CmaCh13G001460 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh13G001460 vs. ExPASy Swiss-Prot
Match: Q9LX73 (E3 UFM1-protein ligase 1 homolog OS=Arabidopsis thaliana OX=3702 GN=At3g46220 PE=1 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 8.8e-259
Identity = 490/835 (58.68%), Postives = 612/835 (73.29%), Query Frame = 0

Query: 450  MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 509
            MD ELLELQRQFEFAQQ KS++RLS+RNVVELVQKLQEL ++DF+LLHTVTGKEYIT E 
Sbjct: 1    MDDELLELQRQFEFAQQVKSSVRLSDRNVVELVQKLQELGVIDFDLLHTVTGKEYITQEQ 60

Query: 510  LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 569
            LR EI  EI KLGR+S+IDLAD IGVDLY++EKQA+ ++ +DP LML+QGEIISQSYWDS
Sbjct: 61   LRNEITREISKLGRVSVIDLADTIGVDLYHVEKQAQDVVLNDPGLMLVQGEIISQSYWDS 120

Query: 570  AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 629
              EEINERLQE SQIA+AE+A  LQVGSEL+ S+L  RLGTLVK RLEGGQLYTPAYV R
Sbjct: 121  IAEEINERLQECSQIAVAELAGQLQVGSELVQSVLEPRLGTLVKARLEGGQLYTPAYVER 180

Query: 630  VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 689
            V+AMVRGA+R   VP+NL+ +W+ LQQL+   +GASG+AV+ SFFQS+FN L+KE E+LG
Sbjct: 181  VTAMVRGASRGIFVPSNLSALWAPLQQLVQETNGASGVAVENSFFQSIFNRLLKEEEMLG 240

Query: 690  SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 749
            SLRAG HWTP  F+ AQKEC+DS FSQNS I+YE ++KLGI   + FLQSRYPDG PL+ 
Sbjct: 241  SLRAGTHWTPSAFATAQKECVDSSFSQNSYISYESMQKLGISQAVQFLQSRYPDGTPLAA 300

Query: 750  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 809
             FIH S+IEMLDS  ED +E+ SW +SL VLPSSF  QDA+K+LL CPSV+ ALK  KA 
Sbjct: 301  VFIHSSMIEMLDSATEDAIEQNSWIDSLSVLPSSFTSQDANKMLLLCPSVQSALKAEKAL 360

Query: 810  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 869
            I G+S++ S+ FIK +YD++EKE +  ++  S+       S S        S S ESI P
Sbjct: 361  ILGESYVLSSGFIKGIYDQIEKEADAFSIQASTATLIHPSSKS--------SESTESI-P 420

Query: 870  GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESS-TKSKKNQRKAKATASVQV 929
             N         +K SKKKKGKS  +T+    E   DD+E +  KSK+NQ+K + ++S Q 
Sbjct: 421  ANT--------DKGSKKKKGKS-ASTKAATVETVPDDEEDARPKSKRNQKKGRDSSSSQK 480

Query: 930  AETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLRPM 989
             ++KAGGKKESVK +ESN   P +EWV++K+   +P+ ++ G ++P +I++ L +H++PM
Sbjct: 481  LDSKAGGKKESVKAQESNNIIPPDEWVMKKIVDSVPEFEDDGTENPDSILKHLADHMKPM 540

Query: 990  LNNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLHR 1049
            L NS +ERRK  FTENA++M+RL+D  Q+KLDESF+++QLYEKALDLFEDDQS +VVLHR
Sbjct: 541  LINSLKERRKKIFTENADRMRRLIDDLQKKLDESFLNMQLYEKALDLFEDDQSTAVVLHR 600

Query: 1050 HLLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSNK 1109
            HLLRT+AA I D L H LD++NK+KNG EV E +  + V L + ERT +AK+  GSLS K
Sbjct: 601  HLLRTTAATIADTLLHGLDIHNKMKNGTEVEESKTQDLVLLDSSERTALAKNLNGSLSKK 660

Query: 1110 AVTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRKNTMVIVWVRFLS 1169
            A+ + EALEGKRV+TFM    DL EESG++ KKLDKKLERTLLHSYRK            
Sbjct: 661  ALALVEALEGKRVDTFMVTFRDLAEESGLVLKKLDKKLERTLLHSYRK------------ 720

Query: 1170 RAAKRKVEMPLLSVILKNSGKMLLLTEAGRSLLPARLDLTSQVSAEMDPIALLPKVVSLL 1229
                                                 DL SQVS E DPIALL KVVSLL
Sbjct: 721  -------------------------------------DLISQVSTESDPIALLAKVVSLL 768

Query: 1230 YVQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD 1284
            +++IH+KALQAPGRAI+ AIS LK+KLDE AYK L+DYQ+ATVTLL+L+SA+ G+
Sbjct: 781  FIKIHNKALQAPGRAIAAAISHLKEKLDESAYKTLTDYQTATVTLLALMSASSGE 768

BLAST of CmaCh13G001460 vs. ExPASy Swiss-Prot
Match: B8AXB6 (E3 UFM1-protein ligase 1 homolog OS=Oryza sativa subsp. indica OX=39946 GN=OsI_18219 PE=3 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 6.6e-230
Identity = 437/836 (52.27%), Postives = 585/836 (69.98%), Query Frame = 0

Query: 450  MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 509
            MDAELLELQRQ E AQ A+S++RLSERNVVELVQKLQE  I+DFELLHT +GKEYIT +H
Sbjct: 1    MDAELLELQRQLEAAQSARSSVRLSERNVVELVQKLQERGIIDFELLHTTSGKEYITSDH 60

Query: 510  LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 569
            L+ EI  EI+K GR SL+DL+D +GVDLY++E+Q++++++DDP LMLI GEI+SQSYWD+
Sbjct: 61   LKHEIKMEIKKRGRASLVDLSDILGVDLYHVERQSQKVVADDPSLMLINGEIMSQSYWDT 120

Query: 570  AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 629
              EEINE+LQE SQIALAEIAA L +GSEL+ ++L  RLGT+VKGRLEGGQLYTPAYV+R
Sbjct: 121  VTEEINEKLQERSQIALAEIAAQLHIGSELVVNILEPRLGTIVKGRLEGGQLYTPAYVSR 180

Query: 630  VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 689
            ++AMVRGA R  TVPTNL  +W+SLQ  L  + GASG++V+GSFFQS+FNGL+KE  VLG
Sbjct: 181  ITAMVRGAARGITVPTNLPSVWNSLQLQLQEMHGASGVSVEGSFFQSIFNGLLKEGVVLG 240

Query: 690  SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 749
            S+RAGV WTP +F+ AQKE +D+FFSQNS I YE L+KL IP P  +L++RYPDGI L  
Sbjct: 241  SVRAGVQWTPAVFAHAQKESVDAFFSQNSYIGYEVLQKLAIPQPKQYLEARYPDGIALEA 300

Query: 750  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 809
             F+HPS+++MLD+ V D +E G W ++L VLPS     DA+KIL  CPS++ A+K +KA 
Sbjct: 301  VFVHPSVVDMLDAAVGDTIENGQWIDALSVLPSYITGPDATKILSLCPSLQKAIKSSKAV 360

Query: 810  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 869
            +FG+S +FSN FIK ++DR+EKEM++  +  S+      +  S  ++G+D     +    
Sbjct: 361  VFGESCVFSNAFIKGIFDRLEKEMDSFGIKHSAGQGKPSNMSSEHRIGSDGKDLGD---- 420

Query: 870  GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQES-STKSKKNQRKAKATASVQV 929
             N++  IG   +K  KKK+GK +G+ +  A E   D++ES   K KK  RK K   S   
Sbjct: 421  -NDTSSIGASSDKGPKKKRGKVSGSAKGAAVEKDDDNEESIPVKGKKAHRKNKDAGS--S 480

Query: 930  AETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDE-HGIDDPATIVQPLVNHLRP 989
             + K GGKK S K KE N N   ++ + +K+ T+ P+L+E  G DD    ++ L +HLRP
Sbjct: 481  GDAKHGGKKASEKTKEDNTNIFPDDLIEQKVLTVAPELEELGGSDDLNGPLKLLSSHLRP 540

Query: 990  MLNNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLH 1049
            ML ++W ++R    +ENAE+ +RLLD+ Q++LDE+ +D+QLYEK+LD+FEDD + S +LH
Sbjct: 541  MLMDAWMKKRNTMLSENAERRRRLLDNLQKQLDEAVLDMQLYEKSLDVFEDDPATSAILH 600

Query: 1050 RHLLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSN 1109
            +HLLRT  AP+VD +   L   NKLKNG++V +  + E V LST +RT++AK  PGSLS 
Sbjct: 601  KHLLRTMGAPVVDKILLTLHKDNKLKNGMDVED--SEENVQLSTADRTSLAKDLPGSLSV 660

Query: 1110 KAVTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRKNTMVIVWVRFL 1169
            KA  +AE LEGKR ++FM AL D  EESG++ KKLDK+LER++LHSYRK           
Sbjct: 661  KAQALAETLEGKRFDSFMDALRDTAEESGLLFKKLDKRLERSMLHSYRK----------- 720

Query: 1170 SRAAKRKVEMPLLSVILKNSGKMLLLTEAGRSLLPARLDLTSQVSAEMDPIALLPKVVSL 1229
                                                  DLT+QVS+E DPI+ LPKVV+L
Sbjct: 721  --------------------------------------DLTAQVSSENDPISFLPKVVAL 778

Query: 1230 LYVQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD 1284
            L++Q ++KALQAPGRA+   I+ LKDK+  P YK+L+DY S TV +L+L +AA  D
Sbjct: 781  LFLQAYNKALQAPGRAVGAVIALLKDKIPAPTYKVLADYHSTTVKVLALQAAATED 778

BLAST of CmaCh13G001460 vs. ExPASy Swiss-Prot
Match: B9FM64 (E3 UFM1-protein ligase 1 homolog OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0117600 PE=3 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 6.6e-230
Identity = 437/836 (52.27%), Postives = 585/836 (69.98%), Query Frame = 0

Query: 450  MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 509
            MDAELLELQRQ E AQ A+S++RLSERNVVELVQKLQE  I+DFELLHT +GKEYIT +H
Sbjct: 1    MDAELLELQRQLEAAQSARSSVRLSERNVVELVQKLQERGIIDFELLHTTSGKEYITSDH 60

Query: 510  LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 569
            L+ EI  EI+K GR SL+DL+D +GVDLY++E+Q++++++DDP LMLI GEI+SQSYWD+
Sbjct: 61   LKHEIKMEIKKRGRASLVDLSDILGVDLYHVERQSQKVVADDPSLMLINGEIMSQSYWDT 120

Query: 570  AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 629
              EEINE+LQE SQIALAEIAA L +GSEL+ ++L  RLGT+VKGRLEGGQLYTPAYV+R
Sbjct: 121  VTEEINEKLQERSQIALAEIAAQLHIGSELVVNILEPRLGTIVKGRLEGGQLYTPAYVSR 180

Query: 630  VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 689
            ++AMVRGA R  TVPTNL  +W+SLQ  L  + GASG++V+GSFFQS+FNGL+KE  VLG
Sbjct: 181  ITAMVRGAARGITVPTNLPSVWNSLQLQLQEMHGASGVSVEGSFFQSIFNGLLKEGVVLG 240

Query: 690  SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 749
            S+RAGV WTP +F+ AQKE +D+FFSQNS I YE L+KL IP P  +L++RYPDGI L  
Sbjct: 241  SVRAGVQWTPAVFAHAQKESVDAFFSQNSYIGYEVLQKLAIPQPKQYLEARYPDGIALEA 300

Query: 750  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 809
             F+HPS+++MLD+ V D +E G W ++L VLPS     DA+KIL  CPS++ A+K +KA 
Sbjct: 301  VFVHPSVVDMLDAAVGDTIENGQWIDALSVLPSYITGPDATKILSLCPSLQKAIKSSKAV 360

Query: 810  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 869
            +FG+S +FSN FIK ++DR+EKEM++  +  S+      +  S  ++G+D     +    
Sbjct: 361  VFGESCVFSNAFIKGIFDRLEKEMDSFGIKHSAGQGKPSNMSSEHRIGSDGKDLGD---- 420

Query: 870  GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQES-STKSKKNQRKAKATASVQV 929
             N++  IG   +K  KKK+GK +G+ +  A E   D++ES   K KK  RK K   S   
Sbjct: 421  -NDTSSIGASSDKGPKKKRGKVSGSAKGAAVEKDDDNEESIPVKGKKAHRKNKDAGS--S 480

Query: 930  AETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDE-HGIDDPATIVQPLVNHLRP 989
             + K GGKK S K KE N N   ++ + +K+ T+ P+L+E  G DD    ++ L +HLRP
Sbjct: 481  GDAKHGGKKASEKTKEDNTNIFPDDLIEQKVLTVAPELEELGGSDDLNGPLKLLSSHLRP 540

Query: 990  MLNNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLH 1049
            ML ++W ++R    +ENAE+ +RLLD+ Q++LDE+ +D+QLYEK+LD+FEDD + S +LH
Sbjct: 541  MLMDAWMKKRNTMLSENAERRRRLLDNLQKQLDEAVLDMQLYEKSLDVFEDDPATSAILH 600

Query: 1050 RHLLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSN 1109
            +HLLRT  AP+VD +   L   NKLKNG++V +  + E V LST +RT++AK  PGSLS 
Sbjct: 601  KHLLRTMGAPVVDKILLTLHKDNKLKNGMDVED--SEENVQLSTADRTSLAKDLPGSLSV 660

Query: 1110 KAVTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRKNTMVIVWVRFL 1169
            KA  +AE LEGKR ++FM AL D  EESG++ KKLDK+LER++LHSYRK           
Sbjct: 661  KAQALAETLEGKRFDSFMDALRDTAEESGLLFKKLDKRLERSMLHSYRK----------- 720

Query: 1170 SRAAKRKVEMPLLSVILKNSGKMLLLTEAGRSLLPARLDLTSQVSAEMDPIALLPKVVSL 1229
                                                  DLT+QVS+E DPI+ LPKVV+L
Sbjct: 721  --------------------------------------DLTAQVSSENDPISFLPKVVAL 778

Query: 1230 LYVQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD 1284
            L++Q ++KALQAPGRA+   I+ LKDK+  P YK+L+DY S TV +L+L +AA  D
Sbjct: 781  LFLQAYNKALQAPGRAVGAVIALLKDKIPAPTYKVLADYHSTTVKVLALQAAATED 778

BLAST of CmaCh13G001460 vs. ExPASy Swiss-Prot
Match: Q940Z5 (Phenolic glucoside malonyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PMAT1 PE=1 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 3.5e-82
Identity = 177/465 (38.06%), Postives = 262/465 (56.34%), Query Frame = 0

Query: 6   SVKIIEICKVAPPPEASRAAAVPSSLPLTFFDVLWLRIYPIQRLFFYELPSNGISFIDEV 65
           S+K+I++ +V P    S  +    +LPLTFFD+LW +++ ++R+ FY+L      F D V
Sbjct: 9   SLKVIDVARVTPSNSDSSESL---TLPLTFFDLLWYKLHAVERVIFYKLTDASRPFFDSV 68

Query: 66  -VPKLKTSLSLTLRHYLPLAGNLVWLPQSEVPTIEFVDGDGVSLTVAESGADFYQLSGNG 125
            VP LKTSLS +L HYLPLAG LVW P    P I +   D VS TVAES ADF +L+G  
Sbjct: 69  IVPNLKTSLSSSLSHYLPLAGKLVWEPLDPKPKIVYTPNDAVSFTVAESNADFSRLTGKE 128

Query: 126 FREVSEFHPLVPQLPVSHDRAATIAIQVTIFQNKGFSIGITSHHAVVDGRTSTSFIKSWA 185
               +E +PLVP+L VS D A+ ++ QVT+F N+GF I + +HHAV+DG+T+T+F+KSWA
Sbjct: 129 PFPTTELYPLVPELHVSDDSASAVSFQVTLFPNQGFCISVNAHHAVLDGKTTTNFLKSWA 188

Query: 186 QICMNESSVPIANLMPFYDRSVIDDPKGLAKIYASAW-------MNQDGPDN-RSLNLK- 245
           + C N+ S    +L+P YDR+VI DP  L     +AW            P+N +SL L  
Sbjct: 189 RTCKNQDSFLPQDLIPVYDRTVIKDPMDLDTKILNAWHRVAKVFTGGKEPENPKSLKLLW 248

Query: 246 LPKTKPSLIRSTFEFTHQNLEKLKKWVLKKNDE----------QMSSFVVTTAYLCVCTA 305
            P+  P + R T   T ++++KL++ + K++            ++S+FV+  +Y   C  
Sbjct: 249 SPEIGPDVFRYTLNLTREDIQKLRERLKKESSSSSVSSSPKELRLSTFVIVYSYALTCLI 308

Query: 306 KLDGLRDGNLL-FVFSGDVRSRLKPPVPLNYFGNCVVGSF-LSFERTELLGENGIALACE 365
           K  G      + + F+ D RS + PPVP +YFGNCV   F +S      + E G   A  
Sbjct: 309 KARGGDPSRPVGYGFAVDCRSLMVPPVPSSYFGNCVSACFKMSLTAETFMSEEGFLAAAR 368

Query: 366 QISKATKNLGGGGALKGAENLGTIVSEMTNDYSKIQPIGLAGSPRFGVYSADFGWGKPMK 425
            +S + + L    ALK  E    I+   T      Q + +AGS RFGVY  DFGWG+P K
Sbjct: 369 MVSDSVEALDENVALKIPE----ILEGFTTLSPGTQVLSVAGSTRFGVYGLDFGWGRPEK 428

Query: 426 VEIVSAESPLVFSLSDSRNSDVGMEIAVVKERDQMETFAALFNEG 449
           V +VS +     S ++SR+   G+E+    ++ +M+    L ++G
Sbjct: 429 VVVVSIDQGEAISFAESRDGSGGVELGFSLKKHEMDVLVDLLHKG 466

BLAST of CmaCh13G001460 vs. ExPASy Swiss-Prot
Match: Q9LJB4 (Malonyl-CoA:anthocyanidin 5-O-glucoside-6''-O-malonyltransferase OS=Arabidopsis thaliana OX=3702 GN=5MAT PE=1 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 3.0e-81
Identity = 177/456 (38.82%), Postives = 267/456 (58.55%), Query Frame = 0

Query: 6   SVKIIEICKVAPPPEASRAAAVPSSLPLTFFDVLWLRIYPIQRLFFYELPSNGISFIDEV 65
           +V I+E+ +V+PP   S       +LPLT+FD+ WL+++P+ R+ FY +P    S    +
Sbjct: 7   AVNILEVVQVSPPSSNS------LTLPLTYFDLGWLKLHPVDRVLFYHVPELTRS---SL 66

Query: 66  VPKLKTSLSLTLRHYLPLAGNLVWLPQSEVPTIEFV--DGDGVSLTVAESGADFYQLSGN 125
           + KLK+SLS TL HYLPLAG LVW      P+I +   D D V LTVAES  D   LSG+
Sbjct: 67  ISKLKSSLSATLLHYLPLAGRLVWDSIKTKPSIVYSPDDKDAVYLTVAESNGDLSHLSGD 126

Query: 126 GFREVSEFHPLVPQLPVSHDRAATIAIQVTIFQNKGFSIGITSHHAVVDGRTSTSFIKSW 185
             R  +EFH LVP+LPVS + A  +A+QVT F N+GFS+G+T+HHAV+DG+T+  F+K+W
Sbjct: 127 EPRPATEFHSLVPELPVSDESARVLAVQVTFFPNQGFSLGVTAHHAVLDGKTTAMFLKAW 186

Query: 186 AQICMNESSVPIANLMPFYDRSVIDDPKGLAKIYASAWMNQDGPDNRSLNLKLPKTK--- 245
           A  C  E      +L+P  DR ++ DP GL     + W++     N   +LKL  +K   
Sbjct: 187 AHNCKQEQEALPHDLVPSLDRIIVQDPTGLETKLLNRWIS---ASNNKPSLKLFPSKIIG 246

Query: 246 PSLIRSTFEFTHQNLEKLKKWVLKKNDE---QMSSFVVTTAYLCVCTAKLDGLRDGNLLF 305
             ++R T+  T ++++KL++ V  ++     ++S+FV+T AY+  C  K+ G      + 
Sbjct: 247 SDILRVTYRLTREDIKKLRERVETESHAKQLRLSTFVITYAYVITCMVKMRGGDPTRFVC 306

Query: 306 V-FSGDVRSRLKPPVPLNYFGNCVVGSFLSFERTELLGENGIALACEQISKATKNLGGGG 365
           V F+ D RSRL PP+P  +FGNC+VGS     + E + E G     +    A + L G  
Sbjct: 307 VGFASDFRSRLNPPLPPTFFGNCIVGSGDFDVKAEPILEEGEG---KGFITAVETLTGWV 366

Query: 366 ALKGAENLGTIVSEMTNDYSKIQP----IGLAGSPRFGVYSADFGWGKPMKVEIVSAESP 425
                EN+   +      + +++P    I +AGS R G+Y +DFGWGKP+KVEIV+ +  
Sbjct: 367 NGLCPENIEKNMLLPFEAFKRMEPGRQMISVAGSTRLGIYGSDFGWGKPVKVEIVTIDKD 426

Query: 426 LVFSLSDSRNSDVGMEIAVVKERDQMETFAALFNEG 449
              SLS+S +   G+E+ V  ++D +E F +LF+ G
Sbjct: 427 ASVSLSESGDGSGGVEVGVCLKKDDVERFGSLFSIG 447

BLAST of CmaCh13G001460 vs. ExPASy TrEMBL
Match: A0A6J1I5Q5 (E3 UFM1-protein ligase 1 homolog OS=Cucurbita maxima OX=3661 GN=LOC111469466 PE=3 SV=1)

HSP 1 Score: 1461.4 bits (3782), Expect = 0.0e+00
Identity = 785/834 (94.12%), Postives = 785/834 (94.12%), Query Frame = 0

Query: 450  MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 509
            MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 1    MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 510  LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 569
            LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS
Sbjct: 61   LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120

Query: 570  AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 629
            AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR
Sbjct: 121  AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 630  VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 689
            VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG
Sbjct: 181  VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 240

Query: 690  SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 749
            SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST
Sbjct: 241  SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 300

Query: 750  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 809
            TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF
Sbjct: 301  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 360

Query: 810  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 869
            IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP
Sbjct: 361  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 420

Query: 870  GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKATASVQVA 929
            GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKATASVQVA
Sbjct: 421  GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKATASVQVA 480

Query: 930  ETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLRPML 989
            ETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLRPML
Sbjct: 481  ETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLRPML 540

Query: 990  NNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLHRH 1049
            NNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLHRH
Sbjct: 541  NNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLHRH 600

Query: 1050 LLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSNKA 1109
            LLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSNKA
Sbjct: 601  LLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSNKA 660

Query: 1110 VTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRKNTMVIVWVRFLSR 1169
            VTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRK             
Sbjct: 661  VTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRK------------- 720

Query: 1170 AAKRKVEMPLLSVILKNSGKMLLLTEAGRSLLPARLDLTSQVSAEMDPIALLPKVVSLLY 1229
                                                DLTSQVSAEMDPIALLPKVVSLLY
Sbjct: 721  ------------------------------------DLTSQVSAEMDPIALLPKVVSLLY 780

Query: 1230 VQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD 1284
            VQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD
Sbjct: 781  VQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD 785

BLAST of CmaCh13G001460 vs. ExPASy TrEMBL
Match: A0A0A0LV94 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G498310 PE=3 SV=1)

HSP 1 Score: 1324.7 bits (3427), Expect = 0.0e+00
Identity = 709/837 (84.71%), Postives = 748/837 (89.37%), Query Frame = 0

Query: 447  EGKMDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYIT 506
            EG MD ELLELQRQFEFAQQAKS+IRLSERNVVELVQKLQELRILDFELLHTVTGKEYIT
Sbjct: 28   EGNMDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYIT 87

Query: 507  PEHLRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSY 566
            PEHLRREILAEIEKLGRISLIDLAD IGVDLYYIEKQAEQI+SDDP+L LIQGEIISQSY
Sbjct: 88   PEHLRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPQLTLIQGEIISQSY 147

Query: 567  WDSAGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAY 626
            WDS  EEINERLQESSQIALAEIAA+LQVGSELLASML QRLGTLVKGRLEGGQLYTPAY
Sbjct: 148  WDSVAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAY 207

Query: 627  VARVSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENE 686
            VARVSAMVRGATRA TVPTNLTVIWS+LQQLL GIDGASGIAVD SFFQSLFNG++KENE
Sbjct: 208  VARVSAMVRGATRAITVPTNLTVIWSTLQQLLQGIDGASGIAVDASFFQSLFNGIMKENE 267

Query: 687  VLGSLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIP 746
            VLGSLRAGVHWTP IFSIAQKE IDSFFSQNS+I+Y+FLRKLGIPNPI +LQSRYPDGIP
Sbjct: 268  VLGSLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYDFLRKLGIPNPIQYLQSRYPDGIP 327

Query: 747  LSTTFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCN 806
            LSTTFIHPSIIEMLDST+EDILERGSW NSLLVLPSSFEPQDASKILLSCPSV+ ALK N
Sbjct: 328  LSTTFIHPSIIEMLDSTIEDILERGSWANSLLVLPSSFEPQDASKILLSCPSVQGALKSN 387

Query: 807  KAFIFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAES 866
            KA IFGDSFIFSN FIKDLYDRMEKEMET+ VPGSST  FSGDS S SK+GNDPSMS ES
Sbjct: 388  KALIFGDSFIFSNTFIKDLYDRMEKEMETITVPGSSTGIFSGDSQSSSKLGNDPSMSTES 447

Query: 867  IEPGNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKATASV 926
            IE GN+SGK G+IM+KKSKKKKGKS GNTQ+ AAEGALDDQESSTKSKKNQRK + T++V
Sbjct: 448  IETGNDSGKTGDIMDKKSKKKKGKSIGNTQSTAAEGALDDQESSTKSKKNQRKTRGTSNV 507

Query: 927  QVAETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLR 986
            QVAETKAGGKKES K KESNINYPTEEWVIEK+KTLIPDL+EHGIDDP  IVQPL NHLR
Sbjct: 508  QVAETKAGGKKESAKTKESNINYPTEEWVIEKIKTLIPDLEEHGIDDPTIIVQPLANHLR 567

Query: 987  PMLNNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVL 1046
            PMLNN W+ERRKA FTENAEKMKRLLD+TQQKLDESF++LQLYEKALDLFEDDQSISV+L
Sbjct: 568  PMLNNLWRERRKALFTENAEKMKRLLDNTQQKLDESFLNLQLYEKALDLFEDDQSISVIL 627

Query: 1047 HRHLLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLS 1106
            HRHLLRT+AAPIVDMLFHNLDLYNKLKNGIEVAE+QNSE V+LSTGERTTIAKSFPGSLS
Sbjct: 628  HRHLLRTTAAPIVDMLFHNLDLYNKLKNGIEVAELQNSEAVALSTGERTTIAKSFPGSLS 687

Query: 1107 NKAVTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRKNTMVIVWVRF 1166
            NKAVTVAEALEGKRVETF++ALGDLVEESGMI KKLDKKLERTLLHSYRK          
Sbjct: 688  NKAVTVAEALEGKRVETFINALGDLVEESGMIPKKLDKKLERTLLHSYRK---------- 747

Query: 1167 LSRAAKRKVEMPLLSVILKNSGKMLLLTEAGRSLLPARLDLTSQVSAEMDPIALLPKVVS 1226
                                                   +LTSQVSAEMDPIALLPKVVS
Sbjct: 748  ---------------------------------------ELTSQVSAEMDPIALLPKVVS 807

Query: 1227 LLYVQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD 1284
            LLYVQI+HKALQAPGRAISVAISRLKDKLD+ A+KILSDYQ+ATVTLLSLISAAVGD
Sbjct: 808  LLYVQIYHKALQAPGRAISVAISRLKDKLDDSAHKILSDYQTATVTLLSLISAAVGD 815

BLAST of CmaCh13G001460 vs. ExPASy TrEMBL
Match: A0A6J1GAG2 (E3 UFM1-protein ligase 1 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452413 PE=3 SV=1)

HSP 1 Score: 1317.0 bits (3407), Expect = 0.0e+00
Identity = 705/834 (84.53%), Postives = 745/834 (89.33%), Query Frame = 0

Query: 450  MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 509
            MD ELLELQRQFEFAQQAKS+IRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 1    MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 510  LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 569
            LRREILAEIEKLGRISLIDLAD IGVDLYY+EKQAEQIISDDPRLMLIQGEIISQSYWDS
Sbjct: 61   LRREILAEIEKLGRISLIDLADTIGVDLYYVEKQAEQIISDDPRLMLIQGEIISQSYWDS 120

Query: 570  AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 629
              EEINERLQESSQIALAEIAA+LQVGSELLASML QRLGTLVKGRLEGGQLYTPAYVAR
Sbjct: 121  VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 630  VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 689
            VSAMVRGATRA TVPTNLTVIWSSLQQLL GI+GASGIAVD SFFQSLFNGLVKENE+LG
Sbjct: 181  VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240

Query: 690  SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 749
            SLRAGVHWTP IFSIAQKEC+DSFFSQNSII+YEFLRKLG+PNPI FLQSRYPDGIPLST
Sbjct: 241  SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300

Query: 750  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 809
            TFIHPSI+EMLDSTVEDILERGSWTNSLLVLPSSFEPQDASK+LLSCPSVRV+LK N A 
Sbjct: 301  TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360

Query: 810  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 869
            IFGDSFIFSNIFIKDLY RMEKEMET+NVPGSS   FSGDSLS SK   DPSM +ESIE 
Sbjct: 361  IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSSKFQTDPSMFSESIET 420

Query: 870  GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKATASVQVA 929
            GNNSG+ GEIMEKKSKKKKGKSTGNTQ+ AAE ALDDQE STKSKKNQRKAKA++SVQVA
Sbjct: 421  GNNSGRTGEIMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480

Query: 930  ETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLRPML 989
            ETKAGGKKESVK KE++I YPTEEWVIEK+KTLIPDL+EHGIDDPA I+QPLVNHLRPML
Sbjct: 481  ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540

Query: 990  NNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLHRH 1049
            NNSW+ERRKA F+ENAEKMKRLLD+TQQKLD SF++LQLY KALDLFEDDQSISV+LHRH
Sbjct: 541  NNSWRERRKALFSENAEKMKRLLDNTQQKLDGSFLNLQLYGKALDLFEDDQSISVILHRH 600

Query: 1050 LLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSNKA 1109
            LLRT+A+PIVDMLFHNLDL+NKLKNGIEVAE+QNSEPVSLSTGERTTIAKSFPGSLSNKA
Sbjct: 601  LLRTTASPIVDMLFHNLDLHNKLKNGIEVAELQNSEPVSLSTGERTTIAKSFPGSLSNKA 660

Query: 1110 VTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRKNTMVIVWVRFLSR 1169
            VTVAEALEGK ++TF++AL DL EESGMI KKLDKKLERTLLHSYRK             
Sbjct: 661  VTVAEALEGKLMDTFINALEDLAEESGMIVKKLDKKLERTLLHSYRK------------- 720

Query: 1170 AAKRKVEMPLLSVILKNSGKMLLLTEAGRSLLPARLDLTSQVSAEMDPIALLPKVVSLLY 1229
                                                DLTSQVSAEMDPIALLPKVVSLLY
Sbjct: 721  ------------------------------------DLTSQVSAEMDPIALLPKVVSLLY 780

Query: 1230 VQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD 1284
            +QIHHKALQAPGRAIS AISRLKDKLD+ A+KILSDYQ+ATVTLLSLISAA GD
Sbjct: 781  IQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLISAAAGD 785

BLAST of CmaCh13G001460 vs. ExPASy TrEMBL
Match: A0A6J1GB94 (E3 UFM1-protein ligase 1 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452413 PE=3 SV=1)

HSP 1 Score: 1317.0 bits (3407), Expect = 0.0e+00
Identity = 705/834 (84.53%), Postives = 745/834 (89.33%), Query Frame = 0

Query: 450  MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 509
            MD ELLELQRQFEFAQQAKS+IRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 1    MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 510  LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 569
            LRREILAEIEKLGRISLIDLAD IGVDLYY+EKQAEQIISDDPRLMLIQGEIISQSYWDS
Sbjct: 61   LRREILAEIEKLGRISLIDLADTIGVDLYYVEKQAEQIISDDPRLMLIQGEIISQSYWDS 120

Query: 570  AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 629
              EEINERLQESSQIALAEIAA+LQVGSELLASML QRLGTLVKGRLEGGQLYTPAYVAR
Sbjct: 121  VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 630  VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 689
            VSAMVRGATRA TVPTNLTVIWSSLQQLL GI+GASGIAVD SFFQSLFNGLVKENE+LG
Sbjct: 181  VSAMVRGATRAITVPTNLTVIWSSLQQLLQGINGASGIAVDASFFQSLFNGLVKENEILG 240

Query: 690  SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 749
            SLRAGVHWTP IFSIAQKEC+DSFFSQNSII+YEFLRKLG+PNPI FLQSRYPDGIPLST
Sbjct: 241  SLRAGVHWTPNIFSIAQKECVDSFFSQNSIISYEFLRKLGVPNPIQFLQSRYPDGIPLST 300

Query: 750  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 809
            TFIHPSI+EMLDSTVEDILERGSWTNSLLVLPSSFEPQDASK+LLSCPSVRV+LK N A 
Sbjct: 301  TFIHPSIVEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKVLLSCPSVRVSLKSNDAL 360

Query: 810  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 869
            IFGDSFIFSNIFIKDLY RMEKEMET+NVPGSS   FSGDSLS SK   DPSM +ESIE 
Sbjct: 361  IFGDSFIFSNIFIKDLYARMEKEMETINVPGSSIGTFSGDSLSSSKFQTDPSMFSESIET 420

Query: 870  GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKATASVQVA 929
            GNNSG+ GEIMEKKSKKKKGKSTGNTQ+ AAE ALDDQE STKSKKNQRKAKA++SVQVA
Sbjct: 421  GNNSGRTGEIMEKKSKKKKGKSTGNTQSTAAESALDDQEPSTKSKKNQRKAKASSSVQVA 480

Query: 930  ETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLRPML 989
            ETKAGGKKESVK KE++I YPTEEWVIEK+KTLIPDL+EHGIDDPA I+QPLVNHLRPML
Sbjct: 481  ETKAGGKKESVKTKENDIYYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540

Query: 990  NNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLHRH 1049
            NNSW+ERRKA F+ENAEKMKRLLD+TQQKLD SF++LQLY KALDLFEDDQSISV+LHRH
Sbjct: 541  NNSWRERRKALFSENAEKMKRLLDNTQQKLDGSFLNLQLYGKALDLFEDDQSISVILHRH 600

Query: 1050 LLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSNKA 1109
            LLRT+A+PIVDMLFHNLDL+NKLKNGIEVAE+QNSEPVSLSTGERTTIAKSFPGSLSNKA
Sbjct: 601  LLRTTASPIVDMLFHNLDLHNKLKNGIEVAELQNSEPVSLSTGERTTIAKSFPGSLSNKA 660

Query: 1110 VTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRKNTMVIVWVRFLSR 1169
            VTVAEALEGK ++TF++AL DL EESGMI KKLDKKLERTLLHSYRK             
Sbjct: 661  VTVAEALEGKLMDTFINALEDLAEESGMIVKKLDKKLERTLLHSYRK------------- 720

Query: 1170 AAKRKVEMPLLSVILKNSGKMLLLTEAGRSLLPARLDLTSQVSAEMDPIALLPKVVSLLY 1229
                                                DLTSQVSAEMDPIALLPKVVSLLY
Sbjct: 721  ------------------------------------DLTSQVSAEMDPIALLPKVVSLLY 780

Query: 1230 VQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD 1284
            +QIHHKALQAPGRAIS AISRLKDKLD+ A+KILSDYQ+ATVTLLSLISAA GD
Sbjct: 781  IQIHHKALQAPGRAISFAISRLKDKLDDSAWKILSDYQTATVTLLSLISAAAGD 785

BLAST of CmaCh13G001460 vs. ExPASy TrEMBL
Match: A0A1S3C5N8 (E3 UFM1-protein ligase 1 homolog OS=Cucumis melo OX=3656 GN=LOC103497188 PE=3 SV=1)

HSP 1 Score: 1315.1 bits (3402), Expect = 0.0e+00
Identity = 705/834 (84.53%), Postives = 744/834 (89.21%), Query Frame = 0

Query: 450  MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 509
            MD ELLELQRQFEFAQQAKS+IRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 1    MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 510  LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 569
            LRREILAEIEKLGRISLIDLAD IGVDLYYIEKQAEQI+SDDP+L LIQGEIISQSYWDS
Sbjct: 61   LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPQLTLIQGEIISQSYWDS 120

Query: 570  AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 629
              EEINERLQESSQIALAEIAA+LQVGSELLASML QRLGTLVKGRLEGGQLYTPAYVAR
Sbjct: 121  VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 630  VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 689
            VSAMVRGATRA TVPTNLTVIWSSLQQLL GIDGASGIAVD SFFQSLFNG++KENEVLG
Sbjct: 181  VSAMVRGATRAITVPTNLTVIWSSLQQLLQGIDGASGIAVDASFFQSLFNGIMKENEVLG 240

Query: 690  SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 749
            SLRAGVHWTP IFSIAQKE IDSFFSQNS+I+YEFLRKLGIPNPI FLQSRYPDGIPLST
Sbjct: 241  SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYEFLRKLGIPNPIQFLQSRYPDGIPLST 300

Query: 750  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 809
            TFIHPSIIEMLDS +EDILERGSW NSLLVLPSSFEPQDASK+LLSCPSV+ ALK NKA 
Sbjct: 301  TFIHPSIIEMLDSAIEDILERGSWANSLLVLPSSFEPQDASKVLLSCPSVQGALKSNKAL 360

Query: 810  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 869
            IFGDSFIFSNIFIKDL+DRMEKEMET+ VPGSST  FSGDS S SK+G DPSMS ESIE 
Sbjct: 361  IFGDSFIFSNIFIKDLFDRMEKEMETIIVPGSSTGIFSGDSQSSSKLGIDPSMSTESIEM 420

Query: 870  GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKATASVQVA 929
            GN+SGK GEI+EKKSKKKKGKS GNTQ+  AEGALDDQESSTKSKKNQRK + T++VQVA
Sbjct: 421  GNDSGKTGEIVEKKSKKKKGKSVGNTQSTTAEGALDDQESSTKSKKNQRKTRGTSNVQVA 480

Query: 930  ETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLRPML 989
            ETK GGKKESVK KESNINYPTEEWVIEK+KTLIPDL+EHGIDDP  IVQPL NHLRPML
Sbjct: 481  ETKTGGKKESVKTKESNINYPTEEWVIEKIKTLIPDLEEHGIDDPTIIVQPLANHLRPML 540

Query: 990  NNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLHRH 1049
            NN W+ERRKA FTENAEKMK LLD+TQQKLDESF++LQLYEKALDLFEDDQSISV+LHRH
Sbjct: 541  NNLWRERRKALFTENAEKMKHLLDNTQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH 600

Query: 1050 LLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSNKA 1109
            LLRT+AAPIVDMLFHNLDLYNKLKNGIEVAE+QNSEPV+LSTGER TIAKSFPGSLSNKA
Sbjct: 601  LLRTTAAPIVDMLFHNLDLYNKLKNGIEVAELQNSEPVALSTGERITIAKSFPGSLSNKA 660

Query: 1110 VTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRKNTMVIVWVRFLSR 1169
            VTVAEALEGKRVETF++ALGDLVEESGMI+KKLDKKLERTLLHSYRK             
Sbjct: 661  VTVAEALEGKRVETFINALGDLVEESGMISKKLDKKLERTLLHSYRK------------- 720

Query: 1170 AAKRKVEMPLLSVILKNSGKMLLLTEAGRSLLPARLDLTSQVSAEMDPIALLPKVVSLLY 1229
                                                +LTSQVS+EMDPIALLPKVVSLLY
Sbjct: 721  ------------------------------------ELTSQVSSEMDPIALLPKVVSLLY 780

Query: 1230 VQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD 1284
            VQI+HKALQAPGRAISVAISRLKDKLD+ A+KILSDYQ+ATVTLLSLISAAVGD
Sbjct: 781  VQIYHKALQAPGRAISVAISRLKDKLDDSAHKILSDYQTATVTLLSLISAAVGD 785

BLAST of CmaCh13G001460 vs. NCBI nr
Match: XP_022970509.1 (E3 UFM1-protein ligase 1 homolog [Cucurbita maxima])

HSP 1 Score: 1461.4 bits (3782), Expect = 0.0e+00
Identity = 785/834 (94.12%), Postives = 785/834 (94.12%), Query Frame = 0

Query: 450  MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 509
            MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 1    MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 510  LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 569
            LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS
Sbjct: 61   LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120

Query: 570  AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 629
            AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR
Sbjct: 121  AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 630  VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 689
            VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG
Sbjct: 181  VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 240

Query: 690  SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 749
            SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST
Sbjct: 241  SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 300

Query: 750  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 809
            TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF
Sbjct: 301  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 360

Query: 810  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 869
            IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP
Sbjct: 361  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 420

Query: 870  GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKATASVQVA 929
            GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKATASVQVA
Sbjct: 421  GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKATASVQVA 480

Query: 930  ETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLRPML 989
            ETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLRPML
Sbjct: 481  ETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLRPML 540

Query: 990  NNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLHRH 1049
            NNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLHRH
Sbjct: 541  NNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLHRH 600

Query: 1050 LLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSNKA 1109
            LLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSNKA
Sbjct: 601  LLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSNKA 660

Query: 1110 VTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRKNTMVIVWVRFLSR 1169
            VTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRK             
Sbjct: 661  VTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRK------------- 720

Query: 1170 AAKRKVEMPLLSVILKNSGKMLLLTEAGRSLLPARLDLTSQVSAEMDPIALLPKVVSLLY 1229
                                                DLTSQVSAEMDPIALLPKVVSLLY
Sbjct: 721  ------------------------------------DLTSQVSAEMDPIALLPKVVSLLY 780

Query: 1230 VQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD 1284
            VQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD
Sbjct: 781  VQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD 785

BLAST of CmaCh13G001460 vs. NCBI nr
Match: XP_023519824.1 (E3 UFM1-protein ligase 1 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1429.1 bits (3698), Expect = 0.0e+00
Identity = 768/834 (92.09%), Postives = 776/834 (93.05%), Query Frame = 0

Query: 450  MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 509
            MDAELLELQRQFEFAQQAKS+IRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 1    MDAELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 510  LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 569
            LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS
Sbjct: 61   LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120

Query: 570  AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 629
            A EEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR
Sbjct: 121  AAEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 630  VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 689
            VSAMVRGATRA TVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG
Sbjct: 181  VSAMVRGATRAITVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 240

Query: 690  SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 749
            SLRAGVHWTPKIFSIAQKECIDSFFSQNS+INYEFLRKLGIPNPI FLQSRYPDGIPLST
Sbjct: 241  SLRAGVHWTPKIFSIAQKECIDSFFSQNSVINYEFLRKLGIPNPIQFLQSRYPDGIPLST 300

Query: 750  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 809
            TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSV+VALKCNKAF
Sbjct: 301  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKCNKAF 360

Query: 810  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 869
            IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTAN SGDSLSISKVGNDPSMSAESIEP
Sbjct: 361  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANISGDSLSISKVGNDPSMSAESIEP 420

Query: 870  GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKATASVQVA 929
            GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKAT SVQVA
Sbjct: 421  GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKATTSVQVA 480

Query: 930  ETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLRPML 989
            ETKAGGKKESVK KESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATI+QPLVNHLRPML
Sbjct: 481  ETKAGGKKESVKTKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATILQPLVNHLRPML 540

Query: 990  NNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLHRH 1049
            NNSW+ERRKA FTENAEKMKRLLDSTQQKLDESFI+LQLYEKALDLFEDDQSISVVLHRH
Sbjct: 541  NNSWRERRKALFTENAEKMKRLLDSTQQKLDESFINLQLYEKALDLFEDDQSISVVLHRH 600

Query: 1050 LLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSNKA 1109
            LLRTSAAPIVDMLFHNLDLYNKLKNGIE AEMQNSEPVSLSTGERTTIAKSFPGSLSNKA
Sbjct: 601  LLRTSAAPIVDMLFHNLDLYNKLKNGIEAAEMQNSEPVSLSTGERTTIAKSFPGSLSNKA 660

Query: 1110 VTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRKNTMVIVWVRFLSR 1169
            VTVAEALEGKRVETF+HALGDLVEESGMI+KKLDKKLERTLLHSYRK             
Sbjct: 661  VTVAEALEGKRVETFVHALGDLVEESGMISKKLDKKLERTLLHSYRK------------- 720

Query: 1170 AAKRKVEMPLLSVILKNSGKMLLLTEAGRSLLPARLDLTSQVSAEMDPIALLPKVVSLLY 1229
                                                DLTSQVSAEMDPIALLPKVVSLLY
Sbjct: 721  ------------------------------------DLTSQVSAEMDPIALLPKVVSLLY 780

Query: 1230 VQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD 1284
            VQIHHKALQAPGRAISVAISRLKDKLDE AYKILSDYQSATVTLLSLISAAVGD
Sbjct: 781  VQIHHKALQAPGRAISVAISRLKDKLDESAYKILSDYQSATVTLLSLISAAVGD 785

BLAST of CmaCh13G001460 vs. NCBI nr
Match: KAG6583453.1 (E3 UFM1-protein ligase 1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1422.9 bits (3682), Expect = 0.0e+00
Identity = 763/834 (91.49%), Postives = 775/834 (92.93%), Query Frame = 0

Query: 450  MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 509
            MDAELLELQRQFEFAQQAKS+IRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 1    MDAELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 510  LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 569
            LR EILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS
Sbjct: 61   LRHEILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 120

Query: 570  AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 629
            A EEINERLQESSQIALAEIAA+LQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYV+R
Sbjct: 121  AAEEINERLQESSQIALAEIAAELQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVSR 180

Query: 630  VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 689
            VSAMVRGATRA TVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG
Sbjct: 181  VSAMVRGATRAITVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 240

Query: 690  SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 749
            SLRAGVHWTPKIFSIAQKECIDSFFSQNS+INYEFLRKLGIPNPI FLQSRYPDGIPLST
Sbjct: 241  SLRAGVHWTPKIFSIAQKECIDSFFSQNSVINYEFLRKLGIPNPIQFLQSRYPDGIPLST 300

Query: 750  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 809
            TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSV+VALKCNKAF
Sbjct: 301  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKCNKAF 360

Query: 810  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 869
            IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP
Sbjct: 361  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 420

Query: 870  GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKATASVQVA 929
            GNNSGKIGEIMEKKSKKKKGKSTGNTQT+AAEG LDDQESSTKSKKNQRKAKAT SVQVA
Sbjct: 421  GNNSGKIGEIMEKKSKKKKGKSTGNTQTVAAEGVLDDQESSTKSKKNQRKAKATTSVQVA 480

Query: 930  ETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLRPML 989
            ETKAGGKKESVK KESNIN+PTEEWVIEKLKTLIPDLDEHGIDDPATI+QPLVNHLRPML
Sbjct: 481  ETKAGGKKESVKTKESNINHPTEEWVIEKLKTLIPDLDEHGIDDPATILQPLVNHLRPML 540

Query: 990  NNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLHRH 1049
            NNSW+ERRKA FTENAEKMKRLLDSTQQKLDESFI+LQLYEKALDLFEDDQSISVVLHRH
Sbjct: 541  NNSWRERRKALFTENAEKMKRLLDSTQQKLDESFINLQLYEKALDLFEDDQSISVVLHRH 600

Query: 1050 LLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSNKA 1109
            LLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLS GERTTIAKSFPGSLSNKA
Sbjct: 601  LLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSIGERTTIAKSFPGSLSNKA 660

Query: 1110 VTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRKNTMVIVWVRFLSR 1169
            VTVAEALEGKRVETFMHALGD+VEESGMI+KKLDKKLERTLLHSYRK             
Sbjct: 661  VTVAEALEGKRVETFMHALGDIVEESGMISKKLDKKLERTLLHSYRK------------- 720

Query: 1170 AAKRKVEMPLLSVILKNSGKMLLLTEAGRSLLPARLDLTSQVSAEMDPIALLPKVVSLLY 1229
                                                DLTSQVSAEMDPIALLPKVVSLLY
Sbjct: 721  ------------------------------------DLTSQVSAEMDPIALLPKVVSLLY 780

Query: 1230 VQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD 1284
            VQIHHKALQAPGRAISVAISRLKDKLDE AYKILSDYQSATVTLLSLISAAVGD
Sbjct: 781  VQIHHKALQAPGRAISVAISRLKDKLDESAYKILSDYQSATVTLLSLISAAVGD 785

BLAST of CmaCh13G001460 vs. NCBI nr
Match: XP_038894524.1 (E3 UFM1-protein ligase 1 homolog isoform X1 [Benincasa hispida])

HSP 1 Score: 1324.7 bits (3427), Expect = 0.0e+00
Identity = 712/834 (85.37%), Postives = 748/834 (89.69%), Query Frame = 0

Query: 450  MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 509
            MD ELLELQRQFEFAQQAKS+IRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 1    MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 510  LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 569
            LRREILAEIEKLGRISLIDLAD IGVDLYYIEKQAEQIISDDPRL LIQGEIISQSYWDS
Sbjct: 61   LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIISDDPRLTLIQGEIISQSYWDS 120

Query: 570  AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 629
              EEINERLQESSQIALAEIAA+LQVGSELLASML QRLGTLVKGRLEGGQLYTPAYVAR
Sbjct: 121  VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 630  VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 689
            VSAMVRGATRA TVPTNLTVIWSSLQQ+L GIDGASGIAVDGSFFQSLFN +VKENE+LG
Sbjct: 181  VSAMVRGATRAITVPTNLTVIWSSLQQILQGIDGASGIAVDGSFFQSLFNAIVKENEILG 240

Query: 690  SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 749
            SLRAGVHWTP IFSIAQKE IDSFFSQNS+I+Y+FLRKLGIPNPI FLQSRYPDGIPLST
Sbjct: 241  SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYDFLRKLGIPNPIQFLQSRYPDGIPLST 300

Query: 750  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 809
            TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSV+VALK NKA 
Sbjct: 301  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVQVALKSNKAL 360

Query: 810  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 869
            IFGDSFIFSNIFIKDLY RMEKEMET+NVPGSST   S DSLS SK GNDPSMSAESIE 
Sbjct: 361  IFGDSFIFSNIFIKDLYGRMEKEMETINVPGSSTGISSVDSLSSSKFGNDPSMSAESIET 420

Query: 870  GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKATASVQVA 929
            GN+ GK GEI+EKKSKKKKGKS GNTQ+IAA+GALDD E+S KSKKNQRKAKAT++VQVA
Sbjct: 421  GNDIGKTGEIVEKKSKKKKGKSFGNTQSIAADGALDDHETSMKSKKNQRKAKATSNVQVA 480

Query: 930  ETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLRPML 989
            ETKAGGKKESVK KE+NINYPTEEWVIEK+KTLIPDL+EHGIDDPA I+QPLVNHLRPML
Sbjct: 481  ETKAGGKKESVKAKENNINYPTEEWVIEKIKTLIPDLEEHGIDDPAIILQPLVNHLRPML 540

Query: 990  NNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLHRH 1049
            NNSW+ERRKA FTENAEKMKRLLD+TQQKLDE F++LQLYEKALDLFEDDQSISV+LHRH
Sbjct: 541  NNSWKERRKALFTENAEKMKRLLDNTQQKLDEPFLNLQLYEKALDLFEDDQSISVILHRH 600

Query: 1050 LLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSNKA 1109
            LLRT+AAPIVDMLFHNLDLY KLKNGIEVAE+QNSE VSL+TGERTTIAKSFPGSLSNKA
Sbjct: 601  LLRTTAAPIVDMLFHNLDLYKKLKNGIEVAELQNSESVSLNTGERTTIAKSFPGSLSNKA 660

Query: 1110 VTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRKNTMVIVWVRFLSR 1169
            VT AEALEGKRVETF+ ALGDLVEESG+I KKLDKKLERTLLHSYRK             
Sbjct: 661  VTAAEALEGKRVETFISALGDLVEESGLILKKLDKKLERTLLHSYRK------------- 720

Query: 1170 AAKRKVEMPLLSVILKNSGKMLLLTEAGRSLLPARLDLTSQVSAEMDPIALLPKVVSLLY 1229
                                                +LTSQVSAEMDPIALLPKVVSLLY
Sbjct: 721  ------------------------------------ELTSQVSAEMDPIALLPKVVSLLY 780

Query: 1230 VQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD 1284
            +QIHHKALQAPGRAISVAISRLKDKLD+ A+KILSDYQ+ATVTLLSLISAAVGD
Sbjct: 781  IQIHHKALQAPGRAISVAISRLKDKLDDSAFKILSDYQTATVTLLSLISAAVGD 785

BLAST of CmaCh13G001460 vs. NCBI nr
Match: XP_011658001.1 (E3 UFM1-protein ligase 1 homolog [Cucumis sativus] >KGN66067.2 hypothetical protein Csa_019872 [Cucumis sativus])

HSP 1 Score: 1320.4 bits (3416), Expect = 0.0e+00
Identity = 707/834 (84.77%), Postives = 746/834 (89.45%), Query Frame = 0

Query: 450  MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 509
            MD ELLELQRQFEFAQQAKS+IRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH
Sbjct: 1    MDDELLELQRQFEFAQQAKSSIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 60

Query: 510  LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 569
            LRREILAEIEKLGRISLIDLAD IGVDLYYIEKQAEQI+SDDP+L LIQGEIISQSYWDS
Sbjct: 61   LRREILAEIEKLGRISLIDLADTIGVDLYYIEKQAEQIVSDDPQLTLIQGEIISQSYWDS 120

Query: 570  AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 629
              EEINERLQESSQIALAEIAA+LQVGSELLASML QRLGTLVKGRLEGGQLYTPAYVAR
Sbjct: 121  VAEEINERLQESSQIALAEIAAELQVGSELLASMLDQRLGTLVKGRLEGGQLYTPAYVAR 180

Query: 630  VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 689
            VSAMVRGATRA TVPTNLTVIWS+LQQLL GIDGASGIAVD SFFQSLFNG++KENEVLG
Sbjct: 181  VSAMVRGATRAITVPTNLTVIWSTLQQLLQGIDGASGIAVDASFFQSLFNGIMKENEVLG 240

Query: 690  SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 749
            SLRAGVHWTP IFSIAQKE IDSFFSQNS+I+Y+FLRKLGIPNPI +LQSRYPDGIPLST
Sbjct: 241  SLRAGVHWTPNIFSIAQKESIDSFFSQNSVISYDFLRKLGIPNPIQYLQSRYPDGIPLST 300

Query: 750  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 809
            TFIHPSIIEMLDST+EDILERGSW NSLLVLPSSFEPQDASKILLSCPSV+ ALK NKA 
Sbjct: 301  TFIHPSIIEMLDSTIEDILERGSWANSLLVLPSSFEPQDASKILLSCPSVQGALKSNKAL 360

Query: 810  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 869
            IFGDSFIFSN FIKDLYDRMEKEMET+ VPGSST  FSGDS S SK+GNDPSMS ESIE 
Sbjct: 361  IFGDSFIFSNTFIKDLYDRMEKEMETITVPGSSTGIFSGDSQSSSKLGNDPSMSTESIET 420

Query: 870  GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESSTKSKKNQRKAKATASVQVA 929
            GN+SGK G+IM+KKSKKKKGKS GNTQ+ AAEGALDDQESSTKSKKNQRK + T++VQVA
Sbjct: 421  GNDSGKTGDIMDKKSKKKKGKSIGNTQSTAAEGALDDQESSTKSKKNQRKTRGTSNVQVA 480

Query: 930  ETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLRPML 989
            ETKAGGKKES K KESNINYPTEEWVIEK+KTLIPDL+EHGIDDP  IVQPL NHLRPML
Sbjct: 481  ETKAGGKKESAKTKESNINYPTEEWVIEKIKTLIPDLEEHGIDDPTIIVQPLANHLRPML 540

Query: 990  NNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLHRH 1049
            NN W+ERRKA FTENAEKMKRLLD+TQQKLDESF++LQLYEKALDLFEDDQSISV+LHRH
Sbjct: 541  NNLWRERRKALFTENAEKMKRLLDNTQQKLDESFLNLQLYEKALDLFEDDQSISVILHRH 600

Query: 1050 LLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSNKA 1109
            LLRT+AAPIVDMLFHNLDLYNKLKNGIEVAE+QNSE V+LSTGERTTIAKSFPGSLSNKA
Sbjct: 601  LLRTTAAPIVDMLFHNLDLYNKLKNGIEVAELQNSEAVALSTGERTTIAKSFPGSLSNKA 660

Query: 1110 VTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRKNTMVIVWVRFLSR 1169
            VTVAEALEGKRVETF++ALGDLVEESGMI KKLDKKLERTLLHSYRK             
Sbjct: 661  VTVAEALEGKRVETFINALGDLVEESGMIPKKLDKKLERTLLHSYRK------------- 720

Query: 1170 AAKRKVEMPLLSVILKNSGKMLLLTEAGRSLLPARLDLTSQVSAEMDPIALLPKVVSLLY 1229
                                                +LTSQVSAEMDPIALLPKVVSLLY
Sbjct: 721  ------------------------------------ELTSQVSAEMDPIALLPKVVSLLY 780

Query: 1230 VQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD 1284
            VQI+HKALQAPGRAISVAISRLKDKLD+ A+KILSDYQ+ATVTLLSLISAAVGD
Sbjct: 781  VQIYHKALQAPGRAISVAISRLKDKLDDSAHKILSDYQTATVTLLSLISAAVGD 785

BLAST of CmaCh13G001460 vs. TAIR 10
Match: AT3G46220.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2042 (InterPro:IPR018611); Has 559 Blast hits to 533 proteins in 154 species: Archae - 3; Bacteria - 32; Metazoa - 305; Fungi - 42; Plants - 50; Viruses - 3; Other Eukaryotes - 124 (source: NCBI BLink). )

HSP 1 Score: 895.2 bits (2312), Expect = 6.3e-260
Identity = 490/835 (58.68%), Postives = 612/835 (73.29%), Query Frame = 0

Query: 450  MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 509
            MD ELLELQRQFEFAQQ KS++RLS+RNVVELVQKLQEL ++DF+LLHTVTGKEYIT E 
Sbjct: 1    MDDELLELQRQFEFAQQVKSSVRLSDRNVVELVQKLQELGVIDFDLLHTVTGKEYITQEQ 60

Query: 510  LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 569
            LR EI  EI KLGR+S+IDLAD IGVDLY++EKQA+ ++ +DP LML+QGEIISQSYWDS
Sbjct: 61   LRNEITREISKLGRVSVIDLADTIGVDLYHVEKQAQDVVLNDPGLMLVQGEIISQSYWDS 120

Query: 570  AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 629
              EEINERLQE SQIA+AE+A  LQVGSEL+ S+L  RLGTLVK RLEGGQLYTPAYV R
Sbjct: 121  IAEEINERLQECSQIAVAELAGQLQVGSELVQSVLEPRLGTLVKARLEGGQLYTPAYVER 180

Query: 630  VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 689
            V+AMVRGA+R   VP+NL+ +W+ LQQL+   +GASG+AV+ SFFQS+FN L+KE E+LG
Sbjct: 181  VTAMVRGASRGIFVPSNLSALWAPLQQLVQETNGASGVAVENSFFQSIFNRLLKEEEMLG 240

Query: 690  SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 749
            SLRAG HWTP  F+ AQKEC+DS FSQNS I+YE ++KLGI   + FLQSRYPDG PL+ 
Sbjct: 241  SLRAGTHWTPSAFATAQKECVDSSFSQNSYISYESMQKLGISQAVQFLQSRYPDGTPLAA 300

Query: 750  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 809
             FIH S+IEMLDS  ED +E+ SW +SL VLPSSF  QDA+K+LL CPSV+ ALK  KA 
Sbjct: 301  VFIHSSMIEMLDSATEDAIEQNSWIDSLSVLPSSFTSQDANKMLLLCPSVQSALKAEKAL 360

Query: 810  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 869
            I G+S++ S+ FIK +YD++EKE +  ++  S+       S S        S S ESI P
Sbjct: 361  ILGESYVLSSGFIKGIYDQIEKEADAFSIQASTATLIHPSSKS--------SESTESI-P 420

Query: 870  GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESS-TKSKKNQRKAKATASVQV 929
             N         +K SKKKKGKS  +T+    E   DD+E +  KSK+NQ+K + ++S Q 
Sbjct: 421  ANT--------DKGSKKKKGKS-ASTKAATVETVPDDEEDARPKSKRNQKKGRDSSSSQK 480

Query: 930  AETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLRPM 989
             ++KAGGKKESVK +ESN   P +EWV++K+   +P+ ++ G ++P +I++ L +H++PM
Sbjct: 481  LDSKAGGKKESVKAQESNNIIPPDEWVMKKIVDSVPEFEDDGTENPDSILKHLADHMKPM 540

Query: 990  LNNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLHR 1049
            L NS +ERRK  FTENA++M+RL+D  Q+KLDESF+++QLYEKALDLFEDDQS +VVLHR
Sbjct: 541  LINSLKERRKKIFTENADRMRRLIDDLQKKLDESFLNMQLYEKALDLFEDDQSTAVVLHR 600

Query: 1050 HLLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSNK 1109
            HLLRT+AA I D L H LD++NK+KNG EV E +  + V L + ERT +AK+  GSLS K
Sbjct: 601  HLLRTTAATIADTLLHGLDIHNKMKNGTEVEESKTQDLVLLDSSERTALAKNLNGSLSKK 660

Query: 1110 AVTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRKNTMVIVWVRFLS 1169
            A+ + EALEGKRV+TFM    DL EESG++ KKLDKKLERTLLHSYRK            
Sbjct: 661  ALALVEALEGKRVDTFMVTFRDLAEESGLVLKKLDKKLERTLLHSYRK------------ 720

Query: 1170 RAAKRKVEMPLLSVILKNSGKMLLLTEAGRSLLPARLDLTSQVSAEMDPIALLPKVVSLL 1229
                                                 DL SQVS E DPIALL KVVSLL
Sbjct: 721  -------------------------------------DLISQVSTESDPIALLAKVVSLL 768

Query: 1230 YVQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD 1284
            +++IH+KALQAPGRAI+ AIS LK+KLDE AYK L+DYQ+ATVTLL+L+SA+ G+
Sbjct: 781  FIKIHNKALQAPGRAIAAAISHLKEKLDESAYKTLTDYQTATVTLLALMSASSGE 768

BLAST of CmaCh13G001460 vs. TAIR 10
Match: AT3G46220.2 (unknown protein; INVOLVED IN: biological_process unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2042 (InterPro:IPR018611). )

HSP 1 Score: 887.9 bits (2293), Expect = 1.0e-257
Identity = 490/843 (58.13%), Postives = 612/843 (72.60%), Query Frame = 0

Query: 450  MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 509
            MD ELLELQRQFEFAQQ KS++RLS+RNVVELVQKLQEL ++DF+LLHTVTGKEYIT E 
Sbjct: 1    MDDELLELQRQFEFAQQVKSSVRLSDRNVVELVQKLQELGVIDFDLLHTVTGKEYITQEQ 60

Query: 510  LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 569
            LR EI  EI KLGR+S+IDLAD IGVDLY++EKQA+ ++ +DP LML+QGEIISQSYWDS
Sbjct: 61   LRNEITREISKLGRVSVIDLADTIGVDLYHVEKQAQDVVLNDPGLMLVQGEIISQSYWDS 120

Query: 570  AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 629
              EEINERLQE SQIA+AE+A  LQVGSEL+ S+L  RLGTLVK RLEGGQLYTPAYV R
Sbjct: 121  IAEEINERLQECSQIAVAELAGQLQVGSELVQSVLEPRLGTLVKARLEGGQLYTPAYVER 180

Query: 630  VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 689
            V+AMVRGA+R   VP+NL+ +W+ LQQL+   +GASG+AV+ SFFQS+FN L+KE E+LG
Sbjct: 181  VTAMVRGASRGIFVPSNLSALWAPLQQLVQETNGASGVAVENSFFQSIFNRLLKEEEMLG 240

Query: 690  SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 749
            SLRAG HWTP  F+ AQKEC+DS FSQNS I+YE ++KLGI   + FLQSRYPDG PL+ 
Sbjct: 241  SLRAGTHWTPSAFATAQKECVDSSFSQNSYISYESMQKLGISQAVQFLQSRYPDGTPLAA 300

Query: 750  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 809
             FIH S+IEMLDS  ED +E+ SW +SL VLPSSF  QDA+K+LL CPSV+ ALK  KA 
Sbjct: 301  VFIHSSMIEMLDSATEDAIEQNSWIDSLSVLPSSFTSQDANKMLLLCPSVQSALKAEKAL 360

Query: 810  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 869
            I G+S++ S+ FIK +YD++EKE +  ++  S+       S S        S S ESI P
Sbjct: 361  ILGESYVLSSGFIKGIYDQIEKEADAFSIQASTATLIHPSSKS--------SESTESI-P 420

Query: 870  GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESS-TKSKKNQRKAKATASVQV 929
             N         +K SKKKKGKS  +T+    E   DD+E +  KSK+NQ+K + ++S Q 
Sbjct: 421  ANT--------DKGSKKKKGKS-ASTKAATVETVPDDEEDARPKSKRNQKKGRDSSSSQK 480

Query: 930  AETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHG--------IDDPATIVQP 989
             ++KAGGKKESVK +ESN   P +EWV++K+   +P+ ++ G         ++P +I++ 
Sbjct: 481  LDSKAGGKKESVKAQESNNIIPPDEWVMKKIVDSVPEFEDDGTSENFFLCTENPDSILKH 540

Query: 990  LVNHLRPMLNNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQ 1049
            L +H++PML NS +ERRK  FTENA++M+RL+D  Q+KLDESF+++QLYEKALDLFEDDQ
Sbjct: 541  LADHMKPMLINSLKERRKKIFTENADRMRRLIDDLQKKLDESFLNMQLYEKALDLFEDDQ 600

Query: 1050 SISVVLHRHLLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKS 1109
            S +VVLHRHLLRT+AA I D L H LD++NK+KNG EV E +  + V L + ERT +AK+
Sbjct: 601  STAVVLHRHLLRTTAATIADTLLHGLDIHNKMKNGTEVEESKTQDLVLLDSSERTALAKN 660

Query: 1110 FPGSLSNKAVTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRKNTMV 1169
              GSLS KA+ + EALEGKRV+TFM    DL EESG++ KKLDKKLERTLLHSYRK    
Sbjct: 661  LNGSLSKKALALVEALEGKRVDTFMVTFRDLAEESGLVLKKLDKKLERTLLHSYRK---- 720

Query: 1170 IVWVRFLSRAAKRKVEMPLLSVILKNSGKMLLLTEAGRSLLPARLDLTSQVSAEMDPIAL 1229
                                                         DL SQVS E DPIAL
Sbjct: 721  ---------------------------------------------DLISQVSTESDPIAL 776

Query: 1230 LPKVVSLLYVQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAA 1284
            L KVVSLL+++IH+KALQAPGRAI+ AIS LK+KLDE AYK L+DYQ+ATVTLL+L+SA+
Sbjct: 781  LAKVVSLLFIKIHNKALQAPGRAIAAAISHLKEKLDESAYKTLTDYQTATVTLLALMSAS 776

BLAST of CmaCh13G001460 vs. TAIR 10
Match: AT3G46220.3 (unknown protein; INVOLVED IN: biological_process unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF2042 (InterPro:IPR018611). )

HSP 1 Score: 861.7 bits (2225), Expect = 7.7e-250
Identity = 479/835 (57.37%), Postives = 599/835 (71.74%), Query Frame = 0

Query: 450  MDAELLELQRQFEFAQQAKSNIRLSERNVVELVQKLQELRILDFELLHTVTGKEYITPEH 509
            MD ELLELQRQFEFAQQ KS++RLS+RNVVELVQKLQEL ++DF+LLHTVTGKEYIT E 
Sbjct: 1    MDDELLELQRQFEFAQQVKSSVRLSDRNVVELVQKLQELGVIDFDLLHTVTGKEYITQEQ 60

Query: 510  LRREILAEIEKLGRISLIDLADNIGVDLYYIEKQAEQIISDDPRLMLIQGEIISQSYWDS 569
            LR EI  EI KLGR+S+IDLAD IGVDLY++EKQA+ ++ +DP LML+QGEIISQSYWDS
Sbjct: 61   LRNEITREISKLGRVSVIDLADTIGVDLYHVEKQAQDVVLNDPGLMLVQGEIISQSYWDS 120

Query: 570  AGEEINERLQESSQIALAEIAADLQVGSELLASMLVQRLGTLVKGRLEGGQLYTPAYVAR 629
              EEINERLQE SQIA+AE+A  LQVGSEL+ S+L  RLGTLVK RLEGGQLYTPAYV R
Sbjct: 121  IAEEINERLQECSQIAVAELAGQLQVGSELVQSVLEPRLGTLVKARLEGGQLYTPAYVER 180

Query: 630  VSAMVRGATRATTVPTNLTVIWSSLQQLLLGIDGASGIAVDGSFFQSLFNGLVKENEVLG 689
            V+AMVRGA+R   VP+NL+ +W+ LQQL+   +GASG+AV+ SFFQS+FN L+KE E+LG
Sbjct: 181  VTAMVRGASRGIFVPSNLSALWAPLQQLVQETNGASGVAVENSFFQSIFNRLLKEEEMLG 240

Query: 690  SLRAGVHWTPKIFSIAQKECIDSFFSQNSIINYEFLRKLGIPNPIHFLQSRYPDGIPLST 749
            SLRAG HWTP                 NS I+YE ++KLGI   + FLQSRYPDG PL+ 
Sbjct: 241  SLRAGTHWTP----------------SNSYISYESMQKLGISQAVQFLQSRYPDGTPLAA 300

Query: 750  TFIHPSIIEMLDSTVEDILERGSWTNSLLVLPSSFEPQDASKILLSCPSVRVALKCNKAF 809
             FIH S+IEMLDS  ED +E+ SW +SL VLPSSF  QDA+K+LL CPSV+ ALK  KA 
Sbjct: 301  VFIHSSMIEMLDSATEDAIEQNSWIDSLSVLPSSFTSQDANKMLLLCPSVQSALKAEKAL 360

Query: 810  IFGDSFIFSNIFIKDLYDRMEKEMETMNVPGSSTANFSGDSLSISKVGNDPSMSAESIEP 869
            I G+S++ S+ FIK +YD++EKE +  ++  S+       S S        S S ESI P
Sbjct: 361  ILGESYVLSSGFIKGIYDQIEKEADAFSIQASTATLIHPSSKS--------SESTESI-P 420

Query: 870  GNNSGKIGEIMEKKSKKKKGKSTGNTQTIAAEGALDDQESS-TKSKKNQRKAKATASVQV 929
             N         +K SKKKKGKS  +T+    E   DD+E +  KSK+NQ+K + ++S Q 
Sbjct: 421  ANT--------DKGSKKKKGKS-ASTKAATVETVPDDEEDARPKSKRNQKKGRDSSSSQK 480

Query: 930  AETKAGGKKESVKKKESNINYPTEEWVIEKLKTLIPDLDEHGIDDPATIVQPLVNHLRPM 989
             ++KAGGKKESVK +ESN   P +EWV++K+   +P+ ++ G ++P +I++ L +H++PM
Sbjct: 481  LDSKAGGKKESVKAQESNNIIPPDEWVMKKIVDSVPEFEDDGTENPDSILKHLADHMKPM 540

Query: 990  LNNSWQERRKAFFTENAEKMKRLLDSTQQKLDESFIDLQLYEKALDLFEDDQSISVVLHR 1049
            L NS +ERRK  FTENA++M+RL+D  Q+KLDESF+++QLYEKALDLFEDDQS +VVLHR
Sbjct: 541  LINSLKERRKKIFTENADRMRRLIDDLQKKLDESFLNMQLYEKALDLFEDDQSTAVVLHR 600

Query: 1050 HLLRTSAAPIVDMLFHNLDLYNKLKNGIEVAEMQNSEPVSLSTGERTTIAKSFPGSLSNK 1109
            HLLRT+AA I D L H LD++NK+KNG EV E +  + V L + ERT +AK+  GSLS K
Sbjct: 601  HLLRTTAATIADTLLHGLDIHNKMKNGTEVEESKTQDLVLLDSSERTALAKNLNGSLSKK 660

Query: 1110 AVTVAEALEGKRVETFMHALGDLVEESGMITKKLDKKLERTLLHSYRKNTMVIVWVRFLS 1169
            A+ + EALEGKRV+TFM    DL EESG++ KKLDKKLERTLLHSYRK            
Sbjct: 661  ALALVEALEGKRVDTFMVTFRDLAEESGLVLKKLDKKLERTLLHSYRK------------ 720

Query: 1170 RAAKRKVEMPLLSVILKNSGKMLLLTEAGRSLLPARLDLTSQVSAEMDPIALLPKVVSLL 1229
                                                 DL SQVS E DPIALL KVVSLL
Sbjct: 721  -------------------------------------DLISQVSTESDPIALLAKVVSLL 752

Query: 1230 YVQIHHKALQAPGRAISVAISRLKDKLDEPAYKILSDYQSATVTLLSLISAAVGD 1284
            +++IH+KALQAPGRAI+ AIS LK+KLDE AYK L+DYQ+ATVTLL+L+SA+ G+
Sbjct: 781  FIKIHNKALQAPGRAIAAAISHLKEKLDESAYKTLTDYQTATVTLLALMSASSGE 752

BLAST of CmaCh13G001460 vs. TAIR 10
Match: AT3G29635.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 317.4 bits (812), Expect = 5.4e-86
Identity = 187/460 (40.65%), Postives = 273/460 (59.35%), Query Frame = 0

Query: 6   SVKIIEICKVAPPPEASRAAAVPSSLPLTFFDVLWLRIYPIQRLFFYEL--PSNGISFID 65
           ++K+ +I +V+P   +S  +A    LPLTFFD+ WL+ +P +R+ FY+L   S+  SF+ 
Sbjct: 2   ALKVTKISQVSPASNSSNDSANSMVLPLTFFDLRWLQFHPTERVIFYKLIKDSSLESFLS 61

Query: 66  EVVPKLKTSLSLTLRHYLPLAGNLVWLPQSEVPTIEFVDGDGVSLTVAESGADFYQLSGN 125
            ++PKL+ SLS+ LRHYLPLAG L W  Q   P+I     D VSLTVAES ADF ++SG 
Sbjct: 62  VILPKLELSLSIVLRHYLPLAGRLTWSSQDPKPSIIVSPNDYVSLTVAESDADFSRISGK 121

Query: 126 GFREVSEFHPLVPQLPVSHDRAATIAIQVTIFQNKGFSIGITSHHAVVDGRTSTSFIKSW 185
           G R  SE   LVP+L +S D  + +++QVT+F N+GF IGI SHH+V+DG+T   FIKSW
Sbjct: 122 GIRPESEIRSLVPELSLSCDSPSVLSLQVTLFPNQGFCIGIASHHSVMDGKTVVRFIKSW 181

Query: 186 AQICMNESSVPIANLMPFYDRSVIDDPKGL-AKIY-ASAWMNQDGPDNRSLNLKLPK-TK 245
           A IC + +     +L P  DR+VI+ P  L AKI    ++ ++     RSL L  PK   
Sbjct: 182 AHICKHGAMDLPEDLTPVLDRTVINVPASLDAKIIELLSYFSEVKDSFRSLKLLPPKEIS 241

Query: 246 PSLIRSTFEFTHQNLEKLKKWVLKK-----NDEQMSSFVVTTAYLCVCTAKLDGLRDGN- 305
           P L+R + E T +N+EKL++   ++     ++  +S+FVV  AYL  C  K  G  +   
Sbjct: 242 PDLVRISLELTRENIEKLREQAKRESARSHHELHLSTFVVANAYLWTCLVKTRGGDENRP 301

Query: 306 LLFVFSGDVRSRLKPPVPLNYFGNCV--VGSFLSFERTELLGENGIALACEQISKATKNL 365
           + F+++ D R+RL PPVP  YFGNCV  +G F  ++    LGE+G     E +S + +++
Sbjct: 302 VRFMYAADFRNRLDPPVPEMYFGNCVFPIGCF-GYKANVFLGEDGFVNMVEILSDSVRSI 361

Query: 366 GGGGALKGAENLGTIVSEMTNDYSKIQP---IG-LAGSPRFGVYSADFGWGKPMKVEIVS 425
                  G   L TI     N    ++P   IG +AGS +FG+Y +DFGWGKP   EI S
Sbjct: 362 -------GLRKLETICELYINGTKSVKPGTQIGSIAGSNQFGLYGSDFGWGKPCNSEIAS 421

Query: 426 AESPLVFSLSDSRNSDVGMEIAVVKERDQMETFAALFNEG 449
            +    FS+S+ R+   G+EI +  ++ +M+ F  LF  G
Sbjct: 422 IDRNEAFSMSERRDEPGGVEIGLCLKKCEMDIFIYLFQNG 453

BLAST of CmaCh13G001460 vs. TAIR 10
Match: AT5G39050.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 308.5 bits (789), Expect = 2.5e-83
Identity = 177/465 (38.06%), Postives = 262/465 (56.34%), Query Frame = 0

Query: 6   SVKIIEICKVAPPPEASRAAAVPSSLPLTFFDVLWLRIYPIQRLFFYELPSNGISFIDEV 65
           S+K+I++ +V P    S  +    +LPLTFFD+LW +++ ++R+ FY+L      F D V
Sbjct: 9   SLKVIDVARVTPSNSDSSESL---TLPLTFFDLLWYKLHAVERVIFYKLTDASRPFFDSV 68

Query: 66  -VPKLKTSLSLTLRHYLPLAGNLVWLPQSEVPTIEFVDGDGVSLTVAESGADFYQLSGNG 125
            VP LKTSLS +L HYLPLAG LVW P    P I +   D VS TVAES ADF +L+G  
Sbjct: 69  IVPNLKTSLSSSLSHYLPLAGKLVWEPLDPKPKIVYTPNDAVSFTVAESNADFSRLTGKE 128

Query: 126 FREVSEFHPLVPQLPVSHDRAATIAIQVTIFQNKGFSIGITSHHAVVDGRTSTSFIKSWA 185
               +E +PLVP+L VS D A+ ++ QVT+F N+GF I + +HHAV+DG+T+T+F+KSWA
Sbjct: 129 PFPTTELYPLVPELHVSDDSASAVSFQVTLFPNQGFCISVNAHHAVLDGKTTTNFLKSWA 188

Query: 186 QICMNESSVPIANLMPFYDRSVIDDPKGLAKIYASAW-------MNQDGPDN-RSLNLK- 245
           + C N+ S    +L+P YDR+VI DP  L     +AW            P+N +SL L  
Sbjct: 189 RTCKNQDSFLPQDLIPVYDRTVIKDPMDLDTKILNAWHRVAKVFTGGKEPENPKSLKLLW 248

Query: 246 LPKTKPSLIRSTFEFTHQNLEKLKKWVLKKNDE----------QMSSFVVTTAYLCVCTA 305
            P+  P + R T   T ++++KL++ + K++            ++S+FV+  +Y   C  
Sbjct: 249 SPEIGPDVFRYTLNLTREDIQKLRERLKKESSSSSVSSSPKELRLSTFVIVYSYALTCLI 308

Query: 306 KLDGLRDGNLL-FVFSGDVRSRLKPPVPLNYFGNCVVGSF-LSFERTELLGENGIALACE 365
           K  G      + + F+ D RS + PPVP +YFGNCV   F +S      + E G   A  
Sbjct: 309 KARGGDPSRPVGYGFAVDCRSLMVPPVPSSYFGNCVSACFKMSLTAETFMSEEGFLAAAR 368

Query: 366 QISKATKNLGGGGALKGAENLGTIVSEMTNDYSKIQPIGLAGSPRFGVYSADFGWGKPMK 425
            +S + + L    ALK  E    I+   T      Q + +AGS RFGVY  DFGWG+P K
Sbjct: 369 MVSDSVEALDENVALKIPE----ILEGFTTLSPGTQVLSVAGSTRFGVYGLDFGWGRPEK 428

Query: 426 VEIVSAESPLVFSLSDSRNSDVGMEIAVVKERDQMETFAALFNEG 449
           V +VS +     S ++SR+   G+E+    ++ +M+    L ++G
Sbjct: 429 VVVVSIDQGEAISFAESRDGSGGVELGFSLKKHEMDVLVDLLHKG 466