CmaCh13G008570 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh13G008570
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationCma_Chr13: 7058703 .. 7060371 (-)
RNA-Seq ExpressionCmaCh13G008570
SyntenyCmaCh13G008570
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCACAGGCAGTCGCCTGCGCTGTTCCAATCCTCGAAGCCCTTAAAGCTTACAAGTGGGGCTTCACAAATATCCAATCTTCTTCGTTTTTCTGCTAAGATAAATCCTCCTCCATGGTCTCTTTCTCTCTCTTCTTCTCTGTTCTATCTCGTTCTCTTTCTAACTCGTTTTTTAATGTGTTGCAGGCCGCTCGAACACATGATGGGCTCGTGTATCAAGAGGTTAGCCCGCCCGCCCTTAATATTGTCCAGTTTGAGTATAAGTTCCAGTAACTTTGGCTTGAATATCTTAGATAGACAGTATTCTTTACTTATAAATCTATGAATCGTTCTCTAAATTAGTTAATATCCAACTCCGTGTTTTGCATTGTAGGGGTTTATAATGAACTCATGTGGGTTGAAGCTTTTCACGTGCGAATGGCTTCCAAAGGATAAGGAACCAAAGGGCTTGATCTTTATCTGCCATGGGTACGCGATGGAATGCAGCATCACCATGAACAGTAAACACCCTTCCACCACCTGAAACATTATATTCCTAAGATCAAATTATAATTGATTTGGTTTATAGGCTCTGCAATTCGGCTTGCAAGGGCAGGGTTTGCGGTTTATGGGATTGATTACGAAGGCCATGGGAAATCAGATGGGCTGCAAGGCTATATTACAAGCTTTGATCATGTGGTGGAGGATTGCTCCAATCACTTCACAGCCATTTCTGGTATCTTATCTTCCCAATGCAAAAGATTCAATTTTTATGGGTTTTAAGTGAAAATTTTTACATGGGTCTTCGTTAAAAACTTTGTTTTTTAATACCCAGAAAGGAAAGAGAACATGGGCAAAAGAAGGTATCTATTAGGGGAGTCAATGGGAGGGGCAGTGGCTCTGTTATTGCACAGAAAGAAGCCAGATTATTGGGATGGTGCTGTCTTGGTTGCACCAATGTGTAAAGTATGTCCATTTTTTGGCTCTGTTTTTGAATGAGTTTAAAATTTGAAGCTCAAAAAATTCCCCTGTTTTTGGCAGATTGCAGAGGATGTTAAACCAAAGCCAGTTGTTGTAAATATACTGACAAAGCTTTGTAAAGTGATACCCACATGGAAGATAATTCCAACCCAAGATATCATTGATGTGGCTTTCAAGGTTCCTGAGATTAGACATCAGGTAATAATTATTAATCTCCTCTGTTTTTACAATATAAAACTGTTTATATTAATGATAATAAAATTAACAGGTCAGAAATAATCCTTATTGTTTCAAAGGGAGGCCTCGTTTGAACACTGGAAATGAACTTCTAAGGATCAGTTTAGATCTCGAGAAACGACTCGAAGAGGTTTGCTATTTCTTCGCCGTTTGAATGAATTAGTCTTTTTCGATTTTTTGAGTTCAATGGTAGTTTATAGGTTTCGTTACCGTTCATAATCCTCCACGGGGAGGCGGATCGAGTGACCGATATATCGGTGAGCAAGCAACTCCTTGAGAAGGCGTCGAGCTGGGACAAGAGCTTGAAGACATATCCAGAGATGTGGCATGGATTGCTGTATGGAGAGACAGTTGAAAACATTGATGTTGTGTTTGCTGACATAATTGGTTGGTTGGAAGAAAGATGTGGTTTGGGAAATTCAAGGATTGAGAAACAGCTCAAGACAGAATCTGATGATCTTCAAACCAAATAA

mRNA sequence

ATGCACAGGCAGTCGCCTGCGCTGTTCCAATCCTCGAAGCCCTTAAAGCTTACAAGTGGGGCTTCACAAATATCCAATCTTCTTCGTTTTTCTGCTAAGATAAATCCTCCTCCATGGTCTCTTTCTCTCTCTTCTTCTCTGTTCTATCTCGATAAGGAACCAAAGGGCTTGATCTTTATCTGCCATGGGTACGCGATGGAATGCAGCATCACCATGAACAGCTCTGCAATTCGGCTTGCAAGGGCAGGGTTTGCGGTTTATGGGATTGATTACGAAGGCCATGGGAAATCAGATGGGCTGCAAGGCTATATTACAAGCTTTGATCATGTGGTGGAGGATTGCTCCAATCACTTCACAGCCATTTCTGAAAGGAAAGAGAACATGGGCAAAAGAAGGTATCTATTAGGGGAGTCAATGGGAGGGGCAGTGGCTCTGTTATTGCACAGAAAGAAGCCAGATTATTGGGATGGTGCTGTCTTGGTTGCACCAATGTGTAAAATTGCAGAGGATGTTAAACCAAAGCCAGTTGTTGTAAATATACTGACAAAGCTTTGTAAAGTGATACCCACATGGAAGATAATTCCAACCCAAGATATCATTGATGTGGCTTTCAAGGTTCCTGAGATTAGACATCAGGTCAGAAATAATCCTTATTGTTTCAAAGGGAGGCCTCGTTTGAACACTGGAAATGAACTTCTAAGGATCAGTTTAGATCTCGAGAAACGACTCGAAGAGGTTTCGTTACCGTTCATAATCCTCCACGGGGAGGCGGATCGAGTGACCGATATATCGGTGAGCAAGCAACTCCTTGAGAAGGCGTCGAGCTGGGACAAGAGCTTGAAGACATATCCAGAGATGTGGCATGGATTGCTGTATGGAGAGACAGTTGAAAACATTGATGTTGTGTTTGCTGACATAATTGGTTGGTTGGAAGAAAGATGTGGTTTGGGAAATTCAAGGATTGAGAAACAGCTCAAGACAGAATCTGATGATCTTCAAACCAAATAA

Coding sequence (CDS)

ATGCACAGGCAGTCGCCTGCGCTGTTCCAATCCTCGAAGCCCTTAAAGCTTACAAGTGGGGCTTCACAAATATCCAATCTTCTTCGTTTTTCTGCTAAGATAAATCCTCCTCCATGGTCTCTTTCTCTCTCTTCTTCTCTGTTCTATCTCGATAAGGAACCAAAGGGCTTGATCTTTATCTGCCATGGGTACGCGATGGAATGCAGCATCACCATGAACAGCTCTGCAATTCGGCTTGCAAGGGCAGGGTTTGCGGTTTATGGGATTGATTACGAAGGCCATGGGAAATCAGATGGGCTGCAAGGCTATATTACAAGCTTTGATCATGTGGTGGAGGATTGCTCCAATCACTTCACAGCCATTTCTGAAAGGAAAGAGAACATGGGCAAAAGAAGGTATCTATTAGGGGAGTCAATGGGAGGGGCAGTGGCTCTGTTATTGCACAGAAAGAAGCCAGATTATTGGGATGGTGCTGTCTTGGTTGCACCAATGTGTAAAATTGCAGAGGATGTTAAACCAAAGCCAGTTGTTGTAAATATACTGACAAAGCTTTGTAAAGTGATACCCACATGGAAGATAATTCCAACCCAAGATATCATTGATGTGGCTTTCAAGGTTCCTGAGATTAGACATCAGGTCAGAAATAATCCTTATTGTTTCAAAGGGAGGCCTCGTTTGAACACTGGAAATGAACTTCTAAGGATCAGTTTAGATCTCGAGAAACGACTCGAAGAGGTTTCGTTACCGTTCATAATCCTCCACGGGGAGGCGGATCGAGTGACCGATATATCGGTGAGCAAGCAACTCCTTGAGAAGGCGTCGAGCTGGGACAAGAGCTTGAAGACATATCCAGAGATGTGGCATGGATTGCTGTATGGAGAGACAGTTGAAAACATTGATGTTGTGTTTGCTGACATAATTGGTTGGTTGGAAGAAAGATGTGGTTTGGGAAATTCAAGGATTGAGAAACAGCTCAAGACAGAATCTGATGATCTTCAAACCAAATAA

Protein sequence

MHRQSPALFQSSKPLKLTSGASQISNLLRFSAKINPPPWSLSLSSSLFYLDKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHVVEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAEDVKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGLLYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK
Homology
BLAST of CmaCh13G008570 vs. ExPASy Swiss-Prot
Match: Q9C942 (Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 3.3e-40
Identity = 93/271 (34.32%), Postives = 144/271 (53.14%), Query Frame = 0

Query: 44  SSSLFYLDKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGY 103
           + S   LD E KG +++ HGY  + S       +  +  G+AV+  D  GHG+SDG++ Y
Sbjct: 53  TQSFLPLDGEIKGTVYMSHGYGSDTSWMFQKICMSFSSWGYAVFAADLLGHGRSDGIRCY 112

Query: 104 ITSFDHVVEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLH-RKKPDYWDGAVLVA 163
           +   + V       F  +           +L GESMGG V LL++ + +P+ W G +  A
Sbjct: 113 MGDMEKVAATSLAFFKHVRCSDPYKDLPAFLFGESMGGLVTLLMYFQSEPETWTGLMFSA 172

Query: 164 PMCKIAEDVKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKG 223
           P+  I ED+KP    +     L  +  TW  +P   ++  A K PE    + +NP  + G
Sbjct: 173 PLFVIPEDMKPSKAHLFAYGLLFGLADTWAAMPDNKMVGKAIKDPEKLKIIASNPQRYTG 232

Query: 224 RPRLNTGNELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKT 283
           +PR+ T  ELLR +  +++   +V++P    HG AD VT  + SK L EKASS DK+LK 
Sbjct: 233 KPRVGTMRELLRKTQYVQENFGKVTIPVFTAHGTADGVTCPTSSKLLYEKASSADKTLKI 292

Query: 284 YPEMWHGLLYGETVENIDVVFADIIGWLEER 314
           Y  M+H L+ GE  EN ++V  D+  W++E+
Sbjct: 293 YEGMYHSLIQGEPDENAEIVLKDMREWIDEK 323

BLAST of CmaCh13G008570 vs. ExPASy Swiss-Prot
Match: O07427 (Monoacylglycerol lipase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv0183 PE=1 SV=2)

HSP 1 Score: 114.8 bits (286), Expect = 1.9e-24
Identity = 80/264 (30.30%), Postives = 125/264 (47.35%), Query Frame = 0

Query: 51  DKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 110
           D  P+ ++ + HG   E +   +  A RL  AG   Y +D+ GHG+S G +  +      
Sbjct: 26  DTAPQAVVVLAHGLG-EHARRYDHVAQRLGAAGLVTYALDHRGHGRSGGKRVLVRDISEY 85

Query: 111 VEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 170
             D       I+ R E  G +R +LG SMGG +      ++PD +D  VL AP     + 
Sbjct: 86  TADFDT-LVGIATR-EYPGCKRIVLGHSMGGGIVFAYGVERPDNYDLMVLSAPAVAAQDL 145

Query: 171 VKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAF-KVPEIRHQVRNNPYCFKGRPRLNTG 230
           V P   VV +  KL  V+     +P Q++   A  + PE+      +P    GR     G
Sbjct: 146 VSP---VVAVAAKLLGVVVPG--LPVQELDFTAISRDPEVVQAYNTDPLVHHGRVPAGIG 205

Query: 231 NELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHG 290
             LL++   + +R   ++ P ++LHG  DR+  I  S++L+E   S D  LK YP ++H 
Sbjct: 206 RALLQVGETMPRRAPALTAPLLVLHGTDDRLIPIEGSRRLVECVGSADVQLKEYPGLYHE 265

Query: 291 LLYGETVENIDVVFADIIGWLEER 314
            ++ E   N   V  D++ WL ER
Sbjct: 266 -VFNEPERN--QVLDDVVAWLTER 278

BLAST of CmaCh13G008570 vs. ExPASy Swiss-Prot
Match: A0QNZ7 (Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=MSMEG_0220 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.2e-21
Identity = 68/261 (26.05%), Postives = 125/261 (47.89%), Query Frame = 0

Query: 51  DKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 110
           D +P+G++ + HGYA E +   +  A R   AG  VY +D+ GHG+S G + ++      
Sbjct: 27  DTDPRGVVVLAHGYA-EHAGRYHHVAQRFGAAGLLVYALDHRGHGRSGGKRVHLRDLSEF 86

Query: 111 VEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 170
           VED        +     +   R +LG SMGG +      + P  +   VL  P     + 
Sbjct: 87  VEDFRTLVGIAANDHPTL--PRIVLGHSMGGGIVFAYGARYPGEYSAMVLSGPAVNAHDG 146

Query: 171 VKPKPVVVNILTKLCKVIPTWKIIPTQDI-IDVAFKVPEIRHQVRNNPYCFKGRPRLNTG 230
           V   PV+V +   L K+ P    IP +++  D   + PE+    + +P    G+      
Sbjct: 147 V--SPVLVAVAKVLGKLAPG---IPVENLDADAVSRDPEVVAAYKADPMVHHGKLPAGIA 206

Query: 231 NELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHG 290
             L+ +   + +R   ++ P +++HG+ DR+  ++ S+ L+++ +S D  LK YP ++H 
Sbjct: 207 RALIGLGQSMPQRAAALTAPLLVVHGDKDRLIPVAGSRLLVDRVASEDVHLKVYPGLYHE 266

Query: 291 LLYGETVENIDVVFADIIGWL 311
            ++ E  + +  V  D+  W+
Sbjct: 267 -VFNEPEQKL--VLDDVTSWI 276

BLAST of CmaCh13G008570 vs. ExPASy Swiss-Prot
Match: Q99685 (Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2)

HSP 1 Score: 105.1 bits (261), Expect = 1.5e-21
Identity = 80/260 (30.77%), Postives = 132/260 (50.77%), Query Frame = 0

Query: 54  PKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHVVED 113
           PK LIF+ HG A E S      A  L      V+  D+ GHG+S+G +  ++ F   V D
Sbjct: 41  PKALIFVSHG-AGEHSGRYEELARMLMGLDLLVFAHDHVGHGQSEGERMVVSDFHVFVRD 100

Query: 114 CSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAEDVKP 173
              H    S +K+  G   +LLG SMGGA+A+L   ++P ++ G VL++P+  +A     
Sbjct: 101 VLQHVD--SMQKDYPGLPVFLLGHSMGGAIAILTAAERPGHFAGMVLISPLV-LANPESA 160

Query: 174 KPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGNELL 233
               V     L  V+P   + P    + ++    E+     ++P   +   ++  G +LL
Sbjct: 161 TTFKVLAAKVLNLVLPNLSLGPIDSSV-LSRNKTEV-DIYNSDPLICRAGLKVCFGIQLL 220

Query: 234 RISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGLLYG 293
                +E+ L ++++PF++L G ADR+ D   +  L+E A S DK+LK Y   +H +L+ 
Sbjct: 221 NAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELAKSQDKTLKIYEGAYH-VLHK 280

Query: 294 ETVENIDVVFADIIGWLEER 314
           E  E  + VF +I  W+ +R
Sbjct: 281 ELPEVTNSVFHEINMWVSQR 293

BLAST of CmaCh13G008570 vs. ExPASy Swiss-Prot
Match: O35678 (Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 7.6e-21
Identity = 71/260 (27.31%), Postives = 128/260 (49.23%), Query Frame = 0

Query: 54  PKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHVVED 113
           PK LIF+ HG    C    +  A  L      V+  D+ GHG+S+G +  ++ F   V D
Sbjct: 41  PKALIFVSHGAGEHCG-RYDELAHMLKGLDMLVFAHDHVGHGQSEGERMVVSDFQVFVRD 100

Query: 114 CSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAEDVKP 173
              H   I  +K+      +LLG SMGGA+++L+  ++P Y+ G VL++P+  +  + + 
Sbjct: 101 VLQHVDTI--QKDYPDVPIFLLGHSMGGAISILVAAERPTYFSGMVLISPL--VLANPES 160

Query: 174 KPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGNELL 233
              +  +  KL   +     +   D   ++    E+     ++P   +   ++  G +LL
Sbjct: 161 ASTLKVLAAKLLNFVLPNMTLGRIDSSVLSRNKSEV-DLYNSDPLVCRAGLKVCFGIQLL 220

Query: 234 RISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGLLYG 293
                +E+ +  ++LPF++L G ADR+ D   +  L+E + S DK+LK Y   +H +L+ 
Sbjct: 221 NAVARVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSRSQDKTLKMYEGAYH-VLHR 280

Query: 294 ETVENIDVVFADIIGWLEER 314
           E  E  + V  ++  W+  R
Sbjct: 281 ELPEVTNSVLHEVNSWVSHR 293

BLAST of CmaCh13G008570 vs. ExPASy TrEMBL
Match: A0A6J1KJY0 (caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111495282 PE=4 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.4e-163
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0

Query: 51  DKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 110
           DKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV
Sbjct: 17  DKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 76

Query: 111 VEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 170
           VEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED
Sbjct: 77  VEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 136

Query: 171 VKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN 230
           VKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN
Sbjct: 137 VKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN 196

Query: 231 ELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL 290
           ELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL
Sbjct: 197 ELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL 256

Query: 291 LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK 336
           LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK
Sbjct: 257 LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK 301

BLAST of CmaCh13G008570 vs. ExPASy TrEMBL
Match: A0A6J1ECU4 (caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111431373 PE=4 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 3.0e-161
Identity = 280/285 (98.25%), Postives = 282/285 (98.95%), Query Frame = 0

Query: 51  DKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 110
           DKEPKGLIFICHGYAMECSITMNS+AIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV
Sbjct: 18  DKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 77

Query: 111 VEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 170
           VEDCSNHFTA SERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED
Sbjct: 78  VEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 137

Query: 171 VKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN 230
           VKPKP+VVNIL KLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN
Sbjct: 138 VKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN 197

Query: 231 ELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL 290
           ELLRISLDLEKRLEEVSLPFIILHGE DRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL
Sbjct: 198 ELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL 257

Query: 291 LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK 336
           LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK
Sbjct: 258 LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK 302

BLAST of CmaCh13G008570 vs. ExPASy TrEMBL
Match: A0A6J1GSV7 (caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111457223 PE=4 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 5.4e-147
Identity = 251/285 (88.07%), Postives = 272/285 (95.44%), Query Frame = 0

Query: 51  DKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 110
           ++EPKGLIFICHGYAMECSITMNS+A+RLA+AGFAV+GIDYEGHGKSDGLQGYITSFDHV
Sbjct: 33  NQEPKGLIFICHGYAMECSITMNSTAVRLAKAGFAVHGIDYEGHGKSDGLQGYITSFDHV 92

Query: 111 VEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 170
           V+DCSNHFTAISE KEN  K RYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED
Sbjct: 93  VDDCSNHFTAISEMKENKRKMRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 152

Query: 171 VKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN 230
           VKP P+VV+ILTKLC +IPTWKIIPTQDIIDVAFK PEIRHQ+R NPYC+KGRPRLNTG+
Sbjct: 153 VKPSPIVVSILTKLCNLIPTWKIIPTQDIIDVAFKAPEIRHQIRTNPYCYKGRPRLNTGH 212

Query: 231 ELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL 290
           ELL+ISLDLE+RLEEVS+PF+ILHGEADRVTDISVS+QL EKASSWDK LK YPEMWHGL
Sbjct: 213 ELLKISLDLEQRLEEVSIPFMILHGEADRVTDISVSEQLHEKASSWDKRLKKYPEMWHGL 272

Query: 291 LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK 336
           LYGETVENID+VFADIIGWL+ERC LGNSRIE+QLKTE DDLQTK
Sbjct: 273 LYGETVENIDIVFADIIGWLDERCALGNSRIEQQLKTECDDLQTK 317

BLAST of CmaCh13G008570 vs. ExPASy TrEMBL
Match: A0A6J1JZ46 (caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111490153 PE=4 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 2.3e-145
Identity = 249/285 (87.37%), Postives = 271/285 (95.09%), Query Frame = 0

Query: 51  DKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 110
           ++EPKGLIFICHGYAMECSITMNS+A+RLA+AGFAV+GIDYEGHGKSDGLQGYITSFDHV
Sbjct: 33  NQEPKGLIFICHGYAMECSITMNSTAVRLAKAGFAVHGIDYEGHGKSDGLQGYITSFDHV 92

Query: 111 VEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 170
           V+DCSNHFTAISE KEN  K RYLLGESMGGA+ALLLHRKKPDYWDGAVLVAPMCKIAE+
Sbjct: 93  VDDCSNHFTAISEMKENKMKMRYLLGESMGGAMALLLHRKKPDYWDGAVLVAPMCKIAEN 152

Query: 171 VKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN 230
           VKP P+VV+ILTKLC +IPTWKIIPTQDIIDVAFK PEIR Q+R NPYC+KGRPRLNTG+
Sbjct: 153 VKPSPIVVSILTKLCNLIPTWKIIPTQDIIDVAFKAPEIRRQIRTNPYCYKGRPRLNTGH 212

Query: 231 ELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL 290
           ELLRISLDLE+RLEEVS+PF+ILHGEADRVTDISVS+QL  KASSWDKSLK YPEMWHGL
Sbjct: 213 ELLRISLDLEQRLEEVSIPFMILHGEADRVTDISVSEQLHRKASSWDKSLKKYPEMWHGL 272

Query: 291 LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK 336
           LYGETVENID+VFADIIGWL+ERC LGNSRIE+QLKTE DDLQTK
Sbjct: 273 LYGETVENIDIVFADIIGWLDERCALGNSRIEQQLKTECDDLQTK 317

BLAST of CmaCh13G008570 vs. ExPASy TrEMBL
Match: A0A6J1DU62 (caffeoylshikimate esterase-like OS=Momordica charantia OX=3673 GN=LOC111024458 PE=4 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 3.0e-145
Identity = 247/285 (86.67%), Postives = 268/285 (94.04%), Query Frame = 0

Query: 51  DKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 110
           ++EPKGLIFICHGYAMECSITM S+AIRLA+AGFA+YG+DYEGHGKSDGLQGYITSFDHV
Sbjct: 32  NQEPKGLIFICHGYAMECSITMKSTAIRLAKAGFAIYGVDYEGHGKSDGLQGYITSFDHV 91

Query: 111 VEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 170
           V+DCSNHFT+ISERK+N GK RYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIA+D
Sbjct: 92  VDDCSNHFTSISERKQNKGKMRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIADD 151

Query: 171 VKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN 230
           VKP P+VVNILTKLC  IPTWKIIPTQDIIDVAFKVPEIRHQ+R NPYC+KGRPRLNTG+
Sbjct: 152 VKPNPIVVNILTKLCNFIPTWKIIPTQDIIDVAFKVPEIRHQIRTNPYCYKGRPRLNTGH 211

Query: 231 ELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL 290
           +LL+IS DLE+RLEEVS PFIILHGE DRVTDISVS+QL EKASSWDKSLKTYPEMWHGL
Sbjct: 212 QLLKISSDLEQRLEEVSFPFIILHGEVDRVTDISVSEQLYEKASSWDKSLKTYPEMWHGL 271

Query: 291 LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK 336
           LYGET+ENIDVVFA II WLEERC LGNSRIE+QLK+E DD Q K
Sbjct: 272 LYGETLENIDVVFAGIIAWLEERCALGNSRIERQLKSECDDQQAK 316

BLAST of CmaCh13G008570 vs. NCBI nr
Match: XP_023001020.1 (caffeoylshikimate esterase-like [Cucurbita maxima])

HSP 1 Score: 584.7 bits (1506), Expect = 5.0e-163
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0

Query: 51  DKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 110
           DKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV
Sbjct: 17  DKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 76

Query: 111 VEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 170
           VEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED
Sbjct: 77  VEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 136

Query: 171 VKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN 230
           VKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN
Sbjct: 137 VKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN 196

Query: 231 ELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL 290
           ELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL
Sbjct: 197 ELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL 256

Query: 291 LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK 336
           LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK
Sbjct: 257 LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK 301

BLAST of CmaCh13G008570 vs. NCBI nr
Match: XP_023520138.1 (caffeoylshikimate esterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 578.9 bits (1491), Expect = 2.7e-161
Identity = 281/285 (98.60%), Postives = 283/285 (99.30%), Query Frame = 0

Query: 51  DKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 110
           DKEPKGLIFICHGYAMECSITMNS+AIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV
Sbjct: 36  DKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 95

Query: 111 VEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 170
           VEDCSNHFTA SERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED
Sbjct: 96  VEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 155

Query: 171 VKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN 230
           VKPKP+VVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN
Sbjct: 156 VKPKPIVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN 215

Query: 231 ELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL 290
           ELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL
Sbjct: 216 ELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL 275

Query: 291 LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK 336
           LYGETVENIDVVFADIIGWLEERCGLGN RIEKQLKTESDDLQTK
Sbjct: 276 LYGETVENIDVVFADIIGWLEERCGLGNLRIEKQLKTESDDLQTK 320

BLAST of CmaCh13G008570 vs. NCBI nr
Match: KAG7019788.1 (Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 577.8 bits (1488), Expect = 6.1e-161
Identity = 280/285 (98.25%), Postives = 283/285 (99.30%), Query Frame = 0

Query: 51  DKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 110
           DKEPKGLIFICHGYAMECSITMNS+AIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV
Sbjct: 33  DKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 92

Query: 111 VEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 170
           VEDCSNHFTA SERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED
Sbjct: 93  VEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 152

Query: 171 VKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN 230
           VKPKP+VVNIL KLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN
Sbjct: 153 VKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN 212

Query: 231 ELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL 290
           ELLRISLDLEKRLEEVSLPFIILHGEAD+VTDISVSKQLLEKASSWDKSLKTYPEMWHGL
Sbjct: 213 ELLRISLDLEKRLEEVSLPFIILHGEADQVTDISVSKQLLEKASSWDKSLKTYPEMWHGL 272

Query: 291 LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK 336
           LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK
Sbjct: 273 LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK 317

BLAST of CmaCh13G008570 vs. NCBI nr
Match: XP_022923765.1 (caffeoylshikimate esterase-like [Cucurbita moschata])

HSP 1 Score: 577.8 bits (1488), Expect = 6.1e-161
Identity = 280/285 (98.25%), Postives = 282/285 (98.95%), Query Frame = 0

Query: 51  DKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 110
           DKEPKGLIFICHGYAMECSITMNS+AIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV
Sbjct: 18  DKEPKGLIFICHGYAMECSITMNSTAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 77

Query: 111 VEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 170
           VEDCSNHFTA SERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED
Sbjct: 78  VEDCSNHFTATSERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 137

Query: 171 VKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN 230
           VKPKP+VVNIL KLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN
Sbjct: 138 VKPKPIVVNILAKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN 197

Query: 231 ELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL 290
           ELLRISLDLEKRLEEVSLPFIILHGE DRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL
Sbjct: 198 ELLRISLDLEKRLEEVSLPFIILHGEVDRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL 257

Query: 291 LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK 336
           LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK
Sbjct: 258 LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK 302

BLAST of CmaCh13G008570 vs. NCBI nr
Match: XP_038894800.1 (caffeoylshikimate esterase-like [Benincasa hispida])

HSP 1 Score: 533.5 bits (1373), Expect = 1.3e-147
Identity = 255/285 (89.47%), Postives = 270/285 (94.74%), Query Frame = 0

Query: 51  DKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 110
           DKEPKGLIFICHGYAMECSITMNS+AIRLA+AGFA+YGIDYEGHGKSDGLQGYITSFD+V
Sbjct: 33  DKEPKGLIFICHGYAMECSITMNSTAIRLAQAGFAIYGIDYEGHGKSDGLQGYITSFDYV 92

Query: 111 VEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 170
           V+DCSN+FT ISERKEN GK RYLLGESMG AVALLLHRKKPDYWDGAVLVAPMCKIAED
Sbjct: 93  VDDCSNYFTEISERKENKGKMRYLLGESMGAAVALLLHRKKPDYWDGAVLVAPMCKIAED 152

Query: 171 VKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN 230
           VKP P+VVNILTKLC VIPTWKIIPTQDIIDVAFKVPEIRHQ+R NPYC+KGRPRLNTG+
Sbjct: 153 VKPSPIVVNILTKLCNVIPTWKIIPTQDIIDVAFKVPEIRHQIRTNPYCYKGRPRLNTGH 212

Query: 231 ELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL 290
           ELLRISLDLE+RLEEVSLPFIILHGE DRVTD+S S+QL EKASSWDK+LK YPEMWHGL
Sbjct: 213 ELLRISLDLEQRLEEVSLPFIILHGEEDRVTDVSASEQLHEKASSWDKTLKKYPEMWHGL 272

Query: 291 LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK 336
           LYGET ENIDVVFADII WL+ERC LGNSRIEKQLKTE DDLQTK
Sbjct: 273 LYGETDENIDVVFADIIAWLDERCALGNSRIEKQLKTECDDLQTK 317

BLAST of CmaCh13G008570 vs. TAIR 10
Match: AT2G39420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 418.7 bits (1075), Expect = 4.4e-117
Identity = 198/284 (69.72%), Postives = 235/284 (82.75%), Query Frame = 0

Query: 52  KEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHVV 111
           +EPK L+FICHGYAMECSITMNS+A RL +AGFAVYGIDYEGHGKSDGL  Y+ +FDH+V
Sbjct: 33  QEPKALVFICHGYAMECSITMNSTARRLVKAGFAVYGIDYEGHGKSDGLSAYVPNFDHLV 92

Query: 112 EDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAEDV 171
           +D S H+T+I E++EN GK R+LLGESMGGAV LLLHRKKP +WDGAVLVAPMCKIAE++
Sbjct: 93  DDVSTHYTSICEKEENKGKMRFLLGESMGGAVLLLLHRKKPQFWDGAVLVAPMCKIAEEM 152

Query: 172 KPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGNE 231
           KP P+V++IL KL  VIP+WKIIP QDII+ AFK PEIR QVR NPYC+KGRPRL T  E
Sbjct: 153 KPSPLVISILAKLSGVIPSWKIIPGQDIIETAFKQPEIRKQVRENPYCYKGRPRLKTAYE 212

Query: 232 LLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGLL 291
           LLR+S DLEKRL EVSLPFI+LHGE D+VTD +VS+QL E ASS DK+ K YP MWHGLL
Sbjct: 213 LLRVSTDLEKRLNEVSLPFIVLHGEDDKVTDKAVSRQLYEVASSSDKTFKLYPGMWHGLL 272

Query: 292 YGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK 336
           YGET ENI+ VFADIIGWL+++    +   E +LK ++D +  K
Sbjct: 273 YGETPENIETVFADIIGWLDKKVADESGGFESELKRKNDGIPLK 316

BLAST of CmaCh13G008570 vs. TAIR 10
Match: AT2G39410.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 404.4 bits (1038), Expect = 8.6e-113
Identity = 189/285 (66.32%), Postives = 233/285 (81.75%), Query Frame = 0

Query: 51  DKEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHV 110
           ++EP+ L+F+CHGY MECSITMNS+A RL +AGFAVYG+DYEGHGKSDGL  YI++FD +
Sbjct: 32  NREPRALVFLCHGYGMECSITMNSTARRLVKAGFAVYGMDYEGHGKSDGLSAYISNFDRL 91

Query: 111 VEDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAED 170
           V+D S H+TAI ER+EN  K R++LGESMGGAV LLL RK PD+WDGA+LVAPMCKIAE+
Sbjct: 92  VDDVSTHYTAICEREENKWKMRFMLGESMGGAVVLLLGRKNPDFWDGAILVAPMCKIAEE 151

Query: 171 VKPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGN 230
           +KP P V++ILTKL  +IP WKIIP+QDII++++K PEIR QVR NP C KGRPRL T  
Sbjct: 152 MKPSPFVISILTKLISIIPKWKIIPSQDIIEISYKEPEIRKQVRENPLCSKGRPRLKTAY 211

Query: 231 ELLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGL 290
           ELLRIS DLEKRL+EVSLPF++LHG+ D+VTD +VS++L + A S DK+LK YP MWHGL
Sbjct: 212 ELLRISNDLEKRLQEVSLPFLVLHGDDDKVTDKAVSQELYKVALSADKTLKLYPGMWHGL 271

Query: 291 LYGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESDDLQTK 336
           L GET ENI++VFAD+I WLE+R   GN R E +LK  +D L  K
Sbjct: 272 LTGETPENIEIVFADVISWLEKRSDYGNDRFESELKQRNDRLNFK 316

BLAST of CmaCh13G008570 vs. TAIR 10
Match: AT2G39400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 403.3 bits (1035), Expect = 1.9e-112
Identity = 189/279 (67.74%), Postives = 228/279 (81.72%), Query Frame = 0

Query: 52  KEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHVV 111
           +EPK L+F+CHGYAME SITMNS+A RLA AGFAVYG+DYEGHGKS+GL GYI++FD +V
Sbjct: 27  QEPKALLFLCHGYAMESSITMNSAATRLANAGFAVYGMDYEGHGKSEGLNGYISNFDDLV 86

Query: 112 EDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAEDV 171
           +D SNH++ I ER+EN GK R+LLGESMGGAV LLL RKKPD+WDGAVLVAPMCK+A+++
Sbjct: 87  DDVSNHYSTICEREENKGKMRFLLGESMGGAVVLLLARKKPDFWDGAVLVAPMCKLADEI 146

Query: 172 KPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGNE 231
           KP PVV++IL KL K IPTWKI+P  DIID+A K P IR+QVR N YC+KGRPRLNT  +
Sbjct: 147 KPHPVVISILIKLAKFIPTWKIVPGNDIIDIAIKEPHIRNQVRENKYCYKGRPRLNTAYQ 206

Query: 232 LLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGLL 291
           LL +SLDLEK L +VS+PFI+LHGE D+VTD S+SK L E ASS DK+ K YP+MWH LL
Sbjct: 207 LLLVSLDLEKNLHQVSIPFIVLHGEDDKVTDKSISKMLYEVASSSDKTFKLYPKMWHALL 266

Query: 292 YGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESD 331
           YGET EN ++VF DII WLE+R    N  +E QLK + D
Sbjct: 267 YGETNENSEIVFGDIINWLEDRATDSNGGLESQLKHKHD 305

BLAST of CmaCh13G008570 vs. TAIR 10
Match: AT3G55180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 396.7 bits (1018), Expect = 1.8e-110
Identity = 182/279 (65.23%), Postives = 231/279 (82.80%), Query Frame = 0

Query: 52  KEPKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHVV 111
           +EPK LIF+CHGYAME SITM+S+A+RLA AGF+VYG+DYEGHGKS GL GY+  FD +V
Sbjct: 28  QEPKALIFLCHGYAMESSITMSSTAVRLANAGFSVYGMDYEGHGKSGGLNGYVKKFDDLV 87

Query: 112 EDCSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAEDV 171
           +D S+H+++I E +EN GK R+L+GESMGGAV LLL RKKP++WDGAVLVAPMCK+AED+
Sbjct: 88  QDVSSHYSSICELEENKGKMRFLMGESMGGAVVLLLERKKPNFWDGAVLVAPMCKLAEDI 147

Query: 172 KPKPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGNE 231
           KP P+V++ LTKL + IPTWKI+P+ DIIDVAFK   IR QVR+N YC+KGRPRL T ++
Sbjct: 148 KPHPMVISFLTKLTRFIPTWKIVPSNDIIDVAFKETHIRKQVRDNEYCYKGRPRLKTAHQ 207

Query: 232 LLRISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGLL 291
           LL +SLDLEK L++VS+PFI+LHGE D+VTD +VSK L E ASS DK+ K YP MWHGLL
Sbjct: 208 LLMVSLDLEKNLDQVSMPFIVLHGEDDKVTDKNVSKLLYEVASSSDKTFKLYPNMWHGLL 267

Query: 292 YGETVENIDVVFADIIGWLEERCGLGNSRIEKQLKTESD 331
           YGE+ EN+++VF+DII WL+ER  + N ++E +LK   D
Sbjct: 268 YGESPENLEIVFSDIISWLKERASVTNQKLETELKHVDD 306

BLAST of CmaCh13G008570 vs. TAIR 10
Match: AT3G62860.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 330.9 bits (847), Expect = 1.2e-90
Identity = 151/262 (57.63%), Postives = 199/262 (75.95%), Query Frame = 0

Query: 54  PKGLIFICHGYAMECSITMNSSAIRLARAGFAVYGIDYEGHGKSDGLQGYITSFDHVVED 113
           P+ L+F+CHGY MECS  M    IRLA AG+AV+G+DYEGHG+S G + YI  F ++V D
Sbjct: 32  PRALVFLCHGYGMECSSFMRECGIRLASAGYAVFGMDYEGHGRSKGARCYIKKFSNIVND 91

Query: 114 CSNHFTAISERKENMGKRRYLLGESMGGAVALLLHRKKPDYWDGAVLVAPMCKIAEDVKP 173
           C +++T+IS ++E   K R+L GESMGGAVALLLH+K P +W+GA+LVAPMCKI+E VKP
Sbjct: 92  CFDYYTSISAQEEYKEKGRFLYGESMGGAVALLLHKKDPSFWNGALLVAPMCKISEKVKP 151

Query: 174 KPVVVNILTKLCKVIPTWKIIPTQDIIDVAFKVPEIRHQVRNNPYCFKGRPRLNTGNELL 233
            PVV+N+LT++  +IP WKI+PT+D+ID AFK P  R ++RNN   ++ +PRL T  E+L
Sbjct: 152 HPVVINLLTRVEDIIPKWKIVPTKDVIDAAFKDPVKREEIRNNKLIYQDKPRLKTALEML 211

Query: 234 RISLDLEKRLEEVSLPFIILHGEADRVTDISVSKQLLEKASSWDKSLKTYPEMWHGLLYG 293
           R S+DLE  L E++LPF +LHGEAD VTD  +SK L EKAS+ DK++K YP MWHGL  G
Sbjct: 212 RTSMDLEDTLHEITLPFFVLHGEADIVTDPEISKALFEKASTRDKTIKLYPGMWHGLTSG 271

Query: 294 ETVENIDVVFADIIGWLEERCG 316
           E   N+D+VFADI+ WL+ R G
Sbjct: 272 EPDANVDLVFADIVNWLDARTG 293

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C9423.3e-4034.32Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1[more]
O074271.9e-2430.30Monoacylglycerol lipase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv... [more]
A0QNZ71.2e-2126.05Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(... [more]
Q996851.5e-2130.77Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2[more]
O356787.6e-2127.31Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KJY02.4e-163100.00caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111495282 PE=4... [more]
A0A6J1ECU43.0e-16198.25caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111431373 PE... [more]
A0A6J1GSV75.4e-14788.07caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111457223 PE... [more]
A0A6J1JZ462.3e-14587.37caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111490153 PE=4... [more]
A0A6J1DU623.0e-14586.67caffeoylshikimate esterase-like OS=Momordica charantia OX=3673 GN=LOC111024458 P... [more]
Match NameE-valueIdentityDescription
XP_023001020.15.0e-163100.00caffeoylshikimate esterase-like [Cucurbita maxima][more]
XP_023520138.12.7e-16198.60caffeoylshikimate esterase-like [Cucurbita pepo subsp. pepo][more]
KAG7019788.16.1e-16198.25Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022923765.16.1e-16198.25caffeoylshikimate esterase-like [Cucurbita moschata][more]
XP_038894800.11.3e-14789.47caffeoylshikimate esterase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G39420.14.4e-11769.72alpha/beta-Hydrolases superfamily protein [more]
AT2G39410.28.6e-11366.32alpha/beta-Hydrolases superfamily protein [more]
AT2G39400.11.9e-11267.74alpha/beta-Hydrolases superfamily protein [more]
AT3G55180.11.8e-11065.23alpha/beta-Hydrolases superfamily protein [more]
AT3G62860.11.2e-9057.63alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 134..147
score: 50.74
coord: 83..98
score: 36.85
coord: 250..264
score: 29.66
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 41..315
e-value: 6.6E-52
score: 178.6
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 47..311
IPR022742Serine aminopeptidase, S33PFAMPF12146Hydrolase_4coord: 53..291
e-value: 1.6E-62
score: 210.9
NoneNo IPR availablePANTHERPTHR11614:SF176ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 50..329
NoneNo IPR availablePANTHERPTHR11614PHOSPHOLIPASE-RELATEDcoord: 50..329

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh13G008570.1CmaCh13G008570.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0016298 lipase activity