CmaCh13G006570 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh13G006570
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionpolyphenol oxidase, chloroplastic-like
LocationCma_Chr13: 6171995 .. 6174261 (+)
RNA-Seq ExpressionCmaCh13G006570
SyntenyCmaCh13G006570
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGTGGGATTTGAAATAAACCATCTTTGATCCAATAACCAAGCAATGGCTTCTCTACCACCACCAGCTCTTTCCACCGCCACATTTCGCCCATCATCCAGGCCTCCAACATCAAAATACCTTCCCAACAACGCTGCTCGAGTTTTGTGTAGCGCCGCCAATGGCAAAATTGATAGAAGAGACGTTCTTATTGGGATAGGCGCAGGCGGTATCTACGGAGCTTCGGCCTTCACCAAAGATTCCACCTTTGCCCTTGCCGCTCCAGTCCAAACTCCCAACATTAGCAAGTGTGGCCCACCAGACCTGCCACCTGGCTCCGATCCAACCAACTGCTGCCCGCCGCCGTCCCCCTCTATCGTCGACTTCCGACCACCGGCGCCGGGAAAACTACGGGTCAGGCCAGCTGCCCATTTGGCGGATCAAAGCTACATAGACAAATATAAGAGAGCCGTGGAGCTAATGAAAGGGCTGCCCGGAGATGACCCACGTAGCTTCATGCAACAAGCGAACGTTCACTGTGCTTATTGTAACGGAGGGTACGACCAAGTGGGGTTTCCAGTTGAGGTGCAAGTTCATAACTCGTGGCTGTTTTTCCCATTTCACCGTTATTATCTCTACTTTTACGAGAAGATATTGGGAGAGCTGATTGGAGATCCCACTTTCGCCTTGCCCTTTTGGAACTACGATGCTCCTGGGGGTATGAGAATGCCAGCCATGTACGCCGACCACAACTCCTCGCTTTATGACCAACTTCGGAACCAAAATCATCTGCCGCCAACATTGATGGATCTGGATTTCGATGGGGTTGATTCAAACATAGGCGATAATGCGCAGATAAGAAGTAACCTAAGAATTATGTATCGCCAGATGGTTTCCAATAGTCGTACCCCAAAGCTGTTTTTTGGCAGCGCTTATGTAGCCGGAGACGAGCCATCACCAGGTAATATTTTAGTATAACATTATTTACTGCATAAATTTTAGTGATTTTAATTTTTATTTAGCCAATGTTTTTCTTTCTCCCAACTTTTTACATTATTCCATCATTTTTTTTAATTATAGATTAATTTTAACTTCAAAAGTGAAAAAAATTAATTAGTATGTTGGATAATAAAAGTCTAACTTAGGTTATAGGTTATTTTAGAGAAGGATCATTAGTCTATAAACAAATAATAAAAGTCTATAAATTTGATACTCATGTTCAAATTTGACAATATCATATCATTGTCAAAGTCATGTTGGTCTAACGGAATATACCCAAAATGATAGGAGGTGGGTCCGTGGAGAACATCCCACACAATACGGTTCACACATGGTGCGGCGACAACAACGAGCCCAACCTAGAGAACATGGGAAACTTCTACTCCGCGGCCAGAGATCCCATCTTCTACTCCCACCACTCCAACGTAGACCGCATGTGGTCCGTCTGGAAGACCCTCGGCGGAAGGCGACGGGACCTACAATACCCTGAATGGCTCGACGCCTCTTTCATTTTCTACAACGAGAAGGCCCAAGCCGTGCGAGTCCGAGTCCGAGACTGTCTCGACACCAAAAACCTCGGCTACATCTACCAAGACGTCGAACTCCCATGGCTCGAAACCCGCCCCACGCCTCGTCGAGCCCTCAGAGCCCAGAAAAGCGTCGACAGTAATGTCCCGTCCAGGCGCGTCAAACCGCCAAAGATGAAGTTACCGGTGACTCTGAAGTCGGCGATCAGTACGGAAGTGAAGAGAGGGAAGAAATCGAGAGGCAGGAAGGAGAAAGATGAGGAAGAGGAGATTTTGGTGATTAAGGGAATCGAATTCGACCCCAACACGTCCATTAAATTGGATATTTATATCAATGACGAAGATGACAGGGAAATTAGAGGCGATAATACAGAGTTTGCTGGGAGCTTTGTAAACGTGCCGCATAAACACGGGCACAAGAAGAAGGTGAAGACGGGGCTTAGATTGGGGATAAGTGAAGTGCTTGAGGATTTGGATGCTGATGATGACGACAGCATCATCGTCACTATCATCCCCAGGCTTGGAGCTGGGCTCGTCTCCATCGCTGAGATCAAGATCGAATATGGAGATTAATTTTAAATTATCATATCTCTCCCTGTTTGTTTATTACTCATTTTTATGTTCTAAGATTTCAAAGTACTTTAATGATAGTCATAAAAATAATCTAGATGCTTAATGATTGTAGATATGATATACCATATTCCTCCAGTTTTGTGCTGCGCCGGACATTATTGATACACATTTTTGGACCTTTAACTCTCTGC

mRNA sequence

AGTGGGATTTGAAATAAACCATCTTTGATCCAATAACCAAGCAATGGCTTCTCTACCACCACCAGCTCTTTCCACCGCCACATTTCGCCCATCATCCAGGCCTCCAACATCAAAATACCTTCCCAACAACGCTGCTCGAGTTTTGTGTAGCGCCGCCAATGGCAAAATTGATAGAAGAGACGTTCTTATTGGGATAGGCGCAGGCGGTATCTACGGAGCTTCGGCCTTCACCAAAGATTCCACCTTTGCCCTTGCCGCTCCAGTCCAAACTCCCAACATTAGCAAGTGTGGCCCACCAGACCTGCCACCTGGCTCCGATCCAACCAACTGCTGCCCGCCGCCGTCCCCCTCTATCGTCGACTTCCGACCACCGGCGCCGGGAAAACTACGGGTCAGGCCAGCTGCCCATTTGGCGGATCAAAGCTACATAGACAAATATAAGAGAGCCGTGGAGCTAATGAAAGGGCTGCCCGGAGATGACCCACGTAGCTTCATGCAACAAGCGAACGTTCACTGTGCTTATTGTAACGGAGGGTACGACCAAGTGGGGTTTCCAGTTGAGGTGCAAGTTCATAACTCGTGGCTGTTTTTCCCATTTCACCGTTATTATCTCTACTTTTACGAGAAGATATTGGGAGAGCTGATTGGAGATCCCACTTTCGCCTTGCCCTTTTGGAACTACGATGCTCCTGGGGGTATGAGAATGCCAGCCATGTACGCCGACCACAACTCCTCGCTTTATGACCAACTTCGGAACCAAAATCATCTGCCGCCAACATTGATGGATCTGGATTTCGATGGGGTTGATTCAAACATAGGCGATAATGCGCAGATAAGAAGTAACCTAAGAATTATGTATCGCCAGATGGTTTCCAATAGTCGTACCCCAAAGCTGTTTTTTGGCAGCGCTTATGTAGCCGGAGACGAGCCATCACCAGGAGGTGGGTCCGTGGAGAACATCCCACACAATACGGTTCACACATGGTGCGGCGACAACAACGAGCCCAACCTAGAGAACATGGGAAACTTCTACTCCGCGGCCAGAGATCCCATCTTCTACTCCCACCACTCCAACGTAGACCGCATGTGGTCCGTCTGGAAGACCCTCGGCGGAAGGCGACGGGACCTACAATACCCTGAATGGCTCGACGCCTCTTTCATTTTCTACAACGAGAAGGCCCAAGCCGTGCGAGTCCGAGTCCGAGACTGTCTCGACACCAAAAACCTCGGCTACATCTACCAAGACGTCGAACTCCCATGGCTCGAAACCCGCCCCACGCCTCGTCGAGCCCTCAGAGCCCAGAAAAGCGTCGACAGTAATGTCCCGTCCAGGCGCGTCAAACCGCCAAAGATGAAGTTACCGGTGACTCTGAAGTCGGCGATCAGTACGGAAGTGAAGAGAGGGAAGAAATCGAGAGGCAGGAAGGAGAAAGATGAGGAAGAGGAGATTTTGGTGATTAAGGGAATCGAATTCGACCCCAACACGTCCATTAAATTGGATATTTATATCAATGACGAAGATGACAGGGAAATTAGAGGCGATAATACAGAGTTTGCTGGGAGCTTTGTAAACGTGCCGCATAAACACGGGCACAAGAAGAAGGTGAAGACGGGGCTTAGATTGGGGATAAGTGAAGTGCTTGAGGATTTGGATGCTGATGATGACGACAGCATCATCGTCACTATCATCCCCAGGCTTGGAGCTGGGCTCGTCTCCATCGCTGAGATCAAGATCGAATATGGAGATTAATTTTAAATTATCATATCTCTCCCTGTTTGTTTATTACTCATTTTTATGTTCTAAGATTTCAAAGTACTTTAATGATAGTCATAAAAATAATCTAGATGCTTAATGATTGTAGATATGATATACCATATTCCTCCAGTTTTGTGCTGCGCCGGACATTATTGATACACATTTTTGGACCTTTAACTCTCTGC

Coding sequence (CDS)

ATGGCTTCTCTACCACCACCAGCTCTTTCCACCGCCACATTTCGCCCATCATCCAGGCCTCCAACATCAAAATACCTTCCCAACAACGCTGCTCGAGTTTTGTGTAGCGCCGCCAATGGCAAAATTGATAGAAGAGACGTTCTTATTGGGATAGGCGCAGGCGGTATCTACGGAGCTTCGGCCTTCACCAAAGATTCCACCTTTGCCCTTGCCGCTCCAGTCCAAACTCCCAACATTAGCAAGTGTGGCCCACCAGACCTGCCACCTGGCTCCGATCCAACCAACTGCTGCCCGCCGCCGTCCCCCTCTATCGTCGACTTCCGACCACCGGCGCCGGGAAAACTACGGGTCAGGCCAGCTGCCCATTTGGCGGATCAAAGCTACATAGACAAATATAAGAGAGCCGTGGAGCTAATGAAAGGGCTGCCCGGAGATGACCCACGTAGCTTCATGCAACAAGCGAACGTTCACTGTGCTTATTGTAACGGAGGGTACGACCAAGTGGGGTTTCCAGTTGAGGTGCAAGTTCATAACTCGTGGCTGTTTTTCCCATTTCACCGTTATTATCTCTACTTTTACGAGAAGATATTGGGAGAGCTGATTGGAGATCCCACTTTCGCCTTGCCCTTTTGGAACTACGATGCTCCTGGGGGTATGAGAATGCCAGCCATGTACGCCGACCACAACTCCTCGCTTTATGACCAACTTCGGAACCAAAATCATCTGCCGCCAACATTGATGGATCTGGATTTCGATGGGGTTGATTCAAACATAGGCGATAATGCGCAGATAAGAAGTAACCTAAGAATTATGTATCGCCAGATGGTTTCCAATAGTCGTACCCCAAAGCTGTTTTTTGGCAGCGCTTATGTAGCCGGAGACGAGCCATCACCAGGAGGTGGGTCCGTGGAGAACATCCCACACAATACGGTTCACACATGGTGCGGCGACAACAACGAGCCCAACCTAGAGAACATGGGAAACTTCTACTCCGCGGCCAGAGATCCCATCTTCTACTCCCACCACTCCAACGTAGACCGCATGTGGTCCGTCTGGAAGACCCTCGGCGGAAGGCGACGGGACCTACAATACCCTGAATGGCTCGACGCCTCTTTCATTTTCTACAACGAGAAGGCCCAAGCCGTGCGAGTCCGAGTCCGAGACTGTCTCGACACCAAAAACCTCGGCTACATCTACCAAGACGTCGAACTCCCATGGCTCGAAACCCGCCCCACGCCTCGTCGAGCCCTCAGAGCCCAGAAAAGCGTCGACAGTAATGTCCCGTCCAGGCGCGTCAAACCGCCAAAGATGAAGTTACCGGTGACTCTGAAGTCGGCGATCAGTACGGAAGTGAAGAGAGGGAAGAAATCGAGAGGCAGGAAGGAGAAAGATGAGGAAGAGGAGATTTTGGTGATTAAGGGAATCGAATTCGACCCCAACACGTCCATTAAATTGGATATTTATATCAATGACGAAGATGACAGGGAAATTAGAGGCGATAATACAGAGTTTGCTGGGAGCTTTGTAAACGTGCCGCATAAACACGGGCACAAGAAGAAGGTGAAGACGGGGCTTAGATTGGGGATAAGTGAAGTGCTTGAGGATTTGGATGCTGATGATGACGACAGCATCATCGTCACTATCATCCCCAGGCTTGGAGCTGGGCTCGTCTCCATCGCTGAGATCAAGATCGAATATGGAGATTAA

Protein sequence

MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAANGKIDRRDVLIGIGAGGIYGASAFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGKLRVRPAAHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQNHLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRRDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQKSVDSNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPNTSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLDADDDDSIIVTIIPRLGAGLVSIAEIKIEYGD
Homology
BLAST of CmaCh13G006570 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 9.6e-202
Identity = 346/596 (58.05%), Postives = 435/596 (72.99%), Query Frame = 0

Query: 1   MASLPPPALSTATFRPSSRPPTSKYLPNN-------------AARVLCSAAN-------- 60
           M SL PP ++T T    +  P S +  NN             A +V C A N        
Sbjct: 1   MTSLSPPVVTTPTVPNPATKPLSPFSQNNSQVSLLTKPKRSFARKVSCKATNNDQNDQAQ 60

Query: 61  GKIDRRDVLIGIGAGGIYGASAFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPP 120
            K+DRR+VL+G+  GG+YG +    D  FA A P+  P++SKCGP DLP G+ PTNCCPP
Sbjct: 61  SKLDRRNVLLGL--GGLYGVAGMGTD-PFAFAKPIAPPDVSKCGPADLPQGAVPTNCCPP 120

Query: 121 PSPSIVDFRPPAPGKLRVRPAAHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCA 180
           PS  I+DF+ PAP KLR+RP AH  DQ+Y DKY +A+ELMK LP DDPRSF QQA VHCA
Sbjct: 121 PSTKIIDFKLPAPAKLRIRPPAHAVDQAYRDKYYKAMELMKALPDDDPRSFKQQAAVHCA 180

Query: 181 YCNGGYDQVGFP-VEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGG 240
           YC+G YDQVGFP +E+Q+HNSWLFFPFHRYYLYF+EKILG+LI DPTFALPFWN+D+P G
Sbjct: 181 YCDGAYDQVGFPELELQIHNSWLFFPFHRYYLYFFEKILGKLINDPTFALPFWNWDSPAG 240

Query: 241 MRMPAMYADHNSSLYDQLRNQNHLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSN 300
           M +PA+YAD  S LYD+LR+ NH PPTL+DLD++G + N+     I +NL+IMYRQMVSN
Sbjct: 241 MPLPAIYADPKSPLYDKLRSANHQPPTLVDLDYNGTEDNVSKETTINANLKIMYRQMVSN 300

Query: 301 SRTPKLFFGSAYVAGDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIF 360
           S+  KLFFG+ Y AGDEP PGGGS+E  PH  VH W GDN +PN E+MGNFYSA RDPIF
Sbjct: 301 SKNAKLFFGNPYRAGDEPDPGGGSIEGTPHAPVHLWTGDNTQPNFEDMGNFYSAGRDPIF 360

Query: 361 YSHHSNVDRMWSVWKTLGGRRRDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYI 420
           ++HHSNVDRMWS+WKTLGG+R DL   +WLD+ F+FYNE A+ VRV+VRDCL+TKNLGY+
Sbjct: 361 FAHHSNVDRMWSIWKTLGGKRTDLTDSDWLDSGFLFYNENAELVRVKVRDCLETKNLGYV 420

Query: 421 YQDVELPWLETRPTPRRALRAQKSV-------DSNVPSRRVKPPKMKLPVTLKSAISTEV 480
           YQDV++PWL ++PTPRRA  A   V        + V S        + P++L S IST V
Sbjct: 421 YQDVDIPWLSSKPTPRRAKVALSKVAKKLGVAHAAVASSSKVVAGTEFPISLGSKISTVV 480

Query: 481 KRGK-KSRGRKEKDEEEEILVIKGIEFDPNTSIKLDIYINDEDDREIRGDNTEFAGSFVN 540
           KR K K R +K K++EEEILVI+GIEFD + ++K D+Y+ND DD     D TEFAGSFV+
Sbjct: 481 KRPKQKKRSKKAKEDEEEILVIEGIEFDRDVAVKFDVYVNDVDDLPSGPDKTEFAGSFVS 540

Query: 541 VPHKHGHKKKVKTGLRLGISEVLEDLDADDDDSIIVTIIPRLGAGLVSIAEIKIEY 567
           VPH H HKKK+ T LRLG++++LE+++A+DDDS++VT++P+ GA  V I  IKIE+
Sbjct: 541 VPHSHKHKKKMNTILRLGLTDLLEEIEAEDDDSVVVTLVPKFGA--VKIGGIKIEF 591

BLAST of CmaCh13G006570 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 1.1e-173
Identity = 306/544 (56.25%), Postives = 393/544 (72.24%), Query Frame = 0

Query: 44  RRDVLIGIGAGGIYGASAFTKDSTFALAAPVQTPNISKCGPP-DLPPGSDP--TNCCPPP 103
           RR+VLIG+  GGIYG  A    +  ALA+P+  P++SKC PP DLP G+ P   NCCPP 
Sbjct: 70  RRNVLIGL--GGIYGTLA---TNPSALASPISPPDLSKCVPPSDLPSGTTPPNINCCPPY 129

Query: 104 SPSIVDFRPPAPGKLRVRPAAHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAY 163
           S  I DF+ P+   LRVR AAHL D  +++KYK+A ELMK LP +DPR+F QQAN+HCAY
Sbjct: 130 STKITDFKFPSNQPLRVRQAAHLVDNEFLEKYKKATELMKALPSNDPRNFTQQANIHCAY 189

Query: 164 CNGGYDQVGFP-VEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGM 223
           C+G Y Q+GFP +++QVH SWLFFPFHR+YLYFYE+ILG LI DPTFALPFWNYDAP GM
Sbjct: 190 CDGAYSQIGFPDLKLQVHGSWLFFPFHRWYLYFYERILGSLINDPTFALPFWNYDAPDGM 249

Query: 224 RMPAMYADHNSSLYDQLRNQNHLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNS 283
           ++P +YAD  S LYD+LRN +H PPTL+DL+F  + S+I  N  I++NL IMYRQ+ SN 
Sbjct: 250 QLPTIYADKASPLYDELRNASHQPPTLIDLNFCDIGSDIDRNELIKTNLSIMYRQVYSNG 309

Query: 284 RTPKLFFGSAYVAGDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFY 343
           +T +LF G+ Y AGD    G GS+EN+PH  VHTW GDN + N+E+MG FYSAARDPIFY
Sbjct: 310 KTSRLFLGNPYRAGDAEPQGAGSIENVPHAPVHTWTGDNTQTNIEDMGIFYSAARDPIFY 369

Query: 344 SHHSNVDRMWSVWKTLGGRRRDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIY 403
           SHHSNVDR+W +WKTLGG++ D    +WL++ F+FY+E    VRV V+D LD   LGY Y
Sbjct: 370 SHHSNVDRLWYIWKTLGGKKHDFTDKDWLESGFLFYDENKNLVRVNVKDSLDIDKLGYAY 429

Query: 404 QDVELPWLETRPTPRRALRAQKSVDSNVPS------------RRVKPPK-MKLPVTLKSA 463
           QDV +PW + +P PRR  + QK V+  V              R+  P K +  P+ L + 
Sbjct: 430 QDVPIPWEKAKPVPRRT-KVQKLVEVEVNDGNLRKSPTIFLVRQQSPRKYVTFPLVLNNK 489

Query: 464 ISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPNTSIKLDIYINDEDDREIRGDNTEFAG 523
           +S  VKR KK R +KEK+EEEE+LVI+GIEF  N +IK D+YINDEDD+ +   NTEFAG
Sbjct: 490 VSAIVKRPKKLRSKKEKEEEEEVLVIEGIEFYMNIAIKFDVYINDEDDK-VGAGNTEFAG 549

Query: 524 SFVNVPHK-HGHK-KKVKTGLRLGISEVLEDLDADDDDSIIVTIIPRLGAGLVSIAEIKI 569
           SFVN+PH  HGHK KK+ T LRLGI+++LEDL  + DD+I+VT++P+ G+G V I  ++I
Sbjct: 550 SFVNIPHSAHGHKNKKIITSLRLGITDLLEDLHVEGDDNIVVTLVPKCGSGQVKINNVEI 606

BLAST of CmaCh13G006570 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 7.0e-168
Identity = 306/588 (52.04%), Postives = 397/588 (67.52%), Query Frame = 0

Query: 5   PPPALSTATFRPSSRPPTSKYLPNNAARVLCSAAN----------------GKIDRRDVL 64
           P P    A+  P +R  + ++ P   ++V C++AN                GK+DRR+VL
Sbjct: 25  PSPLFQRASHVPVARNRSRRFAP---SKVSCNSANGDPNSDSTSDVRETSSGKLDRRNVL 84

Query: 65  IGIGAGGIYGAS-AFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDF 124
           +GI  GG+YGA+         A  AP+Q P+ISKCG   +P G  PTNCCPP +  I+DF
Sbjct: 85  LGI--GGLYGAAGGLGATKPLAFGAPIQAPDISKCGTATVPDGVTPTNCCPPVTTKIIDF 144

Query: 125 RPPAPGK-LRVRPAAHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYD 184
           + P+ G  +R RPAAHL  + Y+ KYK+A+EL K LP DDPRSF QQANVHC YC G YD
Sbjct: 145 QLPSSGSPMRTRPAAHLVSKEYLAKYKKAIELQKALPDDDPRSFKQQANVHCTYCQGAYD 204

Query: 185 QVGF-PVEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMY 244
           QVG+  +E+QVH SWLF PFHRYYLYF E+IL +LI DPTFALP+W +D P GM MP +Y
Sbjct: 205 QVGYTDLELQVHASWLFLPFHRYYLYFNERILAKLIDDPTFALPYWAWDNPDGMYMPTIY 264

Query: 245 ADHNSSLYDQLRNQNHLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLF 304
           A   SSLYD+ RN  HLPPT++DLD+DG +  I D+     NL IMY+Q+VS + TPKLF
Sbjct: 265 ASSPSSLYDEKRNAKHLPPTVIDLDYDGTEPTIPDDELKTDNLAIMYKQIVSGATTPKLF 324

Query: 305 FGSAYVAGDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNV 364
            G  Y AGD   PG G++E+ PHN VH W G  ++P+ E+MGNFY+A RDPIF+ HH+NV
Sbjct: 325 LGYPYRAGDAIDPGAGTLEHAPHNIVHKWTGLADKPS-EDMGNFYTAGRDPIFFGHHANV 384

Query: 365 DRMWSVWKTLGGR-RRDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVEL 424
           DRMW++WKT+GG+ R+D    +WLDA+F+FY+E  Q V+V+V DC+DT  L Y YQD+ +
Sbjct: 385 DRMWNIWKTIGGKNRKDFTDTDWLDATFVFYDENKQLVKVKVSDCVDTSKLRYQYQDIPI 444

Query: 425 PWLETRPTPRRALRAQKSVDSNVPSRRVKPPKMKL------PVTLKSAISTEVKRGKKSR 484
           PWL  + T  +A    KS  S V ++  + PK  +      P  L S I  EV R KKSR
Sbjct: 445 PWL-PKNTKAKAKTTTKSSKSGV-AKAAELPKTTISSIGDFPKALNSVIRVEVPRPKKSR 504

Query: 485 GRKEKDEEEEILVIKGIEFDPNTSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHK 544
            +KEK++EEE+L+IKGIE D    +K D+YINDED    R  N+EFAGSFVNVPHKH  +
Sbjct: 505 SKKEKEDEEEVLLIKGIELDRENFVKFDVYINDEDYSVSRPKNSEFAGSFVNVPHKHMKE 564

Query: 545 KKVKTGLRLGISEVLEDLDADDDDSIIVTIIPRLGAGLVSIAEIKIEY 567
            K KT LR  I+E+LEDL A+DD+S+IVTI+PR G   V+I  I+IE+
Sbjct: 565 MKTKTNLRFAINELLEDLGAEDDESVIVTIVPRAGGDDVTIGGIEIEF 604

BLAST of CmaCh13G006570 vs. ExPASy Swiss-Prot
Match: Q9FRX6 (Aureusidin synthase OS=Antirrhinum majus OX=4151 GN=AS1 PE=1 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 2.2e-161
Identity = 289/528 (54.73%), Postives = 368/528 (69.70%), Query Frame = 0

Query: 47  VLIGIGAGGIYGASAFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVD 106
           +++G+        + F   ST     P+  P+++KCG PDLPPG+ P NCCPP    I+D
Sbjct: 40  LIVGLYIANSLAYARFASTST----GPIAAPDVTKCGQPDLPPGTAPINCCPPIPAKIID 99

Query: 107 FR-PPAPGKLRVRPAAHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGY 166
           F  PP    +RVR AAHL D +YI K+K+AVELM+ LP DDPRSF QQANVHCAYC G Y
Sbjct: 100 FELPPPSTTMRVRRAAHLVDDAYIAKFKKAVELMRALPEDDPRSFKQQANVHCAYCAGAY 159

Query: 167 DQVGFP-VEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAM 226
           +Q GF  +++Q+H SWLFFPFHRYY+YF+E+ILG+LI D TFALPFWNYD+PGGM +P+M
Sbjct: 160 NQAGFTNLKLQIHRSWLFFPFHRYYIYFFERILGKLINDTTFALPFWNYDSPGGMTIPSM 219

Query: 227 YADHNSSLYDQLRNQNHLPPTLMDLD--FDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTP 286
           + D NSSLYD LR+ NH PPT++DL+  F   D+      Q+  NL+I+YRQMVS+++TP
Sbjct: 220 FIDTNSSLYDSLRDSNHQPPTIVDLNYAFSDSDNTTTPEEQMIINLKIVYRQMVSSAKTP 279

Query: 287 KLFFGSAYVAGDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHH 346
           +LFFG  Y  GD+  PG GS+E +PH  +H W G  N P  ENMG FYS ARDPIF++HH
Sbjct: 280 QLFFGRPYRRGDQEFPGVGSIELVPHGMIHLWTGSENTPYGENMGAFYSTARDPIFFAHH 339

Query: 347 SNVDRMWSVWKTLGG-RRRDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQD 406
           SNVDRMWS+WKTLGG RR DL  P++LDASF+FY+E A+ VRV+VRDCLD K LGY+YQD
Sbjct: 340 SNVDRMWSIWKTLGGPRRTDLTDPDFLDASFVFYDENAEMVRVKVRDCLDEKKLGYVYQD 399

Query: 407 VELPWLETRPTPRRALRAQKSVDSNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRK 466
           VE+PWL TRPTP    +   S+           P+   P  L   +   V R KK+R RK
Sbjct: 400 VEIPWLNTRPTP----KVSPSLLKKFHRTNTANPRQVFPAILDRVLKVIVTRPKKTRSRK 459

Query: 467 EKDEEEEILVIKGIEFD-PNTSIKLDIYIN-DEDD-REIRGDNTEFAGSFVNVPHKHGHK 526
           EKDE EEILVI+GIE +  +  +K D+YIN DEDD   I  +N EFAGSFV++ HK    
Sbjct: 460 EKDELEEILVIEGIELERDHGHVKFDVYINADEDDLAVISPENAEFAGSFVSLWHKPIKG 519

Query: 527 KKVKTG-LRLGISEVLEDLDADDDDSIIVTIIPRLGAGLVSIAEIKIE 566
           K+ KT  L L I ++LEDLDAD+DD ++VT++PR     + I  +KIE
Sbjct: 520 KRTKTQLLTLSICDILEDLDADEDDYVLVTLVPRNAGDAIKIHNVKIE 559

BLAST of CmaCh13G006570 vs. ExPASy Swiss-Prot
Match: Q9ZP19 (Polyphenol oxidase I, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-1 PE=1 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 4.5e-159
Identity = 274/503 (54.47%), Postives = 354/503 (70.38%), Query Frame = 0

Query: 72  APVQTPNISKC--GPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGKLRVRPAAHLADQSYI 131
           AP+Q P ISKC   P DLPPG+   NCCPP + +IVD++ PA   ++VRPAAH  D+  I
Sbjct: 1   APIQAPEISKCVVPPADLPPGAVVDNCCPPVASNIVDYKLPAVTTMKVRPAAHTMDKDAI 60

Query: 132 DKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFP-VEVQVHNSWLFFPFHRY 191
            K+ +AVELMK LP DDPR+F QQA VHCAYCNGGYDQV FP  E+QVHNSWLFFPFHR+
Sbjct: 61  AKFAKAVELMKALPADDPRNFYQQALVHCAYCNGGYDQVNFPDQEIQVHNSWLFFPFHRW 120

Query: 192 YLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQNHLPPTLMD 251
           YLYFYE+ILG+LIGDP+F LPFWN+D PGGM +P    D  SSLYD  RNQ+HLPP ++D
Sbjct: 121 YLYFYERILGKLIGDPSFGLPFWNWDNPGGMVLPDFLNDSTSSLYDSNRNQSHLPPVVVD 180

Query: 252 LDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGGGSVENIPH 311
           L ++G D+++ D  +I  NL +MY+QMV+N+ T +LF G AY AGD PSPG GS+E  PH
Sbjct: 181 LGYNGADTDVTDQQRITDNLALMYKQMVTNAGTAELFLGKAYRAGDAPSPGAGSIETSPH 240

Query: 312 NTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGG--RRRDLQYPE 371
             +H W GD    N E+MGNFYSA RD  FY HHSNVDRMW++W+ L G  R+RD    +
Sbjct: 241 IPIHRWVGDPRNTNNEDMGNFYSAGRDIAFYCHHSNVDRMWTIWQQLAGKPRKRDYTDSD 300

Query: 372 WLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTP--RRALRAQKSVD 431
           WL+A+F+FY+E  QAV+VR+ D LD + +GY Y    LPWL+++P P  ++   A KS  
Sbjct: 301 WLNATFLFYDENGQAVKVRIGDSLDNQKMGYKYAKTPLPWLDSKPVPTKKKGGYASKSKA 360

Query: 432 SNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPNTSIK 491
             V S          PVTL   +  +V R KKSR  +EK+ EEEIL+I GIE + +   K
Sbjct: 361 PFVAS--------VFPVTLDKVVQVKVARPKKSRSAEEKEAEEEILLIVGIEVEIDKYAK 420

Query: 492 LDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLDADDDDSI 551
            D+Y+ND DD     D  E+AGSF ++PHKH   KK++T L LG++E LEDL A+DDD+I
Sbjct: 421 FDVYLNDSDDPSGGKDKAEYAGSFAHLPHKHKGMKKIRTTLSLGLNEPLEDLGAEDDDTI 480

Query: 552 IVTIIPRLGAGLVSIAEIKIEYG 568
           +VT+ P++G G+VS+  +K+ YG
Sbjct: 481 LVTLAPKVGGGVVSVDNVKVVYG 495

BLAST of CmaCh13G006570 vs. ExPASy TrEMBL
Match: A0A6J1KRC0 (polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495765 PE=3 SV=1)

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0

Query: 1   MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAANGKIDRRDVLIGIGAGGIYGAS 60
           MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAANGKIDRRDVLIGIGAGGIYGAS
Sbjct: 1   MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAANGKIDRRDVLIGIGAGGIYGAS 60

Query: 61  AFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120
           AFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGKLRVRPA
Sbjct: 61  AFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120

Query: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240

Query: 241 HLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300
           HLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360
           GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360

Query: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ 420
           DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ
Sbjct: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ 420

Query: 421 KSVDSNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPN 480
           KSVDSNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPN
Sbjct: 421 KSVDSNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPN 480

Query: 481 TSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLDADD 540
           TSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLDADD
Sbjct: 481 TSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLDADD 540

Query: 541 DDSIIVTIIPRLGAGLVSIAEIKIEYGD 569
           DDSIIVTIIPRLGAGLVSIAEIKIEYGD
Sbjct: 541 DDSIIVTIIPRLGAGLVSIAEIKIEYGD 568

BLAST of CmaCh13G006570 vs. ExPASy TrEMBL
Match: A0A6J1EGP5 (polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111434015 PE=3 SV=1)

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 530/568 (93.31%), Postives = 551/568 (97.01%), Query Frame = 0

Query: 1   MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAANGKIDRRDVLIGIGAGGIYGAS 60
           MASLPPPALSTA FRPSS   TSKYLP NAAR+LCSAA+GKIDRRDVLIGIGAGG+YGAS
Sbjct: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 60

Query: 61  AFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120
           +FTKDSTFALAAPVQTPNISKCGPPDLPPG+DPTNCCPPP+ SI+DF PPAPGKLRVRPA
Sbjct: 61  SFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA 120

Query: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHLADQSYIDKYKRAVELMKGLP DDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGM+MPAMYAD NSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 240

Query: 241 HLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300
           HLPPT +DLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360
           GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSN+DRMWSVWKTLGGRR+
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 360

Query: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ 420
           DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVEL WLETRPTPRRALRAQ
Sbjct: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPRRALRAQ 420

Query: 421 KSVDSNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPN 480
           KSVDSNVPSRRVKP ++K PVT+KSA+STEVKR KKSR +KEK+EEEEILVIKGIEFDPN
Sbjct: 421 KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN 480

Query: 481 TSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLDADD 540
           T+IKLD+YINDEDD EIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDL+ADD
Sbjct: 481 TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD 540

Query: 541 DDSIIVTIIPRLGAGLVSIAEIKIEYGD 569
           DDSIIVTIIPRLGAGLVSIAEI +EYGD
Sbjct: 541 DDSIIVTIIPRLGAGLVSIAEITMEYGD 568

BLAST of CmaCh13G006570 vs. ExPASy TrEMBL
Match: A0A6J1KJD3 (polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495764 PE=3 SV=1)

HSP 1 Score: 1087.8 bits (2812), Expect = 0.0e+00
Identity = 527/568 (92.78%), Postives = 546/568 (96.13%), Query Frame = 0

Query: 1   MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAANGKIDRRDVLIGIGAGGIYGAS 60
           M SL  PALST TFRPSSRPPTSKYLPNNAARVLCSAA+GKIDRRDVLIGIGAGGIYGAS
Sbjct: 1   MGSLSSPALSTTTFRPSSRPPTSKYLPNNAARVLCSAADGKIDRRDVLIGIGAGGIYGAS 60

Query: 61  AFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120
           AFTKDSTFALAAPVQTPNISKC PPDLP G+DPTNCCPPPSPSIVDFRPP PGKLRVRPA
Sbjct: 61  AFTKDSTFALAAPVQTPNISKCSPPDLPHGADPTNCCPPPSPSIVDFRPPVPGKLRVRPA 120

Query: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQAN+HCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANIHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240

Query: 241 HLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300
           HLPPTLMDLDFDGVD  IGD+A I+SNLRIMYRQMVSNS TPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTLMDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360
           GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360

Query: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ 420
           DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTP RALRAQ
Sbjct: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPCRALRAQ 420

Query: 421 KSVDSNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPN 480
           KSVDSNVPSRRVKPPKMK PVTLKSAISTEVKRGKKSR +KEK+EEEEILVI+GI+ DPN
Sbjct: 421 KSVDSNVPSRRVKPPKMKFPVTLKSAISTEVKRGKKSRSKKEKEEEEEILVIEGIKLDPN 480

Query: 481 TSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLDADD 540
             +K DIYINDED++E R D +EFAGSFVNVPHKHGHKKK+KTGLRLGI+++LEDLDAD 
Sbjct: 481 MLVKFDIYINDEDEKENRADISEFAGSFVNVPHKHGHKKKMKTGLRLGITQLLEDLDADC 540

Query: 541 DDSIIVTIIPRLGAGLVSIAEIKIEYGD 569
           DDSIIVT+IPRLGAGLVSIA+IKIEYGD
Sbjct: 541 DDSIIVTLIPRLGAGLVSIAKIKIEYGD 568

BLAST of CmaCh13G006570 vs. ExPASy TrEMBL
Match: A0A6J1EMV7 (polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111434014 PE=3 SV=1)

HSP 1 Score: 996.1 bits (2574), Expect = 5.9e-287
Identity = 488/568 (85.92%), Postives = 514/568 (90.49%), Query Frame = 0

Query: 1   MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAANGKIDRRDVLIGIGAGGIYGAS 60
           MASL PPALST TFRPSS   TSKYLPNNAAR+LCSAA+G IDRRDVLIGIGAGG+YGAS
Sbjct: 1   MASLSPPALSTTTFRPSSSLRTSKYLPNNAARILCSAADGNIDRRDVLIGIGAGGLYGAS 60

Query: 61  AFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120
           A      FAL APVQTPNISKCGPPDLPP +DPTNCCPPPSPSIVDFRPPAPGKLRVRPA
Sbjct: 61  A------FALGAPVQTPNISKCGPPDLPPDADPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120

Query: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHL DQSYIDKYKRAVELMKGLP DDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLVDQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPT ALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTLALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240

Query: 241 HLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300
           HLPPTL+DLDFDGVD  IGD+A I+SNLRIMYRQMVSNS TPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTLIDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360
           GSVENIPHNTVHTWCGDN +PNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRR+
Sbjct: 301 GSVENIPHNTVHTWCGDNKKPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRQ 360

Query: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ 420
           DLQ PEWL+ASFIFYNEKAQAVRVRVRDCLDTK LGY+YQDVELPWL+TRP PR A +A 
Sbjct: 361 DLQNPEWLNASFIFYNEKAQAVRVRVRDCLDTKKLGYVYQDVELPWLQTRPMPRGARKA- 420

Query: 421 KSVDSNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPN 480
                       + PKMK PVTLKSAISTEVKR KKSR +KEK+EEEEILVI+GI+ DPN
Sbjct: 421 -----------ARHPKMKFPVTLKSAISTEVKRAKKSRSKKEKEEEEEILVIEGIKLDPN 480

Query: 481 TSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLDADD 540
             +K DIYINDED++E R D TEFAGSFVNVPHKHGH KKVKTGLRLGI+E+LEDL AD 
Sbjct: 481 MLVKFDIYINDEDEKENRADITEFAGSFVNVPHKHGHNKKVKTGLRLGITELLEDLGADG 540

Query: 541 DDSIIVTIIPRLGAGLVSIAEIKIEYGD 569
           DDSIIVT++PRLGAGLVSIA+IKIEYGD
Sbjct: 541 DDSIIVTLVPRLGAGLVSIAKIKIEYGD 550

BLAST of CmaCh13G006570 vs. ExPASy TrEMBL
Match: A0A1L5JHU3 (Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.9e-257
Identity = 440/594 (74.07%), Postives = 500/594 (84.18%), Query Frame = 0

Query: 1   MASLPPPALSTAT------FRPSSRPP-----TSKYLPNNAARVLCSAANG---KIDRRD 60
           MA+ PP   S AT      F P+++ P      +KY  + A R+LCSA+NG    IDRRD
Sbjct: 1   MATSPPALSSAATTTTFGPFLPNNKNPPPNLRRTKYFSSKAPRILCSASNGGQNHIDRRD 60

Query: 61  VLIGIGAGGIYGASAFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVD 120
           +LIG+  GG+YGAS  ++ S  A AAPVQTPNISKCG PDLPPG+DP NCCPPP+ ++VD
Sbjct: 61  ILIGL--GGLYGASTLSQGS-LAFAAPVQTPNISKCGQPDLPPGADPINCCPPPTTAVVD 120

Query: 121 FRPPAPGKLRVRPAAHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYD 180
           FRP APGKLRVRPAAHLADQ+Y++KYKRAVELMK LP DDPRSF QQANVHCAYCNG YD
Sbjct: 121 FRPQAPGKLRVRPAAHLADQNYVEKYKRAVELMKALPDDDPRSFKQQANVHCAYCNGAYD 180

Query: 181 QVGFPVEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYA 240
           QVGFPVEVQVHNSWLFFPFHRYYL+F+EKILGELIGDP+FALPFWNYDAPGGM+MPAMYA
Sbjct: 181 QVGFPVEVQVHNSWLFFPFHRYYLHFHEKILGELIGDPSFALPFWNYDAPGGMKMPAMYA 240

Query: 241 DHNSSLYDQLRNQNHLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFF 300
           D  SSLYD+LRN+NHLPPTL+DLDF GVD  IGD AQIRSNL IMYRQMVSNSRTP LFF
Sbjct: 241 DEKSSLYDELRNRNHLPPTLVDLDFGGVDPTIGDEAQIRSNLSIMYRQMVSNSRTPSLFF 300

Query: 301 GSAYVAGDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVD 360
           G+AY+AGDEPSPGGG VENIPH  VH WCGDNNEPN ENMGNFYSAARDPIFY+HHSN+D
Sbjct: 301 GNAYLAGDEPSPGGGLVENIPHGPVHIWCGDNNEPNFENMGNFYSAARDPIFYAHHSNID 360

Query: 361 RMWSVWKTLGGRRRDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPW 420
           R+WSVWKTLGGRR+DLQ  +WL+ASFIFY+EKAQAVRVRVRDCLD++NLGY+YQDV+LPW
Sbjct: 361 RLWSVWKTLGGRRQDLQNADWLNASFIFYDEKAQAVRVRVRDCLDSRNLGYVYQDVQLPW 420

Query: 421 LETRPTPRRALRAQKSV--------------DSNVPSRRVKPPKMKLPVTLKSAISTEVK 480
           LE+RPTPRRALRAQK+V              +    S R++P K+K P+TL S IS EVK
Sbjct: 421 LESRPTPRRALRAQKAVAHLPFGVGPALAAGEPGPSSSRLRPAKIKFPITLNSPISMEVK 480

Query: 481 RGKKSRGRKEKDEEEEILVIKGIEFDPNTSIKLDIYINDEDDREIRGDNTEFAGSFVNVP 540
           R K+SR +KEKDEEEEI+VI GIEFD N +IK D++INDEDD+EIR DNTEFAGSFVNVP
Sbjct: 481 RPKRSRSKKEKDEEEEIIVIYGIEFDANKAIKFDVFINDEDDKEIRPDNTEFAGSFVNVP 540

Query: 541 HKHGHKKKVKTGLRLGISEVLEDLDADDDDSIIVTIIPRLGAGLVSIAEIKIEY 567
           HKHGHKK VKTGLRLGI+E+LEDLDADDDDS+ VT++PR G GLVSIA IKIE+
Sbjct: 541 HKHGHKKNVKTGLRLGITELLEDLDADDDDSVRVTLVPRSGKGLVSIAGIKIEF 591

BLAST of CmaCh13G006570 vs. NCBI nr
Match: XP_023001713.1 (polyphenol oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0

Query: 1   MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAANGKIDRRDVLIGIGAGGIYGAS 60
           MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAANGKIDRRDVLIGIGAGGIYGAS
Sbjct: 1   MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAANGKIDRRDVLIGIGAGGIYGAS 60

Query: 61  AFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120
           AFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGKLRVRPA
Sbjct: 61  AFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120

Query: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240

Query: 241 HLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300
           HLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360
           GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360

Query: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ 420
           DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ
Sbjct: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ 420

Query: 421 KSVDSNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPN 480
           KSVDSNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPN
Sbjct: 421 KSVDSNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPN 480

Query: 481 TSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLDADD 540
           TSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLDADD
Sbjct: 481 TSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLDADD 540

Query: 541 DDSIIVTIIPRLGAGLVSIAEIKIEYGD 569
           DDSIIVTIIPRLGAGLVSIAEIKIEYGD
Sbjct: 541 DDSIIVTIIPRLGAGLVSIAEIKIEYGD 568

BLAST of CmaCh13G006570 vs. NCBI nr
Match: XP_023519283.1 (polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1099.0 bits (2841), Expect = 0.0e+00
Identity = 530/568 (93.31%), Postives = 553/568 (97.36%), Query Frame = 0

Query: 1   MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAANGKIDRRDVLIGIGAGGIYGAS 60
           MASLPPPALSTA FRPSS   TSKYLP NAAR+LCSAA+GKIDRRDVLIGIGAGG+YGAS
Sbjct: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 60

Query: 61  AFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120
           AFTKDSTFALAAPVQTPNISKCGPPDLPPG+DPTNCCPPP+ SIVDF PPAPGKLRVRPA
Sbjct: 61  AFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIVDFLPPAPGKLRVRPA 120

Query: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHLADQSYIDKYKRAVELMKGLP DDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGM+MPAMYAD NSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 240

Query: 241 HLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300
           HLPPTL+DLDFDGVDSNIG+NAQIR+NLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTLIDLDFDGVDSNIGNNAQIRTNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360
           GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSN+DRMWSVWKTLGGRR+
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 360

Query: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ 420
           DLQYPEWLDASFIFYNEK +AVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ
Sbjct: 361 DLQYPEWLDASFIFYNEKGEAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ 420

Query: 421 KSVDSNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPN 480
           KSVDSNVPSRRVKP +MK PVT++SA+STEVKRGKKSR RKEKDEEEEILVIKGIEFDPN
Sbjct: 421 KSVDSNVPSRRVKPGRMKFPVTVESAVSTEVKRGKKSRSRKEKDEEEEILVIKGIEFDPN 480

Query: 481 TSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLDADD 540
           T+IKLD+YINDED+REIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGI+EVLEDL+ADD
Sbjct: 481 TAIKLDVYINDEDEREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGITEVLEDLEADD 540

Query: 541 DDSIIVTIIPRLGAGLVSIAEIKIEYGD 569
           DDSIIVTIIP LGAGLVSIA+I +EYGD
Sbjct: 541 DDSIIVTIIPSLGAGLVSIADITLEYGD 568

BLAST of CmaCh13G006570 vs. NCBI nr
Match: KAG6584007.1 (hypothetical protein SDJN03_19939, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 531/568 (93.49%), Postives = 550/568 (96.83%), Query Frame = 0

Query: 1   MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAANGKIDRRDVLIGIGAGGIYGAS 60
           MASLPPPALSTA FRPSS   TSKYLP NAAR+LCSAA+GKIDRRDVLIGIGAGG+YGAS
Sbjct: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 60

Query: 61  AFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120
           AFTKDSTFALAAPVQTPNISKCGPPDLPPG+DPTNCCPPP+ SI+DF PPAPGKLRVRPA
Sbjct: 61  AFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA 120

Query: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHLADQSYIDKYKRAVELMKGLP DDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELI DPTFALPFWNYDAPGGM+MPAMYAD NSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIEDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 240

Query: 241 HLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300
           HLPPT +DLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360
           GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSN+DRMWSVWKTLGGRR+
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 360

Query: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ 420
           DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTK LGYIYQDVELPWLETRPTPRRALRAQ
Sbjct: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKTLGYIYQDVELPWLETRPTPRRALRAQ 420

Query: 421 KSVDSNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPN 480
           KSVDSNVPSRRVKP ++K PVT+KSA+STEVKR KKSR +KEK+EEEEILVIKGIEFDPN
Sbjct: 421 KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN 480

Query: 481 TSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLDADD 540
           T+IKLD+YINDEDD EIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDL+ADD
Sbjct: 481 TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD 540

Query: 541 DDSIIVTIIPRLGAGLVSIAEIKIEYGD 569
           DDSIIVTIIPRLGAGLVSIAEI IEYGD
Sbjct: 541 DDSIIVTIIPRLGAGLVSIAEITIEYGD 568

BLAST of CmaCh13G006570 vs. NCBI nr
Match: XP_022927081.1 (polyphenol oxidase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 530/568 (93.31%), Postives = 551/568 (97.01%), Query Frame = 0

Query: 1   MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAANGKIDRRDVLIGIGAGGIYGAS 60
           MASLPPPALSTA FRPSS   TSKYLP NAAR+LCSAA+GKIDRRDVLIGIGAGG+YGAS
Sbjct: 1   MASLPPPALSTAAFRPSSSLRTSKYLPINAARILCSAADGKIDRRDVLIGIGAGGLYGAS 60

Query: 61  AFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120
           +FTKDSTFALAAPVQTPNISKCGPPDLPPG+DPTNCCPPP+ SI+DF PPAPGKLRVRPA
Sbjct: 61  SFTKDSTFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGKLRVRPA 120

Query: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHLADQSYIDKYKRAVELMKGLP DDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGM+MPAMYAD NSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYDQLRNQN 240

Query: 241 HLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300
           HLPPT +DLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360
           GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSN+DRMWSVWKTLGGRR+
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKTLGGRRQ 360

Query: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ 420
           DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVEL WLETRPTPRRALRAQ
Sbjct: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPRRALRAQ 420

Query: 421 KSVDSNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPN 480
           KSVDSNVPSRRVKP ++K PVT+KSA+STEVKR KKSR +KEK+EEEEILVIKGIEFDPN
Sbjct: 421 KSVDSNVPSRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKEEEEEILVIKGIEFDPN 480

Query: 481 TSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLDADD 540
           T+IKLD+YINDEDD EIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDL+ADD
Sbjct: 481 TAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLEADD 540

Query: 541 DDSIIVTIIPRLGAGLVSIAEIKIEYGD 569
           DDSIIVTIIPRLGAGLVSIAEI +EYGD
Sbjct: 541 DDSIIVTIIPRLGAGLVSIAEITMEYGD 568

BLAST of CmaCh13G006570 vs. NCBI nr
Match: XP_023001711.1 (polyphenol oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1087.8 bits (2812), Expect = 0.0e+00
Identity = 527/568 (92.78%), Postives = 546/568 (96.13%), Query Frame = 0

Query: 1   MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAANGKIDRRDVLIGIGAGGIYGAS 60
           M SL  PALST TFRPSSRPPTSKYLPNNAARVLCSAA+GKIDRRDVLIGIGAGGIYGAS
Sbjct: 1   MGSLSSPALSTTTFRPSSRPPTSKYLPNNAARVLCSAADGKIDRRDVLIGIGAGGIYGAS 60

Query: 61  AFTKDSTFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRPPAPGKLRVRPA 120
           AFTKDSTFALAAPVQTPNISKC PPDLP G+DPTNCCPPPSPSIVDFRPP PGKLRVRPA
Sbjct: 61  AFTKDSTFALAAPVQTPNISKCSPPDLPHGADPTNCCPPPSPSIVDFRPPVPGKLRVRPA 120

Query: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVGFPVEVQVHNSW 180
           AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQAN+HCAYCNGGYDQVGFPVEVQVHNSW
Sbjct: 121 AHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANIHCAYCNGGYDQVGFPVEVQVHNSW 180

Query: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240
           LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN
Sbjct: 181 LFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHNSSLYDQLRNQN 240

Query: 241 HLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGDEPSPGG 300
           HLPPTLMDLDFDGVD  IGD+A I+SNLRIMYRQMVSNS TPKLFFGSAYVAGDEPSPGG
Sbjct: 241 HLPPTLMDLDFDGVDPTIGDDALIKSNLRIMYRQMVSNSVTPKLFFGSAYVAGDEPSPGG 300

Query: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360
           GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR
Sbjct: 301 GSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMWSVWKTLGGRRR 360

Query: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPRRALRAQ 420
           DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTP RALRAQ
Sbjct: 361 DLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPCRALRAQ 420

Query: 421 KSVDSNVPSRRVKPPKMKLPVTLKSAISTEVKRGKKSRGRKEKDEEEEILVIKGIEFDPN 480
           KSVDSNVPSRRVKPPKMK PVTLKSAISTEVKRGKKSR +KEK+EEEEILVI+GI+ DPN
Sbjct: 421 KSVDSNVPSRRVKPPKMKFPVTLKSAISTEVKRGKKSRSKKEKEEEEEILVIEGIKLDPN 480

Query: 481 TSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKKKVKTGLRLGISEVLEDLDADD 540
             +K DIYINDED++E R D +EFAGSFVNVPHKHGHKKK+KTGLRLGI+++LEDLDAD 
Sbjct: 481 MLVKFDIYINDEDEKENRADISEFAGSFVNVPHKHGHKKKMKTGLRLGITQLLEDLDADC 540

Query: 541 DDSIIVTIIPRLGAGLVSIAEIKIEYGD 569
           DDSIIVT+IPRLGAGLVSIA+IKIEYGD
Sbjct: 541 DDSIIVTLIPRLGAGLVSIAKIKIEYGD 568

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P433099.6e-20258.05Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1[more]
Q062151.1e-17356.25Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1[more]
P433117.0e-16852.04Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1[more]
Q9FRX62.2e-16154.73Aureusidin synthase OS=Antirrhinum majus OX=4151 GN=AS1 PE=1 SV=1[more]
Q9ZP194.5e-15954.47Polyphenol oxidase I, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KRC00.0e+00100.00polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114957... [more]
A0A6J1EGP50.0e+0093.31polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A6J1KJD30.0e+0092.78polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114957... [more]
A0A6J1EMV75.9e-28785.92polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
A0A1L5JHU32.9e-25774.07Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023001713.10.0e+00100.00polyphenol oxidase, chloroplastic-like [Cucurbita maxima][more]
XP_023519283.10.0e+0093.31polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAG6584007.10.0e+0093.49hypothetical protein SDJN03_19939, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022927081.10.0e+0093.31polyphenol oxidase, chloroplastic-like [Cucurbita moschata][more]
XP_023001711.10.0e+0092.78polyphenol oxidase, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002227Tyrosinase copper-binding domainPRINTSPR00092TYROSINASEcoord: 177..194
score: 58.96
coord: 334..352
score: 57.57
coord: 208..213
score: 55.1
coord: 307..318
score: 41.33
IPR002227Tyrosinase copper-binding domainPFAMPF00264Tyrosinasecoord: 147..354
e-value: 2.0E-33
score: 116.6
IPR002227Tyrosinase copper-binding domainPROSITEPS00497TYROSINASE_1coord: 177..194
IPR002227Tyrosinase copper-binding domainPROSITEPS00498TYROSINASE_2coord: 335..346
IPR016213Polyphenol oxidasePIRSFPIRSF000290PPO_plantcoord: 21..568
e-value: 1.7E-248
score: 824.2
IPR008922Uncharacterised domain, di-copper centreGENE3D1.10.1280.10coord: 72..408
e-value: 1.3E-120
score: 404.9
IPR008922Uncharacterised domain, di-copper centreSUPERFAMILY48056Di-copper centre-containing domaincoord: 78..407
IPR022740Polyphenol oxidase, C-terminalPFAMPF12143PPO1_KFDVcoord: 440..566
e-value: 1.2E-47
score: 161.3
IPR022739Polyphenol oxidase, central domainPFAMPF12142PPO1_DWLcoord: 361..412
e-value: 1.0E-22
score: 79.6
NoneNo IPR availablePIRSRPIRSR000290-1PIRSR000290-1coord: 72..567
e-value: 3.4E-231
score: 766.2
NoneNo IPR availablePIRSRPIRSR000290-2PIRSR000290-2coord: 72..567
e-value: 3.4E-231
score: 766.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 10..25
NoneNo IPR availablePANTHERPTHR11474TYROSINASE FAMILY MEMBERcoord: 26..566
NoneNo IPR availablePANTHERPTHR11474:SF94POLYPHENOL OXIDASE, CHLOROPLASTIC-LIKEcoord: 26..566

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh13G006570.1CmaCh13G006570.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046148 pigment biosynthetic process
molecular_function GO:0004097 catechol oxidase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity