CmaCh13G009770 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh13G009770
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionDihydrothymine dehydrogenase
LocationCma_Chr13: 7608693 .. 7611858 (+)
RNA-Seq ExpressionCmaCh13G009770
SyntenyCmaCh13G009770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGAATACGTTTATAAATCCAGAAGGAAATTGACGAGGATGATTCTTCATGCCCCACCTTTCCAGCTTTCTTGATCGAATATTACATCTCAGATTGAGTTACCCATCTTCTTCTTCTCCTTCCACATCATTTTTTTGTTCCATTACAGGATTTTCTGTTGCAGAGAGCTTGAATTTCTTGATCCATGGCGTCGTTGGATTTTACCCAGATCAGAACTAGAAACCCAATGTCCGAGCTCGCGCTGAATCGGTCTCGGATGCGTTTGACTCGTCGAAATAGAGTTGGGTTCAGAGTGGTCGCCGCCGGTGAAGGTCAGGCGGAGCCTGATCTCAGTGTGACGGTCAATGGGTTGCACATGCCCAACCCGTTTGTAATTGGGTCGGGTCCGCCGGGAACTAACTATACGGTTATGAAGAGGGCATTTGATGAAGGTTGGGGCGCTGTGATTGCCAAAACTGTAAGTTGTCTCGCTTTTGATTTCGATTTCGTAGTCTGTTGATTCCGTTAGCTTATAATTTGATTTTTGAATGGCAAATTGGTTGCTGTTTGTGTAATCTACTGATCTGTGATTTCATCTTAGAGATTGTTCTTGATTAGGAATATGATTAGTGTTTGGAGAAAGAGATATTTAGATTGATCTTTGTCTGGAAGAACATTACTGGTTCTGCTATCTGTTCTTGATTGGCTGGAAGAACATTACTGGTTTTGCTATCTGTTCTTCATTGGCTGGCATGTACATTTCTTGTATTTTCATGGATCTTTGTCTCTGTTAGGTGTCACTAGATGCAGCAAAAGTAATAAATGTAACGCCTAGATATGCCCGGCTTCGAGCAGATTCAAGCGGCTCTGCAAAAGGGCAGATCATTGGGTGGGAGAACATTGAATTGATAAGCGACAGACCCCTTGAAACTATGTTGAAAGAGTTTAAGCAATTGAAAGAGGAGTATCCTGATAGGATTCTCATTGCTTCAATAATGGAGGAGTATAACAAAGCTGCTTGGGAAGAACTTATAGATCGAGTCGAACAAACCGGAGTTGTATGCCTACGATCTATGCCTTCTTTACCGTGTTTATTACCAAGAACAGTTCATTTCATTGAATGTTTATGTCTCTTACTTAAAGGATGCATTTGAAATCAATTTCTCGTGCCCTCACGGCATGCCCGAACGGAGAATGGGTGCTGCAGTCGGGCAAGATTGTGCGCTACTGGAAGAGGTTTGTGGATGGATAAATGCTAAAGCTACAATCCCTGTCTGGGCTAAAATGACTCCCAATATCACTGACATTTCACAGGTAGTTTTGCTGTTCTTCTATTTGAATGAGTATCTATGGTTGCATGAACTGATGCAACGTACAATTCTTCTTTTGTAAAGCCTGCTAGGGTGGCTCTGAAATTGGGATGCGAGGGGATAGCGGCTATCAATACGATCATGAGTGTAATGGGAATCGATCTCAAGACCTTACGCCCTGAGCCTTGTGTTGAGGGGTTTGCTAATTCGAACTTTTGAACTCGTCGATATAGAATGATTTTGTGTTTATTAGCATTCAGCTGAAATGTTGGATGTGGTTCAATCTGTATGTTGTTAGGTATTCAACTCCAGGAGGCTACTCTTCCAAGGCAGTTCATCCTATTGCTCTTGGTAAAGTGTTGAACATTGCAAAAATGATGAAGGCTGAATTTAATGATGCAGACTACTCTCTTTCTGGCATTGGAGGTGTCGAAACCGGTGGAGATGCTGCCGAGTTTATTCTACTCGGAGCAAACACTGTTCAGGTACAACGAGAAATTTGTAACCGTCCAAGAACGTGTCTGCTAGGGAGTGGTTTCTAAACTCTTATAAAGAATGTTTTGTTCCCTTTTTAAACATATGTGAGATCTCATAATCCCCTCCCGCCTAGGGAGCTAGCATCCTCACTGGCACACCGCCCAATGACTGATTCTGATACCATTTTAACTGCCCAAGTCCACCGCCAGTAGTAATGTCCTCTTTGAGCTTTCTCCTCTGGGTTTCCCCTCAAGGTTTTAAAACGTGTCAGGGAGAGGTTTCCTCACCTTCTACGGGTCGTCCCCTCTCCAACCGATGTAAGATCTTACAATCCCCATTGGGGGCCCAACATCCTTGCTAGCACGCCGCCCGATGACGAGCTCTAGTATGGTAACAGTTCAAGCCCATCGCTAGCAGATATTGTCATATTTGGGTTTTCCCTTCGGGCTTCTCCGGCCTTTCCTTTTTGGGCTTCCCCTCAAGTTTTTAAAACACGTTGGGGGAGAGGTTTCCACAATCTTACAAGGAATGTTTCGTTCCCTAAGATCTTACAAAAAATTTTTCCTCTCCAATCGATGTGAGATCTCACCAAATTATAGCCTATAATTTATGTTGCGAAATCGACTAGTTCATTTAACGAAACTGGTCGTAATGGCTCGGACATCCTTTTGTTTTAGGATATTCCTAATGGTGTACTATACTGCATTGTTGTATGCTGAGGAAGTTGCATTCATATGCAGGTGTGCACGGGAGTGATGATGCATGGTTATGGCCTCGTTAAGAAACTCTGTGCTGAGCTGAAGGACTTCATGAAACTTCACAACTTCTCATCCATCGAAGAGTTCAGAGGGTAAGTTTGGAGTTTGAAATCAATAGCAGTTCTTAATAGAACACGGCTCGATCCCCTCGAACATCGGTCACACTAACGAATTCCGGGTGAAACAGGGCTTCCCTTCCGTACTTCACAACGCACACGGATTTAGTTCGGAGGCAGCGGGAAGCAATCGAGCAGAGAAAAGCTGTGAAGAAGGGTCTGCAATCTGATAAAGATTGGACTGGCGATGGCTTTGTCAAGGAAACTGAGAGTATGGTTTCAAACTGAAGGGCTTAATACAGTACACGAATCAAGCCTTTGCTTTGGCTTGGCAGTAGAAATAACTCAACATGGTGTCTTTGTTACATCCGAGTTAAGTTTCTTTTGTTCTTTCTTTCTCCTTCCCTCGAATAAGTGTATCATTACAGAACAGTTTTAATTTCGTATGATTGTGTATATGTGAAGGGATATTAGCAATATTTCTATTGAAATTCCTTTCCAAGATCAATAATCTTAGAAATTCATAATCAAATCATGTACGAATCTCAACAGAATCCAAAAATAATTCACGAATACTTCAGATATT

mRNA sequence

TTGAATACGTTTATAAATCCAGAAGGAAATTGACGAGGATGATTCTTCATGCCCCACCTTTCCAGCTTTCTTGATCGAATATTACATCTCAGATTGAGTTACCCATCTTCTTCTTCTCCTTCCACATCATTTTTTTGTTCCATTACAGGATTTTCTGTTGCAGAGAGCTTGAATTTCTTGATCCATGGCGTCGTTGGATTTTACCCAGATCAGAACTAGAAACCCAATGTCCGAGCTCGCGCTGAATCGGTCTCGGATGCGTTTGACTCGTCGAAATAGAGTTGGGTTCAGAGTGGTCGCCGCCGGTGAAGGTCAGGCGGAGCCTGATCTCAGTGTGACGGTCAATGGGTTGCACATGCCCAACCCGTTTGTAATTGGGTCGGGTCCGCCGGGAACTAACTATACGGTTATGAAGAGGGCATTTGATGAAGGTTGGGGCGCTGTGATTGCCAAAACTGTGTCACTAGATGCAGCAAAAGTAATAAATGTAACGCCTAGATATGCCCGGCTTCGAGCAGATTCAAGCGGCTCTGCAAAAGGGCAGATCATTGGGTGGGAGAACATTGAATTGATAAGCGACAGACCCCTTGAAACTATGTTGAAAGAGTTTAAGCAATTGAAAGAGGAGTATCCTGATAGGATTCTCATTGCTTCAATAATGGAGGAGTATAACAAAGCTGCTTGGGAAGAACTTATAGATCGAGTCGAACAAACCGGAGTTGATGCATTTGAAATCAATTTCTCGTGCCCTCACGGCATGCCCGAACGGAGAATGGGTGCTGCAGTCGGGCAAGATTGTGCGCTACTGGAAGAGGTTTGTGGATGGATAAATGCTAAAGCTACAATCCCTGTCTGGGCTAAAATGACTCCCAATATCACTGACATTTCACAGCCTGCTAGGGTGGCTCTGAAATTGGGATGCGAGGGGATAGCGGCTATCAATACGATCATGAGTGTAATGGGAATCGATCTCAAGACCTTACGCCCTGAGCCTTGTGTTGAGGGGTATTCAACTCCAGGAGGCTACTCTTCCAAGGCAGTTCATCCTATTGCTCTTGGTAAAGTGTTGAACATTGCAAAAATGATGAAGGCTGAATTTAATGATGCAGACTACTCTCTTTCTGGCATTGGAGGTGTGTGCACGGGAGTGATGATGCATGGTTATGGCCTCGTTAAGAAACTCTGTGCTGAGCTGAAGGACTTCATGAAACTTCACAACTTCTCATCCATCGAAGAGTTCAGAGGGGCTTCCCTTCCGTACTTCACAACGCACACGGATTTAGTTCGGAGGCAGCGGGAAGCAATCGAGCAGAGAAAAGCTGTGAAGAAGGGTCTGCAATCTGATAAAGATTGGACTGGCGATGGCTTTGTCAAGGAAACTGAGAGTATGGTTTCAAACTGAAGGGCTTAATACAGTACACGAATCAAGCCTTTGCTTTGGCTTGGCAGTAGAAATAACTCAACATGGTGTCTTTGTTACATCCGAGTTAAGTTTCTTTTGTTCTTTCTTTCTCCTTCCCTCGAATAAGTGTATCATTACAGAACAGTTTTAATTTCGTATGATTGTGTATATGTGAAGGGATATTAGCAATATTTCTATTGAAATTCCTTTCCAAGATCAATAATCTTAGAAATTCATAATCAAATCATGTACGAATCTCAACAGAATCCAAAAATAATTCACGAATACTTCAGATATT

Coding sequence (CDS)

ATGGCGTCGTTGGATTTTACCCAGATCAGAACTAGAAACCCAATGTCCGAGCTCGCGCTGAATCGGTCTCGGATGCGTTTGACTCGTCGAAATAGAGTTGGGTTCAGAGTGGTCGCCGCCGGTGAAGGTCAGGCGGAGCCTGATCTCAGTGTGACGGTCAATGGGTTGCACATGCCCAACCCGTTTGTAATTGGGTCGGGTCCGCCGGGAACTAACTATACGGTTATGAAGAGGGCATTTGATGAAGGTTGGGGCGCTGTGATTGCCAAAACTGTGTCACTAGATGCAGCAAAAGTAATAAATGTAACGCCTAGATATGCCCGGCTTCGAGCAGATTCAAGCGGCTCTGCAAAAGGGCAGATCATTGGGTGGGAGAACATTGAATTGATAAGCGACAGACCCCTTGAAACTATGTTGAAAGAGTTTAAGCAATTGAAAGAGGAGTATCCTGATAGGATTCTCATTGCTTCAATAATGGAGGAGTATAACAAAGCTGCTTGGGAAGAACTTATAGATCGAGTCGAACAAACCGGAGTTGATGCATTTGAAATCAATTTCTCGTGCCCTCACGGCATGCCCGAACGGAGAATGGGTGCTGCAGTCGGGCAAGATTGTGCGCTACTGGAAGAGGTTTGTGGATGGATAAATGCTAAAGCTACAATCCCTGTCTGGGCTAAAATGACTCCCAATATCACTGACATTTCACAGCCTGCTAGGGTGGCTCTGAAATTGGGATGCGAGGGGATAGCGGCTATCAATACGATCATGAGTGTAATGGGAATCGATCTCAAGACCTTACGCCCTGAGCCTTGTGTTGAGGGGTATTCAACTCCAGGAGGCTACTCTTCCAAGGCAGTTCATCCTATTGCTCTTGGTAAAGTGTTGAACATTGCAAAAATGATGAAGGCTGAATTTAATGATGCAGACTACTCTCTTTCTGGCATTGGAGGTGTGTGCACGGGAGTGATGATGCATGGTTATGGCCTCGTTAAGAAACTCTGTGCTGAGCTGAAGGACTTCATGAAACTTCACAACTTCTCATCCATCGAAGAGTTCAGAGGGGCTTCCCTTCCGTACTTCACAACGCACACGGATTTAGTTCGGAGGCAGCGGGAAGCAATCGAGCAGAGAAAAGCTGTGAAGAAGGGTCTGCAATCTGATAAAGATTGGACTGGCGATGGCTTTGTCAAGGAAACTGAGAGTATGGTTTCAAACTGA

Protein sequence

MASLDFTQIRTRNPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPNPFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSSGSAKGQIIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVDAFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARVALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKMMKAEFNDADYSLSGIGGVCTGVMMHGYGLVKKLCAELKDFMKLHNFSSIEEFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETESMVSN
Homology
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BLAST of CmaCh13G009770 vs. ExPASy Swiss-Prot
Match: Q9LVI9 (Dihydropyrimidine dehydrogenase (NADP(+)), chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PYD1 PE=1 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 6.9e-186
Identity = 331/429 (77.16%), Postives = 362/429 (84.38%), Query Frame = 0 

Query: 1   MASLDFTQIRTRNPMSELALN-----RSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNG 60
           MAS+ F   R     S+  L+      SR       RVG ++ +A E  +EPDLSVTVNG
Sbjct: 1   MASMSFALNRFSGLSSKTTLSADFDPSSRRSFLPPTRVGLKISSAAE--SEPDLSVTVNG 60

Query: 61  LHMPNPFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSSG 120
           L MPNPFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDA+KVINVTPRYARLR  S+G
Sbjct: 61  LKMPNPFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDASKVINVTPRYARLRTGSNG 120

Query: 121 SAKGQIIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVE 180
           SAK  +IGW+NIELISDRPLETMLKEF++LK+EYPDRILIAS+MEEYNK AWEELIDRVE
Sbjct: 121 SAKTDVIGWQNIELISDRPLETMLKEFERLKKEYPDRILIASVMEEYNKTAWEELIDRVE 180

Query: 181 QTGVDAFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDIS 240
           QTGVDA EINFSCPHGMPERRMGAAVGQDCALL+EVCGWINAKAT+PVWAKMTPNITDI+
Sbjct: 181 QTGVDALEINFSCPHGMPERRMGAAVGQDCALLDEVCGWINAKATVPVWAKMTPNITDIT 240

Query: 241 QPARVALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVL 300
           +PARV+LK GCEGIAAINTIMSVMGID+KTLRPEPCVEGYSTPGGYS KAV PIAL KV+
Sbjct: 241 EPARVSLKSGCEGIAAINTIMSVMGIDMKTLRPEPCVEGYSTPGGYSYKAVRPIALAKVM 300

Query: 301 NIAKMMKAEFNDADYSLSGIGG-------------------VCTGVMMHGYGLVKKLCAE 360
           NIAKMMK+EF++ D SLSGIGG                   VCTGVMMHGYG VK LCAE
Sbjct: 301 NIAKMMKSEFSE-DRSLSGIGGVETGYDAAEFILLGSNTVQVCTGVMMHGYGHVKTLCAE 360

Query: 361 LKDFMKLHNFSSIEEFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFV 406
           LKDFMK HNFS+IEEFRG SL YFTTHTDLV+RQ+EA+EQRKA K+GL+SDKDWTGDGFV
Sbjct: 361 LKDFMKQHNFSTIEEFRGHSLQYFTTHTDLVKRQKEAVEQRKAEKRGLKSDKDWTGDGFV 420

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BLAST of CmaCh13G009770 vs. ExPASy Swiss-Prot
Match: Q8X643 (NAD-dependent dihydropyrimidine dehydrogenase subunit PreA OS=Escherichia coli O157:H7 OX=83334 GN=preA PE=3 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 5.5e-58
Identity = 132/340 (38.82%), Postives = 186/340 (54.71%), Query Frame = 0 

Query: 48  DLSVTVNGLHMPNPFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYA 107
           DLS+T  G+  PNPF + S P G  Y +  +A+D GWG V+ KT+    A    V+PR+ 
Sbjct: 7   DLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGVVFKTIGFFIAN--EVSPRFD 66

Query: 108 RLRADSSGSAKGQIIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAW 167
            L  + +G      IG++N+E I++ PLE  L   ++LKE+YPD++LIASIM E N+  W
Sbjct: 67  HLVKEDTG-----FIGFKNMEQIAEHPLEENLAALRRLKEDYPDKVLIASIMGE-NEQQW 126

Query: 168 EELIDRVEQTGVDAFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKM 227
           EEL   V++ G D  E NFSCP  M    MG+ VGQ   L+E+ C  +   +T+P+ AKM
Sbjct: 127 EELARLVQEAGADMIECNFSCPQ-MTSHAMGSDVGQSPELVEKYCRAVKRGSTLPMLAKM 186

Query: 228 TPNITDISQPARVALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVH 287
           TPNI D+ + A  A + G +GIAAINT+ S+  IDL      P V G S+  GYS KAV 
Sbjct: 187 TPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMPIVNGKSSISGYSGKAVK 246

Query: 288 PIALGKVLNIAKMMKAEFNDADYSLSGIGG-------------------VCTGVMMHGYG 347
           PIA    L   + M+      D+ +SGIGG                   V TG+M +GY 
Sbjct: 247 PIA----LRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGAATLQVTTGIMQYGYR 306

Query: 348 LVKKLCAELKDFMKLHNFSSIEEFRGASLPYFTTHTDLVR 369
           +V+ + + L  ++    F S++E  G +        DL R
Sbjct: 307 IVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDR 333

[Close]
BLAST of CmaCh13G009770 vs. ExPASy Swiss-Prot
Match: P25889 (NAD-dependent dihydropyrimidine dehydrogenase subunit PreA OS=Escherichia coli (strain K12) OX=83333 GN=preA PE=1 SV=3)

HSP 1 Score: 226.5 bits (576), Expect = 5.5e-58
Identity = 132/340 (38.82%), Postives = 186/340 (54.71%), Query Frame = 0 

Query: 48  DLSVTVNGLHMPNPFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYA 107
           DLS+T  G+  PNPF + S P G  Y +  +A+D GWG V+ KT+    A    V+PR+ 
Sbjct: 5   DLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGVVFKTIGFFIAN--EVSPRFD 64

Query: 108 RLRADSSGSAKGQIIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAW 167
            L  + +G      IG++N+E I++ PLE  L   ++LKE+YPD++LIASIM E N+  W
Sbjct: 65  HLVKEDTG-----FIGFKNMEQIAEHPLEENLAALRRLKEDYPDKVLIASIMGE-NEQQW 124

Query: 168 EELIDRVEQTGVDAFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKM 227
           EEL   V++ G D  E NFSCP  M    MG+ VGQ   L+E+ C  +   +T+P+ AKM
Sbjct: 125 EELARLVQEAGADMIECNFSCPQ-MTSHAMGSDVGQSPELVEKYCRAVKRGSTLPMLAKM 184

Query: 228 TPNITDISQPARVALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVH 287
           TPNI D+ + A  A + G +GIAAINT+ S+  IDL      P V G S+  GYS KAV 
Sbjct: 185 TPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMPIVNGKSSISGYSGKAVK 244

Query: 288 PIALGKVLNIAKMMKAEFNDADYSLSGIGG-------------------VCTGVMMHGYG 347
           PIA    L   + M+      D+ +SGIGG                   V TG+M +GY 
Sbjct: 245 PIA----LRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGAATLQVTTGIMQYGYR 304

Query: 348 LVKKLCAELKDFMKLHNFSSIEEFRGASLPYFTTHTDLVR 369
           +V+ + + L  ++    F S++E  G +        DL R
Sbjct: 305 IVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDR 331

[Close]
BLAST of CmaCh13G009770 vs. ExPASy Swiss-Prot
Match: Q8ZNL7 (NAD-dependent dihydropyrimidine dehydrogenase subunit PreA OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=preA PE=3 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 2.3e-56
Identity = 125/326 (38.34%), Postives = 182/326 (55.83%), Query Frame = 0 

Query: 48  DLSVTVNGLHMPNPFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYA 107
           DLSVT  G+  PNPF + S P G  Y +  +A+D GWG ++ KT+    A    V+PR+ 
Sbjct: 5   DLSVTFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGIVFKTIGFFIAN--EVSPRFD 64

Query: 108 RLRADSSGSAKGQIIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAW 167
            L  + +G      IG++N+E I++ PLE  L   ++LK++YPD++LIASIM E N+  W
Sbjct: 65  HLTKEDTG-----FIGFKNMEQIAEHPLEENLAAIRRLKQDYPDKVLIASIMGE-NEQQW 124

Query: 168 EELIDRVEQTGVDAFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKM 227
           +EL   VE+ G D  E NFSCP  M    MG+ VGQ   L+E+ C  +   +++P+ AKM
Sbjct: 125 QELARLVEEAGADMIECNFSCPQ-MTSHAMGSDVGQSPELVEKYCRAVKRGSSLPMLAKM 184

Query: 228 TPNITDISQPARVALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVH 287
           TPNI D+ + A  A + G +GIA INT+ S+  IDL      P V G S+  GYS KAV 
Sbjct: 185 TPNIGDMCEVALAAKRGGADGIATINTVKSITNIDLNRKIGMPVVNGKSSISGYSGKAVK 244

Query: 288 PIALGKVLNIAKMMKAEFNDADYSLSGIGG-------------------VCTGVMMHGYG 347
           PIA    L   + ++      D+ +SGIGG                   V TG+M +GY 
Sbjct: 245 PIA----LRFIQQLRMHPELRDFPISGIGGIETWEDAAEFLLLGAATLQVTTGIMQYGYR 304

Query: 348 LVKKLCAELKDFMKLHNFSSIEEFRG 355
           +V+ + + L  ++    F+S++E  G
Sbjct: 305 IVEDMASGLSHYLADQGFASLQEMIG 317

[Close]
BLAST of CmaCh13G009770 vs. ExPASy Swiss-Prot
Match: Q8CHR6 (Dihydropyrimidine dehydrogenase [NADP(+)] OS=Mus musculus OX=10090 GN=Dpyd PE=1 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 9.7e-55
Identity = 122/330 (36.97%), Postives = 177/330 (53.64%), Query Frame = 0 

Query: 48  DLSVTVNGLHMPNPFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYA 107
           D+SV + GL  PNPF + S  P T+  +++RAF+ GWG  + KT SLD   V NV+PR  
Sbjct: 532 DISVEMAGLRFPNPFGLASATPATSTPMIRRAFEAGWGFALTKTFSLDKDIVTNVSPRII 591

Query: 108 RLRADSSGSAKGQIIG-----WENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEY 167
           R      G+  G + G     + NIELIS++          +LK ++PD ILIASIM  Y
Sbjct: 592 R------GTTSGPLYGPGQSSFLNIELISEKTAAYWCHSVTELKADFPDNILIASIMCSY 651

Query: 168 NKAAWEELIDRVEQTGVDAFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIP 227
           NK+ W EL    E +G DA E+N SCPHGM ER MG A GQD  L+  +C W+     +P
Sbjct: 652 NKSDWMELSKMAEASGADALELNLSCPHGMGERGMGLACGQDPELVRNICRWVRQAVRVP 711

Query: 228 VWAKMTPNITDISQPARVALKLGCEGIAAINTIMSVMGIDLK-TLRPEPCVEGYSTPGGY 287
            +AK+TPN+TDI   AR A + G +G+ A NT+  +MG+    T  P   +   +T GG 
Sbjct: 712 FFAKLTPNVTDIVSIARAAKEGGADGVTATNTVSGLMGLKADGTPWPAVGIGRRTTYGGV 771

Query: 288 SSKAVHPIALGKVLNIAK-------MMKAEFNDAD------YSLSGIGGVCTGVMMHGYG 347
           S  A+ PIAL  V  IA+       +     + A+      +S + +  VC+ +    + 
Sbjct: 772 SGTAIRPIALRAVTAIARALPGFPILATGGIDSAESGLQFLHSGASVLQVCSAIQNQDFT 831

Query: 348 LVKKLCAELKDFMKLHNFSSIEEFRGASLP 359
           +++  C  LK  + L +   + ++ G S P
Sbjct: 832 VIEDYCTGLKALLYLKSIEELADWDGQSPP 855

[Close]
BLAST of CmaCh13G009770 vs. ExPASy TrEMBL
Match: A0A6J1KNM9 (Dihydrothymine dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111495821 PE=3 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 1.8e-229
Identity = 405/424 (95.52%), Postives = 405/424 (95.52%), Query Frame = 0 

Query: 1   MASLDFTQIRTRNPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN 60
           MASLDFTQIRTRNPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN
Sbjct: 1   MASLDFTQIRTRNPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN 60

Query: 61  PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSSGSAKGQ 120
           PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSSGSAKGQ
Sbjct: 61  PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSSGSAKGQ 120

Query: 121 IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD 180
           IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD
Sbjct: 121 IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD 180

Query: 181 AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV 240
           AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV
Sbjct: 181 AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV 240

Query: 241 ALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM 300
           ALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM
Sbjct: 241 ALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM 300

Query: 301 MKAEFNDADYSLSGIGG-------------------VCTGVMMHGYGLVKKLCAELKDFM 360
           MKAEFNDADYSLSGIGG                   VCTGVMMHGYGLVKKLCAELKDFM
Sbjct: 301 MKAEFNDADYSLSGIGGVETGGDAAEFILLGANTVQVCTGVMMHGYGLVKKLCAELKDFM 360

Query: 361 KLHNFSSIEEFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES 406
           KLHNFSSIEEFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES
Sbjct: 361 KLHNFSSIEEFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES 420

[Close]
BLAST of CmaCh13G009770 vs. ExPASy TrEMBL
Match: A0A6J1E7E7 (Dihydrothymine dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111431318 PE=3 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 6.4e-227
Identity = 400/424 (94.34%), Postives = 402/424 (94.81%), Query Frame = 0 

Query: 1   MASLDFTQIRTRNPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN 60
           MASLDFTQIRT NPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN
Sbjct: 1   MASLDFTQIRTTNPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN 60

Query: 61  PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSSGSAKGQ 120
           PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADS+GSAKGQ
Sbjct: 61  PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSNGSAKGQ 120

Query: 121 IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD 180
           IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD
Sbjct: 121 IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD 180

Query: 181 AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV 240
           AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV
Sbjct: 181 AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV 240

Query: 241 ALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM 300
           ALK GCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM
Sbjct: 241 ALKSGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM 300

Query: 301 MKAEFNDADYSLSGIGG-------------------VCTGVMMHGYGLVKKLCAELKDFM 360
           MKAEFND DYSLSGIGG                   VCTGVMMHGYGLVKKLCAELKDFM
Sbjct: 301 MKAEFNDGDYSLSGIGGVETGGDAAEFILLGANTVQVCTGVMMHGYGLVKKLCAELKDFM 360

Query: 361 KLHNFSSIEEFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES 406
           KLHNFSSIE+FRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES
Sbjct: 361 KLHNFSSIEDFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES 420

[Close]
BLAST of CmaCh13G009770 vs. ExPASy TrEMBL
Match: A0A6J1GUT8 (Dihydrothymine dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111457127 PE=3 SV=1)

HSP 1 Score: 763.1 bits (1969), Expect = 6.0e-217
Identity = 382/424 (90.09%), Postives = 392/424 (92.45%), Query Frame = 0 

Query: 1   MASLDFTQIRTRNPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN 60
           MASL+FTQIR +NP+S+L L RS+  L RR RVGF+VVAAGE   EPDLSVTVNGLHMPN
Sbjct: 1   MASLNFTQIRNKNPISDLTLTRSQTGLARRTRVGFKVVAAGESPVEPDLSVTVNGLHMPN 60

Query: 61  PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSSGSAKGQ 120
           PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADS+GS KGQ
Sbjct: 61  PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSNGSGKGQ 120

Query: 121 IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD 180
           IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD
Sbjct: 121 IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD 180

Query: 181 AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV 240
           AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDI+QPARV
Sbjct: 181 AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDIAQPARV 240

Query: 241 ALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM 300
           ALK GCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVL+IAKM
Sbjct: 241 ALKSGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLSIAKM 300

Query: 301 MKAEFNDADYSLSGIGG-------------------VCTGVMMHGYGLVKKLCAELKDFM 360
           MKAEFNDADYSLSGIGG                   VCTGVMMHGYGLVKKLCAELKD M
Sbjct: 301 MKAEFNDADYSLSGIGGVETGGDAAEFILLGANSVQVCTGVMMHGYGLVKKLCAELKDVM 360

Query: 361 KLHNFSSIEEFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES 406
           KLHNFSSIE+FRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES
Sbjct: 361 KLHNFSSIEDFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES 420

[Close]
BLAST of CmaCh13G009770 vs. ExPASy TrEMBL
Match: A0A6J1K219 (Dihydrothymine dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111489957 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 8.7e-216
Identity = 383/425 (90.12%), Postives = 392/425 (92.24%), Query Frame = 0 

Query: 1   MASLDFTQIRTRNPMSELALNRSRMR-LTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMP 60
           MASL+FTQIR +NP+SEL L RS+   L RR RVGF+VVAAGE   EPDLSVTVNGLHMP
Sbjct: 1   MASLNFTQIRNKNPISELTLTRSQTTGLARRTRVGFKVVAAGESPVEPDLSVTVNGLHMP 60

Query: 61  NPFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSSGSAKG 120
           NPFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADS+GSAKG
Sbjct: 61  NPFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSNGSAKG 120

Query: 121 QIIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGV 180
           QIIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNK AWEELIDRVEQTGV
Sbjct: 121 QIIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKDAWEELIDRVEQTGV 180

Query: 181 DAFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPAR 240
           DAFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDI+QPAR
Sbjct: 181 DAFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDIAQPAR 240

Query: 241 VALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAK 300
           VALK GCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVL+IAK
Sbjct: 241 VALKSGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLSIAK 300

Query: 301 MMKAEFNDADYSLSGIGG-------------------VCTGVMMHGYGLVKKLCAELKDF 360
           MMKAEFNDA YSLSGIGG                   VCTGVMMHGYGLVKKLCAELKDF
Sbjct: 301 MMKAEFNDAGYSLSGIGGVETGGDAAEFILLGANSVQVCTGVMMHGYGLVKKLCAELKDF 360

Query: 361 MKLHNFSSIEEFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETE 406
           MKLHNFSSIE+FRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETE
Sbjct: 361 MKLHNFSSIEDFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETE 420

[Close]
BLAST of CmaCh13G009770 vs. ExPASy TrEMBL
Match: A0A1S4DTL5 (Dihydrothymine dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103504066 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 1.9e-215
Identity = 381/424 (89.86%), Postives = 391/424 (92.22%), Query Frame = 0 

Query: 1   MASLDFTQIRTRNPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN 60
           MAS + TQ RT+NP+  LALN SR  LTR  R+GFRVVA+GE QAEPDLSVTVNGL+MPN
Sbjct: 1   MASFNLTQFRTKNPIPNLALNPSRTGLTRPTRLGFRVVASGEPQAEPDLSVTVNGLNMPN 60

Query: 61  PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSSGSAKGQ 120
           PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADS+GSAKGQ
Sbjct: 61  PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSNGSAKGQ 120

Query: 121 IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD 180
           IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD
Sbjct: 121 IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD 180

Query: 181 AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV 240
           AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV
Sbjct: 181 AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV 240

Query: 241 ALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM 300
           ALK GCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVL+IAKM
Sbjct: 241 ALKSGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLSIAKM 300

Query: 301 MKAEFNDADYSLSGIGG-------------------VCTGVMMHGYGLVKKLCAELKDFM 360
           MKAEFND DYSLSGIGG                   VCTGVMMHGYGLVKKLCAEL+DFM
Sbjct: 301 MKAEFNDGDYSLSGIGGVEAGGDAAEFILLGANTVQVCTGVMMHGYGLVKKLCAELQDFM 360

Query: 361 KLHNFSSIEEFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES 406
           KLHNFSSIE+FRGASLPYFTTH DLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES
Sbjct: 361 KLHNFSSIEDFRGASLPYFTTHMDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES 420

[Close]
BLAST of CmaCh13G009770 vs. NCBI nr
Match: XP_023001784.1 (dihydropyrimidine dehydrogenase (NADP(+)), chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 804.7 bits (2077), Expect = 3.7e-229
Identity = 405/424 (95.52%), Postives = 405/424 (95.52%), Query Frame = 0 

Query: 1   MASLDFTQIRTRNPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN 60
           MASLDFTQIRTRNPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN
Sbjct: 1   MASLDFTQIRTRNPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN 60

Query: 61  PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSSGSAKGQ 120
           PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSSGSAKGQ
Sbjct: 61  PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSSGSAKGQ 120

Query: 121 IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD 180
           IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD
Sbjct: 121 IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD 180

Query: 181 AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV 240
           AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV
Sbjct: 181 AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV 240

Query: 241 ALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM 300
           ALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM
Sbjct: 241 ALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM 300

Query: 301 MKAEFNDADYSLSGIGG-------------------VCTGVMMHGYGLVKKLCAELKDFM 360
           MKAEFNDADYSLSGIGG                   VCTGVMMHGYGLVKKLCAELKDFM
Sbjct: 301 MKAEFNDADYSLSGIGGVETGGDAAEFILLGANTVQVCTGVMMHGYGLVKKLCAELKDFM 360

Query: 361 KLHNFSSIEEFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES 406
           KLHNFSSIEEFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES
Sbjct: 361 KLHNFSSIEEFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES 420

[Close]
BLAST of CmaCh13G009770 vs. NCBI nr
Match: XP_023519817.1 (dihydropyrimidine dehydrogenase (NADP(+)), chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 797.7 bits (2059), Expect = 4.5e-227
Identity = 401/424 (94.58%), Postives = 403/424 (95.05%), Query Frame = 0 

Query: 1   MASLDFTQIRTRNPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN 60
           MASLDFTQIRTRNPMSELALNRSRMRL RRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN
Sbjct: 1   MASLDFTQIRTRNPMSELALNRSRMRLPRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN 60

Query: 61  PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSSGSAKGQ 120
           PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADS+GSAKGQ
Sbjct: 61  PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSNGSAKGQ 120

Query: 121 IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD 180
           IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD
Sbjct: 121 IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD 180

Query: 181 AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV 240
           AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV
Sbjct: 181 AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV 240

Query: 241 ALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM 300
           ALK GCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM
Sbjct: 241 ALKSGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM 300

Query: 301 MKAEFNDADYSLSGIGG-------------------VCTGVMMHGYGLVKKLCAELKDFM 360
           MKAEFNDADYSLSGIGG                   VCTGVMMHGYGLVKKLCAELKDFM
Sbjct: 301 MKAEFNDADYSLSGIGGVETGGDAAEFILLGANTVQVCTGVMMHGYGLVKKLCAELKDFM 360

Query: 361 KLHNFSSIEEFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES 406
           KLHNFSSIE+FRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES
Sbjct: 361 KLHNFSSIEDFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES 420

[Close]
BLAST of CmaCh13G009770 vs. NCBI nr
Match: XP_022923676.1 (dihydropyrimidine dehydrogenase (NADP(+)), chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 796.2 bits (2055), Expect = 1.3e-226
Identity = 400/424 (94.34%), Postives = 402/424 (94.81%), Query Frame = 0 

Query: 1   MASLDFTQIRTRNPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN 60
           MASLDFTQIRT NPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN
Sbjct: 1   MASLDFTQIRTTNPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN 60

Query: 61  PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSSGSAKGQ 120
           PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADS+GSAKGQ
Sbjct: 61  PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSNGSAKGQ 120

Query: 121 IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD 180
           IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD
Sbjct: 121 IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD 180

Query: 181 AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV 240
           AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV
Sbjct: 181 AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV 240

Query: 241 ALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM 300
           ALK GCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM
Sbjct: 241 ALKSGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM 300

Query: 301 MKAEFNDADYSLSGIGG-------------------VCTGVMMHGYGLVKKLCAELKDFM 360
           MKAEFND DYSLSGIGG                   VCTGVMMHGYGLVKKLCAELKDFM
Sbjct: 301 MKAEFNDGDYSLSGIGGVETGGDAAEFILLGANTVQVCTGVMMHGYGLVKKLCAELKDFM 360

Query: 361 KLHNFSSIEEFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES 406
           KLHNFSSIE+FRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES
Sbjct: 361 KLHNFSSIEDFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES 420

[Close]
BLAST of CmaCh13G009770 vs. NCBI nr
Match: KAG6584305.1 (Dihydropyrimidine dehydrogenase (NADP(+)), chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019899.1 Dihydropyrimidine dehydrogenase (NADP(+)), chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 793.9 bits (2049), Expect = 6.6e-226
Identity = 399/424 (94.10%), Postives = 402/424 (94.81%), Query Frame = 0 

Query: 1   MASLDFTQIRTRNPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN 60
           MASL FTQIRT NPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN
Sbjct: 1   MASLGFTQIRTTNPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN 60

Query: 61  PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSSGSAKGQ 120
           PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADS+GSAKGQ
Sbjct: 61  PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSNGSAKGQ 120

Query: 121 IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD 180
           IIGWENIELISDRPLETML+EFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD
Sbjct: 121 IIGWENIELISDRPLETMLREFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD 180

Query: 181 AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV 240
           AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV
Sbjct: 181 AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV 240

Query: 241 ALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM 300
           ALK GCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM
Sbjct: 241 ALKSGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM 300

Query: 301 MKAEFNDADYSLSGIGG-------------------VCTGVMMHGYGLVKKLCAELKDFM 360
           MKAEFNDADYSLSGIGG                   VCTGVMMHGYGLVKKLCAELKDFM
Sbjct: 301 MKAEFNDADYSLSGIGGVETGGDAAEFILLGANTVQVCTGVMMHGYGLVKKLCAELKDFM 360

Query: 361 KLHNFSSIEEFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES 406
           KLHNFSSIE+FRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES
Sbjct: 361 KLHNFSSIEDFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES 420

[Close]
BLAST of CmaCh13G009770 vs. NCBI nr
Match: KAG6572978.1 (Dihydropyrimidine dehydrogenase (NADP(+)), chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 769.6 bits (1986), Expect = 1.3e-218
Identity = 385/424 (90.80%), Postives = 395/424 (93.16%), Query Frame = 0 

Query: 1   MASLDFTQIRTRNPMSELALNRSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNGLHMPN 60
           MASL+FTQIR +NP+S+L L RS+M L RR RVGF+VVAAGE   EPDLSVTVNGLHMPN
Sbjct: 1   MASLNFTQIRNKNPISDLTLTRSQMGLARRTRVGFKVVAAGESPVEPDLSVTVNGLHMPN 60

Query: 61  PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSSGSAKGQ 120
           PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADS+GSAKGQ
Sbjct: 61  PFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSNGSAKGQ 120

Query: 121 IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD 180
           IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD
Sbjct: 121 IIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVEQTGVD 180

Query: 181 AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDISQPARV 240
           AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDI+QPARV
Sbjct: 181 AFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDIAQPARV 240

Query: 241 ALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLNIAKM 300
           ALK GCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVL+IAKM
Sbjct: 241 ALKSGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVLSIAKM 300

Query: 301 MKAEFNDADYSLSGIGG-------------------VCTGVMMHGYGLVKKLCAELKDFM 360
           MKAEFNDADYSLSGIGG                   VCTGVMMHGYGLVKKLCAELKDFM
Sbjct: 301 MKAEFNDADYSLSGIGGVETGGDAAEFILLGANSVQVCTGVMMHGYGLVKKLCAELKDFM 360

Query: 361 KLHNFSSIEEFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES 406
           KLHNFSSIE+FRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES
Sbjct: 361 KLHNFSSIEDFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFVKETES 420

[Close]
BLAST of CmaCh13G009770 vs. TAIR 10
Match: AT3G17810.1 (pyrimidine 1 )

HSP 1 Score: 651.4 bits (1679), Expect = 4.9e-187
Identity = 331/429 (77.16%), Postives = 362/429 (84.38%), Query Frame = 0 

Query: 1   MASLDFTQIRTRNPMSELALN-----RSRMRLTRRNRVGFRVVAAGEGQAEPDLSVTVNG 60
           MAS+ F   R     S+  L+      SR       RVG ++ +A E  +EPDLSVTVNG
Sbjct: 1   MASMSFALNRFSGLSSKTTLSADFDPSSRRSFLPPTRVGLKISSAAE--SEPDLSVTVNG 60

Query: 61  LHMPNPFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDAAKVINVTPRYARLRADSSG 120
           L MPNPFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDA+KVINVTPRYARLR  S+G
Sbjct: 61  LKMPNPFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDASKVINVTPRYARLRTGSNG 120

Query: 121 SAKGQIIGWENIELISDRPLETMLKEFKQLKEEYPDRILIASIMEEYNKAAWEELIDRVE 180
           SAK  +IGW+NIELISDRPLETMLKEF++LK+EYPDRILIAS+MEEYNK AWEELIDRVE
Sbjct: 121 SAKTDVIGWQNIELISDRPLETMLKEFERLKKEYPDRILIASVMEEYNKTAWEELIDRVE 180

Query: 181 QTGVDAFEINFSCPHGMPERRMGAAVGQDCALLEEVCGWINAKATIPVWAKMTPNITDIS 240
           QTGVDA EINFSCPHGMPERRMGAAVGQDCALL+EVCGWINAKAT+PVWAKMTPNITDI+
Sbjct: 181 QTGVDALEINFSCPHGMPERRMGAAVGQDCALLDEVCGWINAKATVPVWAKMTPNITDIT 240

Query: 241 QPARVALKLGCEGIAAINTIMSVMGIDLKTLRPEPCVEGYSTPGGYSSKAVHPIALGKVL 300
           +PARV+LK GCEGIAAINTIMSVMGID+KTLRPEPCVEGYSTPGGYS KAV PIAL KV+
Sbjct: 241 EPARVSLKSGCEGIAAINTIMSVMGIDMKTLRPEPCVEGYSTPGGYSYKAVRPIALAKVM 300

Query: 301 NIAKMMKAEFNDADYSLSGIGG-------------------VCTGVMMHGYGLVKKLCAE 360
           NIAKMMK+EF++ D SLSGIGG                   VCTGVMMHGYG VK LCAE
Sbjct: 301 NIAKMMKSEFSE-DRSLSGIGGVETGYDAAEFILLGSNTVQVCTGVMMHGYGHVKTLCAE 360

Query: 361 LKDFMKLHNFSSIEEFRGASLPYFTTHTDLVRRQREAIEQRKAVKKGLQSDKDWTGDGFV 406
           LKDFMK HNFS+IEEFRG SL YFTTHTDLV+RQ+EA+EQRKA K+GL+SDKDWTGDGFV
Sbjct: 361 LKDFMKQHNFSTIEEFRGHSLQYFTTHTDLVKRQKEAVEQRKAEKRGLKSDKDWTGDGFV 420

The following BLAST results are available for this feature:
BLAST of CmaCh13G009770 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LVI96.9e-18677.16Dihydropyrimidine dehydrogenase (NADP(+)), chloroplastic OS=Arabidopsis thaliana... [more]
Q8X6435.5e-5838.82NAD-dependent dihydropyrimidine dehydrogenase subunit PreA OS=Escherichia coli O... [more]
P258895.5e-5838.82NAD-dependent dihydropyrimidine dehydrogenase subunit PreA OS=Escherichia coli (... [more]
Q8ZNL72.3e-5638.34NAD-dependent dihydropyrimidine dehydrogenase subunit PreA OS=Salmonella typhimu... [more]
Q8CHR69.7e-5536.97Dihydropyrimidine dehydrogenase [NADP(+)] OS=Mus musculus OX=10090 GN=Dpyd PE=1 ... [more]
BLAST of CmaCh13G009770 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1KNM91.8e-22995.52Dihydrothymine dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111495821 PE=3 SV... [more]
A0A6J1E7E76.4e-22794.34Dihydrothymine dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111431318 PE=3 ... [more]
A0A6J1GUT86.0e-21790.09Dihydrothymine dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111457127 PE=3 ... [more]
A0A6J1K2198.7e-21690.12Dihydrothymine dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111489957 PE=3 SV... [more]
A0A1S4DTL51.9e-21589.86Dihydrothymine dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103504066 PE=3 SV=1[more]
BLAST of CmaCh13G009770 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023001784.13.7e-22995.52dihydropyrimidine dehydrogenase (NADP(+)), chloroplastic-like [Cucurbita maxima][more]
XP_023519817.14.5e-22794.58dihydropyrimidine dehydrogenase (NADP(+)), chloroplastic-like [Cucurbita pepo su... [more]
XP_022923676.11.3e-22694.34dihydropyrimidine dehydrogenase (NADP(+)), chloroplastic-like [Cucurbita moschat... [more]
KAG6584305.16.6e-22694.10Dihydropyrimidine dehydrogenase (NADP(+)), chloroplastic, partial [Cucurbita arg... [more]
KAG6572978.11.3e-21890.80Dihydropyrimidine dehydrogenase (NADP(+)), chloroplastic, partial [Cucurbita arg... [more]
BLAST of CmaCh13G009770 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G17810.14.9e-18777.16pyrimidine 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005720Dihydroorotate dehydrogenase domainPFAMPF01180DHO_dhcoord: 49..321
e-value: 2.9E-21
score: 76.0
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 49..346
e-value: 1.0E-81
score: 276.8
IPR044512Dihydropyrimidine dehydrogenasePANTHERPTHR43073DIHYDROPYRIMIDINE DEHYDROGENASE [NADP(+)]coord: 28..405
NoneNo IPR availablePANTHERPTHR43073:SF3BNAA01G27800D PROTEINcoord: 28..405
NoneNo IPR availableCDDcd02940DHPD_FMNcoord: 48..337
e-value: 5.55568E-162
score: 455.205
NoneNo IPR availableSUPERFAMILY51395FMN-linked oxidoreductasescoord: 43..357

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh13G009770.1CmaCh13G009770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019483 beta-alanine biosynthetic process
biological_process GO:0006210 thymine catabolic process
biological_process GO:0006212 uracil catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0017113 dihydropyrimidine dehydrogenase (NADP+) activity
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0050661 NADP binding
molecular_function GO:0002058 uracil binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors