CmaCh12G013280 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G013280
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionATP-dependent DNA helicase
LocationCma_Chr12: 10125268 .. 10127802 (-)
RNA-Seq ExpressionCmaCh12G013280
SyntenyCmaCh12G013280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTATGCATTTACTTAATTGTAGTTAGTAAAAATTTGGGCGTGTATGTGTTAGTTTCTGTGCAGGATGAGCATTAACTTTTCTAATTTCATGTTGTTGTCTTACCTAGGGTATAGATAGAAAAGATGTCAGGGTTGTTTGCCACTTCAATATTCCCAAGTCAATGGAAGCTTTTTATCAAGAGTCGGGTAGAGCTGGCCGTGATCAATTACCCTCTAAAAGTCTGTTATACTATGGACTTGAAGACCGCAGAAGAATGGTTAATTTCTGAACTCGACTTGCTGTTTTCTTCCCTCTTTTTCTTTTTGCATTGTTGTTTAACATTTTTCATTGTTACAGGAATTTATTCTAAGGAATAGCTCCAGTGCTGATAAGAAGATTCTACCATCTTCAAGCTCCCAAAAGAAGTTATCGGAGAAATCCTTAACTGACTTTGCTCAGGTGATTTTCATCTGCATTTGTAATCCGACAATGATATTATTTGCCATTAATTTGATGTCCTGGCACCACTGAATGCAGATGGTTGAGTATTGTGAGGGCTCTGGATGTCGCAGGAAGCAAATTCTTGAGAGTTTTGGGGAACAGGTAGGAAGAAGGTCAATTTGTATAATAGGTGTTAGTTTTCTTCCAAACTCTTGAAGTGTCAAAAGCTAATATTATAAATATAACTGTTCGACTGGACTAGGTTCCTGCATCCATATGTAGTAAATCGTGTGATGCCTGCAAACACCCAAATACTGTCGCTGCCTATTTGGAAGAGCTTACTACAACTTGTGCTGTTGGACGAAATAATTCATCTTCTAGAATTTTTGTCAGCAGGTGCTTGACTTAAAAGGCTCTAAATTCTTTTAAATTTTATTATTGAAAAGGGTAGCTGTAAATCTTAGAGGCCACATATTTGATTTAGTTACCAACTCATAATTCTTTTTAAGTAGGGATGGGGATATGAACTTCGGACTTCAAAGAAAAGGGTTGATGTCTTAACCACTAGTCATGCCCACTGCCACTGACTCATAGTTTTTTTTGTTTTTTTGTTTAAAATCAGCTCAAATATGAATGATGAGAAAGAGTTTTCTGAATTTTGGAATCGCAAAGATGAAGCAAGTGGATCTGGTGAAGATATATCTGATTCTGATGGTACAAATCATTTGAATGGACTTGACTTCTCTCCCTCACTTCCAGCCACGTAGCTTAATTTTTGGTTGTATACATTATGTGCTGGGGTTGAATTCGAGTATTTTCTTGTAACTGTGTTTTTTTTATTCTCATTTTCCCCATATTGCCAGATGATACCGAGGTTGTCAAAAGCCTAGCTGGAACGAAGTCTCTAAAGAAATCAGGACTAAATGAAAAGATAGCACTTCTAGAGCGTGCGGAGGAAAATTACTACCAGAACAAGATCTCAGTTAAACAGGTTTGTCTTGATGGTTTCTTTTTCCCTTTGATATTACAATGGGGCGGGAGTTTAGAAATCGAAAACCTAAACCATGCATTTGGTCTAAGGACCGCTTTAGACTGAAAATTCGGGTTGGTTAGATGGGTTATTTTTACTTGACAGTGGATTTATCCATTCATGTTGGATTCATGAACTTGTTTTCCACTATGCTTGCGTCTTTCCTGATGGACTAGATACTTCATGGCAGAATGATAAGCATGACAAGAATGTTGTATCTGCATCATTTAGGGAAGCTAGCAGGAAAAGATTAGAGAATGCTATTGAACAGGCTCAACAACGGCTTGGGAGCTCCAAGTAAGTTAACCGTTCTTTTTCCAATGACAACTTTTAATAATTCCAGTTGCTCTTCTGTCTTTAGACGCTCATTTCCTTGGGATAATCACTTGGCCGATTTTCATTTTGTGTCATACCCACTAAATTCAAATAGGTGGTCGAGATCAATAAGACTAACGGTGATGTAGTTGCTGTTACACTATTCCATGTAATTTCTCTTGGTAACTAACTAGTTCAGTCCTTTGAGACACAAGTGCGTGCATGCTTTGCTCGTAACATGGATGGTAGTTCTGGCAGCATATACATGGTCTTAAGTTTTTCCACAACCACGTATCTTGATTGGAATTCTTGCAGGATTGAAGTGGAAAGATATGCTTGTTTCCTTGAGCATGAGTGCTACGAGAAATATGGAAGAACAGGAAAATCATTCTATTATTCACAAGTGGCCAGTACCATGAGGTGGCTGTCGACAGCAGATTCTAAGGAGTTAACCAGTCGGCTTAGGGCTGAGGAGAATACAGAACTTCCTGCATTGCCATCTCCATCCCCTATGTTAGATCTATCAAAGTCAGAAAAGACTAATGAAGAAGTTCATTGCAGCCCTGTATTGGAAAACCCCGCAAGCAATTTGTCAAAGCAAAATGCTTCCCATGCTGCTGCTGCTGCTCCTCCTTTGCCTCCAATTCCGTCCTTTGCAGAATTTGTTAATGGCAGAATAGAGAAGGCGAATCAATCAAATAAATCACCAAGGCTCACGATTAACAGAGATGAAAAGGATCCAGTAAAGAGGACCAGATTACTGTAG

mRNA sequence

ATGGGTATAGATAGAAAAGATGTCAGGGTTGTTTGCCACTTCAATATTCCCAAGTCAATGGAAGCTTTTTATCAAGAGTCGGGTAGAGCTGGCCGTGATCAATTACCCTCTAAAAGTCTGTTATACTATGGACTTGAAGACCGCAGAAGAATGGAATTTATTCTAAGGAATAGCTCCAGTGCTGATAAGAAGATTCTACCATCTTCAAGCTCCCAAAAGAAGTTATCGGAGAAATCCTTAACTGACTTTGCTCAGATGGTTGAGTATTGTGAGGGCTCTGGATGTCGCAGGAAGCAAATTCTTGAGAGTTTTGGGGAACAGGTTCCTGCATCCATATGTAGTAAATCGTGTGATGCCTGCAAACACCCAAATACTGTCGCTGCCTATTTGGAAGAGCTTACTACAACTTGTGCTGTTGGACGAAATAATTCATCTTCTAGAATTTTTGTCAGCAGCTCAAATATGAATGATGAGAAAGAGTTTTCTGAATTTTGGAATCGCAAAGATGAAGCAAGTGGATCTGGTGAAGATATATCTGATTCTGATGATGATACCGAGGTTGTCAAAAGCCTAGCTGGAACGAAGTCTCTAAAGAAATCAGGACTAAATGAAAAGATAGCACTTCTAGAGCGTGCGGAGGAAAATTACTACCAGAACAAGATCTCAGTTAAACAGAATGATAAGCATGACAAGAATGTTGTATCTGCATCATTTAGGGAAGCTAGCAGGAAAAGATTAGAGAATGCTATTGAACAGGCTCAACAACGGCTTGGGAGCTCCAAGATTGAAGTGGAAAGATATGCTTGTTTCCTTGAGCATGAGTGCTACGAGAAATATGGAAGAACAGGAAAATCATTCTATTATTCACAAGTGGCCAGTACCATGAGGTGGCTGTCGACAGCAGATTCTAAGGAGTTAACCAGTCGGCTTAGGGCTGAGGAGAATACAGAACTTCCTGCATTGCCATCTCCATCCCCTATGTTAGATCTATCAAAGTCAGAAAAGACTAATGAAGAAGTTCATTGCAGCCCTGTATTGGAAAACCCCGCAAGCAATTTGTCAAAGCAAAATGCTTCCCATGCTGCTGCTGCTGCTCCTCCTTTGCCTCCAATTCCGTCCTTTGCAGAATTTGTTAATGGCAGAATAGAGAAGGCGAATCAATCAAATAAATCACCAAGGCTCACGATTAACAGAGATGAAAAGGATCCAGTAAAGAGGACCAGATTACTGTAG

Coding sequence (CDS)

ATGGGTATAGATAGAAAAGATGTCAGGGTTGTTTGCCACTTCAATATTCCCAAGTCAATGGAAGCTTTTTATCAAGAGTCGGGTAGAGCTGGCCGTGATCAATTACCCTCTAAAAGTCTGTTATACTATGGACTTGAAGACCGCAGAAGAATGGAATTTATTCTAAGGAATAGCTCCAGTGCTGATAAGAAGATTCTACCATCTTCAAGCTCCCAAAAGAAGTTATCGGAGAAATCCTTAACTGACTTTGCTCAGATGGTTGAGTATTGTGAGGGCTCTGGATGTCGCAGGAAGCAAATTCTTGAGAGTTTTGGGGAACAGGTTCCTGCATCCATATGTAGTAAATCGTGTGATGCCTGCAAACACCCAAATACTGTCGCTGCCTATTTGGAAGAGCTTACTACAACTTGTGCTGTTGGACGAAATAATTCATCTTCTAGAATTTTTGTCAGCAGCTCAAATATGAATGATGAGAAAGAGTTTTCTGAATTTTGGAATCGCAAAGATGAAGCAAGTGGATCTGGTGAAGATATATCTGATTCTGATGATGATACCGAGGTTGTCAAAAGCCTAGCTGGAACGAAGTCTCTAAAGAAATCAGGACTAAATGAAAAGATAGCACTTCTAGAGCGTGCGGAGGAAAATTACTACCAGAACAAGATCTCAGTTAAACAGAATGATAAGCATGACAAGAATGTTGTATCTGCATCATTTAGGGAAGCTAGCAGGAAAAGATTAGAGAATGCTATTGAACAGGCTCAACAACGGCTTGGGAGCTCCAAGATTGAAGTGGAAAGATATGCTTGTTTCCTTGAGCATGAGTGCTACGAGAAATATGGAAGAACAGGAAAATCATTCTATTATTCACAAGTGGCCAGTACCATGAGGTGGCTGTCGACAGCAGATTCTAAGGAGTTAACCAGTCGGCTTAGGGCTGAGGAGAATACAGAACTTCCTGCATTGCCATCTCCATCCCCTATGTTAGATCTATCAAAGTCAGAAAAGACTAATGAAGAAGTTCATTGCAGCCCTGTATTGGAAAACCCCGCAAGCAATTTGTCAAAGCAAAATGCTTCCCATGCTGCTGCTGCTGCTCCTCCTTTGCCTCCAATTCCGTCCTTTGCAGAATTTGTTAATGGCAGAATAGAGAAGGCGAATCAATCAAATAAATCACCAAGGCTCACGATTAACAGAGATGAAAAGGATCCAGTAAAGAGGACCAGATTACTGTAG

Protein sequence

MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSSADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDACKHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISDSDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFREASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLSTADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASHAAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL
Homology
BLAST of CmaCh12G013280 vs. ExPASy Swiss-Prot
Match: Q9FT72 (ATP-dependent DNA helicase Q-like 3 OS=Arabidopsis thaliana OX=3702 GN=RECQL3 PE=1 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 6.9e-93
Identity = 205/412 (49.76%), Postives = 275/412 (66.75%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MGID+KDVR+VCHFNIPKSME+FYQESGRAGRDQLPS+S+LYYG++DR++ME++LRNS  
Sbjct: 318 MGIDKKDVRMVCHFNIPKSMESFYQESGRAGRDQLPSRSVLYYGVDDRKKMEYLLRNSE- 377

Query: 61  ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 120
            +KK   SSSS+K  S     DF Q+V YCEGSGCRRK+ILESFGE+ P   C K+CDAC
Sbjct: 378 -NKK---SSSSKKPTS-----DFEQIVTYCEGSGCRRKKILESFGEEFPVQQCKKTCDAC 437

Query: 121 KHPNTVAAYLEELTTTCAVGRNNSSSRIFV-SSSNMNDEKEFSEFWNRKDEASGSGEDIS 180
           KHPN VA  LEEL TT +  R ++SSRIF+ SS+N  +E ++SEFWNR ++ S S E+IS
Sbjct: 438 KHPNQVAHCLEELMTTAS--RRHNSSRIFITSSNNKTNEGQYSEFWNRNEDGSNSNEEIS 497

Query: 181 DSDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFR 240
           DSDD TE   S+ G K  KK GL+EK+ LLE+AEE YY+     KQ  K +KN +S + R
Sbjct: 498 DSDDATEAANSVTGPKLSKKLGLDEKLVLLEQAEEKYYERN---KQVKKSEKNAISEALR 557

Query: 241 EASRKRLENAIEQAQQRLGS-SKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWL 300
           ++S++RL +A+ +  Q L    +I+ ++ + FLE+ECY KY + GKSFYYSQ+AST+RWL
Sbjct: 558 DSSKQRLLDALTRVLQLLACVEEIDSQKGSEFLENECYRKYSKAGKSFYYSQIASTVRWL 617

Query: 301 STADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVH-CSPVLENPASNLSKQN 360
            TA   EL +RL +  +      P   P+L     E   EE    +  +E+     +++ 
Sbjct: 618 GTASRDELMTRLSSVVSLAREQEPLEEPLLATEPVENIEEEDDGKTNTVESRVDEPTQEL 677

Query: 361 ASHAAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRL 410
                 +   LP +PSF+EFVN R  K  Q+    + +   D+K P K  +L
Sbjct: 678 VVSPILSPIRLPQVPSFSEFVNRR--KIKQNTLIDKSSEGFDDKKPAKIMKL 712

BLAST of CmaCh12G013280 vs. ExPASy Swiss-Prot
Match: O94762 (ATP-dependent DNA helicase Q5 OS=Homo sapiens OX=9606 GN=RECQL5 PE=1 SV=2)

HSP 1 Score: 101.3 bits (251), Expect = 2.7e-20
Identity = 86/286 (30.07%), Postives = 136/286 (47.55%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MG+D+ +VR V H+NI KSM  +YQESGRAGRD  PS   LYY   DR ++ F++R   +
Sbjct: 321 MGVDKANVRFVAHWNIAKSMAGYYQESGRAGRDGKPSWCRLYYSRNDRDQVSFLIRKEVA 380

Query: 61  ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 120
                L      K   + ++  F  +V +CE  GCR   I + FG+ +PA  C+K CD C
Sbjct: 381 K----LQEKRGNKASDKATIMAFDALVTFCEELGCRHAAIAKYFGDALPA--CAKGCDHC 440

Query: 121 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMN---------DEKEFSEFWNRKDEA 180
           ++P  V   LE      A+ R++S S+  +  S  N           K + +F    + +
Sbjct: 441 QNPTAVRRRLE------ALERSSSWSKTCIGPSQGNGFDPELYEGGRKGYGDFSRYDEGS 500

Query: 181 SGSGEDISDSDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDK 240
            GSG++  D     E          L+K G + KI      +EN    + S ++  +   
Sbjct: 501 GGSGDEGRDEAHKREWNLFYQKQMQLRK-GKDPKIEEFVPPDENCPLKEASSRRIPR--- 560

Query: 241 NVVSASFREASRKRLENAIEQAQQRLGSS-KIEVERYACFLEHECY 277
             ++   RE   + LE A+   +Q   ++ + ++   A  LEHE +
Sbjct: 561 --LTVKAREHCLRLLEEALSSNRQSTRTADEADLRAKAVELEHETF 588

BLAST of CmaCh12G013280 vs. ExPASy Swiss-Prot
Match: Q8VID5 (ATP-dependent DNA helicase Q5 OS=Mus musculus OX=10090 GN=Recql5 PE=1 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 4.6e-20
Identity = 92/286 (32.17%), Postives = 138/286 (48.25%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MG+D+ +VR V H+NI KSM  +YQESGRAGRD  PS   LYY   DR ++ F++R   +
Sbjct: 322 MGVDKANVRFVAHWNIAKSMAGYYQESGRAGRDGKPSWCRLYYSRNDRDQVSFLIRKELA 381

Query: 61  ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 120
                L      K   + +L  F  +V +CE  GCR   I + FG+  PA  C+K CD C
Sbjct: 382 K----LQEKRGNKPSDKATLLAFDALVTFCEEVGCRHAAIAKYFGDAPPA--CAKGCDYC 441

Query: 121 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMND-EKEFSE-------FWNRKDEAS 180
           ++P  +   L+      A+ R++S S+  +  S  N  + E  E        ++R DE S
Sbjct: 442 QNPAAITKKLD------ALERSSSWSKTCIGPSQGNGFDPELYEGGRRGYGGFSRYDEGS 501

Query: 181 -GSGEDISDSDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDK 240
            GSG++  D     E         SL+K G   KI      +E+    + S ++  K   
Sbjct: 502 GGSGDEGRDEAHKREWNLFYQKQMSLRK-GKEPKIEEFTPPDEDCPLREASSRKIPK--- 561

Query: 241 NVVSASFREASRKRLENAIEQAQQRLGSSK-IEVERYACFLEHECY 277
             ++   RE   + LE A+    Q  GS+   +++  A  LEHE +
Sbjct: 562 --LTVKAREHCLRLLEEALISNHQAAGSTHGADLQAKAVELEHETF 589

BLAST of CmaCh12G013280 vs. ExPASy Swiss-Prot
Match: D4ACP5 (ATP-dependent DNA helicase Q5 OS=Rattus norvegicus OX=10116 GN=Recql5 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 4.3e-18
Identity = 82/282 (29.08%), Postives = 124/282 (43.97%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MG+D+ +VR V H+NI KSM  +YQESGRAGRD  PS   LYY   DR ++ F++R   +
Sbjct: 322 MGVDKANVRFVAHWNIAKSMAGYYQESGRAGRDGKPSWCRLYYSRNDRDQVSFLIRKELA 381

Query: 61  ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 120
                L      K   + +L  F  +V +CE  GCR   I + FG+  PA  C+K CD C
Sbjct: 382 K----LQEKRGNKPSDKATLLAFDALVTFCEEVGCRHAAIAKYFGDAPPA--CAKGCDCC 441

Query: 121 KHPNTVAAYLEELTTTCAVGR---NNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGED 180
           + P  +   L+ L  + + G+     S    F         + +  F    + + GSG++
Sbjct: 442 QSPAAIRKKLDALEHSSSWGKTCIGPSQGDGFDPELYEGGRRGYGGFSRYDEGSGGSGDE 501

Query: 181 ISDSDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNV--VS 240
             D     E         SL+K          E   E +          D   + +  ++
Sbjct: 502 GRDEAHKREWNLFYQRQMSLRKG--------KEAKPEEFTPPGEDCPLRDASSRKIPKLT 561

Query: 241 ASFREASRKRLENAIEQAQQRLGSSK-IEVERYACFLEHECY 277
              RE   + LE A+    Q  GS+   +++  A  LEHE +
Sbjct: 562 VKAREHCLRLLEEALNSNHQAAGSTHGADLQAKAVELEHETF 589

BLAST of CmaCh12G013280 vs. ExPASy Swiss-Prot
Match: P35187 (ATP-dependent helicase SGS1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SGS1 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 4.3e-18
Identity = 46/127 (36.22%), Postives = 72/127 (56.69%), Query Frame = 0

Query: 1    MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
            MGID+ DVR V HF +P+++E +YQE+GRAGRD   S  + Y+   D R M+ +++   +
Sbjct: 962  MGIDKPDVRFVYHFTVPRTLEGYYQETGRAGRDGNYSYCITYFSFRDIRTMQTMIQKDKN 1021

Query: 61   ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEG-SGCRRKQILESFGEQVPASICSKSCDA 120
             D          ++  EK L    Q++ YC+  + CRRK +L  F E   + +C K+CD 
Sbjct: 1022 LD----------RENKEKHLNKLQQVMAYCDNVTDCRRKLVLSYFNEDFDSKLCHKNCDN 1078

Query: 121  CKHPNTV 127
            C++   V
Sbjct: 1082 CRNSANV 1078

BLAST of CmaCh12G013280 vs. ExPASy TrEMBL
Match: A0A6J1HM01 (ATP-dependent DNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111465781 PE=3 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 1.4e-224
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS
Sbjct: 174 MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 233

Query: 61  ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 120
           ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC
Sbjct: 234 ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 293

Query: 121 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD 180
           KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD
Sbjct: 294 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD 353

Query: 181 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE 240
           SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE
Sbjct: 354 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE 413

Query: 241 ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST 300
           ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST
Sbjct: 414 ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST 473

Query: 301 ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH 360
           ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH
Sbjct: 474 ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH 533

Query: 361 AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL 411
           AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL
Sbjct: 534 AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL 583

BLAST of CmaCh12G013280 vs. ExPASy TrEMBL
Match: A0A6J1HSL2 (ATP-dependent DNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111465781 PE=3 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 1.4e-224
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS
Sbjct: 318 MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 377

Query: 61  ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 120
           ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC
Sbjct: 378 ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 437

Query: 121 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD 180
           KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD
Sbjct: 438 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD 497

Query: 181 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE 240
           SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE
Sbjct: 498 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE 557

Query: 241 ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST 300
           ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST
Sbjct: 558 ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST 617

Query: 301 ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH 360
           ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH
Sbjct: 618 ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH 677

Query: 361 AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL 411
           AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL
Sbjct: 678 AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL 727

BLAST of CmaCh12G013280 vs. ExPASy TrEMBL
Match: A0A6J1HQJ2 (ATP-dependent DNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111465781 PE=3 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 1.4e-224
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS
Sbjct: 230 MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 289

Query: 61  ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 120
           ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC
Sbjct: 290 ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 349

Query: 121 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD 180
           KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD
Sbjct: 350 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD 409

Query: 181 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE 240
           SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE
Sbjct: 410 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE 469

Query: 241 ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST 300
           ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST
Sbjct: 470 ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST 529

Query: 301 ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH 360
           ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH
Sbjct: 530 ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH 589

Query: 361 AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL 411
           AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL
Sbjct: 590 AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL 639

BLAST of CmaCh12G013280 vs. ExPASy TrEMBL
Match: A0A6J1FC10 (ATP-dependent DNA helicase OS=Cucurbita moschata OX=3662 GN=LOC111444040 PE=3 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 1.8e-216
Identity = 400/410 (97.56%), Postives = 403/410 (98.29%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS
Sbjct: 318 MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 377

Query: 61  ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 120
           ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPA ICSKSCDAC
Sbjct: 378 ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPAFICSKSCDAC 437

Query: 121 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD 180
           KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGS EDISD
Sbjct: 438 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSEEDISD 497

Query: 181 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE 240
           SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDK DKNVVSASFRE
Sbjct: 498 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKQDKNVVSASFRE 557

Query: 241 ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST 300
           ASRKRLENAIEQAQQRLGSS+IEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST
Sbjct: 558 ASRKRLENAIEQAQQRLGSSRIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST 617

Query: 301 ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH 360
           ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNAS 
Sbjct: 618 ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASP 677

Query: 361 AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL 411
           AAAA  PLPPIPSFAEFVNGR+EKAN SNKSPRL+INRDEKDPVKRTRLL
Sbjct: 678 AAAA--PLPPIPSFAEFVNGRMEKANHSNKSPRLSINRDEKDPVKRTRLL 725

BLAST of CmaCh12G013280 vs. ExPASy TrEMBL
Match: A0A6J1FB65 (ATP-dependent DNA helicase OS=Cucurbita moschata OX=3662 GN=LOC111444040 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.0e-215
Identity = 401/411 (97.57%), Postives = 403/411 (98.05%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS
Sbjct: 174 MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 233

Query: 61  ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 120
           ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPA ICSKSCDAC
Sbjct: 234 ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPAFICSKSCDAC 293

Query: 121 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD 180
           KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGS EDISD
Sbjct: 294 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSEEDISD 353

Query: 181 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE 240
           SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDK DKNVVSASFRE
Sbjct: 354 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKQDKNVVSASFRE 413

Query: 241 ASRKRLENAIEQAQQRLGSS-KIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLS 300
           ASRKRLENAIEQAQQRLGSS KIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLS
Sbjct: 414 ASRKRLENAIEQAQQRLGSSFKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLS 473

Query: 301 TADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNAS 360
           TADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNAS
Sbjct: 474 TADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNAS 533

Query: 361 HAAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL 411
            AAAA  PLPPIPSFAEFVNGR+EKAN SNKSPRL+INRDEKDPVKRTRLL
Sbjct: 534 PAAAA--PLPPIPSFAEFVNGRMEKANHSNKSPRLSINRDEKDPVKRTRLL 582

BLAST of CmaCh12G013280 vs. NCBI nr
Match: XP_022966061.1 (ATP-dependent DNA helicase Q-like 3 isoform X3 [Cucurbita maxima] >XP_022966062.1 ATP-dependent DNA helicase Q-like 3 isoform X3 [Cucurbita maxima])

HSP 1 Score: 788.5 bits (2035), Expect = 2.8e-224
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS
Sbjct: 174 MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 233

Query: 61  ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 120
           ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC
Sbjct: 234 ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 293

Query: 121 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD 180
           KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD
Sbjct: 294 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD 353

Query: 181 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE 240
           SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE
Sbjct: 354 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE 413

Query: 241 ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST 300
           ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST
Sbjct: 414 ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST 473

Query: 301 ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH 360
           ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH
Sbjct: 474 ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH 533

Query: 361 AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL 411
           AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL
Sbjct: 534 AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL 583

BLAST of CmaCh12G013280 vs. NCBI nr
Match: XP_022966060.1 (ATP-dependent DNA helicase Q-like 3 isoform X2 [Cucurbita maxima])

HSP 1 Score: 788.5 bits (2035), Expect = 2.8e-224
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS
Sbjct: 230 MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 289

Query: 61  ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 120
           ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC
Sbjct: 290 ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 349

Query: 121 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD 180
           KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD
Sbjct: 350 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD 409

Query: 181 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE 240
           SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE
Sbjct: 410 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE 469

Query: 241 ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST 300
           ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST
Sbjct: 470 ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST 529

Query: 301 ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH 360
           ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH
Sbjct: 530 ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH 589

Query: 361 AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL 411
           AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL
Sbjct: 590 AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL 639

BLAST of CmaCh12G013280 vs. NCBI nr
Match: XP_022966059.1 (ATP-dependent DNA helicase Q-like 3 isoform X1 [Cucurbita maxima])

HSP 1 Score: 788.5 bits (2035), Expect = 2.8e-224
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS
Sbjct: 318 MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 377

Query: 61  ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 120
           ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC
Sbjct: 378 ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 437

Query: 121 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD 180
           KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD
Sbjct: 438 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD 497

Query: 181 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE 240
           SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE
Sbjct: 498 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE 557

Query: 241 ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST 300
           ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST
Sbjct: 558 ASRKRLENAIEQAQQRLGSSKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLST 617

Query: 301 ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH 360
           ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH
Sbjct: 618 ADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNASH 677

Query: 361 AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL 411
           AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL
Sbjct: 678 AAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL 727

BLAST of CmaCh12G013280 vs. NCBI nr
Match: XP_023537807.1 (ATP-dependent DNA helicase Q-like 3 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 767.7 bits (1981), Expect = 5.1e-218
Identity = 405/412 (98.30%), Postives = 407/412 (98.79%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS
Sbjct: 230 MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 289

Query: 61  ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 120
           ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC
Sbjct: 290 ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 349

Query: 121 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD 180
           KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD
Sbjct: 350 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD 409

Query: 181 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE 240
           SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDK DKNVVSASFRE
Sbjct: 410 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKQDKNVVSASFRE 469

Query: 241 ASRKRLENAIEQAQQRLGSS-KIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLS 300
           ASRKRLENAIEQAQQRLGSS KIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLS
Sbjct: 470 ASRKRLENAIEQAQQRLGSSFKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLS 529

Query: 301 TADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNAS 360
           TADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNAS
Sbjct: 530 TADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNAS 589

Query: 361 -HAAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL 411
             AAAAAPPLPPIPSFAEFVNGR+EKAN SNKSPRL+INRDEKDPVKRTRLL
Sbjct: 590 PAAAAAAPPLPPIPSFAEFVNGRMEKANHSNKSPRLSINRDEKDPVKRTRLL 641

BLAST of CmaCh12G013280 vs. NCBI nr
Match: XP_023537806.1 (ATP-dependent DNA helicase Q-like 3 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 767.7 bits (1981), Expect = 5.1e-218
Identity = 405/412 (98.30%), Postives = 407/412 (98.79%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS
Sbjct: 318 MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 377

Query: 61  ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 120
           ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC
Sbjct: 378 ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 437

Query: 121 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD 180
           KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD
Sbjct: 438 KHPNTVAAYLEELTTTCAVGRNNSSSRIFVSSSNMNDEKEFSEFWNRKDEASGSGEDISD 497

Query: 181 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFRE 240
           SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDK DKNVVSASFRE
Sbjct: 498 SDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKQDKNVVSASFRE 557

Query: 241 ASRKRLENAIEQAQQRLGSS-KIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLS 300
           ASRKRLENAIEQAQQRLGSS KIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLS
Sbjct: 558 ASRKRLENAIEQAQQRLGSSFKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWLS 617

Query: 301 TADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNAS 360
           TADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNAS
Sbjct: 618 TADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVHCSPVLENPASNLSKQNAS 677

Query: 361 -HAAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRLL 411
             AAAAAPPLPPIPSFAEFVNGR+EKAN SNKSPRL+INRDEKDPVKRTRLL
Sbjct: 678 PAAAAAAPPLPPIPSFAEFVNGRMEKANHSNKSPRLSINRDEKDPVKRTRLL 729

BLAST of CmaCh12G013280 vs. TAIR 10
Match: AT4G35740.1 (DEAD/DEAH box RNA helicase family protein )

HSP 1 Score: 342.4 bits (877), Expect = 4.9e-94
Identity = 205/412 (49.76%), Postives = 275/412 (66.75%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MGID+KDVR+VCHFNIPKSME+FYQESGRAGRDQLPS+S+LYYG++DR++ME++LRNS  
Sbjct: 318 MGIDKKDVRMVCHFNIPKSMESFYQESGRAGRDQLPSRSVLYYGVDDRKKMEYLLRNSE- 377

Query: 61  ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 120
            +KK   SSSS+K  S     DF Q+V YCEGSGCRRK+ILESFGE+ P   C K+CDAC
Sbjct: 378 -NKK---SSSSKKPTS-----DFEQIVTYCEGSGCRRKKILESFGEEFPVQQCKKTCDAC 437

Query: 121 KHPNTVAAYLEELTTTCAVGRNNSSSRIFV-SSSNMNDEKEFSEFWNRKDEASGSGEDIS 180
           KHPN VA  LEEL TT +  R ++SSRIF+ SS+N  +E ++SEFWNR ++ S S E+IS
Sbjct: 438 KHPNQVAHCLEELMTTAS--RRHNSSRIFITSSNNKTNEGQYSEFWNRNEDGSNSNEEIS 497

Query: 181 DSDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFR 240
           DSDD TE   S+ G K  KK GL+EK+ LLE+AEE YY+     KQ  K +KN +S + R
Sbjct: 498 DSDDATEAANSVTGPKLSKKLGLDEKLVLLEQAEEKYYERN---KQVKKSEKNAISEALR 557

Query: 241 EASRKRLENAIEQAQQRLGS-SKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWL 300
           ++S++RL +A+ +  Q L    +I+ ++ + FLE+ECY KY + GKSFYYSQ+AST+RWL
Sbjct: 558 DSSKQRLLDALTRVLQLLACVEEIDSQKGSEFLENECYRKYSKAGKSFYYSQIASTVRWL 617

Query: 301 STADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVH-CSPVLENPASNLSKQN 360
            TA   EL +RL +  +      P   P+L     E   EE    +  +E+     +++ 
Sbjct: 618 GTASRDELMTRLSSVVSLAREQEPLEEPLLATEPVENIEEEDDGKTNTVESRVDEPTQEL 677

Query: 361 ASHAAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRL 410
                 +   LP +PSF+EFVN R  K  Q+    + +   D+K P K  +L
Sbjct: 678 VVSPILSPIRLPQVPSFSEFVNRR--KIKQNTLIDKSSEGFDDKKPAKIMKL 712

BLAST of CmaCh12G013280 vs. TAIR 10
Match: AT4G35740.2 (DEAD/DEAH box RNA helicase family protein )

HSP 1 Score: 342.4 bits (877), Expect = 4.9e-94
Identity = 205/412 (49.76%), Postives = 275/412 (66.75%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MGID+KDVR+VCHFNIPKSME+FYQESGRAGRDQLPS+S+LYYG++DR++ME++LRNS  
Sbjct: 225 MGIDKKDVRMVCHFNIPKSMESFYQESGRAGRDQLPSRSVLYYGVDDRKKMEYLLRNSE- 284

Query: 61  ADKKILPSSSSQKKLSEKSLTDFAQMVEYCEGSGCRRKQILESFGEQVPASICSKSCDAC 120
            +KK   SSSS+K  S     DF Q+V YCEGSGCRRK+ILESFGE+ P   C K+CDAC
Sbjct: 285 -NKK---SSSSKKPTS-----DFEQIVTYCEGSGCRRKKILESFGEEFPVQQCKKTCDAC 344

Query: 121 KHPNTVAAYLEELTTTCAVGRNNSSSRIFV-SSSNMNDEKEFSEFWNRKDEASGSGEDIS 180
           KHPN VA  LEEL TT +  R ++SSRIF+ SS+N  +E ++SEFWNR ++ S S E+IS
Sbjct: 345 KHPNQVAHCLEELMTTAS--RRHNSSRIFITSSNNKTNEGQYSEFWNRNEDGSNSNEEIS 404

Query: 181 DSDDDTEVVKSLAGTKSLKKSGLNEKIALLERAEENYYQNKISVKQNDKHDKNVVSASFR 240
           DSDD TE   S+ G K  KK GL+EK+ LLE+AEE YY+     KQ  K +KN +S + R
Sbjct: 405 DSDDATEAANSVTGPKLSKKLGLDEKLVLLEQAEEKYYERN---KQVKKSEKNAISEALR 464

Query: 241 EASRKRLENAIEQAQQRLGS-SKIEVERYACFLEHECYEKYGRTGKSFYYSQVASTMRWL 300
           ++S++RL +A+ +  Q L    +I+ ++ + FLE+ECY KY + GKSFYYSQ+AST+RWL
Sbjct: 465 DSSKQRLLDALTRVLQLLACVEEIDSQKGSEFLENECYRKYSKAGKSFYYSQIASTVRWL 524

Query: 301 STADSKELTSRLRAEENTELPALPSPSPMLDLSKSEKTNEEVH-CSPVLENPASNLSKQN 360
            TA   EL +RL +  +      P   P+L     E   EE    +  +E+     +++ 
Sbjct: 525 GTASRDELMTRLSSVVSLAREQEPLEEPLLATEPVENIEEEDDGKTNTVESRVDEPTQEL 584

Query: 361 ASHAAAAAPPLPPIPSFAEFVNGRIEKANQSNKSPRLTINRDEKDPVKRTRL 410
                 +   LP +PSF+EFVN R  K  Q+    + +   D+K P K  +L
Sbjct: 585 VVSPILSPIRLPQVPSFSEFVNRR--KIKQNTLIDKSSEGFDDKKPAKIMKL 619

BLAST of CmaCh12G013280 vs. TAIR 10
Match: AT3G05740.1 (RECQ helicase l1 )

HSP 1 Score: 83.6 bits (205), Expect = 4.1e-16
Identity = 49/125 (39.20%), Postives = 70/125 (56.00%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MGID+ DVR V H  + K++E++YQESGRAGRD L ++ +  Y  +D  R+  +LRN   
Sbjct: 490 MGIDKADVRFVIHNTLSKAVESYYQESGRAGRDGLQAQCICLYQKKDFSRVVCMLRNGQG 549

Query: 61  ADKKILPSSSSQKKLSEKSLTDFAQMVEYCE-GSGCRRKQILESFGEQVPASICSKS--- 120
            +     S+ +Q K          +M +YCE  + CRR+ +LE FGE     IC  S   
Sbjct: 550 RNMDRFKSAMAQAK----------KMQQYCELKTECRRQMLLEYFGESFDRMICKSSLNP 604

Query: 121 CDACK 122
           CD C+
Sbjct: 610 CDNCE 604

BLAST of CmaCh12G013280 vs. TAIR 10
Match: AT1G10930.1 (DNA helicase (RECQl4A) )

HSP 1 Score: 82.8 bits (203), Expect = 7.0e-16
Identity = 47/125 (37.60%), Postives = 72/125 (57.60%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MGI++ DVR V H ++PKS+E ++QE GRAGRD   S  +LYYG  D  R++ ++ +   
Sbjct: 735 MGINKPDVRFVIHHSLPKSIEGYHQECGRAGRDGQRSSCVLYYGYGDYIRVKHMI-SQGG 794

Query: 61  ADKKILPSS----SSQKKLSEKSLTDFAQMVEYCEGS-GCRRKQILESFGEQVPASICSK 120
            D+  + +     +S  +L E +  +  +MV YCE    CRR   L   GE+  ++ C K
Sbjct: 795 VDQSPMATGYNRVASSGRLLETNTENLLRMVRYCENEVECRRFLQLVHLGEKFDSTNCKK 854

BLAST of CmaCh12G013280 vs. TAIR 10
Match: AT1G60930.1 (RECQ helicase L4B )

HSP 1 Score: 78.2 bits (191), Expect = 1.7e-14
Identity = 44/124 (35.48%), Postives = 66/124 (53.23%), Query Frame = 0

Query: 1   MGIDRKDVRVVCHFNIPKSMEAFYQESGRAGRDQLPSKSLLYYGLEDRRRMEFILRNSSS 60
           MGI++ DVR V H ++PKS+E ++QE GRAGRD   S  +LYY   D  R++ ++     
Sbjct: 751 MGINKPDVRFVIHHSLPKSIEGYHQECGRAGRDGQRSSCVLYYSYTDYIRVKHMISQGGL 810

Query: 61  AD---KKILPSSSSQKKLSEKSLTDFAQMVEYCEGS-GCRRKQILESFGEQVPASICSKS 120
                K      +S  ++ E +  +  +MV YCE    CRR   L   GE+  ++ C  +
Sbjct: 811 GQGQMKMGYNCKASSGRMLETNTENLLRMVSYCENEVDCRRFLQLVHLGEKFDSTNCKNT 870

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FT726.9e-9349.76ATP-dependent DNA helicase Q-like 3 OS=Arabidopsis thaliana OX=3702 GN=RECQL3 PE... [more]
O947622.7e-2030.07ATP-dependent DNA helicase Q5 OS=Homo sapiens OX=9606 GN=RECQL5 PE=1 SV=2[more]
Q8VID54.6e-2032.17ATP-dependent DNA helicase Q5 OS=Mus musculus OX=10090 GN=Recql5 PE=1 SV=1[more]
D4ACP54.3e-1829.08ATP-dependent DNA helicase Q5 OS=Rattus norvegicus OX=10116 GN=Recql5 PE=1 SV=1[more]
P351874.3e-1836.22ATP-dependent helicase SGS1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S2... [more]
Match NameE-valueIdentityDescription
A0A6J1HM011.4e-224100.00ATP-dependent DNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111465781 PE=3 SV=1[more]
A0A6J1HSL21.4e-224100.00ATP-dependent DNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111465781 PE=3 SV=1[more]
A0A6J1HQJ21.4e-224100.00ATP-dependent DNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111465781 PE=3 SV=1[more]
A0A6J1FC101.8e-21697.56ATP-dependent DNA helicase OS=Cucurbita moschata OX=3662 GN=LOC111444040 PE=3 SV... [more]
A0A6J1FB652.0e-21597.57ATP-dependent DNA helicase OS=Cucurbita moschata OX=3662 GN=LOC111444040 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_022966061.12.8e-224100.00ATP-dependent DNA helicase Q-like 3 isoform X3 [Cucurbita maxima] >XP_022966062.... [more]
XP_022966060.12.8e-224100.00ATP-dependent DNA helicase Q-like 3 isoform X2 [Cucurbita maxima][more]
XP_022966059.12.8e-224100.00ATP-dependent DNA helicase Q-like 3 isoform X1 [Cucurbita maxima][more]
XP_023537807.15.1e-21898.30ATP-dependent DNA helicase Q-like 3 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023537806.15.1e-21898.30ATP-dependent DNA helicase Q-like 3 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G35740.14.9e-9449.76DEAD/DEAH box RNA helicase family protein [more]
AT4G35740.24.9e-9449.76DEAD/DEAH box RNA helicase family protein [more]
AT3G05740.14.1e-1639.20RECQ helicase l1 [more]
AT1G10930.17.0e-1637.60DNA helicase (RECQl4A) [more]
AT1G60930.11.7e-1435.48RECQ helicase L4B [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 236..263
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 308..370
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 394..410
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 382..410
NoneNo IPR availablePANTHERPTHR13710DNA HELICASE RECQ FAMILY MEMBERcoord: 1..389
NoneNo IPR availablePANTHERPTHR13710:SF134ATP-DEPENDENT DNA HELICASE Q-LIKE 3coord: 1..389
IPR032284ATP-dependent DNA helicase RecQ, zinc-binding domainPFAMPF16124RecQ_Zn_bindcoord: 46..121
e-value: 8.5E-14
score: 52.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 1..139
e-value: 8.2E-34
score: 119.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 1..131
IPR001650Helicase, C-terminalPROSITEPS51194HELICASE_CTERcoord: 1..81
score: 10.205186

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G013280.1CmaCh12G013280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034605 cellular response to heat
biological_process GO:0032508 DNA duplex unwinding
biological_process GO:0006310 DNA recombination
biological_process GO:0006357 regulation of transcription by RNA polymerase II
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0003678 DNA helicase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding