CmaCh13G011410 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh13G011410
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionProtein of unknown function (DUF962)
LocationCma_Chr13: 8421124 .. 8423804 (+)
RNA-Seq ExpressionCmaCh13G011410
SyntenyCmaCh13G011410
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGTGAATTGAGAAATAAAAGAAAGAAATAGATATGAGCGACCAAAATGTAGAGCAATAGTTTGAAGAACAGCTATGAATTGAGATGGTCAATGGAATTTGGGAAAGGAAGGATCCAATCCAATCCAAATCCAAATCGGAGCTCACCTTTTCACACCGGCGCATCTTAGCAATCCACGCGACGTTGAACAGTGGAAGCCGTCCTGAAATTTCAATTCCCAATTCCAACGCGGCGCCAAACAAGTCCAAATCTTCCAAATTTGACGCCAAACCTATGCACCATCCCACACTTGGATTCAATATATTTCGGTGTGCCCTCCCGCTCTCCTTTTTCGCAATCCCATATCCTCATCCTTCCTTTTATACCCGATTGCCTCTCCACTTGTTCCTCGCACCACTTCCATTTTCACTATTTCTTTCTGTATTTCTCCTTACATTCAATGGGTAGGCGCGGATTGTTCGACCTGGAGAGGCATTTTGCGTTCTATGGGGCTTATCATAGCAACCCAACAAATATATTTATCCATATTCTCTTCGTCTGGCCAATATTCTTCACTGCTCTCATGCTCTTCTACTACACCCCTTCCTTCTATTCTTTTCCCAAATGCCCTTGCGGCTTCAATACTGGCCTCGTTGTGAATTTTGGTTCTTTGTTTGCTTTGGTGTATGCCGTTTTCTACGTACTTTTTGATAAGAGAGCTGGATCTCTGGCTGCTTTTATGTGTTTTCTCTGCTGGGTTGGAGCAAGCGTTTTGGCTTTTAAACTTGGATGGACGCTTACTTGGAAGGTAGACGCTAAACCCCCCCTTTTTTTCCCTTCCTCTTTTGATCCTTTTTTGTTTGTTTTATTCTTTGTCGGGTAGGAATCACAATTTAATACTTCACAGATCTTATAATAACCCTATTTAGGTTGTCTGGTTTCTGCTCCAATTCAATTATTCGAACCATTTATGAATCTCAGTTCCCTGTAGTTCTATTCAATTTCTTATGGGGCCGGAACTCATAGTTTTGGTCTGATGTATGAAGGGCTGGCCATGGTGTTGACATAGCTTCTATTGAATTTATGGTTGTACTAGACTACAATTTATTTCTCTTGGTACTGTAGGACAGGTTTCATTTTCGTAGAGGAGAAAGCTTAGTGTGGCATGAAAAAAATAAAATTAGAAAGAAGAAAGATATTTGTTCGAGCTTCTTCCAACTTTATGGTGGTTGTGGGAATTTACTGCTCCTATTTATCCTTCTTTTTTCAGTTTATAAAGATTGTGCTGCCAAATTCATTTGGTCTCGAACCATGAATATCTCAGCTCTTCGTTTAGGCATGTTTGAGAGTAGGTTTGATATGGTTCACTTTGAAAACCCTCCCAAGACATGCTTTTAACCATTTAAGATAAATTTTGATGGTATGAAAATTTGCATTTAAAAGTGGAAAACCAAATATTAAATTGATTTCGAATCATCAACAACATGTTTTACAATGGTTTTAAACATGATAGAAGTGATTTTAACCTTTCAAAATCGCTTATCAAACTCCATGTAACTTCTATCTTTATCATCAACACATTTTCTTGCTATTCTGTTTATATGATGGTGGTTTCTGGTTTTGAAGTTAAAGCAAAGCTAGTTAGGCCAGGTTTAATAACACTTTAATTTGGCAGATGTCTACAAGATTTTCTTAGAAGTTAGAATACTGGTCGACGTTGCTTTCCTTGGATCTGAGTCATCCTTTTGTCGTCCTCATCACATCTCCTTTTCCCTTCCATACAATTTTTTGAGAGGCTTGACAGCATGTTAATGTAATTGTAGTCGGCTAAACTTGATGGACATCAGTGTTGAGGACATTTTTATCCTCTACCTCAACTACTTTTTGTAAGAACTATCTCAAAGGCTTGTGACACATATTTGGGATGAGAAAGCTTGCAGCTTTTGAATTTTGGATCAGTTTGGAGATTCCTTTTGGATAATGTAATTTAAGGCCACCTTTTGAAAAAAAGTCACCACCACTACATTATATGATTCACCGTTTCCTTTTCTTCTTACGACATCATAGTAACTTTGACAGCCACAACCATTTATGTGTTTGTTCGAGTACTCCTTGGCATATTTGGACCCCGTCATTCCATGTCCATAAAGTTTTGAGTACTACTTTGCAAATTATAACTTGTAGCAAACCTAAGGAATCTACAGAACATGATCAAGTTATTGCAGAATCTATAGTCTTGAGCATAAAGCTAAAGTTGGTTATTTTGTAATTTGATATACCAGGTGGTGTTGGCTGCACAGTTGTTCTGCTGGACAAGTCAGTTTATTGGCCATGGGGTGTTCGAGGTAACTAGTTAGTTAAACATCTTAAACATGAACTTGTTTTGCAACCTTAATTCCTGATGGGTTTTGGTTTGATTTTGTAGAAACGGGCACCTGCTCTCATTGACAACCTTGCCCAAGCTTTTCTAATGGCTCCATTCTTCGTTTTGTTGGAGGTTACCTTTGCTGAAACCCCTCCTTGTGCCCTTCTTGGATCAACAATGAAACATTTGAAAATGTAACTAATTTGTTAACTATTTGCAGATTCTTCAAGCTTCGTTCAAATATGAACCATACAATGGATTTCACGCAAGCGTGAAGGCGAAGATGGAAGCCGAGAAGAAAGCTTGGGAAGGAAAGAAAGAAAAGAAAAGCTGTTAG

mRNA sequence

TGGTGAATTGAGAAATAAAAGAAAGAAATAGATATGAGCGACCAAAATGTAGAGCAATAGTTTGAAGAACAGCTATGAATTGAGATGGTCAATGGAATTTGGGAAAGGAAGGATCCAATCCAATCCAAATCCAAATCGGAGCTCACCTTTTCACACCGGCGCATCTTAGCAATCCACGCGACGTTGAACAGTGGAAGCCGTCCTGAAATTTCAATTCCCAATTCCAACGCGGCGCCAAACAAGTCCAAATCTTCCAAATTTGACGCCAAACCTATGCACCATCCCACACTTGGATTCAATATATTTCGGTGTGCCCTCCCGCTCTCCTTTTTCGCAATCCCATATCCTCATCCTTCCTTTTATACCCGATTGCCTCTCCACTTGTTCCTCGCACCACTTCCATTTTCACTATTTCTTTCTGTATTTCTCCTTACATTCAATGGGTAGGCGCGGATTGTTCGACCTGGAGAGGCATTTTGCGTTCTATGGGGCTTATCATAGCAACCCAACAAATATATTTATCCATATTCTCTTCGTCTGGCCAATATTCTTCACTGCTCTCATGCTCTTCTACTACACCCCTTCCTTCTATTCTTTTCCCAAATGCCCTTGCGGCTTCAATACTGGCCTCGTTGTGAATTTTGGTTCTTTGTTTGCTTTGGTGTATGCCGTTTTCTACGTACTTTTTGATAAGAGAGCTGGATCTCTGGCTGCTTTTATGTGTTTTCTCTGCTGGGTTGGAGCAAGCGTTTTGGCTTTTAAACTTGGATGGACGCTTACTTGGAAGGTGGTGTTGGCTGCACAGTTGTTCTGCTGGACAAGTCAGTTTATTGGCCATGGGGTGTTCGAGAAACGGGCACCTGCTCTCATTGACAACCTTGCCCAAGCTTTTCTAATGGCTCCATTCTTCGTTTTGTTGGAGATTCTTCAAGCTTCGTTCAAATATGAACCATACAATGGATTTCACGCAAGCGTGAAGGCGAAGATGGAAGCCGAGAAGAAAGCTTGGGAAGGAAAGAAAGAAAAGAAAAGCTGTTAG

Coding sequence (CDS)

ATGGGTAGGCGCGGATTGTTCGACCTGGAGAGGCATTTTGCGTTCTATGGGGCTTATCATAGCAACCCAACAAATATATTTATCCATATTCTCTTCGTCTGGCCAATATTCTTCACTGCTCTCATGCTCTTCTACTACACCCCTTCCTTCTATTCTTTTCCCAAATGCCCTTGCGGCTTCAATACTGGCCTCGTTGTGAATTTTGGTTCTTTGTTTGCTTTGGTGTATGCCGTTTTCTACGTACTTTTTGATAAGAGAGCTGGATCTCTGGCTGCTTTTATGTGTTTTCTCTGCTGGGTTGGAGCAAGCGTTTTGGCTTTTAAACTTGGATGGACGCTTACTTGGAAGGTGGTGTTGGCTGCACAGTTGTTCTGCTGGACAAGTCAGTTTATTGGCCATGGGGTGTTCGAGAAACGGGCACCTGCTCTCATTGACAACCTTGCCCAAGCTTTTCTAATGGCTCCATTCTTCGTTTTGTTGGAGATTCTTCAAGCTTCGTTCAAATATGAACCATACAATGGATTTCACGCAAGCGTGAAGGCGAAGATGGAAGCCGAGAAGAAAGCTTGGGAAGGAAAGAAAGAAAAGAAAAGCTGTTAG

Protein sequence

MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGFNTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLAAQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVKAKMEAEKKAWEGKKEKKSC
Homology
BLAST of CmaCh13G011410 vs. ExPASy Swiss-Prot
Match: O13737 (2-hydroxy-palmitic acid dioxygenase mpo1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mpo1 PE=3 SV=2)

HSP 1 Score: 93.6 bits (231), Expect = 2.7e-18
Identity = 66/181 (36.46%), Postives = 92/181 (50.83%), Query Frame = 0

Query: 9   LERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGFNTGLVVNF 68
           L R ++FY AYHSNP NI IH + +  +  TAL+L +               N+ L +N 
Sbjct: 4   LSRSYSFYAAYHSNPVNIKIHQVCIPLLLLTALVLLHNFVITL--------INSKLQINV 63

Query: 69  GSLFALVYAVFYVLFDKRAGSLAAFMCFL-CWVGASVLAFKLGWTLTWKVVLAAQLFCWT 128
             L  L Y +FYV  D   G L + + +L  ++  S L      +L  +      + CW 
Sbjct: 64  AHLVGLAYQIFYVTLDPLDGLLYSPVLYLFSYILPSKLFTIFSRSLVNRSAAVVHVICWI 123

Query: 129 SQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVKAKMEAEK 188
            QFIGHGVFEKR PAL+DNL Q+  +AP F  LE         P+ G++ SV +K+ A  
Sbjct: 124 LQFIGHGVFEKRKPALLDNLIQSLFIAPLFAFLET-------GPFVGYYPSVVSKIRANI 169

BLAST of CmaCh13G011410 vs. ExPASy Swiss-Prot
Match: P25338 (2-hydroxy-palmitic acid dioxygenase MPO1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MPO1 PE=1 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 2.0e-16
Identity = 60/180 (33.33%), Postives = 93/180 (51.67%), Query Frame = 0

Query: 5   GLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGFNTGL 64
           GL DL     FY  YH NP N+ IH +FV  I F+   + +    + S            
Sbjct: 4   GLLDLRSQLGFYKFYHHNPKNVLIHSIFVPTILFSGSCMLHRVKIYQS------------ 63

Query: 65  VVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLAAQLF 124
            ++  ++ ++++++FY L     G LA  +  L  +  +++  ++   LT+K  L     
Sbjct: 64  -ISLTAVLSVLFSIFYCLLYLPTGLLAGVLLLL--LNLALIDHRV--DLTFKQELGLFTI 123

Query: 125 CWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVKAKME 184
            W  QF+GHGVFEKR PALIDNL Q+ ++AP+F++ E L   FK     GF   +KA +E
Sbjct: 124 GWIFQFVGHGVFEKRRPALIDNLVQSLVLAPYFIMFEFL---FKL----GFMPRLKATLE 159

BLAST of CmaCh13G011410 vs. ExPASy TrEMBL
Match: A0A6J1KEF1 (uncharacterized endoplasmic reticulum membrane protein YGL010W-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495075 PE=4 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 1.2e-109
Identity = 199/199 (100.00%), Postives = 199/199 (100.00%), Query Frame = 0

Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF
Sbjct: 109 MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 168

Query: 61  NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA 120
           NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA
Sbjct: 169 NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA 228

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK
Sbjct: 229 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK 288

Query: 181 AKMEAEKKAWEGKKEKKSC 200
           AKMEAEKKAWEGKKEKKSC
Sbjct: 289 AKMEAEKKAWEGKKEKKSC 307

BLAST of CmaCh13G011410 vs. ExPASy TrEMBL
Match: A0A6J1KKR6 (uncharacterized endoplasmic reticulum membrane protein YGL010W-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495075 PE=4 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 1.2e-109
Identity = 199/199 (100.00%), Postives = 199/199 (100.00%), Query Frame = 0

Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF
Sbjct: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60

Query: 61  NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA 120
           NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA
Sbjct: 61  NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA 120

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK
Sbjct: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK 180

Query: 181 AKMEAEKKAWEGKKEKKSC 200
           AKMEAEKKAWEGKKEKKSC
Sbjct: 181 AKMEAEKKAWEGKKEKKSC 199

BLAST of CmaCh13G011410 vs. ExPASy TrEMBL
Match: A0A6J1EE32 (uncharacterized endoplasmic reticulum membrane protein YGL010W-like OS=Cucurbita moschata OX=3662 GN=LOC111431702 PE=4 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 4.0e-105
Identity = 190/198 (95.96%), Postives = 195/198 (98.48%), Query Frame = 0

Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF
Sbjct: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60

Query: 61  NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA 120
           NTGLV+NFG LFALVYA+FY+LFDKRAGSLAA MCFLCWVGASV AFKLGWTLTWKVVLA
Sbjct: 61  NTGLVLNFGFLFALVYAIFYILFDKRAGSLAAVMCFLCWVGASVFAFKLGWTLTWKVVLA 120

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFH+SVK
Sbjct: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHSSVK 180

Query: 181 AKMEAEKKAWEGKKEKKS 199
           AK+EAEKKAWEGKKEKKS
Sbjct: 181 AKIEAEKKAWEGKKEKKS 198

BLAST of CmaCh13G011410 vs. ExPASy TrEMBL
Match: A0A5D3BHY9 (Putative endoplasmic reticulum membrane protein-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001000 PE=4 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 7.3e-99
Identity = 179/198 (90.40%), Postives = 188/198 (94.95%), Query Frame = 0

Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNP NIFIHILFVWPIFFT LMLFYYTPSFYSFPKCPCGF
Sbjct: 1   MGRRGLFDLERHFAFYGAYHSNPINIFIHILFVWPIFFTGLMLFYYTPSFYSFPKCPCGF 60

Query: 61  NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA 120
           NTGLV+NFG LFAL+YAV Y+LFDKRAG LAAFMCFLCWVGASV AFKLGWTLTWKVVLA
Sbjct: 61  NTGLVLNFGFLFALLYAVVYILFDKRAGFLAAFMCFLCWVGASVFAFKLGWTLTWKVVLA 120

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFV+LEIL A FKYEPY+GFHA+VK
Sbjct: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVVLEILHALFKYEPYSGFHANVK 180

Query: 181 AKMEAEKKAWEGKKEKKS 199
           A++EAEKK W+ KKEKKS
Sbjct: 181 ARIEAEKKVWQDKKEKKS 198

BLAST of CmaCh13G011410 vs. ExPASy TrEMBL
Match: A0A1S3B9D3 (uncharacterized endoplasmic reticulum membrane protein YGL010W-like OS=Cucumis melo OX=3656 GN=LOC103487431 PE=4 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 7.3e-99
Identity = 179/198 (90.40%), Postives = 188/198 (94.95%), Query Frame = 0

Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNP NIFIHILFVWPIFFT LMLFYYTPSFYSFPKCPCGF
Sbjct: 1   MGRRGLFDLERHFAFYGAYHSNPINIFIHILFVWPIFFTGLMLFYYTPSFYSFPKCPCGF 60

Query: 61  NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA 120
           NTGLV+NFG LFAL+YAV Y+LFDKRAG LAAFMCFLCWVGASV AFKLGWTLTWKVVLA
Sbjct: 61  NTGLVLNFGFLFALLYAVVYILFDKRAGFLAAFMCFLCWVGASVFAFKLGWTLTWKVVLA 120

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFV+LEIL A FKYEPY+GFHA+VK
Sbjct: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVVLEILHALFKYEPYSGFHANVK 180

Query: 181 AKMEAEKKAWEGKKEKKS 199
           A++EAEKK W+ KKEKKS
Sbjct: 181 ARIEAEKKVWQDKKEKKS 198

BLAST of CmaCh13G011410 vs. NCBI nr
Match: XP_023000714.1 (uncharacterized endoplasmic reticulum membrane protein YGL010W-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 405.6 bits (1041), Expect = 2.5e-109
Identity = 199/199 (100.00%), Postives = 199/199 (100.00%), Query Frame = 0

Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF
Sbjct: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60

Query: 61  NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA 120
           NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA
Sbjct: 61  NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA 120

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK
Sbjct: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK 180

Query: 181 AKMEAEKKAWEGKKEKKSC 200
           AKMEAEKKAWEGKKEKKSC
Sbjct: 181 AKMEAEKKAWEGKKEKKSC 199

BLAST of CmaCh13G011410 vs. NCBI nr
Match: XP_023000712.1 (uncharacterized endoplasmic reticulum membrane protein YGL010W-like isoform X1 [Cucurbita maxima] >XP_023000713.1 uncharacterized endoplasmic reticulum membrane protein YGL010W-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 405.6 bits (1041), Expect = 2.5e-109
Identity = 199/199 (100.00%), Postives = 199/199 (100.00%), Query Frame = 0

Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF
Sbjct: 109 MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 168

Query: 61  NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA 120
           NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA
Sbjct: 169 NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA 228

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK
Sbjct: 229 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK 288

Query: 181 AKMEAEKKAWEGKKEKKSC 200
           AKMEAEKKAWEGKKEKKSC
Sbjct: 289 AKMEAEKKAWEGKKEKKSC 307

BLAST of CmaCh13G011410 vs. NCBI nr
Match: XP_023519470.1 (uncharacterized endoplasmic reticulum membrane protein YGL010W-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 391.0 bits (1003), Expect = 6.3e-105
Identity = 191/198 (96.46%), Postives = 195/198 (98.48%), Query Frame = 0

Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF
Sbjct: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60

Query: 61  NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA 120
           NTGLV+NFG LFALVYAVFY+LFDKRAGSLAAFMCFLCWVGASV AFKLGWTLTWKVVLA
Sbjct: 61  NTGLVLNFGFLFALVYAVFYILFDKRAGSLAAFMCFLCWVGASVFAFKLGWTLTWKVVLA 120

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQA FKYEPYNGFHASVK
Sbjct: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQALFKYEPYNGFHASVK 180

Query: 181 AKMEAEKKAWEGKKEKKS 199
           AK+EA+KKAWEGKKEKKS
Sbjct: 181 AKIEAKKKAWEGKKEKKS 198

BLAST of CmaCh13G011410 vs. NCBI nr
Match: XP_022924180.1 (uncharacterized endoplasmic reticulum membrane protein YGL010W-like [Cucurbita moschata] >KAG6584470.1 hypothetical protein SDJN03_20402, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 390.6 bits (1002), Expect = 8.2e-105
Identity = 190/198 (95.96%), Postives = 195/198 (98.48%), Query Frame = 0

Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF
Sbjct: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60

Query: 61  NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA 120
           NTGLV+NFG LFALVYA+FY+LFDKRAGSLAA MCFLCWVGASV AFKLGWTLTWKVVLA
Sbjct: 61  NTGLVLNFGFLFALVYAIFYILFDKRAGSLAAVMCFLCWVGASVFAFKLGWTLTWKVVLA 120

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFH+SVK
Sbjct: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHSSVK 180

Query: 181 AKMEAEKKAWEGKKEKKS 199
           AK+EAEKKAWEGKKEKKS
Sbjct: 181 AKIEAEKKAWEGKKEKKS 198

BLAST of CmaCh13G011410 vs. NCBI nr
Match: KAG7020057.1 (SPAC16E8.02 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 386.7 bits (992), Expect = 1.2e-103
Identity = 188/198 (94.95%), Postives = 193/198 (97.47%), Query Frame = 0

Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60
           MGRRG FDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF
Sbjct: 1   MGRRGSFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60

Query: 61  NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA 120
           NTGLV+NFG LFALVYA+FY+LFDKRAGSLAA MCFLCWVGASV AFKLGWTLTWKVV A
Sbjct: 61  NTGLVLNFGFLFALVYAIFYILFDKRAGSLAAVMCFLCWVGASVFAFKLGWTLTWKVVFA 120

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFH+SVK
Sbjct: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHSSVK 180

Query: 181 AKMEAEKKAWEGKKEKKS 199
           AK+EAEKKAWEGKKEKKS
Sbjct: 181 AKIEAEKKAWEGKKEKKS 198

BLAST of CmaCh13G011410 vs. TAIR 10
Match: AT1G18720.1 (Protein of unknown function (DUF962) )

HSP 1 Score: 234.6 bits (597), Expect = 7.0e-62
Identity = 121/200 (60.50%), Postives = 150/200 (75.00%), Query Frame = 0

Query: 5   GLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGFNTGL 64
           GLFDLE+HFAFYGAYHSNP NI IHI+FVWPIFF+ L+L + +     F     GF+  L
Sbjct: 6   GLFDLEKHFAFYGAYHSNPINILIHIIFVWPIFFSVLLLLHSSTPI--FDPSQLGFSQSL 65

Query: 65  VV------NFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVV 124
            +      N G +FAL+YA+FY+  DK++G +AA MCF CWVG+S LA +LG +L  KV 
Sbjct: 66  TLDGVLRFNVGFIFALIYALFYIGLDKKSGFVAALMCFSCWVGSSFLAVRLGSSLALKVG 125

Query: 125 LAAQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHAS 184
           LA+QL CWT QF+GHGVFEKRAPAL+DNL QAFLMAPFFVLLE+LQ+ F YEPY GF A 
Sbjct: 126 LASQLLCWTGQFVGHGVFEKRAPALLDNLLQAFLMAPFFVLLEVLQSVFGYEPYPGFQAR 185

Query: 185 VKAKMEAEKKAWEGKKEKKS 199
           V AK+E++ K +  KK+KK+
Sbjct: 186 VNAKVESDIKEYRAKKQKKN 203

BLAST of CmaCh13G011410 vs. TAIR 10
Match: AT1G74440.1 (Protein of unknown function (DUF962) )

HSP 1 Score: 214.9 bits (546), Expect = 5.8e-56
Identity = 111/199 (55.78%), Postives = 139/199 (69.85%), Query Frame = 0

Query: 3   RRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKC----PC 62
           R GL DLE+HFAFYGAYHSNP NI IH LFVWP  F  L+  Y TP      +       
Sbjct: 4   RIGLLDLEKHFAFYGAYHSNPINIIIHTLFVWPNVFATLLFLYSTPPILDHSQLGFLKSL 63

Query: 63  GFNTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVV 122
            F+  L ++ G    + YAVFY+  DK++G LAA +CF CW+G+S LA +LG +LT KV 
Sbjct: 64  TFDGVLRLDIGFTLTVTYAVFYICLDKKSGVLAALLCFSCWIGSSFLAARLGHSLTLKVG 123

Query: 123 LAAQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHAS 182
           +A+QL CWT QF+GHG+FEKRAPAL+DNL QAFLM PFFVLLE+LQ+ F YEPY GF A 
Sbjct: 124 VASQLLCWTGQFLGHGLFEKRAPALLDNLLQAFLMGPFFVLLEVLQSVFGYEPYPGFKAR 183

Query: 183 VKAKMEAEKKAWEGKKEKK 198
           V +K+E+  K W  KK+++
Sbjct: 184 VDSKIESGIKEWREKKKQQ 202

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O137372.7e-1836.462-hydroxy-palmitic acid dioxygenase mpo1 OS=Schizosaccharomyces pombe (strain 97... [more]
P253382.0e-1633.332-hydroxy-palmitic acid dioxygenase MPO1 OS=Saccharomyces cerevisiae (strain ATC... [more]
Match NameE-valueIdentityDescription
A0A6J1KEF11.2e-109100.00uncharacterized endoplasmic reticulum membrane protein YGL010W-like isoform X1 O... [more]
A0A6J1KKR61.2e-109100.00uncharacterized endoplasmic reticulum membrane protein YGL010W-like isoform X2 O... [more]
A0A6J1EE324.0e-10595.96uncharacterized endoplasmic reticulum membrane protein YGL010W-like OS=Cucurbita... [more]
A0A5D3BHY97.3e-9990.40Putative endoplasmic reticulum membrane protein-like protein OS=Cucumis melo var... [more]
A0A1S3B9D37.3e-9990.40uncharacterized endoplasmic reticulum membrane protein YGL010W-like OS=Cucumis m... [more]
Match NameE-valueIdentityDescription
XP_023000714.12.5e-109100.00uncharacterized endoplasmic reticulum membrane protein YGL010W-like isoform X2 [... [more]
XP_023000712.12.5e-109100.00uncharacterized endoplasmic reticulum membrane protein YGL010W-like isoform X1 [... [more]
XP_023519470.16.3e-10596.46uncharacterized endoplasmic reticulum membrane protein YGL010W-like [Cucurbita p... [more]
XP_022924180.18.2e-10595.96uncharacterized endoplasmic reticulum membrane protein YGL010W-like [Cucurbita m... [more]
KAG7020057.11.2e-10394.95SPAC16E8.02 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT1G18720.17.0e-6260.50Protein of unknown function (DUF962) [more]
AT1G74440.15.8e-5655.78Protein of unknown function (DUF962) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0093052-hydroxy-palmitic acid dioxygenase Mpo1-likePFAMPF06127DUF962coord: 8..164
e-value: 4.0E-29
score: 100.6
IPR0093052-hydroxy-palmitic acid dioxygenase Mpo1-likePANTHERPTHR28026DUF962 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G05310)coord: 1..196
NoneNo IPR availablePANTHERPTHR28026:SF8YGL010W-LIKE PROTEINcoord: 1..196

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh13G011410.1CmaCh13G011410.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046521 sphingoid catabolic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane