CmaCh13G002050 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh13G002050
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionMajor facilitator superfamily protein
LocationCma_Chr13: 1625521 .. 1627350 (-)
RNA-Seq ExpressionCmaCh13G002050
SyntenyCmaCh13G002050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGTATCATCAACAATACCCATCTGCCATGGCAGAATGGAGAGTGCACAATTCTCCAACTTCGCTCGCCAAGTGGTGGTGGGGCGATGGTTCTCTCTCTTTGCTTCCTTCCTTGTAATGACAAGCGCCGGTGCATTTTACTTTTATTCTTACTTTTCCGAACACATTAAAGAAACGCTCCAATGTGACCAAACAACGCTCAACAAGATTAGCTTCTACAAAGACATTGGATCCAACATCGACATTGTCTCTGCCCTCCTTGTTTGCATTGCGCCCTCTTGGGTCCTTCTCCTCATCTGCTCCGCCCTCAACTTCGTCGGCTACTTCAAGATATGGCAAGCCGTGGTCGGACAAGTTGTGCACCCAACCGTTGAGTACTTCGGCGTTTACATAGCAATGGCCGGAAACTCTCAGATTTTAGCCAATACAGGTGAGAATTGAATTGAGTTTCAAAATAATGTCTCCTAATTAGCTCATAATTCTTGCTTCGTTTCAACAGTGGTGCTAGTCATATGCGTGAGGAACTTCCCTGAAAGAAGAGGGGTGATATTGGGGCTTCTGAAGGGCTTCTTGGGCTTAGGAGCGGCTGTACTGACACAAGTATACCTCGCCATTTATGGTCATGATACCAAGTCTCTGATTCTGTTCATTGCGTGGTGCCCATCCTTGATTACTCTGCTTTTTGCCTTCTCAATCAGGGAAGTCAAAATTGTGAAACACCCACATGAGTTCAGAGTTTTCATTCAGATTCTATGCATCACCCTCGTTTTAGCTCTGTTTCTCACAGTGTTAACCATCGCTGAGCAAATGGTTCAATTCGATCAATTAGCCTACACTTTTGTGGTGGTTGCAATCATTGGTTTGCTTATTCTCCCATTGTTCATAGCCATTAGAGAAGAAATGGTTCAGCTGAATCTTAACAACATGACCAAATTTGAAAAATCCCGATCAAAACCTCAAATACCCATCCATGCAATTTCGCATAACCAAAATCATGGAAACTCCTATTATTCAAACATTTTCAACAAACCCGAAAGAGGAGATGATTACACAATTCTTCAAGGAATTTTCAGCATTGACATGCTCCTCATATGCCTTGCAATGTCAATTGGTACCGGCTCAAGCCTTACAGCCATGGACAACCTCGGCCAAATCGGAGAATCCCAAGGATACTCCACTGAATCCGTCAATGCAATGGTCTCCATCATCAGCATTTTTAACTTCCTTGGAAGAATCTTCTCTGGGTTTGTTTCAGAAATCCTGCTCGAGAAATACAAATTTCCCCGCCCGTTGATGCTGACTCTAACCCTTTTGGTGTCGTGCATTGGTTTCTTGTTGGTGGCCTTCCCCTTTCATAATTCGCTCTACATTGCATCAATCTTAATTGGGTTTGCTTTTGGGTCTCAAGTTCTATTGGATATCACCATGATTTCAGAGTTTTTTGGTCTTGAACATTACGCCATGTTTTATAATGTTGCGCAACTGGCTTGTCCAATTGGGACTTATGTTCTGAAGGTGTTAGTGGCTGGAAAGCTGTACGACGAGGAAGTGAAGACATGGAGTGATTCACCAGAGTATATATGCCATGGGGAACAATGTTACAGAAACTCTTTCACGTTTTTGACTGAGATGTCGCTGTTGGGAGCTGCAATATCATTGATTTTGGTGAAGAGAACGAGGGAGTTTTATAGACAGGATATATACAAGAAGTTTAGGGAAGATATGGATTCTTTGAAGGAGGAAGTGGAACTTTACCCTCACGATGTGCGGCAGCCTGAAGTTGAAAGCGTACATGTTCACAAAGAAGTTACCATTTTCAAGAAGTGA

mRNA sequence

ATGGTTGTATCATCAACAATACCCATCTGCCATGGCAGAATGGAGAGTGCACAATTCTCCAACTTCGCTCGCCAAGTGGTGGTGGGGCGATGGTTCTCTCTCTTTGCTTCCTTCCTTGTAATGACAAGCGCCGGTGCATTTTACTTTTATTCTTACTTTTCCGAACACATTAAAGAAACGCTCCAATGTGACCAAACAACGCTCAACAAGATTAGCTTCTACAAAGACATTGGATCCAACATCGACATTGTCTCTGCCCTCCTTGTTTGCATTGCGCCCTCTTGGGTCCTTCTCCTCATCTGCTCCGCCCTCAACTTCGTCGGCTACTTCAAGATATGGCAAGCCGTGGTCGGACAAGTTGTGCACCCAACCGTTGAGTACTTCGGCGTTTACATAGCAATGGCCGGAAACTCTCAGATTTTAGCCAATACAGTGGTGCTAGTCATATGCGTGAGGAACTTCCCTGAAAGAAGAGGGGTGATATTGGGGCTTCTGAAGGGCTTCTTGGGCTTAGGAGCGGCTGTACTGACACAAGTATACCTCGCCATTTATGGTCATGATACCAAGTCTCTGATTCTGTTCATTGCGTGGTGCCCATCCTTGATTACTCTGCTTTTTGCCTTCTCAATCAGGGAAGTCAAAATTGTGAAACACCCACATGAGTTCAGAGTTTTCATTCAGATTCTATGCATCACCCTCGTTTTAGCTCTGTTTCTCACAGTGTTAACCATCGCTGAGCAAATGGTTCAATTCGATCAATTAGCCTACACTTTTGTGGTGGTTGCAATCATTGGTTTGCTTATTCTCCCATTGTTCATAGCCATTAGAGAAGAAATGGTTCAGCTGAATCTTAACAACATGACCAAATTTGAAAAATCCCGATCAAAACCTCAAATACCCATCCATGCAATTTCGCATAACCAAAATCATGGAAACTCCTATTATTCAAACATTTTCAACAAACCCGAAAGAGGAGATGATTACACAATTCTTCAAGGAATTTTCAGCATTGACATGCTCCTCATATGCCTTGCAATGTCAATTGGTACCGGCTCAAGCCTTACAGCCATGGACAACCTCGGCCAAATCGGAGAATCCCAAGGATACTCCACTGAATCCGTCAATGCAATGGTCTCCATCATCAGCATTTTTAACTTCCTTGGAAGAATCTTCTCTGGGTTTGTTTCAGAAATCCTGCTCGAGAAATACAAATTTCCCCGCCCGTTGATGCTGACTCTAACCCTTTTGGTGTCGTGCATTGGTTTCTTGTTGGTGGCCTTCCCCTTTCATAATTCGCTCTACATTGCATCAATCTTAATTGGGTTTGCTTTTGGGTCTCAAGTTCTATTGGATATCACCATGATTTCAGAGTTTTTTGGTCTTGAACATTACGCCATGTTTTATAATGTTGCGCAACTGGCTTGTCCAATTGGGACTTATGTTCTGAAGGTGTTAGTGGCTGGAAAGCTGTACGACGAGGAAGTGAAGACATGGAGTGATTCACCAGAGTATATATGCCATGGGGAACAATGTTACAGAAACTCTTTCACGTTTTTGACTGAGATGTCGCTGTTGGGAGCTGCAATATCATTGATTTTGGTGAAGAGAACGAGGGAGTTTTATAGACAGGATATATACAAGAAGTTTAGGGAAGATATGGATTCTTTGAAGGAGGAAGTGGAACTTTACCCTCACGATGTGCGGCAGCCTGAAGTTGAAAGCGTACATGTTCACAAAGAAGTTACCATTTTCAAGAAGTGA

Coding sequence (CDS)

ATGGTTGTATCATCAACAATACCCATCTGCCATGGCAGAATGGAGAGTGCACAATTCTCCAACTTCGCTCGCCAAGTGGTGGTGGGGCGATGGTTCTCTCTCTTTGCTTCCTTCCTTGTAATGACAAGCGCCGGTGCATTTTACTTTTATTCTTACTTTTCCGAACACATTAAAGAAACGCTCCAATGTGACCAAACAACGCTCAACAAGATTAGCTTCTACAAAGACATTGGATCCAACATCGACATTGTCTCTGCCCTCCTTGTTTGCATTGCGCCCTCTTGGGTCCTTCTCCTCATCTGCTCCGCCCTCAACTTCGTCGGCTACTTCAAGATATGGCAAGCCGTGGTCGGACAAGTTGTGCACCCAACCGTTGAGTACTTCGGCGTTTACATAGCAATGGCCGGAAACTCTCAGATTTTAGCCAATACAGTGGTGCTAGTCATATGCGTGAGGAACTTCCCTGAAAGAAGAGGGGTGATATTGGGGCTTCTGAAGGGCTTCTTGGGCTTAGGAGCGGCTGTACTGACACAAGTATACCTCGCCATTTATGGTCATGATACCAAGTCTCTGATTCTGTTCATTGCGTGGTGCCCATCCTTGATTACTCTGCTTTTTGCCTTCTCAATCAGGGAAGTCAAAATTGTGAAACACCCACATGAGTTCAGAGTTTTCATTCAGATTCTATGCATCACCCTCGTTTTAGCTCTGTTTCTCACAGTGTTAACCATCGCTGAGCAAATGGTTCAATTCGATCAATTAGCCTACACTTTTGTGGTGGTTGCAATCATTGGTTTGCTTATTCTCCCATTGTTCATAGCCATTAGAGAAGAAATGGTTCAGCTGAATCTTAACAACATGACCAAATTTGAAAAATCCCGATCAAAACCTCAAATACCCATCCATGCAATTTCGCATAACCAAAATCATGGAAACTCCTATTATTCAAACATTTTCAACAAACCCGAAAGAGGAGATGATTACACAATTCTTCAAGGAATTTTCAGCATTGACATGCTCCTCATATGCCTTGCAATGTCAATTGGTACCGGCTCAAGCCTTACAGCCATGGACAACCTCGGCCAAATCGGAGAATCCCAAGGATACTCCACTGAATCCGTCAATGCAATGGTCTCCATCATCAGCATTTTTAACTTCCTTGGAAGAATCTTCTCTGGGTTTGTTTCAGAAATCCTGCTCGAGAAATACAAATTTCCCCGCCCGTTGATGCTGACTCTAACCCTTTTGGTGTCGTGCATTGGTTTCTTGTTGGTGGCCTTCCCCTTTCATAATTCGCTCTACATTGCATCAATCTTAATTGGGTTTGCTTTTGGGTCTCAAGTTCTATTGGATATCACCATGATTTCAGAGTTTTTTGGTCTTGAACATTACGCCATGTTTTATAATGTTGCGCAACTGGCTTGTCCAATTGGGACTTATGTTCTGAAGGTGTTAGTGGCTGGAAAGCTGTACGACGAGGAAGTGAAGACATGGAGTGATTCACCAGAGTATATATGCCATGGGGAACAATGTTACAGAAACTCTTTCACGTTTTTGACTGAGATGTCGCTGTTGGGAGCTGCAATATCATTGATTTTGGTGAAGAGAACGAGGGAGTTTTATAGACAGGATATATACAAGAAGTTTAGGGAAGATATGGATTCTTTGAAGGAGGAAGTGGAACTTTACCCTCACGATGTGCGGCAGCCTGAAGTTGAAAGCGTACATGTTCACAAAGAAGTTACCATTTTCAAGAAGTGA

Protein sequence

MVVSSTIPICHGRMESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISFYKDIGSNIDIVSALLVCIAPSWVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIAMAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLILFIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQLAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNSYYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTESVNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSLYIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDEEVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKKFREDMDSLKEEVELYPHDVRQPEVESVHVHKEVTIFKK
Homology
BLAST of CmaCh13G002050 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 5.2e-25
Identity = 130/533 (24.39%), Postives = 233/533 (43.71%), Query Frame = 0

Query: 30  RWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISFYKDIGSNIDIVSALLV 89
           +W  L A+  +  S G  + +S +S H+K  L   Q  LN ++   D+G      S + +
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 90  CIAPSWVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIA--MAGNSQILANTVVL 149
              P  V+L   +A+ FVGY   W  +   +   T+ Y  V++   +AG S    NT   
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNII---TLPYSLVFLCCLLAGLSICWFNTACF 162

Query: 150 VICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLILFIAWCPSLITLLFA 209
           ++C+R+FP  R + L L   F G+ AA+ +  + AI    +   +L  +  P +++    
Sbjct: 163 ILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAAL 222

Query: 210 FSIREVKIV-------KHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQLAYTFVV 269
           + +     +          H+  VF  IL +  V+  F   L ++       +L +   V
Sbjct: 223 YPVLTKPSLDTTPDYDSRRHDSHVF-TILNVLAVITSF--HLLLSSSSTSSARLNFIGAV 282

Query: 270 VAIIGLLILPLFIAIRE---EMVQLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNSYYSN 329
           V ++  L  PL +  R+    ++   LN+ +      +  ++     S +   G  +   
Sbjct: 283 VLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGT 342

Query: 330 I--FNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTESVN 389
               N    GD+++    I  ++  L  +A   G    L   +NLGQI +S G   ++  
Sbjct: 343 AKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG---QNST 402

Query: 390 AMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPF--HNSL 449
            +V+I S F+F GR+ S    + + ++++  R     + LL + I F L+A       +L
Sbjct: 403 TLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTAL 462

Query: 450 YIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDE 509
             A+ LIG + G      +++ S+ FG     + +N+     PIG+ +L   +A  +Y+ 
Sbjct: 463 QTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGS-LLYGYIAASIYEA 522

Query: 510 EVK---TWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQ 544
                 T   S   +C G  CY  +F F   +S+LG   SL L  RT+  Y +
Sbjct: 523 NASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHR 564

BLAST of CmaCh13G002050 vs. ExPASy TrEMBL
Match: A0A6J1I3Q6 (uncharacterized protein LOC111469393 OS=Cucurbita maxima OX=3661 GN=LOC111469393 PE=4 SV=1)

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query: 14  MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISF 73
           MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISF
Sbjct: 1   MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISF 60

Query: 74  YKDIGSNIDIVSALLVCIAPSWVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIA 133
           YKDIGSNIDIVSALLVCIAPSWVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIA
Sbjct: 61  YKDIGSNIDIVSALLVCIAPSWVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIA 120

Query: 134 MAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL 193
           MAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL
Sbjct: 121 MAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL 180

Query: 194 FIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ 253
           FIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ
Sbjct: 181 FIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ 240

Query: 254 LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNS 313
           LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNS
Sbjct: 241 LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNS 300

Query: 314 YYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTES 373
           YYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTES
Sbjct: 301 YYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTES 360

Query: 374 VNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL 433
           VNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL
Sbjct: 361 VNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL 420

Query: 434 YIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDE 493
           YIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDE
Sbjct: 421 YIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDE 480

Query: 494 EVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKKFREDM 553
           EVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKKFREDM
Sbjct: 481 EVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKKFREDM 540

Query: 554 DSLKEEVELYPHDVRQPEVESVHVHKEVTIFKK 587
           DSLKEEVELYPHDVRQPEVESVHVHKEVTIFKK
Sbjct: 541 DSLKEEVELYPHDVRQPEVESVHVHKEVTIFKK 573

BLAST of CmaCh13G002050 vs. ExPASy TrEMBL
Match: A0A6J1EYR7 (uncharacterized protein LOC111440576 OS=Cucurbita moschata OX=3662 GN=LOC111440576 PE=4 SV=1)

HSP 1 Score: 1036.9 bits (2680), Expect = 3.1e-299
Identity = 537/573 (93.72%), Postives = 556/573 (97.03%), Query Frame = 0

Query: 14  MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISF 73
           MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFY Y+YF EHIKETLQCDQTTLNKISF
Sbjct: 1   MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYLYAYFPEHIKETLQCDQTTLNKISF 60

Query: 74  YKDIGSNIDIVSALLVCIAPSWVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIA 133
           YKDIGSNID+VSALLV IAPSW+LLLICSALNF+GYFKIWQAVVGQ+ HPTVEYFGVYIA
Sbjct: 61  YKDIGSNIDVVSALLVYIAPSWILLLICSALNFIGYFKIWQAVVGQIAHPTVEYFGVYIA 120

Query: 134 MAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL 193
           +AGNSQILANTVVLVICVRNFPERRG+ILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL
Sbjct: 121 IAGNSQILANTVVLVICVRNFPERRGMILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL 180

Query: 194 FIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ 253
           FIAWCPSLITL FAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ
Sbjct: 181 FIAWCPSLITLFFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ 240

Query: 254 LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNS 313
           LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNM KFEKS+SKPQ PIHAISHNQNHGNS
Sbjct: 241 LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMIKFEKSQSKPQRPIHAISHNQNHGNS 300

Query: 314 YYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTES 373
           +YSNIFNKPERGDDYTILQG FSIDMLLICL +SIG GSSLTAMDNLGQIGESQGYSTES
Sbjct: 301 HYSNIFNKPERGDDYTILQGFFSIDMLLICLTISIGFGSSLTAMDNLGQIGESQGYSTES 360

Query: 374 VNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL 433
           VNAMVSIISIFNF+GRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL
Sbjct: 361 VNAMVSIISIFNFVGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL 420

Query: 434 YIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDE 493
           YIASILIGF+FGSQVLLDITMISE FGLEHYAMFYN+AQL+CPIGTYVLKVLVAGKLYDE
Sbjct: 421 YIASILIGFSFGSQVLLDITMISEIFGLEHYAMFYNIAQLSCPIGTYVLKVLVAGKLYDE 480

Query: 494 EVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKKFREDM 553
            VKTWSD  EYICHGEQCYR+SFTFLT +SLLGAAISLILVKRTREFYR+DIYKKFREDM
Sbjct: 481 AVKTWSDPTEYICHGEQCYRSSFTFLTGISLLGAAISLILVKRTREFYREDIYKKFREDM 540

Query: 554 DSLKEEVELYPHDVRQPEVESVHVHKEVTIFKK 587
           DSLKEEVELYPHDVRQPEVES+HVH+E TI KK
Sbjct: 541 DSLKEEVELYPHDVRQPEVESIHVHQEGTIIKK 573

BLAST of CmaCh13G002050 vs. ExPASy TrEMBL
Match: A0A6J1HLV5 (uncharacterized protein LOC111464764 OS=Cucurbita moschata OX=3662 GN=LOC111464764 PE=4 SV=1)

HSP 1 Score: 912.1 bits (2356), Expect = 1.2e-261
Identity = 478/585 (81.71%), Postives = 529/585 (90.43%), Query Frame = 0

Query: 2   VVSSTIPICHGRMESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKETL 61
           +V STIPI HGR+ + +FS+F RQV+ GRWFSLFASFLVMTSAGAFY YS+FS+ IKETL
Sbjct: 1   MVVSTIPIYHGRIGTVEFSSFTRQVLGGRWFSLFASFLVMTSAGAFYLYSHFSKDIKETL 60

Query: 62  QCDQTTLNKISFYKDIGSNIDIVSALLVCIAPSWVLLLICSALNFVGYFKIWQAVVGQVV 121
           QCDQTTLNKI FYKDIGSNIDI S LLV IAPSWVLL+ICSA+NF+GYFKIWQ VVG++V
Sbjct: 61  QCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVICSAVNFIGYFKIWQGVVGKIV 120

Query: 122 HPTVEYFGVYIAMAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYL 181
            P+VEYF  YIAM GNSQILAN+VVLV CVRNFPERRGVILGLLKGFLGLGAAVL+Q+YL
Sbjct: 121 QPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGVILGLLKGFLGLGAAVLSQIYL 180

Query: 182 AIYGHDTKSLILFIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTV 241
           AIYGHDTKSLILFIAW PSLITL+FAFSI+EVKIVKHPHEFRVFIQ LCITL+LA+F+ V
Sbjct: 181 AIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPHEFRVFIQFLCITLILAMFIIV 240

Query: 242 LTIAEQMVQFDQLAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPI 301
           LTI +++VQFDQ A+  V++AI+GLLILPLFIAIREEMV+ NLN MTKFEK ++   I  
Sbjct: 241 LTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMVRWNLNKMTKFEKCQTSASIIS 300

Query: 302 HAISHNQNHGNSYYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLG 361
           ++ S  Q+  NS  SNIFNKPERGDDYTILQG FSIDMLLICL MSIG GSSLTAMDNLG
Sbjct: 301 YSSSTLQHSRNSDLSNIFNKPERGDDYTILQGFFSIDMLLICLTMSIGIGSSLTAMDNLG 360

Query: 362 QIGESQGYSTESVNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIG 421
           QIGESQGYS ES+NA+V+IISIFNFLGRIFSGFVSEILLE+YKFPRPLMLTLTLLVSCIG
Sbjct: 361 QIGESQGYSAESINAIVTIISIFNFLGRIFSGFVSEILLEQYKFPRPLMLTLTLLVSCIG 420

Query: 422 FLLVAFPFHNSLYIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYV 481
           +LLVAFPF+NSLYIASILIGFAFGSQVLLDIT+ISE FGL+HYA+FYN+AQL+CPIGTYV
Sbjct: 421 YLLVAFPFNNSLYIASILIGFAFGSQVLLDITVISEIFGLKHYAIFYNIAQLSCPIGTYV 480

Query: 482 LKVLVAGKLYDEEVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFY 541
           L VLVAGKLYD+EVKTW DS EY CHGEQCY++SFT LT MSLLGAAISLILVKRTREFY
Sbjct: 481 LNVLVAGKLYDKEVKTWIDSSEYKCHGEQCYKDSFTILTGMSLLGAAISLILVKRTREFY 540

Query: 542 RQDIYKKFREDMDSLKEEVELYPHDVRQPEVESVHVHKEVTIFKK 587
           R DIY+KFREDMDSL+EEVELY  DVRQ E ESVHVHKEVTIFKK
Sbjct: 541 RGDIYRKFREDMDSLREEVELYSLDVRQNEDESVHVHKEVTIFKK 585

BLAST of CmaCh13G002050 vs. ExPASy TrEMBL
Match: A0A6J1I0L4 (uncharacterized protein LOC111469419 OS=Cucurbita maxima OX=3661 GN=LOC111469419 PE=4 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 3.6e-247
Identity = 453/453 (100.00%), Postives = 453/453 (100.00%), Query Frame = 0

Query: 134 MAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL 193
           MAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL
Sbjct: 1   MAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL 60

Query: 194 FIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ 253
           FIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ
Sbjct: 61  FIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ 120

Query: 254 LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNS 313
           LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNS
Sbjct: 121 LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNS 180

Query: 314 YYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTES 373
           YYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTES
Sbjct: 181 YYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTES 240

Query: 374 VNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL 433
           VNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL
Sbjct: 241 VNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL 300

Query: 434 YIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDE 493
           YIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDE
Sbjct: 301 YIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDE 360

Query: 494 EVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKKFREDM 553
           EVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKKFREDM
Sbjct: 361 EVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKKFREDM 420

Query: 554 DSLKEEVELYPHDVRQPEVESVHVHKEVTIFKK 587
           DSLKEEVELYPHDVRQPEVESVHVHKEVTIFKK
Sbjct: 421 DSLKEEVELYPHDVRQPEVESVHVHKEVTIFKK 453

BLAST of CmaCh13G002050 vs. ExPASy TrEMBL
Match: A0A5D3BP48 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2042G00080 PE=4 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 2.9e-188
Identity = 349/587 (59.45%), Postives = 444/587 (75.64%), Query Frame = 0

Query: 11  HGRMESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNK 70
           +G + S + ++F  QVVVGRWFSLFASFLVMT AG  Y ++Y+S  IK TLQCDQTTLN+
Sbjct: 35  NGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTLQCDQTTLNR 94

Query: 71  ISFYKDIGSNIDIVSALLVCIAPSWVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYFGV 130
           I FYKD+GSN+ +++ LL  +AP+W++LLI +A NF+GYFKIWQAV G++V PTV +F  
Sbjct: 95  IGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIVRPTVAFFCF 154

Query: 131 YIAMAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKS 190
           YI +  NSQ  ANT VLV CV+NFPERRGV+LGLLKGF+GL  A++TQ+Y+AIYGHDTKS
Sbjct: 155 YIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDTKS 214

Query: 191 LILFIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQ 250
           LIL IAW PS+++L+F ++IRE+K VKHP+EFRVF+Q LC+T++LAL LT L   ++ V 
Sbjct: 215 LILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTALIFVQKRVH 274

Query: 251 FDQLAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEK-------SRSKPQIPIHA 310
           FDQ A+  +V AI+ LL +PL IAIREE+V  NLN  T             S+P+ PIH+
Sbjct: 275 FDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIESSQPKPPIHS 334

Query: 311 ISHNQN----HGNSYYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDN 370
            S  Q+       S +SNIFNKPERG+DYT+LQ IFSIDML+IC  M IG G+SLTA+DN
Sbjct: 335 PSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGVGASLTAIDN 394

Query: 371 LGQIGESQGYSTESVNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSC 430
           LGQIGE+QGY +E++N  VS+ISI NF GRIFSGFVSEILLEK++FPRPLMLTL LL+S 
Sbjct: 395 LGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPLMLTLILLISS 454

Query: 431 IGFLLVAFPFHNSLYIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGT 490
           +G LLVAFPF +SLY+ASI+IGF+ GSQV L   MISE FGL+HY+  +N  QL+CPIG+
Sbjct: 455 LGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNFGQLSCPIGS 514

Query: 491 YVLKVLVAGKLYDEEVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTRE 550
           Y+L V+V GKLYDE  +  S+  +  C G  CY  SF  L  ++ + A ISLILV+RTRE
Sbjct: 515 YILNVMVTGKLYDEVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMISLILVQRTRE 574

Query: 551 FYRQDIYKKFREDMDSLKEEVELYPHDVRQPEVESVHVHKEVTIFKK 587
           FYR DIYKKF+EDM++LK EVE Y  D ++  + ++ V K    FKK
Sbjct: 575 FYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFKK 621

BLAST of CmaCh13G002050 vs. NCBI nr
Match: XP_022970393.1 (uncharacterized protein LOC111469393 [Cucurbita maxima])

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query: 14  MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISF 73
           MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISF
Sbjct: 1   MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISF 60

Query: 74  YKDIGSNIDIVSALLVCIAPSWVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIA 133
           YKDIGSNIDIVSALLVCIAPSWVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIA
Sbjct: 61  YKDIGSNIDIVSALLVCIAPSWVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIA 120

Query: 134 MAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL 193
           MAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL
Sbjct: 121 MAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL 180

Query: 194 FIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ 253
           FIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ
Sbjct: 181 FIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ 240

Query: 254 LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNS 313
           LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNS
Sbjct: 241 LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNS 300

Query: 314 YYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTES 373
           YYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTES
Sbjct: 301 YYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTES 360

Query: 374 VNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL 433
           VNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL
Sbjct: 361 VNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL 420

Query: 434 YIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDE 493
           YIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDE
Sbjct: 421 YIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDE 480

Query: 494 EVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKKFREDM 553
           EVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKKFREDM
Sbjct: 481 EVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKKFREDM 540

Query: 554 DSLKEEVELYPHDVRQPEVESVHVHKEVTIFKK 587
           DSLKEEVELYPHDVRQPEVESVHVHKEVTIFKK
Sbjct: 541 DSLKEEVELYPHDVRQPEVESVHVHKEVTIFKK 573

BLAST of CmaCh13G002050 vs. NCBI nr
Match: KAG6583517.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1059.7 bits (2739), Expect = 9.3e-306
Identity = 548/586 (93.52%), Postives = 566/586 (96.59%), Query Frame = 0

Query: 1   MVVSSTIPICHGRMESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKET 60
           MV SS IPICHG MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFY YSYF EHIKET
Sbjct: 1   MVASSAIPICHGTMESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYLYSYFPEHIKET 60

Query: 61  LQCDQTTLNKISFYKDIGSNIDIVSALLVCIAPSWVLLLICSALNFVGYFKIWQAVVGQV 120
           LQCDQTTLNKISFYKDIGSNID+VSALLV IAPSW+LLLICSALNF+GYFKIWQAVVGQ+
Sbjct: 61  LQCDQTTLNKISFYKDIGSNIDVVSALLVYIAPSWILLLICSALNFIGYFKIWQAVVGQI 120

Query: 121 VHPTVEYFGVYIAMAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVY 180
            HPTVEYFGVYIA+AGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVY
Sbjct: 121 AHPTVEYFGVYIAIAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVY 180

Query: 181 LAIYGHDTKSLILFIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLT 240
           LAIYGHDTKSLILFIAWCPSLITL FAFSIREVKIVKHPHEFRVFIQILCITLVLALFLT
Sbjct: 181 LAIYGHDTKSLILFIAWCPSLITLFFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLT 240

Query: 241 VLTIAEQMVQFDQLAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIP 300
           VLTIAEQMVQFDQLAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNM KFEKS+SKPQ P
Sbjct: 241 VLTIAEQMVQFDQLAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMIKFEKSQSKPQRP 300

Query: 301 IHAISHNQNHGNSYYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNL 360
           IHAISHNQNHGNS+YSNIFNKPERGDDYTILQG FSIDMLLICL +SIG GSSLTAMDNL
Sbjct: 301 IHAISHNQNHGNSHYSNIFNKPERGDDYTILQGFFSIDMLLICLTISIGFGSSLTAMDNL 360

Query: 361 GQIGESQGYSTESVNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCI 420
           GQIGESQGYSTESVNAMVS+ISIFNF+GRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCI
Sbjct: 361 GQIGESQGYSTESVNAMVSVISIFNFVGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCI 420

Query: 421 GFLLVAFPFHNSLYIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTY 480
           GFLLVAFPFHNSLYIASILIGF+FGSQVLLDITMISE FGLEHYAMFYN+AQL+CPIGTY
Sbjct: 421 GFLLVAFPFHNSLYIASILIGFSFGSQVLLDITMISEIFGLEHYAMFYNIAQLSCPIGTY 480

Query: 481 VLKVLVAGKLYDEEVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREF 540
           VLKVLVAGKLYDE VKTWSD  EYICHGEQCYR+SFTFLT +SLLGAAISLILVKRTREF
Sbjct: 481 VLKVLVAGKLYDEAVKTWSDPTEYICHGEQCYRSSFTFLTGISLLGAAISLILVKRTREF 540

Query: 541 YRQDIYKKFREDMDSLKEEVELYPHDVRQPEVESVHVHKEVTIFKK 587
           YR+DIYKKFREDMDSLKEEVELYPHDVRQPEVES+HVH+E TI KK
Sbjct: 541 YREDIYKKFREDMDSLKEEVELYPHDVRQPEVESIHVHQEGTIIKK 586

BLAST of CmaCh13G002050 vs. NCBI nr
Match: XP_022933331.1 (uncharacterized protein LOC111440576 [Cucurbita moschata] >XP_022964761.1 uncharacterized protein LOC111464762 [Cucurbita moschata] >XP_022964762.1 uncharacterized protein LOC111464763 [Cucurbita moschata])

HSP 1 Score: 1036.9 bits (2680), Expect = 6.4e-299
Identity = 537/573 (93.72%), Postives = 556/573 (97.03%), Query Frame = 0

Query: 14  MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISF 73
           MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFY Y+YF EHIKETLQCDQTTLNKISF
Sbjct: 1   MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYLYAYFPEHIKETLQCDQTTLNKISF 60

Query: 74  YKDIGSNIDIVSALLVCIAPSWVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIA 133
           YKDIGSNID+VSALLV IAPSW+LLLICSALNF+GYFKIWQAVVGQ+ HPTVEYFGVYIA
Sbjct: 61  YKDIGSNIDVVSALLVYIAPSWILLLICSALNFIGYFKIWQAVVGQIAHPTVEYFGVYIA 120

Query: 134 MAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL 193
           +AGNSQILANTVVLVICVRNFPERRG+ILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL
Sbjct: 121 IAGNSQILANTVVLVICVRNFPERRGMILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL 180

Query: 194 FIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ 253
           FIAWCPSLITL FAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ
Sbjct: 181 FIAWCPSLITLFFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ 240

Query: 254 LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNS 313
           LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNM KFEKS+SKPQ PIHAISHNQNHGNS
Sbjct: 241 LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMIKFEKSQSKPQRPIHAISHNQNHGNS 300

Query: 314 YYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTES 373
           +YSNIFNKPERGDDYTILQG FSIDMLLICL +SIG GSSLTAMDNLGQIGESQGYSTES
Sbjct: 301 HYSNIFNKPERGDDYTILQGFFSIDMLLICLTISIGFGSSLTAMDNLGQIGESQGYSTES 360

Query: 374 VNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL 433
           VNAMVSIISIFNF+GRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL
Sbjct: 361 VNAMVSIISIFNFVGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL 420

Query: 434 YIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDE 493
           YIASILIGF+FGSQVLLDITMISE FGLEHYAMFYN+AQL+CPIGTYVLKVLVAGKLYDE
Sbjct: 421 YIASILIGFSFGSQVLLDITMISEIFGLEHYAMFYNIAQLSCPIGTYVLKVLVAGKLYDE 480

Query: 494 EVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKKFREDM 553
            VKTWSD  EYICHGEQCYR+SFTFLT +SLLGAAISLILVKRTREFYR+DIYKKFREDM
Sbjct: 481 AVKTWSDPTEYICHGEQCYRSSFTFLTGISLLGAAISLILVKRTREFYREDIYKKFREDM 540

Query: 554 DSLKEEVELYPHDVRQPEVESVHVHKEVTIFKK 587
           DSLKEEVELYPHDVRQPEVES+HVH+E TI KK
Sbjct: 541 DSLKEEVELYPHDVRQPEVESIHVHQEGTIIKK 573

BLAST of CmaCh13G002050 vs. NCBI nr
Match: KAG6583519.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1034.6 bits (2674), Expect = 3.2e-298
Identity = 537/573 (93.72%), Postives = 555/573 (96.86%), Query Frame = 0

Query: 14  MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISF 73
           MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFY Y+YF EHIKETLQCDQTTLNKISF
Sbjct: 1   MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYLYAYFPEHIKETLQCDQTTLNKISF 60

Query: 74  YKDIGSNIDIVSALLVCIAPSWVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIA 133
           YKDIGSNID+VSALLV IAPSW+LLLICSALNF+GYFKIWQAVVGQ+ HPTVEYFGVYIA
Sbjct: 61  YKDIGSNIDVVSALLVYIAPSWILLLICSALNFIGYFKIWQAVVGQIAHPTVEYFGVYIA 120

Query: 134 MAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL 193
           +AGNSQILANTVVLVICVRNFPERRG+ILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL
Sbjct: 121 IAGNSQILANTVVLVICVRNFPERRGMILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL 180

Query: 194 FIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ 253
           FIAWCPSLITL FAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ
Sbjct: 181 FIAWCPSLITLFFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ 240

Query: 254 LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNS 313
           LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNM KFEKS+SKPQ PIHAISHNQNHGNS
Sbjct: 241 LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMIKFEKSQSKPQRPIHAISHNQNHGNS 300

Query: 314 YYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTES 373
           +YSNIFNKPERGDDYTILQG FSIDMLLICL +SIG GSSLTAMDNLGQIGESQGYSTES
Sbjct: 301 HYSNIFNKPERGDDYTILQGFFSIDMLLICLTISIGFGSSLTAMDNLGQIGESQGYSTES 360

Query: 374 VNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL 433
           VNAMVSIISIFNF+GRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL
Sbjct: 361 VNAMVSIISIFNFVGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL 420

Query: 434 YIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDE 493
           YIASILIGFAFGSQVLLDITMISE FGLEHYAMFYN+AQL+CPIGTYVLKVLVAGKLYDE
Sbjct: 421 YIASILIGFAFGSQVLLDITMISEIFGLEHYAMFYNIAQLSCPIGTYVLKVLVAGKLYDE 480

Query: 494 EVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKKFREDM 553
            VKTWSD  EYICHGEQCYR+SFTFLT +SLLGAAISLILVKRTREFYR+DIYKKFREDM
Sbjct: 481 AVKTWSDPTEYICHGEQCYRSSFTFLTGISLLGAAISLILVKRTREFYREDIYKKFREDM 540

Query: 554 DSLKEEVELYPHDVRQPEVESVHVHKEVTIFKK 587
           DSLKEEVELYPH VRQPEVES+HVH+E TI KK
Sbjct: 541 DSLKEEVELYPHYVRQPEVESIHVHQEGTIIKK 573

BLAST of CmaCh13G002050 vs. NCBI nr
Match: XP_023520962.1 (uncharacterized protein LOC111784523 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1033.9 bits (2672), Expect = 5.4e-298
Identity = 535/573 (93.37%), Postives = 557/573 (97.21%), Query Frame = 0

Query: 14  MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISF 73
           MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFY Y+YF EHIKETLQCDQTTLNKISF
Sbjct: 1   MESAQFSNFARQVVVGRWFSLFASFLVMTSAGAFYLYAYFPEHIKETLQCDQTTLNKISF 60

Query: 74  YKDIGSNIDIVSALLVCIAPSWVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIA 133
           Y+DIGSN DIVSALLV IAPSW+LLLI SALNF+GYFKIWQAVVGQ+VHPTVEYFGVYIA
Sbjct: 61  YRDIGSNTDIVSALLVYIAPSWILLLISSALNFIGYFKIWQAVVGQIVHPTVEYFGVYIA 120

Query: 134 MAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL 193
           +AGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL
Sbjct: 121 IAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLIL 180

Query: 194 FIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ 253
           FIAWCPSLITL FAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ
Sbjct: 181 FIAWCPSLITLFFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQ 240

Query: 254 LAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNS 313
           LAYTFVVVAIIGLL+LPLFIAIREEMVQLNLNNMTKFEKS+SKPQ PIHAISHNQNHGNS
Sbjct: 241 LAYTFVVVAIIGLLVLPLFIAIREEMVQLNLNNMTKFEKSQSKPQRPIHAISHNQNHGNS 300

Query: 314 YYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTES 373
           +YSNIFNKPERGDDYTILQG FSIDMLLICL +SIG GSSLTAMDNLGQIGESQGYST+S
Sbjct: 301 HYSNIFNKPERGDDYTILQGFFSIDMLLICLTISIGFGSSLTAMDNLGQIGESQGYSTQS 360

Query: 374 VNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL 433
           VNAMVSIISIFNF+GRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL
Sbjct: 361 VNAMVSIISIFNFVGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSL 420

Query: 434 YIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDE 493
           YIASILIGF+FGSQVLLDITMISE FGLEHYA+FYN+AQL+CPIGTYVLKVLVAGKLYDE
Sbjct: 421 YIASILIGFSFGSQVLLDITMISEIFGLEHYAVFYNIAQLSCPIGTYVLKVLVAGKLYDE 480

Query: 494 EVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKKFREDM 553
           E+KTW DS EYICHGEQCYR+SFTFLT +SLLGAAISLILVKRTREFYR+DIYKKFREDM
Sbjct: 481 EMKTWGDSTEYICHGEQCYRSSFTFLTGISLLGAAISLILVKRTREFYREDIYKKFREDM 540

Query: 554 DSLKEEVELYPHDVRQPEVESVHVHKEVTIFKK 587
           DSLKEEVELYPHDVRQPEVES+HVH+E TI KK
Sbjct: 541 DSLKEEVELYPHDVRQPEVESIHVHQEGTIIKK 573

BLAST of CmaCh13G002050 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 495.7 bits (1275), Expect = 5.0e-140
Identity = 272/554 (49.10%), Postives = 367/554 (66.25%), Query Frame = 0

Query: 29  GRWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISFYKDIGSNIDIVSALL 88
           GRWF +FASFL+M  AGA Y +  +S+ IK TL  DQTTLN + F+KD+G+N+ ++S L+
Sbjct: 16  GRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVGVLSGLI 75

Query: 89  VCIAPSWVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIAMAGNSQILANTVVLV 148
             + P+W +L I SA+NFVGYF IW  V G+V  P V    +YI +  NSQ  ANT  LV
Sbjct: 76  AEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFANTGALV 135

Query: 149 ICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLILFIAWCPSLITLLFAF 208
            CV+NFPE RGV+LGLLKG++GL  A+ TQ+Y AIYGHD+KSLIL IAW P+ ++L+F +
Sbjct: 136 TCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAAVSLVFVY 195

Query: 209 SIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQLAYTFVVVAIIGLLI 268
            IRE K+V+  +E  VF Q L I++ LALFL  + IAE+ V F + AY         LL 
Sbjct: 196 LIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASATICCALLF 255

Query: 269 LPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAISHNQNHG----------NSYYSNI 328
           +PL +++++E+   N+  +   E S  K + P   +  +Q+             S +S +
Sbjct: 256 VPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEKETKSCFSTV 315

Query: 329 FNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTESVNAMV 388
           F+ P RG+DYTILQ + S DM+++ +A   G GSSLTA+DNLGQIGES GY   +V++ V
Sbjct: 316 FSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPNHTVSSFV 375

Query: 389 SIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSLYIASI 448
           S++SI+N+ GR+FSGFVSE LL KYK PRPLM+TL LL+SC G LL+AFP   S+YIASI
Sbjct: 376 SLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPGSVYIASI 435

Query: 449 LIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDEEV--- 508
           L+GF+FG+Q+ L   +ISE FGL++Y+  +N  QLA P+G+Y+L V V G LYD+E    
Sbjct: 436 LMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLYDKEALKQ 495

Query: 509 -----KTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKKFR 565
                 T  D  +  C G QCY+  F  L  ++  GA +SL L  RTREFY+ DIYKKFR
Sbjct: 496 LTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKGDIYKKFR 555

BLAST of CmaCh13G002050 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 458.0 bits (1177), Expect = 1.1e-128
Identity = 256/561 (45.63%), Postives = 357/561 (63.64%), Query Frame = 0

Query: 25  QVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISFYKDIGSNIDIV 84
           Q++ GRWF  F S L+M++AGA Y +  +S  IKETL  DQTTLN +SF+KD+G+N+ ++
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 85  SALLVCIAPSWVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIAMAGNSQILANT 144
           + LL  + P W +LLI + LNF GYF IW AV  ++  P V +  +YI +  NSQ  ANT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 145 VVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLILFIAWCPSLITL 204
             LV CV+NFPE RGV+LG+LKG++GL  A++TQ+Y A YG DTK LIL I W P++++ 
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLPAIVSF 195

Query: 205 LFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQLAYTFVVVAII 264
            F  +IR +K+ +  +E +VF   L I+L LA FL V+ I  ++  F Q  +      +I
Sbjct: 196 AFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVVI 255

Query: 265 GLLILPLFIAIREEM-----VQLNLNNMTKFEKSRSKPQIPIHAISHNQNHGN------- 324
            LL+LP+ + I EE       Q+ LN+         KP++       +    +       
Sbjct: 256 VLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVEKV 315

Query: 325 ---SYYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGY 384
              S ++ +FN PERGDDYTILQ +FS+DML++ LA   G G +LTA+DNLGQIG S GY
Sbjct: 316 KTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLGY 375

Query: 385 STESVNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPF 444
              SV+  VS++SI+N+ GR+ SG VSEI L KYKFPRPLMLT+ LL+SC G LL+AF  
Sbjct: 376 PKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFNV 435

Query: 445 HNSLYIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGK 504
              LY+AS++IGF FG+Q  L   +ISE FGL++Y+  YN   +A PIG+Y+L V VAG 
Sbjct: 436 PGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAGY 495

Query: 505 LYDEEV--------KTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFY 563
           LYD E         KT  +  +  C G  C++ SF  +  ++L G  +S++LV RT++FY
Sbjct: 496 LYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKFY 555

BLAST of CmaCh13G002050 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 259.6 bits (662), Expect = 6.0e-69
Identity = 175/546 (32.05%), Postives = 287/546 (52.56%), Query Frame = 0

Query: 25  QVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISFYKDIGSNIDIV 84
           +++  +W ++ AS  +  ++GA Y +  +S  +K T   DQ+TL+ +S +KDIG+N  + 
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 85  SALLVCIAPS----------------WVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYF 144
           S LL   A S                WV+L + +   F GYF IW +V G +  P V   
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 145 GVYIAMAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDT 204
            +++ +A  SQ   NT  +V  V NF +  G  +G++KGFLGL  A+L Q+Y  +   D 
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDP 181

Query: 205 KSLILFIAWCPSLITLLFAFSIR--EVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAE 264
            S IL +A  P++++LL    +R  E  +            +  ++L++A +L ++ I +
Sbjct: 182 ASFILLLAVTPTVLSLLVMPLVRIYETSVADDKKHLN---GLSAVSLIIAAYLMIIIILK 241

Query: 265 QMVQFDQLAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQI--PIHAI 324
                   A    +V ++ +L LPL IA R +      + M K       P I  P    
Sbjct: 242 NTFGLSSWANIVTLVCLLVMLALPLLIARRAQR-----DGMEKTVPHDYSPLISSPKATT 301

Query: 325 SHNQNHGNSYYSNIFNKPERG--DDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQ 384
           S NQ+      S   +K E G  ++  +LQ +  +   L+ LAM  G GS L+ ++N+ Q
Sbjct: 302 SGNQS------SEGDSKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQ 361

Query: 385 IGESQGYSTESVNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGF 444
           IGES  YS+  +N++VS+ SI+NFLGR  +G+ S+ LL K  +PRPL++  TL    IG 
Sbjct: 362 IGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGH 421

Query: 445 LLVAFPFHNSLYIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVL 504
           L++A  F  +LY+ S+++G  +GSQ  L  T+ SE FG+ H    +N   +A PIG+Y+ 
Sbjct: 422 LIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIF 481

Query: 505 KVLVAGKLYDEEVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYR 549
            V + G +YD+      ++    C+G  C+R SF  +  ++  G  ++++L  RT+  YR
Sbjct: 482 SVRLIGYIYDKTASGEGNT----CYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLYR 529

BLAST of CmaCh13G002050 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 255.0 bits (650), Expect = 1.5e-67
Identity = 173/531 (32.58%), Postives = 284/531 (53.48%), Query Frame = 0

Query: 30  RWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISFYKDIGSNIDIVSALLV 89
           +W +  AS  + + +GA Y ++ +S  +K +   DQ+TL+ +S +KDIG    I+S  L 
Sbjct: 7   KWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISGFLY 66

Query: 90  CIAPS--------WVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIAMAGNSQIL 149
               S        WV++ +     FVG+F IW +VVG +  P V    +++ +AG+S   
Sbjct: 67  TAMTSKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHSLPF 126

Query: 150 ANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHD--TKSLILFIAWCP 209
            NT  +V   RNF +  G  +G+++GFLGL  A+L Q+Y A+ G +    + IL +A  P
Sbjct: 127 FNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLAIVP 186

Query: 210 SLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQLAYTFV 269
           +L+  L    +R  + V    + +    +  I++++A +L V+   E ++   +    F 
Sbjct: 187 TLVMFLAMPFVRVYETVTISDK-KHLDGLSAISMIIAAYLMVVITVENVLGLSRSMQIFS 246

Query: 270 VVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAISHNQNHGNSYYSNIF 329
            + ++ LL  PL +A+R       L    +   S   P +   A+    +      SNIF
Sbjct: 247 FILVLLLLASPLLVAVRA------LREKRQTLSSLDGPVLDTSALLDPPS------SNIF 306

Query: 330 NKPER--GDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTESVNAM 389
              +    +D  IL+ + +++  L+ LAM  G GS    ++N+ QIGES  YS+  +N++
Sbjct: 307 PDGDHLVAEDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESLRYSSVQLNSL 366

Query: 390 VSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNSLYIAS 449
           VS+ SI+NFLGR  +G+VS+  L K+ +PRP+ + +TL V  IG ++VA     SLY  S
Sbjct: 367 VSLWSIWNFLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASGVQGSLYAGS 426

Query: 450 ILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYDEEVKT 509
           +LIG A+GSQ  L  T+ SE FG+ H    Y    +A PIG+Y+L V V G  YD+    
Sbjct: 427 VLIGMAYGSQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIGYFYDKVASE 486

Query: 510 WSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKK 549
             +S    C G QC+R SF  +  ++L G+ ++ +L  RT +FY+  + K+
Sbjct: 487 DDNS----CFGSQCFRTSFMIMASVALFGSLVASVLFFRTHKFYKNLVAKR 520

BLAST of CmaCh13G002050 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 9.6e-67
Identity = 165/540 (30.56%), Postives = 287/540 (53.15%), Query Frame = 0

Query: 23  ARQVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISFYKDIGSNID 82
           A +++  +W ++ AS  +  SAG  Y +  +S  +K T   DQ+TL+ +S +KDIG N+ 
Sbjct: 2   ATEILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVG 61

Query: 83  IVSALLVCIAP--------------SWVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYF 142
           ++S L+   A                WV++LI + LNF GYF +W +V G +  P V   
Sbjct: 62  VLSGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVM 121

Query: 143 GVYIAMAGNSQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDT 202
            +++ +A  S    NT  +V  + NF +  G  +G++KGF+GL  A+L Q+Y  +   D 
Sbjct: 122 CLFMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDP 181

Query: 203 KSLILFIAWCPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQM 262
           K+ IL +A  PSL+++L    +R  K      E +    +  ++L++A +L +  I +  
Sbjct: 182 KTFILLLAIVPSLLSVLVMPLVRVYK-TSTVDEKKHLDGLSTLSLIIAAYLMITIILKST 241

Query: 263 VQFDQLAYTFVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAISHNQ 322
           +     A    +  ++ LL  PL +A+R         +    EK  S    P+       
Sbjct: 242 LSLPSWANAVTLAVLLVLLSSPLLVAVRA--------HRDSIEKPLSSVYSPL------V 301

Query: 323 NHGNSYYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQG 382
           ++  +  S      +      +LQ + ++D  L+ LAM  G GS ++ ++N+ QIGES  
Sbjct: 302 DNLEATTSGEILMLDEDKSLNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQIGESLR 361

Query: 383 YSTESVNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFP 442
           Y++  +N+++++ +I+NF+GR   G+VS+ LL +  +PRPL++  TL    IG L++A  
Sbjct: 362 YTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHLIIASG 421

Query: 443 FHNSLYIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAG 502
           F  +LY  SI++G  +GSQ  L  T+ SE FG++H    YN   +A P+G+Y+  V + G
Sbjct: 422 FQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFSVRLIG 481

Query: 503 KLYDEEVKTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKK 549
            +YD  +    ++    C+G  C+R ++  +  ++ LG  +S +LV RT+  YRQ I++K
Sbjct: 482 YIYDRTIIGEGNT----CYGPHCFRLAYVVIASVAFLGFLVSCVLVFRTKTIYRQ-IFEK 521

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I9E15.2e-2524.39Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A6J1I3Q60.0e+00100.00uncharacterized protein LOC111469393 OS=Cucurbita maxima OX=3661 GN=LOC111469393... [more]
A0A6J1EYR73.1e-29993.72uncharacterized protein LOC111440576 OS=Cucurbita moschata OX=3662 GN=LOC1114405... [more]
A0A6J1HLV51.2e-26181.71uncharacterized protein LOC111464764 OS=Cucurbita moschata OX=3662 GN=LOC1114647... [more]
A0A6J1I0L43.6e-247100.00uncharacterized protein LOC111469419 OS=Cucurbita maxima OX=3661 GN=LOC111469419... [more]
A0A5D3BP482.9e-18859.45Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
XP_022970393.10.0e+00100.00uncharacterized protein LOC111469393 [Cucurbita maxima][more]
KAG6583517.19.3e-30693.52Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022933331.16.4e-29993.72uncharacterized protein LOC111440576 [Cucurbita moschata] >XP_022964761.1 unchar... [more]
KAG6583519.13.2e-29893.72Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023520962.15.4e-29893.37uncharacterized protein LOC111784523 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G28120.15.0e-14049.10Major facilitator superfamily protein [more]
AT2G39210.11.1e-12845.63Major facilitator superfamily protein [more]
AT1G74780.16.0e-6932.05Nodulin-like / Major Facilitator Superfamily protein [more]
AT2G34355.11.5e-6732.58Major facilitator superfamily protein [more]
AT1G18940.19.6e-6730.56Nodulin-like / Major Facilitator Superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 328..543
e-value: 9.5E-13
score: 50.0
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 252..541
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 21..214
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 30..275
e-value: 1.1E-66
score: 224.8
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 15..558
NoneNo IPR availablePANTHERPTHR21576:SF29PROTEIN NUCLEAR FUSION DEFECTIVE 4coord: 15..558

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh13G002050.1CmaCh13G002050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity