESLNTLRLRRIFDLFDKNSDGIITVDELSRALNLLGLETDLSELES 79

Query: 75  TVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRETNMSPVSQEELDLSE 134
           TV SF   GN+GL+FEDF +LHQSLND+YF    EDE++ EE  +   S +SQEE D   
Sbjct: 80  TVKSFTREGNIGLQFEDFISLHQSLNDSYFAYGGEDEDDNEE--DMRKSILSQEEADSFG 139

Query: 135 AFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSNRDGRVDFFEFKDMM 194
            F+VFDEDGDGYISA ELQ+VL KL  SEG+E+DRV+KMI+SVDSNRDGRVDFFEFKDMM
Sbjct: 140 GFKVFDEDGDGYISARELQMVLGKLGFSEGSEIDRVEKMIVSVDSNRDGRVDFFEFKDMM 199

Query: 195 RSVIVRS 202
           RSV+VRS
Sbjct: 200 RSVLVRS 204

[Close]

BLAST of CmaCh14G000060 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 1.5e-56
Identity = 114/187 (60.96%), Postives = 147/187 (78.61%), Query Frame = 0


Query: 15  KKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESDLDS 74
           KK +  S SFRLR PSLN+LRL+RIFD+FDKNGDG IT EEL QAL  LGL+AD SDL S
Sbjct: 9   KKVARQSSSFRLRSPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKS 68

Query: 75  TVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRETNMSPVSQEELDLSE 134
           TV S+I PGN GL F+DF++LH++L+D++FG      E +++      S  ++ E DL+E
Sbjct: 69  TVESYIQPGNTGLNFDDFSSLHKTLDDSFFGGACGGGENEDDP-----SSAAENESDLAE 128

Query: 135 AFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSNRDGRVDFFEFKDMM 194
           AF+VFDE+GDG+ISA ELQ VL+KL L EG E++RV+KMI+SVD N+DGRVDFFEFK+MM
Sbjct: 129 AFKVFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMM 188

Query: 195 RSVIVRS 202
           R+V++ S
Sbjct: 189 RTVVIPS 190

[Close]

BLAST of CmaCh14G000060 vs. ExPASy Swiss-Prot
Match: Q09011 (Calcium-binding protein CAST OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 1.3e-55
Identity = 121/186 (65.05%), Postives = 141/186 (75.81%), Query Frame = 0


Query: 16  KPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESDLDST 75
           KPSSSS SFRLR PSLNS+RLRRIFD+FD+N D +I+ EEL QAL LLGLDAD S+++S 
Sbjct: 22  KPSSSS-SFRLRSPSLNSIRLRRIFDVFDRNHDCLISVEELSQALNLLGLDADLSEIESM 81

Query: 76  VNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRETNMSPVSQEELDLSEA 135
           V   I P N GLRFEDF  LH+SLND +FG   ED      K   N  P +Q+E DL EA
Sbjct: 82  VKLHIKPENTGLRFEDFETLHRSLNDVFFGSKCED------KLGLNPDP-AQDESDLKEA 141

Query: 136 FRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSNRDGRVDFFEFKDMMR 195
           F VFDE+GDG+ISA ELQVVL KL L EG+E+DRV+ MI SV+ + DGRVDFFEFKDMMR
Sbjct: 142 FDVFDENGDGFISAKELQVVLEKLGLPEGSEIDRVEMMISSVEQDHDGRVDFFEFKDMMR 199

Query: 196 SVIVRS 202
           +VIV S
Sbjct: 202 TVIVPS 199

[Close]

BLAST of CmaCh14G000060 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 7.0e-54
Identity = 116/187 (62.03%), Postives = 139/187 (74.33%), Query Frame = 0


Query: 15  KKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESDLDS 74
           KK S  S SFRLR PSLN+LRL R+FD+FDKN DG IT EEL QAL  LGLDAD SDL S
Sbjct: 8   KKLSRQSSSFRLRSPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKS 67

Query: 75  TVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRETNMSPVSQEELDLSE 134
           TV+SFI P   GLRF+DFAALH++L++++FG          E    + SP    E DL E
Sbjct: 68  TVDSFIKPDKTGLRFDDFAALHKTLDESFFG---------GEGSCCDGSP----ESDLEE 127

Query: 135 AFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSNRDGRVDFFEFKDMM 194
           AF VFDEDGDG+ISA ELQ VL+KL L E  E+++V+KMI+SVDSN DGRVDFFEFK+MM
Sbjct: 128 AFNVFDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 181

Query: 195 RSVIVRS 202
           ++V+V S
Sbjct: 188 QTVVVPS 181

[Close]

BLAST of CmaCh14G000060 vs. ExPASy Swiss-Prot
Match: Q10LX4 (Probable calcium-binding protein CML27 OS=Oryza sativa subsp. japonica OX=39947 GN=CML27 PE=2 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 3.5e-45
Identity = 103/190 (54.21%), Postives = 132/190 (69.47%), Query Frame = 0


Query: 10  RPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADE 69
           +P L++KPS    SFRLR  SLN+LRLRR+FD+FD+NGDG IT +E+  AL  LGL AD 
Sbjct: 9   KPSLSRKPSP---SFRLRNGSLNALRLRRVFDLFDRNGDGEITLDEMASALDALGLGADR 68

Query: 70  SDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRETNMSPVSQEE 129
           + L++TV  +I  G  GLRF DF ALH++L D  FG P+E+EE  ++  +        +E
Sbjct: 69  AGLEATVGGYIPAGAAGLRFGDFEALHRALGDALFG-PVEEEEPGKQGED-------DDE 128

Query: 130 LDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSNRDGRVDFFE 189
            D+ EAFRVFDEDGDG+ISA ELQ VL+KL L E   L  VQ+MI +VD + DGRVDF E
Sbjct: 129 GDMKEAFRVFDEDGDGFISAAELQAVLKKLGLPEARNLATVQEMICNVDRDCDGRVDFGE 187

Query: 190 FKDMMRSVIV 200
           FK MM+ + V
Sbjct: 189 FKCMMQGITV 187

[Close]

BLAST of CmaCh14G000060 vs. ExPASy TrEMBL
Match: A0A6J1J521 (calcium-binding allergen Bet v 3-like OS=Cucurbita maxima OX=3661 GN=LOC111481430 PE=4 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 2.4e-105
Identity = 202/202 (100.00%), Postives = 202/202 (100.00%), Query Frame = 0


Query: 1   MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL
Sbjct: 1   MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60

Query: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET 120
           GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET
Sbjct: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET 120

Query: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180
           NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN
Sbjct: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180

Query: 181 RDGRVDFFEFKDMMRSVIVRSC 203
           RDGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RDGRVDFFEFKDMMRSVIVRSC 202

[Close]

BLAST of CmaCh14G000060 vs. ExPASy TrEMBL
Match: A0A6J1F9I1 (calcium-binding allergen Bet v 3-like OS=Cucurbita moschata OX=3662 GN=LOC111442046 PE=4 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 8.4e-103
Identity = 198/202 (98.02%), Postives = 200/202 (99.01%), Query Frame = 0


Query: 1   MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEA+ASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60

Query: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET 120
           GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQ LNDTYF EP+EDEEEKEEKRET
Sbjct: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQFLNDTYFREPVEDEEEKEEKRET 120

Query: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180
           NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN
Sbjct: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180

Query: 181 RDGRVDFFEFKDMMRSVIVRSC 203
           RDGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RDGRVDFFEFKDMMRSVIVRSC 202

[Close]

BLAST of CmaCh14G000060 vs. ExPASy TrEMBL
Match: A0A0A0LA10 (Calcium binding protein/cast OS=Cucumis sativus OX=3659 GN=Csa_3G727960 PE=4 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 2.3e-92
Identity = 181/202 (89.60%), Postives = 192/202 (95.05%), Query Frame = 0


Query: 1   MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEA+ASDVPRPRLTKKPSS S SFRLRCPSLNSLRLRRIFDMFDKNGDGMITT+ELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSLSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTDELGQAL 60

Query: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET 120
           GLLGLDADES+LDSTVNSFI PGN GLRFEDFAALHQSLNDTYFG  +EDE+E +EKRET
Sbjct: 61  GLLGLDADESELDSTVNSFIEPGNEGLRFEDFAALHQSLNDTYFGGMVEDEDE-DEKRET 120

Query: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180
           +MS +SQEE DLSEAFRVFDEDGDGYISATELQVVLRKLRL+EGNE+DRVQKMILSVD N
Sbjct: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN 180

Query: 181 RDGRVDFFEFKDMMRSVIVRSC 203
           +DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 QDGRVDFFEFKDMMRSVIVRSC 201

[Close]

BLAST of CmaCh14G000060 vs. ExPASy TrEMBL
Match: A0A5A7TQ56 (Calcium-binding allergen Bet v 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00090 PE=4 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 5.1e-92
Identity = 181/202 (89.60%), Postives = 190/202 (94.06%), Query Frame = 0


Query: 1   MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEA+ASDVPRPRLTKKPSSSS SFRLRCPSLNSLRLRRIFDMFDKNGDGMIT EELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITAEELGQAL 60

Query: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET 120
           GLLGLDADES+LDSTVNSFI PGNVGLRFEDFAALHQSLNDTYF   +EDE   +EKRET
Sbjct: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDE---DEKRET 120

Query: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180
           +MS +SQEE DLSEAFRVFDEDGDGYISATELQVVLRKLRL+EGNE+DRVQKMILSVD N
Sbjct: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN 180

Query: 181 RDGRVDFFEFKDMMRSVIVRSC 203
           +DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 QDGRVDFFEFKDMMRSVIVRSC 199

[Close]

BLAST of CmaCh14G000060 vs. ExPASy TrEMBL
Match: A0A1S3B620 (calcium-binding allergen Bet v 3 OS=Cucumis melo OX=3656 GN=LOC103486221 PE=4 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 5.1e-92
Identity = 181/202 (89.60%), Postives = 190/202 (94.06%), Query Frame = 0


Query: 1   MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEA+ASDVPRPRLTKKPSSSS SFRLRCPSLNSLRLRRIFDMFDKNGDGMIT EELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITAEELGQAL 60

Query: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET 120
           GLLGLDADES+LDSTVNSFI PGNVGLRFEDFAALHQSLNDTYF   +EDE   +EKRET
Sbjct: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDE---DEKRET 120

Query: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180
           +MS +SQEE DLSEAFRVFDEDGDGYISATELQVVLRKLRL+EGNE+DRVQKMILSVD N
Sbjct: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN 180

Query: 181 RDGRVDFFEFKDMMRSVIVRSC 203
           +DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 QDGRVDFFEFKDMMRSVIVRSC 199

[Close]

BLAST of CmaCh14G000060 vs. NCBI nr
Match: XP_022982604.1 (calcium-binding allergen Bet v 3-like [Cucurbita maxima])

HSP 1 Score: 391.3 bits (1004), Expect = 4.9e-105
Identity = 202/202 (100.00%), Postives = 202/202 (100.00%), Query Frame = 0


Query: 1   MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL
Sbjct: 1   MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60

Query: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET 120
           GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET
Sbjct: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET 120

Query: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180
           NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN
Sbjct: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180

Query: 181 RDGRVDFFEFKDMMRSVIVRSC 203
           RDGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RDGRVDFFEFKDMMRSVIVRSC 202

[Close]

BLAST of CmaCh14G000060 vs. NCBI nr
Match: XP_023527056.1 (calcium-binding allergen Bet v 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 388.3 bits (996), Expect = 4.1e-104
Identity = 200/202 (99.01%), Postives = 202/202 (100.00%), Query Frame = 0


Query: 1   MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEA+ASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL
Sbjct: 70  MEAVASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 129

Query: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET 120
           GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEP+EDEEEKEEKRET
Sbjct: 130 GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPVEDEEEKEEKRET 189

Query: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180
           NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN
Sbjct: 190 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 249

Query: 181 RDGRVDFFEFKDMMRSVIVRSC 203
           RDGRVDFFEFKDMMRSVIVRSC
Sbjct: 250 RDGRVDFFEFKDMMRSVIVRSC 271

[Close]

BLAST of CmaCh14G000060 vs. NCBI nr
Match: KAG6580420.1 (Calcium-binding allergen Bet v 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 385.2 bits (988), Expect = 3.5e-103
Identity = 199/202 (98.51%), Postives = 201/202 (99.50%), Query Frame = 0


Query: 1   MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEA+ASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60

Query: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET 120
           GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYF EP+EDEEEKEEKRET
Sbjct: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFREPVEDEEEKEEKRET 120

Query: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180
           NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN
Sbjct: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180

Query: 181 RDGRVDFFEFKDMMRSVIVRSC 203
           RDGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RDGRVDFFEFKDMMRSVIVRSC 202

[Close]

BLAST of CmaCh14G000060 vs. NCBI nr
Match: XP_022935065.1 (calcium-binding allergen Bet v 3-like [Cucurbita moschata])

HSP 1 Score: 382.9 bits (982), Expect = 1.7e-102
Identity = 198/202 (98.02%), Postives = 200/202 (99.01%), Query Frame = 0


Query: 1   MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEA+ASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60

Query: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET 120
           GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQ LNDTYF EP+EDEEEKEEKRET
Sbjct: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQFLNDTYFREPVEDEEEKEEKRET 120

Query: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180
           NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN
Sbjct: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180

Query: 181 RDGRVDFFEFKDMMRSVIVRSC 203
           RDGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RDGRVDFFEFKDMMRSVIVRSC 202

[Close]

BLAST of CmaCh14G000060 vs. NCBI nr
Match: XP_004137668.1 (calcium-binding protein CML42 [Cucumis sativus] >KGN58678.1 hypothetical protein Csa_002212 [Cucumis sativus])

HSP 1 Score: 348.2 bits (892), Expect = 4.7e-92
Identity = 181/202 (89.60%), Postives = 192/202 (95.05%), Query Frame = 0


Query: 1   MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60
           MEA+ASDVPRPRLTKKPSS S SFRLRCPSLNSLRLRRIFDMFDKNGDGMITT+ELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSLSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTDELGQAL 60

Query: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET 120
           GLLGLDADES+LDSTVNSFI PGN GLRFEDFAALHQSLNDTYFG  +EDE+E +EKRET
Sbjct: 61  GLLGLDADESELDSTVNSFIEPGNEGLRFEDFAALHQSLNDTYFGGMVEDEDE-DEKRET 120

Query: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180
           +MS +SQEE DLSEAFRVFDEDGDGYISATELQVVLRKLRL+EGNE+DRVQKMILSVD N
Sbjct: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRN 180

Query: 181 RDGRVDFFEFKDMMRSVIVRSC 203
           +DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 QDGRVDFFEFKDMMRSVIVRSC 201

[Close]

BLAST of CmaCh14G000060 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 220.7 bits (561), Expect = 1.1e-57
Identity = 114/187 (60.96%), Postives = 147/187 (78.61%), Query Frame = 0


Query: 15  KKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESDLDS 74
           KK +  S SFRLR PSLN+LRL+RIFD+FDKNGDG IT EEL QAL  LGL+AD SDL S
Sbjct: 9   KKVARQSSSFRLRSPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKS 68

Query: 75  TVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRETNMSPVSQEELDLSE 134
           TV S+I PGN GL F+DF++LH++L+D++FG      E +++      S  ++ E DL+E
Sbjct: 69  TVESYIQPGNTGLNFDDFSSLHKTLDDSFFGGACGGGENEDDP-----SSAAENESDLAE 128

Query: 135 AFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSNRDGRVDFFEFKDMM 194
           AF+VFDE+GDG+ISA ELQ VL+KL L EG E++RV+KMI+SVD N+DGRVDFFEFK+MM
Sbjct: 129 AFKVFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMM 188

Query: 195 RSVIVRS 202
           R+V++ S
Sbjct: 189 RTVVIPS 190

[Close]

BLAST of CmaCh14G000060 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 211.8 bits (538), Expect = 4.9e-55
Identity = 116/187 (62.03%), Postives = 139/187 (74.33%), Query Frame = 0


Query: 15  KKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESDLDS 74
           KK S  S SFRLR PSLN+LRL R+FD+FDKN DG IT EEL QAL  LGLDAD SDL S
Sbjct: 8   KKLSRQSSSFRLRSPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKS 67

Query: 75  TVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRETNMSPVSQEELDLSE 134
           TV+SFI P   GLRF+DFAALH++L++++FG          E    + SP    E DL E
Sbjct: 68  TVDSFIKPDKTGLRFDDFAALHKTLDESFFG---------GEGSCCDGSP----ESDLEE 127

Query: 135 AFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSNRDGRVDFFEFKDMM 194
           AF VFDEDGDG+ISA ELQ VL+KL L E  E+++V+KMI+SVDSN DGRVDFFEFK+MM
Sbjct: 128 AFNVFDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 181

Query: 195 RSVIVRS 202
           ++V+V S
Sbjct: 188 QTVVVPS 181

[Close]

BLAST of CmaCh14G000060 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 112.8 bits (281), Expect = 3.1e-25
Identity = 71/179 (39.66%), Postives = 102/179 (56.98%), Query Frame = 0


Query: 17  PSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESDLDSTV 76
           PSSSS   +   PS     L+R+F MFDKNGDG IT EEL  +L  LG+   + DL   +
Sbjct: 51  PSSSSAPTKRIDPS----ELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMI 110

Query: 77  NSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRETNMSPVSQEELDLSEAF 136
           +   A G+  +  ++F +L+ S+ D    E   D E +EE              D+ +AF
Sbjct: 111 HKIDANGDGCVDIDEFESLYSSIVD----EHHNDGETEEE--------------DMKDAF 170

Query: 137 RVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSNRDGRVDFFEFKDMMR 196
            VFD+DGDG+I+  EL+ V+  L L +G  LD  +KMI+ VD++ DGRV++ EF  MM+
Sbjct: 171 NVFDQDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKEFLQMMK 207

[Close]

BLAST of CmaCh14G000060 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 108.2 bits (269), Expect = 7.7e-24
Identity = 61/165 (36.97%), Postives = 95/165 (57.58%), Query Frame = 0


Query: 31  LNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESDLDSTVNSFIAPGNVGLRFE 90
           ++S  L R+F MFDK+GDG ITT+EL ++   LG+   E +L   +      G+  +  E
Sbjct: 1   MDSTELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIE 60

Query: 91  DFAALHQSLNDTYFGEPMEDEEEKEEKRETNMSPVSQEELDLSEAFRVFDEDGDGYISAT 150
           +F  L++++        +EDE+E  E+             D+ EAF VFD +GDG+I+  
Sbjct: 61  EFGELYKTI-------MVEDEDEVGEE-------------DMKEAFNVFDRNGDGFITVD 120

Query: 151 ELQVVLRKLRLSEGNELDRVQKMILSVDSNRDGRVDFFEFKDMMR 196
           EL+ VL  L L +G  L+  +KMI+ VD + DGRV++ EF+ MM+
Sbjct: 121 ELKAVLSSLGLKQGKTLEECRKMIMQVDVDGDGRVNYMEFRQMMK 145

[Close]

BLAST of CmaCh14G000060 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 107.1 bits (266), Expect = 1.7e-23
Identity = 60/165 (36.36%), Postives = 95/165 (57.58%), Query Frame = 0


Query: 31  LNSLRLRRIFDMFDKNGDGMITTEELGQALGLLGLDADESDLDSTVNSFIAPGNVGLRFE 90
           ++   L+R+F MFDKNGDG IT +EL + L  LG+   + +L   +      G+  +  +
Sbjct: 1   MDPTELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDID 60

Query: 91  DFAALHQSLNDTYFGEPMEDEEEKEEKRETNMSPVSQEELDLSEAFRVFDEDGDGYISAT 150
           +F  L++++        M++E+E+EE              D+ EAF VFD++GDG+I+  
Sbjct: 61  EFGELYKTI--------MDEEDEEEE--------------DMKEAFNVFDQNGDGFITVD 120

Query: 151 ELQVVLRKLRLSEGNELDRVQKMILSVDSNRDGRVDFFEFKDMMR 196
           EL+ VL  L L +G  LD  +KMI  VD + DGRV++ EF+ MM+
Sbjct: 121 ELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

The following BLAST results are available for this feature:

BLAST of CmaCh14G000060 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43187	9.1e-62	67.38	Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1	[more]
Q9SVG9	1.5e-56	60.96	Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1	[more]
Q09011	1.3e-55	65.05	Calcium-binding protein CAST OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]
Q9FI19	7.0e-54	62.03	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ...	[more]
Q10LX4	3.5e-45	54.21	Probable calcium-binding protein CML27 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of CmaCh14G000060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J521	2.4e-105	100.00	calcium-binding allergen Bet v 3-like OS=Cucurbita maxima OX=3661 GN=LOC11148143...	[more]
A0A6J1F9I1	8.4e-103	98.02	calcium-binding allergen Bet v 3-like OS=Cucurbita moschata OX=3662 GN=LOC111442...	[more]
A0A0A0LA10	2.3e-92	89.60	Calcium binding protein/cast OS=Cucumis sativus OX=3659 GN=Csa_3G727960 PE=4 SV=...	[more]
A0A5A7TQ56	5.1e-92	89.60	Calcium-binding allergen Bet v 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3B620	5.1e-92	89.60	calcium-binding allergen Bet v 3 OS=Cucumis melo OX=3656 GN=LOC103486221 PE=4 SV...	[more]

BLAST of CmaCh14G000060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022982604.1	4.9e-105	100.00	calcium-binding allergen Bet v 3-like [Cucurbita maxima]	[more]
XP_023527056.1	4.1e-104	99.01	calcium-binding allergen Bet v 3-like [Cucurbita pepo subsp. pepo]	[more]
KAG6580420.1	3.5e-103	98.51	Calcium-binding allergen Bet v 3, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022935065.1	1.7e-102	98.02	calcium-binding allergen Bet v 3-like [Cucurbita moschata]	[more]
XP_004137668.1	4.7e-92	89.60	calcium-binding protein CML42 [Cucumis sativus] >KGN58678.1 hypothetical protein...	[more]

BLAST of CmaCh14G000060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20780.1	1.1e-57	60.96	calmodulin like 42	[more]
AT5G44460.1	4.9e-55	62.03	calmodulin like 43	[more]
AT2G43290.1	3.1e-25	39.66	Calcium-binding EF-hand family protein	[more]
AT4G03290.1	7.7e-24	36.97	EF hand calcium-binding protein family	[more]
AT1G05990.1	1.7e-23	36.36	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 35..63 e-value: 2.4E-6 score: 37.1 coord: 131..159 e-value: 2.8E-6 score: 36.9 coord: 169..197 e-value: 1.3E-5 score: 34.6
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 35..64 e-value: 1.8E-8 score: 33.5
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 132..195 e-value: 6.1E-14 score: 52.2
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 127..162 score: 14.652882
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 165..200 score: 13.062845
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 31..66 score: 15.712908
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 131..195 e-value: 1.12736E-16 score: 69.1137
None	No IPR available	GENE3D	1.10.238.10		coord: 33..101 e-value: 2.7E-13 score: 51.8
None	No IPR available	GENE3D	1.10.238.10		coord: 161..201 e-value: 2.0E-6 score: 29.8
None	No IPR available	GENE3D	1.10.238.10		coord: 123..160 e-value: 3.4E-11 score: 44.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 103..126
None	No IPR available	PANTHER	PTHR10891:SF743	CALCIUM-BINDING PROTEIN CML42	coord: 5..201
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 5..201
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 44..56
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 178..190
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 140..152
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 35..195

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh14G000060.1	CmaCh14G000060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding