CmaCh13G009420 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh13G009420
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Cma_Chr13: 7429056 .. 7430769 (+)
RNA-Seq Expression	CmaCh13G009420
Synteny	CmaCh13G009420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACAACAAAAACAGATGCCACTTTCAAAATTTGGGTTATAAAAAGGGTGTTTTAGTGCCACATTTTCCAAGCCAAAAACACAGACAACAAACACTTAACAGAGCTGAGTTTTAGTGTTCATCAATGGCGGGTGAGATTCCAGTTGTGAGATTTAGTTCACACAATGTGCAGAGCGGAGGGAGGAAGTGGGGTTCGACGAGGGAGAAGGTCCGGGAAGCACTTGAAGAATATGGGTGCTTTGTAGCGTTGTGCGACAGCGTTTCAATGGAGGCGAGTTCTGTGAAGATGATGGAAGCCATGAAGGAGGTGTTCGAAGTTCCATTGGAGAGGAAGGTTCAGAATGTGTCTCAGAAGCCTTTCCATGGCTACTTCGGGCAGAACCCATTGATGCCTATCCATGAGAGCATGGGCATTGAAGACCCTCTTCTTCCCTCCAACATCCAATCTTTCTCCAACCTCATGTGGCCCTCCCGGCCAAACCCTCTGTTCTGGTAACTTTCTTCCCTTCATTCATTCTGCCTTTATTTCCCACTTTCAGAGCTAACCCTCATACTATTTTGCAGTGGAAACCTCAAATCCTACGCAGAATTGGTGTCCCAATTGGAGCAGACGGTGAAGAAGATGGTGTTCGAGAGCTACGGCCTCAAAAGCCTCTTCCTTTCTCACACCCAATCCACCAAATATCTTCTCCGGATGATCAAATACAGAGTCCCACATCGGAACGAGATGAACTTGGGAGCTTTCCCCCACACCGACAAGAGCTTCCTCACCATTCTTCATCAGAATTCAGTAAATGGGTTACACATTAAGACCAGAGACAACAGATGGGTTGAATATCAGCCTCCTTCTGCTCAATCTTCTGCCAGATCATTCATCGTCATGGCTGGAGATGCCTTCTTTGTAAGTTCCCAATAACTTCAAATTCCATTACAATTAGCAATAACCCATCAGAAACTTACAAAGAATTGTGGGGTTGTTGGGACAGGCGTGGAGCAATGGGAGGATATATTCGGCGCCGCACAGAGTGACGATGAGTGGAAACAGAGAGAGATACTCAGTGGGGCTGTTTTCGTTCAACGATGGGATTGTTCAGATTCCAAAAGAATTGGTGGACGACAAACATCCGCAGCAGTTTAAGGCGTTTGATCATCAAGATTACCTTCGTTTCTACAGCACTGAGAAAGGCCAGAGCTCCCCTTCTGCTATTAAAGCCTATTGTGGCGTTGCAAATGGCCTCCTTGATTCCCGAACTTGCATTCCCATGCATTAACTATTAACCCATCAAATTCCCATTATGCAAATGCTCTCTGTGTATAAAGTCATTACATTAGTATATGTTTGATAAGATCAAATTGTGTAGCTTGATCTTCTATTTGACAAGAAATTGGCTAACGTGTAGTTTTGATTGCTTTTAGCGCAAGCAACGTAAATGTGAGCTAAGAGAGATTGTTTCGAGTATATGAGTGAGATTGGGACATTGTGTGCTTGGGCGTTGACAAAGGGTTTCTCGACGAATCACCCCGACAAACCTCCACCAACAACTTTTTTCATCAAGAGAGACTTTGCTTACAATGTTCTTACTTTTCAATACTTTTCAGCATCAGAAAAAATTTATGTTCTTATAGTGTTTATGAACCGCTCAAGCCTACCGCTAGTAAATATTGTTATCTTTGGAGAGGTTTCTACACCTTTATAAAAGAATGTTTCGTTCCCC

mRNA sequence

AACAACAAAAACAGATGCCACTTTCAAAATTTGGGTTATAAAAAGGGTGTTTTAGTGCCACATTTTCCAAGCCAAAAACACAGACAACAAACACTTAACAGAGCTGAGTTTTAGTGTTCATCAATGGCGGGTGAGATTCCAGTTGTGAGATTTAGTTCACACAATGTGCAGAGCGGAGGGAGGAAGTGGGGTTCGACGAGGGAGAAGGTCCGGGAAGCACTTGAAGAATATGGGTGCTTTGTAGCGTTGTGCGACAGCGTTTCAATGGAGGCGAGTTCTGTGAAGATGATGGAAGCCATGAAGGAGGTGTTCGAAGTTCCATTGGAGAGGAAGGTTCAGAATGTGTCTCAGAAGCCTTTCCATGGCTACTTCGGGCAGAACCCATTGATGCCTATCCATGAGAGCATGGGCATTGAAGACCCTCTTCTTCCCTCCAACATCCAATCTTTCTCCAACCTCATGTGGCCCTCCCGGCCAAACCCTCTGTTCTGTGGAAACCTCAAATCCTACGCAGAATTGGTGTCCCAATTGGAGCAGACGGTGAAGAAGATGGTGTTCGAGAGCTACGGCCTCAAAAGCCTCTTCCTTTCTCACACCCAATCCACCAAATATCTTCTCCGGATGATCAAATACAGAGTCCCACATCGGAACGAGATGAACTTGGGAGCTTTCCCCCACACCGACAAGAGCTTCCTCACCATTCTTCATCAGAATTCAGTAAATGGGTTACACATTAAGACCAGAGACAACAGATGGGTTGAATATCAGCCTCCTTCTGCTCAATCTTCTGCCAGATCATTCATCGTCATGGCTGGAGATGCCTTCTTTGCGTGGAGCAATGGGAGGATATATTCGGCGCCGCACAGAGTGACGATGAGTGGAAACAGAGAGAGATACTCAGTGGGGCTGTTTTCGTTCAACGATGGGATTGTTCAGATTCCAAAAGAATTGGTGGACGACAAACATCCGCAGCAGTTTAAGGCGTTTGATCATCAAGATTACCTTCGTTTCTACAGCACTGAGAAAGGCCAGAGCTCCCCTTCTGCTATTAAAGCCTATTGTGGCGTTGCAAATGGCCTCCTTGATTCCCGAACTTGCATTCCCATGCATTAACTATTAACCCATCAAATTCCCATTATGCAAATGCTCTCTGTGTATAAAGTCATTACATTAGTATATGTTTGATAAGATCAAATTGTGTAGCTTGATCTTCTATTTGACAAGAAATTGGCTAACGTGTAGTTTTGATTGCTTTTAGCGCAAGCAACGTAAATGTGAGCTAAGAGAGATTGTTTCGAGTATATGAGTGAGATTGGGACATTGTGTGCTTGGGCGTTGACAAAGGGTTTCTCGACGAATCACCCCGACAAACCTCCACCAACAACTTTTTTCATCAAGAGAGACTTTGCTTACAATGTTCTTACTTTTCAATACTTTTCAGCATCAGAAAAAATTTATGTTCTTATAGTGTTTATGAACCGCTCAAGCCTACCGCTAGTAAATATTGTTATCTTTGGAGAGGTTTCTACACCTTTATAAAAGAATGTTTCGTTCCCC

Coding sequence (CDS)

ATGGCGGGTGAGATTCCAGTTGTGAGATTTAGTTCACACAATGTGCAGAGCGGAGGGAGGAAGTGGGGTTCGACGAGGGAGAAGGTCCGGGAAGCACTTGAAGAATATGGGTGCTTTGTAGCGTTGTGCGACAGCGTTTCAATGGAGGCGAGTTCTGTGAAGATGATGGAAGCCATGAAGGAGGTGTTCGAAGTTCCATTGGAGAGGAAGGTTCAGAATGTGTCTCAGAAGCCTTTCCATGGCTACTTCGGGCAGAACCCATTGATGCCTATCCATGAGAGCATGGGCATTGAAGACCCTCTTCTTCCCTCCAACATCCAATCTTTCTCCAACCTCATGTGGCCCTCCCGGCCAAACCCTCTGTTCTGTGGAAACCTCAAATCCTACGCAGAATTGGTGTCCCAATTGGAGCAGACGGTGAAGAAGATGGTGTTCGAGAGCTACGGCCTCAAAAGCCTCTTCCTTTCTCACACCCAATCCACCAAATATCTTCTCCGGATGATCAAATACAGAGTCCCACATCGGAACGAGATGAACTTGGGAGCTTTCCCCCACACCGACAAGAGCTTCCTCACCATTCTTCATCAGAATTCAGTAAATGGGTTACACATTAAGACCAGAGACAACAGATGGGTTGAATATCAGCCTCCTTCTGCTCAATCTTCTGCCAGATCATTCATCGTCATGGCTGGAGATGCCTTCTTTGCGTGGAGCAATGGGAGGATATATTCGGCGCCGCACAGAGTGACGATGAGTGGAAACAGAGAGAGATACTCAGTGGGGCTGTTTTCGTTCAACGATGGGATTGTTCAGATTCCAAAAGAATTGGTGGACGACAAACATCCGCAGCAGTTTAAGGCGTTTGATCATCAAGATTACCTTCGTTTCTACAGCACTGAGAAAGGCCAGAGCTCCCCTTCTGCTATTAAAGCCTATTGTGGCGTTGCAAATGGCCTCCTTGATTCCCGAACTTGCATTCCCATGCATTAA

Protein sequence

MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMKEVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNGRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKGQSSPSAIKAYCGVANGLLDSRTCIPMH

Homology

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BLAST of CmaCh13G009420 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.1e-64
Identity = 125/316 (39.56%), Postives = 194/316 (61.39%), Query Frame = 0


Query: 4   EIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMKEVF 63
           ++PV+ FS  N++ G  KW   +  V +ALE+YGCF A  D +S+E +   + EAM+++F
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNR-SVFEAMEDLF 72

Query: 64  EVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           E+P+  K +NVS KPFHGY   N    ++ES+GI+D  +   +  F+  +WP   N    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGIDDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 124 GNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVP-----HRNEM 183
             +  ++E + +L+  V++M+ ES+G+++    H  ST YL R++KY  P        E 
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEET 192

Query: 184 NLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWS 243
            LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S +VM GD+  A  
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCALL 252

Query: 244 NGRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFKAFDHQDYLRF 303
           NGR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ FK F++ D+L F
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 312

Query: 304 YSTEKGQSSPSAIKAY 313
           + TE G+ + SA+ A+
Sbjct: 313 FQTEAGRIAQSALHAF 318

[Close]

BLAST of CmaCh13G009420 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 9.3e-64
Identity = 125/317 (39.43%), Postives = 192/317 (60.57%), Query Frame = 0


Query: 4   EIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMKEVF 63
           ++PV+ FS  N++ G  KW      V +ALE+YGCF A  D +S+E +   + EAM+++F
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNR-SVFEAMEDLF 72

Query: 64  EVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           E+P+  K +NVS KPFHGY   N    ++ES+GI D  +   +  F+  +WP   N    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 124 GNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVP------HRNE 183
             +  ++E + +L+  V++M+ ES+G+++    H  ST YL R++KY  P         E
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 184 MNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAW 243
             LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S +VM GD+  A 
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCAL 252

Query: 244 SNGRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFKAFDHQDYLR 303
            NGR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ FK F++ D+L 
Sbjct: 253 LNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLH 312

Query: 304 FYSTEKGQSSPSAIKAY 313
           F+ TE G+ + SA+ A+
Sbjct: 313 FFQTEAGRIAQSALHAF 319

[Close]

BLAST of CmaCh13G009420 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 5.6e-61
Identity = 121/306 (39.54%), Postives = 184/306 (60.13%), Query Frame = 0


Query: 4   EIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMKEVF 63
           ++PV+ FS  N++ G  KW      V +ALE+YGCF A  D +S+E +   + EAM+++F
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNR-SVFEAMEDLF 65

Query: 64  EVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           E+P+  K +NVS K FHGY   N    ++ES+GI D  +   +  F+  +WP   N    
Sbjct: 66  ELPIPTKQRNVSSKLFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 125

Query: 124 GNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVP----HRNEMN 183
             +  ++E + +L+  V++M+ ES+G++     H  ST YL R++KY  P       E  
Sbjct: 126 ETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEETK 185

Query: 184 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSN 243
           LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S +VM GD+  A  N
Sbjct: 186 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCALLN 245

Query: 244 GRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFKAFDHQDYLRFY 303
           GR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ FK F++ D+L F+
Sbjct: 246 GRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFF 301

[Close]

BLAST of CmaCh13G009420 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 6.6e-54
Identity = 114/308 (37.01%), Postives = 177/308 (57.47%), Query Frame = 0


Query: 5   IPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMKEVFE 64
           +PV+  S  N++ G   W S R +VREALEEYGCF A+ D+VS E     +     E+  
Sbjct: 9   LPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKA-VCNKGHELLN 68

Query: 65  VPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFCG 124
           +PLE K+ N ++  + G+     +  ++E MG+        ++ F+NLMWP   N  FC 
Sbjct: 69  LPLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWP-EGNKDFCE 128

Query: 125 NLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFP 184
            + SY + +++++  +K MVFES+G++  F S  +ST YLL  ++Y+ P ++  +     
Sbjct: 129 TVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGKDGRSPALSL 188

Query: 185 HTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNGRIYS 244
           H DKS LTI++QN V GL  +T+D  W+         +A + IV+ GD F AWSNGR++S
Sbjct: 189 HKDKSILTIVNQNDVKGLEFETKDGEWI-------LPTADNHIVLLGDCFMAWSNGRLHS 248

Query: 245 APHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKGQS 304
             HRVT+  N+ R S   FSF   I++ P ELVD++HP  F  F+  + L +  T++G  
Sbjct: 249 PLHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKEGAK 304

Query: 305 SPSAIKAY 313
           +   +K Y
Sbjct: 309 AVCDLKQY 304

[Close]

BLAST of CmaCh13G009420 vs. ExPASy Swiss-Prot
Match: Q945B4 (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 2.9e-49
Identity = 126/414 (30.43%), Postives = 193/414 (46.62%), Query Frame = 0


Query: 4   EIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMKEVF 63
           ++P++  S   ++ G  KW   R  VR+ALE+YGCF A  D VSME     +++AM+E+F
Sbjct: 7   QLPLICLSDQTLKPGSSKWVKVRSDVRKALEDYGCFEAKIDQVSMELQG-SVLKAMQELF 66

Query: 64  EVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
            +P E K +NV  KPF GYF  N L    ES GI+D  +      F+  +WP     +  
Sbjct: 67  ALPTEAKQRNVCPKPFAGYFSHNGL---SESFGIKDANILEKAHEFTQQLWPEGNKSI-- 126

Query: 124 GNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEM----- 183
             ++ YAE +++L+  V++++ ESYG++     H  ST Y +R++KY     N++     
Sbjct: 127 KMIQLYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIARPDNDITAAVG 186

Query: 184 ------------------------------------------------------------ 243
                                                                       
Sbjct: 187 ANVDNGANDNADGDANVNDDGASIGVKVNVDVGDDVNDNDSVNIGVGVDINVETNVNGDL 246

Query: 244 -------------------------------NLGAFPHTDKSFLTILHQNSVNGLHIKTR 303
                                           LG   HTDKS   I++Q+ ++GL +KT+
Sbjct: 247 DAEANGDATAWVVGAVSGNASVGAKEANVDAELGLPSHTDKSLSGIIYQHQIDGLEVKTK 306

Query: 304 DNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNGRIYSAPHRVTMSGNRE-RYSVGLFSF- 319
           + +W+  +P     +  + IV+AGDA  A  NGRI S  HRV ++  ++ RY+  LFS+ 
Sbjct: 307 EGKWIRVKP-----APNTVIVIAGDALCALMNGRIPSPYHRVRVTERKKTRYAAALFSYP 366

[Close]

BLAST of CmaCh13G009420 vs. ExPASy TrEMBL
Match: A0A6J1KFF7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111495326 PE=3 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 3.5e-191
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0


Query: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK 60
           MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK
Sbjct: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK 60

Query: 61  EVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of CmaCh13G009420 vs. ExPASy TrEMBL
Match: A0A6J1E7C8 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431472 PE=3 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.1e-187
Identity = 320/329 (97.26%), Postives = 328/329 (99.70%), Query Frame = 0


Query: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK 60
           MAGE+PVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASS+KMMEAM+
Sbjct: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60

Query: 61  EVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVS+KPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFC NLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSA+SSA+SFI+MAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of CmaCh13G009420 vs. ExPASy TrEMBL
Match: A0A6J1EAT7 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111431473 PE=3 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 3.1e-187
Identity = 322/329 (97.87%), Postives = 327/329 (99.39%), Query Frame = 0


Query: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK 60
           MAGEIPVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVAL DSVSMEASS+KMMEAMK
Sbjct: 1   MAGEIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMK 60

Query: 61  EVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVS+KPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFC NLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSA+SSA+SFIVMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of CmaCh13G009420 vs. ExPASy TrEMBL
Match: A0A6J1C712 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008991 PE=3 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 5.3e-139
Identity = 239/326 (73.31%), Postives = 278/326 (85.28%), Query Frame = 0


Query: 4   EIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMKEVF 63
           ++P+V+FS  N+    R W STREKVREALEEYGCFVAL DSVSME SS  M +++KE+F
Sbjct: 5   QLPLVKFSEENL----RSWVSTREKVREALEEYGCFVALYDSVSMELSS-NMFDSLKELF 64

Query: 64  EVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           EVPLERK++NVS+KP+HGYFGQNPLMPIHESMGIEDP L S  QSF+NLMWPS  N  FC
Sbjct: 65  EVPLERKLKNVSEKPYHGYFGQNPLMPIHESMGIEDPNLLSKTQSFTNLMWPSNGNKSFC 124

Query: 124 GNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAF 183
            N+ SYA  +++L++TVKKMVFESYG++    SH +ST+YLLRMIKYRVP +NEMN+GAF
Sbjct: 125 ENVNSYARAIAELDETVKKMVFESYGVRKHLESHMKSTRYLLRMIKYRVPKQNEMNMGAF 184

Query: 184 PHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNGRIY 243
           PHTDKSFLTILHQN VNGL IKT+ N+W++Y P S+ SS  SFIVMAGDAFFAWSNGRIY
Sbjct: 185 PHTDKSFLTILHQNQVNGLQIKTKHNKWLQYHPSSSSSSTSSFIVMAGDAFFAWSNGRIY 244

Query: 244 SAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKGQ 303
           S PH+V MSG RERYSVGLFSFNDGIVQIPKELVD+ HPQQFK FDHQDYLRFYSTE+GQ
Sbjct: 245 SPPHKVIMSGKRERYSVGLFSFNDGIVQIPKELVDENHPQQFKPFDHQDYLRFYSTERGQ 304

Query: 304 SSPSAIKAYCGVANGLLDSRTCIPMH 330
           +SP AIKAYCG+ NGL+D+R CI +H
Sbjct: 305 NSPCAIKAYCGIGNGLVDTRGCILLH 325

[Close]

BLAST of CmaCh13G009420 vs. ExPASy TrEMBL
Match: A0A5D3BIZ7 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002910 PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 6.7e-134
Identity = 236/329 (71.73%), Postives = 279/329 (84.80%), Query Frame = 0


Query: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK 60
           M+ E+PVV+F S N++SG ++W  T +KVREALE+YGCFVAL DSVS E SS  M +++K
Sbjct: 5   MSDELPVVKFCSENLKSGTKEWVFTMKKVREALEDYGCFVALYDSVSAEVSS-NMFDSLK 64

Query: 61  EVF-EVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPN 120
           E+F +VPLERK+QNVS+KP+HGYFGQNPLMPIHESMGIE P+LP+NI SF+NLMWPSR N
Sbjct: 65  ELFDDVPLERKLQNVSEKPYHGYFGQNPLMPIHESMGIEHPILPTNINSFTNLMWPSRGN 124

Query: 121 PLFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMN 180
             F  ++ +YA+LVS+L++ VK+MVFESYG+ +   SH +STKYL+RMIKYRVP   EMN
Sbjct: 125 DSFRESVTTYAKLVSELDERVKRMVFESYGVGNALKSHMESTKYLMRMIKYRVPKEKEMN 184

Query: 181 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSN 240
           LGAFPHTDKSFLTILHQN VNGL IKTRDN+W++Y P S+ SS  SFIVMAGDAFFAWSN
Sbjct: 185 LGAFPHTDKSFLTILHQNEVNGLQIKTRDNKWIQYHPFSS-SSTSSFIVMAGDAFFAWSN 244

Query: 241 GRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYST 300
           GRIYS PHRV MSGNRERYSVGLFSFN+GIVQIP+ELVDDKHPQ F  FDHQD+LRFYST
Sbjct: 245 GRIYSPPHRVIMSGNRERYSVGLFSFNNGIVQIPEELVDDKHPQLFNPFDHQDFLRFYST 304

Query: 301 EKGQSSPSAIKAYCGVANGLLDSRTCIPM 329
           E+GQ+SP AIKAYCGV N L+D  T  P+
Sbjct: 305 ERGQNSPCAIKAYCGVGNSLIDMDTRSPL 331

[Close]

BLAST of CmaCh13G009420 vs. NCBI nr
Match: XP_023000968.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima] >XP_023001072.1 probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima])

HSP 1 Score: 677.2 bits (1746), Expect = 7.3e-191
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0


Query: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK 60
           MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK
Sbjct: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK 60

Query: 61  EVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of CmaCh13G009420 vs. NCBI nr
Match: XP_022923887.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 665.6 bits (1716), Expect = 2.2e-187
Identity = 320/329 (97.26%), Postives = 328/329 (99.70%), Query Frame = 0


Query: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK 60
           MAGE+PVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASS+KMMEAM+
Sbjct: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAME 60

Query: 61  EVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVS+KPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFC NLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSA+SSA+SFI+MAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIIMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of CmaCh13G009420 vs. NCBI nr
Match: KAG6584272.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 664.8 bits (1714), Expect = 3.7e-187
Identity = 320/329 (97.26%), Postives = 327/329 (99.39%), Query Frame = 0


Query: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK 60
           MAGE+PVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASS+KMMEAMK
Sbjct: 1   MAGELPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEAMK 60

Query: 61  EVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVS+KPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFC NLKSYA+LVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPS +SSA+SFIVMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of CmaCh13G009420 vs. NCBI nr
Match: XP_022923888.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata] >XP_023519885.1 probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita pepo subsp. pepo] >XP_023519886.1 probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 664.1 bits (1712), Expect = 6.4e-187
Identity = 322/329 (97.87%), Postives = 327/329 (99.39%), Query Frame = 0


Query: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK 60
           MAGEIPVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVAL DSVSMEASS+KMMEAMK
Sbjct: 1   MAGEIPVVRFSSHNLQSGGRKWGSTREKVREALEEYGCFVALYDSVSMEASSLKMMEAMK 60

Query: 61  EVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVS+KPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFC NLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSA+SSA+SFIVMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAESSAKSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of CmaCh13G009420 vs. NCBI nr
Match: KAG7019869.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 661.8 bits (1706), Expect = 3.2e-186
Identity = 318/329 (96.66%), Postives = 326/329 (99.09%), Query Frame = 0


Query: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMK 60
           MAGE+PVVRFSSHN+QSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASS+KMME MK
Sbjct: 1   MAGELPVVRFSSHNMQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSLKMMEGMK 60

Query: 61  EVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120
           EVFEVPLERKVQNVS+KPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP
Sbjct: 61  EVFEVPLERKVQNVSEKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNP 120

Query: 121 LFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180
           LFC NLKSYA+LVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL
Sbjct: 121 LFCENLKSYAQLVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNL 180

Query: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNG 240
           GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPS +SSA+SFIVMAGDAFFAWSNG
Sbjct: 181 GAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSTESSAKSFIVMAGDAFFAWSNG 240

Query: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTE 300
           RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQF+AFDHQDYLRFYSTE
Sbjct: 241 RIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFEAFDHQDYLRFYSTE 300

Query: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 330
           KGQSSPSAIKAYCGVANGLLDSRTCIPMH
Sbjct: 301 KGQSSPSAIKAYCGVANGLLDSRTCIPMH 329

[Close]

BLAST of CmaCh13G009420 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 282.0 bits (720), Expect = 6.3e-76
Identity = 136/313 (43.45%), Postives = 209/313 (66.77%), Query Frame = 0


Query: 5   IPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMKEVFE 64
           +PV+ FS+ N++ G  +W  TR  V++AL++YG F A  D +  E     +  A++E+F+
Sbjct: 12  LPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRK-SVFGALEELFD 71

Query: 65  VPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFCG 124
           +PL+ K++NVS+KPFHGY GQ P++P++ESMGI+D  +   + +F+  +WP + N  F  
Sbjct: 72  LPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWP-QGNISFST 131

Query: 125 NLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGAFP 184
            ++S+++ +S+L+ T+++M+ ES+GL      H  ST YLLR++KY+ P   E  +G   
Sbjct: 132 TIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTEETKVGLNA 191

Query: 185 HTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNGRIYS 244
           HTDK+ +TIL+QN V GL ++T+D  W++ +P     +  SF VM GD+ +A  NGR++S
Sbjct: 192 HTDKNIVTILYQNHVEGLEVQTKDKNWIKVKP-----TQDSFTVMIGDSLYALLNGRLHS 251

Query: 245 APHRVTMSGNRERYSVGLFSFNDG--IVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEKG 304
             HRV M+G   RYS+GLFS      IV  P ELVD++HP+ FK FDH ++L+FY TE G
Sbjct: 252 PYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAG 311

Query: 305 QSSPSAIKAYCGV 316
           Q S SA+K YCG+
Sbjct: 312 QRSQSALKTYCGI 317

[Close]

BLAST of CmaCh13G009420 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 280.4 bits (716), Expect = 1.8e-75
Identity = 134/314 (42.68%), Postives = 209/314 (66.56%), Query Frame = 0


Query: 4   EIPVVRFSS-HNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMKEV 63
           ++P++  +S   ++     W S   +  EALEEYGCF+A+ D V+ +     +  A +E+
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDD-SIFAAAEEL 68

Query: 64  FEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLF 123
           F++P E K +NV++KP+HGY GQ P++P+HE +G++        Q F++LMWP + N  F
Sbjct: 69  FDLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGNDRF 128

Query: 124 CGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMNLGA 183
           C  + +++  V++L++ V +M+FE+YG++  + SH  S  YLL+ +KY  P  + +++ A
Sbjct: 129 CNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPES-ISMPA 188

Query: 184 FP-HTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNGR 243
           FP HTDK+FL+ILHQN VNGL +K++D  W+  Q P      +S++VMAGD    WSN R
Sbjct: 189 FPQHTDKTFLSILHQNDVNGLEVKSKDGEWISLQLP-----PKSYVVMAGDISMGWSNDR 248

Query: 244 IYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYSTEK 303
           I S  HRVTM G++ RY++GLFSF   +V IP+ELVDDKHP  +K FD+   + FY+T++
Sbjct: 249 IRSCEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKE 308

Query: 304 GQSSPSAIKAYCGV 316
           G+ + S +KAYCG+
Sbjct: 309 GREANSTLKAYCGI 314

[Close]

BLAST of CmaCh13G009420 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 278.5 bits (711), Expect = 7.0e-75
Identity = 137/315 (43.49%), Postives = 205/315 (65.08%), Query Frame = 0


Query: 1   MAGEIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVA-LCDSVSMEASSVKMMEAM 60
           M+ +IP + FS  +++ G + W STRE +R+ALEEYGCF+  L D   ++    ++  ++
Sbjct: 1   MSPKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLD-RVFGSL 60

Query: 61  KEVFEVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPN 120
            ++F++P + K++N   KP +GY GQ P +P+HES+GI++       +SF+ LMWP + N
Sbjct: 61  VDLFDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP-QGN 120

Query: 121 PLFCGNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVPHRNEMN 180
             F   L  YAE  ++L+Q V +MVF+SY ++  +  + +ST YLLR++K R P+     
Sbjct: 121 EHFSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPT 180

Query: 181 LGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSN 240
           LG   HTDKSF TILHQ+ VNGL ++TR+   +        SS   F+V+AGDA  AWSN
Sbjct: 181 LGFVTHTDKSFTTILHQDQVNGLEMETREGERININ----LSSPSLFMVVAGDALMAWSN 240

Query: 241 GRIYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYST 300
            R++S  H+V +SG  +RYS+G+F+FN+G +Q+P+EL+D +HP  +K FDH   LRFY T
Sbjct: 241 DRVWSPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRT 300

Query: 301 EKGQSSPSAIKAYCG 315
           + G  S   IKAYCG
Sbjct: 301 DTGYKSECPIKAYCG 309

[Close]

BLAST of CmaCh13G009420 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 245.4 bits (625), Expect = 6.6e-65
Identity = 125/317 (39.43%), Postives = 192/317 (60.57%), Query Frame = 0


Query: 4   EIPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMKEVF 63
           ++PV+ FS  N++ G  KW      V +ALE+YGCF A  D +S+E +   + EAM+++F
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNR-SVFEAMEDLF 72

Query: 64  EVPLERKVQNVSQKPFHGYFGQNPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 123
           E+P+  K +NVS KPFHGY   N    ++ES+GI D  +   +  F+  +WP   N    
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCHN----LYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 124 GNLKSYAELVSQLEQTVKKMVFESYGLKSLFLSHTQSTKYLLRMIKYRVP------HRNE 183
             +  ++E + +L+  V++M+ ES+G+++    H  ST YL R++KY  P         E
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 184 MNLGAFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAW 243
             LG   HTDK+ +TILHQ  V+GL +KT+D++W++ +P     S  S +VM GD+  A 
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKP-----SQDSVLVMVGDSLCAL 252

Query: 244 SNGRIYSAPHRVTMSGNRERYSVGLFSF--NDGIVQIPKELVDDKHPQQFKAFDHQDYLR 303
            NGR++S  HRV M+G + RYS GLFS      I+  P+ELVD +HP+ FK F++ D+L 
Sbjct: 253 LNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLH 312

Query: 304 FYSTEKGQSSPSAIKAY 313
           F+ TE G+ + SA+ A+
Sbjct: 313 FFQTEAGRIAQSALHAF 319

[Close]

BLAST of CmaCh13G009420 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 238.8 bits (608), Expect = 6.2e-63
Identity = 129/317 (40.69%), Postives = 188/317 (59.31%), Query Frame = 0


Query: 5   IPVVRFSSHNVQSGGRKWGSTREKVREALEEYGCFVALCDSVSMEASSVKMMEAMKEVFE 64
           +P++ FSS  +  G   W +TR+ VR A+E  G FVA    VS +     ++  MKE++ 
Sbjct: 9   LPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRD-NLLAGMKEMYY 68

Query: 65  VPLERKVQNVSQKPFHGYFGQ-NPLMPIHESMGIEDPLLPSNIQSFSNLMWPSRPNPLFC 124
           +P + K++N + K  HGY         IHES+GI+        + FS L+WP   +P FC
Sbjct: 69  LPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWPQGNDP-FC 128

Query: 125 GNLKSYAELVSQLEQTVKKMVFESYGL--KSLFLSHTQSTKYLLRMIKYRVPHRNEMNLG 184
                YA  +++L+QTV +M++ESYG+  K   +SH++ST+YLLRM+ YR     E N G
Sbjct: 129 QTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEANTG 188

Query: 185 AFPHTDKSFLTILHQNSVNGLHIKTRDNRWVEYQPPSAQSSARSFIVMAGDAFFAWSNGR 244
              HTDKSF++ILHQN V GL +KT   +WV + P     S   F+V++G    AWSN R
Sbjct: 189 FVSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNP-----SPTRFVVLSGMGLTAWSNDR 248

Query: 245 IYSAPHRVTMSGNRERYSVGLFSFNDGIVQIPKELVDDKHPQQFKAFDHQDYLRFYST-- 304
           I +  H+V MS +  RYS+G FSF+ G ++ P+ELVDD+HP ++  F+H   LRFY +  
Sbjct: 249 IKACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYI 308

Query: 305 -EKGQSSPSAIKAYCGV 316
               +SS   ++ YCGV
Sbjct: 309 NSLKKSSEDLLQIYCGV 318

The following BLAST results are available for this feature:

BLAST of CmaCh13G009420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q945B6	1.1e-64	39.56	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q9ZTA3	9.3e-64	39.43	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
Q944Z5	5.6e-61	39.54	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
A0A0N9HQ36	6.6e-54	37.01	Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=...	[more]
Q945B4	2.9e-49	30.43	2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of CmaCh13G009420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KFF7	3.5e-191	100.00	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1E7C8	1.1e-187	97.26	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1EAT7	3.1e-187	97.87	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1C712	5.3e-139	73.31	probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Momordica chara...	[more]
A0A5D3BIZ7	6.7e-134	71.73	Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa O...	[more]

BLAST of CmaCh13G009420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023000968.1	7.3e-191	100.00	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima] >XP_023001...	[more]
XP_022923887.1	2.2e-187	97.26	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata]	[more]
KAG6584272.1	3.7e-187	97.26	putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyros...	[more]
XP_022923888.1	6.4e-187	97.87	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata] >XP_0235...	[more]
KAG7019869.1	3.2e-186	96.66	putative 2-oxoglutarate-dependent dioxygenase AOP1.2, partial [Cucurbita argyros...	[more]

BLAST of CmaCh13G009420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52820.1	6.3e-76	43.45	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52800.1	1.8e-75	42.68	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52790.1	7.0e-75	43.49	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G03070.1	6.6e-65	39.43	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G80320.1	6.2e-63	40.69	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 3..317 e-value: 5.5E-52 score: 179.0
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 5..84 e-value: 3.4E-7 score: 31.0
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 170..265 e-value: 6.5E-18 score: 65.1
None	No IPR available	PANTHER	PTHR47990:SF17	2-OXOGLUTARATE-DEPENDENT DIOXYGENASE AOP3-LIKE	coord: 4..314
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 4..314
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 4..302
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 161..268 score: 10.739458

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh13G009420.1	CmaCh13G009420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding