CmaCh13G010370 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh13G010370
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionAbhydro_lipase domain-containing protein
LocationCma_Chr13: 7916211 .. 7921332 (+)
RNA-Seq ExpressionCmaCh13G010370
SyntenyCmaCh13G010370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTTTTCCCTCTTCAGAGAGTTCCCACGCGCGCCCTTACCTGACACAGTCACCGGCCTAAGGCAAGAATAACCGACTTCCATTTACGTCAAGAATCGCCGTTGTTTTTTCTTTCGGAATATTCAAGTCCGATAATTTCTGTCTCTCTCTCTTTTTCTATTTCCTAATTTTGTTCTTCAGGTTTTCCGCTTCTTTCTGCGATTTGTTGCCTGAATTTGCGCCTCCATTTGGGTCTGGATATTGTTTATCTCTCTCTTTCTCCGTTTCCGTAATTTTTTTCGGATCGGGAAGAGATTGAAGAACCTTAATAAATGATTCAACGATTTGTTGACGATGTGCTTGCTGTTACCAAAGAGTGAGTCTTCTTCTTCCTTCCCCTTCCCAGTTGATCAAGTTTTTTTAACCTGAAACGGGGGATTTGATTTTATTGTTTTTTTGTGGGAATTTAGTTTTTGGCGGATGATGATGTGTGTTGTTCTCTCAGCTGATCACGCGCTGGAATTTGTTCTAAATTTATATAACAATCGAGCCAGAATTTTTTGATGATCTCACCTCATTGTTTTTGAAGCGAATTTAGGAAACTAATTAGTGCAAGTTAGTTGCGTAATAGATCTGGATTTGGATATGGATGCCTCTTTTGAATGCTCCAATCGCTTAATGAAATTGGTGCTGTTTAACTTGAGGAGCTGTTTCGGATTTTATTGAATCGTGCCATAGGCTTGTAGAATCTTTTTTCCTATTTGTTCCTCTTGTTCTTTCATCCATCTTAATTCCGTGCTTTCCGTCTGTATTTTTGTTTCAATGGAGAAATGGCAACTGTTTTTTATTGCTAATGCTTTCTTTTTTAACTTTCTCGTGGCCAATTTTAATCTGTAGGTGCATTTTTAACTTGCGTCTAAGAACTTCGTTCTGTTTGTCTACTTCGATTTATTTCCGTACAGGTGTTCATGTTTCTAACTAGTTGAGCTTATCTCCTTGAAGAAGTAACTTCTTTCCTTCTTGTATGTATATTTCTAGTGCAGGTCGGTGAAGACATTCACGTACGAGTCCTTGAATAATGTTGTGAGGCTGATCAATGGATTATCTGCACTTCTGCTGACTCTTTTACCAGGGAGGGGTTCGGTACTCGAAGGTGTTCATGGCTGGGAGCTCAGACCAACCTTTCGGGGGCCTCGTTTTCCTCGTTGGATGGAAAAGTATGTCCAATGACCTGAATTCTATGATGGTATAGGGTGTCCAGAATAATCCTTTAGAATCGATTTCAAGGCATGATTTTTATAAGTCCACGTTTTTACCCCACTAGGAAATTACCTGGTTATTATGATTTTCACAATTTTGACATATTTGGAAAACTTGGTTTGTTCTTTCAGTGATGAGAATAAGAATTATCATTATCATTTTTCATTTAATCATAACTCAAAAATATTTTAATCAATCTTTTATCACACTTCTTAATCTGTTTTTCTGTGGAGAAAATTTGAGATACTAATTTTAAAGTTTACATTCATGGTATAGTTGAGAGGGTTTTCTGCTATTCATTCATTCATACATGCATGGGAAAAAAATCGTCTCTAATCTAGTAGGCGTATTGCTCTAGTCAATATAGATGTAAGGCTAGAAATCAATTTTTTTTCTTCATATTTGCGTGAAATCAAGTTCGCTGAAGGCAATGATGGAGCATGAAGTTTGGAATAGTATCATTAATACATGAATCCACAATTGCACATTGTGTTAGTGGGAAAAAATGATGTCCTCTATAATTCACTGTTCCTGAAGATTAAAAAAAAGGAAAGAAAAAAACAAGACTGCGTTATTGGGTTCACCTCTTTGCAACATAACTGTTGGCCACTCCACCTTCCAGCATGATTTTTTGCTGGTATCCTGGTACCCTGAAAAGAGCCTTTCTACCCTCATGTCTGGTAGACTTTATGTTCTCCGTATAACTAGGTAGCTTTATGAGTTTACAAAGTCACTTAAGCGATTGTGACTTTAGTTATATGATAACGTAGGAAATAGAAGTAGAATTCCTCAAGGTTATAAAAGTGCCCTTTTTCCTGACATTAAGTGCACAAATTCCAAAACGGTGGCTTTCATTTTCTATAATTTAAGCTGGCTTCTTCGGATGGAATGGCTGTTTCCTTTTCTTTTATCCCTCTGGTAGTTTGAATTGCCATTGATATAGAGGACATGCATCACATTGTGTTTTTATGGTATTTTAGTTAATAAGTAGTTGACATATGTATATTTTTTCCTGTGGTACATAGTGGTGTGTCATCCTTCAACCAGTTCATTCACGAACTTTCTGTGGACTCTGATGATTCAAGCTTAGATTATTCCTCTGGGGAGGAAGATGGTATTGGAAATGAATATCCTGCAACACCATCATCTCAGAGTTCACGAGGCTCCAGGTCCAGGTCTGGTTTTCATGTCAAGAATGTTTGGCACTGGACAGAAAGGATCAGAAGTATCTTTCTTTGGATTCTTTTGCCTGCCAAGTTCCTTTTGGGGATTCCATTTCGAACTTTTCACTTCTTTTTTATTAAATGGTCTGGGTCCTCATCCAGCCCTGGAAGTCCCTGGCCCTCAATTAAGCGAGTGCACAGCCATAAGGAGCACGTTGTTCATAGCACCACTGATCGGAGACGTGGAGTTATTGAGGTTTCTCTCTAACATCTACTAGTAGCTTCTGGTTCTCCCATCCTTAACTTCACTTATTTCTTAGAAGTGTTGTTCTAAAACACCTAGATATGCTTTAATTGGCCTGACTTCTATTTATTAATTTCTCTTTGCTTTACATGTAAATTAGGACCTTCATCTTGCAAGTGAAATCTTTATCGAATCTGCTTTTGAGATGGTCCATAAGGCTGCACATTTTATCCTCTCACCATTAGACACCATTAGAACTTTGTTCAGATGGCTTTGGTCTTGGAGCAGTTGTGAGAGAGATCACGATGCAGATGTCATCGTGCCCACAGCTACCCTTGGAGATGATGATCCTGTGCCTAGTGAAAGGACCTACACTTTTCATCAGTCACTGAATACCGATGCTAGAACGTGCCAAGATGTTATTACAGAACTTGGGTAAGATCAGTGCTGGTAACTTCTCATTTTATTCTGGACTTTGGGTTCAGCATGAAGCTGTTGGTTTCTCATGTTTGTTTGGTCTTTCTTCCTATCTTAGGTATCCATATGAAGCTATTCGTGTTGTGACTAATGATGGATATGTTCTTCTTCTGGAAAGAATACCCAGGTACAATACTAGTTAATTTATTTTTGCCTACTTCAATTTTTTGTCCAAATGTTTTGTGGTTAATGCTTGCACTGTGATTTGCAGGCGTGATGCACGTAAAGTTCTTTATCTGCAGCATGGAATATTTGATTCTTCAATGGGGTGAGGGTTCCTCCCCCATTTCTCGCCAGTTTCTGTTTCACAGTTGTCATAGGCTGTTAGTTTAATCTTGTGTGTAGGATAGATAGGGAATATATTATGACTTCATGCTGCTTTGGCTATGGTCCATGACATCCTTGAATTTAAAGATCTGTATGATGGTAACACCAAAGTACATCTCAAGGATTATTTCACATATAACTTAGCACACCACATATACCCACACACGTACGTACAAACACATGCATGGAGCATAATGACAAGGTTGGATTCAATAACTGTTTAGGTTGGATGGGTAGGGAATTACACTGTAGCATACTTCTGTTATTGATCTACATCCGTTCTCTAACTGTACTAGTTCTTTTGTCTAACTAGTTGGGTGTCCAACGGGGTTGTTGGTTCGCCAGCCTTTGCAGCTTTTGACCAAGGTACTTGCGTTTGCTTCAGAAGTTCAAGCCTACTCAATGAGGCTGTGTTTATCCTCCTCTTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTTTTTTTTTTAGCAAGTTTTTAACAATTTAAAATACTAATAATTATTGCAGGGTATGATGTTTATCTTGGCAATTTCCGTGGCTTGGTCTCTAGAGAACATGTTGATAAGAATATATCTTCAAGACAGTAAGTCTGCGTTTTTCACTTTTTCATGTATCTACATTTAATTTAACAGCTAATCCCTAATCGAGGGCTAATATCATGGAGGCTTTCATCATGTGCCCCTGTGGATGTCATTCGCTGGCTGATTTTGTAAACTATCCAGGTACTGGAAATATTCCATAAATGAACATGCCATGGAGGATATTCCAGCGATGATTGAAAAAATTCATGAAGTTAAAACTTCTGAATTGAAGCTCAGCCAACCTGAACTTGCAGAAGAAACTGATAGTGACCAGCCATTCAAGCTTTGTGCACTGTGCCACAGCATGGGAGGTGCTGCTATGTTGATGTATGTTATTACACGGAGGATTGAAGAGAAACCACATAGATTATCTAGATTGGTCTTACTATCTCCTGCTGGTTTCCACGACGATGCCCCATTTATTTTCACTGTGATTGAGAATCTCTTGCTCCTGTTATCTCCACTTTTAGCTCCATTTGTGCCTGGCTTGTACATACCAACCAGATTTTTCCGTATGCTTCTCAACAAGTTGGCTCGCGATTTCCATCACTATCCTGCTGTTGGAGGTTTGGTTCAAACTGTGGTGAGTTACTTTTTAGGTGGGGACAGTTCAAATTGGGTTGGAGTTCTGGGAATTCCACACTATAACATGAATGATATGCCCGGAGTTTCATTTCGAGTAGGTCTCCATCTTGCTCAGATGAAGCATGCCAAGAAATTTAGAATGTTCGATTATGGAAATGCATCCCTGAACATGGAAGCTTATGGATCACCGGAGCCACTGGACTTGGGTGAATACTTCGGGCTTTTTGATATCCCTGTAGATTTAGTTGCTGGACGTAAGGACCAGGTAATAAGACCAACCATGGTGAAAAGGTACTACAAAATGATGAAAGATGCTGGTGTTGATGTATCATTCAACGAATTTGAATATGCTCATCTGGACTTCACTTTCTCGCACCGTGAGGAGCTCTTGGCTTATGTAATGTCTCGTCTACTTCTGGTCGGTGAGCCTGGATCGAAACCGAAACTGAAGCTGAAGCCCTCTCAGAAAGTACCAAAACTGAGACGAAATGACAAGCTTGACGGCTAG

mRNA sequence

GCTTTTCCCTCTTCAGAGAGTTCCCACGCGCGCCCTTACCTGACACAGTCACCGGCCTAAGGTTTTCCGCTTCTTTCTGCGATTTGTTGCCTGAATTTGCGCCTCCATTTGGGTCTGGATATTGTTTATCTCTCTCTTTCTCCGTTTCCGTAATTTTTTTCGGATCGGGAAGAGATTGAAGAACCTTAATAAATGATTCAACGATTTGTTGACGATGTGCTTGCTGTTACCAAAGAGTCGGTGAAGACATTCACGTACGAGTCCTTGAATAATGTTGTGAGGCTGATCAATGGATTATCTGCACTTCTGCTGACTCTTTTACCAGGGAGGGGTTCGGTACTCGAAGGTGTTCATGGCTGGGAGCTCAGACCAACCTTTCGGGGGCCTCGTTTTCCTCGTTGGATGGAAAATGGTGTGTCATCCTTCAACCAGTTCATTCACGAACTTTCTGTGGACTCTGATGATTCAAGCTTAGATTATTCCTCTGGGGAGGAAGATGGTATTGGAAATGAATATCCTGCAACACCATCATCTCAGAGTTCACGAGGCTCCAGGTCCAGGTCTGGTTTTCATGTCAAGAATGTTTGGCACTGGACAGAAAGGATCAGAAGTATCTTTCTTTGGATTCTTTTGCCTGCCAAGTTCCTTTTGGGGATTCCATTTCGAACTTTTCACTTCTTTTTTATTAAATGGTCTGGGTCCTCATCCAGCCCTGGAAGTCCCTGGCCCTCAATTAAGCGAGTGCACAGCCATAAGGAGCACGTTGTTCATAGCACCACTGATCGGAGACGTGGAGTTATTGAGGACCTTCATCTTGCAAGTGAAATCTTTATCGAATCTGCTTTTGAGATGGTCCATAAGGCTGCACATTTTATCCTCTCACCATTAGACACCATTAGAACTTTGTTCAGATGGCTTTGGTCTTGGAGCAGTTGTGAGAGAGATCACGATGCAGATGTCATCGTGCCCACAGCTACCCTTGGAGATGATGATCCTGTGCCTAGTGAAAGGACCTACACTTTTCATCAGTCACTGAATACCGATGCTAGAACGTGCCAAGATGTTATTACAGAACTTGGGTATCCATATGAAGCTATTCGTGTTGTGACTAATGATGGATATGTTCTTCTTCTGGAAAGAATACCCAGGCGTGATGCACGTAAAGTTCTTTATCTGCAGCATGGAATATTTGATTCTTCAATGGGTTGGGTGTCCAACGGGGTTGTTGGTTCGCCAGCCTTTGCAGCTTTTGACCAAGGGTATGATGTTTATCTTGGCAATTTCCGTGGCTTGGTCTCTAGAGAACATGTTGATAAGAATATATCTTCAAGACAGTACTGGAAATATTCCATAAATGAACATGCCATGGAGGATATTCCAGCGATGATTGAAAAAATTCATGAAGTTAAAACTTCTGAATTGAAGCTCAGCCAACCTGAACTTGCAGAAGAAACTGATAGTGACCAGCCATTCAAGCTTTGTGCACTGTGCCACAGCATGGGAGGTGCTGCTATGTTGATGTATGTTATTACACGGAGGATTGAAGAGAAACCACATAGATTATCTAGATTGGTCTTACTATCTCCTGCTGGTTTCCACGACGATGCCCCATTTATTTTCACTGTGATTGAGAATCTCTTGCTCCTGTTATCTCCACTTTTAGCTCCATTTGTGCCTGGCTTGTACATACCAACCAGATTTTTCCGTATGCTTCTCAACAAGTTGGCTCGCGATTTCCATCACTATCCTGCTGTTGGAGGTTTGGTTCAAACTGTGGTGAGTTACTTTTTAGGTGGGGACAGTTCAAATTGGGTTGGAGTTCTGGGAATTCCACACTATAACATGAATGATATGCCCGGAGTTTCATTTCGAGTAGGTCTCCATCTTGCTCAGATGAAGCATGCCAAGAAATTTAGAATGTTCGATTATGGAAATGCATCCCTGAACATGGAAGCTTATGGATCACCGGAGCCACTGGACTTGGGTGAATACTTCGGGCTTTTTGATATCCCTGTAGATTTAGTTGCTGGACGTAAGGACCAGGTAATAAGACCAACCATGGTGAAAAGGTACTACAAAATGATGAAAGATGCTGGTGTTGATGTATCATTCAACGAATTTGAATATGCTCATCTGGACTTCACTTTCTCGCACCGTGAGGAGCTCTTGGCTTATGTAATGTCTCGTCTACTTCTGGTCGGTGAGCCTGGATCGAAACCGAAACTGAAGCTGAAGCCCTCTCAGAAAGTACCAAAACTGAGACGAAATGACAAGCTTGACGGCTAG

Coding sequence (CDS)

ATGATTCAACGATTTGTTGACGATGTGCTTGCTGTTACCAAAGAGTCGGTGAAGACATTCACGTACGAGTCCTTGAATAATGTTGTGAGGCTGATCAATGGATTATCTGCACTTCTGCTGACTCTTTTACCAGGGAGGGGTTCGGTACTCGAAGGTGTTCATGGCTGGGAGCTCAGACCAACCTTTCGGGGGCCTCGTTTTCCTCGTTGGATGGAAAATGGTGTGTCATCCTTCAACCAGTTCATTCACGAACTTTCTGTGGACTCTGATGATTCAAGCTTAGATTATTCCTCTGGGGAGGAAGATGGTATTGGAAATGAATATCCTGCAACACCATCATCTCAGAGTTCACGAGGCTCCAGGTCCAGGTCTGGTTTTCATGTCAAGAATGTTTGGCACTGGACAGAAAGGATCAGAAGTATCTTTCTTTGGATTCTTTTGCCTGCCAAGTTCCTTTTGGGGATTCCATTTCGAACTTTTCACTTCTTTTTTATTAAATGGTCTGGGTCCTCATCCAGCCCTGGAAGTCCCTGGCCCTCAATTAAGCGAGTGCACAGCCATAAGGAGCACGTTGTTCATAGCACCACTGATCGGAGACGTGGAGTTATTGAGGACCTTCATCTTGCAAGTGAAATCTTTATCGAATCTGCTTTTGAGATGGTCCATAAGGCTGCACATTTTATCCTCTCACCATTAGACACCATTAGAACTTTGTTCAGATGGCTTTGGTCTTGGAGCAGTTGTGAGAGAGATCACGATGCAGATGTCATCGTGCCCACAGCTACCCTTGGAGATGATGATCCTGTGCCTAGTGAAAGGACCTACACTTTTCATCAGTCACTGAATACCGATGCTAGAACGTGCCAAGATGTTATTACAGAACTTGGGTATCCATATGAAGCTATTCGTGTTGTGACTAATGATGGATATGTTCTTCTTCTGGAAAGAATACCCAGGCGTGATGCACGTAAAGTTCTTTATCTGCAGCATGGAATATTTGATTCTTCAATGGGTTGGGTGTCCAACGGGGTTGTTGGTTCGCCAGCCTTTGCAGCTTTTGACCAAGGGTATGATGTTTATCTTGGCAATTTCCGTGGCTTGGTCTCTAGAGAACATGTTGATAAGAATATATCTTCAAGACAGTACTGGAAATATTCCATAAATGAACATGCCATGGAGGATATTCCAGCGATGATTGAAAAAATTCATGAAGTTAAAACTTCTGAATTGAAGCTCAGCCAACCTGAACTTGCAGAAGAAACTGATAGTGACCAGCCATTCAAGCTTTGTGCACTGTGCCACAGCATGGGAGGTGCTGCTATGTTGATGTATGTTATTACACGGAGGATTGAAGAGAAACCACATAGATTATCTAGATTGGTCTTACTATCTCCTGCTGGTTTCCACGACGATGCCCCATTTATTTTCACTGTGATTGAGAATCTCTTGCTCCTGTTATCTCCACTTTTAGCTCCATTTGTGCCTGGCTTGTACATACCAACCAGATTTTTCCGTATGCTTCTCAACAAGTTGGCTCGCGATTTCCATCACTATCCTGCTGTTGGAGGTTTGGTTCAAACTGTGGTGAGTTACTTTTTAGGTGGGGACAGTTCAAATTGGGTTGGAGTTCTGGGAATTCCACACTATAACATGAATGATATGCCCGGAGTTTCATTTCGAGTAGGTCTCCATCTTGCTCAGATGAAGCATGCCAAGAAATTTAGAATGTTCGATTATGGAAATGCATCCCTGAACATGGAAGCTTATGGATCACCGGAGCCACTGGACTTGGGTGAATACTTCGGGCTTTTTGATATCCCTGTAGATTTAGTTGCTGGACGTAAGGACCAGGTAATAAGACCAACCATGGTGAAAAGGTACTACAAAATGATGAAAGATGCTGGTGTTGATGTATCATTCAACGAATTTGAATATGCTCATCTGGACTTCACTTTCTCGCACCGTGAGGAGCTCTTGGCTTATGTAATGTCTCGTCTACTTCTGGTCGGTGAGCCTGGATCGAAACCGAAACTGAAGCTGAAGCCCTCTCAGAAAGTACCAAAACTGAGACGAAATGACAAGCTTGACGGCTAG

Protein sequence

MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRPTFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGSRSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPSIKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFRWLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVYLGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEETDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIENLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNWVGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLAYVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG
Homology
BLAST of CmaCh13G010370 vs. ExPASy Swiss-Prot
Match: Q5VYY2 (Lipase member M OS=Homo sapiens OX=9606 GN=LIPM PE=2 SV=2)

HSP 1 Score: 109.8 bits (273), Expect = 1.3e-22
Identity = 100/379 (26.39%), Postives = 169/379 (44.59%), Query Frame = 0

Query: 290 DVITELGYPYEAIRVVTNDGYVLLLERIPR-------RDARKVLYLQHGIFDSSMGWVSN 349
           ++I   GYP E   V T DGY+L + RIPR         +R V+ LQHG+   +  W+SN
Sbjct: 51  EIIQHQGYPCEEYEVATEDGYILSVNRIPRGLVQPKKTGSRPVVLLQHGLVGGASNWISN 110

Query: 350 GVVGSPAFAAFDQGYDVYLGNFRGLV-SREHVDKNISSRQYWKYSINEHAMEDIPAMIEK 409
               S  F   D G+DV++GN RG   SR+H   +I   ++W +S +E A  D+PA+I  
Sbjct: 111 LPNNSLGFILADAGFDVWMGNSRGNAWSRKHKTLSIDQDEFWAFSYDEMARFDLPAVINF 170

Query: 410 IHEVKTSELKLSQPELAEETDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLV 469
           I + KT + K+     ++ T             +MG      ++    + E   ++    
Sbjct: 171 ILQ-KTGQEKIYYVGYSQGT-------------TMG------FIAFSTMPELAQKIKMYF 230

Query: 470 LLSPAGF--HDDAPFIFTVIENLLLLLSPLLAPFVPGLY------IPTRFFRMLLNKLAR 529
            L+P     H  +P          LLL  ++   + GL+        TRF R L+  L  
Sbjct: 231 ALAPIATVKHAKSP------GTKFLLLPDMM---IKGLFGKKEFLYQTRFLRQLVIYLCG 290

Query: 530 DFHHYPAVGGLVQTVVSYFLGGDSSNWVGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKK 589
                  +   + + +   LGG ++N + +     Y  + + G S +  LH +Q  ++ +
Sbjct: 291 Q-----VILDQICSNIMLLLGGFNTNNMNMSRASVYAAHTLAGTSVQNILHWSQAVNSGE 350

Query: 590 FRMFDYGNASLNMEAYGSPEPLDLGEYFGLFD--IPVDLVAGRKDQVIRPTMVKRYYKMM 649
            R FD+G+ + N+E    P P+     + + D  +P  +  G +D +  P  VK    + 
Sbjct: 351 LRAFDWGSETKNLEKCNQPTPV----RYRVRDMTVPTAMWTGGQDWLSNPEDVKML--LS 389

Query: 650 KDAGVDVSFNEFEYAHLDF 651
           +   +    N  E+AH+DF
Sbjct: 411 EVTNLIYHKNIPEWAHVDF 389

BLAST of CmaCh13G010370 vs. ExPASy Swiss-Prot
Match: Q8K2A6 (Lipase member M OS=Mus musculus OX=10090 GN=Lipm PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 1.7e-22
Identity = 101/374 (27.01%), Postives = 165/374 (44.12%), Query Frame = 0

Query: 290 DVITELGYPYEAIRVVTNDGYVLLLERIPR-------RDARKVLYLQHGIFDSSMGWVSN 349
           ++I   GYP E   V T DGY+L + RIPR         +R V+ LQHG+   +  W+SN
Sbjct: 51  EIIKHKGYPSEEYEVATEDGYILSVNRIPRGQTRLKKEGSRPVVLLQHGLLGDASNWISN 110

Query: 350 GVVGSPAFAAFDQGYDVYLGNFRGLV-SREHVDKNISSRQYWKYSINEHAMEDIPAMIEK 409
               S  F   D G+DV++GN RG   SR+H   +I   ++W +S +E A  D+PA+I  
Sbjct: 111 LPNNSLGFILADAGFDVWMGNSRGNTWSRKHKTLSIDQDEFWAFSYDEMARFDLPAVINF 170

Query: 410 IHEVKTSELKLSQPELAEETDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLV 469
           I + KT + K+     ++ T             +MG      ++    + E  H++    
Sbjct: 171 ILQ-KTGQKKVYYVGYSQGT-------------TMG------FIAFSTMPELAHKIKMYF 230

Query: 470 LLSPAGFHDDAPFIFTVIENLLLLLSPLLAPFVPG---LYIPTRFFRMLLNKLARDFHHY 529
            L+P      A    T      LLL  ++   + G       TRFFR L   L       
Sbjct: 231 ALAPIATVKYARSPGT----KFLLLPDMMIKVLFGRQEFLYQTRFFRQLFIYLCGQM--- 290

Query: 530 PAVGGLVQTVVSYFLGGDSSNWVGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFD 589
             +   + + +   LGG ++N + +     Y  +   G S +  LH +Q  ++ + R FD
Sbjct: 291 --ILDQICSNIILLLGGFNTNNMNMSRANVYVAHTPAGTSVQNILHWSQAVNSGELRAFD 350

Query: 590 YGNASLNMEAYGSPEPLDLGEYFGLFD--IPVDLVAGRKDQVIRPTMVKRYYKMMKDAGV 649
           +G+ + N E    P P+     + + D  +P  +  G +D +  P  VK    + +   +
Sbjct: 351 WGSETKNQEKCNQPTPI----RYKVRDMMVPTAMWTGGQDWLSNPDDVKTL--LSEVTNL 389

Query: 650 DVSFNEFEYAHLDF 651
               N  E+AH+DF
Sbjct: 411 IYHKNIPEWAHVDF 389

BLAST of CmaCh13G010370 vs. ExPASy Swiss-Prot
Match: Q3U4B4 (Lipase member N OS=Mus musculus OX=10090 GN=Lipn PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.9e-22
Identity = 105/392 (26.79%), Postives = 166/392 (42.35%), Query Frame = 0

Query: 277 FHQSLNTDA-RTCQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDA-------RKVLYL 336
           F   +N +      ++I   GYP E   V T DGY+L + RIP   A       R V+Y+
Sbjct: 26  FENKVNPEVWMNASEIIMYNGYPSEEYDVTTADGYILAINRIPHGRAQTGQTGPRPVVYM 85

Query: 337 QHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVYLGNFRGLV-SREHVDKNISSRQYWKYSI 396
           QH +F  +  W+ N   GS  F   D GYDV++GN RG   SR H   + +  ++W +S 
Sbjct: 86  QHALFADNAYWLENFANGSLGFILADAGYDVWMGNSRGNTWSRRHKTLSANEEKFWAFSF 145

Query: 397 NEHAMEDIPAMIEKIHEVKTSELKLSQPELAEETDSDQPFKLCALCHSMGGAAMLMYVIT 456
           NE A  D+P +I+ I      E                  KL  + HS+G    + +V  
Sbjct: 146 NEMAKYDLPGIIDFIVNKTGQE------------------KLYFIGHSLG--TTIGFVAF 205

Query: 457 RRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIENLLLLLSPLLAPFVPGLYIPTRFFRML 516
             + E   R+     L P         +FT   NL LL   ++      L   T+   +L
Sbjct: 206 STMPELAQRIKMNFALGPVISFKYPTSVFT---NLFLLPKSIIK-----LVFGTKGV-LL 265

Query: 517 LNKLAR----DFHHYPAVGGLVQTVVSYFLGGDSSNWVGVLGIPHYNMNDMPGVSFRVGL 576
            +K AR     F +   +  L    +S + G +  N + +  +  Y  +   G S +  L
Sbjct: 266 EDKNARMSFITFCNQKLLQPLCSEFMSLWAGFNKKN-MNMSRLDVYMAHAPTGSSIQNML 325

Query: 577 HLAQMKHAKKFRMFDYGNASLNMEAYGSPEP--LDLGEYFGLFDIPVDLVAGRKDQVIRP 636
           H+ Q+  + +FR +D+G+ + NM  Y    P   DL        +P  + AG  D ++ P
Sbjct: 326 HIKQLYRSDEFRAYDWGSEAENMNHYNQSYPPLYDL----TAMKVPTAIWAGGHDVLVTP 380

Query: 637 TMVKRYYKMMKDAGVDVSFNEF-EYAHLDFTF 653
             V R    + +      F +F ++ H DF +
Sbjct: 386 QDVARILPQITNLRY---FKQFPDWNHFDFVW 380

BLAST of CmaCh13G010370 vs. ExPASy Swiss-Prot
Match: O46108 (Lipase 3 OS=Drosophila melanogaster OX=7227 GN=Lip3 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 4.9e-22
Identity = 110/398 (27.64%), Postives = 157/398 (39.45%), Query Frame = 0

Query: 288 CQDVITELGYPYEAIRVVTNDGYVLLLERIP------RRDARKVLYLQHGIFDSSMGWVS 347
           C + I + GYP E   VVT+D Y+L + RIP          R V +L HG+  SS  WV 
Sbjct: 28  CGERIEDDGYPMERHEVVTSDNYILTMHRIPYSPKTGESSNRPVAFLMHGMLSSSSDWVL 87

Query: 348 NGVVGSPAFAAFDQGYDVYLGNFRG-LVSREHVDKNISSRQYWKYSINEHAMEDIPAMIE 407
            G   S A+   D GYDV++GN RG   S+ H       + +W +S NE  M D+PAMI+
Sbjct: 88  MGPERSLAYMLADAGYDVWMGNARGNTYSKAHKYWPTYWQIFWNFSWNEIGMYDVPAMID 147

Query: 408 KIHEVKTSELKLSQPELAEETDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRL 467
            +   KT + ++                   + HS G    L+ V  R   E   ++   
Sbjct: 148 YV-LAKTGQQQVQY-----------------VGHSQGTTVYLVMVSER--PEYNDKIKSA 207

Query: 468 VLLSPAGFHDDAPFIFTVIENLLLLLSPLLAPFVPGLYIPTRFFR-------MLLNKLAR 527
            LL PA +  +             + SPL   F P L  P            M  NK  +
Sbjct: 208 HLLGPAAYMGN-------------MKSPLTRAFAPILGQPNAIVEVCGSMEFMPSNKFKQ 267

Query: 528 D--FHHYPAVGGLVQTVVS--YFLGGDSSNWVGVLGIPHYNMNDMPGVSFRVGLHLAQMK 587
           D       A         +  + +GG  +  +    + H       G S    LH  Q  
Sbjct: 268 DLGIEMCQATSPYADMCANEIFLIGGYDTEQLDYELLEHIKATSPAGASVNQNLHFCQEY 327

Query: 588 HAKKFRMFDYGNASLNMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYK 647
           ++ KFR FDY  A  N   YGS  P D          PV L  G  D +   + V++   
Sbjct: 328 NSGKFRKFDY-TALRNPYEYGSYFPPDY--KLKNAKAPVLLYYGANDWMCDVSDVRKLRD 387

Query: 648 MMKDAGVDVSFNEFEYAHLDFTFSHREELLAYVMSRLL 668
            + +  +D      ++AHLDF +    E   YV   +L
Sbjct: 388 ELPNMALDYLVPFEKWAHLDFIWG--TEARKYVYDEVL 387

BLAST of CmaCh13G010370 vs. ExPASy Swiss-Prot
Match: P80035 (Gastric triacylglycerol lipase OS=Canis lupus familiaris OX=9615 GN=LIPF PE=1 SV=2)

HSP 1 Score: 107.8 bits (268), Expect = 4.9e-22
Identity = 99/342 (28.95%), Postives = 156/342 (45.61%), Query Frame = 0

Query: 291 VITELGYPYEAIRVVTNDGYVLLLERIP--RRDA-----RKVLYLQHGIFDSSMGWVSNG 350
           +IT  GYP E   VVT DGY+L ++RIP  R+++     R V +LQHG+  S+  W+SN 
Sbjct: 38  MITYWGYPAEEYEVVTEDGYILGIDRIPYGRKNSENIGRRPVAFLQHGLLASATNWISNL 97

Query: 351 VVGSPAFAAFDQGYDVYLGNFRGLV-SREHVDKNISSRQYWKYSINEHAMEDIPAMIEKI 410
              S AF   D GYDV+LGN RG   +R ++  +  S ++W +S +E A  D+PA I+ I
Sbjct: 98  PNNSLAFILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKYDLPATIDFI 157

Query: 411 HEVKTSELKLSQPELAEETDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVL 470
                           ++T  D   KL  + HS G    + ++      +   R+     
Sbjct: 158 ---------------LKKTGQD---KLHYVGHSQG--TTIGFIAFSTNPKLAKRIKTFYA 217

Query: 471 LSPAGFHDDAPFIFTVIENLLLLLSPLL-APFVPGLYIPTRFFRMLLNKLARDFHHYPAV 530
           L+P        +  T++  L+L+ S L    F   ++ P  FF      LA +      V
Sbjct: 218 LAPVA---TVKYTETLLNKLMLVPSFLFKLIFGNKIFYPHHFFDQF---LATEVCSRETV 277

Query: 531 GGLVQTVVSYFLGGDSSNWVGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGN 590
             L    +    G D+ N + +  +  Y  ++  G S +  LH +Q   + KF+ FD+G+
Sbjct: 278 DLLCSNALFIICGFDTMN-LNMSRLDVYLSHNPAGTSVQNVLHWSQAVKSGKFQAFDWGS 337

Query: 591 ASLNMEAYGSPEPLDLGEYFGLFD--IPVDLVAGRKDQVIRP 622
              NM  Y    P     Y+ L D  +P+ +  G  D +  P
Sbjct: 338 PVQNMMHYHQSMP----PYYNLTDMHVPIAVWNGGNDLLADP 348

BLAST of CmaCh13G010370 vs. ExPASy TrEMBL
Match: A0A6J1KNX8 (uncharacterized protein LOC111495192 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495192 PE=4 SV=1)

HSP 1 Score: 1417.5 bits (3668), Expect = 0.0e+00
Identity = 697/697 (100.00%), Postives = 697/697 (100.00%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60
           MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP
Sbjct: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120

Query: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180
           RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS
Sbjct: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180

Query: 181 IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR 240
           IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR
Sbjct: 181 IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR 240

Query: 241 WLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE 300
           WLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE
Sbjct: 241 WLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE 300

Query: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY 360
           AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY
Sbjct: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY 360

Query: 361 LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE 420
           LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE
Sbjct: 361 LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE 420

Query: 421 TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480
           TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE
Sbjct: 421 TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480

Query: 481 NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW 540
           NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW
Sbjct: 481 NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW 540

Query: 541 VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY 600
           VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY
Sbjct: 541 VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY 600

Query: 601 FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA 660
           FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA
Sbjct: 601 FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA 660

Query: 661 YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG 698
           YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG
Sbjct: 661 YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG 697

BLAST of CmaCh13G010370 vs. ExPASy TrEMBL
Match: A0A6J1EB22 (uncharacterized protein LOC111431539 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431539 PE=4 SV=1)

HSP 1 Score: 1412.5 bits (3655), Expect = 0.0e+00
Identity = 695/697 (99.71%), Postives = 695/697 (99.71%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60
           MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP
Sbjct: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120

Query: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180
           RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS
Sbjct: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180

Query: 181 IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR 240
           IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR
Sbjct: 181 IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR 240

Query: 241 WLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE 300
           WLWSWSSCERDHDA VIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE
Sbjct: 241 WLWSWSSCERDHDAGVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE 300

Query: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY 360
           AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY
Sbjct: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY 360

Query: 361 LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE 420
           LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE
Sbjct: 361 LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE 420

Query: 421 TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480
           TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE
Sbjct: 421 TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480

Query: 481 NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW 540
           NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW
Sbjct: 481 NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW 540

Query: 541 VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY 600
           VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNAS NMEAYGSPEPLDLGEY
Sbjct: 541 VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASQNMEAYGSPEPLDLGEY 600

Query: 601 FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA 660
           FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA
Sbjct: 601 FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA 660

Query: 661 YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG 698
           YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG
Sbjct: 661 YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG 697

BLAST of CmaCh13G010370 vs. ExPASy TrEMBL
Match: A0A1S3C3R6 (uncharacterized protein LOC103496491 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496491 PE=4 SV=1)

HSP 1 Score: 1291.2 bits (3340), Expect = 0.0e+00
Identity = 637/701 (90.87%), Postives = 662/701 (94.44%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60
           MIQR VDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPG+GSVLEGV+GWELRP
Sbjct: 1   MIQRVVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGKGSVLEGVNGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDG GNEYPATPSSQ SRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATPSSQGSRGS 120

Query: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180
           RSR+GFH KNVWHWTERIRSI  WILLPA+FL GIPFR FHFFFIKWSGS+++PGSPWPS
Sbjct: 121 RSRAGFHAKNVWHWTERIRSIIFWILLPARFLFGIPFRIFHFFFIKWSGSTTTPGSPWPS 180

Query: 181 IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR 240
           I+R+HSHK+HVVH TTDRRRGVIEDLHLASEIFIES FE+VHKAAHFILSPLD +RT FR
Sbjct: 181 IRRIHSHKDHVVH-TTDRRRGVIEDLHLASEIFIESVFEIVHKAAHFILSPLDALRTSFR 240

Query: 241 WLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE 300
           WL+   SCE  HDADV VPTATLGDDD  PSER+YTFHQSLNTDARTCQDVITELGYPYE
Sbjct: 241 WLFPRDSCESYHDADVTVPTATLGDDDSAPSERSYTFHQSLNTDARTCQDVITELGYPYE 300

Query: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY 360
           AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDV+
Sbjct: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVF 360

Query: 361 LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE 420
           LGNFRGLVSREH+DKNISSRQYWKYSINEHAMEDIPAMIEKI+EVKTSELKLSQPE+ +E
Sbjct: 361 LGNFRGLVSREHIDKNISSRQYWKYSINEHAMEDIPAMIEKIYEVKTSELKLSQPEIVKE 420

Query: 421 TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480
           TD+DQPFKLCALCHSMGGA MLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE
Sbjct: 421 TDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480

Query: 481 NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW 540
           NLLLLL+P+LAPFVPGLYIPTRF RMLLNKLARDF HYPAVGGLVQTVVSYFLGGDSSNW
Sbjct: 481 NLLLLLAPILAPFVPGLYIPTRFCRMLLNKLARDFQHYPAVGGLVQTVVSYFLGGDSSNW 540

Query: 541 VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY 600
           VGVLG PHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGN SLNMEAYGSPEPLDLGEY
Sbjct: 541 VGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNPSLNMEAYGSPEPLDLGEY 600

Query: 601 FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA 660
           FGL DIPVDLVAGRKDQVIRPTMVKRYY MMKDAGVDVSFNEFEYAHLDFTFSHREELL+
Sbjct: 601 FGLIDIPVDLVAGRKDQVIRPTMVKRYYSMMKDAGVDVSFNEFEYAHLDFTFSHREELLS 660

Query: 661 YVMSRLLLVGEPGSKPKLKLKPSQ----KVPKLRRNDKLDG 698
           YVMSRLLLV EPGS  K K KPSQ    KVPKL+R + LDG
Sbjct: 661 YVMSRLLLVDEPGS--KRKPKPSQQKVSKVPKLKRKETLDG 698

BLAST of CmaCh13G010370 vs. ExPASy TrEMBL
Match: A0A0A0LQR4 (Abhydro_lipase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042200 PE=4 SV=1)

HSP 1 Score: 1289.6 bits (3336), Expect = 0.0e+00
Identity = 636/697 (91.25%), Postives = 660/697 (94.69%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60
           MIQR VDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPG+GSVLEGV+GWELRP
Sbjct: 1   MIQRVVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGKGSVLEGVNGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDG GNEYPATPSSQ SRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATPSSQGSRGS 120

Query: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180
           RSR GFH K+VWHWTERIRSI  WILLPAKFLLGIPFR FHFFFIKWSGS+++PGSPWPS
Sbjct: 121 RSRVGFHAKSVWHWTERIRSIVFWILLPAKFLLGIPFRIFHFFFIKWSGSTTTPGSPWPS 180

Query: 181 IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR 240
           I+RVHSHK+HVVH TTDRRRGVIEDLHLASEIFIES FE+VHKAAHFILSPLD +RT FR
Sbjct: 181 IRRVHSHKDHVVH-TTDRRRGVIEDLHLASEIFIESVFEIVHKAAHFILSPLDALRTSFR 240

Query: 241 WLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE 300
           W +   SCER HDADV VPTATLGDDD  PSER+YTFHQSLNTDARTCQDVITELGYPYE
Sbjct: 241 WFFPRDSCERYHDADVTVPTATLGDDDSAPSERSYTFHQSLNTDARTCQDVITELGYPYE 300

Query: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY 360
           AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDV+
Sbjct: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVF 360

Query: 361 LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE 420
           LGNFRGLVSREH+DK ISSRQYWKYSINEHAMEDIPAMIEKI+EVK SELKLSQPE+AE 
Sbjct: 361 LGNFRGLVSREHIDKKISSRQYWKYSINEHAMEDIPAMIEKIYEVKNSELKLSQPEIAEV 420

Query: 421 TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480
           TD+DQPFKLCALCHSMGGA MLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE
Sbjct: 421 TDNDQPFKLCALCHSMGGAGMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480

Query: 481 NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW 540
           NLLLLL+P+LAPFVPGLYIPTRF RMLLNKLARDF HYPAVGGLVQTVVSYFLGGDSSNW
Sbjct: 481 NLLLLLAPILAPFVPGLYIPTRFCRMLLNKLARDFQHYPAVGGLVQTVVSYFLGGDSSNW 540

Query: 541 VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY 600
           VGVLG PHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY
Sbjct: 541 VGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY 600

Query: 601 FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA 660
           FGL DIPVDLVAGRKDQVIRPTMVKRYY+MMKDA VDVSFNEFEYAHLDFTFSHREELL+
Sbjct: 601 FGLIDIPVDLVAGRKDQVIRPTMVKRYYRMMKDAAVDVSFNEFEYAHLDFTFSHREELLS 660

Query: 661 YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG 698
           YVMSRLLLV EPGSK K K+    KVPKL+R +KLDG
Sbjct: 661 YVMSRLLLVDEPGSKLKPKV---SKVPKLKRKEKLDG 693

BLAST of CmaCh13G010370 vs. ExPASy TrEMBL
Match: A0A6J1KL85 (lipase member N isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495192 PE=4 SV=1)

HSP 1 Score: 1288.1 bits (3332), Expect = 0.0e+00
Identity = 647/697 (92.83%), Postives = 647/697 (92.83%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60
           MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP
Sbjct: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120

Query: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180
           R                                                  SSPGSPWPS
Sbjct: 121 R--------------------------------------------------SSPGSPWPS 180

Query: 181 IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR 240
           IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR
Sbjct: 181 IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR 240

Query: 241 WLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE 300
           WLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE
Sbjct: 241 WLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE 300

Query: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY 360
           AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY
Sbjct: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY 360

Query: 361 LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE 420
           LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE
Sbjct: 361 LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE 420

Query: 421 TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480
           TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE
Sbjct: 421 TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480

Query: 481 NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW 540
           NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW
Sbjct: 481 NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW 540

Query: 541 VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY 600
           VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY
Sbjct: 541 VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY 600

Query: 601 FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA 660
           FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA
Sbjct: 601 FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA 647

Query: 661 YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG 698
           YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG
Sbjct: 661 YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG 647

BLAST of CmaCh13G010370 vs. NCBI nr
Match: XP_023000888.1 (uncharacterized protein LOC111495192 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1417.5 bits (3668), Expect = 0.0e+00
Identity = 697/697 (100.00%), Postives = 697/697 (100.00%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60
           MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP
Sbjct: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120

Query: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180
           RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS
Sbjct: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180

Query: 181 IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR 240
           IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR
Sbjct: 181 IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR 240

Query: 241 WLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE 300
           WLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE
Sbjct: 241 WLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE 300

Query: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY 360
           AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY
Sbjct: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY 360

Query: 361 LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE 420
           LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE
Sbjct: 361 LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE 420

Query: 421 TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480
           TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE
Sbjct: 421 TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480

Query: 481 NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW 540
           NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW
Sbjct: 481 NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW 540

Query: 541 VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY 600
           VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY
Sbjct: 541 VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY 600

Query: 601 FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA 660
           FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA
Sbjct: 601 FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA 660

Query: 661 YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG 698
           YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG
Sbjct: 661 YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG 697

BLAST of CmaCh13G010370 vs. NCBI nr
Match: KAG6584371.1 (Lipase member N, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019955.1 Lipase member N [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1414.8 bits (3661), Expect = 0.0e+00
Identity = 696/697 (99.86%), Postives = 696/697 (99.86%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60
           MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP
Sbjct: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120

Query: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180
           RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS
Sbjct: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180

Query: 181 IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR 240
           IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR
Sbjct: 181 IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR 240

Query: 241 WLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE 300
           WLWSWSSCERDHDA VIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE
Sbjct: 241 WLWSWSSCERDHDAGVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE 300

Query: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY 360
           AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY
Sbjct: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY 360

Query: 361 LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE 420
           LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE
Sbjct: 361 LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE 420

Query: 421 TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480
           TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE
Sbjct: 421 TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480

Query: 481 NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW 540
           NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW
Sbjct: 481 NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW 540

Query: 541 VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY 600
           VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY
Sbjct: 541 VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY 600

Query: 601 FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA 660
           FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA
Sbjct: 601 FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA 660

Query: 661 YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG 698
           YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG
Sbjct: 661 YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG 697

BLAST of CmaCh13G010370 vs. NCBI nr
Match: XP_022923983.1 (uncharacterized protein LOC111431539 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1412.5 bits (3655), Expect = 0.0e+00
Identity = 695/697 (99.71%), Postives = 695/697 (99.71%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60
           MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP
Sbjct: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120

Query: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180
           RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS
Sbjct: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180

Query: 181 IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR 240
           IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR
Sbjct: 181 IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR 240

Query: 241 WLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE 300
           WLWSWSSCERDHDA VIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE
Sbjct: 241 WLWSWSSCERDHDAGVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE 300

Query: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY 360
           AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY
Sbjct: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY 360

Query: 361 LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE 420
           LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE
Sbjct: 361 LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE 420

Query: 421 TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480
           TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE
Sbjct: 421 TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480

Query: 481 NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW 540
           NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW
Sbjct: 481 NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW 540

Query: 541 VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY 600
           VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNAS NMEAYGSPEPLDLGEY
Sbjct: 541 VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASQNMEAYGSPEPLDLGEY 600

Query: 601 FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA 660
           FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA
Sbjct: 601 FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA 660

Query: 661 YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG 698
           YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG
Sbjct: 661 YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG 697

BLAST of CmaCh13G010370 vs. NCBI nr
Match: XP_023519485.1 (uncharacterized protein LOC111782880 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1408.7 bits (3645), Expect = 0.0e+00
Identity = 693/697 (99.43%), Postives = 695/697 (99.71%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60
           MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP
Sbjct: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120

Query: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180
           RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFR FHFFFIKWSGSSSSPGSPWPS
Sbjct: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRIFHFFFIKWSGSSSSPGSPWPS 180

Query: 181 IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR 240
           IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPL+TIRTLFR
Sbjct: 181 IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLETIRTLFR 240

Query: 241 WLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE 300
           WLWSWSSCERDH+ADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE
Sbjct: 241 WLWSWSSCERDHNADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE 300

Query: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY 360
           AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY
Sbjct: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY 360

Query: 361 LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE 420
           LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE
Sbjct: 361 LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE 420

Query: 421 TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480
           TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE
Sbjct: 421 TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480

Query: 481 NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW 540
           NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW
Sbjct: 481 NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW 540

Query: 541 VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY 600
           VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY
Sbjct: 541 VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY 600

Query: 601 FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA 660
           FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA
Sbjct: 601 FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA 660

Query: 661 YVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDKLDG 698
           YVMSRLLLVGE GSKPKLKLKPSQKVPKLRRNDKLDG
Sbjct: 661 YVMSRLLLVGESGSKPKLKLKPSQKVPKLRRNDKLDG 697

BLAST of CmaCh13G010370 vs. NCBI nr
Match: XP_038894357.1 (uncharacterized protein LOC120082972 isoform X1 [Benincasa hispida])

HSP 1 Score: 1315.4 bits (3403), Expect = 0.0e+00
Identity = 648/698 (92.84%), Postives = 668/698 (95.70%), Query Frame = 0

Query: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRP 60
           MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPG+GSVLEGVHGWELRP
Sbjct: 1   MIQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGKGSVLEGVHGWELRP 60

Query: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGS 120
           TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDG GNEYPATPSSQ SRGS
Sbjct: 61  TFRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGFGNEYPATPSSQGSRGS 120

Query: 121 RSRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSPGSPWPS 180
            SR+GFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFR FHFFFIKWSGS S+PGSPWP 
Sbjct: 121 MSRAGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRIFHFFFIKWSGSPSTPGSPWPL 180

Query: 181 IKRVHSHKEHVVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFR 240
           I+RVHSHK+HVVH TTDRRRGVIEDLHLASEIFIES FEMVHKAAHFILSPLD  RTLFR
Sbjct: 181 IRRVHSHKDHVVH-TTDRRRGVIEDLHLASEIFIESVFEMVHKAAHFILSPLDAFRTLFR 240

Query: 241 WLWSWSSCERDHDADVIVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDVITELGYPYE 300
           WL+  +SCER HDA+V VPTATLGDDDPVPSER YTFHQSLNTDARTCQDVITELGYPYE
Sbjct: 241 WLFPRNSCERYHDANVTVPTATLGDDDPVPSERNYTFHQSLNTDARTCQDVITELGYPYE 300

Query: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVY 360
           AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDV+
Sbjct: 301 AIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGYDVF 360

Query: 361 LGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPELAEE 420
           LGNFRGLVSREH+DKNISSRQYWKYSINEHAMEDIPAMI KIHEVKTSELKLSQPELAEE
Sbjct: 361 LGNFRGLVSREHIDKNISSRQYWKYSINEHAMEDIPAMIGKIHEVKTSELKLSQPELAEE 420

Query: 421 TDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480
            D+DQPFKLCALCHSMGGA +LMYVITRRI+EKPHRLSRLVLLSPAGFHDDAPFIFTVIE
Sbjct: 421 PDNDQPFKLCALCHSMGGAGILMYVITRRIQEKPHRLSRLVLLSPAGFHDDAPFIFTVIE 480

Query: 481 NLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW 540
           NLLLLL+P+LAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW
Sbjct: 481 NLLLLLAPILAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDSSNW 540

Query: 541 VGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDLGEY 600
           VGVLG PHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYG+ASLNMEAYGSPEPLDLGEY
Sbjct: 541 VGVLGTPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGSASLNMEAYGSPEPLDLGEY 600

Query: 601 FGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREELLA 660
           FGL DIPVDLVAGRKDQVIRPTMV+RYYKMMK+AGVDVSFNEFEYAHLDFTFSHREELL+
Sbjct: 601 FGLIDIPVDLVAGRKDQVIRPTMVRRYYKMMKNAGVDVSFNEFEYAHLDFTFSHREELLS 660

Query: 661 YVMSRLLLVGEPGSKPKLKLKPS-QKVPKLRRNDKLDG 698
           YVMSRLLLV EPGSK KLK      KVPKL+R DK DG
Sbjct: 661 YVMSRLLLVDEPGSKRKLKTSQKVSKVPKLKRKDKQDG 697

BLAST of CmaCh13G010370 vs. TAIR 10
Match: AT1G73920.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 918.7 bits (2373), Expect = 2.8e-267
Identity = 464/706 (65.72%), Postives = 556/706 (78.75%), Query Frame = 0

Query: 2   IQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRPT 61
           +QR VD+ LAVTKESVKT TYESLNN+ R ING+SALLLTLLPG+ ++LEG+HGWELRPT
Sbjct: 1   MQRIVDNALAVTKESVKTVTYESLNNIARCINGVSALLLTLLPGKANILEGLHGWELRPT 60

Query: 62  FRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGSR 121
           FRGPR PRWM NGVSSFN+FIHELSVDSD SSL+YSSGE+       P +PSSQSSR S 
Sbjct: 61  FRGPRLPRWMHNGVSSFNEFIHELSVDSDTSSLEYSSGEDS--DGPLPPSPSSQSSRLSW 120

Query: 122 SRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSP----GSP 181
           + +    +N  HWTE I  I  W++ P + LL IP      F+ + S   +SP     S 
Sbjct: 121 ASTSASSEN--HWTEWITFILWWLMFPVRILLWIPQYIISLFYKRSSRIPASPRRHQHSS 180

Query: 182 WPSIKRVHSHKEH-VVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIR 241
            P I + +S K+H V + TTDRRRGVIEDLHLA EI IE+ F+  HKA H +LSP +   
Sbjct: 181 RPRISKTNSSKDHDVPNRTTDRRRGVIEDLHLAIEIGIEAIFDFFHKATHLLLSPSEAFA 240

Query: 242 TLFRWLWSWSSCERDHDADV----IVPTATLGDDDPVPSERTY----TFHQSLNTDARTC 301
            L  W  S S   +++  +V     V TATLGD DP P+ER        + S+NTD RTC
Sbjct: 241 ILLSWFSSSSRSPKENHGEVAYDETVQTATLGDTDPSPTERPVESPTRLYNSMNTDTRTC 300

Query: 302 QDVITELGYPYEAIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSP 361
           QDVITELGYPYEAIRV+T+DGYVL+LERIPRRDARK ++LQHG+ DSSMGWVSNGVVGSP
Sbjct: 301 QDVITELGYPYEAIRVITSDGYVLVLERIPRRDARKAVFLQHGVLDSSMGWVSNGVVGSP 360

Query: 362 AFAAFDQGYDVYLGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTS 421
           AFAA+DQGYDV+LGNFRGLVSR+HV+KNISS+++W+YSINEH  EDIPAMIEKIHE+KT+
Sbjct: 361 AFAAYDQGYDVFLGNFRGLVSRDHVNKNISSKEFWRYSINEHGTEDIPAMIEKIHEIKTT 420

Query: 422 ELKLSQPELAEETDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGF 481
           ELKL QP + EE + ++P+KLCA+CHS+GGAA+LMYVITR+I+EKPHRLSRL+LLSPAGF
Sbjct: 421 ELKLCQPNIDEEINQEEPYKLCAICHSLGGAAILMYVITRKIKEKPHRLSRLILLSPAGF 480

Query: 482 HDDAPFIFTVIENLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTV 541
           H+D+   FT++E + L +SP+LA  VP  YIPTRFFRMLLNKLARDFH+YPA+GGLVQT+
Sbjct: 481 HEDSNLGFTIVEYIFLFISPVLARIVPAFYIPTRFFRMLLNKLARDFHNYPALGGLVQTL 540

Query: 542 VSYFLGGDSSNWVGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEA 601
           +SY +GGDSSNWVGVLG+PHYNMNDMP VSFRV  HLAQ+KH  KFRM+DYG+ S NME 
Sbjct: 541 MSYVVGGDSSNWVGVLGLPHYNMNDMPAVSFRVAQHLAQIKHTGKFRMYDYGSRSANMEV 600

Query: 602 YGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHL 661
           YGSPEPLDLGE +   D+PVDLVAGR D+VIR +MVK++Y +M+DA VDVSFNEFEYAHL
Sbjct: 601 YGSPEPLDLGESYKFIDVPVDLVAGRNDKVIRSSMVKKHYNVMRDAEVDVSFNEFEYAHL 660

Query: 662 DFTFSHREELLAYVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDK 695
           DFTFSHREELL YVMSRLLLV +    P  + + SQK  KL++  K
Sbjct: 661 DFTFSHREELLRYVMSRLLLVKQ---TPVQQRQTSQKGMKLKKKKK 699

BLAST of CmaCh13G010370 vs. TAIR 10
Match: AT1G18460.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 906.0 bits (2340), Expect = 1.9e-263
Identity = 464/702 (66.10%), Postives = 545/702 (77.64%), Query Frame = 0

Query: 2   IQRFVDDVLAVTKESVKTFTYESLNNVVRLINGLSALLLTLLPGRGSVLEGVHGWELRPT 61
           +QR VD+ LA+TKESVKT TYESLNN+ R ING SALLLTLLPG+ +VLEG+HGWELRPT
Sbjct: 1   MQRIVDNALAITKESVKTLTYESLNNIARCINGFSALLLTLLPGKSNVLEGLHGWELRPT 60

Query: 62  FRGPRFPRWMENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGSR 121
            RGPR PRWM NGVSSFN FIHELSVDSD SSLDYSSG++D  G   P +P SQSS   R
Sbjct: 61  LRGPRLPRWMHNGVSSFNHFIHELSVDSDTSSLDYSSGDDDSDGMSTPPSPLSQSS--LR 120

Query: 122 SRSGFHVKNVWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSS-SPG----S 181
           S +        HWT+ I  I  W LLPA+ LL +P         + S     SPG    S
Sbjct: 121 SWASLPANYESHWTDWITFIVWWALLPARILLWVPLYLLRLLARRNSRMQPLSPGRYQHS 180

Query: 182 PWPSIKRVHSHKEH-VVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTI 241
             P   +  S KEH V + TTD+RRGVIEDL L  EIFIE+ F+  HKAAH +LSP +T 
Sbjct: 181 SRPCFSKAISGKEHDVPNRTTDKRRGVIEDLQLGIEIFIETIFDFFHKAAHLLLSPSETF 240

Query: 242 RTLFRWLWSWSSCERDHDADV----IVPTATLGDDDPVPSERTYTFHQSLNTDARTCQDV 301
             +  W  S S   + +  DV    I+ TA LGD+D   +ER  T     NTD RTCQDV
Sbjct: 241 GIVLSWFSSSSHSSKGNYGDVSDDEIIQTAILGDNDSSLTERR-TTTSLYNTDTRTCQDV 300

Query: 302 ITELGYPYEAIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFA 361
           ITELGYPYEAIRVVT+DGY LLLERIPRRDARK +YLQHG+ DSSMGWVSNGVVGSPAFA
Sbjct: 301 ITELGYPYEAIRVVTSDGYGLLLERIPRRDARKAVYLQHGVMDSSMGWVSNGVVGSPAFA 360

Query: 362 AFDQGYDVYLGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELK 421
           A+DQGYDV+LGNFRGLVSR+HV KNISS+ +W+YSINEHA EDIPAMIEKIHE+KTSELK
Sbjct: 361 AYDQGYDVFLGNFRGLVSRDHVKKNISSKDFWRYSINEHATEDIPAMIEKIHEIKTSELK 420

Query: 422 LSQPELAEETDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDD 481
           L QP + E  + DQP+KLC + HS+GGAA+LMYVITR+IEEKPHRLSRL+LLSPAGFH D
Sbjct: 421 LYQPTMEEVVNEDQPYKLCVVSHSLGGAAVLMYVITRKIEEKPHRLSRLILLSPAGFHYD 480

Query: 482 APFIFTVIENLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSY 541
           +   FT++E   L L P+L+  VP  YIPT+FFRMLLNKLARDFH+YPAVGGLVQT++SY
Sbjct: 481 SNMCFTLMEYTFLFLGPVLSRIVPAFYIPTKFFRMLLNKLARDFHNYPAVGGLVQTLMSY 540

Query: 542 FLGGDSSNWVGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGS 601
            +GGDSSNWVGV+G+PHYNMNDMPG+SFRV  HLAQ+KH+ KF+MFDYG++S NM+ YGS
Sbjct: 541 VVGGDSSNWVGVMGLPHYNMNDMPGISFRVAQHLAQIKHSGKFKMFDYGSSSANMDVYGS 600

Query: 602 PEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFT 661
           PEPLDLGE++GL D+PVDLVAG+KD+VIRP+MV+++Y++M+D+GVDVS+NEFEYAHLDFT
Sbjct: 601 PEPLDLGEFYGLIDVPVDLVAGKKDKVIRPSMVRKHYRVMRDSGVDVSYNEFEYAHLDFT 660

Query: 662 FSHREELLAYVMSRLLLVGEPGS-----KPKLKLKPSQKVPK 689
           FSHREELLAYVMSRLLLV EP       K  +KLK   +  K
Sbjct: 661 FSHREELLAYVMSRLLLV-EPTQTQTVHKKGMKLKKKMETGK 698

BLAST of CmaCh13G010370 vs. TAIR 10
Match: AT1G73920.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 804.7 bits (2077), Expect = 6.0e-233
Identity = 410/637 (64.36%), Postives = 494/637 (77.55%), Query Frame = 0

Query: 71  MENGVSSFNQFIHELSVDSDDSSLDYSSGEEDGIGNEYPATPSSQSSRGSRSRSGFHVKN 130
           M NGVSSFN+FIHELSVDSD SSL+YSSGE+       P +PSSQSSR S + +    +N
Sbjct: 1   MHNGVSSFNEFIHELSVDSDTSSLEYSSGEDS--DGPLPPSPSSQSSRLSWASTSASSEN 60

Query: 131 VWHWTERIRSIFLWILLPAKFLLGIPFRTFHFFFIKWSGSSSSP----GSPWPSIKRVHS 190
             HWTE I  I  W++ P + LL IP      F+ + S   +SP     S  P I + +S
Sbjct: 61  --HWTEWITFILWWLMFPVRILLWIPQYIISLFYKRSSRIPASPRRHQHSSRPRISKTNS 120

Query: 191 HKEH-VVHSTTDRRRGVIEDLHLASEIFIESAFEMVHKAAHFILSPLDTIRTLFRWLWSW 250
            K+H V + TTDRRRGVIEDLHLA EI IE+ F+  HKA H +LSP +    L  W  S 
Sbjct: 121 SKDHDVPNRTTDRRRGVIEDLHLAIEIGIEAIFDFFHKATHLLLSPSEAFAILLSWFSSS 180

Query: 251 SSCERDHDADV----IVPTATLGDDDPVPSERTY----TFHQSLNTDARTCQDVITELGY 310
           S   +++  +V     V TATLGD DP P+ER        + S+NTD RTCQDVITELGY
Sbjct: 181 SRSPKENHGEVAYDETVQTATLGDTDPSPTERPVESPTRLYNSMNTDTRTCQDVITELGY 240

Query: 311 PYEAIRVVTNDGYVLLLERIPRRDARKVLYLQHGIFDSSMGWVSNGVVGSPAFAAFDQGY 370
           PYEAIRV+T+DGYVL+LERIPRRDARK ++LQHG+ DSSMGWVSNGVVGSPAFAA+DQGY
Sbjct: 241 PYEAIRVITSDGYVLVLERIPRRDARKAVFLQHGVLDSSMGWVSNGVVGSPAFAAYDQGY 300

Query: 371 DVYLGNFRGLVSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKIHEVKTSELKLSQPEL 430
           DV+LGNFRGLVSR+HV+KNISS+++W+YSINEH  EDIPAMIEKIHE+KT+ELKL QP +
Sbjct: 301 DVFLGNFRGLVSRDHVNKNISSKEFWRYSINEHGTEDIPAMIEKIHEIKTTELKLCQPNI 360

Query: 431 AEETDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRLSRLVLLSPAGFHDDAPFIFT 490
            EE + ++P+KLCA+CHS+GGAA+LMYVITR+I+EKPHRLSRL+LLSPAGFH+D+   FT
Sbjct: 361 DEEINQEEPYKLCAICHSLGGAAILMYVITRKIKEKPHRLSRLILLSPAGFHEDSNLGFT 420

Query: 491 VIENLLLLLSPLLAPFVPGLYIPTRFFRMLLNKLARDFHHYPAVGGLVQTVVSYFLGGDS 550
           ++E + L +SP+LA  VP  YIPTRFFRMLLNKLARDFH+YPA+GGLVQT++SY +GGDS
Sbjct: 421 IVEYIFLFISPVLARIVPAFYIPTRFFRMLLNKLARDFHNYPALGGLVQTLMSYVVGGDS 480

Query: 551 SNWVGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKKFRMFDYGNASLNMEAYGSPEPLDL 610
           SNWVGVLG+PHYNMNDMP VSFRV  HLAQ+KH  KFRM+DYG+ S NME YGSPEPLDL
Sbjct: 481 SNWVGVLGLPHYNMNDMPAVSFRVAQHLAQIKHTGKFRMYDYGSRSANMEVYGSPEPLDL 540

Query: 611 GEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKDAGVDVSFNEFEYAHLDFTFSHREE 670
           GE +   D+PVDLVAGR D+VIR +MVK++Y +M+DA VDVSFNEFEYAHLDFTFSHREE
Sbjct: 541 GESYKFIDVPVDLVAGRNDKVIRSSMVKKHYNVMRDAEVDVSFNEFEYAHLDFTFSHREE 600

Query: 671 LLAYVMSRLLLVGEPGSKPKLKLKPSQKVPKLRRNDK 695
           LL YVMSRLLLV +    P  + + SQK  KL++  K
Sbjct: 601 LLRYVMSRLLLVKQ---TPVQQRQTSQKGMKLKKKKK 630

BLAST of CmaCh13G010370 vs. TAIR 10
Match: AT5G14180.1 (Myzus persicae-induced lipase 1 )

HSP 1 Score: 89.0 bits (219), Expect = 1.7e-17
Identity = 92/379 (24.27%), Postives = 150/379 (39.58%), Query Frame = 0

Query: 288 CQDVITELGYPYEAIRVVTNDGYVLLLERIPRRDA---------RKVLYLQHGIFDSSMG 347
           C   +   GY  E   VVT DGY+L ++RIP   A         R+ + +QHGI    M 
Sbjct: 52  CASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDGMS 111

Query: 348 WVSNGVVGSPAFAAFDQGYDVYLGNFRGL-VSREHVDKNISSRQYWKYSINEHAMEDIPA 407
           W+ N    +      DQG+DV++GN RG   SR H   N S R +W ++ +E    D+PA
Sbjct: 112 WLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYDLPA 171

Query: 408 MIEKIHEVKTSELKLSQPELAEETDSDQPFKLCALCHSMGGAAMLMYVITRRIEEKPHRL 467
           M + IH +                      K+  L HS+G          + + +   ++
Sbjct: 172 MFDHIHGLTGQ-------------------KIHYLGHSLGTLIGFASFSEKGLVD---QV 231

Query: 468 SRLVLLSPAGFHDDAPFIFTVI--ENLLLLLSPLLA--PFVPGLYIPTRFFRMLLNKLAR 527
               +LSP  +      +   I  +  L   + +L    F P   +   F + +  K   
Sbjct: 232 RSAAMLSPVAYLSHMTTVIGDIAAKTFLAEATSILGWPEFNPKSGLVGDFIKAICLKAGI 291

Query: 528 DFHHYPAVGGLVQTVVSYFLGGDSSNWVGVLGIPHYNMNDMPGVSFRVGLHLAQMKHAKK 587
           D +           +VS   G +    +    I  +  N+    S +  +HLAQ    K+
Sbjct: 292 DCY----------DLVSVITGKNCC--LNASTIDLFLANEPQSTSTKNMIHLAQTVRDKE 351

Query: 588 FRMFDYGNASLNMEAYGSPEPLDLGEYFGLFDIPVDLVAGRKDQVIRPTMVKRYYKMMKD 647
            R ++YG++  N++ YG   P          ++P+    G  D +     V+      K 
Sbjct: 352 LRKYNYGSSDRNIKHYGQAIPPAYNISAIPHELPLFFSYGGLDSLADVKDVEFLLDQFKY 396

Query: 648 AGVDVSFNEF--EYAHLDF 651
             +D    +F  +YAH DF
Sbjct: 412 HDIDKMNVQFVKDYAHADF 396

BLAST of CmaCh13G010370 vs. TAIR 10
Match: AT2G15230.1 (lipase 1 )

HSP 1 Score: 75.5 bits (184), Expect = 1.9e-13
Identity = 46/155 (29.68%), Postives = 76/155 (49.03%), Query Frame = 0

Query: 288 CQDVITELGYPYEAIRVVTNDGYVLLLERI----PRRDARKVLYLQHGIFDSSMGWVSNG 347
           C D+I    Y      + T DGY+L L+R+    PR  +   + LQHG+F +   W  N 
Sbjct: 33  CADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRLQSGPPVLLQHGLFMAGDVWFLNS 92

Query: 348 VVGSPAFAAFDQGYDVYLGNFRGL-VSREHVDKNISSRQYWKYSINEHAMEDIPAMIEKI 407
              S  F   D G+DV++GN RG   S  HV  + + +++W +S  + AM D+  MI+ +
Sbjct: 93  PKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEFWDWSWQDLAMYDLAEMIQYL 152

Query: 408 HEVKTSEL--------------KLSQPELAEETDS 424
           + +  S++               L+QP +AE  ++
Sbjct: 153 YSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEA 187

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5VYY21.3e-2226.39Lipase member M OS=Homo sapiens OX=9606 GN=LIPM PE=2 SV=2[more]
Q8K2A61.7e-2227.01Lipase member M OS=Mus musculus OX=10090 GN=Lipm PE=2 SV=1[more]
Q3U4B42.9e-2226.79Lipase member N OS=Mus musculus OX=10090 GN=Lipn PE=2 SV=1[more]
O461084.9e-2227.64Lipase 3 OS=Drosophila melanogaster OX=7227 GN=Lip3 PE=2 SV=1[more]
P800354.9e-2228.95Gastric triacylglycerol lipase OS=Canis lupus familiaris OX=9615 GN=LIPF PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1KNX80.0e+00100.00uncharacterized protein LOC111495192 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1EB220.0e+0099.71uncharacterized protein LOC111431539 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A1S3C3R60.0e+0090.87uncharacterized protein LOC103496491 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0LQR40.0e+0091.25Abhydro_lipase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042... [more]
A0A6J1KL850.0e+0092.83lipase member N isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495192 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_023000888.10.0e+00100.00uncharacterized protein LOC111495192 isoform X1 [Cucurbita maxima][more]
KAG6584371.10.0e+0099.86Lipase member N, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019955.1 L... [more]
XP_022923983.10.0e+0099.71uncharacterized protein LOC111431539 isoform X1 [Cucurbita moschata][more]
XP_023519485.10.0e+0099.43uncharacterized protein LOC111782880 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_038894357.10.0e+0092.84uncharacterized protein LOC120082972 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G73920.12.8e-26765.72alpha/beta-Hydrolases superfamily protein [more]
AT1G18460.11.9e-26366.10alpha/beta-Hydrolases superfamily protein [more]
AT1G73920.26.0e-23364.36alpha/beta-Hydrolases superfamily protein [more]
AT5G14180.11.7e-1724.27Myzus persicae-induced lipase 1 [more]
AT2G15230.11.9e-1329.68lipase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006693Partial AB-hydrolase lipase domainPFAMPF04083Abhydro_lipasecoord: 289..343
e-value: 4.7E-13
score: 48.5
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 276..667
e-value: 7.5E-81
score: 273.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 286..664
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 89..123
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 674..697
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 108..123
NoneNo IPR availablePANTHERPTHR11005LYSOSOMAL ACID LIPASE-RELATEDcoord: 9..677
NoneNo IPR availablePANTHERPTHR11005:SF134ALPHA/BETA HYDROLASE FAMILY PROTEINcoord: 9..677

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh13G010370.1CmaCh13G010370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016787 hydrolase activity