CmaCh13G005630 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh13G005630
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	PLASMODESMATA CALLOSE-BINDING PROTEIN 1-like
Location	Cma_Chr13: 5723854 .. 5725027 (+)
RNA-Seq Expression	CmaCh13G005630
Synteny	CmaCh13G005630

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTATCAACATTCTGAGAAATTTGGCACTATTTGCTATCTATACCTTCTTGTTCTTCTCAGGTAATGATGTTTTTACCATTCTTTGTAGCTATTTCTATGCTCATTGTTACCTAAGTTTGAAAGTGAAAGTAAGAGAATAAGTTTTGATGTAGATATGACAATGTCTGCAAACAGAATGATATTTTTTTTAATGGGAAACAAAGGATAACATTCAAAACTGCACTAAGTCCTAACAAAATAACTTGTGTTGTCATTGTCAATTCTACTGCATATTGAATTTTTGATGCAATTCAAATCCACTACATGCTATCCAGAAAAGTAATTTAGTCATAATTCAGTGATTGCAGCAAATTTGACATAAAGAAGGTGAAAATTTGATGCAGTTTTGATCTCGTCAGGTTCAAGTTTAGCATGGAAATCACGTATACAAGAAGAACATCAAGAAAACATACCTTTGAAGAATGAAGAAGACAGAACAATCTTCTCTCCTCTACATTTCACTCAGTTGGTTGACTCTACCATTGTTAATCCAACAACAACGCCAGGACAGTCTGTACCAAACTCAGTAGAACCCAATCTGAAACCCACAACAGGGCCAGGGAGATCTGGTGGAGGTTCATGGTGTATTGCAAGCTCTGCAGCTTCTCCAACAGCTCTGCAGGTGGCTCTTGACTATGCCTGTGGCTATGGCAGTACAGACTGTTCGGCGATTCAGCCGGGTGGTAGCTCCTATGAGCTGACACAGTGAAGGACCATGCTTCTTATGCCTTCGATGACTATTACCAAAGGAATCCAGTGGCTACTAGCTGTGATTTTGGTGGAACAGCACAACTTGTTAGTACAAACCCAAGTATGGCCTTTTCACCTCTCTGACTCATAGTTAACCCCTGTTGTCCCTTGTGGTACACAATTTTAGCTAAAACAGGTCTATTGATTACAGGAGTTTCTACTTAGTCTACTTAACTAGCAAATTTTTTTAGTTAACAATTGCATTTTCCATATTATATTGCAGGTTATGGTAACTGTCACTATGCAAAACCCATAGATCTATCAATTTATGGTGCAAGACCATCAGGCATGCCCAGCTCAGTCATTTCAATATCAAAACGATCAGTGCTACTCTTGACCATGACTCACCTTTTGTGTTCGCTCTTGGAAAATCATCTGTAA

mRNA sequence

ATGGGTATCAACATTCTGAGAAATTTGGCACTATTTGCTATCTATACCTTCTTGTTCTTCTCAGGTTCAAGTTTAGCATGGAAATCACGTATACAAGAAGAACATCAAGAAAACATACCTTTGAAGAATGAAGAAGACAGAACAATCTTCTCTCCTCTACATTTCACTCAGTTGGTTGACTCTACCATTGTTAATCCAACAACAACGCCAGGACAGTCTGTACCAAACTCAGTAGAACCCAATCTGAAACCCACAACAGGGCCAGGGAGATCTGGTGGAGGTTCATGGTGTATTGCAAGCTCTGCAGCTTCTCCAACAGCTCTGCAGGTGGCTCTTGACTATGCCTGTGGCTATGGCAGTACAGACTGTTCGGCGATTCAGCCGGGTGTGAAGGACCATGCTTCTTATGCCTTCGATGACTATTACCAAAGGAATCCAGTGGCTACTAGCTGTGATTTTGGTGGAACAGCACAACTTGTTAGTACAAACCCAAGTTATGGTAACTGTCACTATGCAAAACCCATAGATCTATCAATTTATGGTGCAAGACCATCAGGCATGCCCAGCTCAGTCATTTCAATATCAAAACGATCAGTGCTACTCTTGACCATGACTCACCTTTTGTGTTCGCTCTTGGAAAATCATCTGTAA

Coding sequence (CDS)

ATGGGTATCAACATTCTGAGAAATTTGGCACTATTTGCTATCTATACCTTCTTGTTCTTCTCAGGTTCAAGTTTAGCATGGAAATCACGTATACAAGAAGAACATCAAGAAAACATACCTTTGAAGAATGAAGAAGACAGAACAATCTTCTCTCCTCTACATTTCACTCAGTTGGTTGACTCTACCATTGTTAATCCAACAACAACGCCAGGACAGTCTGTACCAAACTCAGTAGAACCCAATCTGAAACCCACAACAGGGCCAGGGAGATCTGGTGGAGGTTCATGGTGTATTGCAAGCTCTGCAGCTTCTCCAACAGCTCTGCAGGTGGCTCTTGACTATGCCTGTGGCTATGGCAGTACAGACTGTTCGGCGATTCAGCCGGGTGTGAAGGACCATGCTTCTTATGCCTTCGATGACTATTACCAAAGGAATCCAGTGGCTACTAGCTGTGATTTTGGTGGAACAGCACAACTTGTTAGTACAAACCCAAGTTATGGTAACTGTCACTATGCAAAACCCATAGATCTATCAATTTATGGTGCAAGACCATCAGGCATGCCCAGCTCAGTCATTTCAATATCAAAACGATCAGTGCTACTCTTGACCATGACTCACCTTTTGTGTTCGCTCTTGGAAAATCATCTGTAA

Protein sequence

MGINILRNLALFAIYTFLFFSGSSLAWKSRIQEEHQENIPLKNEEDRTIFSPLHFTQLVDSTIVNPTTTPGQSVPNSVEPNLKPTTGPGRSGGGSWCIASSAASPTALQVALDYACGYGSTDCSAIQPGVKDHASYAFDDYYQRNPVATSCDFGGTAQLVSTNPSYGNCHYAKPIDLSIYGARPSGMPSSVISISKRSVLLLTMTHLLCSLLENHL

Homology

[Close]

BLAST of CmaCh13G005630 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 5.0e-18
Identity = 58/127 (45.67%), Postives = 73/127 (57.48%), Query Frame = 0


Query: 60  DSTIVNPT-----TTPGQSVPNSVEPNLKPTTGPGRSGG-GSWCIASSAASPTALQVALD 119
           ++T V+PT     T+P  S    +  N   T G G  GG   WCIASS AS T LQ ALD
Sbjct: 349 NTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALD 408

Query: 120 YACGYGSTDCSAIQPG--------VKDHASYAFDDYYQRNPVAT-SCDFGGTAQLVSTNP 172
           +ACG G+ DCSA+QP         V  HASYAF+ YYQ++  ++  C F G +  V  +P
Sbjct: 409 WACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDP 468

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BLAST of CmaCh13G005630 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 6.8e-15
Identity = 51/132 (38.64%), Postives = 70/132 (53.03%), Query Frame = 0


Query: 50  FSPLHFTQLV-DSTIVNPTTTPGQSVPNSVEPNLKPTTGPGRSGGGSWCIASSAASPTAL 109
           F+P++   L+ D   V PT +P         P+  P+  P  SGGG WC+A   A+ T L
Sbjct: 338 FTPVYDLGLLRDGASVAPTPSP--------NPSPNPSPKPAPSGGGKWCVAKDGANGTDL 397

Query: 110 QVALDYACGYGSTDCSAIQPG--------VKDHASYAFDDYYQRN-PVATSCDFGGTAQL 169
           Q  ++YACG+   DC  IQ G        ++ HASY  + YYQ N     +CDF GT  +
Sbjct: 398 QNNINYACGF--VDCKPIQSGGACFSPNSLQAHASYVMNAYYQANGHTDLACDFKGTGIV 457

Query: 170 VSTNPSYGNCHY 172
            S++PSYG C Y
Sbjct: 458 TSSDPSYGGCKY 459

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BLAST of CmaCh13G005630 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 79.3 bits (194), Expect = 5.8e-14
Identity = 40/84 (47.62%), Postives = 54/84 (64.29%), Query Frame = 0


Query: 95  SWCIASSAASPTALQVALDYACGYGSTDCSAIQPG--------VKDHASYAFDDYYQRNP 154
           ++CIA        LQ ALD+ACG G ++CS IQPG        VK HAS+AF+ YYQ+  
Sbjct: 380 TYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQKEG 439

Query: 155 VAT-SCDFGGTAQLVSTNPSYGNC 170
            A+ SCDF G A + +T+PS+G+C
Sbjct: 440 RASGSCDFKGVAMITTTDPSHGSC 463

[Close]

BLAST of CmaCh13G005630 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.9e-13
Identity = 50/121 (41.32%), Postives = 63/121 (52.07%), Query Frame = 0


Query: 60  DSTIVNPTTTPGQSVPNSVEPNLKPTTGPGRSGGGSWCIASSAASPTALQVALDYACGYG 119
           D T V      G+S  N    N   T   G S   SWCIASS AS   L+ ALD+ACG G
Sbjct: 337 DQTSVYQLDFTGKS--NGFHSNSSGTNSSGSS--NSWCIASSKASERDLKGALDWACGPG 396

Query: 120 STDCSAIQPG--------VKDHASYAFDDYYQRNPVA-TSCDFGGTAQLVSTNPSYGNCH 172
           + DC+AIQP         +  HAS+ F+ Y+Q+N     +C FGG    V+ +PSY  C 
Sbjct: 397 NVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQQNRATDVACSFGGAGVKVNKDPSYDKCI 453

[Close]

BLAST of CmaCh13G005630 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 4.1e-12
Identity = 38/90 (42.22%), Postives = 52/90 (57.78%), Query Frame = 0


Query: 91  SGGGSWCIASSAASPTALQVALDYACGYGSTDCSAIQPG--------VKDHASYAFDDYY 150
           +G   +C+A + A    L   L++ACG G  +C+AIQPG        VK HAS+AF+DYY
Sbjct: 357 NGSSMFCVAKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYY 416

Query: 151 QR-NPVATSCDFGGTAQLVSTNPSYGNCHY 172
           Q+      +CDF GTA   + +PSY  C Y
Sbjct: 417 QKMKSAGGTCDFDGTAITTTRDPSYRTCAY 446

[Close]

BLAST of CmaCh13G005630 vs. ExPASy TrEMBL
Match: A0A6J1ELB1 (major pollen allergen Ole e 10-like OS=Cucurbita moschata OX=3662 GN=LOC111434346 PE=4 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 3.7e-80
Identity = 156/173 (90.17%), Postives = 159/173 (91.91%), Query Frame = 0


Query: 1   MGINILRNLALFAIYTFLFFSGSSLAWKSRIQEEHQENIPLKNEEDRTIFSPLHFTQLVD 60
           MGINILRNLALFAIYTFLFFSGSSLAWKSRIQEEHQEN+PLKNEEDRTIFSPLHFTQLVD
Sbjct: 1   MGINILRNLALFAIYTFLFFSGSSLAWKSRIQEEHQENVPLKNEEDRTIFSPLHFTQLVD 60

Query: 61  STIVNPTTTPGQSVPNSVEPNLKPTTGPGRSGGGSWCIASSAASPTALQVALDYACGYGS 120
           STIVNPTTTPGQSVPNSV+ NLKPTTGPGRS G SWC ASSAASPTALQVALDYACGY  
Sbjct: 61  STIVNPTTTPGQSVPNSVKSNLKPTTGPGRSSGVSWCTASSAASPTALQVALDYACGYSG 120

Query: 121 TDCSAIQPG--------VKDHASYAFDDYYQRNPVATSCDFGGTAQLVSTNPS 166
           TDCSAIQPG        VKDHASYAF+DYYQRNPVATSCDFGGTAQLVSTNPS
Sbjct: 121 TDCSAIQPGGSCYEPNTVKDHASYAFNDYYQRNPVATSCDFGGTAQLVSTNPS 173

[Close]

BLAST of CmaCh13G005630 vs. ExPASy TrEMBL
Match: A0A1S3B947 (PLASMODESMATA CALLOSE-BINDING PROTEIN 4-like OS=Cucumis melo OX=3656 GN=LOC103487389 PE=4 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 7.2e-76
Identity = 169/281 (60.14%), Postives = 186/281 (66.19%), Query Frame = 0


Query: 1   MGINILRNLALFAIYTFLFFSGSSLAWKSRIQEEHQENIPLKNEEDRTIFSPLHFTQLVD 60
           MG NILR+LALFA+Y+FL FSGSSLA KS IQEE+QENIPL+NEEDRTI SP+HFTQL D
Sbjct: 1   MGFNILRDLALFALYSFLLFSGSSLAEKSSIQEENQENIPLQNEEDRTIVSPIHFTQLDD 60

Query: 61  STIVNPTT------TPGQSVPNSVEPNLKPTTGPGRSGGGSWCIASSAASPTALQVALDY 120
           +TIVNPTT       P QSVPN V+P++ PT   G  GGGSWCIASSAASPTALQVALDY
Sbjct: 61  TTIVNPTTPGGTPVAPPQSVPNIVDPSVNPTAVSGNPGGGSWCIASSAASPTALQVALDY 120

Query: 121 ACGYGSTDCSAIQPG--------VKDHASYAFDDYYQRNPVATSCDFGGTAQLVSTNPSY 180
           ACGYG  DCSAIQPG        VKDHASYAF+DYYQ+NP ATSC FGGTAQLVST+PS 
Sbjct: 121 ACGYGGADCSAIQPGGSCYDPNTVKDHASYAFNDYYQKNPAATSCVFGGTAQLVSTDPSN 180

Query: 181 GNCHYAK---------------------------------------------------PI 217
           GNCHYAK                                                   P 
Sbjct: 181 GNCHYAKPGAVLSPPPPATPPPPAPVIPTPTPPAPTVPTPPPPATTNPTYTPTNPTYTPT 240

[Close]

BLAST of CmaCh13G005630 vs. ExPASy TrEMBL
Match: A0A0A0M054 (X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G459490 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 1.1e-73
Identity = 168/281 (59.79%), Postives = 185/281 (65.84%), Query Frame = 0


Query: 1   MGINILRNLALFAIYTFLFFSGSSLAWKSRIQEEHQENIPLKNEEDRTIFSP-LHFTQLV 60
           MG NILR+LALFA+ +FL FSGSSLA KS IQEE+QENIPL+NEEDRTI SP LHFTQL 
Sbjct: 1   MGFNILRDLALFALCSFLLFSGSSLAEKSPIQEENQENIPLQNEEDRTIVSPSLHFTQLD 60

Query: 61  DSTIVNPTTT------PGQSVPNSVEPNLKPTTGPGRSGGGSWCIASSAASPTALQVALD 120
           D+TIVNPTT+      P QSVPN V+PN+ PT   G  GGGSWCIA+SAASPTALQVALD
Sbjct: 61  DTTIVNPTTSGGTPVAPPQSVPNIVDPNVNPTAVSGNPGGGSWCIANSAASPTALQVALD 120

Query: 121 YACGYGSTDCSAIQPG--------VKDHASYAFDDYYQRNPVATSCDFGGTAQLVSTNPS 180
           YACGYG  DCSAIQPG        VKDHASYAF+DYYQ+NP ATSC FGGTAQLVST+PS
Sbjct: 121 YACGYGGADCSAIQPGGSCYDPNTVKDHASYAFNDYYQKNPAATSCVFGGTAQLVSTDPS 180

Query: 181 YGNCHYAK--------------------------------------------------PI 217
            GNCHYAK                                                  P 
Sbjct: 181 NGNCHYAKPGAVLSPPPATPPPPAPVIPTPTPPAPTVETPPPPATTNPTYTPTNPTYTPT 240

[Close]

BLAST of CmaCh13G005630 vs. ExPASy TrEMBL
Match: A0A6J1JX26 (PLASMODESMATA CALLOSE-BINDING PROTEIN 1-like OS=Cucurbita maxima OX=3661 GN=LOC111490458 PE=4 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 4.8e-72
Identity = 168/285 (58.95%), Postives = 185/285 (64.91%), Query Frame = 0


Query: 1   MGINILRNLALFAIYTFLFFSGSSLAWKSRIQEEHQENIPLKNEEDRTIFSP-LHFTQLV 60
           MG NILRNLAL     FL FSGSS A K  IQEE+Q++IPL++EEDR IFSP +HFTQL 
Sbjct: 1   MGSNILRNLALL----FLLFSGSSFAEKPPIQEENQQDIPLQSEEDRKIFSPSVHFTQLD 60

Query: 61  DSTIVNPTTTPG-------------QSVPNSVEPN-LKPTTGPGRSGGGSWCIASSAASP 120
           D+ IVNP TTPG             Q+VPNSV+PN + PTTGPG SGGGSWCIASSAASP
Sbjct: 61  DAPIVNPATTPGEVQTPTVNPTTSPQTVPNSVDPNMMTPTTGPGNSGGGSWCIASSAASP 120

Query: 121 TALQVALDYACGYGSTDCSAIQPG--------VKDHASYAFDDYYQRNPVATSCDFGGTA 180
           TALQVALDYACGYG  DCSAIQPG        VKDHASYAF+DYYQ+NP ATSC FGGTA
Sbjct: 121 TALQVALDYACGYGGADCSAIQPGGSCYDPNTVKDHASYAFNDYYQKNPAATSCVFGGTA 180

Query: 181 QLVSTNPSYGNCHYA--------------------------------------------- 217
           QLVST+PS GNCHYA                                             
Sbjct: 181 QLVSTDPSNGNCHYATSRAVPSPPPPAVPNPTPPPPVIPTPTPPITTIPTMTPPAPTNPT 240

[Close]

BLAST of CmaCh13G005630 vs. ExPASy TrEMBL
Match: A0A6J1GUD6 (PLASMODESMATA CALLOSE-BINDING PROTEIN 1-like OS=Cucurbita moschata OX=3662 GN=LOC111457203 PE=4 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.8e-71
Identity = 168/285 (58.95%), Postives = 184/285 (64.56%), Query Frame = 0


Query: 1   MGINILRNLALFAIYTFLFFSGSSLAWKSRIQEEHQENIPLKNEEDRTIFSP-LHFTQLV 60
           MG NILRNLALF    FL FSGSS A K  IQEE+Q++IPL++EEDR IFSP +HFTQL 
Sbjct: 1   MGSNILRNLALF----FLLFSGSSFAEKPPIQEENQQDIPLQSEEDRKIFSPSVHFTQLD 60

Query: 61  DSTIVNPTTTPG-------------QSVPNSVEPN-LKPTTGPGRSGGGSWCIASSAASP 120
           D  IVNP TTPG             Q+VPNSV PN + PTTGPG SGGGSWCIASSAASP
Sbjct: 61  DVPIVNPATTPGEVQTPIVNPTTSPQTVPNSVNPNMMTPTTGPGNSGGGSWCIASSAASP 120

Query: 121 TALQVALDYACGYGSTDCSAIQPG--------VKDHASYAFDDYYQRNPVATSCDFGGTA 180
           TALQVALDYACGYG  DCSAIQPG        VKDHASYAF+DYYQRNP ATSC FGGTA
Sbjct: 121 TALQVALDYACGYGGADCSAIQPGGSCYDPNTVKDHASYAFNDYYQRNPAATSCVFGGTA 180

Query: 181 QLVSTNPSYGNCHYA--------------------------------------------- 217
           QLVST+PS GNCH+A                                             
Sbjct: 181 QLVSTDPSNGNCHFATSRAVPSPPPPAVPNPTPPPPVIPTPTPPITTIPTMTPPAPTNPT 240

[Close]

BLAST of CmaCh13G005630 vs. NCBI nr
Match: KAG7019539.1 (Glucan endo-1,3-beta-glucosidase 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 362.5 bits (929), Expect = 2.6e-96
Identity = 184/202 (91.09%), Postives = 186/202 (92.08%), Query Frame = 0


Query: 23  SSLAWKSRIQEEHQENIPLKNEEDRTIFSPLHFTQLVDSTIVNPTTTPGQSVPNSVEPNL 82
           SSLAWKSRIQEEHQEN+PLKNEEDRTIFSPLHFTQLVDSTIVNPTTTPGQSVPNSVE NL
Sbjct: 1   SSLAWKSRIQEEHQENVPLKNEEDRTIFSPLHFTQLVDSTIVNPTTTPGQSVPNSVESNL 60

Query: 83  KPTTGPGRSGGGSWCIASSAASPTALQVALDYACGYGSTDCSAIQPG--------VKDHA 142
           KPTTGPGRS G SWC ASSAASPTALQVALDYACGY  TDCSAIQPG        VKDHA
Sbjct: 61  KPTTGPGRSSGVSWCTASSAASPTALQVALDYACGYSGTDCSAIQPGGSCYEPNTVKDHA 120

Query: 143 SYAFDDYYQRNPVATSCDFGGTAQLVSTNPSYGNCHYAKPIDLSIYGARPSGMPSSVISI 202
           SYAF+DYYQRNPVATSCDFGGTAQLVSTNPSYGNCHYAKPIDLSIYGARPSGMPSSVISI
Sbjct: 121 SYAFNDYYQRNPVATSCDFGGTAQLVSTNPSYGNCHYAKPIDLSIYGARPSGMPSSVISI 180

Query: 203 SKRSVLLLTMTHLLCSLLENHL 217
           SKRSVLLLTMTHLLCS L NHL
Sbjct: 181 SKRSVLLLTMTHLLCSHLANHL 202

[Close]

BLAST of CmaCh13G005630 vs. NCBI nr
Match: XP_022927548.1 (major pollen allergen Ole e 10-like [Cucurbita moschata])

HSP 1 Score: 307.8 bits (787), Expect = 7.6e-80
Identity = 156/173 (90.17%), Postives = 159/173 (91.91%), Query Frame = 0


Query: 1   MGINILRNLALFAIYTFLFFSGSSLAWKSRIQEEHQENIPLKNEEDRTIFSPLHFTQLVD 60
           MGINILRNLALFAIYTFLFFSGSSLAWKSRIQEEHQEN+PLKNEEDRTIFSPLHFTQLVD
Sbjct: 1   MGINILRNLALFAIYTFLFFSGSSLAWKSRIQEEHQENVPLKNEEDRTIFSPLHFTQLVD 60

Query: 61  STIVNPTTTPGQSVPNSVEPNLKPTTGPGRSGGGSWCIASSAASPTALQVALDYACGYGS 120
           STIVNPTTTPGQSVPNSV+ NLKPTTGPGRS G SWC ASSAASPTALQVALDYACGY  
Sbjct: 61  STIVNPTTTPGQSVPNSVKSNLKPTTGPGRSSGVSWCTASSAASPTALQVALDYACGYSG 120

Query: 121 TDCSAIQPG--------VKDHASYAFDDYYQRNPVATSCDFGGTAQLVSTNPS 166
           TDCSAIQPG        VKDHASYAF+DYYQRNPVATSCDFGGTAQLVSTNPS
Sbjct: 121 TDCSAIQPGGSCYEPNTVKDHASYAFNDYYQRNPVATSCDFGGTAQLVSTNPS 173

[Close]

BLAST of CmaCh13G005630 vs. NCBI nr
Match: KAG6583926.1 (Pre-mRNA-processing factor 39, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 306.2 bits (783), Expect = 2.2e-79
Identity = 155/172 (90.12%), Postives = 158/172 (91.86%), Query Frame = 0


Query: 1   MGINILRNLALFAIYTFLFFSGSSLAWKSRIQEEHQENIPLKNEEDRTIFSPLHFTQLVD 60
           MGINILRNLALFAIYTFLFFSGSSLAWKSRIQEEHQEN+PLKNEEDRTIFSPLHFTQLVD
Sbjct: 1   MGINILRNLALFAIYTFLFFSGSSLAWKSRIQEEHQENVPLKNEEDRTIFSPLHFTQLVD 60

Query: 61  STIVNPTTTPGQSVPNSVEPNLKPTTGPGRSGGGSWCIASSAASPTALQVALDYACGYGS 120
           STIVNPTTTPGQSVPNSV+ NLKPTTGPGRS G SWC ASSAASPTALQVALDYACGY  
Sbjct: 61  STIVNPTTTPGQSVPNSVKSNLKPTTGPGRSSGVSWCTASSAASPTALQVALDYACGYSG 120

Query: 121 TDCSAIQPG--------VKDHASYAFDDYYQRNPVATSCDFGGTAQLVSTNP 165
           TDCSAIQPG        VKDHASYAF+DYYQRNPVATSCDFGGTAQLVSTNP
Sbjct: 121 TDCSAIQPGGSCYEPNTVKDHASYAFNDYYQRNPVATSCDFGGTAQLVSTNP 172

[Close]

BLAST of CmaCh13G005630 vs. NCBI nr
Match: XP_023519757.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 298.1 bits (762), Expect = 6.0e-77
Identity = 155/173 (89.60%), Postives = 157/173 (90.75%), Query Frame = 0


Query: 1   MGINILRNLALFAIYTFLFFSGSSLAWKSRIQEEHQENIPLKNEEDRTIFSPLHFTQLVD 60
           MGINILRNL L AIYTFLF SGSSLAWKSRIQ EHQENIPLKNEEDRTIFSPL+FTQLVD
Sbjct: 1   MGINILRNLTLLAIYTFLFLSGSSLAWKSRIQ-EHQENIPLKNEEDRTIFSPLYFTQLVD 60

Query: 61  STIVNPTTTPGQSVPNSVEPNLKPTTGPGRSGGGSWCIASSAASPTALQVALDYACGYGS 120
           STIVNPTTTP QSVPNSVE NLKPTTGPGRS G SWCIASSAASPTALQVALDYACGYGS
Sbjct: 61  STIVNPTTTPAQSVPNSVESNLKPTTGPGRSSGVSWCIASSAASPTALQVALDYACGYGS 120

Query: 121 TDCSAIQPG--------VKDHASYAFDDYYQRNPVATSCDFGGTAQLVSTNPS 166
           TDCSAIQPG        VKDHASYAF+DYYQRNPVATSCDFGGTAQLVSTNPS
Sbjct: 121 TDCSAIQPGGSCYEPNTVKDHASYAFNDYYQRNPVATSCDFGGTAQLVSTNPS 172

[Close]

BLAST of CmaCh13G005630 vs. NCBI nr
Match: XP_038896033.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 4-like [Benincasa hispida])

HSP 1 Score: 295.4 bits (755), Expect = 3.9e-76
Identity = 170/278 (61.15%), Postives = 187/278 (67.27%), Query Frame = 0


Query: 1   MGINILRNLALFAIYTFLFFSGSSLAWKSRIQEEHQENIPLKNEEDRTIFSP-LHFTQLV 60
           MG+NILRNLALF + +FL FSGSS A KS IQE +++NIPL+NE+DRTIFSP +HFTQL 
Sbjct: 1   MGLNILRNLALFTLCSFLLFSGSSFAEKSPIQEANKKNIPLQNEDDRTIFSPSVHFTQLD 60

Query: 61  DSTIVNPTTTPG-------QSVPNSVEPNLKPTTGPGRSGGGSWCIASSAASPTALQVAL 120
           D+TIVNPTTTPG       QSVPN V+PN+ PT   G SGGGSWCIASSAASPTALQVAL
Sbjct: 61  DTTIVNPTTTPGGTPVSPPQSVPNLVDPNVNPTAVTGNSGGGSWCIASSAASPTALQVAL 120

Query: 121 DYACGYGSTDCSAIQPG--------VKDHASYAFDDYYQRNPVATSCDFGGTAQLVSTNP 180
           DYACGYG  DCSAIQPG        VKDHASYAF+DYYQ+NP ATSC FGGTAQLVST+P
Sbjct: 121 DYACGYGGADCSAIQPGGSCYDPNTVKDHASYAFNDYYQKNPAATSCVFGGTAQLVSTDP 180

Query: 181 SYGNCHYA----------------------------------------------KPIDLS 217
           S GNCHYA                                               P D S
Sbjct: 181 SNGNCHYATPRAMPSPPPPANPNPTPPAPVIPPPPPPATTIPTMTPPDTTNPTYTPTDPS 240

[Close]

BLAST of CmaCh13G005630 vs. TAIR 10
Match: AT1G29380.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 115.9 bits (289), Expect = 4.0e-26
Identity = 72/179 (40.22%), Postives = 92/179 (51.40%), Query Frame = 0


Query: 85  TTGPGRSGGGSWCIASSAASPTALQVALDYACGYGSTDCSAIQPG--------VKDHASY 144
           T   G  G G WCIA + ASPT+LQVALDYACGYG  DC  IQ G        ++DHAS+
Sbjct: 137 TGAGGGVGSGQWCIAKANASPTSLQVALDYACGYGGADCGQIQQGAACYEPNTIRDHASF 196

Query: 145 AFDDYYQRNPVATSCDFGGTAQLVSTNPSYGNCH-------------------------- 204
           AF+ YYQ++P + SC+FGG AQL ST+PS G+CH                          
Sbjct: 197 AFNSYYQKHPGSDSCNFGGAAQLTSTDPSKGSCHFSSSSGTVSTSPPSQMSPPDFSSPPS 256

Query: 205 --YAKPIDLSIYG----------ARPSGMPSSVISISKRSVLLLTMTHLLCSLL-ENHL 217
             Y  PI     G          A P+G+P+S  S+S   + + T   +L  LL EN+L
Sbjct: 257 STYPPPITTPTTGITGSGPPFGVAEPTGLPNSATSVSHSLLSIFTAVGILMPLLRENYL 315

[Close]

BLAST of CmaCh13G005630 vs. TAIR 10
Match: AT2G30933.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 104.4 bits (259), Expect = 1.2e-22
Identity = 70/196 (35.71%), Postives = 96/196 (48.98%), Query Frame = 0


Query: 44  EEDRTIFSPLHFTQLVDSTIVNPTTTPGQSVPNS--------VEPNLKPTT--GPGRSGG 103
           EE++ I +PL           NPTTTP   VPNS          P   P++  G    G 
Sbjct: 29  EEEKDITTPL---------ATNPTTTPTTVVPNSDSDASAVATTPLTIPSSPHGVAYPGD 88

Query: 104 GSWCIASSAASPTALQVALDYACGYGSTDCSAIQPG--------VKDHASYAFDDYYQRN 163
            SWC+A    +  ALQ ALDYACG G  DCS IQ G        ++ HAS+AF+ YYQ+N
Sbjct: 89  QSWCVARENVAKMALQAALDYACGIGGADCSEIQEGGNCYNPNSLRAHASFAFNSYYQKN 148

Query: 164 PVATSCDFGGTAQLVSTNPSYGNCHY------------AKPIDLSIYGARPSGMPSSVIS 210
           P+ +SC+F GTA  +S +PS G+CH+                 L ++G  PS       +
Sbjct: 149 PIPSSCNFDGTAITISADPSLGSCHFPSTSTSESILNVTSEDGLGLFGRIPSHPTPKPEA 208

[Close]

BLAST of CmaCh13G005630 vs. TAIR 10
Match: AT1G09460.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 96.7 bits (239), Expect = 2.5e-20
Identity = 57/134 (42.54%), Postives = 71/134 (52.99%), Query Frame = 0


Query: 52  PLHFTQLVDSTIVNPTTTPGQSVPNSVEPNLKPTTGP------GRSGGGSWCIASSAASP 111
           P   T  V + + NP T    + P+   P   P   P          G SWC+A   AS 
Sbjct: 87  PPTLTPPVTNPVTNPVTQYPPTQPSGTVPVPVPVVAPPVVSNSPSVSGQSWCVAKPGASQ 146

Query: 112 TALQVALDYACGYGSTDCSAIQPG--------VKDHASYAFDDYYQRNPVATSCDFGGTA 171
            +LQ ALDYACG    DCS +Q G        ++ HAS+AF+ YYQ+NP   SCDFGG A
Sbjct: 147 VSLQQALDYACGI--ADCSQLQQGGNCYSPISLQSHASFAFNSYYQKNPSPQSCDFGGAA 206

[Close]

BLAST of CmaCh13G005630 vs. TAIR 10
Match: AT2G30933.2 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 95.9 bits (237), Expect = 4.2e-20
Identity = 59/140 (42.14%), Postives = 76/140 (54.29%), Query Frame = 0


Query: 44  EEDRTIFSPLHFTQLVDSTIVNPTTTPGQSVPNS--------VEPNLKPTT--GPGRSGG 103
           EE++ I +PL           NPTTTP   VPNS          P   P++  G    G 
Sbjct: 29  EEEKDITTPL---------ATNPTTTPTTVVPNSDSDASAVATTPLTIPSSPHGVAYPGD 88

Query: 104 GSWCIASSAASPTALQVALDYACGYGSTDCSAIQPG--------VKDHASYAFDDYYQRN 163
            SWC+A    +  ALQ ALDYACG G  DCS IQ G        ++ HAS+AF+ YYQ+N
Sbjct: 89  QSWCVARENVAKMALQAALDYACGIGGADCSEIQEGGNCYNPNSLRAHASFAFNSYYQKN 148

Query: 164 PVATSCDFGGTAQLVSTNPS 166
           P+ +SC+F GTA  +S +PS
Sbjct: 149 PIPSSCNFDGTAITISADPS 159

[Close]

BLAST of CmaCh13G005630 vs. TAIR 10
Match: AT4G29360.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 92.8 bits (229), Expect = 3.6e-19
Identity = 58/127 (45.67%), Postives = 73/127 (57.48%), Query Frame = 0


Query: 60  DSTIVNPT-----TTPGQSVPNSVEPNLKPTTGPGRSGG-GSWCIASSAASPTALQVALD 119
           ++T V+PT     T+P  S    +  N   T G G  GG   WCIASS AS T LQ ALD
Sbjct: 349 NTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALD 408

Query: 120 YACGYGSTDCSAIQPG--------VKDHASYAFDDYYQRNPVAT-SCDFGGTAQLVSTNP 172
           +ACG G+ DCSA+QP         V  HASYAF+ YYQ++  ++  C F G +  V  +P
Sbjct: 409 WACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDP 468

The following BLAST results are available for this feature:

BLAST of CmaCh13G005630 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYE5	5.0e-18	45.67	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
P52409	6.8e-15	38.64	Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1	[more]
O65399	5.8e-14	47.62	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]
Q9FJU9	2.9e-13	41.32	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]
Q94CD8	4.1e-12	42.22	Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ...	[more]

BLAST of CmaCh13G005630 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ELB1	3.7e-80	90.17	major pollen allergen Ole e 10-like OS=Cucurbita moschata OX=3662 GN=LOC11143434...	[more]
A0A1S3B947	7.2e-76	60.14	PLASMODESMATA CALLOSE-BINDING PROTEIN 4-like OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A0A0M054	1.1e-73	59.79	X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G459490 PE=4 SV=...	[more]
A0A6J1JX26	4.8e-72	58.95	PLASMODESMATA CALLOSE-BINDING PROTEIN 1-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1GUD6	1.8e-71	58.95	PLASMODESMATA CALLOSE-BINDING PROTEIN 1-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]

BLAST of CmaCh13G005630 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7019539.1	2.6e-96	91.09	Glucan endo-1,3-beta-glucosidase 7, partial [Cucurbita argyrosperma subsp. argyr...	[more]
XP_022927548.1	7.6e-80	90.17	major pollen allergen Ole e 10-like [Cucurbita moschata]	[more]
KAG6583926.1	2.2e-79	90.12	Pre-mRNA-processing factor 39, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023519757.1	6.0e-77	89.60	glucan endo-1,3-beta-glucosidase 12-like [Cucurbita pepo subsp. pepo]	[more]
XP_038896033.1	3.9e-76	61.15	PLASMODESMATA CALLOSE-BINDING PROTEIN 4-like [Benincasa hispida]	[more]

BLAST of CmaCh13G005630 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G29380.1	4.0e-26	40.22	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G30933.1	1.2e-22	35.71	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G09460.1	2.5e-20	42.54	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G30933.2	4.2e-20	42.14	Carbohydrate-binding X8 domain superfamily protein	[more]
AT4G29360.1	3.6e-19	45.67	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 95..171 e-value: 5.3E-34 score: 129.0
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 96..157 e-value: 2.5E-12 score: 47.3
None	No IPR available	GENE3D	1.20.58.1040		coord: 93..175 e-value: 6.1E-7 score: 31.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 66..89
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 66..84
None	No IPR available	PANTHER	PTHR31044:SF79	BETA-1,3 GLUCANASE	coord: 12..204
IPR044788	Carbohydrate-binding X8 domain-containing protein, plant	PANTHER	PTHR31044	BETA-1,3 GLUCANASE	coord: 12..204

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh13G005630.1	CmaCh13G005630.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0009506	plasmodesma
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds