CmaCh13G008710 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh13G008710
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	short-chain dehydrogenase TIC 32, chloroplastic-like
Location	Cma_Chr13: 7111999 .. 7114986 (+)
RNA-Seq Expression	CmaCh13G008710
Synteny	CmaCh13G008710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGAAGAGAGAGAGCGAGAGGTTGAATGGTAGGGTGATGAAGGAGACTCTAAGGTATTTGGTTGGAATTGCAGGCCCAAGTGGGTATGGCTCTAAGTCAAGTGCAGAGCAAGTTACTGAACATTTGCAGTGTTCTTCTCCTTTGACTGCCATTATCACTGGTAAGCTTTTTTTTTTAACGATAGTAACCTAATTTTGGTCTTTTCTTACTGTTTTGTTCTAAATTCACTTTGTTCTGCAAGTTGTATCCTAACTAATATATGGTTCTTTTGAATGGTACCAAGGGTTGATTTTTTTTTTTTTTTTAAATGAATTACTGTTAAGCTAGGCTCGAATTATCTTTAAAGTTGTGGCCATTCAATTTAAGGTAGAATAAAGAAAAAAACCACAAAAAGGGTTCGAACCAAAGTTGCACATGTATATATGAGAGAATTTAGGTTTTTCTTTCATTTTCCTAAATTTTATTAGGAAGAAACTAAATCTATAAAATCTTGTTTGCTAATCATTTAGTTTTCTTTTTTTTTTTTCTTTTTTTTTTTAATTTTTAAATCAAGCTTATAAATATTACTCAACCCCTAATTTCTTTGTTTTGTTATATATATATATATATATATTTAAAAAAAAAATTAAAAAGAGCTAAAATTTAATTTTTTAAAGAGAGACGAGAATAAAAAAGAAATCGTGAGAAAACAAACCCAATTTTCAAAAATAAAAATAAAAATCATTATAAAATGGAGTTTTACCTTTTGATCAATTGAATTATGGTCGGCTTGGTTGTCTTCAGCTCGAGGCTTCATTTCCCTAAATTTGTCAGGAGGGCGTTCGAAACTAAATGGATTTAACCAAATCGCTTCTTGTATCCACCTCCAAACCTTAATCCGTACATCCTATACATATATACACATACTTATATGAGAAGCTTAATTCTATAAATATAAGGAAGGAAATAAGGAGAAAAACCACTCAGCATGATCAATTAAACCATATAATGTTACCAAAGAATAATAGATGGGTGTTAAAACAAGACAAAACAATCCAGAAGAATGATGAGAATTTGATTTTATTTGTGGGTGCAAAAAGGGGCAACATCAGGAATTGGAGAAGAAACAGCAAGAGTTTTAGCAAAAAGAGGAGTGAGAATAGTAATGGCAGCAAGAGACATGAAGAAGGCAGCAAAAGTGAGAGAGAGGATCAAAAGAGAAGGTCCAGAAGCAGAGATTTTAGTGTCTGAGATAGACCTCAGCTCTTTGGCTTCTGTTAGGAGGTTCTGTATGGAGTTTGCAGCTTTAGGGCTACCACTCAACATTCTCATGTGAGTTTGCCCCCACTTTCCCCAACCAATATCCCATTTTTCCCTCTCAAATTTCACCCTTTTTGGACACACAAACTGTTTTTGCAGAAACAATGGTGGGGTTTTCTCTCCAAATTTGGAGTATTCTGATGACAAGCTTGAGTTGACATTTGCTACTAATTATTTGGGTAATAGTTTTTCCTTCTCTCTTTTTTCTTCTCATTCTGAAACTCAAACACAGAACAACAGTTTGATTGCAGAAGAATAGATGATTCTTAGCTCTACAAACTCCTTAATTACTTTTCCAAAGTCAGAGAAAGTTGAATCTACTCAATAATGGCTAAAGAAGAATGAAGTTACGTCAAAAAAAAGGATACAAAATGAATTTCAAAATTCTTTTTCCTGCAGGACATTACCTTCTGACAGAACTGCTTTTAGACAAATTGATAGAAACATCAAGGAGAACAGGCATCCAAGGAAGAATTATAAACGTCTCTTCTGTTGTCCACTGCTGGGTGAAGAAGGACGATCTCTGCTTCACTCACCTGCTCAACCCAAACAAGTTAGAAAAACTCGAACCCATTACAGATCATTTTCGTTTAGGTTTCGAATTTTCAAGTTTGTGTTTATATGATTCAAAAGGATCAGATAGAAACGCAAACTTTTAATTTTGTGTCCAATAAGTCAAAAAGAACTTTTAAAAGTGCGCACAGAAAGTTTAAGAAATCCATTTTGAGTAACTAAAACTTTAATTTCTTTCTCCCTTTTCTCAAAACACAAAAAATTACCAAGGTGTTCACTAGCTGGCTACAGGTCAATAGCAATCCATGGTCAGAGAATCGAACTAAACCCTGTTTGGGGTTTTGGATTTGGAATGTCTGCAGGTACAATGGCACGCGTGCTTATGCTCAATCAAAACTGGCTTGCATTTTACATGCCAAGGAGGTGGCTAGACAGTTGAAGGTAATCTTGATTCTTCACCTTCTACGTTTCCTGCAAACAGTTAACAAAACAGTCCATATTCTGAAATTCCAGCCATTTTTACAGGAAAGAGATGCCAATGTGACAATTAATGCAGTGCATCCAGGCATTGTAAAGACTGCAATTATCAAGGCACATCGAGGATTTATCACAGGCATGAACTGTTGACTCTTTCTAATTCCTGATGTACAGCCATCTTGAGCCAGTTCTACTTCTACCAAAAGGAAAATGTGCAAGAGAGTACTGATTTATCATAACTATATTCTAACATCTCTTGCAGATTCTCTCTTTTTCATGGCATCCAAGTTACTAAAATCCATTCCTCAGGTATTTTCATTTGTAAAATCGATTCAGAGCGTTTGGATTTGCCTAGTGTTACTCATACAAAACAGATAGTAACAATTATTGTGCCTACAGGGTGCTGCTACGACATGCTATGTGGCTCTAAGCCCACAAACACAGAGAAAAACAGGAAGATATTTTGCAGATTGTAATGAGACTAAGTGTTCATCTCTAGCCAACGATGAGTTAGCAGCACGAACGCTATGGATGCAAACTCGTGCCCTAATTCTCAGAGAACTTGGTGAACCAGTTTGAGCTACATCATCAATAAATTTTCAACCAAGAGAGAGAGAGAACAGTATATATAATAAATCTATGCATTTGTAAATGAACAGAGATCCTGGAGAAGTTCATCGAATAAGATCTCGTAAT

mRNA sequence

ATGTGGAAGAGAGAGAGCGAGAGGTTGAATGGTAGGGTGATGAAGGAGACTCTAAGGTATTTGGTTGGAATTGCAGGCCCAAGTGGGTATGGCTCTAAGTCAAGTGCAGAGCAAGTTACTGAACATTTGCAGTGTTCTTCTCCTTTGACTGCCATTATCACTGGGGCAACATCAGGAATTGGAGAAGAAACAGCAAGAGTTTTAGCAAAAAGAGGAGTGAGAATAGTAATGGCAGCAAGAGACATGAAGAAGGCAGCAAAAGTGAGAGAGAGGATCAAAAGAGAAGGTCCAGAAGCAGAGATTTTAGTGTCTGAGATAGACCTCAGCTCTTTGGCTTCTGTTAGGAGGTTCTGTATGGAGTTTGCAGCTTTAGGGCTACCACTCAACATTCTCATAAACAATGGTGGGGTTTTCTCTCCAAATTTGGAGTATTCTGATGACAAGCTTGAGTTGACATTTGCTACTAATTATTTGGGACATTACCTTCTGACAGAACTGCTTTTAGACAAATTGATAGAAACATCAAGGAGAACAGGCATCCAAGGAAGAATTATAAACGTCTCTTCTGTTGTCCACTGCTGGGTCAATAGCAATCCATGGTCAGAGAATCGAACTAAACCCTGTTTGGGGTACAATGGCACGCGTGCTTATGCTCAATCAAAACTGGCTTGCATTTTACATGCCAAGGAGGTGGCTAGACAGTTGAAGGAAAGAGATGCCAATGTGACAATTAATGCAGTGCATCCAGGCATTGTAAAGACTGCAATTATCAAGGCACATCGAGGATTTATCACAGGCATGAACTATTCTCTCTTTTTCATGGCATCCAAGTTACTAAAATCCATTCCTCAGGGTGCTGCTACGACATGCTATGTGGCTCTAAGCCCACAAACACAGAGAAAAACAGGAAGATATTTTGCAGATTGTAATGAGACTAAGTGTTCATCTCTAGCCAACGATGAGTTAGCAGCACGAACGCTATGGATGCAAACTCGTGCCCTAATTCTCAGAGAACTTGGTGAACCAGTTTGAGCTACATCATCAATAAATTTTCAACCAAGAGAGAGAGAGAACAGTATATATAATAAATCTATGCATTTGTAAATGAACAGAGATCCTGGAGAAGTTCATCGAATAAGATCTCGTAAT

Coding sequence (CDS)

ATGTGGAAGAGAGAGAGCGAGAGGTTGAATGGTAGGGTGATGAAGGAGACTCTAAGGTATTTGGTTGGAATTGCAGGCCCAAGTGGGTATGGCTCTAAGTCAAGTGCAGAGCAAGTTACTGAACATTTGCAGTGTTCTTCTCCTTTGACTGCCATTATCACTGGGGCAACATCAGGAATTGGAGAAGAAACAGCAAGAGTTTTAGCAAAAAGAGGAGTGAGAATAGTAATGGCAGCAAGAGACATGAAGAAGGCAGCAAAAGTGAGAGAGAGGATCAAAAGAGAAGGTCCAGAAGCAGAGATTTTAGTGTCTGAGATAGACCTCAGCTCTTTGGCTTCTGTTAGGAGGTTCTGTATGGAGTTTGCAGCTTTAGGGCTACCACTCAACATTCTCATAAACAATGGTGGGGTTTTCTCTCCAAATTTGGAGTATTCTGATGACAAGCTTGAGTTGACATTTGCTACTAATTATTTGGGACATTACCTTCTGACAGAACTGCTTTTAGACAAATTGATAGAAACATCAAGGAGAACAGGCATCCAAGGAAGAATTATAAACGTCTCTTCTGTTGTCCACTGCTGGGTCAATAGCAATCCATGGTCAGAGAATCGAACTAAACCCTGTTTGGGGTACAATGGCACGCGTGCTTATGCTCAATCAAAACTGGCTTGCATTTTACATGCCAAGGAGGTGGCTAGACAGTTGAAGGAAAGAGATGCCAATGTGACAATTAATGCAGTGCATCCAGGCATTGTAAAGACTGCAATTATCAAGGCACATCGAGGATTTATCACAGGCATGAACTATTCTCTCTTTTTCATGGCATCCAAGTTACTAAAATCCATTCCTCAGGGTGCTGCTACGACATGCTATGTGGCTCTAAGCCCACAAACACAGAGAAAAACAGGAAGATATTTTGCAGATTGTAATGAGACTAAGTGTTCATCTCTAGCCAACGATGAGTTAGCAGCACGAACGCTATGGATGCAAACTCGTGCCCTAATTCTCAGAGAACTTGGTGAACCAGTTTGA

Protein sequence

MWKRESERLNGRVMKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGVRIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHCWVNSNPWSENRTKPCLGYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIKAHRGFITGMNYSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETKCSSLANDELAARTLWMQTRALILRELGEPV

Homology

[Close]

BLAST of CmaCh13G008710 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 8.4e-68
Identity = 145/308 (47.08%), Postives = 199/308 (64.61%), Query Frame = 0


Query: 21  LVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGVRIVMAAR 80
           + G AG SGYGS S+AE VT  +  +  LTAIITG TSGIG E ARVL  RG  +++A+R
Sbjct: 7   VTGKAGKSGYGSASTAEDVTHSID-AKHLTAIITGGTSGIGLEAARVLGMRGAHVIIASR 66

Query: 81  DMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFSP 140
           + K A   +E I +  P A I   ++DLSS+ SVR F  +F AL +PLNILINN GV   
Sbjct: 67  NTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFC 126

Query: 141 NLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHCWVNSNPW 200
             + S+D +E  FATN++GH+LLT LLLDK+  ++R +GI+GRI+N+SS+ H +  +   
Sbjct: 127 PFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGI 186

Query: 201 SENRTKPCLGYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIKAH 260
             +       Y+  +AY QSKLA +LH+  ++R+L+E   N+TIN+VHPG++ T + + H
Sbjct: 187 MFDYINDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFR-H 246

Query: 261 RGFITGMNYSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETKCSSLAND 320
            G    +  ++ F    L K+IPQGAATTCYVAL P  +  TG+YFADCN T  S+ A D
Sbjct: 247 SGLGMAVLKAMSFF---LWKNIPQGAATTCYVALHPDLKDVTGKYFADCNVTTPSNFAID 306

Query: 321 ELAARTLW 329
              A  LW
Sbjct: 307 TTLADKLW 309

[Close]

BLAST of CmaCh13G008710 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.1e-67
Identity = 145/308 (47.08%), Postives = 198/308 (64.29%), Query Frame = 0


Query: 21  LVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGVRIVMAAR 80
           + G AG SGYGS S+AE VT  +  +  LTAIITG TSGIG E ARVL  RG  +++AAR
Sbjct: 7   VTGKAGKSGYGSASTAEDVTHSID-AKHLTAIITGGTSGIGLEAARVLGMRGAHVIIAAR 66

Query: 81  DMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFSP 140
           + K A   +E I +  P A I   ++DLSS+ SVR F  +F AL +PLNILINN GV   
Sbjct: 67  NTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFC 126

Query: 141 NLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHCWVNSNPW 200
             + S+D +E  FATN++GH+LLT LLLDK+  ++R +GI+GRI+N+SS+ H +  +   
Sbjct: 127 PFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGI 186

Query: 201 SENRTKPCLGYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIKAH 260
             +       Y+  +AY QSKLA +LH+  ++R+L+E   N+TIN+VHPG++ T + + H
Sbjct: 187 MFDYINDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFR-H 246

Query: 261 RGFITGMNYSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETKCSSLAND 320
            G    +  ++ F    L K+IPQGAATTCYVAL P  +  TG+YF DCN T  S+ A D
Sbjct: 247 SGLGMAVLKAMSFF---LWKNIPQGAATTCYVALHPDLKGVTGKYFTDCNVTTPSNFAID 306

Query: 321 ELAARTLW 329
              A  LW
Sbjct: 307 TTLADKLW 309

[Close]

BLAST of CmaCh13G008710 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 1.4e-67
Identity = 147/322 (45.65%), Postives = 204/322 (63.35%), Query Frame = 0


Query: 20  YLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGVRIVMAA 79
           +  G  G SG+ S+S+AE+VT H    + LTAI+TGA+SGIG ETARVL+ RGV +VMA 
Sbjct: 2   WFFGSKGASGFSSRSTAEEVT-HGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAV 61

Query: 80  RDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFS 139
           R+    AKV+E I ++ P A++ V E+DLSS+ SVR+F  E+ + GLPLN+LINN G+ +
Sbjct: 62  RNTDSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMA 121

Query: 140 PNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHCWVNSNP 199
                S D +EL FATN+LGH+LLT+LLLD +  TSR +  +GRI+N+SS  H +     
Sbjct: 122 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEG 181

Query: 200 WSENRTKPCLGYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIKA 259
              ++      Y+  RAY QSKL  +LHA E+ +QLKE   N+T N++HPG + T +   
Sbjct: 182 VRFDKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNL--- 241

Query: 260 HRGFITGMNYSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETKCSSLAN 319
            R F   +  ++  +A  +LKS+PQGAATTCYVAL+PQ    +G YF D N  K   L  
Sbjct: 242 GRYFNPYLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVK 301

Query: 320 DELAARTLWMQTRALILRELGE 342
           D   A+ +W  +  L   + GE
Sbjct: 302 DTELAKKVWDFSTKLTDSQSGE 319

[Close]

BLAST of CmaCh13G008710 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 2.8e-63
Identity = 133/303 (43.89%), Postives = 191/303 (63.04%), Query Frame = 0


Query: 26  GPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGVRIVMAARDMKKA 85
           G SG+   S+AEQVT  +  +  LTAI+TGA+SGIG ET RVLA RG  ++M  R+M  A
Sbjct: 9   GVSGFSGSSTAEQVTHGIDATG-LTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMVAA 68

Query: 86  AKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFSPNLEYS 145
             V++ I ++ P A++   E+DLSSL SV++F  EF + G PLNILINN G+ +   + S
Sbjct: 69  KDVKDTILKDIPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPFKLS 128

Query: 146 DDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHCWVNSNPWSENRT 205
            D +EL FATN++GH+LLT LLLD + +T+R +  +GRI+NV+S  H +        ++ 
Sbjct: 129 KDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRFDKI 188

Query: 206 KPCLGYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIKAHRGFIT 265
                YN  RAY QSKLA +LHA ++ + LKE   N+T N++HPG + T + + H   + 
Sbjct: 189 NDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFR-HNSAVN 248

Query: 266 GMNYSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETKCSSLANDELAAR 325
           G+   +  +   +LK++ QGAATTCYVAL PQ +  +G YF+D N  K +    D   A+
Sbjct: 249 GL---INVIGKLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTPHGKDVDLAK 306

Query: 326 TLW 329
            LW
Sbjct: 309 KLW 306

[Close]

BLAST of CmaCh13G008710 vs. ExPASy Swiss-Prot
Match: Q9HBH5 (Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 2.6e-40
Identity = 111/293 (37.88%), Postives = 164/293 (55.97%), Query Frame = 0


Query: 50  TAIITGATSGIGEETARVLAKRGVRIVMAARDMKKAAKVRERIKRE-------GPE---- 109
           T +ITGA SG+G  TA  L + G R++M  RD  +A +   +++RE       GPE    
Sbjct: 45  TVLITGANSGLGRATAAELLRLGARVIMGCRDRARAEEAAGQLRRELRQAAECGPEPGVS 104

Query: 110 --AEILVSEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFSPNLEYSDDKLELTFATN 169
              E++V E+DL+SL SVR FC E       L++LINN G+F      ++D  E+ F  N
Sbjct: 105 GVGELIVRELDLASLRSVRAFCQEMLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQFGVN 164

Query: 170 YLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHCWVNSNPWSENRTKPCLGYNGTRA 229
           +LGH+LLT LLL  L     ++    RI+ VSS ++ + + N    N  +    YN +  
Sbjct: 165 HLGHFLLTNLLLGLL-----KSSAPSRIVVVSSKLYKYGDINFDDLNSEQ---SYNKSFC 224

Query: 230 YAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIK-AHRGFITGMNYSLFFMA 289
           Y++SKLA IL  +E+AR+L+    NVT+N +HPGIV+T + +  H   +    ++L   A
Sbjct: 225 YSRSKLANILFTRELARRLE--GTNVTVNVLHPGIVRTNLGRHIHIPLLVKPLFNLVSWA 284

Query: 290 SKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETKCSSLANDELAARTLW 329
               K+  +GA T+ Y+A SP+ +  +GRYF DC E +    A DE  AR LW
Sbjct: 285 --FFKTPVEGAQTSIYLASSPEVEGVSGRYFGDCKEEELLPKAMDESVARKLW 325

[Close]

BLAST of CmaCh13G008710 vs. ExPASy TrEMBL
Match: A0A6J1KGJ5 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495058 PE=3 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 2.0e-173
Identity = 324/343 (94.46%), Postives = 325/343 (94.75%), Query Frame = 0


Query: 1   MWKRESERLNGRVMKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGI 60
           MWKRESERLNGRVMKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGI
Sbjct: 1   MWKRESERLNGRVMKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGI 60

Query: 61  GEETARVLAKRGVRIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCME 120
           GEETARVLAKRGVRIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCME
Sbjct: 61  GEETARVLAKRGVRIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCME 120

Query: 121 FAALGLPLNILINNGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGI 180
           FAALGLPLNILINNGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGI
Sbjct: 121 FAALGLPLNILINNGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGI 180

Query: 181 QGRIINVSSVVHCWVNSNPWSENRTKPCLGYNGTRAYAQSKLACILHAKEVARQLKERDA 240
           QGRIINVSSVVHCWV  +            YNGTRAYAQSKLACILHAKEVARQLKERDA
Sbjct: 181 QGRIINVSSVVHCWVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDA 240

Query: 241 NVTINAVHPGIVKTAIIKAHRGFITGMNYSLFFMASKLLKSIPQGAATTCYVALSPQTQR 300
           NVTINAVHPGIVKTAIIKAHRGFIT    SLFFMASKLLKSIPQGAATTCYVALSPQTQR
Sbjct: 241 NVTINAVHPGIVKTAIIKAHRGFITD---SLFFMASKLLKSIPQGAATTCYVALSPQTQR 300

Query: 301 KTGRYFADCNETKCSSLANDELAARTLWMQTRALILRELGEPV 344
           KTGRYFADCNETKCSSLANDELAARTLWMQTRALILRELGEPV
Sbjct: 301 KTGRYFADCNETKCSSLANDELAARTLWMQTRALILRELGEPV 340

[Close]

BLAST of CmaCh13G008710 vs. ExPASy TrEMBL
Match: A0A6J1EBB9 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111431615 PE=3 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.6e-170
Identity = 318/343 (92.71%), Postives = 322/343 (93.88%), Query Frame = 0


Query: 1   MWKRESERLNGRVMKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGI 60
           MWKRESERL GRVMKETLRYLVG+AGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGI
Sbjct: 1   MWKRESERLKGRVMKETLRYLVGMAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGI 60

Query: 61  GEETARVLAKRGVRIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCME 120
           GEETARVLAKRGVRIVMAARDMKKAAKVRERIKR GPEAEIL+SEIDLSSLASVRRFCME
Sbjct: 61  GEETARVLAKRGVRIVMAARDMKKAAKVRERIKRGGPEAEILMSEIDLSSLASVRRFCME 120

Query: 121 FAALGLPLNILINNGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGI 180
           FAALGLPLNILINNGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGI
Sbjct: 121 FAALGLPLNILINNGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGI 180

Query: 181 QGRIINVSSVVHCWVNSNPWSENRTKPCLGYNGTRAYAQSKLACILHAKEVARQLKERDA 240
           QGRIINVSSVVHCWV  +            YNGTRAYAQSKLACILHAKEVARQLKERDA
Sbjct: 181 QGRIINVSSVVHCWVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDA 240

Query: 241 NVTINAVHPGIVKTAIIKAHRGFITGMNYSLFFMASKLLKSIPQGAATTCYVALSPQTQR 300
           NVTINAVHPGIVKTAIIKAHRGFIT    SLFFMASKLLKSIPQGAATTCYVALSPQT R
Sbjct: 241 NVTINAVHPGIVKTAIIKAHRGFITD---SLFFMASKLLKSIPQGAATTCYVALSPQTHR 300

Query: 301 KTGRYFADCNETKCSSLANDELAARTLWMQTRALILRELGEPV 344
           KTGRYFADCNETKCSSLANDELAARTLW+QTRALILRELGEPV
Sbjct: 301 KTGRYFADCNETKCSSLANDELAARTLWIQTRALILRELGEPV 340

[Close]

BLAST of CmaCh13G008710 vs. ExPASy TrEMBL
Match: A0A6J1C5L3 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111008680 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 3.4e-144
Identity = 272/328 (82.93%), Postives = 293/328 (89.33%), Query Frame = 0


Query: 14  MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73
           MKETLRYL GIAG SGYGSKS+AEQVT+H   SSPLTAIITGATSGIGEETARV+AKRGV
Sbjct: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV 60

Query: 74  RIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 133
           RIV+AARD+KKAAKV+ERIKRE  EAEILVSEIDLSSLASVRRFC++FAA GLPLNILIN
Sbjct: 61  RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN 120

Query: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193
           N GVFSPNLEYSDDK ELTFATNYLGHYLLTE+LLD LIETS+RTGIQGRIINVSSVVHC
Sbjct: 121 NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC 180

Query: 194 WVNSNPWSENRTKPCLGYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253
           WV  +            YN T AYAQSKLACILHAKEVARQLKER ANVT+NA+HPGIVK
Sbjct: 181 WVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVK 240

Query: 254 TAIIKAHRGFITGMNYSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETK 313
           TAII+AH GFIT    SLFFMASKLLKSIPQGAATTCYVAL+PQT+RKTG+YF+DCNETK
Sbjct: 241 TAIIRAHGGFITD---SLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETK 300

Query: 314 CSSLANDELAARTLWMQTRALILRELGE 342
           CS+LAN+ELAAR LWMQTRALI R+LGE
Sbjct: 301 CSALANNELAARKLWMQTRALIHRQLGE 325

[Close]

BLAST of CmaCh13G008710 vs. ExPASy TrEMBL
Match: A0A6J1BYZ0 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111006882 PE=3 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 4.1e-126
Identity = 241/337 (71.51%), Postives = 283/337 (83.98%), Query Frame = 0


Query: 11  GRVMKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSS-----PLTAIITGATSGIGEETA 70
           G++MKETLRYL GIAGPSGYGS S+AEQVT++   SS     PLTAIITGATSGIG ETA
Sbjct: 12  GKLMKETLRYLAGIAGPSGYGSNSTAEQVTQNYSISSSSTSHPLTAIITGATSGIGAETA 71

Query: 71  RVLAKRGVRIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALG 130
           RVLAKRGVRIVMAARD+KKAA+V+E I++E PEAEI+V EIDLSSLASV+RFC +F ALG
Sbjct: 72  RVLAKRGVRIVMAARDLKKAAQVKEGIQKETPEAEIIVCEIDLSSLASVQRFCNQFLALG 131

Query: 131 LPLNILINNGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRII 190
           LPLNILINN GVFS NLE+S+DK+ELTFATNYLGHY LTE LL+K+IET+ +TGI+GRII
Sbjct: 132 LPLNILINNAGVFSKNLEFSEDKVELTFATNYLGHYFLTETLLEKMIETAAKTGIEGRII 191

Query: 191 NVSSVVHCWVNSNPWSENRTKPCLGYNGTRAYAQSKLACILHAKEVARQLKERDANVTIN 250
           NVSSV+HCWV  +     +      YNGTRAYAQSKLA ILHAKE++RQL+ R+A VTIN
Sbjct: 192 NVSSVIHCWVKKDGLRFGQMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQARNARVTIN 251

Query: 251 AVHPGIVKTAIIKAHRGFITGMNYSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRY 310
           AVHPGIVKTAII+AH+GFIT    SLFFMASKLLK+  QGA+TTCYVALS QT+ K+G++
Sbjct: 252 AVHPGIVKTAIIRAHKGFITD---SLFFMASKLLKTTSQGASTTCYVALSSQTEGKSGKF 311

Query: 311 FADCNETKCSSLANDELAARTLWMQTRALILRELGEP 343
           +ADCNET CS+LA+DEL A+ LW +T  LI R L +P
Sbjct: 312 YADCNETNCSALASDELEAQKLWTKTHNLIHRRLSKP 345

[Close]

BLAST of CmaCh13G008710 vs. ExPASy TrEMBL
Match: A0A2P5FFY3 (Short-chain dehydrogenase/reductase OS=Trema orientale OX=63057 GN=TorRG33x02_073970 PE=3 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 1.0e-124
Identity = 234/331 (70.69%), Postives = 272/331 (82.18%), Query Frame = 0


Query: 14  MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSP--LTAIITGATSGIGEETARVLAKR 73
           MKETLRYL GIAGPSGYGSKS+AEQVT +   S P  LTAIITGATSGIG ETARVLAKR
Sbjct: 1   MKETLRYLTGIAGPSGYGSKSTAEQVTANCSSSLPSHLTAIITGATSGIGAETARVLAKR 60

Query: 74  GVRIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNIL 133
           GVR+V+ ARD+ +AA+V+E I+RE P+AE++V EIDLSS ASV+RFC EF ALGLPLNIL
Sbjct: 61  GVRVVIPARDLIRAAEVKEGIQRETPKAEVIVLEIDLSSFASVKRFCSEFLALGLPLNIL 120

Query: 134 INNGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVV 193
           INN G+FS  LEYSDDK+E+TFATNYLGHYLLT+LLL+K++ET+ +TG+QGRIINVSS++
Sbjct: 121 INNAGIFSQTLEYSDDKIEMTFATNYLGHYLLTDLLLEKMVETAAQTGVQGRIINVSSMI 180

Query: 194 HCWVNSNPWSENRTKPCLGYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGI 253
           H WV  + +          YNGTRAYAQSKLA +LHAKE+ARQLK R+A VTINAVHPGI
Sbjct: 181 HSWVKKDTFRFTEMLIPKSYNGTRAYAQSKLASVLHAKEIARQLKARNARVTINAVHPGI 240

Query: 254 VKTAIIKAHRGFITGMNYSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNE 313
           VKT II+AH+  ITG  YS+FFMASKLLKS+ QGAATTCYVALSPQT+   G+YF DCNE
Sbjct: 241 VKTGIIRAHKSIITG-KYSVFFMASKLLKSVSQGAATTCYVALSPQTEEVNGKYFVDCNE 300

Query: 314 TKCSSLANDELAARTLWMQTRALILRELGEP 343
           + CS LANDE  A  LW QTRAL+ R L  P
Sbjct: 301 SSCSPLANDESEAHNLWKQTRALLHRRLCHP 330

[Close]

BLAST of CmaCh13G008710 vs. NCBI nr
Match: XP_023000696.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 618.2 bits (1593), Expect = 4.2e-173
Identity = 324/343 (94.46%), Postives = 325/343 (94.75%), Query Frame = 0


Query: 1   MWKRESERLNGRVMKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGI 60
           MWKRESERLNGRVMKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGI
Sbjct: 1   MWKRESERLNGRVMKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGI 60

Query: 61  GEETARVLAKRGVRIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCME 120
           GEETARVLAKRGVRIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCME
Sbjct: 61  GEETARVLAKRGVRIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCME 120

Query: 121 FAALGLPLNILINNGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGI 180
           FAALGLPLNILINNGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGI
Sbjct: 121 FAALGLPLNILINNGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGI 180

Query: 181 QGRIINVSSVVHCWVNSNPWSENRTKPCLGYNGTRAYAQSKLACILHAKEVARQLKERDA 240
           QGRIINVSSVVHCWV  +            YNGTRAYAQSKLACILHAKEVARQLKERDA
Sbjct: 181 QGRIINVSSVVHCWVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDA 240

Query: 241 NVTINAVHPGIVKTAIIKAHRGFITGMNYSLFFMASKLLKSIPQGAATTCYVALSPQTQR 300
           NVTINAVHPGIVKTAIIKAHRGFIT    SLFFMASKLLKSIPQGAATTCYVALSPQTQR
Sbjct: 241 NVTINAVHPGIVKTAIIKAHRGFITD---SLFFMASKLLKSIPQGAATTCYVALSPQTQR 300

Query: 301 KTGRYFADCNETKCSSLANDELAARTLWMQTRALILRELGEPV 344
           KTGRYFADCNETKCSSLANDELAARTLWMQTRALILRELGEPV
Sbjct: 301 KTGRYFADCNETKCSSLANDELAARTLWMQTRALILRELGEPV 340

[Close]

BLAST of CmaCh13G008710 vs. NCBI nr
Match: XP_022924073.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 607.4 bits (1565), Expect = 7.4e-170
Identity = 318/343 (92.71%), Postives = 322/343 (93.88%), Query Frame = 0


Query: 1   MWKRESERLNGRVMKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGI 60
           MWKRESERL GRVMKETLRYLVG+AGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGI
Sbjct: 1   MWKRESERLKGRVMKETLRYLVGMAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGI 60

Query: 61  GEETARVLAKRGVRIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCME 120
           GEETARVLAKRGVRIVMAARDMKKAAKVRERIKR GPEAEIL+SEIDLSSLASVRRFCME
Sbjct: 61  GEETARVLAKRGVRIVMAARDMKKAAKVRERIKRGGPEAEILMSEIDLSSLASVRRFCME 120

Query: 121 FAALGLPLNILINNGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGI 180
           FAALGLPLNILINNGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGI
Sbjct: 121 FAALGLPLNILINNGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGI 180

Query: 181 QGRIINVSSVVHCWVNSNPWSENRTKPCLGYNGTRAYAQSKLACILHAKEVARQLKERDA 240
           QGRIINVSSVVHCWV  +            YNGTRAYAQSKLACILHAKEVARQLKERDA
Sbjct: 181 QGRIINVSSVVHCWVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDA 240

Query: 241 NVTINAVHPGIVKTAIIKAHRGFITGMNYSLFFMASKLLKSIPQGAATTCYVALSPQTQR 300
           NVTINAVHPGIVKTAIIKAHRGFIT    SLFFMASKLLKSIPQGAATTCYVALSPQT R
Sbjct: 241 NVTINAVHPGIVKTAIIKAHRGFITD---SLFFMASKLLKSIPQGAATTCYVALSPQTHR 300

Query: 301 KTGRYFADCNETKCSSLANDELAARTLWMQTRALILRELGEPV 344
           KTGRYFADCNETKCSSLANDELAARTLW+QTRALILRELGEPV
Sbjct: 301 KTGRYFADCNETKCSSLANDELAARTLWIQTRALILRELGEPV 340

[Close]

BLAST of CmaCh13G008710 vs. NCBI nr
Match: XP_023519347.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 577.4 bits (1487), Expect = 8.2e-161
Identity = 301/330 (91.21%), Postives = 310/330 (93.94%), Query Frame = 0


Query: 14  MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73
           MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRG+
Sbjct: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGM 60

Query: 74  RIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 133
           RIVMAARDMKKAAKV+ERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN
Sbjct: 61  RIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 120

Query: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193
           NGGVFSPNLEYSDDKLELTFATNYLGHY++TELLLDKLIETS+RTGIQGRIINVSSVVHC
Sbjct: 121 NGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHC 180

Query: 194 WVNSNPWSENRTKPCLGYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253
           WV  +            YNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK
Sbjct: 181 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240

Query: 254 TAIIKAHRGFITGMNYSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETK 313
           TAI+KAHRGFIT    SLFFMASKLLKSIPQGAATTCYVALSPQT+RKTGRYFADCNETK
Sbjct: 241 TAILKAHRGFITD---SLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETK 300

Query: 314 CSSLANDELAARTLWMQTRALILRELGEPV 344
           CSSLANDELAAR LW+QTRALILREL EPV
Sbjct: 301 CSSLANDELAARKLWIQTRALILRELDEPV 327

[Close]

BLAST of CmaCh13G008710 vs. NCBI nr
Match: XP_038895224.1 (short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 552.4 bits (1422), Expect = 2.8e-153
Identity = 291/329 (88.45%), Postives = 302/329 (91.79%), Query Frame = 0


Query: 14  MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73
           MKETLRYLVGIAGPSGYGSK+SAE+VTE  Q SSP TAIITGATSGIGEETARVLAKRGV
Sbjct: 1   MKETLRYLVGIAGPSGYGSKASAEKVTESFQFSSPFTAIITGATSGIGEETARVLAKRGV 60

Query: 74  RIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 133
           RIVMAARDMKKAAKV+ERIKRE PEAEILVSEIDLSSLASVRRFC+EFAALGLPLNILIN
Sbjct: 61  RIVMAARDMKKAAKVKERIKRESPEAEILVSEIDLSSLASVRRFCVEFAALGLPLNILIN 120

Query: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193
           NGGVFSPNLEYS+DK ELTFATNYLGHYLLTELLLDKLIETS+RTGIQGRIINVSSVVH 
Sbjct: 121 NGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHY 180

Query: 194 WVNSNPWSENRTKPCLGYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253
           WV               YNGTRAYAQSKLACILHAKEVAR+LKE+DANVTINAVHPGIVK
Sbjct: 181 WVKREDLCFIHLLNPNKYNGTRAYAQSKLACILHAKEVARKLKEQDANVTINAVHPGIVK 240

Query: 254 TAIIKAHRGFITGMNYSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETK 313
           TAII+AHRGFIT    SLFFMASKLLKSIPQGAATTCYVALSPQTQRKTG+YFADCN+TK
Sbjct: 241 TAIIRAHRGFITD---SLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGKYFADCNKTK 300

Query: 314 CSSLANDELAARTLWMQTRALILRELGEP 343
           CSSLANDEL ARTLWMQTRALILRELGEP
Sbjct: 301 CSSLANDELTARTLWMQTRALILRELGEP 326

[Close]

BLAST of CmaCh13G008710 vs. NCBI nr
Match: KAG7019803.1 (Short-chain dehydrogenase TIC 32, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 542.3 bits (1396), Expect = 2.9e-150
Identity = 295/370 (79.73%), Postives = 304/370 (82.16%), Query Frame = 0


Query: 14  MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73
           MKETLRYLVG+AGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV
Sbjct: 1   MKETLRYLVGMAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 60

Query: 74  RIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 133
           RIVMAARDMKKAAKVRERIKR GPEAEIL+SEIDLSSLASVRRFCMEFAALGLPLNILIN
Sbjct: 61  RIVMAARDMKKAAKVRERIKRGGPEAEILMSEIDLSSLASVRRFCMEFAALGLPLNILIN 120

Query: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193
           NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC
Sbjct: 121 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 180

Query: 194 WVNSNPWSENRTKPCLGYNGTRAYAQSKLACILHAKEVARQLK----------------- 253
           WV  +            YNGTRAYAQSKLACILHAKEVARQLK                 
Sbjct: 181 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKVILLLQLLRFLQTVDKT 240

Query: 254 -----------ERDANVTINAVHPGIVKTA------------IIKAHRGFITGMNYSLFF 313
                      ERDANVTINAVHPGI  +A            ++  H   +T +  SLFF
Sbjct: 241 VHILKFQPFLQERDANVTINAVHPGIPSSASSTSTKRKMYKRLLIYHNYILTSLADSLFF 300

Query: 314 MASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETKCSSLANDELAARTLWMQTRA 344
           MASKLLKSIPQGAATTCYVALSPQT RKTGRYFADCNETKCSSLANDELAARTLW+QTRA
Sbjct: 301 MASKLLKSIPQGAATTCYVALSPQTHRKTGRYFADCNETKCSSLANDELAARTLWIQTRA 360

[Close]

BLAST of CmaCh13G008710 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 423.3 bits (1087), Expect = 1.8e-118
Identity = 225/331 (67.98%), Postives = 269/331 (81.27%), Query Frame = 0


Query: 14  MKETLRYLVGIAGPSGYGSKSSAEQVTEH--LQCSSPLTAIITGATSGIGEETARVLAKR 73
           MK TLRYL GIAGP+G+GS+S+AEQVT+H    CS  LTAIITG TSGIG ETARVLAKR
Sbjct: 1   MKGTLRYLAGIAGPNGFGSRSTAEQVTQHSFFPCSH-LTAIITGGTSGIGAETARVLAKR 60

Query: 74  GVRIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNIL 133
           GVR+VMA RDMKKA  V+ERI RE PEA+I++ EIDLSSL+SV RFC +F +  LPLNIL
Sbjct: 61  GVRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNIL 120

Query: 134 INNGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVV 193
           INN GVFSPNLE+S++K+ELTFATN+LGHYLLTE+L++K+I+T+ ++GI+GRIIN+SSV+
Sbjct: 121 INNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVI 180

Query: 194 HCWVNSNPWS-ENRTKPCLGYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPG 253
           H WV  + +S      P   YNGTRAYAQSKLA ILHAK +++QLK+R+ANVTINAVHPG
Sbjct: 181 HNWVKPDCFSFPKLLHPISRYNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHPG 240

Query: 254 IVKTAIIKAHRGFITGMNYSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCN 313
           IVKT II+AH+G  T    SLF +ASKLLKSI QGAATTCYVALS +T+  +G+YFADCN
Sbjct: 241 IVKTGIIRAHKGLFTD---SLFLIASKLLKSISQGAATTCYVALSNETKGLSGKYFADCN 300

Query: 314 ETKCSSLANDELAARTLWMQTRALILRELGE 342
           ET CS LANDE  A  L  Q+RALI   L E
Sbjct: 301 ETNCSDLANDEYVALKLCTQSRALIHDHLHE 327

[Close]

BLAST of CmaCh13G008710 vs. TAIR 10
Match: AT5G50130.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 355.1 bits (910), Expect = 6.1e-98
Identity = 188/274 (68.61%), Postives = 227/274 (82.85%), Query Frame = 0


Query: 14  MKETLRYLVGIAGPSGYGSKSSAEQVTEH--LQCSSPLTAIITGATSGIGEETARVLAKR 73
           MK TLRYL GIAGP+G+GS+S+AEQVT+H    CS  LTAIITG TSGIG ETARVLAKR
Sbjct: 1   MKGTLRYLAGIAGPNGFGSRSTAEQVTQHSFFPCSH-LTAIITGGTSGIGAETARVLAKR 60

Query: 74  GVRIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNIL 133
           GVR+VMA RDMKKA  V+ERI RE PEA+I++ EIDLSSL+SV RFC +F +  LPLNIL
Sbjct: 61  GVRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNIL 120

Query: 134 INNGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVV 193
           INN GVFSPNLE+S++K+ELTFATN+LGHYLLTE+L++K+I+T+ ++GI+GRIIN+SSV+
Sbjct: 121 INNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVI 180

Query: 194 HCWVNSNPWS-ENRTKPCLGYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPG 253
           H WV  + +S      P   YNGTRAYAQSKLA ILHAK +++QLK+R+ANVTINAVHPG
Sbjct: 181 HNWVKPDCFSFPKLLHPISRYNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHPG 240

Query: 254 IVKTAIIKAHRGFITGMNYSLFFMASKLLKSIPQ 285
           IVKT II+AH+G  T    SLF +ASKLLKSI Q
Sbjct: 241 IVKTGIIRAHKGLFTD---SLFLIASKLLKSISQ 270

[Close]

BLAST of CmaCh13G008710 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 327.4 bits (838), Expect = 1.4e-89
Identity = 174/324 (53.70%), Postives = 233/324 (71.91%), Query Frame = 0


Query: 14  MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73
           M ET+++L+G  GPSG+GS+S+A+ VT +    S LTAIITGATSGIG ETARVLAKRG 
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVTCNSDLRS-LTAIITGATSGIGAETARVLAKRGA 60

Query: 74  RIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 133
           R+V+ AR +K A + + RI  E P+AEI+V  +DLSSL SVRRF  +F +L LPLNILIN
Sbjct: 61  RLVLPARSVKTAEETKARILSEFPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNILIN 120

Query: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193
           N G ++     S+D +E+TFATNYLGH+LLT+LLL K+IET+ +TG+QGRI+NV+SVVH 
Sbjct: 121 NAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSVVHS 180

Query: 194 WVNSN--PWSENRTKPCLGYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGI 253
           W + +   +  + ++    Y+ TRAYA SKLA +LH  E++R L + DANVT N VHPGI
Sbjct: 181 WFSGDMLQYLADISRNNRNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCVHPGI 240

Query: 254 VKTAIIKAHRGFITGMNYSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNE 313
           VKT + +   G +T +   +FF+ SKLLKS+PQ AATTCYVA SP+ +   G+YF+DCNE
Sbjct: 241 VKTRLTRDREGVVTDL---VFFLTSKLLKSVPQAAATTCYVATSPRLRNVCGKYFSDCNE 300

Query: 314 TKCSSLANDELAARTLWMQTRALI 336
            + S   +  L A+ LW  +  L+
Sbjct: 301 ARSSKSGSCNLKAQRLWTASDLLV 320

[Close]

BLAST of CmaCh13G008710 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 323.2 bits (827), Expect = 2.6e-88
Identity = 168/324 (51.85%), Postives = 233/324 (71.91%), Query Frame = 0


Query: 14  MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73
           M ET +YL+G AG SG+GSKS+AE+VTE+    S +TA+ITGATSGIG ETARVLAKRG 
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTENCDLRS-ITAVITGATSGIGAETARVLAKRGA 60

Query: 74  RIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 133
           R++  AR++K A + +ERI  E PE EI+V ++DLSS+ASVR F  +F +L LPLN+LIN
Sbjct: 61  RLIFPARNVKAAEEAKERIVSEFPETEIVVMKLDLSSIASVRNFVADFESLDLPLNLLIN 120

Query: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193
           N G  +     S+D +E+TFATNYLGH+LLT LLL+K+I+T+  TG+QGRI+NV+S +H 
Sbjct: 121 NAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSGIHG 180

Query: 194 WVNSNPWSENR--TKPCLGYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGI 253
           W + +     R  ++P   ++ TRAYA SKLA +LH KE++ +L++  ANVT+N VHPG+
Sbjct: 181 WFSGDLIEYLRLISQPKCQFDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCVHPGV 240

Query: 254 VKTAIIKAHRGFITGMNYSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNE 313
           V+T + +   G +T +   +FF+ASKL+K++PQ AATTCYVA +P+    +G+YF DCNE
Sbjct: 241 VRTRLTRDREGLLTDL---VFFLASKLVKTVPQAAATTCYVATNPRLVNVSGKYFTDCNE 300

Query: 314 TKCSSLANDELAARTLWMQTRALI 336
           T  S L  +   A  LW  +  L+
Sbjct: 301 TTPSGLGTNSSEATKLWAASEILV 320

[Close]

BLAST of CmaCh13G008710 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 280.4 bits (716), Expect = 1.9e-75
Identity = 151/308 (49.03%), Postives = 205/308 (66.56%), Query Frame = 0


Query: 21  LVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGVRIVMAAR 80
           + G  GPSG+GS S+AE+VT+ +  ++ LTAIITG T GIG ETARVL+KRG  +V+ AR
Sbjct: 7   ITGRRGPSGFGSASTAEEVTQGIDATN-LTAIITGGTGGIGMETARVLSKRGAHVVIGAR 66

Query: 81  DMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILINNGGVFSP 140
           +M  A   +  I R+   A + + ++DLSS+ S++ F  EF AL LPLN+LINN GV   
Sbjct: 67  NMGAAENAKTEILRQNANARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLINNAGVMFC 126

Query: 141 NLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHCWVNSNPW 200
             + S+D +EL FATN++GH+LLT LLLD +  T++ +G++GRI+NVSSV H +      
Sbjct: 127 PYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHIYTYQEGI 186

Query: 201 SENRTKPCLGYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVKTAIIKAH 260
             +       Y+  RAY QSKLA ILHA E++RQL+E   N+T N+VHPG++ T + + H
Sbjct: 187 QFDSINDICSYSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHPGLILTNLFQ-H 246

Query: 261 RGFITGMNYSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETKCSSLAND 320
              +  M +  FF +  L K+IPQGAATTCYVAL P  +  TG+YFADCNE   S LA D
Sbjct: 247 TALL--MRFLKFF-SFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNEVTPSKLARD 306

Query: 321 ELAARTLW 329
           E  A+ LW
Sbjct: 307 ETLAQKLW 309

The following BLAST results are available for this feature:

BLAST of CmaCh13G008710 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A078IS66	8.4e-68	47.08	Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A0A078ISJ6	1.1e-67	47.08	Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A2RVM0	1.4e-67	45.65	Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q6RVV4	2.8e-63	43.89	Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3...	[more]
Q9HBH5	2.6e-40	37.88	Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1	[more]

BLAST of CmaCh13G008710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KGJ5	2.0e-173	94.46	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita maxima OX=3661...	[more]
A0A6J1EBB9	3.6e-170	92.71	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita moschata OX=36...	[more]
A0A6J1C5L3	3.4e-144	82.93	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3...	[more]
A0A6J1BYZ0	4.1e-126	71.51	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3...	[more]
A0A2P5FFY3	1.0e-124	70.69	Short-chain dehydrogenase/reductase OS=Trema orientale OX=63057 GN=TorRG33x02_07...	[more]

BLAST of CmaCh13G008710 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023000696.1	4.2e-173	94.46	short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita maxima]	[more]
XP_022924073.1	7.4e-170	92.71	short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita moschata]	[more]
XP_023519347.1	8.2e-161	91.21	short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo...	[more]
XP_038895224.1	2.8e-153	88.45	short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X2 [Benincasa his...	[more]
KAG7019803.1	2.9e-150	79.73	Short-chain dehydrogenase TIC 32, chloroplastic, partial [Cucurbita argyrosperma...	[more]

BLAST of CmaCh13G008710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50130.1	1.8e-118	67.98	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.2	6.1e-98	68.61	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G64590.1	1.4e-89	53.70	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24050.1	2.6e-88	51.85	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G02540.1	1.9e-75	49.03	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 240..257 score: 33.16 coord: 176..192 score: 32.7 coord: 217..236 score: 27.97 coord: 50..67 score: 44.95 coord: 126..137 score: 39.38
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 217..236 score: 26.08 coord: 126..137 score: 38.63 coord: 182..190 score: 61.15
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 50..191 e-value: 2.6E-26 score: 92.3
None	No IPR available	GENE3D	3.40.50.720		coord: 38..338 e-value: 1.7E-70 score: 239.4
None	No IPR available	PANTHER	PTHR24320	RETINOL DEHYDROGENASE	coord: 17..338
None	No IPR available	PANTHER	PTHR24320:SF198	NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN	coord: 17..338
None	No IPR available	CDD	cd05327	retinol-DH_like_SDR_c_like	coord: 50..328 e-value: 3.20122E-87 score: 261.775
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 50..313

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh13G008710.1	CmaCh13G008710.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity