CmaCh13G002450 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh13G002450
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionCarbonic anhydrase
LocationCma_Chr13: 2033966 .. 2037421 (+)
RNA-Seq ExpressionCmaCh13G002450
SyntenyCmaCh13G002450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGTAAATTCTCTAGAGATCAAACACAGTTCGTATTAGGACATGGAGAAGCTCTTGTGTTCTTTCTTCTTTTGCTTTCTTTTGCTTTCATTTAAGGCACAATCCCAAGAAGTTGGTGAGAGATTCATACTACATTGTTTTTTACGTACTTTCATTTTATGTATATATATATATATATTGATTTGACACTTTTATGTTTTAAATGTATTTAATTATACTTCGATGATGATAAATGTAAGAGCTCAAACTCACTGCTTGTAGATATTGTCTTTTTTGAGCTTTCCCTTTATAAAGAATGCTCCGTTCCCCTCTCCAACCGATGTGGGATCTCACAATTCACCCCCTTCGGGGACCCAACGTCCTCTTGGTACTCGTTGCTCTCTCGAATTAATGTGGGATCTCACAATCCACCCCCTTCGGAGGCCCTACATCCTTGTTGGCACACCTCCCGGTGTCCACCCTCTTCGGGGCTCAGTGTCCTGATGGCACACCGCCTAGTGTCTAGCTCTAATACTATTTGTAATAGCCTAATCCCACCACTAGTAGATATTGTCCTCTTTGGACTTTCCCTTTTCAGGCTTCCCCTTAAGGTCTTTATAACGGTATGCTAGGAGAGGTTTCTGCATTTGTTCTCCTCTCCAATTGATGTGAGATCTTAAAGTAAAATTATTTGAAATGATACGGATAAAGAATTGGTAGAGGAGATAGATCTATTTGTTGGAGTCCTCAAAGGTGTAGTCATAGTGAAAAATCATGGTAGAGCTCTATTGATCTAACAATGTACTCTAGAAAATAGAAGTTGTCTATAAGAATAGTGCTAGACATATAGATGATTGTTCAAAAGGGAGGCTCAATGACATTATTTGTTCAAGAAGATAATGGTTAGAATCCTAAGTTGACTAATTAAGAGAAAGATTCTAGATATACGAGTGAGGACCCGTGTCTTCAGGGTTATTGAAGAAACCAAAAGAAAAGTTATGAGAGTATGGGCAAACTAGATTCGTTGTCCTATAGCTATTTTTCCTTCTAATTTATGATAAATACCAGTAGAAATTTCTAACTTTTTATTCCAAAGTAAGCGAATACATCCACTAATTTTGTAAACTAAGCTATATATTAGCATTTTGTTTCCGTTGATGATGTCTTTTTCCCTTCTTAATTGGTTTAATGATTATATGTTCATTGCTTATGTAGAGGATGAAAGAGCATTTGATTATGAATCCGATGGAATAAAAGGACCGACTCATTGGGGAGAGCTTCGACCAGAGTGGCATGCATGCAAGGCTGGTTTAATGCAATCTCCCATCGATATGTTGCATGAAAGAGTTGAAGTTATTTCGCATCTTGGAGACCTCAAAATCAACTACAAACCCTCTAATGCCACTCTCAAGAACAGAGGCCATGATATGATGGTAAACAACGAAAAACTCTAAAAACAACCCTTTAGAAGATATTGTACTCTTGAATTTACTATCTTCCGGCTTATTTACATATTATATGGTTAAATTTATGTTAGTATAATGACGCGTAGACCGTAATGGTTAAATTTATGTTATCCCACATTGGTTAAGAAGGAGAACGAAACATTATTCATAAGAGTGTAGAAACCTTTCTCTAGTAGACGCATTTTAAAAACTATGAGATCCCACATTGATTAGGGAGGAGAATGGAACACGCTTAGGGTGTGGAAACCCGAGGATGCTGAGCCTTGAAAGGGGAGTGGATTGTAAGATCCCACATCGGTTGGGGAGGAGAAAAAGTTGAAACTTTTCCCTATGTGTATAAAAATCTTTGAGGGGAAGTCCAAGAAAGAAAGTTCAAAGAGGACAATATCTACTAGCGGTGGACTTACTAGTTAACCTATTAGTGAAATATTTGAATTGTTGAGCAGATGAAATGGATAGAAGGAGGTGGATTCATGGAAGTAAATGGAATGCAATATAAGCTCAAACAATGTCATTGGCACTCACCATCTGAGCACACCATTGACGGTAAGAAGTTTGATCTTGAAGCTCACTTGGTCCATGAAAGCTCAAATGGAATGGTTTCTGTAATTGGAATCTTGTATCAGATTGGAGAGCCTGATTATTTCTTATCAACTGTGAGTATATTTTCATAATCTTCAAGAATTATCATTATAAATCATATACTTATTACAATTTTATTTCCTATTAGATAAAAGATCATTTGGAAGTCGTCTCCGATAGCCATGAAGAGGAGAGAATAATCGGAGTAATCGATCCAACTCTGATAAAGATAGGCAGCAAACGATATTATAGATATATGGGCTCGCTTACCGTTCCACCTTGTACTGAAAGTGTAATGTGGACTATGATCCATGAGGTATTCTTCGAATTTAATACTAGTTTAATATTATTTTATTTAAAAGATATTAGTCAATTCGAGCATAACTCAATTGCTCATGTGAATAAAATATATATTTTCAATTACGAGGTAAGACGTTTGGATCGGATACCCATATTTTTGAATTATACAATCCAGTTACATTCGGTTAGTCATAGAAAAAATATATTTGCAAGTTAAACTATACTCATATTAATAATTTGATTAACCGTTTAAAAATACACTTGATAGGATAAAAAAAAAATTAAAATAAAGTTGTAACTTAGAGCTTATTTTTTATTATTAATTTTTTTTCAATAGTTTTTTTTAGCTTTTAATATTTTATTTTTTTATTTTTGGTTTATGAAATTTAAACATATTAACAATTTCTTTGTTGAGTGTTAGCAATATTCCAAAATGGTTTCAAAATTTAAGTTTGGAAAAGAAAAAAAGTAATTTTGAAAAACTATATTTTTATTTATGGAAATTTAAATATTTACTTGATGAAAGTATTATAAATAAAAATTAAGAAAATAGTCACAATTTTTTAAAAACTAAAAACTAAAATTAAATTATTATCATACATTTGCAATTACTTAATTTATTTTCATTTATTTTCATTTCAGGTGAGGACCGTAACTGAAGAACAAGTGAAATTACTTCGAGTAGCTGTTCATGATGTAAGTTATAAATTAATTGAGATTAAAAAAATTAAATAAAAAAAATAAAAGTCCTCTACTAACCCATTATAAATTATATTTGAAAGTTTATGAATATTTTTTAAATAAGTTGCGAATAATAAAGATGTTTTTGAACTAGCCTTAAAAAAAAAAAAAAAAAAAAAATCAAATTTATAACTAAACGTAGCTATCTAAATTGGTATTAATTTTATACATTCATATTTGTTGATATCAAAAGTGTATATATAATTACAGGACTCGGATACTAATGCAAGACCATTGCAACCAATCTATGATCGAAAAACACAACTCCAAAGGCCTAAGGAAAGTGAAGTTTGACAAGAACGAATTTTAGTTCGCCATTTAGGCTATCTTGTCAATCCCTACTCTTCATATTTGTATTTTGTTGTTTATATCAATATTTCGTACCCTTG

mRNA sequence

TTGTAAATTCTCTAGAGATCAAACACAGTTCGTATTAGGACATGGAGAAGCTCTTGTGTTCTTTCTTCTTTTGCTTTCTTTTGCTTTCATTTAAGGCACAATCCCAAGAAGTTGAGGATGAAAGAGCATTTGATTATGAATCCGATGGAATAAAAGGACCGACTCATTGGGGAGAGCTTCGACCAGAGTGGCATGCATGCAAGGCTGGTTTAATGCAATCTCCCATCGATATGTTGCATGAAAGAGTTGAAGTTATTTCGCATCTTGGAGACCTCAAAATCAACTACAAACCCTCTAATGCCACTCTCAAGAACAGAGGCCATGATATGATGATGAAATGGATAGAAGGAGGTGGATTCATGGAAGTAAATGGAATGCAATATAAGCTCAAACAATGTCATTGGCACTCACCATCTGAGCACACCATTGACGGTAAGAAGTTTGATCTTGAAGCTCACTTGGTCCATGAAAGCTCAAATGGAATGGTTTCTGTAATTGGAATCTTGTATCAGATTGGAGAGCCTGATTATTTCTTATCAACTATAAAAGATCATTTGGAAGTCGTCTCCGATAGCCATGAAGAGGAGAGAATAATCGGAGTAATCGATCCAACTCTGATAAAGATAGGCAGCAAACGATATTATAGATATATGGGCTCGCTTACCGTTCCACCTTGTACTGAAAGTGTAATGTGGACTATGATCCATGAGGTGAGGACCGTAACTGAAGAACAAGTGAAATTACTTCGAGTAGCTGTTCATGATGACTCGGATACTAATGCAAGACCATTGCAACCAATCTATGATCGAAAAACACAACTCCAAAGGCCTAAGGAAAGTGAAGTTTGACAAGAACGAATTTTAGTTCGCCATTTAGGCTATCTTGTCAATCCCTACTCTTCATATTTGTATTTTGTTGTTTATATCAATATTTCGTACCCTTG

Coding sequence (CDS)

ATGGAGAAGCTCTTGTGTTCTTTCTTCTTTTGCTTTCTTTTGCTTTCATTTAAGGCACAATCCCAAGAAGTTGAGGATGAAAGAGCATTTGATTATGAATCCGATGGAATAAAAGGACCGACTCATTGGGGAGAGCTTCGACCAGAGTGGCATGCATGCAAGGCTGGTTTAATGCAATCTCCCATCGATATGTTGCATGAAAGAGTTGAAGTTATTTCGCATCTTGGAGACCTCAAAATCAACTACAAACCCTCTAATGCCACTCTCAAGAACAGAGGCCATGATATGATGATGAAATGGATAGAAGGAGGTGGATTCATGGAAGTAAATGGAATGCAATATAAGCTCAAACAATGTCATTGGCACTCACCATCTGAGCACACCATTGACGGTAAGAAGTTTGATCTTGAAGCTCACTTGGTCCATGAAAGCTCAAATGGAATGGTTTCTGTAATTGGAATCTTGTATCAGATTGGAGAGCCTGATTATTTCTTATCAACTATAAAAGATCATTTGGAAGTCGTCTCCGATAGCCATGAAGAGGAGAGAATAATCGGAGTAATCGATCCAACTCTGATAAAGATAGGCAGCAAACGATATTATAGATATATGGGCTCGCTTACCGTTCCACCTTGTACTGAAAGTGTAATGTGGACTATGATCCATGAGGTGAGGACCGTAACTGAAGAACAAGTGAAATTACTTCGAGTAGCTGTTCATGATGACTCGGATACTAATGCAAGACCATTGCAACCAATCTATGATCGAAAAACACAACTCCAAAGGCCTAAGGAAAGTGAAGTTTGA

Protein sequence

MEKLLCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQSPIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHEEERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVHDDSDTNARPLQPIYDRKTQLQRPKESEV
Homology
BLAST of CmaCh13G002450 vs. ExPASy Swiss-Prot
Match: Q8L817 (Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 1.5e-83
Identity = 141/259 (54.44%), Postives = 195/259 (75.29%), Query Frame = 0

Query: 5   LCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQSPIDM 64
           L S F    + S  +   EVEDER F+Y+ +  KGP  WGEL+PEW  C  G MQSPID+
Sbjct: 15  LFSIFTIVSISSAASSHGEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQSPIDL 74

Query: 65  LHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCHWHSP 124
           ++ERV ++SHLG L  +Y PSNATLKNRGHD+M+K+ +G G +++NG +Y+L+Q HWHSP
Sbjct: 75  MNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQLHWHSP 134

Query: 125 SEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHEEERI 184
           SEHTI+G++F LE H+VHE  N  ++V+ +LY+IG  D F+ +++  LE +++  E E+ 
Sbjct: 135 SEHTINGRRFALELHMVHEGRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAEMEEAEKN 194

Query: 185 IGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVHDDSD 244
           +G+IDPT IKIGS++YYRY GSLT PPCT++V W+++ +VRTVT +QVKLLRVAVHDD++
Sbjct: 195 VGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRVAVHDDAN 254

Query: 245 TNARPLQPIYDRKTQLQRP 264
           +NARP+QP   R   L RP
Sbjct: 255 SNARPVQPTNKRIVHLYRP 273

BLAST of CmaCh13G002450 vs. ExPASy Swiss-Prot
Match: Q84UV8 (Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS=Nicotiana langsdorffii x Nicotiana sanderae OX=164110 GN=NEC3 PE=1 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.4e-73
Identity = 137/251 (54.58%), Postives = 186/251 (74.10%), Query Frame = 0

Query: 9   FFCFLLLS--FKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQSPIDMLH 68
           F  FL LS  F A+S EV+DE  F Y+     GP +WG +RP+W  C +G +QSPID+  
Sbjct: 11  FISFLFLSSVFLARSGEVDDESEFSYDEKSENGPANWGNIRPDWKEC-SGKLQSPIDIFD 70

Query: 69  ERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCHWHSPSE 128
            R EV+S+L  L+ +YKPSNATL NRGHD+M++ ++ GG++++N  QY+LKQ HWH+PSE
Sbjct: 71  LRAEVVSNLRILQKDYKPSNATLLNRGHDIMLR-LDDGGYLKINETQYQLKQLHWHTPSE 130

Query: 129 HTIDGKKFDLEAHLVHESSNGMVSVIGILYQIG-EPDYFLSTIKDHLEVVSDSHEEERII 188
           HTI+G++F+LEAHLVHES+NG   VIGI+Y+IG  PD FLS I++ L+V ++    ER I
Sbjct: 131 HTINGERFNLEAHLVHESNNGKFVVIGIVYEIGLWPDPFLSMIENDLKVPANKKGIERGI 190

Query: 189 GVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVHDDSDT 248
           G+IDP  IK+  K+Y+RY+GSLT PPCTE V+W +  +V+TVT  Q+KLL+ AVHD  +T
Sbjct: 191 GIIDPNQIKLDGKKYFRYIGSLTTPPCTEGVVWIIDRKVKTVTRRQIKLLQEAVHDGFET 250

Query: 249 NARPLQPIYDR 257
           NARP QP  +R
Sbjct: 251 NARPTQPENER 259

BLAST of CmaCh13G002450 vs. ExPASy Swiss-Prot
Match: F4HUC4 (Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 9.8e-72
Identity = 130/262 (49.62%), Postives = 184/262 (70.23%), Query Frame = 0

Query: 8   FFFCFLLLSFKAQSQ---EVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQSPIDM 67
           FF  F+ ++  + S    EVEDE  F+YE  G KGP +WG L+PEW  C  G MQSPID+
Sbjct: 10  FFLIFISITIVSSSPDHGEVEDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQSPIDL 69

Query: 68  LHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGF---MEVNGMQYKLKQCHW 127
             +RV +  +LG L+  Y PSNAT+KNRGHD+MMK+ EGG     + +NG +YKL+Q HW
Sbjct: 70  TDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIMMKF-EGGNAGLGITINGTEYKLQQIHW 129

Query: 128 HSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHEE 187
           HSPSEHT++GK+F LE H+VH+S +G  +V+   Y++G+PDYFL T++ +L+ ++D+HE 
Sbjct: 130 HSPSEHTLNGKRFVLEEHMVHQSKDGRNAVVAFFYKLGKPDYFLLTLERYLKRITDTHES 189

Query: 188 ERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVHD 247
           +  + ++ P      SK YYR++GSLT PPC+E+V+WT+  E+RTVT +Q+ +LRV VHD
Sbjct: 190 QEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMRTVTLKQLIMLRVTVHD 249

Query: 248 DSDTNARPLQPIYDRKTQLQRP 264
            S++NARPLQ   +R   L  P
Sbjct: 250 QSNSNARPLQRKNERPVALYIP 270

BLAST of CmaCh13G002450 vs. ExPASy Swiss-Prot
Match: Q9FM99 (Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3)

HSP 1 Score: 268.5 bits (685), Expect = 8.3e-71
Identity = 129/248 (52.02%), Postives = 170/248 (68.55%), Query Frame = 0

Query: 19  AQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQSPIDMLHERVEVISHLGDL 78
           A   EVEDE  F YE+ G KGP  WG L  EW  C  G MQSPID+  + V V +  G L
Sbjct: 129 APGGEVEDETEFSYETKGNKGPAKWGTLDAEWKMCGIGKMQSPIDLRDKNVVVSNKFGLL 188

Query: 79  KINYKPSNATLKNRGHDMMMKWIEG--GGFMEVNGMQYKLKQCHWHSPSEHTIDGKKFDL 138
           +  Y PSN T+KNRGHD+M+K+  G  G  + + G +Y+L+Q HWHSPSEHTI+GK+F L
Sbjct: 189 RSQYLPSNTTIKNRGHDIMLKFKGGNKGIGVTIRGTRYQLQQLHWHSPSEHTINGKRFAL 248

Query: 139 EAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHEEERIIGVIDPTLIKIG 198
           E HLVHES +   +V+  LY +G  D FL +++  L+ ++D+H  E  +G+IDP  +   
Sbjct: 249 EEHLVHESKDKRYAVVAFLYNLGASDPFLFSLEKQLKKITDTHASEEHVGIIDPKKLSFE 308

Query: 199 SKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVHDDSDTNARPLQPIYDR 258
           SK YYRY GSLT PPC+E+V+W++  E+RTV+ +QVKLLRVAVHD SD+NARPLQ +  R
Sbjct: 309 SKHYYRYSGSLTAPPCSENVIWSVSKEIRTVSSKQVKLLRVAVHDASDSNARPLQAVNKR 368

Query: 259 KTQLQRPK 265
           K  L +PK
Sbjct: 369 KVYLYKPK 376

BLAST of CmaCh13G002450 vs. ExPASy Swiss-Prot
Match: F4IHR4 (Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana OX=3702 GN=ACA2 PE=2 SV=2)

HSP 1 Score: 256.5 bits (654), Expect = 3.3e-67
Identity = 118/224 (52.68%), Postives = 163/224 (72.77%), Query Frame = 0

Query: 4   LLCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQSPID 63
           +L SF      LS     +EVEDE  F YE +   GP  WG+LRPEW  C  G MQSPID
Sbjct: 13  VLTSFVTTVSCLSAATDYREVEDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPID 72

Query: 64  MLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKW-IEGGGFMEVNGMQYKLKQCHWH 123
           ++++RV +++HL  L  +YKP NATLKNRGHDMM+K+  EG G + VNG +YKL Q HWH
Sbjct: 73  LMNKRVRLVTHLKKLTRHYKPCNATLKNRGHDMMLKFGEEGSGSITVNGTEYKLLQLHWH 132

Query: 124 SPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHEEE 183
           SPSEHT++G++F LE H+VHE+ NG ++V+ +LY+IG PD FL  +++ L  ++D +E E
Sbjct: 133 SPSEHTMNGRRFALELHMVHENINGSLAVVTVLYKIGRPDSFLGLLENKLSAITDQNEAE 192

Query: 184 RIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRT 227
           + + VIDP  IKIGS+++YRY+GSLT PPCT++V+WT++ +V T
Sbjct: 193 KYVDVIDPRDIKIGSRKFYRYIGSLTTPPCTQNVIWTVVKKVNT 236

BLAST of CmaCh13G002450 vs. ExPASy TrEMBL
Match: A0A6J1I0A8 (Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111469326 PE=3 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 7.4e-155
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 1   MEKLLCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60
           MEKLLCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS
Sbjct: 1   MEKLLCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60

Query: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCH 120
           PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCH
Sbjct: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCH 120

Query: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHE 180
           WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHE
Sbjct: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHE 180

Query: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240
           EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH
Sbjct: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240

Query: 241 DDSDTNARPLQPIYDRKTQLQRPKESEV 269
           DDSDTNARPLQPIYDRKTQLQRPKESEV
Sbjct: 241 DDSDTNARPLQPIYDRKTQLQRPKESEV 268

BLAST of CmaCh13G002450 vs. ExPASy TrEMBL
Match: A0A6J1HPL0 (Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111464882 PE=3 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 6.9e-153
Identity = 264/268 (98.51%), Postives = 266/268 (99.25%), Query Frame = 0

Query: 1   MEKLLCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60
           MEKLLCSFFFC LLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS
Sbjct: 1   MEKLLCSFFFCILLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60

Query: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCH 120
           PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKW+EGGGFMEVNGMQYKLKQCH
Sbjct: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWVEGGGFMEVNGMQYKLKQCH 120

Query: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHE 180
           WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVS SHE
Sbjct: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSGSHE 180

Query: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240
           EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH
Sbjct: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240

Query: 241 DDSDTNARPLQPIYDRKTQLQRPKESEV 269
           DDSDTNARPLQPIYDRKTQLQ+PKESEV
Sbjct: 241 DDSDTNARPLQPIYDRKTQLQKPKESEV 268

BLAST of CmaCh13G002450 vs. ExPASy TrEMBL
Match: A0A6J1DHJ2 (Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111020525 PE=3 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 1.2e-133
Identity = 224/267 (83.90%), Postives = 250/267 (93.63%), Query Frame = 0

Query: 1   MEKLLCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60
           M K LCSFFFC LLLSFKA SQEVE+ER FDYE DG +GP+HWG+LRPEW  CKAG MQS
Sbjct: 1   MGKFLCSFFFCILLLSFKAASQEVENEREFDYEDDGERGPSHWGDLRPEWQKCKAGFMQS 60

Query: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCH 120
           PID+LH+RVEVISHLGDL+ NY PSNAT+KNRGHD+MMKW++GGG+ME+NG QYKLKQCH
Sbjct: 61  PIDLLHQRVEVISHLGDLETNYIPSNATIKNRGHDIMMKWVDGGGYMEMNGXQYKLKQCH 120

Query: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHE 180
           WHSPSEHTIDGK FDLEAHLVHESS G++SVIGILYQIGEPDYFLST+KDHLEV+SD+HE
Sbjct: 121 WHSPSEHTIDGKNFDLEAHLVHESSTGIISVIGILYQIGEPDYFLSTMKDHLEVISDTHE 180

Query: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240
           EERIIG+IDPTL+KIGSK+YYRY+GSLTVPPCTE+V+WTMI EVRTVTEEQVK LRVAVH
Sbjct: 181 EERIIGIIDPTLLKIGSKQYYRYIGSLTVPPCTENVIWTMIREVRTVTEEQVKQLRVAVH 240

Query: 241 DDSDTNARPLQPIYDRKTQLQRPKESE 268
           DDSDTNARPLQPIY+RKTQLQRPKE+E
Sbjct: 241 DDSDTNARPLQPIYNRKTQLQRPKENE 267

BLAST of CmaCh13G002450 vs. ExPASy TrEMBL
Match: A0A6J1DG52 (Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111020526 PE=3 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 2.3e-132
Identity = 223/267 (83.52%), Postives = 247/267 (92.51%), Query Frame = 0

Query: 1   MEKLLCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60
           M KLLCSFFFC LLLSFKA SQEV+DER FDYE DG KGP+HWGEL+ EWH CKAG MQS
Sbjct: 1   MGKLLCSFFFCILLLSFKAASQEVDDEREFDYERDGEKGPSHWGELKAEWHKCKAGSMQS 60

Query: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCH 120
           PID+ H+RVEVISHLGDLK NY PSNAT+KNRGHD+MMKW++GGG+ME+NG QYKLKQCH
Sbjct: 61  PIDLYHQRVEVISHLGDLKTNYIPSNATIKNRGHDIMMKWVDGGGYMEMNGTQYKLKQCH 120

Query: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHE 180
           WHSPSEHTIDGK FDLEAHLVHESS G++S IGILYQIGEPDYFLST+KDHLEV+SD+HE
Sbjct: 121 WHSPSEHTIDGKNFDLEAHLVHESSTGIISAIGILYQIGEPDYFLSTMKDHLEVISDTHE 180

Query: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240
           EER IG+IDPTL+KIGSK+YYRY+GSLTVPPCTE+V+WTMI+EVRTVTEEQVK LRVAVH
Sbjct: 181 EERAIGIIDPTLLKIGSKQYYRYIGSLTVPPCTENVIWTMIYEVRTVTEEQVKSLRVAVH 240

Query: 241 DDSDTNARPLQPIYDRKTQLQRPKESE 268
           DDSDTNARPLQPIY+R  QLQRPKESE
Sbjct: 241 DDSDTNARPLQPIYNRTMQLQRPKESE 267

BLAST of CmaCh13G002450 vs. ExPASy TrEMBL
Match: A0A1S4E3N8 (Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103502435 PE=3 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 7.0e-121
Identity = 203/243 (83.54%), Postives = 226/243 (93.00%), Query Frame = 0

Query: 25  EDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQSPIDMLHERVEVISHLGDLKINYKP 84
           EDER FDYE DG KGP HWGELR EWH CK GLMQSPID+LHERVEVISHLGDL+INYK 
Sbjct: 6   EDERGFDYERDGEKGPVHWGELRAEWHKCKGGLMQSPIDLLHERVEVISHLGDLQINYKS 65

Query: 85  SNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCHWHSPSEHTIDGKKFDLEAHLVHES 144
           SNATLKNRGHDMMMKW++GGG+ME+NG QYKLKQCHWHSPSEHTIDGK FDLEAHLVHES
Sbjct: 66  SNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKNFDLEAHLVHES 125

Query: 145 SNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHEEERIIGVIDPTLIKIGSKRYYRYM 204
           SNGMVSVIGILYQIGEPDYFLSTIK+ LEV S++HEEER++G+IDP ++K+GSK+YYRY+
Sbjct: 126 SNGMVSVIGILYQIGEPDYFLSTIKEDLEVASNTHEEERVVGIIDPNILKMGSKQYYRYI 185

Query: 205 GSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVHDDSDTNARPLQPIYDRKTQLQRPK 264
           GSLTVPPCTE VMWT+IH+VRTVTEEQVKLLRVAVHDDS++NARPLQPIY+R  QLQR K
Sbjct: 186 GSLTVPPCTEDVMWTIIHQVRTVTEEQVKLLRVAVHDDSNSNARPLQPIYNRTMQLQRSK 245

Query: 265 ESE 268
           +SE
Sbjct: 246 DSE 248

BLAST of CmaCh13G002450 vs. NCBI nr
Match: XP_022970331.1 (alpha carbonic anhydrase 7-like [Cucurbita maxima])

HSP 1 Score: 556.2 bits (1432), Expect = 1.5e-154
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 1   MEKLLCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60
           MEKLLCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS
Sbjct: 1   MEKLLCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60

Query: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCH 120
           PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCH
Sbjct: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCH 120

Query: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHE 180
           WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHE
Sbjct: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHE 180

Query: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240
           EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH
Sbjct: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240

Query: 241 DDSDTNARPLQPIYDRKTQLQRPKESEV 269
           DDSDTNARPLQPIYDRKTQLQRPKESEV
Sbjct: 241 DDSDTNARPLQPIYDRKTQLQRPKESEV 268

BLAST of CmaCh13G002450 vs. NCBI nr
Match: KAG6583572.1 (Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 552.4 bits (1422), Expect = 2.2e-153
Identity = 265/268 (98.88%), Postives = 267/268 (99.63%), Query Frame = 0

Query: 1   MEKLLCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60
           MEKLLCSFFFC LLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS
Sbjct: 1   MEKLLCSFFFCILLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60

Query: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCH 120
           PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKW+EGGGFMEVNGMQYKLKQCH
Sbjct: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWVEGGGFMEVNGMQYKLKQCH 120

Query: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHE 180
           WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHE
Sbjct: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHE 180

Query: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240
           EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH
Sbjct: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240

Query: 241 DDSDTNARPLQPIYDRKTQLQRPKESEV 269
           DDSDTNARPLQPIYDRKTQLQ+PKESEV
Sbjct: 241 DDSDTNARPLQPIYDRKTQLQKPKESEV 268

BLAST of CmaCh13G002450 vs. NCBI nr
Match: XP_023520292.1 (alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 550.1 bits (1416), Expect = 1.1e-152
Identity = 264/268 (98.51%), Postives = 266/268 (99.25%), Query Frame = 0

Query: 1   MEKLLCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60
           MEKLLCSFFFC LLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS
Sbjct: 1   MEKLLCSFFFCILLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60

Query: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCH 120
           PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKW+EGGGFMEVNGMQYKLKQCH
Sbjct: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWVEGGGFMEVNGMQYKLKQCH 120

Query: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHE 180
           WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLE+VSDSHE
Sbjct: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLELVSDSHE 180

Query: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240
           EERIIGVIDPT IKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH
Sbjct: 181 EERIIGVIDPTRIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240

Query: 241 DDSDTNARPLQPIYDRKTQLQRPKESEV 269
           DDSDTNARPLQPIYDRKTQLQRPKESEV
Sbjct: 241 DDSDTNARPLQPIYDRKTQLQRPKESEV 268

BLAST of CmaCh13G002450 vs. NCBI nr
Match: XP_022964924.1 (alpha carbonic anhydrase 7-like [Cucurbita moschata])

HSP 1 Score: 549.7 bits (1415), Expect = 1.4e-152
Identity = 264/268 (98.51%), Postives = 266/268 (99.25%), Query Frame = 0

Query: 1   MEKLLCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60
           MEKLLCSFFFC LLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS
Sbjct: 1   MEKLLCSFFFCILLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60

Query: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCH 120
           PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKW+EGGGFMEVNGMQYKLKQCH
Sbjct: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWVEGGGFMEVNGMQYKLKQCH 120

Query: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHE 180
           WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVS SHE
Sbjct: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSGSHE 180

Query: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240
           EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH
Sbjct: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240

Query: 241 DDSDTNARPLQPIYDRKTQLQRPKESEV 269
           DDSDTNARPLQPIYDRKTQLQ+PKESEV
Sbjct: 241 DDSDTNARPLQPIYDRKTQLQKPKESEV 268

BLAST of CmaCh13G002450 vs. NCBI nr
Match: XP_038895739.1 (alpha carbonic anhydrase 7-like [Benincasa hispida])

HSP 1 Score: 486.1 bits (1250), Expect = 1.9e-133
Identity = 228/267 (85.39%), Postives = 248/267 (92.88%), Query Frame = 0

Query: 1   MEKLLCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60
           M KLLCSFFF  LLLSFKA+SQEV+DER FDYE DG KGP HWGELR EWH CK GLMQS
Sbjct: 1   MGKLLCSFFFSILLLSFKARSQEVDDERGFDYEGDGEKGPIHWGELRAEWHTCKVGLMQS 60

Query: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCH 120
           PID+LHERVEVISHLGDLKINYK SNATLKNRGHDMMMKW++GGG+ME+NG QYKLKQCH
Sbjct: 61  PIDLLHERVEVISHLGDLKINYKSSNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCH 120

Query: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHE 180
           WHSPSEHTIDGKKFDLEAHLVHESSNGM+SVIGILYQIGEPDYFLSTIK  LE+VSD+HE
Sbjct: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKGDLEMVSDTHE 180

Query: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240
           EER+IG+IDP L+K+GSK+YYRY+GSLTVPPCTE+VMWTMIH+VRTVTEEQVKLLRVAVH
Sbjct: 181 EERVIGIIDPNLLKMGSKQYYRYIGSLTVPPCTENVMWTMIHQVRTVTEEQVKLLRVAVH 240

Query: 241 DDSDTNARPLQPIYDRKTQLQRPKESE 268
           DDSD+NARPLQPIY+R  QLQ  KESE
Sbjct: 241 DDSDSNARPLQPIYNRTMQLQTSKESE 267

BLAST of CmaCh13G002450 vs. TAIR 10
Match: AT1G08080.1 (alpha carbonic anhydrase 7 )

HSP 1 Score: 310.8 bits (795), Expect = 1.0e-84
Identity = 141/259 (54.44%), Postives = 195/259 (75.29%), Query Frame = 0

Query: 5   LCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQSPIDM 64
           L S F    + S  +   EVEDER F+Y+ +  KGP  WGEL+PEW  C  G MQSPID+
Sbjct: 15  LFSIFTIVSISSAASSHGEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQSPIDL 74

Query: 65  LHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCHWHSP 124
           ++ERV ++SHLG L  +Y PSNATLKNRGHD+M+K+ +G G +++NG +Y+L+Q HWHSP
Sbjct: 75  MNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQLHWHSP 134

Query: 125 SEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHEEERI 184
           SEHTI+G++F LE H+VHE  N  ++V+ +LY+IG  D F+ +++  LE +++  E E+ 
Sbjct: 135 SEHTINGRRFALELHMVHEGRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAEMEEAEKN 194

Query: 185 IGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVHDDSD 244
           +G+IDPT IKIGS++YYRY GSLT PPCT++V W+++ +VRTVT +QVKLLRVAVHDD++
Sbjct: 195 VGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRVAVHDDAN 254

Query: 245 TNARPLQPIYDRKTQLQRP 264
           +NARP+QP   R   L RP
Sbjct: 255 SNARPVQPTNKRIVHLYRP 273

BLAST of CmaCh13G002450 vs. TAIR 10
Match: AT1G08065.1 (alpha carbonic anhydrase 5 )

HSP 1 Score: 271.6 bits (693), Expect = 7.0e-73
Identity = 130/262 (49.62%), Postives = 184/262 (70.23%), Query Frame = 0

Query: 8   FFFCFLLLSFKAQSQ---EVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQSPIDM 67
           FF  F+ ++  + S    EVEDE  F+YE  G KGP +WG L+PEW  C  G MQSPID+
Sbjct: 10  FFLIFISITIVSSSPDHGEVEDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQSPIDL 69

Query: 68  LHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGF---MEVNGMQYKLKQCHW 127
             +RV +  +LG L+  Y PSNAT+KNRGHD+MMK+ EGG     + +NG +YKL+Q HW
Sbjct: 70  TDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIMMKF-EGGNAGLGITINGTEYKLQQIHW 129

Query: 128 HSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHEE 187
           HSPSEHT++GK+F LE H+VH+S +G  +V+   Y++G+PDYFL T++ +L+ ++D+HE 
Sbjct: 130 HSPSEHTLNGKRFVLEEHMVHQSKDGRNAVVAFFYKLGKPDYFLLTLERYLKRITDTHES 189

Query: 188 ERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVHD 247
           +  + ++ P      SK YYR++GSLT PPC+E+V+WT+  E+RTVT +Q+ +LRV VHD
Sbjct: 190 QEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMRTVTLKQLIMLRVTVHD 249

Query: 248 DSDTNARPLQPIYDRKTQLQRP 264
            S++NARPLQ   +R   L  P
Sbjct: 250 QSNSNARPLQRKNERPVALYIP 270

BLAST of CmaCh13G002450 vs. TAIR 10
Match: AT2G28210.1 (alpha carbonic anhydrase 2 )

HSP 1 Score: 253.1 bits (645), Expect = 2.6e-67
Identity = 112/204 (54.90%), Postives = 155/204 (75.98%), Query Frame = 0

Query: 24  VEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQSPIDMLHERVEVISHLGDLKINYK 83
           VEDE  F YE +   GP  WG+LRPEW  C  G MQSPID++++RV +++HL  L  +YK
Sbjct: 5   VEDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPIDLMNKRVRLVTHLKKLTRHYK 64

Query: 84  PSNATLKNRGHDMMMKW-IEGGGFMEVNGMQYKLKQCHWHSPSEHTIDGKKFDLEAHLVH 143
           P NATLKNRGHDMM+K+  EG G + VNG +YKL Q HWHSPSEHT++G++F LE H+VH
Sbjct: 65  PCNATLKNRGHDMMLKFGEEGSGSITVNGTEYKLLQLHWHSPSEHTMNGRRFALELHMVH 124

Query: 144 ESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHEEERIIGVIDPTLIKIGSKRYYR 203
           E+ NG ++V+ +LY+IG PD FL  +++ L  ++D +E E+ + VIDP  IKIGS+++YR
Sbjct: 125 ENINGSLAVVTVLYKIGRPDSFLGLLENKLSAITDQNEAEKYVDVIDPRDIKIGSRKFYR 184

Query: 204 YMGSLTVPPCTESVMWTMIHEVRT 227
           Y+GSLT PPCT++V+WT++ +V T
Sbjct: 185 YIGSLTTPPCTQNVIWTVVKKVNT 208

BLAST of CmaCh13G002450 vs. TAIR 10
Match: AT4G20990.1 (alpha carbonic anhydrase 4 )

HSP 1 Score: 238.4 bits (607), Expect = 6.5e-63
Identity = 115/256 (44.92%), Postives = 163/256 (63.67%), Query Frame = 0

Query: 11  CFLLLSFKAQS---QEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQSPIDMLHE 70
           CF+ LSF   S    EV+DE  F YE    KGP  WG++ P W  C  G  QSPID+ +E
Sbjct: 14  CFIYLSFPNISHAHSEVDDETPFTYEQKTEKGPEGWGKINPHWKVCNTGRYQSPIDLTNE 73

Query: 71  RVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCHWHSPSEH 130
           RV +I H       YKP+ A + NRGHD+M+ W    G M +    + L QCHWHSPSEH
Sbjct: 74  RVSLI-HDQAWTRQYKPAPAVITNRGHDIMVSWKGDAGKMTIRKTDFNLVQCHWHSPSEH 133

Query: 131 TIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHEEERIIGV 190
           T++G ++DLE H+VH S+ G  +VIG+LY++GEP+ FL+ + + ++ V +   +E  +G+
Sbjct: 134 TVNGTRYDLELHMVHTSARGRTAVIGVLYKLGEPNEFLTKLLNGIKAVGN---KEINLGM 193

Query: 191 IDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVHDDSDTNA 250
           IDP  I+  ++++YRY+GSLTVPPCTE V+WT++  V T++ EQ+  LR AV D  +TN+
Sbjct: 194 IDPREIRFQTRKFYRYIGSLTVPPCTEGVIWTVVKRVNTISMEQITALRQAVDDGFETNS 253

Query: 251 RPLQPIYDRKTQLQRP 264
           RP+Q    R      P
Sbjct: 254 RPVQDSKGRSVWFYDP 265

BLAST of CmaCh13G002450 vs. TAIR 10
Match: AT4G21000.1 (alpha carbonic anhydrase 6 )

HSP 1 Score: 208.0 bits (528), Expect = 9.5e-54
Identity = 96/218 (44.04%), Postives = 145/218 (66.51%), Query Frame = 0

Query: 8   FFFCFLLLSFK-----AQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQSPI 67
           FF  F +  F        ++E+ ++  F Y+    KGP  WG+L P+W  C  G +QSPI
Sbjct: 10  FFVVFFIDLFSPNILFVYAREIGNKPLFTYKQKTEKGPAEWGKLDPQWKVCSTGKIQSPI 69

Query: 68  DMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCHWH 127
           D+  ERV +I H   L  +YKP++A +++RGHD+M+ W   GG + ++   YKL QCHWH
Sbjct: 70  DLTDERVSLI-HDQALSKHYKPASAVIQSRGHDVMVSWKGDGGKITIHQTDYKLVQCHWH 129

Query: 128 SPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHEEE 187
           SPSEHTI+G  +DLE H+VH S++G  +V+G+LY++GEPD FL+ I + ++ V    ++E
Sbjct: 130 SPSEHTINGTSYDLELHMVHTSASGKTTVVGVLYKLGEPDEFLTKILNGIKGVG---KKE 189

Query: 188 RIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTM 221
             +G++DP  I+  +  +YRY+GSLT+PPCTE V+WT+
Sbjct: 190 IDLGIVDPRDIRFETNNFYRYIGSLTIPPCTEGVIWTV 223

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L8171.5e-8354.44Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1[more]
Q84UV81.4e-7354.58Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS... [more]
F4HUC49.8e-7249.62Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1[more]
Q9FM998.3e-7152.02Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3[more]
F4IHR43.3e-6752.68Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana OX=3702 GN=ACA2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1I0A87.4e-155100.00Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111469326 PE=3 SV=1[more]
A0A6J1HPL06.9e-15398.51Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111464882 PE=3 SV=1[more]
A0A6J1DHJ21.2e-13383.90Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111020525 PE=3 SV=1[more]
A0A6J1DG522.3e-13283.52Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111020526 PE=3 SV=1[more]
A0A1S4E3N87.0e-12183.54Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103502435 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022970331.11.5e-154100.00alpha carbonic anhydrase 7-like [Cucurbita maxima][more]
KAG6583572.12.2e-15398.88Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023520292.11.1e-15298.51alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo][more]
XP_022964924.11.4e-15298.51alpha carbonic anhydrase 7-like [Cucurbita moschata][more]
XP_038895739.11.9e-13385.39alpha carbonic anhydrase 7-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G08080.11.0e-8454.44alpha carbonic anhydrase 7 [more]
AT1G08065.17.0e-7349.62alpha carbonic anhydrase 5 [more]
AT2G28210.12.6e-6754.90alpha carbonic anhydrase 2 [more]
AT4G20990.16.5e-6344.92alpha carbonic anhydrase 4 [more]
AT4G21000.19.5e-5444.04alpha carbonic anhydrase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001148Alpha carbonic anhydrase domainSMARTSM01057Carb_anhydrase_2acoord: 32..259
e-value: 1.8E-65
score: 233.5
IPR001148Alpha carbonic anhydrase domainPFAMPF00194Carb_anhydrasecoord: 31..257
e-value: 6.8E-41
score: 140.5
IPR001148Alpha carbonic anhydrase domainPROSITEPS51144ALPHA_CA_2coord: 28..262
score: 45.23288
IPR036398Alpha carbonic anhydrase domain superfamilyGENE3D3.10.200.10Alpha carbonic anhydrasecoord: 26..266
e-value: 2.6E-73
score: 248.5
IPR036398Alpha carbonic anhydrase domain superfamilySUPERFAMILY51069Carbonic anhydrasecoord: 30..257
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 246..268
NoneNo IPR availablePANTHERPTHR18952:SF239BIFUNCTIONAL MONODEHYDROASCORBATE REDUCTASE AND CARBONIC ANHYDRASE NECTARIN-3coord: 19..265
IPR023561Carbonic anhydrase, alpha-classPANTHERPTHR18952CARBONIC ANHYDRASEcoord: 19..265
IPR018338Carbonic anhydrase, alpha-class, conserved sitePROSITEPS00162ALPHA_CA_1coord: 125..141
IPR041891Carbonic anhydrase, prokaryotic-likeCDDcd03124alpha_CA_prokaryotic_likecoord: 39..257
e-value: 4.67733E-95
score: 276.845

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh13G002450.1CmaCh13G002450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006730 one-carbon metabolic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0004089 carbonate dehydratase activity
molecular_function GO:0008270 zinc ion binding