CmaCh13G002060 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh13G002060
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionMajor facilitator superfamily protein
LocationCma_Chr13: 1630082 .. 1631998 (+)
RNA-Seq ExpressionCmaCh13G002060
SyntenyCmaCh13G002060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGTATCAGCAACGCCGAGCTCCAATGCCGGAATCGGGAGTGGAGAGCTTGGCAGTTTTGTCCGACAAGTGGTGGCGGGGCGGTGGTTTTCTCTTTTCGCTTCGTTTCTTGTTATGGCCGGCGCCGGCGGAGTTTATTTGTTTGCTTATTACTCTACAGACATCAAAACGACGCTCCAATGCGACCAAACGACGCTCAACAAGATTGGGTTTTACAAAGACCTGGGATCCAATGTTGGCATTGTCGCCGGCCTTCTGGCGGAGGTGGCGCCGACTTGGGTCCTTCTCCTCATCGGCGCCGCCTTGAACTTCGTCGGCTATTTCAAGATATGGCAAGGCGTCACCGGGAAAATTGTCAGCCCCACCGTCGCGTATTTTTGCTTTTACATTATGGTCGGAGCAAACTCTCAGAATTTCGCCAATACAGGTAATGCCTATGATTTAATATGAAAATATTAAACAGTTTAAAATCTTCAAAAACCCTACTTTAAAAAATTAAAGTGAAGGGTATTAATGAAAATTTTACATTTAAAATATCATTTACACTTTTAAAAATTTAAATACATTATTTAAGAGAGAGAGTATAAAAATTCAACGGTATTTGTTTCTTTTGTTGAAACTGCAACAGGCGTGCTAGTCACGTGCGTGAAGAACTTCCCGGAAAGACGAGGGGTAATGCTGGGGCTTGCGAAGGGGTTCGTGGGTCTAAGCGGAGCAATCATGACCCAAATTTACACCGCCATTTACGGCGACGATACCAGGTCGCTAATTCTGCTACTCGGCTGGCTCCCTTCTTTAATCTCTTTGATTTTCATCTCCACCATCAGAGAAATCAAAGCCGTCAAACACCCAAACGAGTTCAGAGTCTTCGTCCACTTCCTCTGCGTCTCCGTCATTCTCGCTCTGTTTCTCGCCGCCTTGATCTTCATCCAGAAACGAATCCGATTCGACCAATCCGCCCACGCCGCCGTCGTCGCCGCCATCGCGGCTCTGCTTCTTCTCCCCTTGTTAATCGCCATTAGAGAAGAAATCGTTCTATGGAATCTCAACAAACGGACGATCGGGATCCACCCGCCGCAAGCATCCCCCAATCCTCCTCCCTCTTCCTCCAACAAACCCTCAAGAGGAGAAGACCACACCATTCCTCAAGCCATTTTCAGCGCCGATATGCTCATCTTATGCATCACCATGCTAATCGGCGTCGGCGCTAGCTTGACCGCCATCGACAATCTCCGCCAGATCGGTGAATCCCATGGCTACCCATCTGAAACAATCAACTCGCTCATCGTTCTGGTTAGTATTTTCAACTTCACCGGAAGAATCTTTTCTGGGTTTGTCTCTGACATTCTACTCGAGAAATTCAAATTCCCAAGGCCATTAATGCTCACGCTTGTCCTCTTGGTCTCCTGCGTCGGCCATCTTTTGGTAGCCTTCCCCTTCCATGGTTCGCTGTACATTGCATCCATTGTTATTGGGTTTTCATTGGGCGCTCAAGTCCCATTGCATTTCGCCATGATTTCTGAGCTTTTTGGGTTGAAACATTACTCCACCTTGTTTAATTTTGGGCAATTGTCTTGCCCAATTGGGTCTTATATTCTGAATGTGATGGTCACTGGGAGGCTCTATGATGAGATGGCGAAGGCCACCAGTGGTATAGGCGGCCTTCATTGCGAAGGCCATTCTTGTTATGAACAGTCGTTCGTAATTTTAGCTGGGTTGACGTTCTTTGTAGCGTTGGTGTCGTTGATTTTGGTGGGGAGGACGAGGGAGTTTTATAGAGGGGATATATACAAGAAGTTTAGGGAAGATATGGAGTCTTTGAAGACGAAAATGGAGTTTTATACTCTCGATGACAAGAGGACTAGGATTGGGAATTTACTTGTTGACAAACACAGCATTAATTTCAAGTCGTGA

mRNA sequence

ATGATTGTATCAGCAACGCCGAGCTCCAATGCCGGAATCGGGAGTGGAGAGCTTGGCAGTTTTGTCCGACAAGTGGTGGCGGGGCGGTGGTTTTCTCTTTTCGCTTCGTTTCTTGTTATGGCCGGCGCCGGCGGAGTTTATTTGTTTGCTTATTACTCTACAGACATCAAAACGACGCTCCAATGCGACCAAACGACGCTCAACAAGATTGGGTTTTACAAAGACCTGGGATCCAATGTTGGCATTGTCGCCGGCCTTCTGGCGGAGGTGGCGCCGACTTGGGTCCTTCTCCTCATCGGCGCCGCCTTGAACTTCGTCGGCTATTTCAAGATATGGCAAGGCGTCACCGGGAAAATTGTCAGCCCCACCGTCGCGTATTTTTGCTTTTACATTATGGTCGGAGCAAACTCTCAGAATTTCGCCAATACAGGCGTGCTAGTCACGTGCGTGAAGAACTTCCCGGAAAGACGAGGGGTAATGCTGGGGCTTGCGAAGGGGTTCGTGGGTCTAAGCGGAGCAATCATGACCCAAATTTACACCGCCATTTACGGCGACGATACCAGGTCGCTAATTCTGCTACTCGGCTGGCTCCCTTCTTTAATCTCTTTGATTTTCATCTCCACCATCAGAGAAATCAAAGCCGTCAAACACCCAAACGAGTTCAGAGTCTTCGTCCACTTCCTCTGCGTCTCCGTCATTCTCGCTCTGTTTCTCGCCGCCTTGATCTTCATCCAGAAACGAATCCGATTCGACCAATCCGCCCACGCCGCCGTCGTCGCCGCCATCGCGGCTCTGCTTCTTCTCCCCTTGTTAATCGCCATTAGAGAAGAAATCGTTCTATGGAATCTCAACAAACGGACGATCGGGATCCACCCGCCGCAAGCATCCCCCAATCCTCCTCCCTCTTCCTCCAACAAACCCTCAAGAGGAGAAGACCACACCATTCCTCAAGCCATTTTCAGCGCCGATATGCTCATCTTATGCATCACCATGCTAATCGGCGTCGGCGCTAGCTTGACCGCCATCGACAATCTCCGCCAGATCGGTGAATCCCATGGCTACCCATCTGAAACAATCAACTCGCTCATCGTTCTGGTTAGTATTTTCAACTTCACCGGAAGAATCTTTTCTGGGTTTGTCTCTGACATTCTACTCGAGAAATTCAAATTCCCAAGGCCATTAATGCTCACGCTTGTCCTCTTGGTCTCCTGCGTCGGCCATCTTTTGGTAGCCTTCCCCTTCCATGGTTCGCTGTACATTGCATCCATTGTTATTGGGTTTTCATTGGGCGCTCAAGTCCCATTGCATTTCGCCATGATTTCTGAGCTTTTTGGGTTGAAACATTACTCCACCTTGTTTAATTTTGGGCAATTGTCTTGCCCAATTGGGTCTTATATTCTGAATGTGATGGTCACTGGGAGGCTCTATGATGAGATGGCGAAGGCCACCAGTGGTATAGGCGGCCTTCATTGCGAAGGCCATTCTTGTTATGAACAGTCGTTCGTAATTTTAGCTGGGTTGACGTTCTTTGTAGCGTTGGTGTCGTTGATTTTGGTGGGGAGGACGAGGGAGTTTTATAGAGGGGATATATACAAGAAGTTTAGGGAAGATATGGAGTCTTTGAAGACGAAAATGGAGTTTTATACTCTCGATGACAAGAGGACTAGGATTGGGAATTTACTTGTTGACAAACACAGCATTAATTTCAAGTCGTGA

Coding sequence (CDS)

ATGATTGTATCAGCAACGCCGAGCTCCAATGCCGGAATCGGGAGTGGAGAGCTTGGCAGTTTTGTCCGACAAGTGGTGGCGGGGCGGTGGTTTTCTCTTTTCGCTTCGTTTCTTGTTATGGCCGGCGCCGGCGGAGTTTATTTGTTTGCTTATTACTCTACAGACATCAAAACGACGCTCCAATGCGACCAAACGACGCTCAACAAGATTGGGTTTTACAAAGACCTGGGATCCAATGTTGGCATTGTCGCCGGCCTTCTGGCGGAGGTGGCGCCGACTTGGGTCCTTCTCCTCATCGGCGCCGCCTTGAACTTCGTCGGCTATTTCAAGATATGGCAAGGCGTCACCGGGAAAATTGTCAGCCCCACCGTCGCGTATTTTTGCTTTTACATTATGGTCGGAGCAAACTCTCAGAATTTCGCCAATACAGGCGTGCTAGTCACGTGCGTGAAGAACTTCCCGGAAAGACGAGGGGTAATGCTGGGGCTTGCGAAGGGGTTCGTGGGTCTAAGCGGAGCAATCATGACCCAAATTTACACCGCCATTTACGGCGACGATACCAGGTCGCTAATTCTGCTACTCGGCTGGCTCCCTTCTTTAATCTCTTTGATTTTCATCTCCACCATCAGAGAAATCAAAGCCGTCAAACACCCAAACGAGTTCAGAGTCTTCGTCCACTTCCTCTGCGTCTCCGTCATTCTCGCTCTGTTTCTCGCCGCCTTGATCTTCATCCAGAAACGAATCCGATTCGACCAATCCGCCCACGCCGCCGTCGTCGCCGCCATCGCGGCTCTGCTTCTTCTCCCCTTGTTAATCGCCATTAGAGAAGAAATCGTTCTATGGAATCTCAACAAACGGACGATCGGGATCCACCCGCCGCAAGCATCCCCCAATCCTCCTCCCTCTTCCTCCAACAAACCCTCAAGAGGAGAAGACCACACCATTCCTCAAGCCATTTTCAGCGCCGATATGCTCATCTTATGCATCACCATGCTAATCGGCGTCGGCGCTAGCTTGACCGCCATCGACAATCTCCGCCAGATCGGTGAATCCCATGGCTACCCATCTGAAACAATCAACTCGCTCATCGTTCTGGTTAGTATTTTCAACTTCACCGGAAGAATCTTTTCTGGGTTTGTCTCTGACATTCTACTCGAGAAATTCAAATTCCCAAGGCCATTAATGCTCACGCTTGTCCTCTTGGTCTCCTGCGTCGGCCATCTTTTGGTAGCCTTCCCCTTCCATGGTTCGCTGTACATTGCATCCATTGTTATTGGGTTTTCATTGGGCGCTCAAGTCCCATTGCATTTCGCCATGATTTCTGAGCTTTTTGGGTTGAAACATTACTCCACCTTGTTTAATTTTGGGCAATTGTCTTGCCCAATTGGGTCTTATATTCTGAATGTGATGGTCACTGGGAGGCTCTATGATGAGATGGCGAAGGCCACCAGTGGTATAGGCGGCCTTCATTGCGAAGGCCATTCTTGTTATGAACAGTCGTTCGTAATTTTAGCTGGGTTGACGTTCTTTGTAGCGTTGGTGTCGTTGATTTTGGTGGGGAGGACGAGGGAGTTTTATAGAGGGGATATATACAAGAAGTTTAGGGAAGATATGGAGTCTTTGAAGACGAAAATGGAGTTTTATACTCTCGATGACAAGAGGACTAGGATTGGGAATTTACTTGTTGACAAACACAGCATTAATTTCAAGTCGTGA

Protein sequence

MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTLQCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIVSPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYTAIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAALIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPPPSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETINSLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCVGHLLVAFPFHGSLYIASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMAKATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKKFREDMESLKTKMEFYTLDDKRTRIGNLLVDKHSINFKS
Homology
BLAST of CmaCh13G002060 vs. ExPASy Swiss-Prot
Match: Q03795 (Uncharacterized membrane protein YMR155W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YMR155W PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.1e-06
Identity = 124/544 (22.79%), Postives = 220/544 (40.44%), Query Frame = 0

Query: 28  GRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTLQCDQTTLNKIGFYKDLGSN-VGIVAGL 87
           G   S     +V  GAG  YLF++Y+  + +      +  +K+ F   +GS+ +GI+AG+
Sbjct: 17  GHLKSFVGGNVVALGAGTPYLFSFYAPQLLSKCHIPVSASSKLSFSLTIGSSLMGILAGI 76

Query: 88  LAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIVSPTVAYFCFYIMVGANSQNFANTGVL 147
           + + +P  +  LIG+   F+ Y  I         S T       +++G  S   +     
Sbjct: 77  VVDRSPK-LSCLIGSMCVFIAYL-ILNLCYKHEWSSTFLISLSLVLIGYGS--VSGFYAS 136

Query: 148 VTCVK-NFPERRGVMLGLAKGFVGLSGAIMTQIYTAIYGDDTRSLILLL------GWLPS 207
           V C   NFP+ RG          GLSG + + + + ++G++   + + L        L  
Sbjct: 137 VKCANTNFPQHRGTAGAFPVSLYGLSGMVFSYLCSKLFGENIEHVFIFLMVACGCMILVG 196

Query: 208 LISL-IFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAALIFIQKRIRFDQSAHAAV 267
             SL IF +   +  ++K   E+ +         I+ L+  +  +I   +R    A    
Sbjct: 197 YFSLDIFSNAEGDDASIK---EWELQKSRETDDNIVPLYENSNDYIGSPVRSSSPATYET 256

Query: 268 VA---------AIAAL---------LLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPP 327
            A            AL         LL P     + +    N +K T+G +  Q S    
Sbjct: 257 YALSDNFQETSEFFALEDRQLSNRPLLSPSSPHTKYDFEDENTSKNTVGENSAQKSMRLH 316

Query: 328 PSSSNKPSRGEDHTIPQAIFSAD--MLILCITMLIGVGASLTAIDNLRQIGESHGYPSET 387
              S K S    + I   I      M I  +  ++    S   ++ L          +E 
Sbjct: 317 VFQSLKSSTFIGYYIVLGILQGVGLMYIYSVGFMVQAQVSTPPLNQL-------PINAEK 376

Query: 388 INSL-IVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCVGHLLVAFPFHG- 447
           I SL + L+S+ +F GR+ SG +SD L++KFK  R   + +  L+  +    ++  F   
Sbjct: 377 IQSLQVTLLSLLSFCGRLSSGPISDFLVKKFKAQRLWNIVIASLLVFLASNKISHDFSSI 436

Query: 448 ------------SLYIASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGS 507
                       ++ + S + G+S G       +++++ FG   YSTL  +G L+     
Sbjct: 437 EDPSLRASKSFKNISVCSAIFGYSFGVLFGTFPSIVADRFGTNGYSTL--WGVLTT---G 496

Query: 508 YILNVMVTGRLYDEMAKATSGIGGLHC-EGHSCYEQSFVILAGLTFFVALVSLILVGRTR 528
            + +V V   +     KA +G    +C +G  CY  +F++      F  L  L ++G T 
Sbjct: 497 GVFSVSVFTDILGRDFKANTGDDDGNCKKGVLCYSYTFMVTKYCAAFNLLFVLGIIGYT- 539

BLAST of CmaCh13G002060 vs. ExPASy TrEMBL
Match: A0A6J1I5D1 (uncharacterized protein LOC111469394 OS=Cucurbita maxima OX=3661 GN=LOC111469394 PE=4 SV=1)

HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 571/571 (100.00%), Postives = 571/571 (100.00%), Query Frame = 0

Query: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL 60
           MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL
Sbjct: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL 60

Query: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120
           QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV
Sbjct: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120

Query: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180
           SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT
Sbjct: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180

Query: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240
           AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA
Sbjct: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240

Query: 241 LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPP 300
           LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPP
Sbjct: 241 LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPP 300

Query: 301 PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN 360
           PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN
Sbjct: 301 PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN 360

Query: 361 SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCVGHLLVAFPFHGSLYI 420
           SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCVGHLLVAFPFHGSLYI
Sbjct: 361 SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCVGHLLVAFPFHGSLYI 420

Query: 421 ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMA 480
           ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMA
Sbjct: 421 ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMA 480

Query: 481 KATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKKFREDMES 540
           KATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKKFREDMES
Sbjct: 481 KATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKKFREDMES 540

Query: 541 LKTKMEFYTLDDKRTRIGNLLVDKHSINFKS 572
           LKTKMEFYTLDDKRTRIGNLLVDKHSINFKS
Sbjct: 541 LKTKMEFYTLDDKRTRIGNLLVDKHSINFKS 571

BLAST of CmaCh13G002060 vs. ExPASy TrEMBL
Match: A0A6J1HYZ1 (uncharacterized protein LOC111469392 OS=Cucurbita maxima OX=3661 GN=LOC111469392 PE=4 SV=1)

HSP 1 Score: 1104.4 bits (2855), Expect = 0.0e+00
Identity = 570/571 (99.82%), Postives = 571/571 (100.00%), Query Frame = 0

Query: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL 60
           MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL
Sbjct: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL 60

Query: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120
           QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV
Sbjct: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120

Query: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180
           SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT
Sbjct: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180

Query: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240
           AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA
Sbjct: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240

Query: 241 LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPP 300
           LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPP
Sbjct: 241 LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPP 300

Query: 301 PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN 360
           PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN
Sbjct: 301 PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN 360

Query: 361 SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCVGHLLVAFPFHGSLYI 420
           SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSC+GHLLVAFPFHGSLYI
Sbjct: 361 SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCIGHLLVAFPFHGSLYI 420

Query: 421 ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMA 480
           ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMA
Sbjct: 421 ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMA 480

Query: 481 KATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKKFREDMES 540
           KATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKKFREDMES
Sbjct: 481 KATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKKFREDMES 540

Query: 541 LKTKMEFYTLDDKRTRIGNLLVDKHSINFKS 572
           LKTKMEFYTLDDKRTRIGNLLVDKHSINFKS
Sbjct: 541 LKTKMEFYTLDDKRTRIGNLLVDKHSINFKS 571

BLAST of CmaCh13G002060 vs. ExPASy TrEMBL
Match: A0A6J1HLR3 (uncharacterized protein LOC111464761 OS=Cucurbita moschata OX=3662 GN=LOC111464761 PE=4 SV=1)

HSP 1 Score: 1070.5 bits (2767), Expect = 2.5e-309
Identity = 555/571 (97.20%), Postives = 562/571 (98.42%), Query Frame = 0

Query: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL 60
           MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYS DIKTTL
Sbjct: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSRDIKTTL 60

Query: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120
           QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV
Sbjct: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120

Query: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180
           SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT
Sbjct: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180

Query: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240
           AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA
Sbjct: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240

Query: 241 LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPP 300
           L+FIQKRIRFDQSAHAAVVAAIA+LLLLPLLIAIREEIVLWNLNKRTIGIH PQ SPN P
Sbjct: 241 LVFIQKRIRFDQSAHAAVVAAIASLLLLPLLIAIREEIVLWNLNKRTIGIHQPQTSPN-P 300

Query: 301 PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN 360
           PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN
Sbjct: 301 PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN 360

Query: 361 SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCVGHLLVAFPFHGSLYI 420
           SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSC+GHLLVAFPF+ SLYI
Sbjct: 361 SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCIGHLLVAFPFNDSLYI 420

Query: 421 ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMA 480
           ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQL CPIGSYILNVMVTGRLYDEMA
Sbjct: 421 ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLFCPIGSYILNVMVTGRLYDEMA 480

Query: 481 KATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKKFREDMES 540
           K T+GIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILV RTREFY+GDIYKKFREDMES
Sbjct: 481 KTTTGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVERTREFYKGDIYKKFREDMES 540

Query: 541 LKTKMEFYTLDDKRTRIGNLLVDKHSINFKS 572
           LKTKMEFYTLD+KRTRIGNLLVDKHSIN KS
Sbjct: 541 LKTKMEFYTLDEKRTRIGNLLVDKHSINIKS 570

BLAST of CmaCh13G002060 vs. ExPASy TrEMBL
Match: A0A1S3CMG9 (uncharacterized protein LOC103502605 OS=Cucumis melo OX=3656 GN=LOC103502605 PE=4 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 8.7e-254
Identity = 457/595 (76.81%), Postives = 511/595 (85.88%), Query Frame = 0

Query: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL 60
           MIV+A   SN GI SGEL  F+ QVV GRWFSLFASFLVM GAGGVYLFAYYS DIKTTL
Sbjct: 1   MIVAAGQRSNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTL 60

Query: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120
           QCDQTTLN+IGFYKDLGSNVG++AGLLAEVAPTW++LLIGAA NF+GYFKIWQ VTGKIV
Sbjct: 61  QCDQTTLNRIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIV 120

Query: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180
            PTVA+FCFYIM+GANSQNFANTGVLVTCVKNFPERRGVMLGL KGFVGLSGAIMTQ+Y 
Sbjct: 121 RPTVAFFCFYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180

Query: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240
           AIYG DT+SLILL+ W PS++SLIF+ TIREIK VKHPNEFRVFV FLCV+V+LAL L A
Sbjct: 181 AIYGHDTKSLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTA 240

Query: 241 LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRT--------IGIH- 300
           LIF+QKR+ FDQSAH A+VAAI ALL +PLLIAIREE+V+WNLNKRT        IGI  
Sbjct: 241 LIFVQKRVHFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIES 300

Query: 301 ----PPQASPNPP-------PSSS-----NKPSRGEDHTIPQAIFSADMLILCITMLIGV 360
               PP  SP+ P       P+S      NKP RGED+T+ QAIFS DMLI+C TMLIGV
Sbjct: 301 SQPKPPIHSPSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGV 360

Query: 361 GASLTAIDNLRQIGESHGYPSETINSLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLM 420
           GASLTAIDNL QIGE+ GYPSETIN  + L+SI NFTGRIFSGFVS+ILLEKF+FPRPLM
Sbjct: 361 GASLTAIDNLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPLM 420

Query: 421 LTLVLLVSCVGHLLVAFPFHGSLYIASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNF 480
           LTL+LL+S +GHLLVAFPF  SLY+ASI+IGFS+G+QVPLHFAMISE+FGLKHYSTLFNF
Sbjct: 421 LTLILLISSLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNF 480

Query: 481 GQLSCPIGSYILNVMVTGRLYDEMAKATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVS 540
           GQLSCPIGSYILNVMVTG+LYDE+A+  S     HC G  CYEQSF+ILAGLTF VA++S
Sbjct: 481 GQLSCPIGSYILNVMVTGKLYDEVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMIS 540

Query: 541 LILVGRTREFYRGDIYKKFREDMESLKTKMEFYTLDDKRTRIGNLLVDKHSINFK 571
           LILV RTREFYRGDIYKKF+EDME+LKT++EFY +D+KRTRIGNLLVDKHSINFK
Sbjct: 541 LILVQRTREFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFK 595

BLAST of CmaCh13G002060 vs. ExPASy TrEMBL
Match: A0A5D3BP48 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2042G00080 PE=4 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 2.8e-252
Identity = 454/593 (76.56%), Postives = 509/593 (85.83%), Query Frame = 0

Query: 3   VSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTLQC 62
           ++A   SN GI SGEL  F+ QVV GRWFSLFASFLVM GAGGVYLFAYYS DIKTTLQC
Sbjct: 28  LAAGQRSNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTLQC 87

Query: 63  DQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIVSP 122
           DQTTLN+IGFYKDLGSNVG++AGLLAEVAPTW++LLIGAA NF+GYFKIWQ VTGKIV P
Sbjct: 88  DQTTLNRIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIVRP 147

Query: 123 TVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYTAI 182
           TVA+FCFYIM+GANSQNFANTGVLVTCVKNFPERRGVMLGL KGFVGLSGAIMTQ+Y AI
Sbjct: 148 TVAFFCFYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAI 207

Query: 183 YGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAALI 242
           YG DT+SLILL+ W PS++SLIF+ TIREIK VKHPNEFRVFV FLCV+V+LAL L ALI
Sbjct: 208 YGHDTKSLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTALI 267

Query: 243 FIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRT--------IGIH--- 302
           F+QKR+ FDQSAH A+VAAI ALL +PLLIAIREE+V+WNLNKRT        IGI    
Sbjct: 268 FVQKRVHFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIESSQ 327

Query: 303 --PPQASPNPP-------PSSS-----NKPSRGEDHTIPQAIFSADMLILCITMLIGVGA 362
             PP  SP+ P       P+S      NKP RGED+T+ QAIFS DMLI+C TMLIGVGA
Sbjct: 328 PKPPIHSPSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGVGA 387

Query: 363 SLTAIDNLRQIGESHGYPSETINSLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLT 422
           SLTAIDNL QIGE+ GYPSETIN  + L+SI NFTGRIFSGFVS+ILLEKF+FPRPLMLT
Sbjct: 388 SLTAIDNLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPLMLT 447

Query: 423 LVLLVSCVGHLLVAFPFHGSLYIASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQ 482
           L+LL+S +GHLLVAFPF  SLY+ASI+IGFS+G+QVPLHFAMISE+FGLKHYSTLFNFGQ
Sbjct: 448 LILLISSLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNFGQ 507

Query: 483 LSCPIGSYILNVMVTGRLYDEMAKATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLI 542
           LSCPIGSYILNVMVTG+LYDE+A+  S     HC G  CYEQSF+ILAGLTF VA++SLI
Sbjct: 508 LSCPIGSYILNVMVTGKLYDEVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMISLI 567

Query: 543 LVGRTREFYRGDIYKKFREDMESLKTKMEFYTLDDKRTRIGNLLVDKHSINFK 571
           LV RTREFYRGDIYKKF+EDME+LKT++EFY +D+KRTRIGNLLVDKHSINFK
Sbjct: 568 LVQRTREFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFK 620

BLAST of CmaCh13G002060 vs. NCBI nr
Match: XP_022970394.1 (uncharacterized protein LOC111469394 [Cucurbita maxima])

HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 571/571 (100.00%), Postives = 571/571 (100.00%), Query Frame = 0

Query: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL 60
           MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL
Sbjct: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL 60

Query: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120
           QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV
Sbjct: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120

Query: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180
           SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT
Sbjct: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180

Query: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240
           AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA
Sbjct: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240

Query: 241 LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPP 300
           LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPP
Sbjct: 241 LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPP 300

Query: 301 PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN 360
           PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN
Sbjct: 301 PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN 360

Query: 361 SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCVGHLLVAFPFHGSLYI 420
           SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCVGHLLVAFPFHGSLYI
Sbjct: 361 SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCVGHLLVAFPFHGSLYI 420

Query: 421 ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMA 480
           ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMA
Sbjct: 421 ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMA 480

Query: 481 KATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKKFREDMES 540
           KATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKKFREDMES
Sbjct: 481 KATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKKFREDMES 540

Query: 541 LKTKMEFYTLDDKRTRIGNLLVDKHSINFKS 572
           LKTKMEFYTLDDKRTRIGNLLVDKHSINFKS
Sbjct: 541 LKTKMEFYTLDDKRTRIGNLLVDKHSINFKS 571

BLAST of CmaCh13G002060 vs. NCBI nr
Match: XP_022970392.1 (uncharacterized protein LOC111469392 [Cucurbita maxima])

HSP 1 Score: 1104.4 bits (2855), Expect = 0.0e+00
Identity = 570/571 (99.82%), Postives = 571/571 (100.00%), Query Frame = 0

Query: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL 60
           MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL
Sbjct: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL 60

Query: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120
           QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV
Sbjct: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120

Query: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180
           SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT
Sbjct: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180

Query: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240
           AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA
Sbjct: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240

Query: 241 LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPP 300
           LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPP
Sbjct: 241 LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPP 300

Query: 301 PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN 360
           PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN
Sbjct: 301 PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN 360

Query: 361 SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCVGHLLVAFPFHGSLYI 420
           SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSC+GHLLVAFPFHGSLYI
Sbjct: 361 SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCIGHLLVAFPFHGSLYI 420

Query: 421 ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMA 480
           ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMA
Sbjct: 421 ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMA 480

Query: 481 KATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKKFREDMES 540
           KATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKKFREDMES
Sbjct: 481 KATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKKFREDMES 540

Query: 541 LKTKMEFYTLDDKRTRIGNLLVDKHSINFKS 572
           LKTKMEFYTLDDKRTRIGNLLVDKHSINFKS
Sbjct: 541 LKTKMEFYTLDDKRTRIGNLLVDKHSINFKS 571

BLAST of CmaCh13G002060 vs. NCBI nr
Match: XP_022964760.1 (uncharacterized protein LOC111464761 [Cucurbita moschata])

HSP 1 Score: 1070.5 bits (2767), Expect = 5.3e-309
Identity = 555/571 (97.20%), Postives = 562/571 (98.42%), Query Frame = 0

Query: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL 60
           MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYS DIKTTL
Sbjct: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSRDIKTTL 60

Query: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120
           QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV
Sbjct: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120

Query: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180
           SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT
Sbjct: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180

Query: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240
           AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA
Sbjct: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240

Query: 241 LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPP 300
           L+FIQKRIRFDQSAHAAVVAAIA+LLLLPLLIAIREEIVLWNLNKRTIGIH PQ SPN P
Sbjct: 241 LVFIQKRIRFDQSAHAAVVAAIASLLLLPLLIAIREEIVLWNLNKRTIGIHQPQTSPN-P 300

Query: 301 PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN 360
           PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN
Sbjct: 301 PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN 360

Query: 361 SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCVGHLLVAFPFHGSLYI 420
           SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSC+GHLLVAFPF+ SLYI
Sbjct: 361 SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCIGHLLVAFPFNDSLYI 420

Query: 421 ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMA 480
           ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQL CPIGSYILNVMVTGRLYDEMA
Sbjct: 421 ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLFCPIGSYILNVMVTGRLYDEMA 480

Query: 481 KATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKKFREDMES 540
           K T+GIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILV RTREFY+GDIYKKFREDMES
Sbjct: 481 KTTTGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVERTREFYKGDIYKKFREDMES 540

Query: 541 LKTKMEFYTLDDKRTRIGNLLVDKHSINFKS 572
           LKTKMEFYTLD+KRTRIGNLLVDKHSIN KS
Sbjct: 541 LKTKMEFYTLDEKRTRIGNLLVDKHSINIKS 570

BLAST of CmaCh13G002060 vs. NCBI nr
Match: XP_023520961.1 (uncharacterized protein LOC111784522 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1069.3 bits (2764), Expect = 1.1e-308
Identity = 552/571 (96.67%), Postives = 561/571 (98.25%), Query Frame = 0

Query: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL 60
           MIVSATPSSNAGIGSGELGSFVRQ+VAGRWFSLFASFLVMAGAGGVYLFAYYS DIKTTL
Sbjct: 1   MIVSATPSSNAGIGSGELGSFVRQLVAGRWFSLFASFLVMAGAGGVYLFAYYSRDIKTTL 60

Query: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120
           QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV
Sbjct: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120

Query: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180
           +PTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQ+YT
Sbjct: 121 NPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQLYT 180

Query: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240
           AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA
Sbjct: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240

Query: 241 LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPP 300
           LIFIQKRIRFDQSAHAAVV AIA+LLLLPLLIAIREEIVLWNLNKRTIGIH PQ SPNPP
Sbjct: 241 LIFIQKRIRFDQSAHAAVVTAIASLLLLPLLIAIREEIVLWNLNKRTIGIHQPQTSPNPP 300

Query: 301 PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN 360
            SSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN
Sbjct: 301 SSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN 360

Query: 361 SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCVGHLLVAFPFHGSLYI 420
           SLIVLVSIFNF GRIFSGFVSDILLEKFKFPRPLMLTLVLLVSC+GHLLVAFPF+ SLYI
Sbjct: 361 SLIVLVSIFNFAGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCIGHLLVAFPFNDSLYI 420

Query: 421 ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMA 480
           ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQL CPIGSYILNVMVTGRLYDEMA
Sbjct: 421 ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLFCPIGSYILNVMVTGRLYDEMA 480

Query: 481 KATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKKFREDMES 540
           KA++GIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILV RTREFYRGDIYKKFREDMES
Sbjct: 481 KASAGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVERTREFYRGDIYKKFREDMES 540

Query: 541 LKTKMEFYTLDDKRTRIGNLLVDKHSINFKS 572
           LKTKMEFYTLD+KRTRIGNLLVDKHSIN KS
Sbjct: 541 LKTKMEFYTLDEKRTRIGNLLVDKHSINIKS 571

BLAST of CmaCh13G002060 vs. NCBI nr
Match: KAG6583514.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1069.3 bits (2764), Expect = 1.1e-308
Identity = 554/571 (97.02%), Postives = 562/571 (98.42%), Query Frame = 0

Query: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL 60
           MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYS DIKTTL
Sbjct: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSRDIKTTL 60

Query: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120
           QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV
Sbjct: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120

Query: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180
           +PTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT
Sbjct: 121 NPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180

Query: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240
           AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA
Sbjct: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240

Query: 241 LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPP 300
           L+FIQKRIRFDQSAHAAVVAAIA+LLLLPLLIAIREEIVLWNLNKRTIGIH PQ SPN P
Sbjct: 241 LVFIQKRIRFDQSAHAAVVAAIASLLLLPLLIAIREEIVLWNLNKRTIGIHQPQTSPN-P 300

Query: 301 PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN 360
           PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN
Sbjct: 301 PSSSNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETIN 360

Query: 361 SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCVGHLLVAFPFHGSLYI 420
           SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSC+GHLLVAFPF+ SLYI
Sbjct: 361 SLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCIGHLLVAFPFNDSLYI 420

Query: 421 ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMA 480
           ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQL CPIGSYILNVMVTGRLYDEMA
Sbjct: 421 ASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLFCPIGSYILNVMVTGRLYDEMA 480

Query: 481 KATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKKFREDMES 540
           K T+GIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILV RTREFY+GDIYKKFREDMES
Sbjct: 481 KTTTGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVERTREFYKGDIYKKFREDMES 540

Query: 541 LKTKMEFYTLDDKRTRIGNLLVDKHSINFKS 572
           LKTKMEFYTLD+KRTRIGNLLVDKHSIN KS
Sbjct: 541 LKTKMEFYTLDEKRTRIGNLLVDKHSINIKS 570

BLAST of CmaCh13G002060 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 590.9 bits (1522), Expect = 1.1e-168
Identity = 314/561 (55.97%), Postives = 395/561 (70.41%), Query Frame = 0

Query: 13  IGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTLQCDQTTLNKIGF 72
           +G+ E   F+     GRWF +FASFL+MA AG  YLF  YS DIK+TL  DQTTLN +GF
Sbjct: 1   MGNDETVRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGF 60

Query: 73  YKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIVSPTVAYFCFYIM 132
           +KDLG+NVG+++GL+AEV PTW +L IG+A+NFVGYF IW  VTGK+  P V   C YI 
Sbjct: 61  FKDLGANVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYIC 120

Query: 133 VGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYTAIYGDDTRSLIL 192
           +GANSQNFANTG LVTCVKNFPE RGVMLGL KG+VGLSGAI TQ+Y AIYG D++SLIL
Sbjct: 121 IGANSQNFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLIL 180

Query: 193 LLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAALIFIQKRIRFDQ 252
           L+ WLP+ +SL+F+  IRE K V+  NE  VF  FL +S+ LALFL A+   +K++ F +
Sbjct: 181 LIAWLPAAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSK 240

Query: 253 SAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPPPSS--------- 312
           +A+AA      ALL +PL +++++E+ +WN+ K  I   P +     P            
Sbjct: 241 AAYAASATICCALLFVPLTVSVKQELEVWNMMKLPIE-EPSEVKVEKPKKELDLDQDKAA 300

Query: 313 ----------------SNKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQ 372
                            + P RGED+TI QA+ S DM+IL +    G+G+SLTA+DNL Q
Sbjct: 301 KVNGEEKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQ 360

Query: 373 IGESHGYPSETINSLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCVGH 432
           IGES GYP+ T++S + LVSI+N+ GR+FSGFVS+ LL K+K PRPLM+TLVLL+SC GH
Sbjct: 361 IGESLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGH 420

Query: 433 LLVAFPFHGSLYIASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYIL 492
           LL+AFP  GS+YIASI++GFS GAQ+PL FA+ISELFGLK+YSTLFN GQL+ P+GSYIL
Sbjct: 421 LLIAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYIL 480

Query: 493 NVMVTGRLYDEMA--------KATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILV 541
           NV VTG LYD+ A             +  L C G  CY+  F+ILA +TFF ALVSL L 
Sbjct: 481 NVRVTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLA 540

BLAST of CmaCh13G002060 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 547.7 bits (1410), Expect = 1.1e-155
Identity = 296/570 (51.93%), Postives = 385/570 (67.54%), Query Frame = 0

Query: 16  GELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTLQCDQTTLNKIGFYKD 75
           G + S   Q++ GRWF  F S L+M+ AG  Y+F  YS DIK TL  DQTTLN + F+KD
Sbjct: 8   GSMKSLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKD 67

Query: 76  LGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIVSPTVAYFCFYIMVGA 135
           LG+NVG++AGLL EV P W +LLIGA LNF GYF IW  VT +I  P V + C YI VGA
Sbjct: 68  LGANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGA 127

Query: 136 NSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYTAIYGDDTRSLILLLG 195
           NSQ+FANTG LVTCVKNFPE RGV+LG+ KG+VGLSGAI+TQ+Y A YG+DT+ LIL++G
Sbjct: 128 NSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIG 187

Query: 196 WLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAALIFIQKRIRFDQSAH 255
           WLP+++S  F+ TIR +K  +  NE +VF +FL +S+ LA FL  +I I K   F QS  
Sbjct: 188 WLPAIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEF 247

Query: 256 AAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQ----ASPNPPPSSS------- 315
               A +  LLLLP+++ I EE  LW   ++ + ++ P      +  P   SS       
Sbjct: 248 GGSAAVVIVLLLLPIIVVILEEKKLW--KEKQVALNDPAPINVVTEKPKLDSSEFKDDDG 307

Query: 316 --------------------NKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDN 375
                               N P RG+D+TI QA+FS DMLIL +  + GVG +LTAIDN
Sbjct: 308 EESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDN 367

Query: 376 LRQIGESHGYPSETINSLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSC 435
           L QIG S GYP  ++++ + LVSI+N+ GR+ SG VS+I L K+KFPRPLMLT+VLL+SC
Sbjct: 368 LGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSC 427

Query: 436 VGHLLVAFPFHGSLYIASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGS 495
            GHLL+AF   G LY+AS++IGF  GAQ PL FA+ISE+FGLK+YSTL+NFG ++ PIGS
Sbjct: 428 AGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGS 487

Query: 496 YILNVMVTGRLYD-EMAKATSGIG-------GLHCEGHSCYEQSFVILAGLTFFVALVSL 547
           Y+LNV V G LYD E  K    +G        L+C G SC++ SF+I+A +T F  LVS+
Sbjct: 488 YLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSM 547

BLAST of CmaCh13G002060 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 310.1 bits (793), Expect = 3.8e-84
Identity = 190/535 (35.51%), Postives = 294/535 (54.95%), Query Frame = 0

Query: 24  QVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTLQCDQTTLNKIGFYKDLGSNVGIV 83
           +++  +W ++ AS  +   +G  Y F  YS  +K+T   DQ+TL+ +  +KD+G+N G+ 
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 84  AGLLAEVAPT----------------WVLLLIGAALNFVGYFKIWQGVTGKIVSPTVAYF 143
           +GLL   A +                WV+L +GA   F GYF IW  VTG I  P V   
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 144 CFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYTAIYGDDT 203
           C ++ + A SQ F NT  +V+ V+NF +  G  +G+ KGF+GLSGAI+ Q+Y  +   D 
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDP 181

Query: 204 RSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAALIFIQKR 263
            S ILLL   P+++SL+ +  +R I      ++ +       VS+I+A +L  +I ++  
Sbjct: 182 ASFILLLAVTPTVLSLLVMPLVR-IYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNT 241

Query: 264 IRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPPPSSSNKP 323
                 A+   +  +  +L LPLLIA R +     + K     + P  S     +S N+ 
Sbjct: 242 FGLSSWANIVTLVCLLVMLALPLLIARRAQ--RDGMEKTVPHDYSPLISSPKATTSGNQS 301

Query: 324 SRG---------EDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSET 383
           S G         E+  + QA+      +L + M+ G+G+ L+ I+N+RQIGES  Y S  
Sbjct: 302 SEGDSKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIGESLRYSSVE 361

Query: 384 INSLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCVGHLLVAFPFHGSL 443
           INSL+ L SI+NF GR  +G+ SD LL K  +PRPL++   L    +GHL++A  F G+L
Sbjct: 362 INSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGFQGNL 421

Query: 444 YIASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDE 503
           Y+ S+++G   G+Q  L   + SELFG++H  T+FN   ++ PIGSYI +V + G +YD 
Sbjct: 422 YVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSVRLIGYIYD- 481

Query: 504 MAKATSGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKK 534
             K  SG G   C G  C+  SF+I+A + FF  LV+++L  RT+  YR  + K+
Sbjct: 482 --KTASGEGNT-CYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLYRQILVKR 529

BLAST of CmaCh13G002060 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 310.1 bits (793), Expect = 3.8e-84
Identity = 188/527 (35.67%), Postives = 298/527 (56.55%), Query Frame = 0

Query: 24  QVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTLQCDQTTLNKIGFYKDLGSNVGIV 83
           +++  +W ++ AS  +   AGG Y F  YS  +K+T   DQ+TL+ +  +KD+G NVG++
Sbjct: 4   EILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVL 63

Query: 84  AGLLAEVAP--------------TWVLLLIGAALNFVGYFKIWQGVTGKIVSPTVAYFCF 143
           +GL+   A                WV++LIGA LNF GYF +W  VTG I  P V   C 
Sbjct: 64  SGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCL 123

Query: 144 YIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYTAIYGDDTRS 203
           ++ + A S  F NT  +V+ ++NF +  G  +G+ KGFVGLSGA++ Q+Y  +   D ++
Sbjct: 124 FMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDPKT 183

Query: 204 LILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAALIFIQKRIR 263
            ILLL  +PSL+S++ +  +R  K     +E +       +S+I+A +L   I ++  + 
Sbjct: 184 FILLLAIVPSLLSVLVMPLVRVYK-TSTVDEKKHLDGLSTLSLIIAAYLMITIILKSTLS 243

Query: 264 FDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPPPSSSNKPSR 323
               A+A  +A +  LL  PLL+A+R      ++ K    ++ P        +S      
Sbjct: 244 LPSWANAVTLAVLLVLLSSPLLVAVRAH--RDSIEKPLSSVYSPLVDNLEATTSGEILML 303

Query: 324 GEDHTIP--QAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETINSLIVLVS 383
            ED ++   QA+ + D  +L + M+ G+G+ ++ I+N+RQIGES  Y S  INSL+ L +
Sbjct: 304 DEDKSLNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQIGESLRYTSVEINSLLALWN 363

Query: 384 IFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCVGHLLVAFPFHGSLYIASIVIGF 443
           I+NF GR   G+VSD LL +  +PRPL++   L    +GHL++A  F G+LY  SI++G 
Sbjct: 364 IWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHLIIASGFQGNLYPGSIIVGI 423

Query: 444 SLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMAKATSGIG 503
             G+Q  L   + SELFG+KH  T++N   ++ P+GSYI +V + G +YD        IG
Sbjct: 424 CYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFSVRLIGYIYDRTI-----IG 483

Query: 504 -GLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKK 534
            G  C G  C+  ++V++A + F   LVS +LV RT+  YR  I++K
Sbjct: 484 EGNTCYGPHCFRLAYVVIASVAFLGFLVSCVLVFRTKTIYR-QIFEK 521

BLAST of CmaCh13G002060 vs. TAIR 10
Match: AT2G34350.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 285.8 bits (730), Expect = 7.6e-77
Identity = 176/526 (33.46%), Postives = 283/526 (53.80%), Query Frame = 0

Query: 29  RWFSLFASFLVMAGAGGVYLFAYYSTDIKTTLQCDQTTLNKIGFYKDLGSNVGIVAGLLA 88
           +W +  AS  + + +G  Y F  YS+ +K++   DQ+TL+ +  YKD+G+NVGI++GL  
Sbjct: 7   KWVAAAASIWIQSFSGASYTFGIYSSVLKSSQSYDQSTLDTVSVYKDIGANVGILSGLFY 66

Query: 89  EVAPT------------WVLLLIGAALNFVGYFKIWQGVTGKIVSPTVAYFCFYIMVGAN 148
               +            W+++ +G    FVGY  IW   +G I  P VA  C ++    +
Sbjct: 67  TAVASRKSGNGGFFSGPWLVIFVGLLQWFVGYGFIWMATSGVIPRPPVAMMCLFMFFAGH 126

Query: 149 SQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYTAIYGDDTRSLILLLGW 208
            Q F NT ++VT V+NF +  G  +G+ KG++GLSGAI+ Q+Y    G D R+ ILLL  
Sbjct: 127 CQPFFNTAIVVTAVRNFSDYGGTAVGIMKGYLGLSGAILVQMYHIFCGGDPRNYILLLAV 186

Query: 209 LPSLISLIFISTIREIKAV-----KHPNEFRVFVHFLCVSVILALFLAALIFIQKRIRFD 268
           +PSL+ L  +  +R    V     KH N          +S+I+  +L  +I ++  I   
Sbjct: 187 VPSLLILTLMPFVRTYDTVIAGDKKHLNGLS------AISLIIVTYLMVVILVENIIGMS 246

Query: 269 QSAHAAVVAAIAALLLLPLLIAIR----EEIVLWNLNKRTIGIHPPQASPNPPPSSSNKP 328
                     +  LL  PLL+A+R    EE    +L+           SP    SS  K 
Sbjct: 247 MPMKICSFTFLLLLLASPLLVAVRAQREEEHRFLSLDFPVTERTTLLDSPKLNSSSDVKD 306

Query: 329 SRGEDHTIPQAIFSADMLILCITMLIGVGASLTAIDNLRQIGESHGYPSETINSLIVLVS 388
               D  + +AI + +  +L + M+ G+G+ L  I+N+RQ+GES  Y +  +NSL+ L S
Sbjct: 307 VMTNDMNVLEAICTTNFWLLFVAMICGMGSGLATINNIRQMGESLRYSTVQLNSLVSLWS 366

Query: 389 IFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVLLVSCVGHLLVAFPFHGSLYIASIVIGF 448
           I+NF GR  SG++SD  L    +PRP+ + + L +  +GH+++A    GSLYI S+++G 
Sbjct: 367 IWNFLGRFGSGYISDTYLHSHGWPRPVFMAITLGLMAIGHIVMASGLLGSLYIGSLLVGL 426

Query: 449 SLGAQVPLHFAMISELFGLKHYSTLFNFGQLSCPIGSYILNVMVTGRLYDEMAKATSGIG 508
           + G+Q  L   + SE+FG+ H  T+F    ++ P+GSY  +V V G LYD++A       
Sbjct: 427 AYGSQWSLMPTITSEIFGVLHMGTIFYTISIASPVGSYFFSVKVIGYLYDKVASEDDH-- 486

Query: 509 GLHCEGHSCYEQSFVILAGLTFFVALVSLILVGRTREFYRGDIYKK 534
              C G+ C+  SF+I+A +    +LV+L+L+ RT++FY   + K+
Sbjct: 487 --SCYGNHCFRTSFLIMAAMALLGSLVALVLLLRTKKFYATLVAKR 522

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q037953.1e-0622.79Uncharacterized membrane protein YMR155W OS=Saccharomyces cerevisiae (strain ATC... [more]
Match NameE-valueIdentityDescription
A0A6J1I5D10.0e+00100.00uncharacterized protein LOC111469394 OS=Cucurbita maxima OX=3661 GN=LOC111469394... [more]
A0A6J1HYZ10.0e+0099.82uncharacterized protein LOC111469392 OS=Cucurbita maxima OX=3661 GN=LOC111469392... [more]
A0A6J1HLR32.5e-30997.20uncharacterized protein LOC111464761 OS=Cucurbita moschata OX=3662 GN=LOC1114647... [more]
A0A1S3CMG98.7e-25476.81uncharacterized protein LOC103502605 OS=Cucumis melo OX=3656 GN=LOC103502605 PE=... [more]
A0A5D3BP482.8e-25276.56Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
XP_022970394.10.0e+00100.00uncharacterized protein LOC111469394 [Cucurbita maxima][more]
XP_022970392.10.0e+0099.82uncharacterized protein LOC111469392 [Cucurbita maxima][more]
XP_022964760.15.3e-30997.20uncharacterized protein LOC111464761 [Cucurbita moschata][more]
XP_023520961.11.1e-30896.67uncharacterized protein LOC111784522 [Cucurbita pepo subsp. pepo][more]
KAG6583514.11.1e-30897.02Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
AT2G28120.11.1e-16855.97Major facilitator superfamily protein [more]
AT2G39210.11.1e-15551.93Major facilitator superfamily protein [more]
AT1G74780.13.8e-8435.51Nodulin-like / Major Facilitator Superfamily protein [more]
AT1G18940.13.8e-8435.67Nodulin-like / Major Facilitator Superfamily protein [more]
AT2G34350.17.6e-7733.46Nodulin-like / Major Facilitator Superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 531..551
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 291..311
NoneNo IPR availablePANTHERPTHR21576:SF29PROTEIN NUCLEAR FUSION DEFECTIVE 4coord: 14..542
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 14..542
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 327..522
e-value: 4.3E-11
score: 42.3
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 29..274
e-value: 2.5E-78
score: 263.0
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 320..526
e-value: 4.7E-12
score: 47.7
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 21..215
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 227..524

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh13G002060.1CmaCh13G002060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity