CmaCh13G000220 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh13G000220 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.1e-74
Identity = 192/533 (36.02%), Postives = 293/533 (54.97%), Query Frame = 0

Query: 7   QWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFACVSGLAA 66
           +W  L+  IW+Q+  GTNF+F +YSS LK  L ISQ +LN LA ASD GK F   SG+A 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 67  NYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWINTVCYIV 126
            Y PL  VL   +++G VGYGVQ+L ITN    P Y L+F   +LAG SICW NT C+I+
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 127 AIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFPLGVC-- 186
            IR+FP+NR +A+ ++ S+ G+SA +Y+    L F+  +  ++  +LL NS+ PL V   
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALYS----LAFNAINPSSSNLYLLLNSLVPLVVSFA 222

Query: 187 ----VLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 246
               VL  P        + + +    F ++ V+ + T    +++S  + S  L+ +  +V
Sbjct: 223 ALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAVV 282

Query: 247 GILVFLLLPVVV-------PVVES---------VKKSVREGKERRVYHSTMEEDTRIRPE 306
            ++  L  P++V       PV+ +         V  ++ E K ++   S+      +   
Sbjct: 283 LLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGTA 342

Query: 307 NEAKLNRDGGDEAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSES 366
            E    R G + +        ++ R+ FWLY   YF G T+GL + NNLGQIA+S G  S
Sbjct: 343 KEGNTVRLGDEHSFR-----LLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNS 402

Query: 367 VSSLVSLSSSFGFFGRLMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFLL--SGPTD 426
            ++LV++ SSF FFGRL+ +  D    R ++  ++ GW    +      FFLL  S    
Sbjct: 403 -TTLVTIYSSFSFFGRLLSAAPDFMHKRFRL--TRTGWFAIALLPTPIAFFLLAVSSSQQ 462

Query: 427 LILCVSTAVIAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLY 486
             L  +TA+I + +G I + +VS TS+LFG N+  +NHN+++ NIP+GS L+GY+AA +Y
Sbjct: 463 TALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAASIY 522

Query: 487 RKQAT-------GGAGKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFY 509
              A+         +  CIG +CY  TF+ WGCL   G + +L L+ RT+  Y
Sbjct: 523 EANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of CmaCh13G000220 vs. ExPASy TrEMBL
Match: A0A6J1I3F0 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469568 PE=4 SV=1)

HSP 1 Score: 995.7 bits (2573), Expect = 7.1e-287
Identity = 520/520 (100.00%), Postives = 520/520 (100.00%), Query Frame = 0

Query: 1   MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC 60
           MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC
Sbjct: 1   MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC 60

Query: 61  VSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN 120
           VSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN
Sbjct: 61  VSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN 120

Query: 121 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP 180
           TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP
Sbjct: 121 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP 180

Query: 181 LGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 240
           LGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV
Sbjct: 181 LGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 240

Query: 241 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAMEE 300
           GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAMEE
Sbjct: 241 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAMEE 300

Query: 301 IGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSFGFFGR 360
           IGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSFGFFGR
Sbjct: 301 IGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSFGFFGR 360

Query: 361 LMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAVIAVCTGAIT 420
           LMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAVIAVCTGAIT
Sbjct: 361 LMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAVIAVCTGAIT 420

Query: 421 SISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGAGKCIGVECYR 480
           SISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGAGKCIGVECYR
Sbjct: 421 SISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGAGKCIGVECYR 480

Query: 481 TTFMIWGCLCSFGTLLALLLFARTRSFYSTTKSPRAQGST 521
           TTFMIWGCLCSFGTLLALLLFARTRSFYSTTKSPRAQGST
Sbjct: 481 TTFMIWGCLCSFGTLLALLLFARTRSFYSTTKSPRAQGST 520

BLAST of CmaCh13G000220 vs. ExPASy TrEMBL
Match: A0A6J1HMC6 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464885 PE=4 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 3.5e-278
Identity = 508/521 (97.50%), Postives = 511/521 (98.08%), Query Frame = 0

Query: 1   MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC 60
           MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSS LKHHLSISQFQLNNLAFASDAGKLFAC
Sbjct: 55  MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSPLKHHLSISQFQLNNLAFASDAGKLFAC 114

Query: 61  VSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN 120
           VSGLAANYLPLWS+LIIGS LGLVGYGVQYLFITNQFHSPSYCLIFFL+VLAGNSICWIN
Sbjct: 115 VSGLAANYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHSPSYCLIFFLSVLAGNSICWIN 174

Query: 121 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP 180
           TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP
Sbjct: 175 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP 234

Query: 181 LGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 240
           LGVCVLV PMARIVELVEE+GNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV
Sbjct: 235 LGVCVLVCPMARIVELVEEKGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 294

Query: 241 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAMEE 300
           GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAMEE
Sbjct: 295 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAMEE 354

Query: 301 IGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSFGFFGR 360
           IGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAES GSESVSSLVSLSSSFGFFGR
Sbjct: 355 IGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESWGSESVSSLVSLSSSFGFFGR 414

Query: 361 LMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGG-FFLLSGPTDLILCVSTAVIAVCTGAI 420
           LMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGG FFLLSGPTDLILCVSTAVIAVCTGAI
Sbjct: 415 LMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFFLLSGPTDLILCVSTAVIAVCTGAI 474

Query: 421 TSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGAGKCIGVECY 480
           TSISVSTTSELFGANNFSINHNVVVANIP GSFLFGYMAAYLYRKQATG  GKCIGVECY
Sbjct: 475 TSISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQATGDTGKCIGVECY 534

Query: 481 RTTFMIWGCLCSFGTLLALLLFARTRSFYSTTKSPRAQGST 521
           RTTFMIWGCLCS GTLLALLLFARTRSFYSTTKSP AQGST
Sbjct: 535 RTTFMIWGCLCSLGTLLALLLFARTRSFYSTTKSPMAQGST 575

BLAST of CmaCh13G000220 vs. ExPASy TrEMBL
Match: A0A1S3C569 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103497028 PE=4 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 7.7e-225
Identity = 424/522 (81.23%), Postives = 458/522 (87.74%), Query Frame = 0

Query: 1   MPSS--VFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLF 60
           MPSS  VFQWLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQ QLNNLAFASDAGKLF
Sbjct: 1   MPSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60

Query: 61  ACVSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICW 120
           AC SGLAANYLPLW VL IGSSLGL+GYGVQYLFITNQFHSPSY LIFFLTVLAGNSICW
Sbjct: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICW 120

Query: 121 INTVCYIVAIRNF-PSNRQVAVGISTSYQGLSAKIYTDIVGLIFS-KRSDKTAETFLLSN 180
           INTVCY+VAI NF  SNRQVAVGISTSYQGLSAK+YTDIVG IFS K S KTAETFLL N
Sbjct: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180

Query: 181 SVFPLGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPL 240
           SV PLGVCVLVSP+AR V++VEEQG LEVGFFVIFVITIATG FA +TSVGS+SRMLS L
Sbjct: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240

Query: 241 SGLVGILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRI----RPENEAKLNRD 300
           SGLVGI+VFL+LP+VV V E VK+ V EGKE RVYH T+EE+  +    R ENE K+ R 
Sbjct: 241 SGLVGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERT 300

Query: 301 GGDEAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLS 360
              EAMEEIGA EM+KR+NFWLY GVY FGATLGLAFLNNLGQIAESRGS SVSSLVSLS
Sbjct: 301 DEGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360

Query: 361 SSFGFFGRLMPSLLDHFLSRR-KMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAV 420
           SSFGFFGRL+PS+LD+FLSRR K MKSKPGW+V LMGA+CGGFFLL  P+D  LC+STA+
Sbjct: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAI 420

Query: 421 IAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGG-- 480
           IA+CTGAITSISVSTT++LFGA NFSINHNVVVANIP GSF+FGYMAA+LY KQA  G  
Sbjct: 421 IAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 480

Query: 481 AGKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYSTT 512
           AGKCIGVECYRTTF+IWGC  SFGT LALLLFARTRSFYSTT
Sbjct: 481 AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTT 522

BLAST of CmaCh13G000220 vs. ExPASy TrEMBL
Match: A0A0A0LYD4 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G533690 PE=4 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.8e-224
Identity = 421/523 (80.50%), Postives = 460/523 (87.95%), Query Frame = 0

Query: 1   MPSS---VFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKL 60
           MPSS   VFQWLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQ QLNNLAFASDAGKL
Sbjct: 1   MPSSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKL 60

Query: 61  FACVSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSIC 120
           FAC SGLAANYLPLW VL IGSSLGL+GYGVQYLFITNQFHSPSY LIFFLTVLAGNSIC
Sbjct: 61  FACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSIC 120

Query: 121 WINTVCYIVAIRNF-PSNRQVAVGISTSYQGLSAKIYTDIVGLIFS-KRSDKTAETFLLS 180
           WINTVCY+VAI NF  S+RQVAVGISTSYQGLSAK+YTDIVG IFS K S KTAETFLL 
Sbjct: 121 WINTVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLL 180

Query: 181 NSVFPLGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSP 240
           NSV PLGVCVLVSP+AR V++VEEQG LEVGFFVIFVITIATG+FA +TSVGS+SRMLS 
Sbjct: 181 NSVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSA 240

Query: 241 LSGLVGILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEE---DTRIRPENEAKLNRD 300
           L GLVGI+VFL+LP+VV VVE VK+ V EGKE +VYH T+EE   + R+R ENE K+ R 
Sbjct: 241 LGGLVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEERMRGENERKVERT 300

Query: 301 GGDEAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLS 360
              EAMEEIGA EM+KR+NFWLYVGVY FGATLGLAFLNNLGQIAESRGS SVSSLVSLS
Sbjct: 301 DDGEAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360

Query: 361 SSFGFFGRLMPSLLDHFLSRR-KMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAV 420
           SSFGFFGRL+PS+LD+FLSRR K MKSKPGW+V LMG +CGGFFLL  P+D  LC+STA+
Sbjct: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAI 420

Query: 421 IAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGA- 480
           IA+CTGAITSISVSTT++LFGA NFSINHN+VVANIP GSF+FGYMAA+LYRKQA  G  
Sbjct: 421 IAICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYRKQAGHGVD 480

Query: 481 -GKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYSTTK 513
            GKCIGVECYRTTF+IWG   SFGT LALLLFART+SFYSTTK
Sbjct: 481 PGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTK 523

BLAST of CmaCh13G000220 vs. ExPASy TrEMBL
Match: A0A5D3BBQ0 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00460 PE=4 SV=1)

HSP 1 Score: 745.0 bits (1922), Expect = 2.2e-211
Identity = 408/522 (78.16%), Postives = 438/522 (83.91%), Query Frame = 0

Query: 1   MPSS--VFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLF 60
           MPSS  VFQWLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQ QLNNLAFASDAGKLF
Sbjct: 1   MPSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60

Query: 61  ACVSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICW 120
           AC SGLAANYLPLW VL IGSSLGL+GYGVQYLFITNQFHSPSY LIFFLTVLAGNSICW
Sbjct: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICW 120

Query: 121 INTVCYIVAIRNF-PSNRQVAVGISTSYQGLSAKIYTDIVGLIFS-KRSDKTAETFLLSN 180
           INTVCY+VAI NF  SNRQVAVGISTSYQGLSAK+YTDIVG IFS K S KTAETFLL N
Sbjct: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180

Query: 181 SVFPLGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPL 240
           SV PLGVCVLVSP+AR V++VEEQG LEVGFFVIFVITIATG FA +TSVGS+SRMLS L
Sbjct: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240

Query: 241 SGLVGILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRI----RPENEAKLNRD 300
           SGLVGI+VFL+LP+VV V E VK+ V EGKE RVYH T+EE+  +    R ENE K+ R 
Sbjct: 241 SGLVGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERT 300

Query: 301 GGDEAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLS 360
              EAMEEIGA EM+KR+NFWLY GVY FGATLGLAFLNNLGQIAESRGS SVSSLVSLS
Sbjct: 301 DEGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360

Query: 361 SSFGFFGRLMPSLLDHFLSRR-KMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAV 420
           SSFGFFGRL+PS+LD+FLSRR K MKSKPGW+V LMGA+CG                   
Sbjct: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCG------------------- 420

Query: 421 IAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGG-- 480
                GAITSISVSTT++LFGA NFSINHNVVVANIP GSF+FGYMAA+LY KQA  G  
Sbjct: 421 -----GAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 480

Query: 481 AGKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYSTT 512
           AGKCIGVECYRTTF+IWGC  SFGT LALLLFARTRSFYSTT
Sbjct: 481 AGKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTT 498

BLAST of CmaCh13G000220 vs. NCBI nr
Match: XP_022970645.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 995.7 bits (2573), Expect = 1.5e-286
Identity = 520/520 (100.00%), Postives = 520/520 (100.00%), Query Frame = 0

Query: 1   MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC 60
           MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC
Sbjct: 1   MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC 60

Query: 61  VSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN 120
           VSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN
Sbjct: 61  VSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN 120

Query: 121 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP 180
           TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP
Sbjct: 121 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP 180

Query: 181 LGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 240
           LGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV
Sbjct: 181 LGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 240

Query: 241 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAMEE 300
           GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAMEE
Sbjct: 241 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAMEE 300

Query: 301 IGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSFGFFGR 360
           IGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSFGFFGR
Sbjct: 301 IGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSFGFFGR 360

Query: 361 LMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAVIAVCTGAIT 420
           LMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAVIAVCTGAIT
Sbjct: 361 LMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAVIAVCTGAIT 420

Query: 421 SISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGAGKCIGVECYR 480
           SISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGAGKCIGVECYR
Sbjct: 421 SISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGAGKCIGVECYR 480

Query: 481 TTFMIWGCLCSFGTLLALLLFARTRSFYSTTKSPRAQGST 521
           TTFMIWGCLCSFGTLLALLLFARTRSFYSTTKSPRAQGST
Sbjct: 481 TTFMIWGCLCSFGTLLALLLFARTRSFYSTTKSPRAQGST 520

BLAST of CmaCh13G000220 vs. NCBI nr
Match: KAG6583327.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019101.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 974.5 bits (2518), Expect = 3.5e-280
Identity = 511/521 (98.08%), Postives = 514/521 (98.66%), Query Frame = 0

Query: 1   MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC 60
           MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC
Sbjct: 1   MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC 60

Query: 61  VSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN 120
           VSGLAANYLPLWS+LIIGS LGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN
Sbjct: 61  VSGLAANYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN 120

Query: 121 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP 180
           TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP
Sbjct: 121 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP 180

Query: 181 LGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 240
           LGVCVLV PMARIVELVEE+GNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV
Sbjct: 181 LGVCVLVCPMARIVELVEEKGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 240

Query: 241 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAMEE 300
           GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAMEE
Sbjct: 241 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAMEE 300

Query: 301 IGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSFGFFGR 360
           IGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSES SSLVSLSSSFGFFGR
Sbjct: 301 IGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESASSLVSLSSSFGFFGR 360

Query: 361 LMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGG-FFLLSGPTDLILCVSTAVIAVCTGAI 420
           LMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGG FFLLSGPTDLILCVSTAVIAVCTGAI
Sbjct: 361 LMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFFLLSGPTDLILCVSTAVIAVCTGAI 420

Query: 421 TSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGAGKCIGVECY 480
           TSISVSTTSELFGANNFSINHNVVVANIP GSFLFGYMAAYLYRKQA G AGKCIGVECY
Sbjct: 421 TSISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQARGDAGKCIGVECY 480

Query: 481 RTTFMIWGCLCSFGTLLALLLFARTRSFYSTTKSPRAQGST 521
           RT+FMIWGCLCSFGTLLALLLFARTRSFYSTTKSPRAQGST
Sbjct: 481 RTSFMIWGCLCSFGTLLALLLFARTRSFYSTTKSPRAQGST 521

BLAST of CmaCh13G000220 vs. NCBI nr
Match: XP_022964928.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 966.8 bits (2498), Expect = 7.3e-278
Identity = 508/521 (97.50%), Postives = 511/521 (98.08%), Query Frame = 0

Query: 1   MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC 60
           MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSS LKHHLSISQFQLNNLAFASDAGKLFAC
Sbjct: 55  MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSPLKHHLSISQFQLNNLAFASDAGKLFAC 114

Query: 61  VSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN 120
           VSGLAANYLPLWS+LIIGS LGLVGYGVQYLFITNQFHSPSYCLIFFL+VLAGNSICWIN
Sbjct: 115 VSGLAANYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHSPSYCLIFFLSVLAGNSICWIN 174

Query: 121 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP 180
           TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP
Sbjct: 175 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP 234

Query: 181 LGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 240
           LGVCVLV PMARIVELVEE+GNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV
Sbjct: 235 LGVCVLVCPMARIVELVEEKGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 294

Query: 241 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAMEE 300
           GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAMEE
Sbjct: 295 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAMEE 354

Query: 301 IGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSFGFFGR 360
           IGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAES GSESVSSLVSLSSSFGFFGR
Sbjct: 355 IGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESWGSESVSSLVSLSSSFGFFGR 414

Query: 361 LMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGG-FFLLSGPTDLILCVSTAVIAVCTGAI 420
           LMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGG FFLLSGPTDLILCVSTAVIAVCTGAI
Sbjct: 415 LMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFFLLSGPTDLILCVSTAVIAVCTGAI 474

Query: 421 TSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGAGKCIGVECY 480
           TSISVSTTSELFGANNFSINHNVVVANIP GSFLFGYMAAYLYRKQATG  GKCIGVECY
Sbjct: 475 TSISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQATGDTGKCIGVECY 534

Query: 481 RTTFMIWGCLCSFGTLLALLLFARTRSFYSTTKSPRAQGST 521
           RTTFMIWGCLCS GTLLALLLFARTRSFYSTTKSP AQGST
Sbjct: 535 RTTFMIWGCLCSLGTLLALLLFARTRSFYSTTKSPMAQGST 575

BLAST of CmaCh13G000220 vs. NCBI nr
Match: XP_023519273.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 962.2 bits (2486), Expect = 1.8e-276
Identity = 504/521 (96.74%), Postives = 510/521 (97.89%), Query Frame = 0

Query: 1   MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC 60
           MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC
Sbjct: 1   MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC 60

Query: 61  VSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN 120
           VSGLAANYLPLWS+LIIGS LGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN
Sbjct: 61  VSGLAANYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN 120

Query: 121 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP 180
           TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIF+KRSDKTAETFLLSNSVFP
Sbjct: 121 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFTKRSDKTAETFLLSNSVFP 180

Query: 181 LGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 240
           LGVCVLV PMARIVELVEE+GNL VGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV
Sbjct: 181 LGVCVLVCPMARIVELVEEKGNLGVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 240

Query: 241 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAMEE 300
           GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGD+AMEE
Sbjct: 241 GILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEEDTRIRPENEAKLNRDGGDDAMEE 300

Query: 301 IGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSFGFFGR 360
           IGA EM+ RVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSFGFFGR
Sbjct: 301 IGAREMVTRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSFGFFGR 360

Query: 361 LMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGG-FFLLSGPTDLILCVSTAVIAVCTGAI 420
           LMPSLLDHFLSRRKMMKSKPGW+V LMGAMCGG FFLLSGPTDLILCVSTAVIAVCTGAI
Sbjct: 361 LMPSLLDHFLSRRKMMKSKPGWMVGLMGAMCGGFFFLLSGPTDLILCVSTAVIAVCTGAI 420

Query: 421 TSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGAGKCIGVECY 480
           TSISVSTTSELFGANNFSINHNVVVANIP GSFLFGYMAAYLYRKQATGGAGKCIGVECY
Sbjct: 421 TSISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQATGGAGKCIGVECY 480

Query: 481 RTTFMIWGCLCSFGTLLALLLFARTRSFYSTTKSPRAQGST 521
           RTTFMIWGCLCSFGTLLALLLFARTRSFYSTTKS  A GST
Sbjct: 481 RTTFMIWGCLCSFGTLLALLLFARTRSFYSTTKSQMAHGST 521

BLAST of CmaCh13G000220 vs. NCBI nr
Match: XP_038895111.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Benincasa hispida])

HSP 1 Score: 810.1 bits (2091), Expect = 1.1e-230
Identity = 423/516 (81.98%), Postives = 465/516 (90.12%), Query Frame = 0

Query: 3   SSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFACVS 62
           S VFQWLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQ QLNNLAFASDAGKLFAC S
Sbjct: 4   SIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFS 63

Query: 63  GLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWINTV 122
           GLAANYLPLW VL IG+SLGLVGYGVQYLF+TNQ  SPSYC+IF LTVLAGNSICWINTV
Sbjct: 64  GLAANYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQSPSYCVIFLLTVLAGNSICWINTV 123

Query: 123 CYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFPLG 182
           CY+VAI NF +NRQ+AVGISTSYQGLSAKIYT+IV  IFSK S KT ETFLL NSV P+G
Sbjct: 124 CYMVAITNFGTNRQIAVGISTSYQGLSAKIYTNIVSSIFSKHSSKTTETFLLLNSVLPMG 183

Query: 183 VCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLVGI 242
           VCVL SP+ARI+++VEEQG LEVGFFVIFVITIATG+FA VTSVGSISRMLSPLSGLVGI
Sbjct: 184 VCVLASPLARILKVVEEQGKLEVGFFVIFVITIATGIFAAVTSVGSISRMLSPLSGLVGI 243

Query: 243 LVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED---TRIRPENEAKLNRDG-GDEAM 302
           +VFL+LP+VV VVE VK+S++EGKERRVYH T+EE+    R+R EN++KL RDG  +EAM
Sbjct: 244 VVFLVLPLVVAVVEKVKESLKEGKERRVYHFTVEENKDVERVRGENQSKLERDGDSEEAM 303

Query: 303 EEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSFGFF 362
           EEIGA EM+KR+NFWLY GVY FGATLGLAFLNNLGQIAESRGS SVSSLVSLSSSFGFF
Sbjct: 304 EEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSFGFF 363

Query: 363 GRLMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAVIAVCTGA 422
           GRLMPS+LD+FLSR+K MKSKPGW+V LMG +CGGFFLL  PTD+ LCVSTA+IAVCTGA
Sbjct: 364 GRLMPSILDYFLSRKKCMKSKPGWMVGLMGVLCGGFFLLLCPTDVSLCVSTAIIAVCTGA 423

Query: 423 ITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATG-GAGKCIGVE 482
           ITSISVSTTSELFGANNFSINHN++VANIP GSFLFGYMAA+LY KQA G G GKCIGVE
Sbjct: 424 ITSISVSTTSELFGANNFSINHNLLVANIPFGSFLFGYMAAFLYHKQAGGDGTGKCIGVE 483

Query: 483 CYRTTFMIWGCLCSFGTLLALLLFARTRSFYSTTKS 514
           CYRTTF IWGC  SFGTLLALLLFART++FYSTT+S
Sbjct: 484 CYRTTFFIWGCFSSFGTLLALLLFARTKTFYSTTRS 519

BLAST of CmaCh13G000220 vs. TAIR 10
Match: AT2G30300.1 (Major facilitator superfamily protein )

HSP 1 Score: 433.0 bits (1112), Expect = 3.5e-121
Identity = 250/522 (47.89%), Postives = 333/522 (63.79%), Query Frame = 0

Query: 1   MPSSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFAC 60
           M   +  WLSL+ I+WLQS NGTN +FP+YSSQLK  L ISQF+LN L+FASDAGK+   
Sbjct: 1   MSRKILPWLSLVAILWLQSFNGTNLSFPAYSSQLKELLKISQFKLNYLSFASDAGKVLGF 60

Query: 61  VSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWIN 120
           +SG+AA YLPL  VL+ G SLG  GYG+QYL I  +                   ICWIN
Sbjct: 61  ISGIAAVYLPLPLVLLAGGSLGFAGYGLQYLSIIKKI------------------ICWIN 120

Query: 121 TVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFP 180
           T CYIVAI +FP NRQVAVGI+ SYQGLS KIYTD+V   F     + A  +LL NS+ P
Sbjct: 121 TACYIVAINSFPVNRQVAVGITASYQGLSGKIYTDMVHSFFHTSQREEASGYLLLNSLVP 180

Query: 181 LGVCVLVSPMARI---VELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLS 240
           L  C++ +PM       + +    +++VGF V+FV+TIATG++AV TS+ S+  +L    
Sbjct: 181 LVACLVTAPMLMRHGGDKTMSYSKDVKVGFIVLFVLTIATGIYAVATSLVSVPAVLV--- 240

Query: 241 GLVGILVFLLLPVVVPVVESVKKSVREGKERRVYHST--------MEEDTRIRPENEAKL 300
            LVGI +FLL P+ +P+    K+ +   K ++  H            E+   + E E + 
Sbjct: 241 -LVGIALFLLAPLAIPIGVGFKELMSSRKTQQKVHDLEAPVDKFYFVEEDHTKEEEEFEK 300

Query: 301 NRDGGDEAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLV 360
              G     EE+   ++ K+++FW+Y G+Y FG T+GL F NNLGQIAESRGS + SSLV
Sbjct: 301 AIIG---VKEEVEWTQLWKKLDFWIYFGLYLFGPTVGLVFTNNLGQIAESRGSTATSSLV 360

Query: 361 SLSSSFGFFGRLMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVST 420
           +LSSSFGFFGRL+PSLLD+F SR K M S P  +   + AM   F LL   +D+ L + T
Sbjct: 361 ALSSSFGFFGRLLPSLLDYFFSRNKYMPSSPVSMAGSLVAMVASFLLLLIDSDIALYIGT 420

Query: 421 AVIAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQAT-- 480
           A+I + +GA+TS+SV+ T+ELFG  +F +NHN+VV +IP+GSF FG +AA +YR  A   
Sbjct: 421 AMIGIFSGALTSLSVTMTAELFGTKHFGVNHNIVVGSIPLGSFSFGLLAAKIYRDGAALY 480

Query: 481 GGAGKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYS 510
           G  GKC G+ C++TT + WG LCS   LLA +L+ R R FYS
Sbjct: 481 GDDGKCFGMHCFQTTLVFWGMLCSIAALLAAVLYIRNRKFYS 497

BLAST of CmaCh13G000220 vs. TAIR 10
Match: AT5G45275.1 (Major facilitator superfamily protein )

HSP 1 Score: 292.0 bits (746), Expect = 9.7e-79
Identity = 209/572 (36.54%), Postives = 313/572 (54.72%), Query Frame = 0

Query: 7   QWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFACVSGLAA 66
           +W+ L+  IW+Q+  GTNF+F +YSS LK  L ISQ QLN LA ASD GK+F   SGLA 
Sbjct: 7   KWMILVATIWIQAFTGTNFDFSTYSSNLKSVLGISQVQLNYLAVASDLGKVFGWSSGLAL 66

Query: 67  NYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWINTVCYIV 126
            Y PLW+VL   + +G VGYGVQ+L ITN    P Y L+F   +LAG SICW NTVC+++
Sbjct: 67  LYFPLWTVLFAAAIMGFVGYGVQWLVITNVISLP-YILVFLCCLLAGLSICWFNTVCFVL 126

Query: 127 AIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFPLGVC-V 186
            IRNFP+NR +A+ ++ S+ G+SA +YT    L ++  +  + E +LL N++ PL V   
Sbjct: 127 CIRNFPANRSLALSLTVSFNGVSAALYT----LAYNAINPVSTELYLLLNALVPLFVSFA 186

Query: 187 LVSPMAR--IVELVEEQG--NLEVGFFVIFVITIATGVFAVV----TSVGSISRMLSPLS 246
            + P+ R   +E +   G     + F ++ ++ +  GV+ ++    TS  + +R+L    
Sbjct: 187 ALIPILRQPPLEPLPPDGVRRDSLMFLLLNILAVLNGVYLLLFRSKTSDVTSARLL---- 246

Query: 247 GLVGILVFLLLPVVVP--------VVESVKKSVR-EG-------------------KERR 306
              G ++ L+LP+ +P         + ++  S R EG                   +E  
Sbjct: 247 -FGGSILLLILPLCLPGLVYARNWYLHNIHSSFRLEGSGFILVDVDELEMHKGMVTREAS 306

Query: 307 VYHSTMEEDTRIR----PENEAKLNRDGG------------DEAMEEIGAMEMLKRVNFW 366
           +    +  D  +R    P+ ++ +  D G                EE     +L R +FW
Sbjct: 307 LEGYQLLNDDVVRAVNTPDQKSFIEDDDGCCCTKVITRNQLGMLGEEHPLSFLLCRSDFW 366

Query: 367 LYVGVYFFGATLGLAFLNNLGQIAESRG-SESVSSLVSLSSSFGFFGRLMPSLLDHFLSR 426
           LY   YF G T+GL + NNLGQIA+S G S   ++LV+L SSF FFGRL+ +  D+   R
Sbjct: 367 LYYIAYFCGGTIGLVYSNNLGQIAQSLGQSSETTTLVTLYSSFSFFGRLLSATPDYI--R 426

Query: 427 RKMMKSKPGWL-VALMGAMCGGFFLLSGPTDLILCVSTAVIAVCTGAITSISVSTTSELF 486
            K+  ++ GWL VAL+      F L S  +   L   TA+I + +G I + +VS TSELF
Sbjct: 427 AKVYFARTGWLAVALLPTTIALFLLASSGSLAALQAGTALIGLSSGFIFAAAVSITSELF 486

Query: 487 GANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGAGK----CIGVECYRTTFMIWG 520
           G N+  +NHN+++ NIP+GS ++G++AA +Y   +  G+      C+G +CY  TFM WG
Sbjct: 487 GPNSVGVNHNILITNIPIGSLVYGFLAALVYESHSVAGSKTESVICMGRDCYLQTFMWWG 546

BLAST of CmaCh13G000220 vs. TAIR 10
Match: AT4G19450.1 (Major facilitator superfamily protein )

HSP 1 Score: 287.7 bits (735), Expect = 1.8e-77
Identity = 200/566 (35.34%), Postives = 307/566 (54.24%), Query Frame = 0

Query: 7   QWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFACVSGLAA 66
           +W+ L+  IW+Q+  GTNF+F +YSS LK  L ISQ QLN LA ASD GK+F   SGLA 
Sbjct: 7   KWMILVATIWIQAFTGTNFDFSAYSSDLKSVLGISQVQLNYLAVASDLGKVFGWSSGLAL 66

Query: 67  NYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWINTVCYIV 126
            Y PLW+VL   + +G VGYGVQ+L IT+ F S  Y ++F   +LAG SICW NTVC+++
Sbjct: 67  MYFPLWTVLFAAAFMGFVGYGVQWLVITH-FISLPYIMVFLCCLLAGLSICWFNTVCFVL 126

Query: 127 AIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFPLGVC-V 186
            I NFP+NR +A+ ++ S+ G+SA +YT    L ++  +  + E +LL N++ PL V   
Sbjct: 127 CISNFPANRSLALSLTVSFNGVSAALYT----LAYNAINPTSPELYLLLNALIPLIVSFT 186

Query: 187 LVSPMAR--IVELVEEQG--NLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLVG 246
            + P+ R    E +   G     + F ++ ++    GV+ ++    S S + S      G
Sbjct: 187 AIIPILRQPPFEPLPPDGVRRDSLMFLLLNILAALNGVYLLLFGSNS-SDLTSARLLFGG 246

Query: 247 ILVFLLLPVVVP-------------------------VVESVKKSVREG----KERRVYH 306
            ++ L+ P+ +P                         +V+  +  + +G    +  R  +
Sbjct: 247 AILLLVFPLCIPGLVIARNWYNRTIHTSFRLEGSGFILVDPDELELHKGMLAHEANREGY 306

Query: 307 STMEEDTRIRPENEAKLNRDGGDEA----------MEEIGAME----MLKRVNFWLYVGV 366
             + +D    P     +  +  DE+          +E +G       +L R +FWLY   
Sbjct: 307 QLLSDDVVQNPVKSVAVEEEDSDESCCKKLITRDQLEGLGIEHSLSLLLTRSDFWLYYIT 366

Query: 367 YFFGATLGLAFLNNLGQIAESRG-SESVSSLVSLSSSFGFFGRLMPSLLDHFLSRRKMMK 426
           YF G T+GL + NNLGQIA+S G S + ++LV+L S+F FFGRL+ +  D+   R K+  
Sbjct: 367 YFCGGTIGLVYSNNLGQIAQSLGQSSNTTTLVTLYSAFSFFGRLLSATPDYI--RAKVYF 426

Query: 427 SKPGWL-VALMGAMCGGFFLLSGPTDLILCVSTAVIAVCTGAITSISVSTTSELFGANNF 486
           ++ GWL +AL+      F L S  T   L   TA++ + +G I + +VS TSELFG N+ 
Sbjct: 427 ARTGWLAIALLPTPFALFLLASSGTASALQAGTALMGLSSGFIFAAAVSITSELFGPNSV 486

Query: 487 SINHNVVVANIPVGSFLFGYMAAYLY-------RKQATGGAGKCIGVECYRTTFMIWGCL 516
            +NHN+++ NIP+GS ++G++AA +Y        K  T  +  C+G +CY  TF+ WGCL
Sbjct: 487 GVNHNILITNIPIGSLIYGFLAALVYDSHGFTGTKSMTSESVVCMGRDCYYLTFVWWGCL 546

BLAST of CmaCh13G000220 vs. TAIR 10
Match: AT1G31470.1 (Major facilitator superfamily protein )

HSP 1 Score: 282.3 bits (721), Expect = 7.7e-76
Identity = 192/533 (36.02%), Postives = 293/533 (54.97%), Query Frame = 0

Query: 7   QWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFACVSGLAA 66
           +W  L+  IW+Q+  GTNF+F +YSS LK  L ISQ +LN LA ASD GK F   SG+A 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 67  NYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWINTVCYIV 126
            Y PL  VL   +++G VGYGVQ+L ITN    P Y L+F   +LAG SICW NT C+I+
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 127 AIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFPLGVC-- 186
            IR+FP+NR +A+ ++ S+ G+SA +Y+    L F+  +  ++  +LL NS+ PL V   
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALYS----LAFNAINPSSSNLYLLLNSLVPLVVSFA 222

Query: 187 ----VLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLV 246
               VL  P        + + +    F ++ V+ + T    +++S  + S  L+ +  +V
Sbjct: 223 ALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAVV 282

Query: 247 GILVFLLLPVVV-------PVVES---------VKKSVREGKERRVYHSTMEEDTRIRPE 306
            ++  L  P++V       PV+ +         V  ++ E K ++   S+      +   
Sbjct: 283 LLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGTA 342

Query: 307 NEAKLNRDGGDEAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSES 366
            E    R G + +        ++ R+ FWLY   YF G T+GL + NNLGQIA+S G  S
Sbjct: 343 KEGNTVRLGDEHSFR-----LLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNS 402

Query: 367 VSSLVSLSSSFGFFGRLMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFLL--SGPTD 426
            ++LV++ SSF FFGRL+ +  D    R ++  ++ GW    +      FFLL  S    
Sbjct: 403 -TTLVTIYSSFSFFGRLLSAAPDFMHKRFRL--TRTGWFAIALLPTPIAFFLLAVSSSQQ 462

Query: 427 LILCVSTAVIAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLY 486
             L  +TA+I + +G I + +VS TS+LFG N+  +NHN+++ NIP+GS L+GY+AA +Y
Sbjct: 463 TALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAASIY 522

Query: 487 RKQAT-------GGAGKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFY 509
              A+         +  CIG +CY  TF+ WGCL   G + +L L+ RT+  Y
Sbjct: 523 EANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of CmaCh13G000220 vs. TAIR 10
Match: AT3G01630.1 (Major facilitator superfamily protein )

HSP 1 Score: 275.8 bits (704), Expect = 7.2e-74
Identity = 189/532 (35.53%), Postives = 290/532 (54.51%), Query Frame = 0

Query: 7   QWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFACVSGLAA 66
           +W  L+  IW+Q+  GTNF+F +YSS +K  + +SQ +LN +A ASD GK     SG A 
Sbjct: 23  RWTVLVAAIWIQAFTGTNFDFSAYSSDMKSSMGVSQSRLNYMAVASDLGKALGWSSGFAI 82

Query: 67  NYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWINTVCYIV 126
            Y P+  VL   +++GLVGYGVQ+L I +    P Y L+     LAG SICW NTV +I+
Sbjct: 83  AYFPVSGVLFAAAAMGLVGYGVQWLSIADVIDLP-YSLVLVCCSLAGLSICWFNTVSFIL 142

Query: 127 AIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFPLGVCVL 186
            IR+F +N  +A+ +  S+ G+SA +YT +     S +S  +++ +LL NS+ PL V VL
Sbjct: 143 CIRHFKANHSLALSLVVSFNGISAALYT-LGHEAISGKSSASSDIYLLLNSLIPLIVSVL 202

Query: 187 ----VSPMARIVELVEEQGNLEVGFFVIF-VITIATGVFAVVTSVGSISRMLSPLSGLVG 246
               V       E    + + E   FV+F V+ + T  + ++ S G+     SP    +G
Sbjct: 203 ALWPVLTNPNSSETDTTRTHDETRIFVVFNVLALVTCFYLLLPSSGTYLAS-SPRWHFLG 262

Query: 247 ILVFLLLPVVVPVVESVKKSVRE--GKERRVYHSTMEEDTRIRPENEAKLNRDGGDEAM- 306
            +  LL P+ VP ++ + +++          Y +   E+ +I      K N +   + + 
Sbjct: 263 AIFLLLFPLCVPFLDYIHRALESCFHHHSSGYAAVNIEEPKILKIKSQKSNAEEESDQVR 322

Query: 307 --EEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRG--SESVSSLVSLSSS 366
             +E     +++++ FWLY   YF G T+GL + NNLGQIA+S G  S +  SLV+L S+
Sbjct: 323 LGDEHSLGMLVRKLEFWLYYVAYFCGGTIGLVYSNNLGQIAQSLGQSSSNAKSLVTLFSA 382

Query: 367 FGFFGRLMPSLLDHFLSRRKMMKSKPGWL-VALMGAMCGGFFLLSGP---TDLILCVSTA 426
           F F GRL+ S  D F  ++    ++ GW  ++L+      F L   P      +L V+TA
Sbjct: 383 FSFLGRLLSSAPD-FTRKKLKYLTRTGWFTISLLPTPLAFFILAYSPKTNQTALLEVATA 442

Query: 427 VIAVCTGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLY-------R 486
           +I + +G + + +VS TSELFG N+  +N N+++ NIP+GS  +GYMA  +Y       R
Sbjct: 443 LIGLSSGFVFAAAVSITSELFGRNSVGVNQNILITNIPIGSLFYGYMAGSVYDTNASLGR 502

Query: 487 KQATGGAGKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYSTTKSPR 516
           K     +  C+G +CY  TF+ WGCL   G + +L LF RTR  Y   K  R
Sbjct: 503 KSVVADSVVCVGRKCYFVTFLFWGCLSVLGFVCSLFLFIRTRPVYHRLKLNR 550