Homology
BLAST of CmaCh13G003330 vs. ExPASy Swiss-Prot
Match:
F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)
HSP 1 Score: 829.7 bits (2142), Expect = 2.6e-239
Identity = 427/771 (55.38%), Postives = 528/771 (68.48%), Query Frame = 0
Query: 2 NFTNFILILFWFFRLISPVIAVKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFLG 61
+ ++ +L++ FF SP A+KKSYIV LGSH+H ++S+ L V SH L SF+G
Sbjct: 20 SLSSLLLLVTLFF---SPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVG 79
Query: 62 SSEKAKDAIFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMHL 121
S E AK+AIFYSYK HINGFAAILDE EA + AKHP+V +V PNK ++LHTTHSW FM L
Sbjct: 80 SHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLL 139
Query: 122 EKNGFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTPD 181
KNG + S W KA +G+D IIANLDTGVWPESKSF ++G G VP+RWKG C
Sbjct: 140 AKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEG-YGAVPARWKGRCHKD--- 199
Query: 182 AVPCNRKLIGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSGV 241
VPCNRKLIGA+YFN+G +AY + + RD+ GHGSHTLSTA GN+V G
Sbjct: 200 -VPCNRKLIGARYFNKGYLAYTGLPSNAS-----YETCRDHDGHGSHTLSTAAGNFVPGA 259
Query: 242 SVFGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIGS 301
+VFG G GTA GGSPKARVAAYKVCWP + C+DADI I+ AI DGVDVLS S+G
Sbjct: 260 NVFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGG 319
Query: 302 PPAEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDR----- 361
+Y D IAI SFHA+K G+ VVCSAGNSGP T +N+APW++TVGAS++DR
Sbjct: 320 DAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAF 379
Query: 362 --------------------RKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKIL 421
K+Y LI+ A A + DA+LCK +LD KV GKIL
Sbjct: 380 VELKNGQSFKGTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKIL 439
Query: 422 VCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYI 481
VCL G ++R+DKGMQA AGA GM+LCND+ SG +II+D HVLPAS I Y DG+ + SY+
Sbjct: 440 VCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYL 499
Query: 482 NSRKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGAV 541
+S K+P GY+ P++ +NTKP+P MA+FSSRGPN I+P I+KPD+TAPGVNIIAAF+ A
Sbjct: 500 SSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEAT 559
Query: 542 SPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNT 601
PT DNR P+ T SGTSMSCPH+SG+VGLLK LHP WSPAAI+SAIMT++ +N
Sbjct: 560 GPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNR 619
Query: 602 MNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRV 661
M+D F A PF YGSGH+ P A PGLVYDL+ DYL+FLCA GY +++
Sbjct: 620 RKPMVDES---FKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQL 679
Query: 662 FA-DENFKCPVSGSILNFNYPSIGVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVS 721
FA D + C ++L+FNYPSI V NLTGS+TVTR+LKNVG P Y R R P GV+VS
Sbjct: 680 FAEDPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVS 739
Query: 722 VKPRVLKFWKIGEEKRFELTMTG-AVAEGQIGYGTLIWTDGKHFVKSPIVV 746
V+P+ L F K GE K F++T+ V +G L WTD H+V+SPIVV
Sbjct: 740 VEPKQLTFNKTGEVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVV 774
BLAST of CmaCh13G003330 vs. ExPASy Swiss-Prot
Match:
Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)
HSP 1 Score: 771.2 bits (1990), Expect = 1.1e-221
Identity = 399/774 (51.55%), Postives = 526/774 (67.96%), Query Frame = 0
Query: 5 NFILILFWFFRLISPVIAVK--KSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFLGS 64
+F+L+L ++A K SY+V G+HSH E++ + RV ++H+ LGSF GS
Sbjct: 8 SFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGS 67
Query: 65 SEKAKDAIFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMHLE 124
E+A DAIFYSY HINGFAA LD + A + +KHPEV +V PNKA +LHTT SW+F+ LE
Sbjct: 68 RERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLE 127
Query: 125 KNGFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTPDA 184
N ++ S WRKARFG+D IIANLDTGVWPESKSF ++G+ G +PSRWKG C ++
Sbjct: 128 HNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDAT 187
Query: 185 VPCNRKLIGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSGVS 244
CNRKLIGA+YFN+G A + N + + P RD GHGSHTLSTA G++V GVS
Sbjct: 188 FHCNRKLIGARYFNKGYAAAVGHLNSSFDSP------RDLDGHGSHTLSTAAGDFVPGVS 247
Query: 245 VFGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIGSP 304
+FG G GTAKGGSP+ARVAAYKVCWP CYDAD+ D AI+DG DV+S+S+G
Sbjct: 248 IFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGE 307
Query: 305 PAEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDRR----- 364
P +++D++AI SFHA KK I VVCSAGNSGP+ +T +N+APW +TVGAST+DR
Sbjct: 308 PTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNL 367
Query: 365 ---------------------KLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKIL 424
K YP++ AKA ASA DA LCK +LD K GKIL
Sbjct: 368 VLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKIL 427
Query: 425 VCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYI 484
VCL G + R++KG L G +GM+L N +G ++AD HVLPA+ ++ D AVS YI
Sbjct: 428 VCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYI 487
Query: 485 NSRKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGAV 544
+ K P+ ++ P + + KP+P+MA+FSS+GP++++P+I+KPD+TAPGV++IAA++GAV
Sbjct: 488 SQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAV 547
Query: 545 SPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNT 604
SPT E FD R + + +SGTSMSCPH+SGI GLLK +P WSPAAI+SAIMT+ATI D+
Sbjct: 548 SPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDI 607
Query: 605 MNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRV 664
+ + + ATPF +G+GH+ P A++PGLVYDL DYL FLC+ GY I V
Sbjct: 608 PGPIQNATN---MKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISV 667
Query: 665 FADENFKCPVSG-SILNFNYPSIGVQNLTGS-VTVTRRLKNVGRPGVYRVRVRRPEGVKV 724
F+ NF C S++N NYPSI V NLT S VTV+R +KNVGRP +Y V+V P+GV V
Sbjct: 668 FSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYV 727
Query: 725 SVKPRVLKFWKIGEEKRFELTMT---GAVAEGQIGYGTLIWTDGKHFVKSPIVV 746
+VKP L F K+GE+K F++ + G VA+G + +G L+W+D KH V+SPIVV
Sbjct: 728 AVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYV-FGELVWSDKKHRVRSPIVV 770
BLAST of CmaCh13G003330 vs. ExPASy Swiss-Prot
Match:
I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)
HSP 1 Score: 646.7 bits (1667), Expect = 3.1e-184
Identity = 372/783 (47.51%), Postives = 479/783 (61.17%), Query Frame = 0
Query: 11 FWFFRLISPVIAVKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFLGSSEKAKDAI 70
F F L++ V KK YIV +G+HSHG ++ DL+ DSH+ LLGS GS EKAK+AI
Sbjct: 16 FLFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREKAKEAI 75
Query: 71 FYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFILPF 130
YSY HINGFAA+L+EEEA AK+P V +V +K +LHTT SWEF+ L + G
Sbjct: 76 IYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRRG---QN 135
Query: 131 SPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGC--TDKTPDAV--PCN 190
S W+K RFG++ II N+DTGVWPES+SF ++G G VPS+W+GG +K P ++ CN
Sbjct: 136 SAWQKGRFGENTIIGNIDTGVWPESQSFSDKG-YGTVPSKWRGGLCQINKLPGSMKNTCN 195
Query: 191 RKLIGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSGVSVFGS 250
RKLIGA+Y+N+ A+ +L ++++ RD+VGHG+HTLSTAGGN+V G VF
Sbjct: 196 RKLIGARYYNKAFEAH------NGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAV 255
Query: 251 GIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIGSP---P 310
G GTAKGGSP+ARVAAYKVCW + CY AD+ IDQAI DGVDV+++S G
Sbjct: 256 GNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVT 315
Query: 311 AE-YYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDR------ 370
AE + D I+I +FHA+ K I +V SAGN GP+ T N+APW+ T+ ASTLDR
Sbjct: 316 AEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNL 375
Query: 371 ------------------RKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKILVC 430
+ + LI AK A+ DA LC+ TLDR+KVNGKI++C
Sbjct: 376 TINNQLIEGASLFVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIVLC 435
Query: 431 LTGGSSR-IDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVL-----PASHISYNDGQAV 490
G + + +G++A+ AGA GMIL N +G + A+ HV P
Sbjct: 436 TREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGVK 495
Query: 491 SSYINSRKNPM--GYLIPPS---SKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVN 550
++ I +P+ G I S + KP+P+MA+FSSRGPN I P I+KPDVTAPGVN
Sbjct: 496 TTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVN 555
Query: 551 IIAAFSGAVSPTGEPFDNRT-VPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAI 610
I+AA+S S + DNR + + GTSMSCPH SGI GLLK HP WSPAAIKSAI
Sbjct: 556 ILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAI 615
Query: 611 MTSATISDNTMNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGLVYDLSPNDYLEFLCA 670
MT+AT DNT + D A A F YGSGH+ P AI+PGLVYDLS DYL FLCA
Sbjct: 616 MTTATTLDNTNRPIQDAFDKTLADA--FAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCA 675
Query: 671 SGYKEKNIRVF-ADENFKCPVSGSILNFNYPSIGVQNL-TGSVTVTRRLKNVGRPGVYRV 730
SGY ++ I + F C S S+ + NYPSI + NL VT+ R + NVG P Y V
Sbjct: 676 SGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPSTYTV 735
Query: 731 RVRRPEGVKVSVKPRVLKFWKIGEEKRFELTM--TGAVAEGQIGYGTLIWTDGKHFVKSP 746
R P G ++V P L F KIGE K F++ + + A + +G L WTDGKH V+SP
Sbjct: 736 STRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVRSP 786
BLAST of CmaCh13G003330 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 544.3 bits (1401), Expect = 2.2e-153
Identity = 328/781 (42.00%), Postives = 455/781 (58.26%), Query Frame = 0
Query: 1 MNFTNFILILFWFFRLISPVIAVKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFL 60
++ T F L+L F +S + + +YIV ++ + D H S L
Sbjct: 6 LSSTAFFLLLCLGFCHVSSSSSDQGTYIV---------HMAKSQMPSSFDLHSNWYDSSL 65
Query: 61 GSSEKAKDAIFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMH 120
S + + + Y+Y+N I+GF+ L +EEA P V +VLP ELHTT + F+
Sbjct: 66 RSISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLG 125
Query: 121 LEKNGFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTP 180
L+++ L + +A DV++ LDTGVWPESKS+ ++G G +PS WKGGC T
Sbjct: 126 LDEHTADL----FPEAGSYSDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEAGTN 185
Query: 181 -DAVPCNRKLIGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVS 240
A CNRKLIGA++F +G Y + DE S RD GHG+HT STA G+ V
Sbjct: 186 FTASLCNRKLIGARFFARG---YESTMGPIDE-SKESRSPRDDDGHGTHTSSTAAGSVVE 245
Query: 241 GVSVFGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSI 300
G S+ G GTA+G +P+ARVA YKVCW GGC+ +DI ID+AI D V+VLS+S+
Sbjct: 246 GASLLGYASGTARGMAPRARVAVYKVCW----LGGCFSSDILAAIDKAIADNVNVLSMSL 305
Query: 301 GSPPAEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDR--- 360
G ++YY D +AI +F A+++GI V CSAGN+GPS ++ +N+APWI TVGA TLDR
Sbjct: 306 GGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFP 365
Query: 361 -----------------------RKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNG 420
KL P I A ++A + LC TL KV G
Sbjct: 366 ALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNA----SNATNGNLCMTGTLIPEKVKG 425
Query: 421 KILVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVS 480
KI++C G ++R+ KG AG VGMIL N +G +++AD H+LPA+ + G +
Sbjct: 426 KIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIR 485
Query: 481 SYINSRKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFS 540
Y+ + NP + + V KPSP++AAFSSRGPN I+P I+KPD+ APGVNI+AA++
Sbjct: 486 HYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWT 545
Query: 541 GAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSA--T 600
GA PTG D+R V + +SGTSMSCPHVSG+ LLK++HPEWSPAAI+SA+MT+A T
Sbjct: 546 GAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKT 605
Query: 601 ISDNTMNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKE 660
D L + G P+TPF +G+GH+ PT A +PGL+YDL+ DYL FLCA Y
Sbjct: 606 YKDGKPLLDIATGK----PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTS 665
Query: 661 KNIRVFADENFKCPVSG--SILNFNYPSIGVQ-NLTGSVTVTRRLKNVGRPGVYRVRV-R 720
IR + N+ C S S+ + NYPS V + G+ TR + +VG G Y V+V
Sbjct: 666 PQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTS 725
Query: 721 RPEGVKVSVKPRVLKFWKIGEEKRFELTMT--GAVAEGQIGYGTLIWTDGKHFVKSPIVV 747
GVK+SV+P VL F + E+K + +T T + G +G++ W+DGKH V SP+ +
Sbjct: 726 ETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 755
BLAST of CmaCh13G003330 vs. ExPASy Swiss-Prot
Match:
A9JQS7 (Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1)
HSP 1 Score: 535.0 bits (1377), Expect = 1.3e-150
Identity = 323/774 (41.73%), Postives = 452/774 (58.40%), Query Frame = 0
Query: 5 NFILILFWFFRLISPVIAVKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFLGSSE 64
+F L+LF + L+ + K YIV +G SH + + VV ++H++L S GS
Sbjct: 8 SFTLLLFVGYTLVHG--STPKHYIVYMGDRSHP------NSESVVRANHEILASVTGSLN 67
Query: 65 KAKDAIFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMHLEKN 124
AK A + Y GF+A++ E+A K A H V +V +K +LHTTHSW+F+ L+
Sbjct: 68 DAKAAAIHHYSRSFQGFSAMITPEQAKKLADHNSVVSVFESKMNKLHTTHSWDFLGLDT- 127
Query: 125 GFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGC-TDKTPDAV 184
+ +P +VI+ +D+GVWPES+SF + G+ G VP ++KG C T
Sbjct: 128 --VYKNNP-SALDSASNVIVGVIDSGVWPESESFNDYGL-GPVPEKFKGECVTGDNFTLA 187
Query: 185 PCNRKLIGAKYFNQGVIAYL-KSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSGVS 244
CN+K+IGA+++++G+ A + +N+ D + S RD GHG+HT ST G+ VS VS
Sbjct: 188 NCNKKIIGARFYSKGLEAEIGPLENIVDS--IFFRSPRDSDGHGTHTASTIAGSIVSNVS 247
Query: 245 VFGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIGSP 304
+FG GTA+GG+P AR++ YK CW G C DAD+F +D AI+DGVD+LSLS+G
Sbjct: 248 LFGMAKGTARGGAPSARLSIYKACW----FGFCSDADVFAAMDDAIHDGVDILSLSLGPD 307
Query: 305 PAE--YYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDR---- 364
P + Y+++ I++ +FHA +KGI V SAGNS TA N+APWI TV AST+DR
Sbjct: 308 PPQPLYFENAISVGAFHAFQKGILVSASAGNS-VFPRTACNVAPWIFTVAASTVDREFRS 367
Query: 365 --------------------RKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKIL 424
Y LI G+ A A +A +A CK TLD + + GKI+
Sbjct: 368 DIYLGNSKVLKGLSLNPIKMEGSYGLIYGSAAAAAGDAALNASFCKEHTLDPTLIKGKIV 427
Query: 425 VCLTG--GSSRIDKGMQAVLAGAVGMILC--NDRFSGFKIIADLHVLPASHISYNDGQAV 484
+C +R +K + G VGMIL N R GF+ V+P++ I + + +
Sbjct: 428 ICTVEKFTDNRREKAIIIKQGGGVGMILIDHNARDVGFQF-----VIPSTMIGQDAVEEL 487
Query: 485 SSYINSRKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAF 544
+Y+ + KNP + P + V TKP+P AAFSS GPN+I+P+IIKPD+T PGVNI+AA+
Sbjct: 488 QAYMKTEKNPTATIFPTLTLVGTKPAPESAAFSSVGPNIITPDIIKPDITGPGVNILAAW 547
Query: 545 SGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATI 604
S T + ++V Y +SGTSMSCPH+S I ++K+ HP WSPAAI SAIMTSAT+
Sbjct: 548 SPVA--TEATVEQKSVNYNIISGTSMSCPHISAISAIIKSHHPSWSPAAIMSAIMTSATV 607
Query: 605 SDNTMNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEK 664
DNT +L+ G P ATPF YGSGH++P +++PGLVYD S D L FLC++G
Sbjct: 608 MDNTHSLI--GRDPNGTQATPFDYGSGHVNPVASLNPGLVYDFSSQDVLNFLCSNGASPA 667
Query: 665 NIRVFADENFKCPVSGSI-LNFNYPSIGVQNLTGSVTVTRRLKNVGR-PGVYRVRVRRPE 724
++ E +C S + NFNYPSIGV NL GS++V R + G+ P Y V RP
Sbjct: 668 QLKNLTGELTQCQKSPTASYNFNYPSIGVSNLNGSLSVYRTVTYYGQEPTEYFASVERPS 727
Query: 725 GVKVSVKPRVLKFWKIGEEKRFELTMTG-AVAEGQIGYGTLIWTDGKHFVKSPI 744
GV V V P LKFWK GE+ F + T + G +G L W +GK V+SPI
Sbjct: 728 GVIVRVTPAKLKFWKAGEKITFRIDFTPFKNSNGNFVFGALTWNNGKQRVRSPI 752
BLAST of CmaCh13G003330 vs. ExPASy TrEMBL
Match:
A0A6J1I7Q4 (subtilisin-like protease SBT5.4 OS=Cucurbita maxima OX=3661 GN=LOC111471617 PE=3 SV=1)
HSP 1 Score: 1414.1 bits (3659), Expect = 0.0e+00
Identity = 718/775 (92.65%), Postives = 718/775 (92.65%), Query Frame = 0
Query: 1 MNFTNFILILFWFFRLISPVIAVKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFL 60
MNFTNFILILFWFFRLISPVIAVKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFL
Sbjct: 1 MNFTNFILILFWFFRLISPVIAVKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFL 60
Query: 61 GSSEKAKDAIFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMH 120
GSSEKAKDAIFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMH
Sbjct: 61 GSSEKAKDAIFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMH 120
Query: 121 LEKNGFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTP 180
LEKNGFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTP
Sbjct: 121 LEKNGFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTP 180
Query: 181 DAVPCNRKLIGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSG 240
DAVPCNRKLIGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSG
Sbjct: 181 DAVPCNRKLIGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSG 240
Query: 241 VSVFGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIG 300
VSVFGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIG
Sbjct: 241 VSVFGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIG 300
Query: 301 SPPAEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDR---- 360
SPPAEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDR
Sbjct: 301 SPPAEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQA 360
Query: 361 ---------------------RKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKI 420
RKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKI
Sbjct: 361 PIELGNGQRFWGSSLSRPLAGRKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKI 420
Query: 421 LVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY 480
LVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY
Sbjct: 421 LVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY 480
Query: 481 INSRKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGA 540
INSRKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGA
Sbjct: 481 INSRKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGA 540
Query: 541 VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN 600
VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN
Sbjct: 541 VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN 600
Query: 601 TMNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIR 660
TMNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGL
Sbjct: 601 TMNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGL------------------------ 660
Query: 661 VFADENFKCPVSGSILNFNYPSIGVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVS 720
CPVSGSILNFNYPSIGVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVS
Sbjct: 661 --------CPVSGSILNFNYPSIGVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVS 720
Query: 721 VKPRVLKFWKIGEEKRFELTMTGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 751
VKPRVLKFWKIGEEKRFELTMTGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF
Sbjct: 721 VKPRVLKFWKIGEEKRFELTMTGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 743
BLAST of CmaCh13G003330 vs. ExPASy TrEMBL
Match:
A0A6J1EK27 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434038 PE=3 SV=1)
HSP 1 Score: 1392.5 bits (3603), Expect = 0.0e+00
Identity = 690/753 (91.63%), Postives = 708/753 (94.02%), Query Frame = 0
Query: 23 VKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFA 82
+ +SYIVLLGSHSHGLEVS ID+QRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFA
Sbjct: 16 ILQSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFA 75
Query: 83 AILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFILPFSPWRKARFGKDV 142
AILDEEEAT AKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFI PFSPWRKARFGKDV
Sbjct: 76 AILDEEEATNLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFIFPFSPWRKARFGKDV 135
Query: 143 IIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTPDAVPCNRKLIGAKYFNQGVIAY 202
IIANLDTGVWPESKSFGEQGI GGVPSRWKGGC DKTPDAVPCN+KLIGAKYFNQG+IAY
Sbjct: 136 IIANLDTGVWPESKSFGEQGITGGVPSRWKGGCMDKTPDAVPCNKKLIGAKYFNQGLIAY 195
Query: 203 LKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSGVSVFGSGIGTAKGGSPKARVAA 262
LKS NLTDELPLIVNSTRDYVGHG+HTLSTAGG+YVSGVSVFGSGIGTAKGGSPKARVAA
Sbjct: 196 LKSHNLTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAA 255
Query: 263 YKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIGSPPAEYYDDTIAIASFHALKKG 322
YKVCWPF N GGCYDADIFDG DQAI+DGVDVLSLSIGSPP EYYDDTIAIASFHALKKG
Sbjct: 256 YKVCWPFRNGGGCYDADIFDGFDQAIHDGVDVLSLSIGSPPDEYYDDTIAIASFHALKKG 315
Query: 323 IPVVCSAGNSGPSMATATNIAPWILTVGASTLD-------------------------RR 382
IPVVCSAGNSGPSMATATNIAPWILTVGASTLD RR
Sbjct: 316 IPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARR 375
Query: 383 KLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKILVCLTGGSSRIDKGMQAVLAGA 442
KLYPLITGAQAKATT SADDAMLCKPKTLD SKVNGKILVCLTGGSSRIDKGMQAVLAGA
Sbjct: 376 KLYPLITGAQAKATTTSADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGA 435
Query: 443 VGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLIPPSSKVNTKP 502
VGMILCNDRFSGFKIIADLHVLPASHISYNDGQA+SSYINSRKNPMGYLIPPSSKVNTKP
Sbjct: 436 VGMILCNDRFSGFKIIADLHVLPASHISYNDGQAISSYINSRKNPMGYLIPPSSKVNTKP 495
Query: 503 SPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGAVSPTGEPFDNRTVPYITMSGTS 562
SP MAAFSSRGPN++SPEIIKPDVTAPGV+IIAAFSGAVSPTGEPFDNRTVPYITMSGTS
Sbjct: 496 SPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTS 555
Query: 563 MSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLMLDGGSPIFAPATPFIYG 622
MSCPHVSGIVGLLKALHP+WSPAAIKSAIMTSATISDNTMNL+LDGGSPIFAPATPFIYG
Sbjct: 556 MSCPHVSGIVGLLKALHPQWSPAAIKSAIMTSATISDNTMNLILDGGSPIFAPATPFIYG 615
Query: 623 SGHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFADENFKCPVSGSILNFNYPS 682
SGHIHP GAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFA+EN+KCPVSGSILNFNYPS
Sbjct: 616 SGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFAEENYKCPVSGSILNFNYPS 675
Query: 683 IGVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMT 742
IGVQNLTG VT+TRRLKNVGRPGVYRVRVRRP+GVKV VKPRVLKF KIGEEKRFELTMT
Sbjct: 676 IGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVLVKPRVLKFRKIGEEKRFELTMT 735
Query: 743 GAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 751
G VAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF
Sbjct: 736 GVVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 768
BLAST of CmaCh13G003330 vs. ExPASy TrEMBL
Match:
A0A6J1EG71 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434037 PE=3 SV=1)
HSP 1 Score: 1379.0 bits (3568), Expect = 0.0e+00
Identity = 683/752 (90.82%), Postives = 706/752 (93.88%), Query Frame = 0
Query: 24 KKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAA 83
K+SYIVLLGSHSHGLEVS +DLQRVVDSHHKLLGS LGSSEKAKDAIF+SYKNHINGFAA
Sbjct: 251 KESYIVLLGSHSHGLEVSAVDLQRVVDSHHKLLGSLLGSSEKAKDAIFHSYKNHINGFAA 310
Query: 84 ILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFILPFSPWRKARFGKDVI 143
IL+EEEATK AKHPEVAAVLPNKAKELHTTHSWEFMHLEKNG +LPFSPWRKARFG+DVI
Sbjct: 311 ILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGVVLPFSPWRKARFGRDVI 370
Query: 144 IANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTPDAVPCNRKLIGAKYFNQGVIAYL 203
IANLDTGVWPESKSFGEQGIVGGVPSRWKGGC DKTPDAVPCNRKLIGAKYFNQG+IAYL
Sbjct: 371 IANLDTGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDAVPCNRKLIGAKYFNQGLIAYL 430
Query: 204 KSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSGVSVFGSGIGTAKGGSPKARVAAY 263
KS NLTDEL LIVNSTRDYVGHG+HTLSTAGG+YVSGVSVFGSGIGTAKGGSPKARVAAY
Sbjct: 431 KSHNLTDELSLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAY 490
Query: 264 KVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIGSPPAEYYDDTIAIASFHALKKGI 323
KVCWPFHNSGGCYD D+ DG DQAI+DGVDVLSLSIGSPPAEYYDDTIAIASF A+KKGI
Sbjct: 491 KVCWPFHNSGGCYDIDVVDGFDQAIHDGVDVLSLSIGSPPAEYYDDTIAIASFLAIKKGI 550
Query: 324 PVVCSAGNSGPSMATATNIAPWILTVGASTLD-------------------------RRK 383
PVVCSAGNSGPSMA+A+NIAPWILTVGASTLD RRK
Sbjct: 551 PVVCSAGNSGPSMASASNIAPWILTVGASTLDREFQAPIELGNGQRFWGSSLSRPLARRK 610
Query: 384 LYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKILVCLTGGSSRIDKGMQAVLAGAV 443
LYPLITGAQAKATTASADDAMLCKPKTLD SKVNGKILVCLTGGSSRIDKGMQAVLAGAV
Sbjct: 611 LYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAV 670
Query: 444 GMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLIPPSSKVNTKPS 503
GMILCNDRFSGFKIIADLHVLPASHI+YNDGQAV SYINS KNPMGYLIPPSSKVNTKPS
Sbjct: 671 GMILCNDRFSGFKIIADLHVLPASHINYNDGQAVFSYINSWKNPMGYLIPPSSKVNTKPS 730
Query: 504 PIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGAVSPTGEPFDNRTVPYITMSGTSM 563
P MAAFSSRGPNM+SPEIIKPDVTAPGVNIIAAFSGAVSPT EPFDNRTVPYITMSGTSM
Sbjct: 731 PTMAAFSSRGPNMVSPEIIKPDVTAPGVNIIAAFSGAVSPTREPFDNRTVPYITMSGTSM 790
Query: 564 SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLMLDGGSPIFAPATPFIYGS 623
SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSA ISDNTMNL+LDGGSP+FAPATPFIYGS
Sbjct: 791 SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSAIISDNTMNLILDGGSPMFAPATPFIYGS 850
Query: 624 GHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFADENFKCPVSGSILNFNYPSI 683
GHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFADENFKCP+S SI NFNYPSI
Sbjct: 851 GHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFADENFKCPISCSIFNFNYPSI 910
Query: 684 GVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMTG 743
GVQNLTGSVT+ RRLKNVGRPGVYRVRVR+PEGVKVSVKPRVLKFWKIGEEKRFELTMTG
Sbjct: 911 GVQNLTGSVTLKRRLKNVGRPGVYRVRVRQPEGVKVSVKPRVLKFWKIGEEKRFELTMTG 970
Query: 744 AVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 751
A+AEGQIGYGTLIWTDGKHFV+SPIVVSSG F
Sbjct: 971 AMAEGQIGYGTLIWTDGKHFVRSPIVVSSGLF 1002
BLAST of CmaCh13G003330 vs. ExPASy TrEMBL
Match:
A0A6J1EG66 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434032 PE=3 SV=1)
HSP 1 Score: 1332.0 bits (3446), Expect = 0.0e+00
Identity = 667/772 (86.40%), Postives = 696/772 (90.16%), Query Frame = 0
Query: 4 TNFILILFWFFRLISPVIAVKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFLGSS 63
TNFI +LF FF LISPVIAVKKSYIVLLGSHSHGLEVS +DLQR VDSHHKLLGSFLGSS
Sbjct: 5 TNFIFMLFGFFWLISPVIAVKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSS 64
Query: 64 EKAKDAIFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMHLEK 123
KAKDAIFYSYKNHINGFAAIL+EEEATK AKHPEVAAVLPNKAKELHTTHSWE MHLEK
Sbjct: 65 GKAKDAIFYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWESMHLEK 124
Query: 124 NGFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTPDAV 183
N I SPWRKARFG+DVIIANLD+GVWPESKSFGEQGIVGGVPSRWKGGC DKTPD V
Sbjct: 125 NDVIPSSSPWRKARFGEDVIIANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGV 184
Query: 184 PCNRKLIGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSGVSV 243
PCNRKLIGAKYFNQG +AYLKSQNL +ELPLIVNSTRDY GHGSHTLSTAGG+YVS VSV
Sbjct: 185 PCNRKLIGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSV 244
Query: 244 FGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIGSPP 303
FGSGIGTAKGGSPKARVAAYKVCWP N GGC+DAD+ + D AI+DGVDVLSLSIGSPP
Sbjct: 245 FGSGIGTAKGGSPKARVAAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPP 304
Query: 304 AEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDR------- 363
AEYYDD IAI SFHALKKGIPVVCSAGNSGPSMATA+NIAPWILTVGASTLDR
Sbjct: 305 AEYYDDIIAIGSFHALKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIE 364
Query: 364 ------------------RKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKILVC 423
RKLYPLITGAQAKATTA A D++LCKP+TLD SKV GKILVC
Sbjct: 365 LGNGQRFTGSSLSNPLAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVC 424
Query: 424 LTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINS 483
L GGSSRIDKGMQA+LAGAVGMILCNDR SGF+I+ADLHVLP SHI+YNDGQAV SYI S
Sbjct: 425 LRGGSSRIDKGMQALLAGAVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYS 484
Query: 484 RKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGAVSP 543
KNPMGYLIPPSSKVNTKPSP MAAFSSRGPN++SPEIIKPDVTAPGVNIIAAFSGAVSP
Sbjct: 485 TKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVNIIAAFSGAVSP 544
Query: 544 TGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMN 603
TGEPFDNR VPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSA +SDNTMN
Sbjct: 545 TGEPFDNRAVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMN 604
Query: 604 LMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFA 663
LMLDGGSPIFAPATPF+YGSGHI PTGAIDPGLVYDLSPNDYLEFLCASGY EKN+RVF
Sbjct: 605 LMLDGGSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFT 664
Query: 664 DENFKCPVSGSILNFNYPSIGVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKP 723
D NFKCPVSGSILNFNYPSIGVQNLTGSVTV+RRLKNVG PGVYRVRV++PEGVKVSVKP
Sbjct: 665 DGNFKCPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKP 724
Query: 724 RVLKFWKIGEEKRFELTMTGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 751
VLKF KIGEEK FELTMTGAVAEGQIGYGTLIWTD KHFV+SPIVVSS FF
Sbjct: 725 NVLKFEKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF 776
BLAST of CmaCh13G003330 vs. ExPASy TrEMBL
Match:
A0A6J1EGQ8 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434031 PE=3 SV=1)
HSP 1 Score: 1297.0 bits (3355), Expect = 0.0e+00
Identity = 643/753 (85.39%), Postives = 680/753 (90.31%), Query Frame = 0
Query: 23 VKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFA 82
+++SYIVLLGSH+HGLEVS +DLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFA
Sbjct: 1 MEESYIVLLGSHAHGLEVSAVDLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFA 60
Query: 83 AILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFILPFSPWRKARFGKDV 142
AILDEEEATK AKHPE+AAVLPNKAKELHTT SWEFMHLEKNG P SPWRKAR G++V
Sbjct: 61 AILDEEEATKLAKHPEIAAVLPNKAKELHTTRSWEFMHLEKNGVSPPSSPWRKARSGQNV 120
Query: 143 IIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTPDAVPCNRKLIGAKYFNQGVIAY 202
IIANLDTGVWPESKSFGE GIVGGVPS+WKGGC DKTPDAVPCN+KLIGAKYFN GVIAY
Sbjct: 121 IIANLDTGVWPESKSFGEHGIVGGVPSKWKGGCMDKTPDAVPCNKKLIGAKYFNHGVIAY 180
Query: 203 LKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSGVSVFGSGIGTAKGGSPKARVAA 262
LKS+NLT EL IVNSTRDY GHGSHTLSTAGG+YVSGVSVFGSGIGTAKGGSPKA VAA
Sbjct: 181 LKSENLTKELSFIVNSTRDYEGHGSHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKAHVAA 240
Query: 263 YKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIGSPPAEYYDDTIAIASFHALKKG 322
YKVCWP H++GGC+DADI D AI+DGVDVLS+S+GSPPAEYY+D IAIASFHALKKG
Sbjct: 241 YKVCWPLHDTGGCFDADIAKAFDHAIHDGVDVLSVSLGSPPAEYYNDIIAIASFHALKKG 300
Query: 323 IPVVCSAGNSGPSMATATNIAPWILTVGASTLDR-------------------------R 382
IPVVCSAGN GPSMAT +N APWILTVGASTLDR R
Sbjct: 301 IPVVCSAGNDGPSMATVSNTAPWILTVGASTLDREFQAPIELRNGKHFKGSSLSGPLSGR 360
Query: 383 KLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKILVCLTGGSSRIDKGMQAVLAGA 442
KLYPLITGAQAKATTAS DDAMLCKPKTLD SKV GKILVCL G SSR+DKG+QA+LAGA
Sbjct: 361 KLYPLITGAQAKATTASTDDAMLCKPKTLDHSKVKGKILVCLRGDSSRVDKGVQALLAGA 420
Query: 443 VGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLIPPSSKVNTKP 502
VGMILCNDR SGF+IIAD HVLPASHISYNDGQAVSSYINS KNPMG+LIPP SKVNTKP
Sbjct: 421 VGMILCNDRLSGFEIIADPHVLPASHISYNDGQAVSSYINSTKNPMGHLIPPLSKVNTKP 480
Query: 503 SPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGAVSPTGEPFDNRTVPYITMSGTS 562
SP MAAFSSRGPNM+SPEIIKPDVTAPGVNIIAAFSG+VSPTGEPFDNRTVPYITMSGTS
Sbjct: 481 SPTMAAFSSRGPNMVSPEIIKPDVTAPGVNIIAAFSGSVSPTGEPFDNRTVPYITMSGTS 540
Query: 563 MSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLMLDGGSPIFAPATPFIYG 622
MSCPHVSGIVGL+KALHP+WSPAAIKSAIMTSA I DNTMN+MLDGGSPIFA AT FI+G
Sbjct: 541 MSCPHVSGIVGLVKALHPKWSPAAIKSAIMTSARIRDNTMNIMLDGGSPIFASATAFIFG 600
Query: 623 SGHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFADENFKCPVSGSILNFNYPS 682
SGHI PTGAIDPGLVYDLSPNDYLEFLCASGYKEKN+RVFAD NFKCPVS SILNFNYPS
Sbjct: 601 SGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNLRVFADGNFKCPVSSSILNFNYPS 660
Query: 683 IGVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMT 742
IGVQNLTG+VT+TRRLKNVGRPGVYRVRV++PEGVKVSVKP VLKF KIGEEKRFELTMT
Sbjct: 661 IGVQNLTGNVTLTRRLKNVGRPGVYRVRVQQPEGVKVSVKPSVLKFEKIGEEKRFELTMT 720
Query: 743 GAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 751
G VA GQIGYGTLIWTDGKH V+SPIVVSSGFF
Sbjct: 721 GVVANGQIGYGTLIWTDGKHLVRSPIVVSSGFF 753
BLAST of CmaCh13G003330 vs. NCBI nr
Match:
KAG6583696.1 (Subtilisin-like protease 5.4, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1420.2 bits (3675), Expect = 0.0e+00
Identity = 707/775 (91.23%), Postives = 723/775 (93.29%), Query Frame = 0
Query: 1 MNFTNFILILFWFFRLISPVIAVKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFL 60
MN TNFILILFWFF LISPV+AVKKSYIVLLGSHSHGLEVS ID+QRVVDSHHKLLGSFL
Sbjct: 1 MNLTNFILILFWFFPLISPVVAVKKSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFL 60
Query: 61 GSSEKAKDAIFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMH 120
GS EKAKDAIFYSYKNHINGFAAILDEEEAT AKHPEVA VLPNKA+ELHTTHSWEFMH
Sbjct: 61 GSFEKAKDAIFYSYKNHINGFAAILDEEEATNLAKHPEVATVLPNKARELHTTHSWEFMH 120
Query: 121 LEKNGFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTP 180
LEKNGFI PFSPWRKARFGKDVIIANLDTGVWPESKSFGE GI GGVPSRWKGGCTDKTP
Sbjct: 121 LEKNGFIFPFSPWRKARFGKDVIIANLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKTP 180
Query: 181 DAVPCNRKLIGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSG 240
DAVPCN+KLIGAKYFNQGVIAYLKS NLTDELPLI NSTRDYVGHG+HTLSTAGG+YVSG
Sbjct: 181 DAVPCNKKLIGAKYFNQGVIAYLKSHNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSG 240
Query: 241 VSVFGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIG 300
VSVFGSGIGTAKGGSPKARVAAYKVCWPF N GGCYDADIFDG DQAIYDGVDVLSLSIG
Sbjct: 241 VSVFGSGIGTAKGGSPKARVAAYKVCWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIG 300
Query: 301 SPPAEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLD----- 360
SPP EYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLD
Sbjct: 301 SPPDEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQA 360
Query: 361 --------------------RRKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKI 420
RRKLYPLITGAQAKATTASADDAMLCKPKTLD SKVNGKI
Sbjct: 361 PIELGNGQRFWGLSLSRPLARRKLYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKI 420
Query: 421 LVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY 480
LVCLTGGSSRIDKGMQAV+AGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY
Sbjct: 421 LVCLTGGSSRIDKGMQAVVAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY 480
Query: 481 INSRKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGA 540
INSRKNPMGYLIPPSSKVNTKPSP MAAFSSRGPN++SPEIIKPDVTAPGV+IIAAFSGA
Sbjct: 481 INSRKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGA 540
Query: 541 VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN 600
VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN
Sbjct: 541 VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN 600
Query: 601 TMNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIR 660
T NL+LDGGSPIFAPATPFIYGSGHIHP GAIDPGLVYDLSPNDYLEFLCASGYK KN R
Sbjct: 601 TRNLILDGGSPIFAPATPFIYGSGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKAKNFR 660
Query: 661 VFADENFKCPVSGSILNFNYPSIGVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVS 720
VF ++NFKCP+SGSILNFNYPSIGVQNLTG VT+TRRLKNVGRPGVYRVRVRRP+GVKV
Sbjct: 661 VFGEKNFKCPISGSILNFNYPSIGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVL 720
Query: 721 VKPRVLKFWKIGEEKRFELTMTGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 751
VKPRVLKF KIGEEKRFELTM GAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF
Sbjct: 721 VKPRVLKFRKIGEEKRFELTMIGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 775
BLAST of CmaCh13G003330 vs. NCBI nr
Match:
XP_022973086.1 (subtilisin-like protease SBT5.4 [Cucurbita maxima])
HSP 1 Score: 1414.1 bits (3659), Expect = 0.0e+00
Identity = 718/775 (92.65%), Postives = 718/775 (92.65%), Query Frame = 0
Query: 1 MNFTNFILILFWFFRLISPVIAVKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFL 60
MNFTNFILILFWFFRLISPVIAVKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFL
Sbjct: 1 MNFTNFILILFWFFRLISPVIAVKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFL 60
Query: 61 GSSEKAKDAIFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMH 120
GSSEKAKDAIFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMH
Sbjct: 61 GSSEKAKDAIFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMH 120
Query: 121 LEKNGFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTP 180
LEKNGFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTP
Sbjct: 121 LEKNGFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTP 180
Query: 181 DAVPCNRKLIGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSG 240
DAVPCNRKLIGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSG
Sbjct: 181 DAVPCNRKLIGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSG 240
Query: 241 VSVFGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIG 300
VSVFGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIG
Sbjct: 241 VSVFGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIG 300
Query: 301 SPPAEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDR---- 360
SPPAEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDR
Sbjct: 301 SPPAEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQA 360
Query: 361 ---------------------RKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKI 420
RKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKI
Sbjct: 361 PIELGNGQRFWGSSLSRPLAGRKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKI 420
Query: 421 LVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY 480
LVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY
Sbjct: 421 LVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY 480
Query: 481 INSRKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGA 540
INSRKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGA
Sbjct: 481 INSRKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGA 540
Query: 541 VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN 600
VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN
Sbjct: 541 VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN 600
Query: 601 TMNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIR 660
TMNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGL
Sbjct: 601 TMNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGL------------------------ 660
Query: 661 VFADENFKCPVSGSILNFNYPSIGVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVS 720
CPVSGSILNFNYPSIGVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVS
Sbjct: 661 --------CPVSGSILNFNYPSIGVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVS 720
Query: 721 VKPRVLKFWKIGEEKRFELTMTGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 751
VKPRVLKFWKIGEEKRFELTMTGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF
Sbjct: 721 VKPRVLKFWKIGEEKRFELTMTGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 743
BLAST of CmaCh13G003330 vs. NCBI nr
Match:
XP_022927103.1 (subtilisin-like protease SBT5.4 [Cucurbita moschata])
HSP 1 Score: 1392.5 bits (3603), Expect = 0.0e+00
Identity = 690/753 (91.63%), Postives = 708/753 (94.02%), Query Frame = 0
Query: 23 VKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFA 82
+ +SYIVLLGSHSHGLEVS ID+QRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFA
Sbjct: 16 ILQSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFA 75
Query: 83 AILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFILPFSPWRKARFGKDV 142
AILDEEEAT AKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFI PFSPWRKARFGKDV
Sbjct: 76 AILDEEEATNLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFIFPFSPWRKARFGKDV 135
Query: 143 IIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTPDAVPCNRKLIGAKYFNQGVIAY 202
IIANLDTGVWPESKSFGEQGI GGVPSRWKGGC DKTPDAVPCN+KLIGAKYFNQG+IAY
Sbjct: 136 IIANLDTGVWPESKSFGEQGITGGVPSRWKGGCMDKTPDAVPCNKKLIGAKYFNQGLIAY 195
Query: 203 LKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSGVSVFGSGIGTAKGGSPKARVAA 262
LKS NLTDELPLIVNSTRDYVGHG+HTLSTAGG+YVSGVSVFGSGIGTAKGGSPKARVAA
Sbjct: 196 LKSHNLTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAA 255
Query: 263 YKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIGSPPAEYYDDTIAIASFHALKKG 322
YKVCWPF N GGCYDADIFDG DQAI+DGVDVLSLSIGSPP EYYDDTIAIASFHALKKG
Sbjct: 256 YKVCWPFRNGGGCYDADIFDGFDQAIHDGVDVLSLSIGSPPDEYYDDTIAIASFHALKKG 315
Query: 323 IPVVCSAGNSGPSMATATNIAPWILTVGASTLD-------------------------RR 382
IPVVCSAGNSGPSMATATNIAPWILTVGASTLD RR
Sbjct: 316 IPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARR 375
Query: 383 KLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKILVCLTGGSSRIDKGMQAVLAGA 442
KLYPLITGAQAKATT SADDAMLCKPKTLD SKVNGKILVCLTGGSSRIDKGMQAVLAGA
Sbjct: 376 KLYPLITGAQAKATTTSADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGA 435
Query: 443 VGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLIPPSSKVNTKP 502
VGMILCNDRFSGFKIIADLHVLPASHISYNDGQA+SSYINSRKNPMGYLIPPSSKVNTKP
Sbjct: 436 VGMILCNDRFSGFKIIADLHVLPASHISYNDGQAISSYINSRKNPMGYLIPPSSKVNTKP 495
Query: 503 SPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGAVSPTGEPFDNRTVPYITMSGTS 562
SP MAAFSSRGPN++SPEIIKPDVTAPGV+IIAAFSGAVSPTGEPFDNRTVPYITMSGTS
Sbjct: 496 SPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTS 555
Query: 563 MSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLMLDGGSPIFAPATPFIYG 622
MSCPHVSGIVGLLKALHP+WSPAAIKSAIMTSATISDNTMNL+LDGGSPIFAPATPFIYG
Sbjct: 556 MSCPHVSGIVGLLKALHPQWSPAAIKSAIMTSATISDNTMNLILDGGSPIFAPATPFIYG 615
Query: 623 SGHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFADENFKCPVSGSILNFNYPS 682
SGHIHP GAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFA+EN+KCPVSGSILNFNYPS
Sbjct: 616 SGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFAEENYKCPVSGSILNFNYPS 675
Query: 683 IGVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMT 742
IGVQNLTG VT+TRRLKNVGRPGVYRVRVRRP+GVKV VKPRVLKF KIGEEKRFELTMT
Sbjct: 676 IGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVLVKPRVLKFRKIGEEKRFELTMT 735
Query: 743 GAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 751
G VAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF
Sbjct: 736 GVVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 768
BLAST of CmaCh13G003330 vs. NCBI nr
Match:
XP_022927102.1 (subtilisin-like protease SBT5.4 [Cucurbita moschata])
HSP 1 Score: 1379.0 bits (3568), Expect = 0.0e+00
Identity = 683/752 (90.82%), Postives = 706/752 (93.88%), Query Frame = 0
Query: 24 KKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAA 83
K+SYIVLLGSHSHGLEVS +DLQRVVDSHHKLLGS LGSSEKAKDAIF+SYKNHINGFAA
Sbjct: 251 KESYIVLLGSHSHGLEVSAVDLQRVVDSHHKLLGSLLGSSEKAKDAIFHSYKNHINGFAA 310
Query: 84 ILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFILPFSPWRKARFGKDVI 143
IL+EEEATK AKHPEVAAVLPNKAKELHTTHSWEFMHLEKNG +LPFSPWRKARFG+DVI
Sbjct: 311 ILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGVVLPFSPWRKARFGRDVI 370
Query: 144 IANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTPDAVPCNRKLIGAKYFNQGVIAYL 203
IANLDTGVWPESKSFGEQGIVGGVPSRWKGGC DKTPDAVPCNRKLIGAKYFNQG+IAYL
Sbjct: 371 IANLDTGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDAVPCNRKLIGAKYFNQGLIAYL 430
Query: 204 KSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSGVSVFGSGIGTAKGGSPKARVAAY 263
KS NLTDEL LIVNSTRDYVGHG+HTLSTAGG+YVSGVSVFGSGIGTAKGGSPKARVAAY
Sbjct: 431 KSHNLTDELSLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAY 490
Query: 264 KVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIGSPPAEYYDDTIAIASFHALKKGI 323
KVCWPFHNSGGCYD D+ DG DQAI+DGVDVLSLSIGSPPAEYYDDTIAIASF A+KKGI
Sbjct: 491 KVCWPFHNSGGCYDIDVVDGFDQAIHDGVDVLSLSIGSPPAEYYDDTIAIASFLAIKKGI 550
Query: 324 PVVCSAGNSGPSMATATNIAPWILTVGASTLD-------------------------RRK 383
PVVCSAGNSGPSMA+A+NIAPWILTVGASTLD RRK
Sbjct: 551 PVVCSAGNSGPSMASASNIAPWILTVGASTLDREFQAPIELGNGQRFWGSSLSRPLARRK 610
Query: 384 LYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKILVCLTGGSSRIDKGMQAVLAGAV 443
LYPLITGAQAKATTASADDAMLCKPKTLD SKVNGKILVCLTGGSSRIDKGMQAVLAGAV
Sbjct: 611 LYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAV 670
Query: 444 GMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLIPPSSKVNTKPS 503
GMILCNDRFSGFKIIADLHVLPASHI+YNDGQAV SYINS KNPMGYLIPPSSKVNTKPS
Sbjct: 671 GMILCNDRFSGFKIIADLHVLPASHINYNDGQAVFSYINSWKNPMGYLIPPSSKVNTKPS 730
Query: 504 PIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGAVSPTGEPFDNRTVPYITMSGTSM 563
P MAAFSSRGPNM+SPEIIKPDVTAPGVNIIAAFSGAVSPT EPFDNRTVPYITMSGTSM
Sbjct: 731 PTMAAFSSRGPNMVSPEIIKPDVTAPGVNIIAAFSGAVSPTREPFDNRTVPYITMSGTSM 790
Query: 564 SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLMLDGGSPIFAPATPFIYGS 623
SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSA ISDNTMNL+LDGGSP+FAPATPFIYGS
Sbjct: 791 SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSAIISDNTMNLILDGGSPMFAPATPFIYGS 850
Query: 624 GHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFADENFKCPVSGSILNFNYPSI 683
GHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFADENFKCP+S SI NFNYPSI
Sbjct: 851 GHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFADENFKCPISCSIFNFNYPSI 910
Query: 684 GVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTMTG 743
GVQNLTGSVT+ RRLKNVGRPGVYRVRVR+PEGVKVSVKPRVLKFWKIGEEKRFELTMTG
Sbjct: 911 GVQNLTGSVTLKRRLKNVGRPGVYRVRVRQPEGVKVSVKPRVLKFWKIGEEKRFELTMTG 970
Query: 744 AVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 751
A+AEGQIGYGTLIWTDGKHFV+SPIVVSSG F
Sbjct: 971 AMAEGQIGYGTLIWTDGKHFVRSPIVVSSGLF 1002
BLAST of CmaCh13G003330 vs. NCBI nr
Match:
XP_023520266.1 (subtilisin-like protease SBT5.4 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1333.9 bits (3451), Expect = 0.0e+00
Identity = 665/775 (85.81%), Postives = 697/775 (89.94%), Query Frame = 0
Query: 1 MNFTNFILILFWFFRLISPVIAVKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFL 60
MNF F+LILF FF LISP IA+KKSYIVLLGSH+HGLEVS +DLQR VDSHHKLLGSFL
Sbjct: 1 MNFPTFVLILFGFFWLISPAIAIKKSYIVLLGSHAHGLEVSAVDLQRAVDSHHKLLGSFL 60
Query: 61 GSSEKAKDAIFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMH 120
GSS KAKDAIFYSYKNHINGFAAILDEEEATK AKHPEVAAVLPNKAKELHTT SWEFMH
Sbjct: 61 GSSGKAKDAIFYSYKNHINGFAAILDEEEATKLAKHPEVAAVLPNKAKELHTTRSWEFMH 120
Query: 121 LEKNGFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTP 180
LEKNG P SPWRKA+ G++VIIANLDTGVWPESKSFGE GIVGGVPSRWKGGCTDKTP
Sbjct: 121 LEKNGVSPPSSPWRKAKSGRNVIIANLDTGVWPESKSFGEHGIVGGVPSRWKGGCTDKTP 180
Query: 181 DAVPCNRKLIGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSG 240
DAVPCNRKLIGAKYFN GVIAYLKS+NLT EL IVNSTRDY GHGSHTLSTAGG+YVSG
Sbjct: 181 DAVPCNRKLIGAKYFNHGVIAYLKSENLTKELSFIVNSTRDYEGHGSHTLSTAGGSYVSG 240
Query: 241 VSVFGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIG 300
VSVFGSGIGTAKGGSPKA VAAYKVCWP H++GGC+DADI D AI+DGVDVLSLS+G
Sbjct: 241 VSVFGSGIGTAKGGSPKAHVAAYKVCWPLHDTGGCFDADIAKAFDHAIHDGVDVLSLSLG 300
Query: 301 SPPAEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDR---- 360
SPPAEYYDD IAIASFHALKKGIPVVCSAGN GPSMAT +N APWILTVGASTLDR
Sbjct: 301 SPPAEYYDDIIAIASFHALKKGIPVVCSAGNDGPSMATVSNTAPWILTVGASTLDREFQA 360
Query: 361 ---------------------RKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKI 420
RKLYPLITGAQAKATTAS DDAMLCKPKTLD SKV GKI
Sbjct: 361 PIELRNGKHFKGSSLSGPLSGRKLYPLITGAQAKATTASTDDAMLCKPKTLDHSKVKGKI 420
Query: 421 LVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSY 480
LVCL G SSR+DKG+QA+LAGAVGMILCNDR SGF+IIAD HVLPASHISYNDGQ VSSY
Sbjct: 421 LVCLRGDSSRVDKGVQALLAGAVGMILCNDRLSGFEIIADPHVLPASHISYNDGQVVSSY 480
Query: 481 INSRKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGA 540
INS KNPMG+LIPP SKVNTKPSP MAAFSSRGPNM+SPEIIKPDVTAPGVNIIAAFSGA
Sbjct: 481 INSTKNPMGHLIPPLSKVNTKPSPTMAAFSSRGPNMVSPEIIKPDVTAPGVNIIAAFSGA 540
Query: 541 VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDN 600
VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHP+WSPAAIKSAIMTSA I DN
Sbjct: 541 VSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPKWSPAAIKSAIMTSARIRDN 600
Query: 601 TMNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIR 660
TMN+MLDGGSPIFAPATPFI+GSGHI PTGAIDPGLVYDLSPNDYLEFLCASGYKEKN+R
Sbjct: 601 TMNIMLDGGSPIFAPATPFIFGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNLR 660
Query: 661 VFADENFKCPVSGSILNFNYPSIGVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVS 720
VFAD NFKCP+S SILNFNYPSIGVQNLTG+VT+TRRLKNVGRPGVYRVRV++PEGVKVS
Sbjct: 661 VFADGNFKCPISSSILNFNYPSIGVQNLTGNVTLTRRLKNVGRPGVYRVRVQQPEGVKVS 720
Query: 721 VKPRVLKFWKIGEEKRFELTMTGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 751
VKP VLKF KIGEEKRFELTM GA+A QIGYGTLIWTDGKHFV+SPIVVSSGFF
Sbjct: 721 VKPSVLKFKKIGEEKRFELTMIGAMANSQIGYGTLIWTDGKHFVRSPIVVSSGFF 775
BLAST of CmaCh13G003330 vs. TAIR 10
Match:
AT5G59810.1 (Subtilase family protein )
HSP 1 Score: 829.7 bits (2142), Expect = 1.9e-240
Identity = 427/771 (55.38%), Postives = 528/771 (68.48%), Query Frame = 0
Query: 2 NFTNFILILFWFFRLISPVIAVKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFLG 61
+ ++ +L++ FF SP A+KKSYIV LGSH+H ++S+ L V SH L SF+G
Sbjct: 20 SLSSLLLLVTLFF---SPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVG 79
Query: 62 SSEKAKDAIFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMHL 121
S E AK+AIFYSYK HINGFAAILDE EA + AKHP+V +V PNK ++LHTTHSW FM L
Sbjct: 80 SHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLL 139
Query: 122 EKNGFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTPD 181
KNG + S W KA +G+D IIANLDTGVWPESKSF ++G G VP+RWKG C
Sbjct: 140 AKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEG-YGAVPARWKGRCHKD--- 199
Query: 182 AVPCNRKLIGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSGV 241
VPCNRKLIGA+YFN+G +AY + + RD+ GHGSHTLSTA GN+V G
Sbjct: 200 -VPCNRKLIGARYFNKGYLAYTGLPSNAS-----YETCRDHDGHGSHTLSTAAGNFVPGA 259
Query: 242 SVFGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIGS 301
+VFG G GTA GGSPKARVAAYKVCWP + C+DADI I+ AI DGVDVLS S+G
Sbjct: 260 NVFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGG 319
Query: 302 PPAEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDR----- 361
+Y D IAI SFHA+K G+ VVCSAGNSGP T +N+APW++TVGAS++DR
Sbjct: 320 DAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAF 379
Query: 362 --------------------RKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKIL 421
K+Y LI+ A A + DA+LCK +LD KV GKIL
Sbjct: 380 VELKNGQSFKGTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKIL 439
Query: 422 VCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYI 481
VCL G ++R+DKGMQA AGA GM+LCND+ SG +II+D HVLPAS I Y DG+ + SY+
Sbjct: 440 VCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYL 499
Query: 482 NSRKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGAV 541
+S K+P GY+ P++ +NTKP+P MA+FSSRGPN I+P I+KPD+TAPGVNIIAAF+ A
Sbjct: 500 SSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEAT 559
Query: 542 SPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNT 601
PT DNR P+ T SGTSMSCPH+SG+VGLLK LHP WSPAAI+SAIMT++ +N
Sbjct: 560 GPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNR 619
Query: 602 MNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRV 661
M+D F A PF YGSGH+ P A PGLVYDL+ DYL+FLCA GY +++
Sbjct: 620 RKPMVDES---FKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQL 679
Query: 662 FA-DENFKCPVSGSILNFNYPSIGVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVS 721
FA D + C ++L+FNYPSI V NLTGS+TVTR+LKNVG P Y R R P GV+VS
Sbjct: 680 FAEDPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVS 739
Query: 722 VKPRVLKFWKIGEEKRFELTMTG-AVAEGQIGYGTLIWTDGKHFVKSPIVV 746
V+P+ L F K GE K F++T+ V +G L WTD H+V+SPIVV
Sbjct: 740 VEPKQLTFNKTGEVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVV 774
BLAST of CmaCh13G003330 vs. TAIR 10
Match:
AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 771.2 bits (1990), Expect = 7.9e-223
Identity = 399/774 (51.55%), Postives = 526/774 (67.96%), Query Frame = 0
Query: 5 NFILILFWFFRLISPVIAVK--KSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFLGS 64
+F+L+L ++A K SY+V G+HSH E++ + RV ++H+ LGSF GS
Sbjct: 8 SFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGS 67
Query: 65 SEKAKDAIFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMHLE 124
E+A DAIFYSY HINGFAA LD + A + +KHPEV +V PNKA +LHTT SW+F+ LE
Sbjct: 68 RERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLE 127
Query: 125 KNGFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTPDA 184
N ++ S WRKARFG+D IIANLDTGVWPESKSF ++G+ G +PSRWKG C ++
Sbjct: 128 HNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDAT 187
Query: 185 VPCNRKLIGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSGVS 244
CNRKLIGA+YFN+G A + N + + P RD GHGSHTLSTA G++V GVS
Sbjct: 188 FHCNRKLIGARYFNKGYAAAVGHLNSSFDSP------RDLDGHGSHTLSTAAGDFVPGVS 247
Query: 245 VFGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIGSP 304
+FG G GTAKGGSP+ARVAAYKVCWP CYDAD+ D AI+DG DV+S+S+G
Sbjct: 248 IFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGE 307
Query: 305 PAEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDRR----- 364
P +++D++AI SFHA KK I VVCSAGNSGP+ +T +N+APW +TVGAST+DR
Sbjct: 308 PTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNL 367
Query: 365 ---------------------KLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKIL 424
K YP++ AKA ASA DA LCK +LD K GKIL
Sbjct: 368 VLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKIL 427
Query: 425 VCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYI 484
VCL G + R++KG L G +GM+L N +G ++AD HVLPA+ ++ D AVS YI
Sbjct: 428 VCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYI 487
Query: 485 NSRKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGAV 544
+ K P+ ++ P + + KP+P+MA+FSS+GP++++P+I+KPD+TAPGV++IAA++GAV
Sbjct: 488 SQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAV 547
Query: 545 SPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNT 604
SPT E FD R + + +SGTSMSCPH+SGI GLLK +P WSPAAI+SAIMT+ATI D+
Sbjct: 548 SPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDI 607
Query: 605 MNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRV 664
+ + + ATPF +G+GH+ P A++PGLVYDL DYL FLC+ GY I V
Sbjct: 608 PGPIQNATN---MKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISV 667
Query: 665 FADENFKCPVSG-SILNFNYPSIGVQNLTGS-VTVTRRLKNVGRPGVYRVRVRRPEGVKV 724
F+ NF C S++N NYPSI V NLT S VTV+R +KNVGRP +Y V+V P+GV V
Sbjct: 668 FSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYV 727
Query: 725 SVKPRVLKFWKIGEEKRFELTMT---GAVAEGQIGYGTLIWTDGKHFVKSPIVV 746
+VKP L F K+GE+K F++ + G VA+G + +G L+W+D KH V+SPIVV
Sbjct: 728 AVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYV-FGELVWSDKKHRVRSPIVV 770
BLAST of CmaCh13G003330 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 544.3 bits (1401), Expect = 1.6e-154
Identity = 328/781 (42.00%), Postives = 455/781 (58.26%), Query Frame = 0
Query: 1 MNFTNFILILFWFFRLISPVIAVKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFL 60
++ T F L+L F +S + + +YIV ++ + D H S L
Sbjct: 6 LSSTAFFLLLCLGFCHVSSSSSDQGTYIV---------HMAKSQMPSSFDLHSNWYDSSL 65
Query: 61 GSSEKAKDAIFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMH 120
S + + + Y+Y+N I+GF+ L +EEA P V +VLP ELHTT + F+
Sbjct: 66 RSISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLG 125
Query: 121 LEKNGFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTP 180
L+++ L + +A DV++ LDTGVWPESKS+ ++G G +PS WKGGC T
Sbjct: 126 LDEHTADL----FPEAGSYSDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEAGTN 185
Query: 181 -DAVPCNRKLIGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVS 240
A CNRKLIGA++F +G Y + DE S RD GHG+HT STA G+ V
Sbjct: 186 FTASLCNRKLIGARFFARG---YESTMGPIDE-SKESRSPRDDDGHGTHTSSTAAGSVVE 245
Query: 241 GVSVFGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSI 300
G S+ G GTA+G +P+ARVA YKVCW GGC+ +DI ID+AI D V+VLS+S+
Sbjct: 246 GASLLGYASGTARGMAPRARVAVYKVCW----LGGCFSSDILAAIDKAIADNVNVLSMSL 305
Query: 301 GSPPAEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDR--- 360
G ++YY D +AI +F A+++GI V CSAGN+GPS ++ +N+APWI TVGA TLDR
Sbjct: 306 GGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFP 365
Query: 361 -----------------------RKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNG 420
KL P I A ++A + LC TL KV G
Sbjct: 366 ALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNA----SNATNGNLCMTGTLIPEKVKG 425
Query: 421 KILVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVS 480
KI++C G ++R+ KG AG VGMIL N +G +++AD H+LPA+ + G +
Sbjct: 426 KIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIR 485
Query: 481 SYINSRKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFS 540
Y+ + NP + + V KPSP++AAFSSRGPN I+P I+KPD+ APGVNI+AA++
Sbjct: 486 HYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWT 545
Query: 541 GAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSA--T 600
GA PTG D+R V + +SGTSMSCPHVSG+ LLK++HPEWSPAAI+SA+MT+A T
Sbjct: 546 GAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKT 605
Query: 601 ISDNTMNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKE 660
D L + G P+TPF +G+GH+ PT A +PGL+YDL+ DYL FLCA Y
Sbjct: 606 YKDGKPLLDIATGK----PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTS 665
Query: 661 KNIRVFADENFKCPVSG--SILNFNYPSIGVQ-NLTGSVTVTRRLKNVGRPGVYRVRV-R 720
IR + N+ C S S+ + NYPS V + G+ TR + +VG G Y V+V
Sbjct: 666 PQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTS 725
Query: 721 RPEGVKVSVKPRVLKFWKIGEEKRFELTMT--GAVAEGQIGYGTLIWTDGKHFVKSPIVV 747
GVK+SV+P VL F + E+K + +T T + G +G++ W+DGKH V SP+ +
Sbjct: 726 ETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 755
BLAST of CmaCh13G003330 vs. TAIR 10
Match:
AT3G14067.1 (Subtilase family protein )
HSP 1 Score: 533.5 bits (1373), Expect = 2.8e-151
Identity = 330/775 (42.58%), Postives = 446/775 (57.55%), Query Frame = 0
Query: 33 SHSHGLEVSTIDLQR-----VVDSHHKLLGSFLGS--SEKAKDAIFYSYKNHINGFAAIL 92
S S GLE + +QR + SH+ S L S S + YSY ++GF+A L
Sbjct: 25 SSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATLLYSYSRAVHGFSARL 84
Query: 93 DEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFILPFSPWRKARFGKDVIIA 152
+ +HP V +V+P++A+E+HTTH+ F+ +N + W + +G+DVI+
Sbjct: 85 SPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGL-----WSNSNYGEDVIVG 144
Query: 153 NLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTPD--AVPCNRKLIGAKYFNQGVIAYL 212
LDTG+WPE SF + G+ G +PS WKG C + PD A CNRKLIGA+ F +G YL
Sbjct: 145 VLDTGIWPEHPSFSDSGL-GPIPSTWKGEC-EIGPDFPASSCNRKLIGARAFYRG---YL 204
Query: 213 KSQNLTDE-LPLIVNSTRDYVGHGSHTLSTAGGNYVSGVSVFGSGIGTAKGGSPKARVAA 272
+N T + S RD GHG+HT STA G+ V+ S++ GTA G + KAR+AA
Sbjct: 205 TQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAA 264
Query: 273 YKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIGSPPA--EYYDDTIAIASFHALK 332
YK+CW +GGCYD+DI +DQA+ DGV V+SLS+G+ + EY+ D+IAI +F A +
Sbjct: 265 YKICW----TGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATR 324
Query: 333 KGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDRRKLYPLITG--------------- 392
GI V CSAGNSGP+ TATNIAPWILTVGAST+DR ITG
Sbjct: 325 HGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGES 384
Query: 393 ---AQAKATTASADDAMLCKPKTLDRSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGMIL 452
+Q + + LC P L+ S V GKI++C GG++R++KG LAG GMIL
Sbjct: 385 LPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGMIL 444
Query: 453 CNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNP------MGYLIPPSSKVNTK 512
N SG ++ AD H++PA+ + G + YI + +P +G LI PS
Sbjct: 445 ANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPS-----P 504
Query: 513 PSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGAVSPTGEPFDNRTVPYITMSGT 572
PSP +AAFSSRGPN ++P I+KPDV APGVNI+A ++G V PT D R V + +SGT
Sbjct: 505 PSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGT 564
Query: 573 SMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLMLDGGSPIFAPAT---- 632
SMSCPHVSG+ LL+ HP+WSPAAIKSA++T+A +N+ G PI AT
Sbjct: 565 SMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENS-------GEPIEDLATGKSS 624
Query: 633 -PFIYGSGHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVF--------ADENFK 692
FI+G+GH+ P A++PGLVYD+ +Y+ FLCA GY+ I VF A E K
Sbjct: 625 NSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSK 684
Query: 693 CPVSGSILNFNYPSIGVQ-NLTGSVTVTRR-LKNVGR--PGVYRVRVRRPEGVKVSVKPR 749
+G + NYPS V TG V +R +KNVG VY V V+ P V++ V P
Sbjct: 685 LRTAGDL---NYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPS 744
BLAST of CmaCh13G003330 vs. TAIR 10
Match:
AT4G34980.1 (subtilisin-like serine protease 2 )
HSP 1 Score: 515.0 bits (1325), Expect = 1.0e-145
Identity = 304/731 (41.59%), Postives = 428/731 (58.55%), Query Frame = 0
Query: 62 SSEKAKDA-IFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMH 121
S+E A+++ I + Y +GF+A++ +EA HP V AV ++ +ELHTT S +F+
Sbjct: 49 STEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLG 108
Query: 122 LEKNGFILPFSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTP 181
L+ + W ++ +G DVII DTG+WPE +SF + + G +P RW+G C
Sbjct: 109 LQNQKGL-----WSESDYGSDVIIGVFDTGIWPERRSFSDLNL-GPIPKRWRGVCESGAR 168
Query: 182 -DAVPCNRKLIGAKYFNQG-VIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYV 241
CNRK+IGA++F +G A + N T E S RD GHG+HT STA G +
Sbjct: 169 FSPRNCNRKIIGARFFAKGQQAAVIGGINKTVEF----LSPRDADGHGTHTSSTAAGRHA 228
Query: 242 SGVSVFGSGIGTAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLS 301
S+ G G AKG +PKAR+AAYKVCW GC D+DI D A+ DGVDV+S+S
Sbjct: 229 FKASMSGYASGVAKGVAPKARIAAYKVCW---KDSGCLDSDILAAFDAAVRDGVDVISIS 288
Query: 302 IGSP---PAEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLD 361
IG + YY D IAI S+ A KGI V SAGN GP+ + TN+APW+ TVGAST+D
Sbjct: 289 IGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTID 348
Query: 362 RR--------------------------KLYPLITGAQAKATTASADDAMLCKPKTLDRS 421
R +++P++ ++ ++AS LC TLD
Sbjct: 349 RNFPADAILGDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCMENTLDPK 408
Query: 422 KVNGKILVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDG 481
+V GKI++C G S R+ KG+ AG VGMIL N +G ++ D H++PA + N+G
Sbjct: 409 QVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEG 468
Query: 482 QAVSSYINSRKNPMGYLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNII 541
+ +Y +S NP+ + + V KP+P++A+FS RGPN +SPEI+KPD+ APGVNI+
Sbjct: 469 DRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNIL 528
Query: 542 AAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTS 601
AA++ AV PTG P D R + +SGTSM+CPHVSG LLK+ HP+WSPA I+SA+MT+
Sbjct: 529 AAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTT 588
Query: 602 ATISDNTMNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGLVYDLSPNDYLEFLCASGY 661
+ DN+ ++D + ATP+ YGSGH++ A++PGLVYD++ +DY+ FLC+ GY
Sbjct: 589 TNLVDNSNRSLIDESTG--KSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGY 648
Query: 662 KEKNIRVFADENFKCPVS--GSILNFNYPSIGV---QNLTG--SVTVTRRLKNVGR-PGV 721
K I+V +CP + S N NYPSI N G S TV R NVG+ V
Sbjct: 649 GPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAV 708
Query: 722 YRVRVRRPEGVKVSVKPRVLKFWKIGEEKRFELTM---TGAVAEGQIG--YGTLIWTD-G 747
YR R+ P GV V+VKP L F + + + +T+ T V G+ G +G++ W D G
Sbjct: 709 YRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGG 759
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| F4JXC5 | 2.6e-239 | 55.38 | Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... | [more] |
| Q9ZSP5 | 1.1e-221 | 51.55 | Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... | [more] |
| I1N462 | 3.1e-184 | 47.51 | Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... | [more] |
| O65351 | 2.2e-153 | 42.00 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
| A9JQS7 | 1.3e-150 | 41.73 | Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1I7Q4 | 0.0e+00 | 92.65 | subtilisin-like protease SBT5.4 OS=Cucurbita maxima OX=3661 GN=LOC111471617 PE=3... | [more] |
| A0A6J1EK27 | 0.0e+00 | 91.63 | subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434038 PE... | [more] |
| A0A6J1EG71 | 0.0e+00 | 90.82 | subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434037 PE... | [more] |
| A0A6J1EG66 | 0.0e+00 | 86.40 | subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434032 PE... | [more] |
| A0A6J1EGQ8 | 0.0e+00 | 85.39 | subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434031 PE... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| KAG6583696.1 | 0.0e+00 | 91.23 | Subtilisin-like protease 5.4, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| XP_022973086.1 | 0.0e+00 | 92.65 | subtilisin-like protease SBT5.4 [Cucurbita maxima] | [more] |
| XP_022927103.1 | 0.0e+00 | 91.63 | subtilisin-like protease SBT5.4 [Cucurbita moschata] | [more] |
| XP_022927102.1 | 0.0e+00 | 90.82 | subtilisin-like protease SBT5.4 [Cucurbita moschata] | [more] |
| XP_023520266.1 | 0.0e+00 | 85.81 | subtilisin-like protease SBT5.4 [Cucurbita pepo subsp. pepo] | [more] |
