CmaCh13G009500 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh13G009500
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptiontonoplast intrinsic protein 2
LocationCma_Chr13: 7449215 .. 7450530 (-)
RNA-Seq ExpressionCmaCh13G009500
SyntenyCmaCh13G009500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGCCGAGACGATATGCTTTTGGACGGGCAGATGAGGCCACCCACCCTGACTCCATTAGAGCCACCTTGGCTGAGTTCATTTCCACTTTCATCTTCGTCTTCGCCGGCGAAGGCTCTGTTCTTGCTCTTGGTATGTTGTTCTAAAAAATTCTTCATTTAAATTCTTACCCAAATGCATGCATGAATTAATTGATTAATCCTTCGATTTTGCAGACAAAATTTTCAGTCCAGCAGATTATGGAAGTTATGGCCATGGAGGTTACAGTCACGGTGACGGTTATGGTCGGAGGAGGACCGACACGGGGCGAGGTGCATCCGATTTAGTTGTGATAGCGATAGCGCACGCATTCGCTCTGTTCTCAGCGGTTGCGGCGAGTATCAACATCTCCGGTGGGCACGTCAATCCGGCTGTGACTTTTGGCGCTCTTCTTGGCGGGAGGATATCACTTATCCGTGGAATTTTCTATTGGGTAGCTCAAATTTTGGGTGCCATCGTCGCTTCACTGCTCTTGAGACTCGCAACCGGTGGCATGGTAAAGAAAGGAAATGATTTGTTTATGTTGTTGTTTTTTTTTTTAAATGGATGAATAATTTTATAATTGGGAAATTGCAGAGGCCTATGGGGTTCTTCGTTTCATCAGGGATATCAGAAATGCACGGCTTTCTGCTAGAGATAGTGCTGACGTTCGCCTTGGTGTACACAGTCTACGCAACAGCAATAGACCCAAAGAGAGGCAGTTTGGGGACTATAGCGCCACTGGCAATCGGGTTAATAGTGGGGGCTAATATTCTGGTGGGTGGGGCGTTTGATGGGGCGTGCATGAACCCGGCGAGAGCGTTTGGGCCGTCGTTGGTGGGGTGGAGATGGGACAATCATTGGATCTACTGGATTGGGCCATTGCTGGGAGGTGGGCTTGCTGCGCTTGTTTATGAGTACTTGGTGATTCCCGTTGAGCCTCCTCTGCATACTCACCAGCCCTTAGCTCCGGAGGATTACTAAGCTTTTGGTTTTTGAATTTGTAGAGACAGATTCGAGTGTGTGGTTTGGATGTGAGACTGTTGTTTTTTTGTAGTCTATTTTGTTTGTTTGTTGTCTCCGTTGGGGTTATTTGTGGTGTTTATGTCGTTCATCAAGGTAATAAAAGCAATACAGCTTACATCTTTTTCTGGTGTTCTTTGTTCTGTTTGGTTTGAAAATTAGAAGGTGGGTTCATGGTTTTTGGCGCTTAATCCTTATCCTTTTCGACCTTGGGCTCATTTTTGTCGATTATTACCTTGATGATAACGAAACGTGGGATATGGGTATGCCGTAT

mRNA sequence

ATGCCGCCGAGACGATATGCTTTTGGACGGGCAGATGAGGCCACCCACCCTGACTCCATTAGAGCCACCTTGGCTGAGTTCATTTCCACTTTCATCTTCGTCTTCGCCGGCGAAGGCTCTGTTCTTGCTCTTGACAAAATTTTCAGTCCAGCAGATTATGGAAGTTATGGCCATGGAGGTTACAGTCACGGTGACGGTTATGGTCGGAGGAGGACCGACACGGGGCGAGGTGCATCCGATTTAGTTGTGATAGCGATAGCGCACGCATTCGCTCTGTTCTCAGCGGTTGCGGCGAGTATCAACATCTCCGGTGGGCACGTCAATCCGGCTGTGACTTTTGGCGCTCTTCTTGGCGGGAGGATATCACTTATCCGTGGAATTTTCTATTGGGTAGCTCAAATTTTGGGTGCCATCGTCGCTTCACTGCTCTTGAGACTCGCAACCGGTGGCATGAGGCCTATGGGGTTCTTCGTTTCATCAGGGATATCAGAAATGCACGGCTTTCTGCTAGAGATAGTGCTGACGTTCGCCTTGGTGTACACAGTCTACGCAACAGCAATAGACCCAAAGAGAGGCAGTTTGGGGACTATAGCGCCACTGGCAATCGGGTTAATAGTGGGGGCTAATATTCTGGTGGGTGGGGCGTTTGATGGGGCGTGCATGAACCCGGCGAGAGCGTTTGGGCCGTCGTTGGTGGGGTGGAGATGGGACAATCATTGGATCTACTGGATTGGGCCATTGCTGGGAGGTGGGCTTGCTGCGCTTGTTTATGAGTACTTGGTGATTCCCGTTGAGCCTCCTCTGCATACTCACCAGCCCTTAGCTCCGGAGGATTACTAAGCTTTTGGTTTTTGAATTTGTAGAGACAGATTCGAGTGTGTGGTTTGGATGTGAGACTGTTGTTTTTTTGTAGTCTATTTTGTTTGTTTGTTGTCTCCGTTGGGGTTATTTGTGGTGTTTATGTCGTTCATCAAGGTAATAAAAGCAATACAGCTTACATCTTTTTCTGGTGTTCTTTGTTCTGTTTGGTTTGAAAATTAGAAGGTGGGTTCATGGTTTTTGGCGCTTAATCCTTATCCTTTTCGACCTTGGGCTCATTTTTGTCGATTATTACCTTGATGATAACGAAACGTGGGATATGGGTATGCCGTAT

Coding sequence (CDS)

ATGCCGCCGAGACGATATGCTTTTGGACGGGCAGATGAGGCCACCCACCCTGACTCCATTAGAGCCACCTTGGCTGAGTTCATTTCCACTTTCATCTTCGTCTTCGCCGGCGAAGGCTCTGTTCTTGCTCTTGACAAAATTTTCAGTCCAGCAGATTATGGAAGTTATGGCCATGGAGGTTACAGTCACGGTGACGGTTATGGTCGGAGGAGGACCGACACGGGGCGAGGTGCATCCGATTTAGTTGTGATAGCGATAGCGCACGCATTCGCTCTGTTCTCAGCGGTTGCGGCGAGTATCAACATCTCCGGTGGGCACGTCAATCCGGCTGTGACTTTTGGCGCTCTTCTTGGCGGGAGGATATCACTTATCCGTGGAATTTTCTATTGGGTAGCTCAAATTTTGGGTGCCATCGTCGCTTCACTGCTCTTGAGACTCGCAACCGGTGGCATGAGGCCTATGGGGTTCTTCGTTTCATCAGGGATATCAGAAATGCACGGCTTTCTGCTAGAGATAGTGCTGACGTTCGCCTTGGTGTACACAGTCTACGCAACAGCAATAGACCCAAAGAGAGGCAGTTTGGGGACTATAGCGCCACTGGCAATCGGGTTAATAGTGGGGGCTAATATTCTGGTGGGTGGGGCGTTTGATGGGGCGTGCATGAACCCGGCGAGAGCGTTTGGGCCGTCGTTGGTGGGGTGGAGATGGGACAATCATTGGATCTACTGGATTGGGCCATTGCTGGGAGGTGGGCTTGCTGCGCTTGTTTATGAGTACTTGGTGATTCCCGTTGAGCCTCCTCTGCATACTCACCAGCCCTTAGCTCCGGAGGATTACTAA

Protein sequence

MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGGYSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGRISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Homology
BLAST of CmaCh13G009500 vs. ExPASy Swiss-Prot
Match: O22588 (Probable aquaporin TIP3-2 OS=Arabidopsis thaliana OX=3702 GN=TIP3-2 PE=2 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 3.4e-107
Identity = 198/279 (70.97%), Postives = 234/279 (83.87%), Query Frame = 0

Query: 4   RRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGGYSH 63
           R Y FGRADEATHPDSIRATLAEF+STF+FVFAGEGS+LALDK++    + +  H G   
Sbjct: 7   RAYGFGRADEATHPDSIRATLAEFLSTFVFVFAGEGSILALDKLY----WDTAAHTG--- 66

Query: 64  GDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGRISL 123
                   T+T  G   LV++A+AHA ALF+AV+A+IN+SGGHVNPAVTF AL+GGRIS+
Sbjct: 67  --------TNTPGG---LVLVALAHALALFAAVSAAINVSGGHVNPAVTFAALIGGRISV 126

Query: 124 IRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVYTVY 183
           IR I+YWVAQ++GAI+A LLLRLAT G+RP+GF V+SG+SE+HG L+EI+LTFALVY VY
Sbjct: 127 IRAIYYWVAQLIGAILACLLLRLATNGLRPVGFHVASGVSELHGLLMEIILTFALVYVVY 186

Query: 184 ATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHWIYW 243
           +TAIDPKRGS+G IAPLAIGLIVGANILVGG FDGA MNPARAFGP+LVGWRW NHWIYW
Sbjct: 187 STAIDPKRGSIGIIAPLAIGLIVGANILVGGPFDGASMNPARAFGPALVGWRWSNHWIYW 246

Query: 244 IGPLLGGGLAALVYEYLVIPV--EPPLH-THQPLAPEDY 280
           +GP +GG LAAL+YEY++IP   EPP H THQPLAPEDY
Sbjct: 247 VGPFIGGALAALIYEYMIIPSVNEPPHHSTHQPLAPEDY 267

BLAST of CmaCh13G009500 vs. ExPASy Swiss-Prot
Match: P26587 (Aquaporin TIP3-1 OS=Arabidopsis thaliana OX=3702 GN=TIP3-1 PE=2 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 5.6e-102
Identity = 187/280 (66.79%), Postives = 225/280 (80.36%), Query Frame = 0

Query: 4   RRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGGYSH 63
           R Y FGRADEATHPDSIRATLAEF+STF+FVFA EGS+L+LDK++    +    H G   
Sbjct: 7   RAYGFGRADEATHPDSIRATLAEFLSTFVFVFAAEGSILSLDKLY----WEHAAHAG--- 66

Query: 64  GDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGRISL 123
                   T+T  G   L+++A+AHAFALF+AV+A+IN+SGGHVNPAVTFGAL+GGR++ 
Sbjct: 67  --------TNTPGG---LILVALAHAFALFAAVSAAINVSGGHVNPAVTFGALVGGRVTA 126

Query: 124 IRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVYTVY 183
           IR I+YW+AQ+LGAI+A LLLRL T GMRP+GF ++SG+  ++G +LEI+LTF LVY VY
Sbjct: 127 IRAIYYWIAQLLGAILACLLLRLTTNGMRPVGFRLASGVGAVNGLVLEIILTFGLVYVVY 186

Query: 184 ATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHWIYW 243
           +T IDPKRGSLG IAPLAIGLIVGANILVGG F GA MNPARAFGP+LVGWRW +HWIYW
Sbjct: 187 STLIDPKRGSLGIIAPLAIGLIVGANILVGGPFSGASMNPARAFGPALVGWRWHDHWIYW 246

Query: 244 IGPLLGGGLAALVYEYLVIPVEPPLH----THQPLAPEDY 280
           +GP +G  LAAL+YEY+VIP EPP H     HQPLAPEDY
Sbjct: 247 VGPFIGSALAALIYEYMVIPTEPPTHHAHGVHQPLAPEDY 268

BLAST of CmaCh13G009500 vs. ExPASy Swiss-Prot
Match: P23958 (Probable aquaporin TIP-type alpha OS=Phaseolus vulgaris OX=3885 PE=1 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 3.6e-101
Identity = 184/279 (65.95%), Postives = 219/279 (78.49%), Query Frame = 0

Query: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60
           M  RRY+FGR DEATHPDS+RA+LAEF STFIFVFAGEGS LAL KI+            
Sbjct: 1   MATRRYSFGRTDEATHPDSMRASLAEFASTFIFVFAGEGSGLALVKIYQ----------- 60

Query: 61  YSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120
                       D+   A +L+ +A+AHAFALF+AV+AS+++SGGHVNPAV+FGAL+GGR
Sbjct: 61  ------------DSAFSAGELLALALAHAFALFAAVSASMHVSGGHVNPAVSFGALIGGR 120

Query: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180
           IS+IR ++YW+AQ+LG+IVA+L+LRL T  MRP GF VS G+   H F+LE+V+TF L+Y
Sbjct: 121 ISVIRAVYYWIAQLLGSIVAALVLRLVTNNMRPSGFHVSPGVGVGHMFILEVVMTFGLMY 180

Query: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240
           TVY TAIDPKRG++  IAPLAIGLIVGANILVGG FDGACMNPA AFGPSLVGW+W  HW
Sbjct: 181 TVYGTAIDPKRGAVSYIAPLAIGLIVGANILVGGPFDGACMNPALAFGPSLVGWQWHQHW 240

Query: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 280
           I+W+GPLLG  LAALVYEY VIP+EPP H HQPLA EDY
Sbjct: 241 IFWVGPLLGAALAALVYEYAVIPIEPPPHHHQPLATEDY 256

BLAST of CmaCh13G009500 vs. ExPASy Swiss-Prot
Match: Q9ATL7 (Aquaporin TIP3-1 OS=Zea mays OX=4577 GN=TIP3-1 PE=2 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.2e-91
Identity = 168/280 (60.00%), Postives = 205/280 (73.21%), Query Frame = 0

Query: 2   PPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGGY 61
           P RR+  GR+++ATHPD+IRA ++EFI+T IFVFA EGSVL+L K++             
Sbjct: 7   PGRRFTVGRSEDATHPDTIRAAISEFIATAIFVFAAEGSVLSLGKMYHDMS--------- 66

Query: 62  SHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGRI 121
                           A  LV +A+AHA AL  AVA ++NISGGHVNPAVTFGAL+GGR+
Sbjct: 67  ---------------TAGGLVAVALAHALALAVAVAVAVNISGGHVNPAVTFGALVGGRV 126

Query: 122 SLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVYT 181
           SL+R + YWVAQ+LGA+ A+LLLRLATGGMRP GF ++SG+ + H  LLE V+TF L+Y 
Sbjct: 127 SLVRAVLYWVAQLLGAVAATLLLRLATGGMRPPGFALASGVGDWHAVLLEAVMTFGLMYA 186

Query: 182 VYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHWI 241
            YAT IDPKRG +GTIAPLA+G ++GAN+L GG FDGA MNPAR FGP+LVGWRW +HW+
Sbjct: 187 YYATVIDPKRGHVGTIAPLAVGFLLGANVLAGGPFDGAGMNPARVFGPALVGWRWRHHWV 246

Query: 242 YWIGPLLGGGLAALVYEYLVIPVEPPL--HTHQPLAPEDY 280
           YW+GP LG GLA LVYEYLVIP       H HQPLAPEDY
Sbjct: 247 YWLGPFLGAGLAGLVYEYLVIPSADAAVPHAHQPLAPEDY 262

BLAST of CmaCh13G009500 vs. ExPASy Swiss-Prot
Match: Q9AT75 (Aquaporin TIP3-2 OS=Zea mays OX=4577 GN=TIP3-2 PE=2 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 6.4e-90
Identity = 165/277 (59.57%), Postives = 205/277 (74.01%), Query Frame = 0

Query: 4   RRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGGYSH 63
           RR+  GR+++ATHPD+IRA ++EFI+T IFVFA EGSVL+L K++       + H   S 
Sbjct: 11  RRFTVGRSEDATHPDTIRAAISEFIATAIFVFAAEGSVLSLGKMY-------HDHSTIST 70

Query: 64  GDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGRISL 123
             G              LV +A+AHA  L  AVA ++N+SGGHVNPAVTFGAL+GGR+SL
Sbjct: 71  AGG--------------LVAVALAHALGLAVAVAVAVNVSGGHVNPAVTFGALVGGRVSL 130

Query: 124 IRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVYTVY 183
           +R + YW AQ+LGA+ A+LLLRLATGG RP GF ++SG+ + H  LLE V+TF  VY  Y
Sbjct: 131 VRAVLYWAAQLLGAVAATLLLRLATGGARPPGFALASGVGDGHAVLLEAVMTFGFVYAYY 190

Query: 184 ATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHWIYW 243
           AT +DPKRG LGTIAPLA+G ++GAN+L GG FDGA MNPAR FGP+LVGWRW +HW+YW
Sbjct: 191 ATVVDPKRGHLGTIAPLAVGFLLGANVLAGGPFDGAGMNPARVFGPALVGWRWRHHWVYW 250

Query: 244 IGPLLGGGLAALVYEYLVI-PVEPPLHTHQPLAPEDY 280
           +GP LG GLA LVYEYL+I P +   HTHQPLAPEDY
Sbjct: 251 LGPFLGAGLAGLVYEYLLIPPADAVPHTHQPLAPEDY 266

BLAST of CmaCh13G009500 vs. ExPASy TrEMBL
Match: A0A6J1KM69 (probable aquaporin TIP3-2 OS=Cucurbita maxima OX=3661 GN=LOC111495835 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.2e-154
Identity = 279/279 (100.00%), Postives = 279/279 (100.00%), Query Frame = 0

Query: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60
           MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60

Query: 61  YSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120
           YSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR
Sbjct: 61  YSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120

Query: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180
           ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY
Sbjct: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180

Query: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240
           TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW
Sbjct: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240

Query: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 280
           IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 279

BLAST of CmaCh13G009500 vs. ExPASy TrEMBL
Match: Q39646 (MP23 OS=Cucurbita hybrid cultivar OX=170850 PE=2 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.2e-154
Identity = 279/279 (100.00%), Postives = 279/279 (100.00%), Query Frame = 0

Query: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60
           MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60

Query: 61  YSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120
           YSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR
Sbjct: 61  YSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120

Query: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180
           ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY
Sbjct: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180

Query: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240
           TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW
Sbjct: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240

Query: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 280
           IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 279

BLAST of CmaCh13G009500 vs. ExPASy TrEMBL
Match: A0A6J1E6R9 (probable aquaporin TIP3-2 OS=Cucurbita moschata OX=3662 GN=LOC111431313 PE=3 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 1.2e-152
Identity = 277/279 (99.28%), Postives = 277/279 (99.28%), Query Frame = 0

Query: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60
           MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60

Query: 61  YSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120
           YS G GYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR
Sbjct: 61  YSRGHGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120

Query: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180
           ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY
Sbjct: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180

Query: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240
           TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW
Sbjct: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240

Query: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 280
           IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 279

BLAST of CmaCh13G009500 vs. ExPASy TrEMBL
Match: A0A0A0LTX0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043290 PE=3 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.1e-145
Identity = 265/289 (91.70%), Postives = 273/289 (94.46%), Query Frame = 0

Query: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGH-- 60
           MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIF PADYGSYGH  
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFRPADYGSYGHGS 60

Query: 61  --------GGYSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPA 120
                   GGYS+G GYGR+ TDTGR ASDLVVIAIAHAFALFSAVAASINISGGHVNPA
Sbjct: 61  YGHGSYGRGGYSYGHGYGRKGTDTGRAASDLVVIAIAHAFALFSAVAASINISGGHVNPA 120

Query: 121 VTFGALLGGRISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLL 180
           VTFGAL+GGRISLIR  FYWVAQILGAI+ASLLLRLATGGMRPMGFFVSSG+SE+HGFLL
Sbjct: 121 VTFGALIGGRISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGVSELHGFLL 180

Query: 181 EIVLTFALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPS 240
           EI+LTFALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPS
Sbjct: 181 EIILTFALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPS 240

Query: 241 LVGWRWDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 280
           LVGWRWDNHWIYWIGPL+GGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 LVGWRWDNHWIYWIGPLIGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 289

BLAST of CmaCh13G009500 vs. ExPASy TrEMBL
Match: A0A1S3AU56 (probable aquaporin TIP3-2 OS=Cucumis melo OX=3656 GN=LOC103482730 PE=3 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 7.2e-145
Identity = 263/284 (92.61%), Postives = 271/284 (95.42%), Query Frame = 0

Query: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGH-- 60
           MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIF PADYGSYGH  
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFRPADYGSYGHGS 60

Query: 61  ---GGYSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGA 120
              GG+ +G GYGR+  DTGR ASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGA
Sbjct: 61  YGRGGHGYGHGYGRKGADTGRAASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGA 120

Query: 121 LLGGRISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLT 180
           L+GGRISLIR  FYWVAQILGAI+ASLLLRLATGGMRPMGFFVSSG+SE+HGFLLEI+LT
Sbjct: 121 LIGGRISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGVSELHGFLLEIILT 180

Query: 181 FALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWR 240
           FALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWR
Sbjct: 181 FALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWR 240

Query: 241 WDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 280
           WDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 WDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 284

BLAST of CmaCh13G009500 vs. NCBI nr
Match: XP_023001805.1 (probable aquaporin TIP3-2 [Cucurbita maxima] >pir|T10251| membrane protein MP23 precursor - cucurbit >BAA08107.1 MP23 precursor [Cucurbita hybrid cultivar])

HSP 1 Score: 554.7 bits (1428), Expect = 4.6e-154
Identity = 279/279 (100.00%), Postives = 279/279 (100.00%), Query Frame = 0

Query: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60
           MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60

Query: 61  YSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120
           YSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR
Sbjct: 61  YSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120

Query: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180
           ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY
Sbjct: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180

Query: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240
           TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW
Sbjct: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240

Query: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 280
           IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 279

BLAST of CmaCh13G009500 vs. NCBI nr
Match: XP_023519163.1 (probable aquaporin TIP3-2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 552.0 bits (1421), Expect = 3.0e-153
Identity = 278/279 (99.64%), Postives = 278/279 (99.64%), Query Frame = 0

Query: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60
           MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60

Query: 61  YSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120
           YSHG GYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR
Sbjct: 61  YSHGHGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120

Query: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180
           ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY
Sbjct: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180

Query: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240
           TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW
Sbjct: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240

Query: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 280
           IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 279

BLAST of CmaCh13G009500 vs. NCBI nr
Match: KAG6584278.1 (putative aquaporin TIP3-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 551.6 bits (1420), Expect = 3.9e-153
Identity = 277/279 (99.28%), Postives = 278/279 (99.64%), Query Frame = 0

Query: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60
           MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60

Query: 61  YSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120
           YSHG GYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR
Sbjct: 61  YSHGHGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120

Query: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180
           ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY
Sbjct: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180

Query: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240
           TVYATAIDPKRGSLGTIAPLAIGL+VGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW
Sbjct: 181 TVYATAIDPKRGSLGTIAPLAIGLVVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240

Query: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 280
           IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 279

BLAST of CmaCh13G009500 vs. NCBI nr
Match: KAG7019875.1 (putative aquaporin TIP3-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 550.8 bits (1418), Expect = 6.7e-153
Identity = 277/279 (99.28%), Postives = 278/279 (99.64%), Query Frame = 0

Query: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60
           MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60

Query: 61  YSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120
           YSHG GYGRR+TDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR
Sbjct: 61  YSHGHGYGRRKTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120

Query: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180
           ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY
Sbjct: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180

Query: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240
           TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW
Sbjct: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240

Query: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 280
           IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 279

BLAST of CmaCh13G009500 vs. NCBI nr
Match: XP_022923672.1 (probable aquaporin TIP3-2 [Cucurbita moschata])

HSP 1 Score: 548.9 bits (1413), Expect = 2.5e-152
Identity = 277/279 (99.28%), Postives = 277/279 (99.28%), Query Frame = 0

Query: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60
           MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60

Query: 61  YSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120
           YS G GYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR
Sbjct: 61  YSRGHGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120

Query: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180
           ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY
Sbjct: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180

Query: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240
           TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW
Sbjct: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240

Query: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 280
           IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 279

BLAST of CmaCh13G009500 vs. TAIR 10
Match: AT1G17810.1 (beta-tonoplast intrinsic protein )

HSP 1 Score: 389.4 bits (999), Expect = 2.4e-108
Identity = 198/279 (70.97%), Postives = 234/279 (83.87%), Query Frame = 0

Query: 4   RRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGGYSH 63
           R Y FGRADEATHPDSIRATLAEF+STF+FVFAGEGS+LALDK++    + +  H G   
Sbjct: 7   RAYGFGRADEATHPDSIRATLAEFLSTFVFVFAGEGSILALDKLY----WDTAAHTG--- 66

Query: 64  GDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGRISL 123
                   T+T  G   LV++A+AHA ALF+AV+A+IN+SGGHVNPAVTF AL+GGRIS+
Sbjct: 67  --------TNTPGG---LVLVALAHALALFAAVSAAINVSGGHVNPAVTFAALIGGRISV 126

Query: 124 IRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVYTVY 183
           IR I+YWVAQ++GAI+A LLLRLAT G+RP+GF V+SG+SE+HG L+EI+LTFALVY VY
Sbjct: 127 IRAIYYWVAQLIGAILACLLLRLATNGLRPVGFHVASGVSELHGLLMEIILTFALVYVVY 186

Query: 184 ATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHWIYW 243
           +TAIDPKRGS+G IAPLAIGLIVGANILVGG FDGA MNPARAFGP+LVGWRW NHWIYW
Sbjct: 187 STAIDPKRGSIGIIAPLAIGLIVGANILVGGPFDGASMNPARAFGPALVGWRWSNHWIYW 246

Query: 244 IGPLLGGGLAALVYEYLVIPV--EPPLH-THQPLAPEDY 280
           +GP +GG LAAL+YEY++IP   EPP H THQPLAPEDY
Sbjct: 247 VGPFIGGALAALIYEYMIIPSVNEPPHHSTHQPLAPEDY 267

BLAST of CmaCh13G009500 vs. TAIR 10
Match: AT1G73190.1 (Aquaporin-like superfamily protein )

HSP 1 Score: 372.1 bits (954), Expect = 4.0e-103
Identity = 187/280 (66.79%), Postives = 225/280 (80.36%), Query Frame = 0

Query: 4   RRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGGYSH 63
           R Y FGRADEATHPDSIRATLAEF+STF+FVFA EGS+L+LDK++    +    H G   
Sbjct: 7   RAYGFGRADEATHPDSIRATLAEFLSTFVFVFAAEGSILSLDKLY----WEHAAHAG--- 66

Query: 64  GDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGRISL 123
                   T+T  G   L+++A+AHAFALF+AV+A+IN+SGGHVNPAVTFGAL+GGR++ 
Sbjct: 67  --------TNTPGG---LILVALAHAFALFAAVSAAINVSGGHVNPAVTFGALVGGRVTA 126

Query: 124 IRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVYTVY 183
           IR I+YW+AQ+LGAI+A LLLRL T GMRP+GF ++SG+  ++G +LEI+LTF LVY VY
Sbjct: 127 IRAIYYWIAQLLGAILACLLLRLTTNGMRPVGFRLASGVGAVNGLVLEIILTFGLVYVVY 186

Query: 184 ATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHWIYW 243
           +T IDPKRGSLG IAPLAIGLIVGANILVGG F GA MNPARAFGP+LVGWRW +HWIYW
Sbjct: 187 STLIDPKRGSLGIIAPLAIGLIVGANILVGGPFSGASMNPARAFGPALVGWRWHDHWIYW 246

Query: 244 IGPLLGGGLAALVYEYLVIPVEPPLH----THQPLAPEDY 280
           +GP +G  LAAL+YEY+VIP EPP H     HQPLAPEDY
Sbjct: 247 VGPFIGSALAALIYEYMVIPTEPPTHHAHGVHQPLAPEDY 268

BLAST of CmaCh13G009500 vs. TAIR 10
Match: AT2G36830.1 (gamma tonoplast intrinsic protein )

HSP 1 Score: 300.8 bits (769), Expect = 1.1e-81
Identity = 157/280 (56.07%), Postives = 191/280 (68.21%), Query Frame = 0

Query: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60
           MP R  A GR DEAT PD+++A LAEFIST IFV AG GS +A +K+             
Sbjct: 1   MPIRNIAIGRPDEATRPDALKAALAEFISTLIFVVAGSGSGMAFNKL------------- 60

Query: 61  YSHGDGYGRRRTDTGRGA-SDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGG 120
                      T+ G    S LV  A+AHAF LF AV+   NISGGHVNPAVTFGA +GG
Sbjct: 61  -----------TENGATTPSGLVAAAVAHAFGLFVAVSVGANISGGHVNPAVTFGAFIGG 120

Query: 121 RISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALV 180
            I+L+RGI YW+AQ+LG++VA L+L+ ATGG+    F +S+G+  ++ F+ EIV+TF LV
Sbjct: 121 NITLLRGILYWIAQLLGSVVACLILKFATGGLAVPAFGLSAGVGVLNAFVFEIVMTFGLV 180

Query: 181 YTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNH 240
           YTVYATAIDPK GSLGTIAP+AIG IVGANIL GGAF GA MNPA AFGP++V W W NH
Sbjct: 181 YTVYATAIDPKNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWTWTNH 240

Query: 241 WIYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 280
           W+YW GPL+GGG+A L+YE   I       TH+ L   DY
Sbjct: 241 WVYWAGPLVGGGIAGLIYEVFFINT-----THEQLPTTDY 251

BLAST of CmaCh13G009500 vs. TAIR 10
Match: AT3G26520.1 (tonoplast intrinsic protein 2 )

HSP 1 Score: 288.5 bits (737), Expect = 5.7e-78
Identity = 153/281 (54.45%), Postives = 187/281 (66.55%), Query Frame = 0

Query: 1   MPPRRYAFGRA-DEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHG 60
           MP R  A G   +E  HP+++RA LAEFIST IFVFAG GS +A +KI            
Sbjct: 1   MPTRNIAIGGVQEEVYHPNALRAALAEFISTLIFVFAGSGSGIAFNKI------------ 60

Query: 61  GYSHGDGYGRRRTDTGRGA-SDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLG 120
                       TD G    S LV  A+AHAF LF AV+   NISGGHVNPAVTFG LLG
Sbjct: 61  ------------TDNGATTPSGLVAAALAHAFGLFVAVSVGANISGGHVNPAVTFGVLLG 120

Query: 121 GRISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFAL 180
           G I+L+RGI YW+AQ+LG++ A  LL  ATGG     F +S+G+  ++  + EIV+TF L
Sbjct: 121 GNITLLRGILYWIAQLLGSVAACFLLSFATGGEPIPAFGLSAGVGSLNALVFEIVMTFGL 180

Query: 181 VYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDN 240
           VYTVYATA+DPK GSLGTIAP+AIG IVGANIL GGAF GA MNPA AFGP++V W W N
Sbjct: 181 VYTVYATAVDPKNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWTWTN 240

Query: 241 HWIYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 280
           HW+YW GPL+GGGLA ++Y+++ I      + H+ L   DY
Sbjct: 241 HWVYWAGPLIGGGLAGIIYDFVFIDE----NAHEQLPTTDY 253

BLAST of CmaCh13G009500 vs. TAIR 10
Match: AT4G01470.1 (tonoplast intrinsic protein 1;3 )

HSP 1 Score: 283.5 bits (724), Expect = 1.8e-76
Identity = 146/279 (52.33%), Postives = 187/279 (67.03%), Query Frame = 0

Query: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60
           MP  R A G   EA+ PD+IRA  AEF S  IFVFAG+GS                    
Sbjct: 1   MPINRIAIGTPGEASRPDAIRAAFAEFFSMVIFVFAGQGS-------------------- 60

Query: 61  YSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120
              G  YG+   D     + LV  +++HAFALF AV+   N+SGGHVNPAVTFGA +GG 
Sbjct: 61  ---GMAYGKLTGDGPATPAGLVAASLSHAFALFVAVSVGANVSGGHVNPAVTFGAFIGGN 120

Query: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180
           I+L+R I YW+AQ+LGA+VA LLL+++TGGM    F +S G++  +  + EIV+TF LVY
Sbjct: 121 ITLLRAILYWIAQLLGAVVACLLLKVSTGGMETAAFSLSYGVTPWNAVVFEIVMTFGLVY 180

Query: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240
           TVYATA+DPK+G +G IAPLAIGLIVGANILVGGAFDGA MNPA +FGP++V W W NHW
Sbjct: 181 TVYATAVDPKKGDIGIIAPLAIGLIVGANILVGGAFDGASMNPAVSFGPAVVSWIWTNHW 240

Query: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 280
           +YW+GP +G  +AA+VY+ + I      + H+PL   D+
Sbjct: 241 VYWVGPFIGAAIAAIVYDTIFI----GSNGHEPLPSNDF 252

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O225883.4e-10770.97Probable aquaporin TIP3-2 OS=Arabidopsis thaliana OX=3702 GN=TIP3-2 PE=2 SV=1[more]
P265875.6e-10266.79Aquaporin TIP3-1 OS=Arabidopsis thaliana OX=3702 GN=TIP3-1 PE=2 SV=1[more]
P239583.6e-10165.95Probable aquaporin TIP-type alpha OS=Phaseolus vulgaris OX=3885 PE=1 SV=1[more]
Q9ATL71.2e-9160.00Aquaporin TIP3-1 OS=Zea mays OX=4577 GN=TIP3-1 PE=2 SV=1[more]
Q9AT756.4e-9059.57Aquaporin TIP3-2 OS=Zea mays OX=4577 GN=TIP3-2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KM692.2e-154100.00probable aquaporin TIP3-2 OS=Cucurbita maxima OX=3661 GN=LOC111495835 PE=3 SV=1[more]
Q396462.2e-154100.00MP23 OS=Cucurbita hybrid cultivar OX=170850 PE=2 SV=1[more]
A0A6J1E6R91.2e-15299.28probable aquaporin TIP3-2 OS=Cucurbita moschata OX=3662 GN=LOC111431313 PE=3 SV=... [more]
A0A0A0LTX01.1e-14591.70Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043290 PE=3 SV=1[more]
A0A1S3AU567.2e-14592.61probable aquaporin TIP3-2 OS=Cucumis melo OX=3656 GN=LOC103482730 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_023001805.14.6e-154100.00probable aquaporin TIP3-2 [Cucurbita maxima] >pir|T10251| membrane protein MP23 ... [more]
XP_023519163.13.0e-15399.64probable aquaporin TIP3-2 [Cucurbita pepo subsp. pepo][more]
KAG6584278.13.9e-15399.28putative aquaporin TIP3-2, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7019875.16.7e-15399.28putative aquaporin TIP3-2, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022923672.12.5e-15299.28probable aquaporin TIP3-2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G17810.12.4e-10870.97beta-tonoplast intrinsic protein [more]
AT1G73190.14.0e-10366.79Aquaporin-like superfamily protein [more]
AT2G36830.11.1e-8156.07gamma tonoplast intrinsic protein [more]
AT3G26520.15.7e-7854.45tonoplast intrinsic protein 2 [more]
AT4G01470.11.8e-7652.33tonoplast intrinsic protein 1;3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 125..144
score: 43.63
coord: 21..40
score: 45.46
coord: 170..188
score: 41.95
coord: 240..260
score: 44.9
coord: 203..225
score: 42.55
coord: 88..112
score: 47.56
IPR000425Major intrinsic proteinTIGRFAMTIGR00861TIGR00861coord: 25..257
e-value: 5.0E-57
score: 191.2
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 14..257
e-value: 7.0E-75
score: 251.6
IPR000425Major intrinsic proteinCDDcd00333MIPcoord: 21..260
e-value: 2.08632E-70
score: 214.81
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 5..265
e-value: 3.7E-77
score: 260.7
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 13..270
NoneNo IPR availablePANTHERPTHR45665:SF39SUBFAMILY NOT NAMEDcoord: 72..279
NoneNo IPR availablePANTHERPTHR45665:SF39SUBFAMILY NOT NAMEDcoord: 3..49
IPR034294Aquaporin transporterPANTHERPTHR45665AQUAPORIN-8coord: 72..279
IPR034294Aquaporin transporterPANTHERPTHR45665AQUAPORIN-8coord: 3..49
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 106..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh13G009500.1CmaCh13G009500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0006833 water transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015250 water channel activity
molecular_function GO:0015267 channel activity