CmaCh13G003120 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh13G003120
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionexopolygalacturonase-like
LocationCma_Chr13: 3423649 .. 3425066 (+)
RNA-Seq ExpressionCmaCh13G003120
SyntenyCmaCh13G003120
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AATGCATGGAAGGTTGCATGCACGTCCAATCGTTCTAGTGCGATTGTTATTCCGGGAGGGATATATAAAGTAAAGGAAGGGATATTTAAAGGACCTTGTAATAGCCCAATTGACTTTCAACTTGGAGGAACATTGCAGGCACCAAAAAGTCTTCAGGGAGATAATTGGATTACTTTTGAACATATAGATAGATTGACATTGTCAGGTGAAGGAGTTTTTGATGGCCAAGGAAAGGCAGCTTGGAAAAAGAATGATTTCCATAAACACATAAATTGTGCCCAACTTCCCATTGTAAGCAATATGCCGTCACCTTTCCTTCTCAATCTCGTGCATCTTGTTGTTTTTAGATGTCTTCTCTAATCTTCTAACATTTATAATTTGGTAAACGTTCTTCATTTTCTAGAGGATAATCTTTAACCATAATAAAAAATAAATAAAAAATCATCTAAAGGAGTGAAATTCTTTCCGAGTTTTCGATTTTTTTGGGTTTCGTTAAAATTTTTGGCTTCATTACTGATAGCAAGAACTTCATTCGGTTAAATTCTTTCTCACAAAATATTATTTGATTATACAAAATGTTTAGAGTTTGAGGTTCAACTTCATCACCAATTCAATCGTGAAGAGAATAACGTCACTTGATAGCAAGAACTTCCATATCAACGTCCTCGGCTGCAACAACCTCACATTCCATAATATCAATATAACTGCACCAGAAAACAGTCCCAACACCGATGGAATACACATCGGTCGATCAAATAGTTTCGCAATCACAATGTCCAAAATTGCAACCGGAGATGATTGAATTTCTCTTGGAGATGGAAGCAAACAAATTAACATCACCAACGTGACATGTGGGCCAGGACATGGCATAAGCATCGGAAGTCTTGGAAGATACACTAACGAAGAACCCGTTGAAGGTGTCATCGTAAACAACTGCACGATAATTAACACCACTAATGGTGTCAGGATCAAAACGTGGCCAGCTTCTGCTGCTAGCGGCACCGCCACCAATATGCAGTTTTTGGACATTATAATGGTCAATGTTAGCAACCCTATTCTAATAGACCAAGAGTATTGTCCGTGGAATCAGTGCAATCGGAAGGTATTGATCGCATTCTCAAAGCTTTGATTGATTTTGTCACTATAACTTTCACAAATAAACCACTTACAGAAATTTGTTGTCTCATTTTTATTTTCAGATTCCTTCGAAAATTAAGATTAGTAAAGTGAGCTTCAAGAACATTAGAGAAAGTTGTGCAACTCCAGTGGCAATCAAACTTATTTGCAGCCGTCACTTACCATGTGACGAAGTAAAAGTCGGTGACATTGACCTCACTTACAATGGAATAAATGGTCCTATTACATCTCAGTGCACGAATGTGAGACCCATCATTTCAGGAAAACAGAACCCTAAAATC

mRNA sequence

AATGCATGGAAGGTTGCATGCACGTCCAATCGTTCTAGTGCGATTGTTATTCCGGGAGGGATATATAAAGTAAAGGAAGGGATATTTAAAGGACCTTGTAATAGCCCAATTGACTTTCAACTTGGAGGAACATTGCAGGCACCAAAAAGTCTTCAGGGAGATAATTGGATTACTTTTGAACATATAGATAGATTGACATTGTCAGGTGAAGGAGTTTTTGATGGCCAAGGAAAGGCAGCTTGGAAAAAGAATGATTTCCATAAACACATAAATTGTGCCCAACTTCCCATTAGTTTGAGGTTCAACTTCATCACCAATTCAATCGTGAAGAGAATAACGTCACTTGATAGCAAGAACTTCCATATCAACGTCCTCGGCTGCAACAACCTCACATTCCATAATATCAATATAACTGCACCAGAAAACAGTCCCAACACCGATGGAATACACATCGGTCGATCAAATAGTTTCGCAATCACAATCAAACAAATTAACATCACCAACGTGACATGTGGGCCAGGACATGGCATAAGCATCGGAAGTCTTGGAAGATACACTAACGAAGAACCCGTTGAAGGTGTCATCGTAAACAACTGCACGATAATTAACACCACTAATGGTGTCAGGATCAAAACGTGGCCAGCTTCTGCTGCTAGCGGCACCGCCACCAATATGCAGTTTTTGGACATTATAATGGTCAATGTTAGCAACCCTATTCTAATAGACCAAGAGTATTGTCCGTGGAATCAGTGCAATCGGAAGATTCCTTCGAAAATTAAGATTAGTAAAGTGAGCTTCAAGAACATTAGAGAAAGTTGTGCAACTCCAGTGGCAATCAAACTTATTTGCAGCCGTCACTTACCATGTGACGAAGTAAAAGTCGGTGACATTGACCTCACTTACAATGGAATAAATGGTCCTATTACATCTCAGTGCACGAATGTGAGACCCATCATTTCAGGAAAACAGAACCCTAAAATC

Coding sequence (CDS)

AATGCATGGAAGGTTGCATGCACGTCCAATCGTTCTAGTGCGATTGTTATTCCGGGAGGGATATATAAAGTAAAGGAAGGGATATTTAAAGGACCTTGTAATAGCCCAATTGACTTTCAACTTGGAGGAACATTGCAGGCACCAAAAAGTCTTCAGGGAGATAATTGGATTACTTTTGAACATATAGATAGATTGACATTGTCAGGTGAAGGAGTTTTTGATGGCCAAGGAAAGGCAGCTTGGAAAAAGAATGATTTCCATAAACACATAAATTGTGCCCAACTTCCCATTAGTTTGAGGTTCAACTTCATCACCAATTCAATCGTGAAGAGAATAACGTCACTTGATAGCAAGAACTTCCATATCAACGTCCTCGGCTGCAACAACCTCACATTCCATAATATCAATATAACTGCACCAGAAAACAGTCCCAACACCGATGGAATACACATCGGTCGATCAAATAGTTTCGCAATCACAATCAAACAAATTAACATCACCAACGTGACATGTGGGCCAGGACATGGCATAAGCATCGGAAGTCTTGGAAGATACACTAACGAAGAACCCGTTGAAGGTGTCATCGTAAACAACTGCACGATAATTAACACCACTAATGGTGTCAGGATCAAAACGTGGCCAGCTTCTGCTGCTAGCGGCACCGCCACCAATATGCAGTTTTTGGACATTATAATGGTCAATGTTAGCAACCCTATTCTAATAGACCAAGAGTATTGTCCGTGGAATCAGTGCAATCGGAAGATTCCTTCGAAAATTAAGATTAGTAAAGTGAGCTTCAAGAACATTAGAGAAAGTTGTGCAACTCCAGTGGCAATCAAACTTATTTGCAGCCGTCACTTACCATGTGACGAAGTAAAAGTCGGTGACATTGACCTCACTTACAATGGAATAAATGGTCCTATTACATCTCAGTGCACGAATGTGAGACCCATCATTTCAGGAAAACAGAACCCTAAAATC

Protein sequence

NAWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFEHIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDSKNFHINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNSFAITIKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKLICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI
Homology
BLAST of CmaCh13G003120 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 4.7e-100
Identity = 184/346 (53.18%), Postives = 229/346 (66.18%), Query Frame = 0

Query: 2   AWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQA---PKSLQGDNWIT 61
           AWK AC S   S I++P G + V     +GPC S I  QL GTL+A   P  ++G  WI 
Sbjct: 9   AWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSKIKGLGWIN 68

Query: 62  FEHIDRLTLSGEGVFDGQGKAAWKKNDFHKH-INCAQLPISLRFNFITNSIVKRITSLDS 121
              ID LT+ G GVFDGQGK+AW +ND HK+   C  L ++LR   +TNSI++ +T+LDS
Sbjct: 69  LNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSILRDVTTLDS 128

Query: 122 KNFHINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNSFAIT----------------I 181
           KNFH+NV+GC NLTF    I+A E S NTDGIHIGRS+   I                  
Sbjct: 129 KNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDDCISLGDGS 188

Query: 182 KQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGT 241
           K INITN+TCGPGHGIS+GSLGRY NEE V G+ V NCTI  + NGVRIKTWP S   G 
Sbjct: 189 KNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTWPKS-EPGE 248

Query: 242 ATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKL 301
           A+ M F DI M +V  PILIDQ YCP+NQC  ++PS +K+SK+SFKNI+ +  T  A+KL
Sbjct: 249 ASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTSTTKEAVKL 308

Query: 302 ICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 328
           +CS+  PC+ V++ DIDLTY+G  GP TS C N++P I GKQ P I
Sbjct: 309 VCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQIPAI 353

BLAST of CmaCh13G003120 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 3.1e-96
Identity = 173/346 (50.00%), Postives = 232/346 (67.05%), Query Frame = 0

Query: 1   NAWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQA---PKSLQGDNWI 60
           NA+K AC S   S IVIP G + + +   +GPC SP++ Q+  TL+A   P  L+   W+
Sbjct: 46  NAFKEACQSTSPSTIVIPKGTFTMNQVKLEGPCKSPLELQIQATLKAPSDPSQLKVGEWL 105

Query: 61  TFEHIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDS 120
           T   +D+ T+SG G+ DGQ  AAW+     K   C +LP +L FN +TNS +K IT+LDS
Sbjct: 106 TVNKLDQFTMSGGGILDGQAAAAWECKQSKK---CNKLPNNLSFNSLTNSTIKDITTLDS 165

Query: 121 KNFHINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNSFAIT----------------I 180
           K+FH+NV  C NLTF   N++AP NSPNTDGIH+ RS+S  IT                 
Sbjct: 166 KSFHVNVNQCKNLTFIRFNVSAPANSPNTDGIHVSRSSSVNITDSNFSTGDDCISVGDET 225

Query: 181 KQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGT 240
           +Q+ IT VTCGPGHGIS+GSLG   +E+PV GV V NCT  NT NGVRIKTWPAS   G 
Sbjct: 226 EQLYITRVTCGPGHGISVGSLGGNPDEKPVVGVFVRNCTFTNTDNGVRIKTWPAS-HPGV 285

Query: 241 ATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKL 300
             ++ F DII+ NVSNP++IDQ YCP+N+CN+ +PS++KISKVSF+NI+ +  T  A+ L
Sbjct: 286 VNDVHFEDIIVQNVSNPVVIDQVYCPFNKCNKDLPSQVKISKVSFQNIKGTSRTQDAVSL 345

Query: 301 ICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 328
           + S+ +PC+ ++VGDID+TY+G  GP  S C N++P + GKQNP +
Sbjct: 346 LRSKGVPCEGIEVGDIDITYSGKEGPAKSSCENIKPSLKGKQNPPV 387

BLAST of CmaCh13G003120 vs. ExPASy Swiss-Prot
Match: P35337 (Polygalacturonase OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 4.1e-96
Identity = 170/343 (49.56%), Postives = 226/343 (65.89%), Query Frame = 0

Query: 2   AWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAP-KSLQG-DNWITF 61
           A+  AC +   S ++IP G +K+ E +  GPC SPI+F L G ++    S QG D W+ F
Sbjct: 42  AFTSACQAPAPSQVLIPKGDFKLGETVMTGPCKSPIEFTLQGNVKTDGGSTQGKDRWVVF 101

Query: 62  EHIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDSKN 121
           E I+   L+G G FDG+G AAWK N+ HK   C +LPIS+RF+F+ N+ +K +TSLD+KN
Sbjct: 102 EKINGFKLNGGGTFDGEGNAAWKANNCHKTFECKKLPISVRFDFVDNAEIKDVTSLDAKN 161

Query: 122 FHINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNSFAIT----------------IKQ 181
           FH NV+   N+TF NI I AP  SPNTDGIH+GR     I                 +K 
Sbjct: 162 FHFNVISGKNMTFDNIKIIAPAESPNTDGIHLGRCEGVKILNTKIATGDDCISVGDGMKN 221

Query: 182 INITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTAT 241
           + I  V CGPGHGIS+GSLGRY  E+ V  + V NCT+  T+NG+RIKTWP++A + TA 
Sbjct: 222 LLIEKVVCGPGHGISVGSLGRYGWEQDVTDITVKNCTLEGTSNGLRIKTWPSAACTTTAA 281

Query: 242 NMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKLIC 301
            + F DII+  VSNPILIDQEYCPWNQCN+  PS IK+  ++F+NIR +     A+KL+C
Sbjct: 282 GIHFEDIILNKVSNPILIDQEYCPWNQCNKNKPSTIKLVDITFRNIRGTSENKDAVKLLC 341

Query: 302 SRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPK 327
           S+  PC+ V++GDI++ Y G +GP T +CTNV P + G QNPK
Sbjct: 342 SKGHPCENVEIGDINIEYTGPDGPPTFECTNVTPKLVGAQNPK 384

BLAST of CmaCh13G003120 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 7.7e-95
Identity = 167/344 (48.55%), Postives = 223/344 (64.83%), Query Frame = 0

Query: 1   NAWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQA---PKSLQGDNWI 60
           +AWK AC S   S +VIP G Y + +   +GPC +PI+  + GT+QA   P + +  NW+
Sbjct: 51  DAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWV 110

Query: 61  TFEHIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDS 120
            F  ++   + G G+FDGQG  A++KN        ++LP+++RF+F+TN++++ ITS DS
Sbjct: 111 RFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLPVNIRFDFLTNALIQDITSKDS 170

Query: 121 KNFHINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNSFAITIKQIN------------ 180
           K FHINV  C N+T   + I AP+ SPNTDGIH+G+S    I    I             
Sbjct: 171 KLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGT 230

Query: 181 ----ITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGT 240
               I  +TCGPGHGISIGSLG++ NEEPVEG+ ++NCTI NT+NG RIKTWP     G 
Sbjct: 231 KNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGE-HGGA 290

Query: 241 ATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKL 300
            + + F DI M NVS+PILIDQ+YCPWN+C +   SK+K+S +SFKNIR + A P AIK 
Sbjct: 291 VSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKF 350

Query: 301 ICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNP 326
           ICS   PC  V++ DID+ +NG   P TSQC NV+PI SGK NP
Sbjct: 351 ICSGSSPCQNVELADIDIKHNGAE-PATSQCLNVKPITSGKLNP 392

BLAST of CmaCh13G003120 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 1.7e-94
Identity = 165/346 (47.69%), Postives = 225/346 (65.03%), Query Frame = 0

Query: 2   AWKVACTSNRSSAIVIPGGIYKVKEGIFKGPC-NSPIDFQLGGTLQA---PKSLQGDNWI 61
           AWK AC S   S ++IP G Y + E   +GPC  S I FQ+ G ++A   P   + D W+
Sbjct: 30  AWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKGSKIGFQIDGVVKAPADPSKFKSDGWV 89

Query: 62  TFEHIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDS 121
           +F  ID LT+SG G  DGQG+ AW KN+  K+ NC    ++LRF+F+ +++V+ ITSL+S
Sbjct: 90  SFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNCKHAAMNLRFDFLKHAMVRDITSLNS 149

Query: 122 KNFHINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNSFAIT----------------I 181
           K FHINVL C ++TF ++ +TAP  S NTDGIH+G S    IT                 
Sbjct: 150 KMFHINVLECEDITFQHVTVTAPGTSINTDGIHVGISKGVTITNTKIATGDDCISIGPGS 209

Query: 182 KQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGT 241
           + + IT V CGPGHGISIGSLGRY NE+ V G+ V  CT   T NGVR+KTWP ++  G 
Sbjct: 210 QNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITVKGCTFSGTMNGVRVKTWP-NSPPGA 269

Query: 242 ATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKL 301
           AT++ F D+ M NV NP+++DQEYCP+ QC+R+ PS+IK+S ++F NIR +    VA+ +
Sbjct: 270 ATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQAPSRIKLSNINFNNIRGTSTGKVAVVI 329

Query: 302 ICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 328
            CS  +PC  +K+G+I+L+Y G  GP TS C+NV+P  SGKQ P I
Sbjct: 330 ACSHGMPCSNMKIGEINLSYRGAGGPATSTCSNVKPTFSGKQVPAI 374

BLAST of CmaCh13G003120 vs. ExPASy TrEMBL
Match: A0A6J1HYN0 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111469285 PE=3 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 3.7e-185
Identity = 320/343 (93.29%), Postives = 322/343 (93.88%), Query Frame = 0

Query: 1   NAWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE 60
           NAWK ACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE
Sbjct: 53  NAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE 112

Query: 61  HIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDSKNF 120
           HIDRLTLSGEGVFDGQGKAAWKKND HKHINCAQLPISLRFNFITNSIVKRITSLDSKNF
Sbjct: 113 HIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFITNSIVKRITSLDSKNF 172

Query: 121 HINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNSFAITI----------------KQI 180
           HINVLGCNNLTFHN+NITAPENSPNTDGIHIGRSN  AIT+                KQI
Sbjct: 173 HINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQI 232

Query: 181 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 240
           NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN
Sbjct: 233 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 292

Query: 241 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKLICS 300
           MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIR SCATPVAIKLICS
Sbjct: 293 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICS 352

Query: 301 RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 328
           RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI
Sbjct: 353 RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 395

BLAST of CmaCh13G003120 vs. ExPASy TrEMBL
Match: A0A6J1I522 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111469287 PE=3 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 3.7e-185
Identity = 320/343 (93.29%), Postives = 322/343 (93.88%), Query Frame = 0

Query: 1   NAWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE 60
           NAWK ACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE
Sbjct: 53  NAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE 112

Query: 61  HIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDSKNF 120
           HIDRLTLSGEGVFDGQGKAAWKKND HKHINCAQLPISLRFNFITNSIVKRITSLDSKNF
Sbjct: 113 HIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFITNSIVKRITSLDSKNF 172

Query: 121 HINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNSFAITI----------------KQI 180
           HINVLGCNNLTFHN+NITAPENSPNTDGIHIGRSN  AIT+                KQI
Sbjct: 173 HINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQI 232

Query: 181 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 240
           NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN
Sbjct: 233 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 292

Query: 241 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKLICS 300
           MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIR SCATPVAIKLICS
Sbjct: 293 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICS 352

Query: 301 RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 328
           RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI
Sbjct: 353 RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 395

BLAST of CmaCh13G003120 vs. ExPASy TrEMBL
Match: A0A6J1I2E9 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111469289 PE=3 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 1.6e-183
Identity = 319/343 (93.00%), Postives = 321/343 (93.59%), Query Frame = 0

Query: 1   NAWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE 60
           NAWK ACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE
Sbjct: 53  NAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE 112

Query: 61  HIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDSKNF 120
           HIDRLTLSGEGVFDGQGKAA KKND HKHINCAQLPISLRFNFITNSIVKRITSLDSKNF
Sbjct: 113 HIDRLTLSGEGVFDGQGKAASKKNDCHKHINCAQLPISLRFNFITNSIVKRITSLDSKNF 172

Query: 121 HINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNSFAITI----------------KQI 180
           HINVLGCNNLTFHN+NITAPENSPNTDGIHIGRSN  AIT+                KQI
Sbjct: 173 HINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQI 232

Query: 181 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 240
           NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN
Sbjct: 233 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 292

Query: 241 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKLICS 300
           MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIR SCATPVAIKLICS
Sbjct: 293 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICS 352

Query: 301 RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 328
           RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI
Sbjct: 353 RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 395

BLAST of CmaCh13G003120 vs. ExPASy TrEMBL
Match: A0A6J1EHA1 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434295 PE=3 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 1.1e-181
Identity = 316/343 (92.13%), Postives = 319/343 (93.00%), Query Frame = 0

Query: 1   NAWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE 60
           NAWK ACTSNRSSAIVIPGGI+KVKEGIFKGPCNSPIDFQLGGTLQAPKSLQG+NWITFE
Sbjct: 53  NAWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGNNWITFE 112

Query: 61  HIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDSKNF 120
            IDRLTLSGEGVFDGQGKAAWKKND HK INCAQLPISLRFNFITNSIVKRITSLDSKNF
Sbjct: 113 RIDRLTLSGEGVFDGQGKAAWKKNDCHKRINCAQLPISLRFNFITNSIVKRITSLDSKNF 172

Query: 121 HINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNSFAITI----------------KQI 180
           HINVLGCNNLTFHNINITAPENSPNTDGIHIGRSN  AIT+                KQI
Sbjct: 173 HINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQI 232

Query: 181 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 240
           NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN
Sbjct: 233 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 292

Query: 241 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKLICS 300
           MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIR SCATPVAIKLICS
Sbjct: 293 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICS 352

Query: 301 RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 328
           RHLPCDEVKVGDIDLTYNGI GPITSQCTNVRPIISGKQNPKI
Sbjct: 353 RHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNPKI 395

BLAST of CmaCh13G003120 vs. ExPASy TrEMBL
Match: A0A6J1F4C6 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111440472 PE=3 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 1.1e-181
Identity = 316/343 (92.13%), Postives = 319/343 (93.00%), Query Frame = 0

Query: 1   NAWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE 60
           NAWK ACTSNRSSAIVIPGGI+KVKEGIFKGPCNSPIDFQLGGTLQAPKSLQG+NWITFE
Sbjct: 53  NAWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGNNWITFE 112

Query: 61  HIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDSKNF 120
            IDRLTLSGEGVFDGQGKAAWKKND HK INCAQLPISLRFNFITNSIVKRITSLDSKNF
Sbjct: 113 RIDRLTLSGEGVFDGQGKAAWKKNDCHKRINCAQLPISLRFNFITNSIVKRITSLDSKNF 172

Query: 121 HINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNSFAITI----------------KQI 180
           HINVLGCNNLTFHNINITAPENSPNTDGIHIGRSN  AIT+                KQI
Sbjct: 173 HINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQI 232

Query: 181 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 240
           NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN
Sbjct: 233 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 292

Query: 241 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKLICS 300
           MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIR SCATPVAIKLICS
Sbjct: 293 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICS 352

Query: 301 RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 328
           RHLPCDEVKVGDIDLTYNGI GPITSQCTNVRPIISGKQNPKI
Sbjct: 353 RHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNPKI 395

BLAST of CmaCh13G003120 vs. NCBI nr
Match: XP_022970272.1 (exopolygalacturonase-like [Cucurbita maxima] >XP_022970273.1 exopolygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 657.1 bits (1694), Expect = 7.7e-185
Identity = 320/343 (93.29%), Postives = 322/343 (93.88%), Query Frame = 0

Query: 1   NAWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE 60
           NAWK ACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE
Sbjct: 53  NAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE 112

Query: 61  HIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDSKNF 120
           HIDRLTLSGEGVFDGQGKAAWKKND HKHINCAQLPISLRFNFITNSIVKRITSLDSKNF
Sbjct: 113 HIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFITNSIVKRITSLDSKNF 172

Query: 121 HINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNSFAITI----------------KQI 180
           HINVLGCNNLTFHN+NITAPENSPNTDGIHIGRSN  AIT+                KQI
Sbjct: 173 HINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQI 232

Query: 181 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 240
           NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN
Sbjct: 233 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 292

Query: 241 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKLICS 300
           MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIR SCATPVAIKLICS
Sbjct: 293 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICS 352

Query: 301 RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 328
           RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI
Sbjct: 353 RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 395

BLAST of CmaCh13G003120 vs. NCBI nr
Match: XP_022970274.1 (exopolygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 657.1 bits (1694), Expect = 7.7e-185
Identity = 320/343 (93.29%), Postives = 322/343 (93.88%), Query Frame = 0

Query: 1   NAWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE 60
           NAWK ACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE
Sbjct: 53  NAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE 112

Query: 61  HIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDSKNF 120
           HIDRLTLSGEGVFDGQGKAAWKKND HKHINCAQLPISLRFNFITNSIVKRITSLDSKNF
Sbjct: 113 HIDRLTLSGEGVFDGQGKAAWKKNDCHKHINCAQLPISLRFNFITNSIVKRITSLDSKNF 172

Query: 121 HINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNSFAITI----------------KQI 180
           HINVLGCNNLTFHN+NITAPENSPNTDGIHIGRSN  AIT+                KQI
Sbjct: 173 HINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQI 232

Query: 181 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 240
           NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN
Sbjct: 233 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 292

Query: 241 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKLICS 300
           MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIR SCATPVAIKLICS
Sbjct: 293 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICS 352

Query: 301 RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 328
           RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI
Sbjct: 353 RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 395

BLAST of CmaCh13G003120 vs. NCBI nr
Match: XP_022970275.1 (exopolygalacturonase-like [Cucurbita maxima])

HSP 1 Score: 651.7 bits (1680), Expect = 3.2e-183
Identity = 319/343 (93.00%), Postives = 321/343 (93.59%), Query Frame = 0

Query: 1   NAWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE 60
           NAWK ACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE
Sbjct: 53  NAWKDACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE 112

Query: 61  HIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDSKNF 120
           HIDRLTLSGEGVFDGQGKAA KKND HKHINCAQLPISLRFNFITNSIVKRITSLDSKNF
Sbjct: 113 HIDRLTLSGEGVFDGQGKAASKKNDCHKHINCAQLPISLRFNFITNSIVKRITSLDSKNF 172

Query: 121 HINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNSFAITI----------------KQI 180
           HINVLGCNNLTFHN+NITAPENSPNTDGIHIGRSN  AIT+                KQI
Sbjct: 173 HINVLGCNNLTFHNVNITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQI 232

Query: 181 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 240
           NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN
Sbjct: 233 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 292

Query: 241 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKLICS 300
           MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIR SCATPVAIKLICS
Sbjct: 293 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICS 352

Query: 301 RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 328
           RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI
Sbjct: 353 RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 395

BLAST of CmaCh13G003120 vs. NCBI nr
Match: XP_022927477.1 (exopolygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 645.6 bits (1664), Expect = 2.3e-181
Identity = 316/343 (92.13%), Postives = 319/343 (93.00%), Query Frame = 0

Query: 1   NAWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE 60
           NAWK ACTSNRSSAIVIPGGI+KVKEGIFKGPCNSPIDFQLGGTLQAPKSLQG+NWITFE
Sbjct: 53  NAWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGNNWITFE 112

Query: 61  HIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDSKNF 120
            IDRLTLSGEGVFDGQGKAAWKKND HK INCAQLPISLRFNFITNSIVKRITSLDSKNF
Sbjct: 113 RIDRLTLSGEGVFDGQGKAAWKKNDCHKRINCAQLPISLRFNFITNSIVKRITSLDSKNF 172

Query: 121 HINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNSFAITI----------------KQI 180
           HINVLGCNNLTFHNINITAPENSPNTDGIHIGRSN  AIT+                KQI
Sbjct: 173 HINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQI 232

Query: 181 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 240
           NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN
Sbjct: 233 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 292

Query: 241 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKLICS 300
           MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIR SCATPVAIKLICS
Sbjct: 293 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICS 352

Query: 301 RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 328
           RHLPCDEVKVGDIDLTYNGI GPITSQCTNVRPIISGKQNPKI
Sbjct: 353 RHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNPKI 395

BLAST of CmaCh13G003120 vs. NCBI nr
Match: XP_022933298.1 (exopolygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 645.6 bits (1664), Expect = 2.3e-181
Identity = 316/343 (92.13%), Postives = 319/343 (93.00%), Query Frame = 0

Query: 1   NAWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGDNWITFE 60
           NAWK ACTSNRSSAIVIPGGI+KVKEGIFKGPCNSPIDFQLGGTLQAPKSLQG+NWITFE
Sbjct: 53  NAWKDACTSNRSSAIVIPGGIFKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGNNWITFE 112

Query: 61  HIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDSKNF 120
            IDRLTLSGEGVFDGQGKAAWKKND HK INCAQLPISLRFNFITNSIVKRITSLDSKNF
Sbjct: 113 RIDRLTLSGEGVFDGQGKAAWKKNDCHKRINCAQLPISLRFNFITNSIVKRITSLDSKNF 172

Query: 121 HINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNSFAITI----------------KQI 180
           HINVLGCNNLTFHNINITAPENSPNTDGIHIGRSN  AIT+                KQI
Sbjct: 173 HINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNRIAITMSKIATGDDCISLGDGSKQI 232

Query: 181 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 240
           NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN
Sbjct: 233 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 292

Query: 241 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKLICS 300
           MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIR SCATPVAIKLICS
Sbjct: 293 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRGSCATPVAIKLICS 352

Query: 301 RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPKI 328
           RHLPCDEVKVGDIDLTYNGI GPITSQCTNVRPIISGKQNPKI
Sbjct: 353 RHLPCDEVKVGDIDLTYNGIKGPITSQCTNVRPIISGKQNPKI 395

BLAST of CmaCh13G003120 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 373.2 bits (957), Expect = 2.1e-103
Identity = 180/343 (52.48%), Postives = 242/343 (70.55%), Query Frame = 0

Query: 2   AWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAP-KSLQG-DNWITF 61
           A+  AC S+  S +VIP G +K+ E   +GPC +PI+  L GT++A   ++QG + W+ F
Sbjct: 42  AFTSACQSSSPSKVVIPKGEFKLGEIEMRGPCKAPIEVTLQGTVKADGNAIQGKEKWVVF 101

Query: 62  EHIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDSKN 121
            +ID   L+G G FDG+G AAW+ N+ HK   C +LPIS+RF+FI NS ++ I+S+D+KN
Sbjct: 102 GNIDGFKLNGGGAFDGEGNAAWRVNNCHKTFECKKLPISIRFDFILNSEIRDISSIDAKN 161

Query: 122 FHINVLGCNNLTFHNINITAPENSPNTDGIHIGRS------NSFAIT----------IKQ 181
           FHINVLG  N+T +NI I APE+SPNTDGIH+GRS      NSF  T          +K 
Sbjct: 162 FHINVLGAKNMTMNNIKIVAPEDSPNTDGIHLGRSDGVKILNSFISTGDDCISVGDGMKN 221

Query: 182 INITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTAT 241
           +++  VTCGPGHGIS+GSLGRY +E+ V G+ V NCT+  T NG+RIKTWP++A S TA+
Sbjct: 222 LHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTLQETDNGLRIKTWPSAACSTTAS 281

Query: 242 NMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKLIC 301
           ++ F DII+ +VSNPILIDQEYCPWNQCN++  S IK+  +SFKNIR +     A+KL+C
Sbjct: 282 DIHFEDIILKDVSNPILIDQEYCPWNQCNKQKASTIKLVNISFKNIRGTSGNKDAVKLLC 341

Query: 302 SRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPK 327
           S+  PC  V++GDID+ YNG +GP T  C+NV P I G Q+PK
Sbjct: 342 SKGYPCQNVEIGDIDIKYNGADGPATFHCSNVSPKILGSQSPK 384

BLAST of CmaCh13G003120 vs. TAIR 10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 364.4 bits (934), Expect = 9.7e-101
Identity = 172/342 (50.29%), Postives = 238/342 (69.59%), Query Frame = 0

Query: 2   AWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQG-DNWITFE 61
           A+  AC     S ++IP G YK+ E +  GPC +PI   L GT++A  +  G + W+ F 
Sbjct: 43  AFNEACQFPTKSTVMIPKGEYKLGEIVMMGPCKAPIRIALLGTVKADGNANGKEKWVAFR 102

Query: 62  HIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDSKNF 121
           +I+   L+G GVFDG+G AAW+ N+ HK  NC +LPIS+RF+F+T++ ++ ITSLD+K+F
Sbjct: 103 NINGFKLNGGGVFDGEGNAAWRVNNCHKTFNCKKLPISIRFDFVTDAKIRGITSLDAKHF 162

Query: 122 HINVLGCNNLTFHNINITAPENSPNTDGIHIGRS------NSFAIT----------IKQI 181
           HINV+G  N+TF ++ I AP  SPNTDGIH+GRS      NSF  T          +K +
Sbjct: 163 HINVIGAKNVTFEDVKIIAPAESPNTDGIHVGRSDGIKIINSFISTGDDCVSVGDGMKNL 222

Query: 182 NITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTATN 241
            +  VTCGPGHGISIGSLGRY++EE V G+ + NCT+  T NG+RIKTWP++A + TA++
Sbjct: 223 LVERVTCGPGHGISIGSLGRYSHEENVSGIKIINCTLQETDNGLRIKTWPSAACTTTASD 282

Query: 242 MQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKLICS 301
           + F +I++ NVSNPILIDQEYCPWNQCN++ PS IK++ +SFK IR +     A+KL+CS
Sbjct: 283 IHFENILLKNVSNPILIDQEYCPWNQCNKQKPSTIKLANISFKKIRGTSGNKDAVKLLCS 342

Query: 302 RHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPK 327
           +  PC  V+VGD+++ Y G +GP T QC+NV P + G Q PK
Sbjct: 343 KGYPCQNVEVGDVNIQYTGADGPATFQCSNVSPKLVGTQIPK 384

BLAST of CmaCh13G003120 vs. TAIR 10
Match: AT3G07830.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 359.8 bits (922), Expect = 2.4e-99
Identity = 170/343 (49.56%), Postives = 234/343 (68.22%), Query Frame = 0

Query: 2   AWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAP-KSLQG-DNWITF 61
           A+  AC S     +VIP G +K+ E +  GPC SP++  L GT+ A   S+ G + W+ F
Sbjct: 43  AFTTACQSPTPRKVVIPKGQFKLGEIMMSGPCKSPVEITLLGTVLADGNSIHGKEKWVVF 102

Query: 62  EHIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDSKN 121
           + +D   L+G G FDG+G AAW+ N+ HK   C +LPIS+RF+F+TN+ ++ I+S+D+KN
Sbjct: 103 QRMDGFRLNGGGTFDGEGNAAWRVNNCHKTFECKKLPISIRFDFVTNAEIRDISSIDAKN 162

Query: 122 FHINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNSFAITIKQIN-------------- 181
           FHINV+G  N+TF N+ I AP  SPNTDGIH+GRS   +I   +I+              
Sbjct: 163 FHINVIGAKNMTFDNVKIMAPAESPNTDGIHLGRSVGVSIINSRISTGDDCVSVGDGMVN 222

Query: 182 --ITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTAT 241
             + NV CGPGHGIS+GSLGRY +E+ V G+ V NCT+  T NG+RIKTWP++A S TA+
Sbjct: 223 LLVKNVVCGPGHGISVGSLGRYGHEQDVSGIRVINCTLQETDNGLRIKTWPSAACSTTAS 282

Query: 242 NMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKLIC 301
           N+ F +II+ NVSNPILIDQEYCPWNQCN++  S IK++ +SF+ IR +     A+KL+C
Sbjct: 283 NIHFENIILRNVSNPILIDQEYCPWNQCNKQKSSSIKLANISFRRIRGTSGNKDAVKLLC 342

Query: 302 SRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPK 327
           S+  PC+ V+VGDI++ Y G +GP T  C+NVRP + G Q PK
Sbjct: 343 SKGYPCENVQVGDINIQYTGADGPATFMCSNVRPKLVGTQFPK 385

BLAST of CmaCh13G003120 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 358.2 bits (918), Expect = 6.9e-99
Identity = 169/343 (49.27%), Postives = 235/343 (68.51%), Query Frame = 0

Query: 2   AWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAP-KSLQG-DNWITF 61
           A+  AC +  +S +VI  G +K+ E    GPC +P++  L GTL+A  K++QG + W+ F
Sbjct: 43  AFTSACQAPTASKVVITKGEFKLGEIEMTGPCKAPVEINLQGTLKADGKAIQGKERWVVF 102

Query: 62  EHIDRLTLSGEGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLDSKN 121
             I+   L+G G+FDG+G AAW+ N+ HK   C +LPIS+RF+F+ N+ ++ I+S+D+KN
Sbjct: 103 LRINGFKLNGGGIFDGEGNAAWRVNNCHKTFECKKLPISIRFDFVENAEIRDISSIDAKN 162

Query: 122 FHINVLGCNNLTFHNINITAPENSPNTDGIHIGRSNSFAIT----------------IKQ 181
           FHINVLG  N+TF N+ + AP  SPNTDGIH+GRS    I                 +K 
Sbjct: 163 FHINVLGAKNMTFDNVKVIAPAESPNTDGIHLGRSEGVKILNSKIATGDDCISVGDGMKN 222

Query: 182 INITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASGTAT 241
           +++ NV CGPGHGIS+GSLGRY +E+ V G+ V NCT+  T NG+RIKTWP++A + TA+
Sbjct: 223 LHVENVMCGPGHGISVGSLGRYVHEQDVTGITVVNCTLQGTDNGLRIKTWPSAACATTAS 282

Query: 242 NMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIKLIC 301
            + F +II+ NVSNPILIDQEYCPWNQCN++ PS IK+  +SFKNIR +     A+KL+C
Sbjct: 283 GIHFENIILNNVSNPILIDQEYCPWNQCNKQKPSTIKLVDISFKNIRGTSGNKDAVKLLC 342

Query: 302 SRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNPK 327
           S+  PC  V++G+I+L Y G +GP T  C+NV P + G QNPK
Sbjct: 343 SKAHPCANVEIGNINLEYKGADGPPTFMCSNVSPKLVGTQNPK 385

BLAST of CmaCh13G003120 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 335.1 bits (858), Expect = 6.3e-92
Identity = 170/345 (49.28%), Postives = 220/345 (63.77%), Query Frame = 0

Query: 2   AWKVACTSNRSSAIVIPGGIYKVKEGIFKGPCNSPIDFQLGGTLQAPKSLQGD----NWI 61
           AWK AC +   S I +P G Y V+   FKGPC  P+  +L G  +AP +++       WI
Sbjct: 93  AWKEACAA--GSTITVPKGEYLVESLEFKGPCKGPVTLELNGNFKAPATVKTTKPHAGWI 152

Query: 62  TFEHIDRLTLSG-EGVFDGQGKAAWKKNDFHKHINCAQLPISLRFNFITNSIVKRITSLD 121
            FE++   TL+G + +FDGQG  AWK ND  K   C  LPI++RF  +TNS +  ITS +
Sbjct: 153 DFENLADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPINIRFTGLTNSKINSITSTN 212

Query: 122 SKNFHINVLGCNNLTFHNINITAPENSPNTDGIHIGRSN----------------SFAIT 181
           SK FH+N+L C N+T  +I I AP  S NTDGIHIGRSN                S    
Sbjct: 213 SKLFHMNILNCKNITLTDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDG 272

Query: 182 IKQINITNVTCGPGHGISIGSLGRYTNEEPVEGVIVNNCTIINTTNGVRIKTWPASAASG 241
            + + + NV CGPGHGISIGSLGRY NE+PV+GV V  C I NT NGVRIKTWP S   G
Sbjct: 273 TENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGS-PPG 332

Query: 242 TATNMQFLDIIMVNVSNPILIDQEYCPWNQCNRKIPSKIKISKVSFKNIRESCATPVAIK 301
            A+N+ F DI M NVS P+LIDQEYCP+  C   +PSK+K+S V+ KNI+ + AT VA+K
Sbjct: 333 IASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSKVKLSDVTIKNIKGTSATKVAVK 392

Query: 302 LICSRHLPCDEVKVGDIDLTYNGINGPITSQCTNVRPIISGKQNP 326
           L+CS+ +PC  + + DI+L +NG  GP  S C+N++PI+SGK  P
Sbjct: 393 LMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGKLVP 434

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P245484.7e-10053.18Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1[more]
Q059673.1e-9650.00Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1[more]
P353374.1e-9649.56Polygalacturonase OS=Brassica napus OX=3708 PE=2 SV=1[more]
Q397867.7e-9548.55Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1[more]
Q6H9K01.7e-9447.69Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1HYN03.7e-18593.29exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111469285 PE=3 SV=1[more]
A0A6J1I5223.7e-18593.29exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111469287 PE=3 SV=1[more]
A0A6J1I2E91.6e-18393.00exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111469289 PE=3 SV=1[more]
A0A6J1EHA11.1e-18192.13exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111434295 PE=3 SV=... [more]
A0A6J1F4C61.1e-18192.13exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111440472 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_022970272.17.7e-18593.29exopolygalacturonase-like [Cucurbita maxima] >XP_022970273.1 exopolygalacturonas... [more]
XP_022970274.17.7e-18593.29exopolygalacturonase-like [Cucurbita maxima][more]
XP_022970275.13.2e-18393.00exopolygalacturonase-like [Cucurbita maxima][more]
XP_022927477.12.3e-18192.13exopolygalacturonase-like [Cucurbita moschata][more]
XP_022933298.12.3e-18192.13exopolygalacturonase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G07820.12.1e-10352.48Pectin lyase-like superfamily protein [more]
AT5G48140.19.7e-10150.29Pectin lyase-like superfamily protein [more]
AT3G07830.12.4e-9949.56Pectin lyase-like superfamily protein [more]
AT3G07840.16.9e-9949.27Pectin lyase-like superfamily protein [more]
AT3G14040.16.3e-9249.28Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 127..153
e-value: 36.0
score: 13.3
coord: 156..181
e-value: 1700.0
score: 5.6
coord: 191..212
e-value: 2.3
score: 17.2
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 7..316
e-value: 5.6E-81
score: 272.1
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 168..181
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 1..325
e-value: 1.8E-79
score: 269.6
NoneNo IPR availablePANTHERPTHR31375:SF248POLYGALACTURONASEcoord: 1..319
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 1..319
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 1..325

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh13G003120.1CmaCh13G003120.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity