CmaCh13G005850 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh13G005850
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionWRKY protein
LocationCma_Chr13: 5832711 .. 5834151 (-)
RNA-Seq ExpressionCmaCh13G005850
SyntenyCmaCh13G005850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTGCCCCTTTATTCTTCTTCCTCCTCTTCTTCAGAACCCTCTGATACAAACTTTTCCAACAACCTTAAAAAACAGCCACCCATCTACGACGGCCGGCCATATATAGTAATATGGACGATGTTACCTTCAACAATAACCACCAATTCTATTCTGTTGATCTCTTAGAAGATAGCGACGAATTCAAGGCAGCCCCTGATTCTCCTCCTCCTCCTCCTCCTCCTCCTCTGCCTCCTAATTCCACCGCCGCCGCCAAGAAGGGCAGGTCAGTTGGTGAATTCAGATTGATTTTATTTTTTGTGTATTTATTTTTTTTAAAAATTTGATGGTGTGTAGAAGAGGGATGAAGAAGAAAGTGGTGTCGGTGCGGATTAATGGCGACGGCCCAAGGAATTCCTCTGGTTCTACGACTCCACCTTCTGATTCTTGGGCCTGGAGAAAGTACGGCCAAAAGCCCATCAAGGGCTCCCCTTATCCCAGGTGATCTATTTTATTAAAGCTTGATTTGGAGGAAATTGATGGGTTTTGATTGGATTTGGGTTTAATTTTGTAGGGCGTATTATAGGTGTAGTAGCTCCAAGGGATGTCCGGCGAGGAAGCAAGTAGAGAGGAACCGGCTTGATCCCACCATGTTGCTCATTACTTATTCCTGCGAGCACAACCATTCCGGTCCGGTTTCCAGAAACAACAATCACCACCAAGCCGCCGCCGTGAAACCCACTTCGCCAGAGACTGTTCCGGTTCATCCAGAACCGGAACCGGAGGCGGAAGAGAAATTTGTGGAGTCGTTAGAGGACCAGTTCAGCTGGTTTGGGGAAATGGAAACCACGTCGTCCACAGTGCTCGAAAGCTCCATTTTCTCGGGGAGATCATCGAGCGGAGTGGGAGATTCGATTTCCAGTGACGTGGCAATGCCTTTTCCGATGGGGGATGATGACGTGGACGACTCTCTCTTTGCGGATCTCGGGGAGTTGCCGGAGTGCTCTGTCGTGTTCCGACACGGCGGAGGCCCGGGATTACCGGTCGATGAACAACCGGCTACAAGCCAGCGGCGGATCACTCCTTGGTGCGGAGCCACCACTTGATATGTGCGGCGCGGCCTTCTCTTTGTTTGTATACTTCTAGGAGCATCGTCCGCCATTAATGCCGGGAAAGTTTCTTTCTTCTCTTCTTTTCTTTTTAAATTATAATAATTATTTTTTTTTTCTAAAACTTGGTTATATAACAAAAGCAAATTCATGTTTCGTTTCATTTCATCCCAACTTAAAATTACCATTTTAATCACGATTTTGATTTGTGTGGAACATTTTAATTAATCACGGATTAAAATTTTTCAAAATATGGTTGTGAACATTAACAATTTTAATCTTAAATCGTTACATTAAAATAAGATCAGCCCCACCGGATTAGAGCTCCATAAATAAATACCTCTACATGCTTAG

mRNA sequence

AATTGCCCCTTTATTCTTCTTCCTCCTCTTCTTCAGAACCCTCTGATACAAACTTTTCCAACAACCTTAAAAAACAGCCACCCATCTACGACGGCCGGCCATATATAGTAATATGGACGATGTTACCTTCAACAATAACCACCAATTCTATTCTGTTGATCTCTTAGAAGATAGCGACGAATTCAAGGCAGCCCCTGATTCTCCTCCTCCTCCTCCTCCTCCTCCTCTGCCTCCTAATTCCACCGCCGCCGCCAAGAAGGGCAGAAGAGGGATGAAGAAGAAAGTGGTGTCGGTGCGGATTAATGGCGACGGCCCAAGGAATTCCTCTGGTTCTACGACTCCACCTTCTGATTCTTGGGCCTGGAGAAAGTACGGCCAAAAGCCCATCAAGGGCTCCCCTTATCCCAGGGCGTATTATAGGTGTAGTAGCTCCAAGGGATGTCCGGCGAGGAAGCAAGTAGAGAGGAACCGGCTTGATCCCACCATGTTGCTCATTACTTATTCCTGCGAGCACAACCATTCCGGTCCGGTTTCCAGAAACAACAATCACCACCAAGCCGCCGCCGTGAAACCCACTTCGCCAGAGACTGTTCCGGTTCATCCAGAACCGGAACCGGAGGCGGAAGAGAAATTTGTGGAGTCGTTAGAGGACCAGTTCAGCTGGTTTGGGGAAATGGAAACCACGTCGTCCACAGTGCTCGAAAGCTCCATTTTCTCGGGGAGATCATCGAGCGGAGTGGGAGATTCGATTTCCAGTGACGTGGCAATGCCTTTTCCGATGGGGGATGATGACGTGGACGACTCTCTCTTTGCGGATCTCGGGGAGTTGCCGGAGTGCTCTGTCGTGTTCCGACACGGCGGAGGCCCGGGATTACCGGTCGATGAACAACCGGCTACAAGCCAGCGGCGGATCACTCCTTGGTGCGGAGCCACCACTTGATATGTGCGGCGCGGCCTTCTCTTTGTTTGTATACTTCTAGGAGCATCGTCCGCCATTAATGCCGGGAAAGTTTCTTTCTTCTCTTCTTTTCTTTTTAAATTATAATAATTATTTTTTTTTTCTAAAACTTGGTTATATAACAAAAGCAAATTCATGTTTCGTTTCATTTCATCCCAACTTAAAATTACCATTTTAATCACGATTTTGATTTGTGTGGAACATTTTAATTAATCACGGATTAAAATTTTTCAAAATATGGTTGTGAACATTAACAATTTTAATCTTAAATCGTTACATTAAAATAAGATCAGCCCCACCGGATTAGAGCTCCATAAATAAATACCTCTACATGCTTAG

Coding sequence (CDS)

ATGGACGATGTTACCTTCAACAATAACCACCAATTCTATTCTGTTGATCTCTTAGAAGATAGCGACGAATTCAAGGCAGCCCCTGATTCTCCTCCTCCTCCTCCTCCTCCTCCTCTGCCTCCTAATTCCACCGCCGCCGCCAAGAAGGGCAGAAGAGGGATGAAGAAGAAAGTGGTGTCGGTGCGGATTAATGGCGACGGCCCAAGGAATTCCTCTGGTTCTACGACTCCACCTTCTGATTCTTGGGCCTGGAGAAAGTACGGCCAAAAGCCCATCAAGGGCTCCCCTTATCCCAGGGCGTATTATAGGTGTAGTAGCTCCAAGGGATGTCCGGCGAGGAAGCAAGTAGAGAGGAACCGGCTTGATCCCACCATGTTGCTCATTACTTATTCCTGCGAGCACAACCATTCCGGTCCGGTTTCCAGAAACAACAATCACCACCAAGCCGCCGCCGTGAAACCCACTTCGCCAGAGACTGTTCCGGTTCATCCAGAACCGGAACCGGAGGCGGAAGAGAAATTTGTGGAGTCGTTAGAGGACCAGTTCAGCTGGTTTGGGGAAATGGAAACCACGTCGTCCACAGTGCTCGAAAGCTCCATTTTCTCGGGGAGATCATCGAGCGGAGTGGGAGATTCGATTTCCAGTGACGTGGCAATGCCTTTTCCGATGGGGGATGATGACGTGGACGACTCTCTCTTTGCGGATCTCGGGGAGTTGCCGGAGTGCTCTGTCGTGTTCCGACACGGCGGAGGCCCGGGATTACCGGTCGATGAACAACCGGCTACAAGCCAGCGGCGGATCACTCCTTGGTGCGGAGCCACCACTTGA

Protein sequence

MDDVTFNNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVSVRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLEDQFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELPECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT
Homology
BLAST of CmaCh13G005850 vs. ExPASy Swiss-Prot
Match: Q9LP56 (Probable WRKY transcription factor 65 OS=Arabidopsis thaliana OX=3702 GN=WRKY65 PE=2 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.3e-55
Identity = 124/218 (56.88%), Postives = 150/218 (68.81%), Query Frame = 0

Query: 43  STAAAKKGRRGMKKKVVSVRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYY 102
           S+ + K+ RR ++K+VV+V +         G TTPPSDSWAWRKYGQKPIKGSPYPR YY
Sbjct: 38  SSHSPKRSRRSVEKRVVNVPMKEMEGSRHKGDTTPPSDSWAWRKYGQKPIKGSPYPRGYY 97

Query: 103 RCSSSKGCPARKQVERNRLDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPV 162
           RCSS+KGCPARKQVER+R DPTM+LITY+ EHNH  P++ +  +      KP        
Sbjct: 98  RCSSTKGCPARKQVERSRDDPTMILITYTSEHNHPWPLTSSTRN----GPKPKPEPKPEP 157

Query: 163 HPEPEPEAEE---KFV---------ESLEDQFSWFGEMETTSSTVLESSIFSGRSSSGVG 222
            PE EPEAEE   KF+          S  D+F+WF EMETTSST+LES IFS    + V 
Sbjct: 158 EPEVEPEAEEEDNKFMVLGRGIETTPSCVDEFAWFTEMETTSSTILESPIFSSEKKTAV- 217

Query: 223 DSISSDVAMPFPMGDDDVDDSLFADLGELPECSVVFRH 249
            S + DVA+ FPMG++  D+SLFADLGELPECSVVFRH
Sbjct: 218 -SGADDVAVFFPMGEE--DESLFADLGELPECSVVFRH 247

BLAST of CmaCh13G005850 vs. ExPASy Swiss-Prot
Match: Q93WV5 (Probable WRKY transcription factor 69 OS=Arabidopsis thaliana OX=3702 GN=WRKY69 PE=2 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 4.3e-38
Identity = 109/269 (40.52%), Postives = 149/269 (55.39%), Query Frame = 0

Query: 20  DSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVSVRINGDGPRNSSGSTTPPS 79
           D +E +   D  P  P        +    K RR ++K+VVSV I       S G   PPS
Sbjct: 11  DDEEDETYNDVVPESPSSCEDSKISKPTPKKRRNVEKRVVSVPIADVEGSKSRGEVYPPS 70

Query: 80  DSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDPTMLLITYSCEHNHSGP 139
           DSWAWRKYGQKPIKGSPYPR YYRCSSSKGCPARKQVER+R+DP+ L+ITY+C+HNH  P
Sbjct: 71  DSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLMITYACDHNHPFP 130

Query: 140 VSRNN--NHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLEDQFSWFGEMETTSSTVLE 199
            S  N  +HH+++ V  T+ +      E E E EE  V + E+  +   ++    S +L 
Sbjct: 131 SSSANTKSHHRSSVVLKTAKK----EEEYEEEEEELTVTAAEEPPAGL-DLSHVDSPLLL 190

Query: 200 SSIFSG------------RSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELPECSVV 259
              +S              SSSG  + +   +   F +G ++ D+SLF DLG+LP+C+ V
Sbjct: 191 GGCYSEIGEFGWFYDASISSSSGSSNFLDVTLERGFSVGQEE-DESLFGDLGDLPDCASV 250

Query: 260 FRHGGGPGLPVDEQPATSQRRITPWCGAT 275
           FR G    +  +EQ         P+C ++
Sbjct: 251 FRRG---TVATEEQHRRCDFGAIPFCDSS 270

BLAST of CmaCh13G005850 vs. ExPASy Swiss-Prot
Match: Q9SA80 (Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 PE=2 SV=2)

HSP 1 Score: 125.6 bits (314), Expect = 8.9e-28
Identity = 92/216 (42.59%), Postives = 109/216 (50.46%), Query Frame = 0

Query: 49  KGRRGMKKKVVSVRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSK 108
           K R+   KKVV +         SSG    PSD WAWRKYGQKPIKGSP+PR YYRCSSSK
Sbjct: 188 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPFPRGYYRCSSSK 247

Query: 109 GCPARKQVERNRLDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEP-E 168
           GC ARKQVER+R DP ML+ITY+ EHNH  P+ RN     A A    S  +   +P P +
Sbjct: 248 GCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRN-----ALAGSTRSSTSSSSNPNPSK 307

Query: 169 PEAEEKFVESLEDQFSWFGEMETTSSTVLESSIFSGRSS-----SGVGDSISSDVAMPFP 228
           P        S+  Q + +    TT    L SS              V D   + +A   P
Sbjct: 308 PSTANVNSSSIGSQNTIYLPSSTTPPPTLSSSAIKDERGDDMELENVDDDDDNQIAPYRP 367

Query: 229 MGDD--DVDDSLFADLGEL--PECSVVFRHG-GGPG 254
              D     D  FADL EL     S++  HG GG G
Sbjct: 368 ELHDHQHQPDDFFADLEELEGDSLSMLLSHGCGGDG 397

BLAST of CmaCh13G005850 vs. ExPASy Swiss-Prot
Match: O64747 (Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 8.9e-28
Identity = 62/95 (65.26%), Postives = 67/95 (70.53%), Query Frame = 0

Query: 49  KGRRGMKKKVVSVRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSK 108
           K R+   KKVV +         SSG    PSD WAWRKYGQKPIKGSPYPR YYRCSSSK
Sbjct: 186 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 245

Query: 109 GCPARKQVERNRLDPTMLLITYSCEHNHSGPVSRN 144
           GC ARKQVER+R DP ML+ITY+ EHNH  P  RN
Sbjct: 246 GCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRN 279

BLAST of CmaCh13G005850 vs. ExPASy Swiss-Prot
Match: O04609 (WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.1e-25
Identity = 77/193 (39.90%), Postives = 100/193 (51.81%), Query Frame = 0

Query: 40  PPNSTAAAKKGRRGMKKKVVSVRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPR 99
           P  S  +  K R+   KKV  V            +    SD WAWRKYGQKPIKGSPYPR
Sbjct: 100 PSGSNTSRSKRRKIQHKKVCHV-----------AAEALNSDVWAWRKYGQKPIKGSPYPR 159

Query: 100 AYYRCSSSKGCPARKQVERNRLDPTMLLITYSCEHNHSGPVSRNN--------NHHQAAA 159
            YYRCS+SKGC ARKQVERNR DP M ++TY+ EHNH  P  RN+           Q  +
Sbjct: 160 GYYRCSTSKGCLARKQVERNRSDPKMFIVTYTAEHNHPAPTHRNSLAGSTRQKPSDQQTS 219

Query: 160 VKPTSPETVPVHPEPEPEAEEKFVESLEDQFSWFGEME------TTSSTVLESSIFSGRS 219
             PT+  T+  +      + ++FV  +ED  +  G+++      + S TV+    F G  
Sbjct: 220 KSPTT--TIATYSSSPVTSADEFVLPVEDHLA-VGDLDGEEDLLSLSDTVVSDDFFDGLE 278

BLAST of CmaCh13G005850 vs. ExPASy TrEMBL
Match: A0A6J1KJW5 (probable WRKY transcription factor 65 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495268 PE=4 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 3.4e-155
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0

Query: 1   MDDVTFNNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVS 60
           MDDVTFNNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVS
Sbjct: 1   MDDVTFNNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVS 60

Query: 61  VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNR 120
           VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNR
Sbjct: 61  VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNR 120

Query: 121 LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLED 180
           LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLED
Sbjct: 121 LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLED 180

Query: 181 QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP 240
           QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP
Sbjct: 181 QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP 240

Query: 241 ECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT 276
           ECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT
Sbjct: 241 ECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT 275

BLAST of CmaCh13G005850 vs. ExPASy TrEMBL
Match: A0A6J1KF84 (probable WRKY transcription factor 65 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495268 PE=4 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 8.7e-151
Identity = 271/275 (98.55%), Postives = 271/275 (98.55%), Query Frame = 0

Query: 1   MDDVTFNNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVS 60
           MDDVTFNNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVS
Sbjct: 1   MDDVTFNNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVS 60

Query: 61  VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNR 120
           VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYP    RCSSSKGCPARKQVERNR
Sbjct: 61  VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYP----RCSSSKGCPARKQVERNR 120

Query: 121 LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLED 180
           LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLED
Sbjct: 121 LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLED 180

Query: 181 QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP 240
           QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP
Sbjct: 181 QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP 240

Query: 241 ECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT 276
           ECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT
Sbjct: 241 ECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT 271

BLAST of CmaCh13G005850 vs. ExPASy TrEMBL
Match: A0A6J1EGX5 (probable WRKY transcription factor 65 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434096 PE=4 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 9.0e-148
Identity = 270/275 (98.18%), Postives = 270/275 (98.18%), Query Frame = 0

Query: 1   MDDVTFNNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVS 60
           MDDVTFNNNHQFYSVDLLEDSDEFKAAPDS PPPPPPPLPPNST AAKKGRRGMKKKVVS
Sbjct: 1   MDDVTFNNNHQFYSVDLLEDSDEFKAAPDS-PPPPPPPLPPNST-AAKKGRRGMKKKVVS 60

Query: 61  VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNR 120
           VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNR
Sbjct: 61  VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNR 120

Query: 121 LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLED 180
           LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVH  PEPEAEEKFVESLED
Sbjct: 121 LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVH--PEPEAEEKFVESLED 180

Query: 181 QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP 240
           QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP
Sbjct: 181 QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP 240

Query: 241 ECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT 276
           ECSVVFRHGGGP LPVDEQPATSQRRITPWCGATT
Sbjct: 241 ECSVVFRHGGGPELPVDEQPATSQRRITPWCGATT 271

BLAST of CmaCh13G005850 vs. ExPASy TrEMBL
Match: A0A6J1EGD7 (probable WRKY transcription factor 65 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111434096 PE=4 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 2.3e-143
Identity = 266/275 (96.73%), Postives = 266/275 (96.73%), Query Frame = 0

Query: 1   MDDVTFNNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVS 60
           MDDVTFNNNHQFYSVDLLEDSDEFKAAPDS PPPPPPPLPPNST AAKKGRRGMKKKVVS
Sbjct: 1   MDDVTFNNNHQFYSVDLLEDSDEFKAAPDS-PPPPPPPLPPNST-AAKKGRRGMKKKVVS 60

Query: 61  VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNR 120
           VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYP    RCSSSKGCPARKQVERNR
Sbjct: 61  VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYP----RCSSSKGCPARKQVERNR 120

Query: 121 LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLED 180
           LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVH  PEPEAEEKFVESLED
Sbjct: 121 LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVH--PEPEAEEKFVESLED 180

Query: 181 QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP 240
           QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP
Sbjct: 181 QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP 240

Query: 241 ECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT 276
           ECSVVFRHGGGP LPVDEQPATSQRRITPWCGATT
Sbjct: 241 ECSVVFRHGGGPELPVDEQPATSQRRITPWCGATT 267

BLAST of CmaCh13G005850 vs. ExPASy TrEMBL
Match: A0A6J1EK89 (probable WRKY transcription factor 65 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111434096 PE=4 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 1.6e-136
Identity = 257/275 (93.45%), Postives = 257/275 (93.45%), Query Frame = 0

Query: 1   MDDVTFNNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVS 60
           MDDVTFNNNHQFYSVDLLEDSDEFKAAPDS PPPPPPPLPPNST AAKKGRRGMKKKVVS
Sbjct: 1   MDDVTFNNNHQFYSVDLLEDSDEFKAAPDS-PPPPPPPLPPNST-AAKKGRRGMKKKVVS 60

Query: 61  VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNR 120
           VRINGDGPRNSSGSTTPPSDSWAWRK             AYYRCSSSKGCPARKQVERNR
Sbjct: 61  VRINGDGPRNSSGSTTPPSDSWAWRK-------------AYYRCSSSKGCPARKQVERNR 120

Query: 121 LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLED 180
           LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVH  PEPEAEEKFVESLED
Sbjct: 121 LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVH--PEPEAEEKFVESLED 180

Query: 181 QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP 240
           QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP
Sbjct: 181 QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP 240

Query: 241 ECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT 276
           ECSVVFRHGGGP LPVDEQPATSQRRITPWCGATT
Sbjct: 241 ECSVVFRHGGGPELPVDEQPATSQRRITPWCGATT 258

BLAST of CmaCh13G005850 vs. NCBI nr
Match: XP_023001000.1 (probable WRKY transcription factor 65 isoform X1 [Cucurbita maxima])

HSP 1 Score: 557.4 bits (1435), Expect = 7.0e-155
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0

Query: 1   MDDVTFNNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVS 60
           MDDVTFNNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVS
Sbjct: 1   MDDVTFNNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVS 60

Query: 61  VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNR 120
           VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNR
Sbjct: 61  VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNR 120

Query: 121 LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLED 180
           LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLED
Sbjct: 121 LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLED 180

Query: 181 QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP 240
           QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP
Sbjct: 181 QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP 240

Query: 241 ECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT 276
           ECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT
Sbjct: 241 ECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT 275

BLAST of CmaCh13G005850 vs. NCBI nr
Match: KAG6583943.1 (putative WRKY transcription factor 65, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 548.5 bits (1412), Expect = 3.3e-152
Identity = 273/278 (98.20%), Postives = 274/278 (98.56%), Query Frame = 0

Query: 1   MDDVTFNNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNST---AAAKKGRRGMKKK 60
           MDDVTFNNNHQFYSVDLLEDS+EFKAAPDSPPPPPPPPLPPNST   AAAKKGRRGMKKK
Sbjct: 1   MDDVTFNNNHQFYSVDLLEDSEEFKAAPDSPPPPPPPPLPPNSTAAAAAAKKGRRGMKKK 60

Query: 61  VVSVRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVE 120
           VVSVRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVE
Sbjct: 61  VVSVRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVE 120

Query: 121 RNRLDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVES 180
           RNRLDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVES
Sbjct: 121 RNRLDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVES 180

Query: 181 LEDQFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLG 240
           LEDQFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLG
Sbjct: 181 LEDQFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLG 240

Query: 241 ELPECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT 276
           ELPECSVVFRHGGGPGLPVDEQP TSQRRITPWCGATT
Sbjct: 241 ELPECSVVFRHGGGPGLPVDEQPPTSQRRITPWCGATT 278

BLAST of CmaCh13G005850 vs. NCBI nr
Match: XP_023001002.1 (probable WRKY transcription factor 65 isoform X2 [Cucurbita maxima])

HSP 1 Score: 542.7 bits (1397), Expect = 1.8e-150
Identity = 271/275 (98.55%), Postives = 271/275 (98.55%), Query Frame = 0

Query: 1   MDDVTFNNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVS 60
           MDDVTFNNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVS
Sbjct: 1   MDDVTFNNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVS 60

Query: 61  VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNR 120
           VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYP    RCSSSKGCPARKQVERNR
Sbjct: 61  VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYP----RCSSSKGCPARKQVERNR 120

Query: 121 LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLED 180
           LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLED
Sbjct: 121 LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLED 180

Query: 181 QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP 240
           QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP
Sbjct: 181 QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP 240

Query: 241 ECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT 276
           ECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT
Sbjct: 241 ECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT 271

BLAST of CmaCh13G005850 vs. NCBI nr
Match: KAG7019560.1 (putative WRKY transcription factor 65, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 541.6 bits (1394), Expect = 4.0e-150
Identity = 271/276 (98.19%), Postives = 272/276 (98.55%), Query Frame = 0

Query: 1   MDDVTFNNNHQFYSVDLLEDSDEFKAAPDS-PPPPPPPPLPPNSTAAAKKGRRGMKKKVV 60
           MDDVTFNNNHQFYSVDLLEDS+EFKAAPDS PPPPPPPPLPPNST A KKGRRGMKKKVV
Sbjct: 1   MDDVTFNNNHQFYSVDLLEDSEEFKAAPDSPPPPPPPPPLPPNST-ATKKGRRGMKKKVV 60

Query: 61  SVRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERN 120
           SVRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERN
Sbjct: 61  SVRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERN 120

Query: 121 RLDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLE 180
           RLDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLE
Sbjct: 121 RLDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLE 180

Query: 181 DQFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGEL 240
           DQFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGEL
Sbjct: 181 DQFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGEL 240

Query: 241 PECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT 276
           PECSVVFRHGGGPGLPVDEQP TSQRRITPWCGATT
Sbjct: 241 PECSVVFRHGGGPGLPVDEQPPTSQRRITPWCGATT 275

BLAST of CmaCh13G005850 vs. NCBI nr
Match: XP_022927161.1 (probable WRKY transcription factor 65 isoform X1 [Cucurbita moschata])

HSP 1 Score: 532.7 bits (1371), Expect = 1.9e-147
Identity = 270/275 (98.18%), Postives = 270/275 (98.18%), Query Frame = 0

Query: 1   MDDVTFNNNHQFYSVDLLEDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVS 60
           MDDVTFNNNHQFYSVDLLEDSDEFKAAPDS PPPPPPPLPPNST AAKKGRRGMKKKVVS
Sbjct: 1   MDDVTFNNNHQFYSVDLLEDSDEFKAAPDS-PPPPPPPLPPNST-AAKKGRRGMKKKVVS 60

Query: 61  VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNR 120
           VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNR
Sbjct: 61  VRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNR 120

Query: 121 LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLED 180
           LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVH  PEPEAEEKFVESLED
Sbjct: 121 LDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVH--PEPEAEEKFVESLED 180

Query: 181 QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP 240
           QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP
Sbjct: 181 QFSWFGEMETTSSTVLESSIFSGRSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELP 240

Query: 241 ECSVVFRHGGGPGLPVDEQPATSQRRITPWCGATT 276
           ECSVVFRHGGGP LPVDEQPATSQRRITPWCGATT
Sbjct: 241 ECSVVFRHGGGPELPVDEQPATSQRRITPWCGATT 271

BLAST of CmaCh13G005850 vs. TAIR 10
Match: AT1G29280.1 (WRKY DNA-binding protein 65 )

HSP 1 Score: 218.0 bits (554), Expect = 9.4e-57
Identity = 124/218 (56.88%), Postives = 150/218 (68.81%), Query Frame = 0

Query: 43  STAAAKKGRRGMKKKVVSVRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYY 102
           S+ + K+ RR ++K+VV+V +         G TTPPSDSWAWRKYGQKPIKGSPYPR YY
Sbjct: 38  SSHSPKRSRRSVEKRVVNVPMKEMEGSRHKGDTTPPSDSWAWRKYGQKPIKGSPYPRGYY 97

Query: 103 RCSSSKGCPARKQVERNRLDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPV 162
           RCSS+KGCPARKQVER+R DPTM+LITY+ EHNH  P++ +  +      KP        
Sbjct: 98  RCSSTKGCPARKQVERSRDDPTMILITYTSEHNHPWPLTSSTRN----GPKPKPEPKPEP 157

Query: 163 HPEPEPEAEE---KFV---------ESLEDQFSWFGEMETTSSTVLESSIFSGRSSSGVG 222
            PE EPEAEE   KF+          S  D+F+WF EMETTSST+LES IFS    + V 
Sbjct: 158 EPEVEPEAEEEDNKFMVLGRGIETTPSCVDEFAWFTEMETTSSTILESPIFSSEKKTAV- 217

Query: 223 DSISSDVAMPFPMGDDDVDDSLFADLGELPECSVVFRH 249
            S + DVA+ FPMG++  D+SLFADLGELPECSVVFRH
Sbjct: 218 -SGADDVAVFFPMGEE--DESLFADLGELPECSVVFRH 247

BLAST of CmaCh13G005850 vs. TAIR 10
Match: AT3G58710.1 (WRKY DNA-binding protein 69 )

HSP 1 Score: 162.9 bits (411), Expect = 3.6e-40
Identity = 111/270 (41.11%), Postives = 150/270 (55.56%), Query Frame = 0

Query: 19  EDSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVSVRINGDGPRNSSGSTTPP 78
           ED       P+SP       +   S    KK RR ++K+VVSV I       S G   PP
Sbjct: 14  EDETYNDVVPESPSSCEDSKI---SKPTPKKSRRNVEKRVVSVPIADVEGSKSRGEVYPP 73

Query: 79  SDSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDPTMLLITYSCEHNHSG 138
           SDSWAWRKYGQKPIKGSPYPR YYRCSSSKGCPARKQVER+R+DP+ L+ITY+C+HNH  
Sbjct: 74  SDSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLMITYACDHNHPF 133

Query: 139 PVSRNN--NHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLEDQFSWFGEMETTSSTVL 198
           P S  N  +HH+++ V  T+ +      E E E EE  V + E+  +   ++    S +L
Sbjct: 134 PSSSANTKSHHRSSVVLKTAKK----EEEYEEEEEELTVTAAEEPPAGL-DLSHVDSPLL 193

Query: 199 ESSIFSG------------RSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELPECSV 258
               +S              SSSG  + +   +   F +G ++ D+SLF DLG+LP+C+ 
Sbjct: 194 LGGCYSEIGEFGWFYDASISSSSGSSNFLDVTLERGFSVGQEE-DESLFGDLGDLPDCAS 253

Query: 259 VFRHGGGPGLPVDEQPATSQRRITPWCGAT 275
           VFR G    +  +EQ         P+C ++
Sbjct: 254 VFRRG---TVATEEQHRRCDFGAIPFCDSS 271

BLAST of CmaCh13G005850 vs. TAIR 10
Match: AT3G58710.2 (WRKY DNA-binding protein 69 )

HSP 1 Score: 159.8 bits (403), Expect = 3.0e-39
Identity = 109/269 (40.52%), Postives = 149/269 (55.39%), Query Frame = 0

Query: 20  DSDEFKAAPDSPPPPPPPPLPPNSTAAAKKGRRGMKKKVVSVRINGDGPRNSSGSTTPPS 79
           D +E +   D  P  P        +    K RR ++K+VVSV I       S G   PPS
Sbjct: 11  DDEEDETYNDVVPESPSSCEDSKISKPTPKKRRNVEKRVVSVPIADVEGSKSRGEVYPPS 70

Query: 80  DSWAWRKYGQKPIKGSPYPRAYYRCSSSKGCPARKQVERNRLDPTMLLITYSCEHNHSGP 139
           DSWAWRKYGQKPIKGSPYPR YYRCSSSKGCPARKQVER+R+DP+ L+ITY+C+HNH  P
Sbjct: 71  DSWAWRKYGQKPIKGSPYPRGYYRCSSSKGCPARKQVERSRVDPSKLMITYACDHNHPFP 130

Query: 140 VSRNN--NHHQAAAVKPTSPETVPVHPEPEPEAEEKFVESLEDQFSWFGEMETTSSTVLE 199
            S  N  +HH+++ V  T+ +      E E E EE  V + E+  +   ++    S +L 
Sbjct: 131 SSSANTKSHHRSSVVLKTAKK----EEEYEEEEEELTVTAAEEPPAGL-DLSHVDSPLLL 190

Query: 200 SSIFSG------------RSSSGVGDSISSDVAMPFPMGDDDVDDSLFADLGELPECSVV 259
              +S              SSSG  + +   +   F +G ++ D+SLF DLG+LP+C+ V
Sbjct: 191 GGCYSEIGEFGWFYDASISSSSGSSNFLDVTLERGFSVGQEE-DESLFGDLGDLPDCASV 250

Query: 260 FRHGGGPGLPVDEQPATSQRRITPWCGAT 275
           FR G    +  +EQ         P+C ++
Sbjct: 251 FRRG---TVATEEQHRRCDFGAIPFCDSS 270

BLAST of CmaCh13G005850 vs. TAIR 10
Match: AT1G30650.1 (WRKY DNA-binding protein 14 )

HSP 1 Score: 125.6 bits (314), Expect = 6.3e-29
Identity = 92/216 (42.59%), Postives = 109/216 (50.46%), Query Frame = 0

Query: 49  KGRRGMKKKVVSVRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSK 108
           K R+   KKVV +         SSG    PSD WAWRKYGQKPIKGSP+PR YYRCSSSK
Sbjct: 188 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPFPRGYYRCSSSK 247

Query: 109 GCPARKQVERNRLDPTMLLITYSCEHNHSGPVSRNNNHHQAAAVKPTSPETVPVHPEP-E 168
           GC ARKQVER+R DP ML+ITY+ EHNH  P+ RN     A A    S  +   +P P +
Sbjct: 248 GCSARKQVERSRTDPNMLVITYTSEHNHPWPIQRN-----ALAGSTRSSTSSSSNPNPSK 307

Query: 169 PEAEEKFVESLEDQFSWFGEMETTSSTVLESSIFSGRSS-----SGVGDSISSDVAMPFP 228
           P        S+  Q + +    TT    L SS              V D   + +A   P
Sbjct: 308 PSTANVNSSSIGSQNTIYLPSSTTPPPTLSSSAIKDERGDDMELENVDDDDDNQIAPYRP 367

Query: 229 MGDD--DVDDSLFADLGEL--PECSVVFRHG-GGPG 254
              D     D  FADL EL     S++  HG GG G
Sbjct: 368 ELHDHQHQPDDFFADLEELEGDSLSMLLSHGCGGDG 397

BLAST of CmaCh13G005850 vs. TAIR 10
Match: AT2G34830.1 (WRKY DNA-binding protein 35 )

HSP 1 Score: 125.6 bits (314), Expect = 6.3e-29
Identity = 62/95 (65.26%), Postives = 67/95 (70.53%), Query Frame = 0

Query: 49  KGRRGMKKKVVSVRINGDGPRNSSGSTTPPSDSWAWRKYGQKPIKGSPYPRAYYRCSSSK 108
           K R+   KKVV +         SSG    PSD WAWRKYGQKPIKGSPYPR YYRCSSSK
Sbjct: 186 KRRKSQAKKVVCIPAPAAMNSRSSGEVV-PSDLWAWRKYGQKPIKGSPYPRGYYRCSSSK 245

Query: 109 GCPARKQVERNRLDPTMLLITYSCEHNHSGPVSRN 144
           GC ARKQVER+R DP ML+ITY+ EHNH  P  RN
Sbjct: 246 GCSARKQVERSRTDPNMLVITYTSEHNHPWPTQRN 279

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LP561.3e-5556.88Probable WRKY transcription factor 65 OS=Arabidopsis thaliana OX=3702 GN=WRKY65 ... [more]
Q93WV54.3e-3840.52Probable WRKY transcription factor 69 OS=Arabidopsis thaliana OX=3702 GN=WRKY69 ... [more]
Q9SA808.9e-2842.59Probable WRKY transcription factor 14 OS=Arabidopsis thaliana OX=3702 GN=WRKY14 ... [more]
O647478.9e-2865.26Probable WRKY transcription factor 35 OS=Arabidopsis thaliana OX=3702 GN=WRKY35 ... [more]
O046091.1e-2539.90WRKY transcription factor 22 OS=Arabidopsis thaliana OX=3702 GN=WRKY22 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KJW53.4e-155100.00probable WRKY transcription factor 65 isoform X1 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1KF848.7e-15198.55probable WRKY transcription factor 65 isoform X2 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1EGX59.0e-14898.18probable WRKY transcription factor 65 isoform X1 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1EGD72.3e-14396.73probable WRKY transcription factor 65 isoform X2 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1EK891.6e-13693.45probable WRKY transcription factor 65 isoform X3 OS=Cucurbita moschata OX=3662 G... [more]
Match NameE-valueIdentityDescription
XP_023001000.17.0e-155100.00probable WRKY transcription factor 65 isoform X1 [Cucurbita maxima][more]
KAG6583943.13.3e-15298.20putative WRKY transcription factor 65, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023001002.11.8e-15098.55probable WRKY transcription factor 65 isoform X2 [Cucurbita maxima][more]
KAG7019560.14.0e-15098.19putative WRKY transcription factor 65, partial [Cucurbita argyrosperma subsp. ar... [more]
XP_022927161.11.9e-14798.18probable WRKY transcription factor 65 isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G29280.19.4e-5756.88WRKY DNA-binding protein 65 [more]
AT3G58710.13.6e-4041.11WRKY DNA-binding protein 69 [more]
AT3G58710.23.0e-3940.52WRKY DNA-binding protein 69 [more]
AT1G30650.16.3e-2942.59WRKY DNA-binding protein 14 [more]
AT2G34830.16.3e-2965.26WRKY DNA-binding protein 35 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 78..138
e-value: 2.4E-34
score: 130.1
IPR003657WRKY domainPFAMPF03106WRKYcoord: 80..136
e-value: 7.8E-25
score: 86.9
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 79..139
score: 31.203669
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 68..140
e-value: 1.1E-29
score: 104.0
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 75..138
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 138..171
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 250..275
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 17..86
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 27..43
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 65..82
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 138..152
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 261..275
NoneNo IPR availablePANTHERPTHR32096:SF19OS01G0750100 PROTEINcoord: 42..273
IPR044810WRKY transcription factor, plantPANTHERPTHR32096WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATEDcoord: 42..273

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh13G005850.1CmaCh13G005850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0000976 transcription cis-regulatory region binding
molecular_function GO:0043565 sequence-specific DNA binding