CmaCh12G013330 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G013330
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionpollen receptor-like kinase 3
LocationCma_Chr12: 10144846 .. 10147301 (-)
RNA-Seq ExpressionCmaCh12G013330
SyntenyCmaCh12G013330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAGAAGCTCTGCCTCACACACTCCAGAGACAACCACGCAATTCTTCGAACAGCGGCTGGTTTCTACCCGGAGCTATTCCATTTCCCGTTCCAGCTCTGCAATGGCCGCTGTTCGAAATGTGTTCCTCTTCCTCACCATCTTCCTCGTCGGCGGTCCACCTCTTTCCTGTTCCCTCTCCGATAACGAGGCCCTCCTGAAGCTCAGGCAATCCTTCACCCATGCCGAATCCCTTAATTCCTGGGACCCGGACTCCCAACCCTGCGCCGCTCGATGGACTGGCATCATCTGCCACAGAGGCGTCATCACCGGTCTCCATCTCACCGGCCTCCGACTCTCCGGCCAGATCGACATCGAAGCTCTCCTCCAGCTTCGCAGCCTTCGAACCGTCAGCCTCGTCAACAACAGCTTCGCCGGGCCGATTCCCGAGTTCAACAAAATCGGCGTTCTCAAATCCCTCCTTCTCACAGGGAATGATTTCTCCGGGGCTATTCCTACCGATTTCTTCGCATCCCTCGCCTCTCTCAAGAAGGTTTGGCTCTCTTCCAACAATTTCTCCGGCGACATTCCGCACTCTCTGGCGAAACTCTCGTATCTAATCGAACTCCATCTCGAAAACAACCAATTCTCCGGCCAAATCCCTCACCTACACCAGGCCGCCTTAACCTCTCTAGATCTCTCCAATAACAAGCTGGAGGGAGAAATTCCAGAGAATCTAGCGAAGTTCGACGCGAACGCATTTGCCGGAAACGAAGGTCTGTGTGGGAAGCCGCTCCCGAAATCATGCGACGGCAATAACGCGCAGATTTCGGAGGAAAACGCGTCTTCTCCGGGGGAATCACAAGGCAGCGTCTCGAAATTAGTAGTGGCGTCGTTGATAGCGGTGGCTGTATTTCTGGTGGTGGTTATGTTTCTGAGCGCGTCGAAGCGGAGAGAGGAGAAGTTCAGCAAGCTAGGGCGGGAGCAGATGAATGAGATGGTGGAGGTGCACGTGCCGAGTTCCGGGCATAGGGAGCAGCTGAGTAGAAAAGGTGGGGATTCCAGGAGAGGGTCGCAACAGGGGAAGGGAGGGATGAGTGATCTTGTGGTAGTGAATGAAGAAAAGGGTACGTTTGGGATGGCGGATTTGATGAAGGCGGCGGCGGAGGTGCTGGGGAATGGTGGGTTGGGGTCGGCTTACAAGGCCGTCATGGCAAATGGGCTGTCGGTGGTGGTGAAAAGAATCAGAGAAATGAACAAATTAGGGAAGGATGGGTTCGATGCAGAGATGAGGCGCATGGGACGGCTGCAGCATCACAATATTCTCACGCCTTTGGCGTACCATTACAGGCGGGAGGAGAAGCTGCTGGTGTCCGAGTACATTCCTAAAGGCAGCCTCTTGTATGTGTTGCATGGTACGTTTCTTAATTGGATTGAATTACAAAAAAAAAAAAATAATAATAATAAATAAATAAATAAAAATCTTAGAACAAGTTAATAATCACAAATTCTCTCTAGAACAATCTTTACTGAATTGAGACTCTTAAACAAAGTACACCCTTCTATTCAATACTTTAGTCATTTATTTTTTATTCATTGAGGCTCACAATTCTGTGTTACTCTTAATACTATGTTAAGAATTATTGATCCCCTAAACAGGAACCTGGAACCTAAGCTCTCATATATTTGTTTTGTATTTCTCAAAAGACATAAATCTGAGATTCCTCCAACCATCCTAAAGAATCCTGAACCGAACATAATAATCGAGCAGGGGACCGTGGAGCCTGCCATGCGGAGCTTAACTGGGGCACCCGGTTAAAGATCGTGAAGGGGATCGCCAGAGGCCTAGGGTTTCTCCATTCCGAGTTCGCGACCTACGATCTGCCGCATGGGAATCTGAAATCAAGCAACATTCTTCTAAGCGACACCTACGAGCCACTTCTCAGCGACTACGCGTTTCATCCGCTAATCAATCCAAACAACGCGACGGAGGCAATGTTTGCGTACAAATCCCCGGAGTACGCGCATCGGCAGGAAGTTTCTCCAAAATCTGATGTTTATTGCCTTGGAATTATCATTCTAGAAATTCTGACCGGCAAATTTCCTTCTCAATATCTTAGCAATGGCAAGGGAGGGACCGATGTGGTGCAGTGGGTCTCCTCCGCAGTAGCCGAGAAGAGGGAGGCGGAACTCATAGACCCTGAGATAGCAAACGACGCGAATGCAGTGAGTCGGATGGTCGAGCTCCTTAGAATCGGCGCCGATTGCACCCAATCCAATCCTCAGCAACGGCCAGAAATGAAGGAAGCCATTAGGAGAATAGANGAAATATGAGGAATCATAAATTTGTTTTTGGAAACTAAGAAAAGGGCATATTGAGGGGGGGGAGGGGGGAGGGGGGATGTGGGGAAGAACACAAAAAACTAATGTGGTAGATGGATAG

mRNA sequence

AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGAAAAGAAGCTCTGCCTCACACACTCCAGAGACAACCACGCAATTCTTCGAACAGCGGCTGGTTTCTACCCGGAGCTATTCCATTTCCCGTTCCAGCTCTGCAATGGCCGCTGTTCGAAATGTGTTCCTCTTCCTCACCATCTTCCTCGTCGGCGGTCCACCTCTTTCCTGTTCCCTCTCCGATAACGAGGCCCTCCTGAAGCTCAGGCAATCCTTCACCCATGCCGAATCCCTTAATTCCTGGGACCCGGACTCCCAACCCTGCGCCGCTCGATGGACTGGCATCATCTGCCACAGAGGCGTCATCACCGGTCTCCATCTCACCGGCCTCCGACTCTCCGGCCAGATCGACATCGAAGCTCTCCTCCAGCTTCGCAGCCTTCGAACCGTCAGCCTCGTCAACAACAGCTTCGCCGGGCCGATTCCCGAGTTCAACAAAATCGGCGTTCTCAAATCCCTCCTTCTCACAGGGAATGATTTCTCCGGGGCTATTCCTACCGATTTCTTCGCATCCCTCGCCTCTCTCAAGAAGGTTTGGCTCTCTTCCAACAATTTCTCCGGCGACATTCCGCACTCTCTGGCGAAACTCTCGTATCTAATCGAACTCCATCTCGAAAACAACCAATTCTCCGGCCAAATCCCTCACCTACACCAGGCCGCCTTAACCTCTCTAGATCTCTCCAATAACAAGCTGGAGGGAGAAATTCCAGAGAATCTAGCGAAGTTCGACGCGAACGCATTTGCCGGAAACGAAGGTCTGTGTGGGAAGCCGCTCCCGAAATCATGCGACGGCAATAACGCGCAGATTTCGGAGGAAAACGCGTCTTCTCCGGGGGAATCACAAGGCAGCGTCTCGAAATTAGTAGTGGCGTCGTTGATAGCGGTGGCTGTATTTCTGGTGGTGGTTATGTTTCTGAGCGCGTCGAAGCGGAGAGAGGAGAAGTTCAGCAAGCTAGGGCGGGAGCAGATGAATGAGATGGTGGAGGTGCACGTGCCGAGTTCCGGGCATAGGGAGCAGCTGAGTAGAAAAGGTGGGGATTCCAGGAGAGGGTCGCAACAGGGGAAGGGAGGGATGAGTGATCTTGTGGTAGTGAATGAAGAAAAGGGTACGTTTGGGATGGCGGATTTGATGAAGGCGGCGGCGGAGGTGCTGGGGAATGGTGGGTTGGGGTCGGCTTACAAGGCCGTCATGGCAAATGGGCTGTCGGTGGTGGTGAAAAGAATCAGAGAAATGAACAAATTAGGGAAGGATGGGTTCGATGCAGAGATGAGGCGCATGGGACGGCTGCAGCATCACAATATTCTCACGCCTTTGGCGTACCATTACAGGCGGGAGGAGAAGCTGCTGGTGTCCGAGTACATTCCTAAAGGCAGCCTCTTGTATGTGTTGCATGGGGACCGTGGAGCCTGCCATGCGGAGCTTAACTGGGGCACCCGGTTAAAGATCGTGAAGGGGATCGCCAGAGGCCTAGGGTTTCTCCATTCCGAGTTCGCGACCTACGATCTGCCGCATGGGAATCTGAAATCAAGCAACATTCTTCTAAGCGACACCTACGAGCCACTTCTCAGCGACTACGCGTTTCATCCGCTAATCAATCCAAACAACGCGACGGAGGCAATGTTTGCGTACAAATCCCCGGAGTACGCGCATCGGCAGGAAGTTTCTCCAAAATCTGATGTTTATTGCCTTGGAATTATCATTCTAGAAATTCTGACCGGCAAATTTCCTTCTCAATATCTTAGCAATGGCAAGGGAGGGACCGATGTGGTGCAGTGGGTCTCCTCCGCAGTAGCCGAGAAGAGGGAGGCGGAACTCATAGACCCTGAGATAGCAAACGACGCGAATGCAGTGAGTCGGATGGTCGAGCTCCTTAGAATCGGCGCCGATTGCACCCAATCCAATCCTCAGCAACGGCCAGAAATGAAGGAAGCCATTAGGAGAATAGANGAAATATGAGGAATCATAAATTTGTTTTTGGAAACTAAGAAAAGGGCATATTGAGGGGGGGGAGGGGGGAGGGGGGATGTGGGGAAGAACACAAAAAACTAATGTGGTAGATGGATAG

Coding sequence (CDS)

ATGGCCGCTGTTCGAAATGTGTTCCTCTTCCTCACCATCTTCCTCGTCGGCGGTCCACCTCTTTCCTGTTCCCTCTCCGATAACGAGGCCCTCCTGAAGCTCAGGCAATCCTTCACCCATGCCGAATCCCTTAATTCCTGGGACCCGGACTCCCAACCCTGCGCCGCTCGATGGACTGGCATCATCTGCCACAGAGGCGTCATCACCGGTCTCCATCTCACCGGCCTCCGACTCTCCGGCCAGATCGACATCGAAGCTCTCCTCCAGCTTCGCAGCCTTCGAACCGTCAGCCTCGTCAACAACAGCTTCGCCGGGCCGATTCCCGAGTTCAACAAAATCGGCGTTCTCAAATCCCTCCTTCTCACAGGGAATGATTTCTCCGGGGCTATTCCTACCGATTTCTTCGCATCCCTCGCCTCTCTCAAGAAGGTTTGGCTCTCTTCCAACAATTTCTCCGGCGACATTCCGCACTCTCTGGCGAAACTCTCGTATCTAATCGAACTCCATCTCGAAAACAACCAATTCTCCGGCCAAATCCCTCACCTACACCAGGCCGCCTTAACCTCTCTAGATCTCTCCAATAACAAGCTGGAGGGAGAAATTCCAGAGAATCTAGCGAAGTTCGACGCGAACGCATTTGCCGGAAACGAAGGTCTGTGTGGGAAGCCGCTCCCGAAATCATGCGACGGCAATAACGCGCAGATTTCGGAGGAAAACGCGTCTTCTCCGGGGGAATCACAAGGCAGCGTCTCGAAATTAGTAGTGGCGTCGTTGATAGCGGTGGCTGTATTTCTGGTGGTGGTTATGTTTCTGAGCGCGTCGAAGCGGAGAGAGGAGAAGTTCAGCAAGCTAGGGCGGGAGCAGATGAATGAGATGGTGGAGGTGCACGTGCCGAGTTCCGGGCATAGGGAGCAGCTGAGTAGAAAAGGTGGGGATTCCAGGAGAGGGTCGCAACAGGGGAAGGGAGGGATGAGTGATCTTGTGGTAGTGAATGAAGAAAAGGGTACGTTTGGGATGGCGGATTTGATGAAGGCGGCGGCGGAGGTGCTGGGGAATGGTGGGTTGGGGTCGGCTTACAAGGCCGTCATGGCAAATGGGCTGTCGGTGGTGGTGAAAAGAATCAGAGAAATGAACAAATTAGGGAAGGATGGGTTCGATGCAGAGATGAGGCGCATGGGACGGCTGCAGCATCACAATATTCTCACGCCTTTGGCGTACCATTACAGGCGGGAGGAGAAGCTGCTGGTGTCCGAGTACATTCCTAAAGGCAGCCTCTTGTATGTGTTGCATGGGGACCGTGGAGCCTGCCATGCGGAGCTTAACTGGGGCACCCGGTTAAAGATCGTGAAGGGGATCGCCAGAGGCCTAGGGTTTCTCCATTCCGAGTTCGCGACCTACGATCTGCCGCATGGGAATCTGAAATCAAGCAACATTCTTCTAAGCGACACCTACGAGCCACTTCTCAGCGACTACGCGTTTCATCCGCTAATCAATCCAAACAACGCGACGGAGGCAATGTTTGCGTACAAATCCCCGGAGTACGCGCATCGGCAGGAAGTTTCTCCAAAATCTGATGTTTATTGCCTTGGAATTATCATTCTAGAAATTCTGACCGGCAAATTTCCTTCTCAATATCTTAGCAATGGCAAGGGAGGGACCGATGTGGTGCAGTGGGTCTCCTCCGCAGTAGCCGAGAAGAGGGAGGCGGAACTCATAGACCCTGAGATAGCAAACGACGCGAATGCAGTGAGTCGGATGGTCGAGCTCCTTAGAATCGGCGCCGATTGCACCCAATCCAATCCTCAGCAACGGCCAGAAATGAAGGAAGCCATTAGGAGAATAGANGAAATATGA

Protein sequence

MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWDPDSQPCAARWTGIICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLLLTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIPHLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEENASSPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSSGHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYKAVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYIPKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILLSDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILTGKFPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSNPQQRPEMKEAIRRIXEI
Homology
BLAST of CmaCh12G013330 vs. ExPASy Swiss-Prot
Match: Q9M1L7 (Pollen receptor-like kinase 3 OS=Arabidopsis thaliana OX=3702 GN=PRK3 PE=1 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 4.0e-185
Identity = 336/633 (53.08%), Postives = 449/633 (70.93%), Query Frame = 0

Query: 7   VFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAE-SLNSWDPDSQPCAARWTGIICHR 66
           +FL   +      P   ++S++E L++ ++S    +  LNSW   + PC  +W GI C +
Sbjct: 5   LFLCFLLICFSFTPSLQNVSESEPLVRFKRSVNITKGDLNSWRTGTDPCNGKWFGIYCQK 64

Query: 67  G-VITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLLLTGN 126
           G  ++G+H+T L LSG I+IE L  L +LRT+ L NN  +GP+P F K+  LKSLLL+ N
Sbjct: 65  GQTVSGIHVTRLGLSGTINIEDLKDLPNLRTIRLDNNLLSGPLPPFFKLPGLKSLLLSNN 124

Query: 127 DFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIPHLHQ 186
            FSG I  DFF     LK+V+L +N  SG IP SL +L+ L ELH++ NQF+G+IP L  
Sbjct: 125 SFSGEIADDFFKETPQLKRVFLDNNRLSGKIPASLMQLAGLEELHMQGNQFTGEIPPLTD 184

Query: 187 A--ALTSLDLSNNKLEGEIPENLA--KFDANAFAGNEGLCGKPLPKSCDGNNAQISEENA 246
               L SLDLSNN LEGEIP  ++  K     F GN+ LCG PL   CD       + ++
Sbjct: 185 GNKVLKSLDLSNNDLEGEIPITISDRKNLEMKFEGNQRLCGSPLNIECD------EKPSS 244

Query: 247 SSPGESQGSVSKLVVASLIAVAVFLVVVMFLSA-SKRREEKFSKLGREQMN--EMVEVHV 306
           +  G  + + +K +   ++ + +FL VV  ++   K+R+ +F  LG++ ++  E VEV V
Sbjct: 245 TGSGNEKNNTAKAIFMVILFLLIFLFVVAIITRWKKKRQPEFRMLGKDHLSDQESVEVRV 304

Query: 307 PSS------GHREQLSRKGGDSRRGSQQGKG-------GMSDLVVVNEEKGTFGMADLMK 366
           P S        +++ + +G   +  S  GKG       GM D+++VN EKG+FG+ DLMK
Sbjct: 305 PDSIKKPIDSSKKRSNAEGSSKKGSSHNGKGAGGGPGSGMGDIIMVNSEKGSFGLPDLMK 364

Query: 367 AAAEVLGNGGLGSAYKAVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPL 426
           AAAEVLGNG LGSAYKAVMANGLSVVVKRIR+MNKL ++ FD EM+R G+L+H N+LTPL
Sbjct: 365 AAAEVLGNGSLGSAYKAVMANGLSVVVKRIRDMNKLAREAFDTEMQRFGKLRHPNVLTPL 424

Query: 427 AYHYRREEKLLVSEYIPKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFA 486
           AYHYRREEKL+VSEY+PK SLLYVLHGDRG  H+EL W TRLKI++G+ARG+ FLH EFA
Sbjct: 425 AYHYRREEKLVVSEYMPKSSLLYVLHGDRGVYHSELTWATRLKIIQGVARGMDFLHEEFA 484

Query: 487 TYDLPHGNLKSSNILLSDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKS 546
           +YDLPHGNLKSSN+LLS+TYEPL+SDYAF PL+ PNNA++A+FA+KSPE+   Q+VSPKS
Sbjct: 485 SYDLPHGNLKSSNVLLSETYEPLISDYAFLPLLQPNNASQALFAFKSPEFVQNQQVSPKS 544

Query: 547 DVYCLGIIILEILTGKFPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVS 606
           DVYCLGII+LE++TGKFPSQYL+ GKGGTD+V+WV S++A+ +E ELIDPEIA++ +++ 
Sbjct: 545 DVYCLGIIVLEVMTGKFPSQYLNTGKGGTDIVEWVQSSIAQHKEEELIDPEIASNTDSIK 604

Query: 607 RMVELLRIGADCTQSNPQQRPEMKEAIRRIXEI 618
           +MVELLRIGA C  SNP +R  MKE +RRI  +
Sbjct: 605 QMVELLRIGAACIASNPNERQNMKEIVRRIERV 631

BLAST of CmaCh12G013330 vs. ExPASy Swiss-Prot
Match: Q3E991 (Pollen receptor-like kinase 6 OS=Arabidopsis thaliana OX=3702 GN=PRK6 PE=1 SV=4)

HSP 1 Score: 612.5 bits (1578), Expect = 5.4e-174
Identity = 334/657 (50.84%), Postives = 444/657 (67.58%), Query Frame = 0

Query: 2   AAVRNVFLFLTIFLVG---GPPLSCSLSDNEALLKLRQSFTHAE-SLNSWDPDSQPCAAR 61
           AAV N   FL I L+     P L   +S++E L++ + S    +  LNSW   + PC+ +
Sbjct: 3   AAVLNPGFFLLILLLSFSISPSLQ-YVSESEPLVRFKNSVKITKGDLNSWREGTDPCSGK 62

Query: 62  WTGIICHRGV-ITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVL 121
           W GI C +G+ ++G+H+T L LSG I ++ L  L +L+T+ L NN  +GP+P F K+  L
Sbjct: 63  WFGIYCQKGLTVSGIHVTRLGLSGTITVDDLKDLPNLKTIRLDNNLLSGPLPHFFKLRGL 122

Query: 122 KSLLLTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFS 181
           KSL+L+ N FSG I  DFF  ++ LK+++L  N F G IP S+ +L  L ELH+++N  +
Sbjct: 123 KSLMLSNNSFSGEIRDDFFKDMSKLKRLFLDHNKFEGSIPSSITQLPQLEELHMQSNNLT 182

Query: 182 GQIPHLHQAA--LTSLDLSNNKLEGEIPENLA--KFDANAFAGNEGLCGKPLPKSCDGNN 241
           G+IP    +   L  LDLS N L+G +P+++A  K  A     NE LCG  +   C+   
Sbjct: 183 GEIPPEFGSMKNLKVLDLSTNSLDGIVPQSIADKKNLAVNLTENEYLCGPVVDVGCENIE 242

Query: 242 AQISEEN--ASSPGESQGSVSKLVVASLIAVAVFLVVVMFL--SASKRREEK----FSKL 301
               +E    S P  S    S     + I V++ L+++ F+     KRR +K    F  L
Sbjct: 243 LNDPQEGQPPSKPSSSVPETSNKAAINAIMVSISLLLLFFIIVGVIKRRNKKKNPDFRML 302

Query: 302 GREQMNEMVEVHVPSS----------------GHREQLSR--------KGGDSRRGSQQG 361
              + N++VEV +  S                GH +  S         KGG+   G   G
Sbjct: 303 ANNRENDVVEVRISESSSTTAKRSTDSSRKRGGHSDDGSTKKGVSNIGKGGNGGGGGALG 362

Query: 362 KGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYKAVMANGLSVVVKRIREMNKL 421
            GGM D+++VN +KG+FG+ DLMKAAAEVLGNG LGSAYKAVM  GLSVVVKRIR+MN+L
Sbjct: 363 -GGMGDIIMVNTDKGSFGLPDLMKAAAEVLGNGSLGSAYKAVMTTGLSVVVKRIRDMNQL 422

Query: 422 GKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYIPKGSLLYVLHGDRGACHAEL 481
            ++ FD EMRR G+L+H NILTPLAYHYRREEKL+VSEY+PK SLLYVLHGDRG  H+EL
Sbjct: 423 AREPFDVEMRRFGKLRHPNILTPLAYHYRREEKLVVSEYMPKSSLLYVLHGDRGIYHSEL 482

Query: 482 NWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILLSDTYEPLLSDYAFHPLINPN 541
            W TRLKI++G+A G+ FLH EFA+YDLPHGNLKSSN+LLS+TYEPL+SDYAF PL+ P+
Sbjct: 483 TWATRLKIIQGVAHGMKFLHEEFASYDLPHGNLKSSNVLLSETYEPLISDYAFLPLLQPS 542

Query: 542 NATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILTGKFPSQYLSNGKGGTDVVQWVS 601
           NA++A+FA+K+PE+A  Q+VS KSDVYCLGIIILEILTGKFPSQYL+NGKGGTD+VQWV 
Sbjct: 543 NASQALFAFKTPEFAQTQQVSHKSDVYCLGIIILEILTGKFPSQYLNNGKGGTDIVQWVQ 602

Query: 602 SAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSNPQQRPEMKEAIRRIXEI 618
           S+VAE++E ELIDPEI N+  ++ +MVELLR+GA C  SNP +R +M+EA+RRI ++
Sbjct: 603 SSVAEQKEEELIDPEIVNNTESMRQMVELLRVGAACIASNPDERLDMREAVRRIEQV 657

BLAST of CmaCh12G013330 vs. ExPASy Swiss-Prot
Match: Q9LJY0 (Pollen receptor-like kinase 4 OS=Arabidopsis thaliana OX=3702 GN=PRK4 PE=1 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.9e-126
Identity = 240/603 (39.80%), Postives = 368/603 (61.03%), Query Frame = 0

Query: 26  SDNEALLKLRQSFTHAESLNSWDPDSQPC---AARWTGIICHRGVITGLHLTGLRLSGQI 85
           SD + LL+ + +  +A  ++SWDP   PC   +  W G++C  G + GL L G+ L+G++
Sbjct: 51  SDADCLLRFKDTLVNASFISSWDPSISPCKRNSENWFGVLCVTGNVWGLQLEGMGLTGKL 110

Query: 86  DIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLLLTGNDFSGAIPTDFFASLASLK 145
           D+E L  +++LRT+S +NN F G +P     G LKSL L+ N F+G IP D F  +  LK
Sbjct: 111 DLEPLAAIKNLRTLSFMNNKFNGSMPSVKNFGALKSLYLSNNRFTGEIPADAFDGMHHLK 170

Query: 146 KVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIPHLHQAALTSLDLSNNKLEGEIP 205
           K+ L++N F G IP SLA L  L+EL L  NQF G+IP+  Q  L      NN LEG IP
Sbjct: 171 KLLLANNAFRGSIPSSLAYLPMLLELRLNGNQFHGEIPYFKQKDLKLASFENNDLEGPIP 230

Query: 206 ENLAKFDANAFAGNEGLCGKPL-PKSCDGNNAQISEENASSPGESQGSVSKLVVA-SLIA 265
           E+L+  D  +F+GN+ LCG PL P S D  +   S +  SSP E   + S  ++A  LI 
Sbjct: 231 ESLSNMDPVSFSGNKNLCGPPLSPCSSDSGS---SPDLPSSPTEKNKNQSFFIIAIVLIV 290

Query: 266 VAVFLVVVMFLSA--SKRREEKFSKLGREQMNEMVEVHVPSSGHREQLSRKGGDSRRGSQ 325
           + + L+++  +      RR +  S       +   + +   S  ++    K  DS     
Sbjct: 291 IGIILMIISLVVCILHTRRRKSLSAYPSAGQDRTEKYNYDQSTDKD----KAADSVTSYT 350

Query: 326 QGKGGMSD---LVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYKAVMANGLSVVVKRIR 385
             +G + D   L+ + ++   F + DL++A+AEVLG+G  GS+YK  + +G  +VVKR +
Sbjct: 351 SRRGAVPDQNKLLFLQDDIQRFDLQDLLRASAEVLGSGSFGSSYKTGINSGQMLVVKRYK 410

Query: 386 EMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYIPKGSLLYVLHGDRGA 445
            MN +G+D F   MRR+GRL+H N+L  +AY+YRREEKLL++E++P  SL   LH +   
Sbjct: 411 HMNNVGRDEFHEHMRRLGRLKHPNLLPIVAYYYRREEKLLIAEFMPNRSLASHLHANHSV 470

Query: 446 CHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILLSDTYEPLLSDYAFHP 505
               L+W TRLKI++G+A+GLG+L +E  T  +PHG+LKSSN++L +++EPLL+DYA  P
Sbjct: 471 DQPGLDWPTRLKIIQGVAKGLGYLFNELTTLTIPHGHLKSSNVVLDESFEPLLTDYALRP 530

Query: 506 LINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILTGKFPSQYLSNG-KGGTD 565
           ++N   +   M +YKSPEY+ +  ++ K+DV+CLG++ILE+LTG+FP  YLS G      
Sbjct: 531 VMNSEQSHNLMISYKSPEYSLKGHLTKKTDVWCLGVLILELLTGRFPENYLSQGYDANMS 590

Query: 566 VVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSNPQQRPEMKEAIRRI 618
           +V WVS+ V EK+  ++ D E+    N  + M+ LL+IG  C + + ++R EM++A+ +I
Sbjct: 591 LVTWVSNMVKEKKTGDVFDKEMTGKKNCKAEMLNLLKIGLSCCEEDEERRMEMRDAVEKI 646

BLAST of CmaCh12G013330 vs. ExPASy Swiss-Prot
Match: C0LGU0 (Pollen receptor-like kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PRK1 PE=1 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 1.2e-125
Identity = 257/630 (40.79%), Postives = 382/630 (60.63%), Query Frame = 0

Query: 3   AVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFT--HAESLNSWDPDSQPCAARWTG 62
           +V NV + L   L+     +  LSD+EA+LK ++S       +L SW+  S PC   W+G
Sbjct: 10  SVYNVMVPLVCLLLFFSTPTHGLSDSEAILKFKESLVVGQENALASWNAKSPPCT--WSG 69

Query: 63  IICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLL 122
           ++C+ G +  L +  L LSG IDIEAL  L SLRT+S +NN F GP P+F K+  LKSL 
Sbjct: 70  VLCNGGSVWRLQMENLELSGSIDIEALSGLTSLRTLSFMNNKFEGPFPDFKKLAALKSLY 129

Query: 123 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIP 182
           L+ N F G IP D F  +  LKKV L+ N F+G IP S+AKL  L+EL L+ NQF+G+IP
Sbjct: 130 LSNNQFGGDIPGDAFEGMGWLKKVHLAQNKFTGQIPSSVAKLPKLLELRLDGNQFTGEIP 189

Query: 183 HL-HQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEEN 242
              HQ  L  L+LSNN L G IPE+L+  D   F GN+GL GKPL   CD    +   ++
Sbjct: 190 EFEHQLHL--LNLSNNALTGPIPESLSMTDPKVFEGNKGLYGKPLETECDSPYIEHPPQS 249

Query: 243 ASSPGESQGSVSKLVVASLIAVAVFLVV--VMFL---SASKRREEKFSKLGREQMNEMVE 302
            + P  S  S   LV+ +++A    L++  V+FL   S   ++     + G   + +   
Sbjct: 250 EARPKSS--SRGPLVITAIVAALTILIILGVIFLLNRSYKNKKPRLAVETGPSSLQKKTG 309

Query: 303 VHVPSSGHREQLSRKGGDSRRGSQQGK-----GGMSD--LVVVNEEKGTFGMADLMKAAA 362
           +       R+   RK  D R+GS   K      G+ +  L  + E++  F + DL+KA+A
Sbjct: 310 IREADQSRRD---RKKADHRKGSGTTKRMGAAAGVENTKLSFLREDREKFDLQDLLKASA 369

Query: 363 EVLGNGGLGSAYKAVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYH 422
           E+LG+G  G++YKAV+++G  +VVKR ++MN  G+D F   M+R+GRL HHN+L+ +AY+
Sbjct: 370 EILGSGCFGASYKAVLSSGQMMVVKRFKQMNNAGRDEFQEHMKRLGRLMHHNLLSIVAYY 429

Query: 423 YRREEKLLVSEYIPKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYD 482
           YR+EEKLLV ++  +GSL   LH ++      L+W TRLKIVKG+A+GL +LH +  +  
Sbjct: 430 YRKEEKLLVCDFAERGSLAINLHSNQSLGKPSLDWPTRLKIVKGVAKGLFYLHQDLPSLM 489

Query: 483 LPHGNLKSSNILLSDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVY 542
            PHG+LKSSN+LL+ T+EPLL+DY   PLIN   A   M AY+SPEY   + ++ K+DV+
Sbjct: 490 APHGHLKSSNVLLTKTFEPLLTDYGLIPLINQEKAQMHMAAYRSPEYLQHRRITKKTDVW 549

Query: 543 CLGIIILEILTGKFPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMV 602
            LGI+ILEILTGKFP+ +  + +   D+  WV+S         L D  +   ++   +++
Sbjct: 550 GLGILILEILTGKFPANFSQSSE--EDLASWVNSGFHGVWAPSLFDKGMGKTSHCEGQIL 609

Query: 603 ELLRIGADCTQSNPQQRPEMKEAIRRIXEI 618
           +LL IG +C + + ++R ++ +A+ +I E+
Sbjct: 610 KLLTIGLNCCEPDVEKRLDIGQAVEKIEEL 628

BLAST of CmaCh12G013330 vs. ExPASy Swiss-Prot
Match: Q9LPT1 (Pollen receptor-like kinase 5 OS=Arabidopsis thaliana OX=3702 GN=PRK5 PE=1 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 3.0e-124
Identity = 249/630 (39.52%), Postives = 375/630 (59.52%), Query Frame = 0

Query: 9   LFLTIFLVGGPPLSCSL-----SDNEALLKLRQSFTHAESLNSWDPDSQPC---AARWTG 68
           +F+ IF+    P++ S      SD + LL+ + +  +     SWDP S PC    A W G
Sbjct: 24  IFIIIFISVLCPVAMSQVVVPDSDADCLLRFKDTLANGSEFRSWDPLSSPCQGNTANWFG 83

Query: 69  IICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLL 128
           ++C    + GL L G+ L+G+++++ L+ +++LRT+S +NN+F GP+P+  +   LKSL 
Sbjct: 84  VLC-SNYVWGLQLEGMGLTGKLNLDPLVPMKNLRTISFMNNNFNGPMPQVKRFTSLKSLY 143

Query: 129 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIP 188
           L+ N FSG IP D F  +  LKK+ L++N F G IP SLA L  L+EL L  NQF GQIP
Sbjct: 144 LSNNRFSGEIPADAFLGMPLLKKILLANNAFRGTIPSSLASLPMLLELRLNGNQFQGQIP 203

Query: 189 HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNN-------- 248
              Q  L      NN L+G IPE+L   D  +FAGN+GLC  PL   C  ++        
Sbjct: 204 SFQQKDLKLASFENNDLDGPIPESLRNMDPGSFAGNKGLCDAPL-SPCSSSSPGVPVVPV 263

Query: 249 AQISEENASSP-GESQGSVSKLVVASLIAVAVFLVVV--MFLSASKRREEKFSKLGREQM 308
           + +  ++ S P G+  GS   L +  LI + + LV++  +F     RR    S       
Sbjct: 264 SPVDPKSTSPPTGKKAGSFYTLAII-LIVIGIILVIIALVFCFVQSRRRNFLSAYPSSAG 323

Query: 309 NEMVEV--HVPSSGHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAA 368
            E +E   +  S+    + +     +RRGS    GG   L+ V ++   F + DL++A+A
Sbjct: 324 KERIESYNYHQSTNKNNKPAESVNHTRRGSMPDPGGR--LLFVRDDIQRFDLQDLLRASA 383

Query: 369 EVLGNGGLGSAYKAVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYH 428
           EVLG+G  G++YKA +++G ++VVKR + MN +G+D F   MRR+GRL H NIL  +AY+
Sbjct: 384 EVLGSGTFGASYKAAISSGQTLVVKRYKHMNNVGRDEFHEHMRRLGRLNHPNILPLVAYY 443

Query: 429 YRREEKLLVSEYIPKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYD 488
           YRREEKLLV+E++P  SL   LH +     A L+W TRLKI+KG+A+GL +L  E  T  
Sbjct: 444 YRREEKLLVTEFMPNSSLASHLHANNS---AGLDWITRLKIIKGVAKGLSYLFDELPTLT 503

Query: 489 LPHGNLKSSNILLSDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEY--AHRQEVSPKSD 548
           +PHG++KSSNI+L D++EPLL+DYA  P+++  +A   M AYKSPEY  +  Q ++ K+D
Sbjct: 504 IPHGHMKSSNIVLDDSFEPLLTDYALRPMMSSEHAHNFMTAYKSPEYRPSKGQIITKKTD 563

Query: 549 VYCLGIIILEILTGKFPSQYLSNG-KGGTDVVQWVSSAVAEKREAELIDPEIANDANAVS 608
           V+C G++ILE+LTG+FP  YL+ G      +V WV+  V EK+  ++ D E+    N  +
Sbjct: 564 VWCFGVLILEVLTGRFPENYLTQGYDSNMSLVTWVNDMVKEKKTGDVFDKEMKGKKNCKA 623

Query: 609 RMVELLRIGADCTQSNPQQRPEMKEAIRRI 615
            M+ LL+IG  C +   ++R +M+E +  +
Sbjct: 624 EMINLLKIGLRCCEEEEERRMDMREVVEMV 645

BLAST of CmaCh12G013330 vs. ExPASy TrEMBL
Match: A0A6J1HQR8 (pollen receptor-like kinase 3 OS=Cucurbita maxima OX=3661 GN=LOC111465780 PE=4 SV=1)

HSP 1 Score: 1213.7 bits (3139), Expect = 0.0e+00
Identity = 617/617 (100.00%), Postives = 617/617 (100.00%), Query Frame = 0

Query: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWDPDSQPCAARWTG 60
           MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWDPDSQPCAARWTG
Sbjct: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWDPDSQPCAARWTG 60

Query: 61  IICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLL 120
           IICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLL
Sbjct: 61  IICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLL 120

Query: 121 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIP 180
           LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIP
Sbjct: 121 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIP 180

Query: 181 HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEENA 240
           HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEENA
Sbjct: 181 HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEENA 240

Query: 241 SSPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS 300
           SSPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS
Sbjct: 241 SSPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS 300

Query: 301 GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK 360
           GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK
Sbjct: 301 GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK 360

Query: 361 AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI 420
           AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI
Sbjct: 361 AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI 420

Query: 421 PKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL 480
           PKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL
Sbjct: 421 PKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL 480

Query: 481 SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILTGK 540
           SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILTGK
Sbjct: 481 SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILTGK 540

Query: 541 FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN 600
           FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN
Sbjct: 541 FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN 600

Query: 601 PQQRPEMKEAIRRIXEI 618
           PQQRPEMKEAIRRIXEI
Sbjct: 601 PQQRPEMKEAIRRIXEI 617

BLAST of CmaCh12G013330 vs. ExPASy TrEMBL
Match: A0A6J1FAK5 (pollen receptor-like kinase 3 OS=Cucurbita moschata OX=3662 GN=LOC111443910 PE=4 SV=1)

HSP 1 Score: 1195.3 bits (3091), Expect = 0.0e+00
Identity = 607/617 (98.38%), Postives = 611/617 (99.03%), Query Frame = 0

Query: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWDPDSQPCAARWTG 60
           MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSW+ DSQPCAARWTG
Sbjct: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWNQDSQPCAARWTG 60

Query: 61  IICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLL 120
           IICHRGVITGLHLTGLRLSGQIDIEALL+LRSLRTVSLVNNSFAGPIPEFN+IGVLKSLL
Sbjct: 61  IICHRGVITGLHLTGLRLSGQIDIEALLELRSLRTVSLVNNSFAGPIPEFNRIGVLKSLL 120

Query: 121 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIP 180
           LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSG+IP
Sbjct: 121 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGKIP 180

Query: 181 HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEENA 240
           HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCD NNAQISEENA
Sbjct: 181 HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDSNNAQISEENA 240

Query: 241 SSPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS 300
           S PGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS
Sbjct: 241 SPPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS 300

Query: 301 GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK 360
           GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK
Sbjct: 301 GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK 360

Query: 361 AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI 420
           AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI
Sbjct: 361 AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI 420

Query: 421 PKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL 480
           PKGSLLYVLHGDRGACHAELNW TRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL
Sbjct: 421 PKGSLLYVLHGDRGACHAELNWATRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL 480

Query: 481 SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILTGK 540
           SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYA RQEVSPKSDVYCLGIIILEILTGK
Sbjct: 481 SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAQRQEVSPKSDVYCLGIIILEILTGK 540

Query: 541 FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN 600
           FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN
Sbjct: 541 FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN 600

Query: 601 PQQRPEMKEAIRRIXEI 618
           PQQRPEMKEAIRRI EI
Sbjct: 601 PQQRPEMKEAIRRIEEI 617

BLAST of CmaCh12G013330 vs. ExPASy TrEMBL
Match: A0A5D3CFY4 (Pollen receptor-like kinase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G001520 PE=4 SV=1)

HSP 1 Score: 1008.4 bits (2606), Expect = 1.2e-290
Identity = 507/619 (81.91%), Postives = 564/619 (91.11%), Query Frame = 0

Query: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWDPDSQPCAARWTG 60
           M AVR VFL + +   GG PL C LS+NEALLKL++SFTH+ESLNSW+PDS PC+A+WTG
Sbjct: 1   MGAVRIVFLIILVG-GGGLPLCCPLSENEALLKLKESFTHSESLNSWNPDSVPCSAQWTG 60

Query: 61  IICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLL 120
           IIC+RGVITGLHL+ L+LSG+ID+EALLQLR LRT+S VNN F+GPIPEFNKIGVLKSLL
Sbjct: 61  IICNRGVITGLHLSDLQLSGKIDVEALLQLRGLRTISFVNNKFSGPIPEFNKIGVLKSLL 120

Query: 121 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIP 180
           LTGN FSGAIP+DFF+SL SLKKVWLSSNNFSG+IPHSLA+LS+LIELHLE+NQFSG IP
Sbjct: 121 LTGNHFSGAIPSDFFSSLTSLKKVWLSSNNFSGNIPHSLAQLSHLIELHLESNQFSGPIP 180

Query: 181 HL-HQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEEN 240
           HL H + +TSLDLSNNKLEG+IP+ L+KFDA AFAGNEGLCG PLPKSC     +  +  
Sbjct: 181 HLKHASIITSLDLSNNKLEGQIPDILSKFDAKAFAGNEGLCGNPLPKSCGARIPEDQKPP 240

Query: 241 ASSPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPS 300
           +S PGESQG+VSKLVVASLIAV VFL+V +FLSASKRRE++FS LGREQM E+VEVHVPS
Sbjct: 241 SSPPGESQGNVSKLVVASLIAVTVFLMVFIFLSASKRREDEFSVLGREQMEEVVEVHVPS 300

Query: 301 SGHREQLSRK-GGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSA 360
           SGH +Q SRK GGDS+RGSQQGK GMSDLVVVNE+KG FG+ADLMKAAAEVLGNGGLGSA
Sbjct: 301 SGHDKQSSRKGGGDSKRGSQQGKAGMSDLVVVNEDKGIFGLADLMKAAAEVLGNGGLGSA 360

Query: 361 YKAVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSE 420
           YKAVM+NGLSVVVKR+REMNKLGKDGFDAEMRR+GRL+HHNILTPLAYHYRREEKLLVSE
Sbjct: 361 YKAVMSNGLSVVVKRMREMNKLGKDGFDAEMRRLGRLRHHNILTPLAYHYRREEKLLVSE 420

Query: 421 YIPKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNI 480
           YIPKGSLLYVLHGDRGACHA+LNW TRL+IV+GIARGLGFLHSEFATYDLPHGNLKSSN+
Sbjct: 421 YIPKGSLLYVLHGDRGACHADLNWATRLRIVQGIARGLGFLHSEFATYDLPHGNLKSSNV 480

Query: 481 LLSDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILT 540
           LL D YEPLLSDYAFHPLINPNNAT+AMFAY+SPEYA  QEVSPKSDVYCLGIIILEI+T
Sbjct: 481 LLCDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYAQYQEVSPKSDVYCLGIIILEIMT 540

Query: 541 GKFPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQ 600
            KFPSQYL+NGKGGTDVVQWVSSAV+EKREAELIDPEIAND +A+ RMV LL IGADCT 
Sbjct: 541 SKFPSQYLTNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTDALDRMVNLLTIGADCTH 600

Query: 601 SNPQQRPEMKEAIRRIXEI 618
           +NPQQRPEM+EAIRRI EI
Sbjct: 601 NNPQQRPEMREAIRRIEEI 618

BLAST of CmaCh12G013330 vs. ExPASy TrEMBL
Match: A0A1S3CB95 (pollen receptor-like kinase 3 OS=Cucumis melo OX=3656 GN=LOC103498999 PE=4 SV=1)

HSP 1 Score: 1008.1 bits (2605), Expect = 1.6e-290
Identity = 506/619 (81.74%), Postives = 565/619 (91.28%), Query Frame = 0

Query: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWDPDSQPCAARWTG 60
           M AVR VFL + +   GG PL C LS+NEALLKL++SFTH+ESLNSW+PDS PC+A+WTG
Sbjct: 53  MGAVRIVFLIILVG-GGGLPLCCPLSENEALLKLKESFTHSESLNSWNPDSVPCSAQWTG 112

Query: 61  IICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLL 120
           IIC+RGVITGLHL+ L+LSG+ID+EALLQLR LRT+S VNN F+GPIPEFNKIGVLKSLL
Sbjct: 113 IICNRGVITGLHLSDLQLSGKIDVEALLQLRGLRTISFVNNKFSGPIPEFNKIGVLKSLL 172

Query: 121 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIP 180
           LTGN FSGAIP+DFF+SL SLKKVWLSSNNFSG+IPHSLA+LS+LIELHLE+NQFSG IP
Sbjct: 173 LTGNHFSGAIPSDFFSSLTSLKKVWLSSNNFSGNIPHSLAQLSHLIELHLESNQFSGPIP 232

Query: 181 HL-HQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEEN 240
           HL H + +TSLDLSNNKLEG+IP+ L+KFDA AFAGNEGLCG PLPKSC     +  + +
Sbjct: 233 HLKHASIITSLDLSNNKLEGQIPDILSKFDAKAFAGNEGLCGNPLPKSCGARIPEDQKPS 292

Query: 241 ASSPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPS 300
           +S PGESQG+VSKLVVASLIAV VFL+V +FLSASKRRE++FS LGRE+M E+VEVHVPS
Sbjct: 293 SSPPGESQGNVSKLVVASLIAVTVFLMVFIFLSASKRREDEFSVLGRERMEEVVEVHVPS 352

Query: 301 SGHREQLSRK-GGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSA 360
           SGH +Q SRK GGDS+RGSQQGK GMSDLVVVNE+KG FG+ADLMKAAAEVLGNGGLGSA
Sbjct: 353 SGHDKQSSRKGGGDSKRGSQQGKAGMSDLVVVNEDKGIFGLADLMKAAAEVLGNGGLGSA 412

Query: 361 YKAVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSE 420
           YKAVM+NGLSVVVKR+REMNKLGKDGFDAEMRR+GRL+HHNILTPLAYHYRREEKLLVSE
Sbjct: 413 YKAVMSNGLSVVVKRMREMNKLGKDGFDAEMRRLGRLRHHNILTPLAYHYRREEKLLVSE 472

Query: 421 YIPKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNI 480
           YIPKGSLLYVLHGDRGACHA+LNW TRL+IV+GIARGLGFLHSEFATYDLPHGNLKSSN+
Sbjct: 473 YIPKGSLLYVLHGDRGACHADLNWATRLRIVQGIARGLGFLHSEFATYDLPHGNLKSSNV 532

Query: 481 LLSDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILT 540
           LL D YEPLLSDYAFHPLINPNNAT+AMFAY+SPEYA  QEVSPKSDVYCLGIIILEI+T
Sbjct: 533 LLCDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYAQYQEVSPKSDVYCLGIIILEIMT 592

Query: 541 GKFPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQ 600
            KFPSQYL+NGKGGTDVVQWVSSAV+EKREAELIDPEIAND +A+ RMV LL IGADCT 
Sbjct: 593 SKFPSQYLTNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTDALDRMVNLLTIGADCTH 652

Query: 601 SNPQQRPEMKEAIRRIXEI 618
           +NPQQRPEM+EAIRRI EI
Sbjct: 653 NNPQQRPEMREAIRRIEEI 670

BLAST of CmaCh12G013330 vs. ExPASy TrEMBL
Match: A0A0A0LIJ2 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000010 PE=4 SV=1)

HSP 1 Score: 1003.8 bits (2594), Expect = 3.1e-289
Identity = 507/622 (81.51%), Postives = 567/622 (91.16%), Query Frame = 0

Query: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWDPDSQPCAARWTG 60
           M AVR VFL + +   GG P  C LS+NEALLKL++SFTH+ESLNSW+PDS PC+ARW G
Sbjct: 1   MGAVRIVFLIILVG-GGGLPSCCPLSENEALLKLKESFTHSESLNSWNPDSVPCSARWIG 60

Query: 61  IICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLL 120
           IIC+RGVITGLHL+GL+LSG+ID+EALLQLR LRT+S V+N F+GPIPEFNKIGVLKSLL
Sbjct: 61  IICNRGVITGLHLSGLQLSGKIDVEALLQLRGLRTISFVDNQFSGPIPEFNKIGVLKSLL 120

Query: 121 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIP 180
           LTGN FSGAIP+DFF+SL SLKKVWLSSNNFSG+IPHSLA+LS+LIELHLE+NQFSG IP
Sbjct: 121 LTGNHFSGAIPSDFFSSLTSLKKVWLSSNNFSGNIPHSLAQLSHLIELHLESNQFSGPIP 180

Query: 181 HL-HQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEEN 240
           HL H + +TSL++SNNKLEG+IP+ L+KFDA AFAGNEGLCG PLPKSC    AQISE+ 
Sbjct: 181 HLKHASIITSLNVSNNKLEGQIPDILSKFDAKAFAGNEGLCGNPLPKSC---GAQISEDQ 240

Query: 241 ---ASSPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVH 300
              +S PGESQG++SKLVVASLIAV VFL+V +FLSASKRRE++FS LGREQM E+VEVH
Sbjct: 241 KPPSSPPGESQGNISKLVVASLIAVTVFLMVFIFLSASKRREDEFSVLGREQMEEVVEVH 300

Query: 301 VPSSGHREQLSRK-GGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGL 360
           VPSSGH +Q SR+ GGDS+RGSQQGK GMSDLVVVNE+KG FG+ADLMKAAAEVLGNGGL
Sbjct: 301 VPSSGHDKQSSRRGGGDSKRGSQQGKAGMSDLVVVNEDKGIFGLADLMKAAAEVLGNGGL 360

Query: 361 GSAYKAVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLL 420
           GSAYKAVM+NGLSVVVKR+REMNKLGKDGFDAEMRR+GRL+HHNILTPLAYHYRREEKLL
Sbjct: 361 GSAYKAVMSNGLSVVVKRMREMNKLGKDGFDAEMRRLGRLRHHNILTPLAYHYRREEKLL 420

Query: 421 VSEYIPKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKS 480
           VSEYIPKGSLLYVLHGDRGACHA+LNW TRL+IV+GIARGLGFLHSEFATYDLPHGNLKS
Sbjct: 421 VSEYIPKGSLLYVLHGDRGACHADLNWATRLRIVQGIARGLGFLHSEFATYDLPHGNLKS 480

Query: 481 SNILLSDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILE 540
           SN+LL D YEPLLSDYAFHPLINPNNAT+AMFAY+SPEYA  QEVSPKSDVYCLGIIILE
Sbjct: 481 SNVLLCDNYEPLLSDYAFHPLINPNNATQAMFAYRSPEYAQYQEVSPKSDVYCLGIIILE 540

Query: 541 ILTGKFPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGAD 600
           I+T KFPSQYL+NGKGGTDVVQWVSSAV+EKREAELIDPEIAND +A+ RMV LL IGAD
Sbjct: 541 IMTSKFPSQYLTNGKGGTDVVQWVSSAVSEKREAELIDPEIANDTDALDRMVHLLTIGAD 600

Query: 601 CTQSNPQQRPEMKEAIRRIXEI 618
           CT +NPQQRPEM+EAIRRI EI
Sbjct: 601 CTHNNPQQRPEMREAIRRIEEI 618

BLAST of CmaCh12G013330 vs. NCBI nr
Match: XP_022966058.1 (pollen receptor-like kinase 3 [Cucurbita maxima])

HSP 1 Score: 1213.7 bits (3139), Expect = 0.0e+00
Identity = 617/617 (100.00%), Postives = 617/617 (100.00%), Query Frame = 0

Query: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWDPDSQPCAARWTG 60
           MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWDPDSQPCAARWTG
Sbjct: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWDPDSQPCAARWTG 60

Query: 61  IICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLL 120
           IICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLL
Sbjct: 61  IICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLL 120

Query: 121 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIP 180
           LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIP
Sbjct: 121 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIP 180

Query: 181 HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEENA 240
           HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEENA
Sbjct: 181 HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEENA 240

Query: 241 SSPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS 300
           SSPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS
Sbjct: 241 SSPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS 300

Query: 301 GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK 360
           GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK
Sbjct: 301 GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK 360

Query: 361 AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI 420
           AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI
Sbjct: 361 AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI 420

Query: 421 PKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL 480
           PKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL
Sbjct: 421 PKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL 480

Query: 481 SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILTGK 540
           SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILTGK
Sbjct: 481 SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILTGK 540

Query: 541 FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN 600
           FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN
Sbjct: 541 FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN 600

Query: 601 PQQRPEMKEAIRRIXEI 618
           PQQRPEMKEAIRRIXEI
Sbjct: 601 PQQRPEMKEAIRRIXEI 617

BLAST of CmaCh12G013330 vs. NCBI nr
Match: KAG6586502.1 (Pollen receptor-like kinase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1196.8 bits (3095), Expect = 0.0e+00
Identity = 607/617 (98.38%), Postives = 612/617 (99.19%), Query Frame = 0

Query: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWDPDSQPCAARWTG 60
           MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSW+ DSQPCAARWTG
Sbjct: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWNQDSQPCAARWTG 60

Query: 61  IICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLL 120
           IICHRGVITGLHLTGLRLSGQIDIEALL+LRSLRTVSLVNNSFAGPIPEFN+IGVLKSLL
Sbjct: 61  IICHRGVITGLHLTGLRLSGQIDIEALLELRSLRTVSLVNNSFAGPIPEFNRIGVLKSLL 120

Query: 121 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIP 180
           LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSG+IP
Sbjct: 121 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGKIP 180

Query: 181 HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEENA 240
           HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCD NNAQISEENA
Sbjct: 181 HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDSNNAQISEENA 240

Query: 241 SSPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS 300
           SSPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS
Sbjct: 241 SSPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS 300

Query: 301 GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK 360
           GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK
Sbjct: 301 GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK 360

Query: 361 AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI 420
           AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI
Sbjct: 361 AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI 420

Query: 421 PKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL 480
           PKGSLLYVLHGDRGACHAELNW TRLKIVKG+ARGLGFLHSEFATYDLPHGNLKSSNILL
Sbjct: 421 PKGSLLYVLHGDRGACHAELNWATRLKIVKGVARGLGFLHSEFATYDLPHGNLKSSNILL 480

Query: 481 SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILTGK 540
           SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYA RQEVSPKSDVYCLGIIILEILTGK
Sbjct: 481 SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAQRQEVSPKSDVYCLGIIILEILTGK 540

Query: 541 FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN 600
           FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN
Sbjct: 541 FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN 600

Query: 601 PQQRPEMKEAIRRIXEI 618
           PQQRPEMKEAIRRI EI
Sbjct: 601 PQQRPEMKEAIRRIEEI 617

BLAST of CmaCh12G013330 vs. NCBI nr
Match: XP_022937521.1 (pollen receptor-like kinase 3 [Cucurbita moschata])

HSP 1 Score: 1195.3 bits (3091), Expect = 0.0e+00
Identity = 607/617 (98.38%), Postives = 611/617 (99.03%), Query Frame = 0

Query: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWDPDSQPCAARWTG 60
           MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSW+ DSQPCAARWTG
Sbjct: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWNQDSQPCAARWTG 60

Query: 61  IICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLL 120
           IICHRGVITGLHLTGLRLSGQIDIEALL+LRSLRTVSLVNNSFAGPIPEFN+IGVLKSLL
Sbjct: 61  IICHRGVITGLHLTGLRLSGQIDIEALLELRSLRTVSLVNNSFAGPIPEFNRIGVLKSLL 120

Query: 121 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIP 180
           LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSG+IP
Sbjct: 121 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGKIP 180

Query: 181 HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEENA 240
           HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCD NNAQISEENA
Sbjct: 181 HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDSNNAQISEENA 240

Query: 241 SSPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS 300
           S PGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS
Sbjct: 241 SPPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS 300

Query: 301 GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK 360
           GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK
Sbjct: 301 GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK 360

Query: 361 AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI 420
           AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI
Sbjct: 361 AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI 420

Query: 421 PKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL 480
           PKGSLLYVLHGDRGACHAELNW TRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL
Sbjct: 421 PKGSLLYVLHGDRGACHAELNWATRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL 480

Query: 481 SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILTGK 540
           SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYA RQEVSPKSDVYCLGIIILEILTGK
Sbjct: 481 SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAQRQEVSPKSDVYCLGIIILEILTGK 540

Query: 541 FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN 600
           FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN
Sbjct: 541 FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN 600

Query: 601 PQQRPEMKEAIRRIXEI 618
           PQQRPEMKEAIRRI EI
Sbjct: 601 PQQRPEMKEAIRRIEEI 617

BLAST of CmaCh12G013330 vs. NCBI nr
Match: XP_023537808.1 (pollen receptor-like kinase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1194.1 bits (3088), Expect = 0.0e+00
Identity = 605/617 (98.06%), Postives = 610/617 (98.87%), Query Frame = 0

Query: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWDPDSQPCAARWTG 60
           MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSW+ DSQPCAARWTG
Sbjct: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWNQDSQPCAARWTG 60

Query: 61  IICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLL 120
           IICHRGVITGLHLTGLRLSGQIDIEALL+LRSLRTVSLVNNSFAGPIPEFN+IGVLKSLL
Sbjct: 61  IICHRGVITGLHLTGLRLSGQIDIEALLELRSLRTVSLVNNSFAGPIPEFNRIGVLKSLL 120

Query: 121 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIP 180
           LTGNDFSG IPTDFF+SL SLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSG+IP
Sbjct: 121 LTGNDFSGVIPTDFFSSLTSLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGKIP 180

Query: 181 HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEENA 240
           HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEENA
Sbjct: 181 HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEENA 240

Query: 241 SSPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS 300
           S PGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS
Sbjct: 241 SPPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS 300

Query: 301 GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK 360
           GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK
Sbjct: 301 GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK 360

Query: 361 AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI 420
           AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI
Sbjct: 361 AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI 420

Query: 421 PKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL 480
           PKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL
Sbjct: 421 PKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL 480

Query: 481 SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILTGK 540
           SDTYEPLLSDYAFHPLINPNNATEAMF YKSPEYA RQEVSPKSDVYCLGIIILEILTGK
Sbjct: 481 SDTYEPLLSDYAFHPLINPNNATEAMFGYKSPEYAQRQEVSPKSDVYCLGIIILEILTGK 540

Query: 541 FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN 600
           FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN
Sbjct: 541 FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN 600

Query: 601 PQQRPEMKEAIRRIXEI 618
           PQQRPEMKEAIRRI EI
Sbjct: 601 PQQRPEMKEAIRRIEEI 617

BLAST of CmaCh12G013330 vs. NCBI nr
Match: KAG7021359.1 (Pollen receptor-like kinase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1177.9 bits (3046), Expect = 0.0e+00
Identity = 602/617 (97.57%), Postives = 606/617 (98.22%), Query Frame = 0

Query: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWDPDSQPCAARWTG 60
           MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSW+ DSQPCAARWTG
Sbjct: 1   MAAVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAESLNSWNQDSQPCAARWTG 60

Query: 61  IICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLL 120
           IICHRGVITGLHLTGLRLSGQIDIEALL+LRSLRTVSLVNNSFAGPIPEFN+IGVLKSLL
Sbjct: 61  IICHRGVITGLHLTGLRLSGQIDIEALLELRSLRTVSLVNNSFAGPIPEFNRIGVLKSLL 120

Query: 121 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIP 180
           LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSG+IP
Sbjct: 121 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGKIP 180

Query: 181 HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEENA 240
           HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCD NNAQISEENA
Sbjct: 181 HLHQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDSNNAQISEENA 240

Query: 241 SSPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS 300
           S PGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS
Sbjct: 241 SPPGESQGSVSKLVVASLIAVAVFLVVVMFLSASKRREEKFSKLGREQMNEMVEVHVPSS 300

Query: 301 GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK 360
           GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK
Sbjct: 301 GHREQLSRKGGDSRRGSQQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYK 360

Query: 361 AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI 420
           AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI
Sbjct: 361 AVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYI 420

Query: 421 PKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL 480
           PKGSLL     DRGACHAELNW TRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL
Sbjct: 421 PKGSLL-----DRGACHAELNWATRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILL 480

Query: 481 SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILTGK 540
           SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYA RQEVSPKSDVYCLGIIILEILTGK
Sbjct: 481 SDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAQRQEVSPKSDVYCLGIIILEILTGK 540

Query: 541 FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN 600
           FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN
Sbjct: 541 FPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSN 600

Query: 601 PQQRPEMKEAIRRIXEI 618
           PQQRPEMKEAIRRI EI
Sbjct: 601 PQQRPEMKEAIRRIEEI 612

BLAST of CmaCh12G013330 vs. TAIR 10
Match: AT3G42880.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 649.4 bits (1674), Expect = 2.8e-186
Identity = 336/633 (53.08%), Postives = 449/633 (70.93%), Query Frame = 0

Query: 7   VFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFTHAE-SLNSWDPDSQPCAARWTGIICHR 66
           +FL   +      P   ++S++E L++ ++S    +  LNSW   + PC  +W GI C +
Sbjct: 5   LFLCFLLICFSFTPSLQNVSESEPLVRFKRSVNITKGDLNSWRTGTDPCNGKWFGIYCQK 64

Query: 67  G-VITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLLLTGN 126
           G  ++G+H+T L LSG I+IE L  L +LRT+ L NN  +GP+P F K+  LKSLLL+ N
Sbjct: 65  GQTVSGIHVTRLGLSGTINIEDLKDLPNLRTIRLDNNLLSGPLPPFFKLPGLKSLLLSNN 124

Query: 127 DFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIPHLHQ 186
            FSG I  DFF     LK+V+L +N  SG IP SL +L+ L ELH++ NQF+G+IP L  
Sbjct: 125 SFSGEIADDFFKETPQLKRVFLDNNRLSGKIPASLMQLAGLEELHMQGNQFTGEIPPLTD 184

Query: 187 A--ALTSLDLSNNKLEGEIPENLA--KFDANAFAGNEGLCGKPLPKSCDGNNAQISEENA 246
               L SLDLSNN LEGEIP  ++  K     F GN+ LCG PL   CD       + ++
Sbjct: 185 GNKVLKSLDLSNNDLEGEIPITISDRKNLEMKFEGNQRLCGSPLNIECD------EKPSS 244

Query: 247 SSPGESQGSVSKLVVASLIAVAVFLVVVMFLSA-SKRREEKFSKLGREQMN--EMVEVHV 306
           +  G  + + +K +   ++ + +FL VV  ++   K+R+ +F  LG++ ++  E VEV V
Sbjct: 245 TGSGNEKNNTAKAIFMVILFLLIFLFVVAIITRWKKKRQPEFRMLGKDHLSDQESVEVRV 304

Query: 307 PSS------GHREQLSRKGGDSRRGSQQGKG-------GMSDLVVVNEEKGTFGMADLMK 366
           P S        +++ + +G   +  S  GKG       GM D+++VN EKG+FG+ DLMK
Sbjct: 305 PDSIKKPIDSSKKRSNAEGSSKKGSSHNGKGAGGGPGSGMGDIIMVNSEKGSFGLPDLMK 364

Query: 367 AAAEVLGNGGLGSAYKAVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPL 426
           AAAEVLGNG LGSAYKAVMANGLSVVVKRIR+MNKL ++ FD EM+R G+L+H N+LTPL
Sbjct: 365 AAAEVLGNGSLGSAYKAVMANGLSVVVKRIRDMNKLAREAFDTEMQRFGKLRHPNVLTPL 424

Query: 427 AYHYRREEKLLVSEYIPKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFA 486
           AYHYRREEKL+VSEY+PK SLLYVLHGDRG  H+EL W TRLKI++G+ARG+ FLH EFA
Sbjct: 425 AYHYRREEKLVVSEYMPKSSLLYVLHGDRGVYHSELTWATRLKIIQGVARGMDFLHEEFA 484

Query: 487 TYDLPHGNLKSSNILLSDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKS 546
           +YDLPHGNLKSSN+LLS+TYEPL+SDYAF PL+ PNNA++A+FA+KSPE+   Q+VSPKS
Sbjct: 485 SYDLPHGNLKSSNVLLSETYEPLISDYAFLPLLQPNNASQALFAFKSPEFVQNQQVSPKS 544

Query: 547 DVYCLGIIILEILTGKFPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVS 606
           DVYCLGII+LE++TGKFPSQYL+ GKGGTD+V+WV S++A+ +E ELIDPEIA++ +++ 
Sbjct: 545 DVYCLGIIVLEVMTGKFPSQYLNTGKGGTDIVEWVQSSIAQHKEEELIDPEIASNTDSIK 604

Query: 607 RMVELLRIGADCTQSNPQQRPEMKEAIRRIXEI 618
           +MVELLRIGA C  SNP +R  MKE +RRI  +
Sbjct: 605 QMVELLRIGAACIASNPNERQNMKEIVRRIERV 631

BLAST of CmaCh12G013330 vs. TAIR 10
Match: AT5G20690.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 612.5 bits (1578), Expect = 3.8e-175
Identity = 334/657 (50.84%), Postives = 444/657 (67.58%), Query Frame = 0

Query: 2   AAVRNVFLFLTIFLVG---GPPLSCSLSDNEALLKLRQSFTHAE-SLNSWDPDSQPCAAR 61
           AAV N   FL I L+     P L   +S++E L++ + S    +  LNSW   + PC+ +
Sbjct: 3   AAVLNPGFFLLILLLSFSISPSLQ-YVSESEPLVRFKNSVKITKGDLNSWREGTDPCSGK 62

Query: 62  WTGIICHRGV-ITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVL 121
           W GI C +G+ ++G+H+T L LSG I ++ L  L +L+T+ L NN  +GP+P F K+  L
Sbjct: 63  WFGIYCQKGLTVSGIHVTRLGLSGTITVDDLKDLPNLKTIRLDNNLLSGPLPHFFKLRGL 122

Query: 122 KSLLLTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFS 181
           KSL+L+ N FSG I  DFF  ++ LK+++L  N F G IP S+ +L  L ELH+++N  +
Sbjct: 123 KSLMLSNNSFSGEIRDDFFKDMSKLKRLFLDHNKFEGSIPSSITQLPQLEELHMQSNNLT 182

Query: 182 GQIPHLHQAA--LTSLDLSNNKLEGEIPENLA--KFDANAFAGNEGLCGKPLPKSCDGNN 241
           G+IP    +   L  LDLS N L+G +P+++A  K  A     NE LCG  +   C+   
Sbjct: 183 GEIPPEFGSMKNLKVLDLSTNSLDGIVPQSIADKKNLAVNLTENEYLCGPVVDVGCENIE 242

Query: 242 AQISEEN--ASSPGESQGSVSKLVVASLIAVAVFLVVVMFL--SASKRREEK----FSKL 301
               +E    S P  S    S     + I V++ L+++ F+     KRR +K    F  L
Sbjct: 243 LNDPQEGQPPSKPSSSVPETSNKAAINAIMVSISLLLLFFIIVGVIKRRNKKKNPDFRML 302

Query: 302 GREQMNEMVEVHVPSS----------------GHREQLSR--------KGGDSRRGSQQG 361
              + N++VEV +  S                GH +  S         KGG+   G   G
Sbjct: 303 ANNRENDVVEVRISESSSTTAKRSTDSSRKRGGHSDDGSTKKGVSNIGKGGNGGGGGALG 362

Query: 362 KGGMSDLVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYKAVMANGLSVVVKRIREMNKL 421
            GGM D+++VN +KG+FG+ DLMKAAAEVLGNG LGSAYKAVM  GLSVVVKRIR+MN+L
Sbjct: 363 -GGMGDIIMVNTDKGSFGLPDLMKAAAEVLGNGSLGSAYKAVMTTGLSVVVKRIRDMNQL 422

Query: 422 GKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYIPKGSLLYVLHGDRGACHAEL 481
            ++ FD EMRR G+L+H NILTPLAYHYRREEKL+VSEY+PK SLLYVLHGDRG  H+EL
Sbjct: 423 AREPFDVEMRRFGKLRHPNILTPLAYHYRREEKLVVSEYMPKSSLLYVLHGDRGIYHSEL 482

Query: 482 NWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILLSDTYEPLLSDYAFHPLINPN 541
            W TRLKI++G+A G+ FLH EFA+YDLPHGNLKSSN+LLS+TYEPL+SDYAF PL+ P+
Sbjct: 483 TWATRLKIIQGVAHGMKFLHEEFASYDLPHGNLKSSNVLLSETYEPLISDYAFLPLLQPS 542

Query: 542 NATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILTGKFPSQYLSNGKGGTDVVQWVS 601
           NA++A+FA+K+PE+A  Q+VS KSDVYCLGIIILEILTGKFPSQYL+NGKGGTD+VQWV 
Sbjct: 543 NASQALFAFKTPEFAQTQQVSHKSDVYCLGIIILEILTGKFPSQYLNNGKGGTDIVQWVQ 602

Query: 602 SAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSNPQQRPEMKEAIRRIXEI 618
           S+VAE++E ELIDPEI N+  ++ +MVELLR+GA C  SNP +R +M+EA+RRI ++
Sbjct: 603 SSVAEQKEEELIDPEIVNNTESMRQMVELLRVGAACIASNPDERLDMREAVRRIEQV 657

BLAST of CmaCh12G013330 vs. TAIR 10
Match: AT1G72460.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 521.9 bits (1343), Expect = 6.8e-148
Identity = 288/613 (46.98%), Postives = 404/613 (65.91%), Query Frame = 0

Query: 24  SLSDNEALLKLRQSFTHAESLNSWDPDSQPCAA--RWTGIICHRGVITGLHLTGLRLSGQ 83
           S++++E+LLK ++S  + +SL+SW P+S+PC A  RW G++C++  + GL +  + LSG+
Sbjct: 21  SITESESLLKFKKSLNNTKSLDSWTPESEPCGASQRWIGLLCNKNSVFGLQIEQMGLSGK 80

Query: 84  IDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLLLTGNDFSGAIPTDFFASLASL 143
           +D+  L  L SLRT+S++NNSF+G IPEFN++  LKSL ++GN FSG IP+D+F ++ SL
Sbjct: 81  VDVAPLKDLPSLRTISIMNNSFSGDIPEFNRLTALKSLYISGNRFSGNIPSDYFETMVSL 140

Query: 144 KKVWLSSNNFSGDIPHSLA-KLSYLIELHLENNQFSGQIPHLHQAALTSLDLSNNKLEGE 203
           KK WLS+N+FSG IP SLA  L  LIEL LENNQF G IP+  Q  L  +DLSNN+L GE
Sbjct: 141 KKAWLSNNHFSGLIPISLATTLPNLIELRLENNQFIGSIPNFTQTTLAIVDLSNNQLTGE 200

Query: 204 IPENLAKFDANAFAGNEGLCGKPLPKSC-DGNNAQISEENASSPGESQGSVSKLVVASL- 263
           IP  L KFDA  FAGN GLCG  L   C    N+  S     +  ++  S   L  ++L 
Sbjct: 201 IPPGLLKFDAKTFAGNSGLCGAKLSTPCPQPKNSTASITIEGTMKDANKSKYFLAFSTLG 260

Query: 264 IAVAVFLVVVMFLSASKRREEKFSKLGREQMN---EMVEVHVPSSGHREQLSRKGGDSRR 323
           + + V LV + F    K+R  K ++   EQ N   + ++V V  S      SR+   SR 
Sbjct: 261 VLLIVVLVSLAFRKKKKKRRRKKARRTSEQDNSDDQQIQVTVEGSNS----SRQSRSSRS 320

Query: 324 GS-QQGKGGMSDLVVVNEEKGTFGMADLMKAAAEVLGN-----------GGLGSAYKAVM 383
           G   +G  G SDLV+VN+EKG F ++DLMKAAA VLGN           GG+GSAYKAV+
Sbjct: 321 GELNKGVAGTSDLVMVNKEKGVFRLSDLMKAAAHVLGNPGGGSNRPRSSGGVGSAYKAVL 380

Query: 384 ANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYIPKG 443
           +NG++VVVKR+  MN++  D FD E+R++G LQH N+LTPLAYH+R++EKLLV E++P  
Sbjct: 381 SNGVTVVVKRVTVMNQVSVDVFDKEIRKLGSLQHKNVLTPLAYHFRQDEKLLVFEFVPNL 440

Query: 444 SLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILLSDT 503
           +LL+ LHGD      +L+W +RLKI++GIARG+ +LH E    +LPHGNLKSSNI L++ 
Sbjct: 441 NLLHRLHGDHE--EFQLDWPSRLKIIQGIARGMWYLHRELGFLNLPHGNLKSSNIFLAED 500

Query: 504 YEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILTGKFPS 563
            EPL+S++    LINP+  ++++ A+KSPE      VS KSDV+  G+++LEILTGKFPS
Sbjct: 501 GEPLISEFGLQKLINPDAQSQSLVAFKSPEADRDGTVSAKSDVFSFGVVVLEILTGKFPS 560

Query: 564 QYLS-NGKGGTDVVQWVSSAVAEKREAELIDPEI----ANDANAVSRMVELLRIGADCTQ 612
           QY   N  GG ++V+W+ SA+ +    +L+ P +    A D      +  +LRIG  CT+
Sbjct: 561 QYAGLNRAGGANLVEWLGSALEQGGWMDLLHPMVVTAAAEDKIMEEEIENVLRIGVRCTR 620

BLAST of CmaCh12G013330 vs. TAIR 10
Match: AT3G20190.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 454.5 bits (1168), Expect = 1.3e-127
Identity = 240/603 (39.80%), Postives = 368/603 (61.03%), Query Frame = 0

Query: 26  SDNEALLKLRQSFTHAESLNSWDPDSQPC---AARWTGIICHRGVITGLHLTGLRLSGQI 85
           SD + LL+ + +  +A  ++SWDP   PC   +  W G++C  G + GL L G+ L+G++
Sbjct: 51  SDADCLLRFKDTLVNASFISSWDPSISPCKRNSENWFGVLCVTGNVWGLQLEGMGLTGKL 110

Query: 86  DIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLLLTGNDFSGAIPTDFFASLASLK 145
           D+E L  +++LRT+S +NN F G +P     G LKSL L+ N F+G IP D F  +  LK
Sbjct: 111 DLEPLAAIKNLRTLSFMNNKFNGSMPSVKNFGALKSLYLSNNRFTGEIPADAFDGMHHLK 170

Query: 146 KVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIPHLHQAALTSLDLSNNKLEGEIP 205
           K+ L++N F G IP SLA L  L+EL L  NQF G+IP+  Q  L      NN LEG IP
Sbjct: 171 KLLLANNAFRGSIPSSLAYLPMLLELRLNGNQFHGEIPYFKQKDLKLASFENNDLEGPIP 230

Query: 206 ENLAKFDANAFAGNEGLCGKPL-PKSCDGNNAQISEENASSPGESQGSVSKLVVA-SLIA 265
           E+L+  D  +F+GN+ LCG PL P S D  +   S +  SSP E   + S  ++A  LI 
Sbjct: 231 ESLSNMDPVSFSGNKNLCGPPLSPCSSDSGS---SPDLPSSPTEKNKNQSFFIIAIVLIV 290

Query: 266 VAVFLVVVMFLSA--SKRREEKFSKLGREQMNEMVEVHVPSSGHREQLSRKGGDSRRGSQ 325
           + + L+++  +      RR +  S       +   + +   S  ++    K  DS     
Sbjct: 291 IGIILMIISLVVCILHTRRRKSLSAYPSAGQDRTEKYNYDQSTDKD----KAADSVTSYT 350

Query: 326 QGKGGMSD---LVVVNEEKGTFGMADLMKAAAEVLGNGGLGSAYKAVMANGLSVVVKRIR 385
             +G + D   L+ + ++   F + DL++A+AEVLG+G  GS+YK  + +G  +VVKR +
Sbjct: 351 SRRGAVPDQNKLLFLQDDIQRFDLQDLLRASAEVLGSGSFGSSYKTGINSGQMLVVKRYK 410

Query: 386 EMNKLGKDGFDAEMRRMGRLQHHNILTPLAYHYRREEKLLVSEYIPKGSLLYVLHGDRGA 445
            MN +G+D F   MRR+GRL+H N+L  +AY+YRREEKLL++E++P  SL   LH +   
Sbjct: 411 HMNNVGRDEFHEHMRRLGRLKHPNLLPIVAYYYRREEKLLIAEFMPNRSLASHLHANHSV 470

Query: 446 CHAELNWGTRLKIVKGIARGLGFLHSEFATYDLPHGNLKSSNILLSDTYEPLLSDYAFHP 505
               L+W TRLKI++G+A+GLG+L +E  T  +PHG+LKSSN++L +++EPLL+DYA  P
Sbjct: 471 DQPGLDWPTRLKIIQGVAKGLGYLFNELTTLTIPHGHLKSSNVVLDESFEPLLTDYALRP 530

Query: 506 LINPNNATEAMFAYKSPEYAHRQEVSPKSDVYCLGIIILEILTGKFPSQYLSNG-KGGTD 565
           ++N   +   M +YKSPEY+ +  ++ K+DV+CLG++ILE+LTG+FP  YLS G      
Sbjct: 531 VMNSEQSHNLMISYKSPEYSLKGHLTKKTDVWCLGVLILELLTGRFPENYLSQGYDANMS 590

Query: 566 VVQWVSSAVAEKREAELIDPEIANDANAVSRMVELLRIGADCTQSNPQQRPEMKEAIRRI 618
           +V WVS+ V EK+  ++ D E+    N  + M+ LL+IG  C + + ++R EM++A+ +I
Sbjct: 591 LVTWVSNMVKEKKTGDVFDKEMTGKKNCKAEMLNLLKIGLSCCEEDEERRMEMRDAVEKI 646

BLAST of CmaCh12G013330 vs. TAIR 10
Match: AT5G35390.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 451.8 bits (1161), Expect = 8.7e-127
Identity = 257/630 (40.79%), Postives = 382/630 (60.63%), Query Frame = 0

Query: 3   AVRNVFLFLTIFLVGGPPLSCSLSDNEALLKLRQSFT--HAESLNSWDPDSQPCAARWTG 62
           +V NV + L   L+     +  LSD+EA+LK ++S       +L SW+  S PC   W+G
Sbjct: 10  SVYNVMVPLVCLLLFFSTPTHGLSDSEAILKFKESLVVGQENALASWNAKSPPCT--WSG 69

Query: 63  IICHRGVITGLHLTGLRLSGQIDIEALLQLRSLRTVSLVNNSFAGPIPEFNKIGVLKSLL 122
           ++C+ G +  L +  L LSG IDIEAL  L SLRT+S +NN F GP P+F K+  LKSL 
Sbjct: 70  VLCNGGSVWRLQMENLELSGSIDIEALSGLTSLRTLSFMNNKFEGPFPDFKKLAALKSLY 129

Query: 123 LTGNDFSGAIPTDFFASLASLKKVWLSSNNFSGDIPHSLAKLSYLIELHLENNQFSGQIP 182
           L+ N F G IP D F  +  LKKV L+ N F+G IP S+AKL  L+EL L+ NQF+G+IP
Sbjct: 130 LSNNQFGGDIPGDAFEGMGWLKKVHLAQNKFTGQIPSSVAKLPKLLELRLDGNQFTGEIP 189

Query: 183 HL-HQAALTSLDLSNNKLEGEIPENLAKFDANAFAGNEGLCGKPLPKSCDGNNAQISEEN 242
              HQ  L  L+LSNN L G IPE+L+  D   F GN+GL GKPL   CD    +   ++
Sbjct: 190 EFEHQLHL--LNLSNNALTGPIPESLSMTDPKVFEGNKGLYGKPLETECDSPYIEHPPQS 249

Query: 243 ASSPGESQGSVSKLVVASLIAVAVFLVV--VMFL---SASKRREEKFSKLGREQMNEMVE 302
            + P  S  S   LV+ +++A    L++  V+FL   S   ++     + G   + +   
Sbjct: 250 EARPKSS--SRGPLVITAIVAALTILIILGVIFLLNRSYKNKKPRLAVETGPSSLQKKTG 309

Query: 303 VHVPSSGHREQLSRKGGDSRRGSQQGK-----GGMSD--LVVVNEEKGTFGMADLMKAAA 362
           +       R+   RK  D R+GS   K      G+ +  L  + E++  F + DL+KA+A
Sbjct: 310 IREADQSRRD---RKKADHRKGSGTTKRMGAAAGVENTKLSFLREDREKFDLQDLLKASA 369

Query: 363 EVLGNGGLGSAYKAVMANGLSVVVKRIREMNKLGKDGFDAEMRRMGRLQHHNILTPLAYH 422
           E+LG+G  G++YKAV+++G  +VVKR ++MN  G+D F   M+R+GRL HHN+L+ +AY+
Sbjct: 370 EILGSGCFGASYKAVLSSGQMMVVKRFKQMNNAGRDEFQEHMKRLGRLMHHNLLSIVAYY 429

Query: 423 YRREEKLLVSEYIPKGSLLYVLHGDRGACHAELNWGTRLKIVKGIARGLGFLHSEFATYD 482
           YR+EEKLLV ++  +GSL   LH ++      L+W TRLKIVKG+A+GL +LH +  +  
Sbjct: 430 YRKEEKLLVCDFAERGSLAINLHSNQSLGKPSLDWPTRLKIVKGVAKGLFYLHQDLPSLM 489

Query: 483 LPHGNLKSSNILLSDTYEPLLSDYAFHPLINPNNATEAMFAYKSPEYAHRQEVSPKSDVY 542
            PHG+LKSSN+LL+ T+EPLL+DY   PLIN   A   M AY+SPEY   + ++ K+DV+
Sbjct: 490 APHGHLKSSNVLLTKTFEPLLTDYGLIPLINQEKAQMHMAAYRSPEYLQHRRITKKTDVW 549

Query: 543 CLGIIILEILTGKFPSQYLSNGKGGTDVVQWVSSAVAEKREAELIDPEIANDANAVSRMV 602
            LGI+ILEILTGKFP+ +  + +   D+  WV+S         L D  +   ++   +++
Sbjct: 550 GLGILILEILTGKFPANFSQSSE--EDLASWVNSGFHGVWAPSLFDKGMGKTSHCEGQIL 609

Query: 603 ELLRIGADCTQSNPQQRPEMKEAIRRIXEI 618
           +LL IG +C + + ++R ++ +A+ +I E+
Sbjct: 610 KLLTIGLNCCEPDVEKRLDIGQAVEKIEEL 628

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M1L74.0e-18553.08Pollen receptor-like kinase 3 OS=Arabidopsis thaliana OX=3702 GN=PRK3 PE=1 SV=1[more]
Q3E9915.4e-17450.84Pollen receptor-like kinase 6 OS=Arabidopsis thaliana OX=3702 GN=PRK6 PE=1 SV=4[more]
Q9LJY01.9e-12639.80Pollen receptor-like kinase 4 OS=Arabidopsis thaliana OX=3702 GN=PRK4 PE=1 SV=1[more]
C0LGU01.2e-12540.79Pollen receptor-like kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PRK1 PE=1 SV=1[more]
Q9LPT13.0e-12439.52Pollen receptor-like kinase 5 OS=Arabidopsis thaliana OX=3702 GN=PRK5 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HQR80.0e+00100.00pollen receptor-like kinase 3 OS=Cucurbita maxima OX=3661 GN=LOC111465780 PE=4 S... [more]
A0A6J1FAK50.0e+0098.38pollen receptor-like kinase 3 OS=Cucurbita moschata OX=3662 GN=LOC111443910 PE=4... [more]
A0A5D3CFY41.2e-29081.91Pollen receptor-like kinase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3CB951.6e-29081.74pollen receptor-like kinase 3 OS=Cucumis melo OX=3656 GN=LOC103498999 PE=4 SV=1[more]
A0A0A0LIJ23.1e-28981.51Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000... [more]
Match NameE-valueIdentityDescription
XP_022966058.10.0e+00100.00pollen receptor-like kinase 3 [Cucurbita maxima][more]
KAG6586502.10.0e+0098.38Pollen receptor-like kinase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022937521.10.0e+0098.38pollen receptor-like kinase 3 [Cucurbita moschata][more]
XP_023537808.10.0e+0098.06pollen receptor-like kinase 3 [Cucurbita pepo subsp. pepo][more]
KAG7021359.10.0e+0097.57Pollen receptor-like kinase 3, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
Match NameE-valueIdentityDescription
AT3G42880.12.8e-18653.08Leucine-rich repeat protein kinase family protein [more]
AT5G20690.13.8e-17550.84Leucine-rich repeat protein kinase family protein [more]
AT1G72460.16.8e-14846.98Leucine-rich repeat protein kinase family protein [more]
AT3G20190.11.3e-12739.80Leucine-rich repeat protein kinase family protein [more]
AT5G35390.18.7e-12740.79Leucine-rich repeat protein kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001611Leucine-rich repeatPFAMPF13855LRR_8coord: 116..175
e-value: 1.0E-6
score: 28.4
IPR001611Leucine-rich repeatPFAMPF00560LRR_1coord: 187..207
e-value: 0.47
score: 11.2
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 348..612
e-value: 2.3E-28
score: 99.3
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 344..616
score: 27.537991
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 420..616
e-value: 4.8E-39
score: 135.8
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 313..419
e-value: 3.1E-15
score: 57.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 297..315
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 294..325
NoneNo IPR availablePANTHERPTHR48007LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1coord: 4..615
NoneNo IPR availablePANTHERPTHR48007:SF38LEUCINE-RICH REPEAT PROTEIN KINASE FAMILY PROTEINcoord: 4..615
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 350..615
e-value: 3.08393E-86
score: 268.372
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 22..207
IPR013210Leucine-rich repeat-containing N-terminal, plant-typePFAMPF08263LRRNT_2coord: 26..63
e-value: 4.6E-9
score: 36.4
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 185..232
e-value: 1.2E-10
score: 42.2
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 24..184
e-value: 5.7E-34
score: 119.1
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 348..614

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G013330.1CmaCh12G013330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0005515 protein binding
molecular_function GO:0004672 protein kinase activity