CmaCh12G013250 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G013250
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionRING-box protein 1a-like
LocationCma_Chr12: 10120293 .. 10122405 (+)
RNA-Seq ExpressionCmaCh12G013250
SyntenyCmaCh12G013250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACTCCGACGTGACGATGGTTCCGGTCGGCGAGGCTTCGAGCAGTGCCGGTCCTTCGTCTACCAAGAAACCCAAGCGTTTTGAGATCAAAAAGTGGAACGCCGTCGCTCTATGGGCATGGGGTAATTACGGAACCCTAATCTTTAGCCCTTCACACGGCGTTGCTATTACTGCTCTCACTCTCCCGTTTGCCTTCTTACATCGGAATCCTCCTCTCTAATTTTTGTGTTATTCATTTCTTAATTCAGATATCGTTGTCGATAACTGTGCGATCTGCAGAAATCATATCATGGATCTTTGTAAGTTTATCATCATTTGATCAAGTTCTAAGATATCTGGAATGATCTTCCTCTTAATTTTCTATATTATTTGTATTTTCTTTAGCCATTCACCATTGTTTGTTATGTAGGCATCGAGTGCCAAGCAAACCAGGCAAGCGCAACCAGCGAGGAATGTACTGTGGCTTGGGGTACGTTTTTTTTTTTTATAAAAACATTTCCTCTCCCTATCCCCAATCCATTCACGCAGACACAATGTGCTATTATTTTCTACCTCACAACTTACGGATACTGAGGTGGATGATTGGGCCTCTATGCTGAATATTTAAAGCCATGTCATATTTTCTAATTAGGAGGATGTCGAAGTGCGTTCCCTTCATATTAATGGTTGCTTTTCTGTCAATTCCTCAGACCTTTAGCTTGTTTGTTAACATAGCCATACTGACCTAATCCGTAGTAAGCTAACCTGGGAGGCTAAAATTCTGAAAAAGTGAGAAGCGTTCCGAATGGCTGGTGATGTATCCTGTGCAGGAAAGACAAGAGAGCCATTTCATGTTTTTGTCCACTGCCCATTTTCTGCCGCTATTTGTTGAGCTAAGCATTGTAATGTATTTGGTATGAGTTGATGCTCCCGTAAGACCTCTGAGAATTACTGCCATCCAACTGTTAGGGCACAATCTAAAGGTCAAGTTATAATCAGAGACTTTCTAAGGGGGTTGATTCTAGCTCGGAGAGCTTTTGGGACCTCAGGAAACTCTATTAAGAACTGAACTTATTATTTAACTTAAAAAGATTGCCTTTTGGACTGATTGATTATTACTCTATCCAACTTACAGTATGCAACATTATTTTGCAATTTCCCTTGATGTGGAATTATAGCTGGAGCCAACGACTGATGATACCTGGTGTTGAATTTTTATATATTTGTAGGGGTGTGTAATCATGCATTTCATTTCCACTGTATCAGTAGATGGTTGAAGACGCGTCAAGTTTGCCCATTGGGTATGCATTTCACATTCTTGCCCAGCCTTTCAACCACAATTTCTTTTCTAAAAAAATGAATTTTGGTAATTGAAGAAAGCTTTGCCATTTTACAGATAATAGCGAGTGGGAATTTCAGAAGTACGGTCACTAGAACTTTGCTGCTGAGAATATGGATCCTTAAATTCTCATGGGTCTCTAATGTTGTCCCCGATGAACTTTCTCCATACATGGTTTCAGAAATTTGCGATTCGAAGATGCATTTTGTTGAGCAACTACAATTGTCCAGAGAATTATGTGATTTTATTTGGGAGTTGTTTGTAGAAATAGGCTAGCACTCGAACGTTAGTTCTTGTTTTACCGTTTACCATTTCTTTACTTGTATTTATGTTAACATTTCACTCGGTTCATCTGGAAAATTTGGATCTTATTGTTGCCTATTTGCTCTTCGATTCTTGTTTGTCTATCGGATATTCTTCTTCCCATAATATAATCGTGTTCTTCACCGAATGAATGCTTCTAAAGTGGTCTTACTTTAGAATGATTGTTGGCTGCTCTGAATGAAAAATACCAGTAGGCTGTAACGATGAGAAAATATAACATCATGGATTTGGTTGATATTCAGAACAGGTTAAAATTGTTATTTTGTTTTTCTTCTGAATATATTGCATGATGACTATGTTTTTCTTCTGAATTGTTAACTGGCACTTTTGTGTTGCTATCGAACTGTGCTGAGCTCTCTCAGTTTGTGCAGGCAAGGAGTTTGGGATGCCCATTCTTCTCACTTTGTTGCAGCCGGACGCACCAGTTTTTGCTGATTGCCCCGCACCTCACACAGTTAACAGTTGCTAA

mRNA sequence

ATGGACTCCGACGTGACGATGGTTCCGGTCGGCGAGGCTTCGAGCAGTGCCGGTCCTTCGTCTACCAAGAAACCCAAGCGTTTTGAGATCAAAAAGTGGAACGCCGTCGCTCTATGGGCATGGGATATCGTTGTCGATAACTGTGCGATCTGCAGAAATCATATCATGGATCTTTGCATCGAGTGCCAAGCAAACCAGGCAAGCGCAACCAGCGAGGAATGTACTGTGGCTTGGGACACAATGTGCTATTATTTTCTACCTCACAACTTACGGATACTGAGGAGGATGTCGAACCATACTGACCTAATCCGTAGGGTGTGTAATCATGCATTTCATTTCCACTGTATCAGTAGATGGTTGAAGACGCGTCAAGTTTGCCCATTGGAAAGCTTTGCCATTTTACAGATAATAGCGAGTGGGAATTTCAGAAGTACGGTCACTAGAACTTTGCTGCTGAGAATATGGATCCTTAAATTCTCATGGGTCTCTAATGTTGTCCCCGATGAACTTTCTCCATACATGGTTTCAGAAATTTGCGATTCGAAGATGCATTTTGTTGAGCAACTACAATTGTCCAGAGAATTATTTTGTGCAGGCAAGGAGTTTGGGATGCCCATTCTTCTCACTTTGTTGCAGCCGGACGCACCAGTTTTTGCTGATTGCCCCGCACCTCACACAGTTAACAGTTGCTAA

Coding sequence (CDS)

ATGGACTCCGACGTGACGATGGTTCCGGTCGGCGAGGCTTCGAGCAGTGCCGGTCCTTCGTCTACCAAGAAACCCAAGCGTTTTGAGATCAAAAAGTGGAACGCCGTCGCTCTATGGGCATGGGATATCGTTGTCGATAACTGTGCGATCTGCAGAAATCATATCATGGATCTTTGCATCGAGTGCCAAGCAAACCAGGCAAGCGCAACCAGCGAGGAATGTACTGTGGCTTGGGACACAATGTGCTATTATTTTCTACCTCACAACTTACGGATACTGAGGAGGATGTCGAACCATACTGACCTAATCCGTAGGGTGTGTAATCATGCATTTCATTTCCACTGTATCAGTAGATGGTTGAAGACGCGTCAAGTTTGCCCATTGGAAAGCTTTGCCATTTTACAGATAATAGCGAGTGGGAATTTCAGAAGTACGGTCACTAGAACTTTGCTGCTGAGAATATGGATCCTTAAATTCTCATGGGTCTCTAATGTTGTCCCCGATGAACTTTCTCCATACATGGTTTCAGAAATTTGCGATTCGAAGATGCATTTTGTTGAGCAACTACAATTGTCCAGAGAATTATTTTGTGCAGGCAAGGAGTTTGGGATGCCCATTCTTCTCACTTTGTTGCAGCCGGACGCACCAGTTTTTGCTGATTGCCCCGCACCTCACACAGTTAACAGTTGCTAA

Protein sequence

MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWDTMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRWLKTRQVCPLESFAILQIIASGNFRSTVTRTLLLRIWILKFSWVSNVVPDELSPYMVSEICDSKMHFVEQLQLSRELFCAGKEFGMPILLTLLQPDAPVFADCPAPHTVNSC
Homology
BLAST of CmaCh12G013250 vs. ExPASy Swiss-Prot
Match: Q940X7 (RING-box protein 1a OS=Arabidopsis thaliana OX=3702 GN=RBX1A PE=1 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.1e-46
Identity = 93/131 (70.99%), Postives = 98/131 (74.81%), Query Frame = 0

Query: 1   MDSDVTMVPVGEASSS-AGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLC 60
           +DSDVTM+P GEASSS A  SS KK KRFEIKKW+AVALWAWDIVVDNCAICRNHIMDLC
Sbjct: 4   LDSDVTMIPAGEASSSVAASSSNKKAKRFEIKKWSAVALWAWDIVVDNCAICRNHIMDLC 63

Query: 61  IECQANQASATSEECTVAWDTMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRW 120
           IECQANQASATSEECTVAW                           VCNHAFHFHCISRW
Sbjct: 64  IECQANQASATSEECTVAWG--------------------------VCNHAFHFHCISRW 108

Query: 121 LKTRQVCPLES 131
           LKTRQVCPL++
Sbjct: 124 LKTRQVCPLDN 108

BLAST of CmaCh12G013250 vs. ExPASy Swiss-Prot
Match: Q9M2B0 (RING-box protein 1b OS=Arabidopsis thaliana OX=3702 GN=RBX1B PE=2 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 6.5e-40
Identity = 85/130 (65.38%), Postives = 93/130 (71.54%), Query Frame = 0

Query: 1   MDSDVTMVPVGEASSSAGP-SSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLC 60
           ++SDV M   GE+SS + P SS+K  KRFE+KKW+AVALWAWDIVVDNCAICRNHIMDLC
Sbjct: 4   LNSDVIM---GESSSISVPSSSSKNSKRFELKKWSAVALWAWDIVVDNCAICRNHIMDLC 63

Query: 61  IECQANQASATSEECTVAWDTMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRW 120
           IEC ANQASATSEECTVAW                           VCNHAFHFHCISRW
Sbjct: 64  IECLANQASATSEECTVAWG--------------------------VCNHAFHFHCISRW 104

Query: 121 LKTRQVCPLE 130
           LKTRQVCPL+
Sbjct: 124 LKTRQVCPLD 104

BLAST of CmaCh12G013250 vs. ExPASy Swiss-Prot
Match: Q9W5E1 (RING-box protein 1A OS=Drosophila melanogaster OX=7227 GN=Roc1a PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 5.5e-39
Identity = 79/114 (69.30%), Postives = 83/114 (72.81%), Query Frame = 0

Query: 19  PSSTKK--PKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTV 78
           PSS+ K   KRFE+KKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTV
Sbjct: 11  PSSSSKGDKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTV 70

Query: 79  AWDTMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRWLKTRQVCPLES 131
           AW                           VCNHAFHFHCISRWLKTRQVCPL++
Sbjct: 71  AWG--------------------------VCNHAFHFHCISRWLKTRQVCPLDN 98

BLAST of CmaCh12G013250 vs. ExPASy Swiss-Prot
Match: P62877 (E3 ubiquitin-protein ligase RBX1 OS=Homo sapiens OX=9606 GN=RBX1 PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 5.5e-39
Identity = 78/124 (62.90%), Postives = 85/124 (68.55%), Query Frame = 0

Query: 7   MVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQ 66
           M    +  + +G +S    KRFE+KKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQ
Sbjct: 1   MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQ 60

Query: 67  ASATSEECTVAWDTMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRWLKTRQVC 126
           ASATSEECTVAW                           VCNHAFHFHCISRWLKTRQVC
Sbjct: 61  ASATSEECTVAWG--------------------------VCNHAFHFHCISRWLKTRQVC 98

Query: 127 PLES 131
           PL++
Sbjct: 121 PLDN 98

BLAST of CmaCh12G013250 vs. ExPASy Swiss-Prot
Match: P62878 (E3 ubiquitin-protein ligase RBX1 OS=Mus musculus OX=10090 GN=Rbx1 PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 5.5e-39
Identity = 78/124 (62.90%), Postives = 85/124 (68.55%), Query Frame = 0

Query: 7   MVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQ 66
           M    +  + +G +S    KRFE+KKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQ
Sbjct: 1   MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQ 60

Query: 67  ASATSEECTVAWDTMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRWLKTRQVC 126
           ASATSEECTVAW                           VCNHAFHFHCISRWLKTRQVC
Sbjct: 61  ASATSEECTVAWG--------------------------VCNHAFHFHCISRWLKTRQVC 98

Query: 127 PLES 131
           PL++
Sbjct: 121 PLDN 98

BLAST of CmaCh12G013250 vs. ExPASy TrEMBL
Match: A0A6J1HSM1 (RING-box protein 1a-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465785 PE=4 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 7.2e-50
Identity = 101/130 (77.69%), Postives = 103/130 (79.23%), Query Frame = 0

Query: 1   MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 60
           MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI
Sbjct: 1   MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 60

Query: 61  ECQANQASATSEECTVAWDTMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRWL 120
           ECQANQASATSEECTVAW                           VCNHAFHFHCISRWL
Sbjct: 61  ECQANQASATSEECTVAWG--------------------------VCNHAFHFHCISRWL 104

Query: 121 KTRQVCPLES 131
           KTRQVCPL++
Sbjct: 121 KTRQVCPLDN 104

BLAST of CmaCh12G013250 vs. ExPASy TrEMBL
Match: A0A6J1FB79 (RING-box protein 1a-like OS=Cucurbita moschata OX=3662 GN=LOC111444049 PE=4 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 7.2e-50
Identity = 101/130 (77.69%), Postives = 103/130 (79.23%), Query Frame = 0

Query: 1   MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 60
           MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI
Sbjct: 1   MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 60

Query: 61  ECQANQASATSEECTVAWDTMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRWL 120
           ECQANQASATSEECTVAW                           VCNHAFHFHCISRWL
Sbjct: 61  ECQANQASATSEECTVAWG--------------------------VCNHAFHFHCISRWL 104

Query: 121 KTRQVCPLES 131
           KTRQVCPL++
Sbjct: 121 KTRQVCPLDN 104

BLAST of CmaCh12G013250 vs. ExPASy TrEMBL
Match: A0A6P4A6G4 (RING-box protein 1a OS=Ziziphus jujuba OX=326968 GN=LOC107423885 PE=4 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 8.0e-49
Identity = 99/130 (76.15%), Postives = 102/130 (78.46%), Query Frame = 0

Query: 1   MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 60
           +DSDV MVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI
Sbjct: 4   LDSDVPMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 63

Query: 61  ECQANQASATSEECTVAWDTMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRWL 120
           ECQANQASATSEECTVAW                           VCNHAFHFHCISRWL
Sbjct: 64  ECQANQASATSEECTVAWG--------------------------VCNHAFHFHCISRWL 107

Query: 121 KTRQVCPLES 131
           KTRQVCPL++
Sbjct: 124 KTRQVCPLDN 107

BLAST of CmaCh12G013250 vs. ExPASy TrEMBL
Match: A0A6J1DPM4 (RING-box protein 1a-like OS=Momordica charantia OX=3673 GN=LOC111021926 PE=4 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 8.0e-49
Identity = 99/130 (76.15%), Postives = 102/130 (78.46%), Query Frame = 0

Query: 1   MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 60
           +DSDV MVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI
Sbjct: 4   LDSDVPMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 63

Query: 61  ECQANQASATSEECTVAWDTMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRWL 120
           ECQANQASATSEECTVAW                           VCNHAFHFHCISRWL
Sbjct: 64  ECQANQASATSEECTVAWG--------------------------VCNHAFHFHCISRWL 107

Query: 121 KTRQVCPLES 131
           KTRQVCPL++
Sbjct: 124 KTRQVCPLDN 107

BLAST of CmaCh12G013250 vs. ExPASy TrEMBL
Match: M5X2V0 (RING-type domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_2G152700 PE=4 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 1.8e-48
Identity = 98/130 (75.38%), Postives = 102/130 (78.46%), Query Frame = 0

Query: 1   MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 60
           +DSDV MVPVGEASSSAGPSSTK+PKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI
Sbjct: 4   LDSDVPMVPVGEASSSAGPSSTKRPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 63

Query: 61  ECQANQASATSEECTVAWDTMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRWL 120
           ECQANQASATSEECTVAW                           VCNHAFHFHCISRWL
Sbjct: 64  ECQANQASATSEECTVAWG--------------------------VCNHAFHFHCISRWL 107

Query: 121 KTRQVCPLES 131
           KTRQVCPL++
Sbjct: 124 KTRQVCPLDN 107

BLAST of CmaCh12G013250 vs. NCBI nr
Match: KAG6586496.1 (RING-box protein 1a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 245.7 bits (626), Expect = 3.8e-61
Identity = 136/231 (58.87%), Postives = 141/231 (61.04%), Query Frame = 0

Query: 1   MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 60
           MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI
Sbjct: 39  MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 98

Query: 61  ECQANQASATSEECTVAWDTMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRWL 120
           ECQANQASATSEECTVAW                           VCNHAFHFHCISRWL
Sbjct: 99  ECQANQASATSEECTVAWG--------------------------VCNHAFHFHCISRWL 158

Query: 121 KTRQVCPLESFAILQIIASGNFRSTVTRTLLLRIWILKFSWVSNVVPDELSPYMVSEICD 180
           KTRQVCPL+                                                  +
Sbjct: 159 KTRQVCPLD--------------------------------------------------N 187

Query: 181 SKMHFVE-QLQLSRELFCAGKEFGMPILLTLLQPDAPVFADCPAPHTVNSC 231
           S+  F + +L L R      KEFGMPI LTLLQPDAPVFADCPAPHTVNSC
Sbjct: 219 SEWEFQKNRLALKR------KEFGMPIFLTLLQPDAPVFADCPAPHTVNSC 187

BLAST of CmaCh12G013250 vs. NCBI nr
Match: KAF3439695.1 (hypothetical protein FNV43_RR17973 [Rhamnella rubrinervis])

HSP 1 Score: 208.0 bits (528), Expect = 8.7e-50
Identity = 114/185 (61.62%), Postives = 125/185 (67.57%), Query Frame = 0

Query: 1   MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 60
           +DSDV MVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI
Sbjct: 4   LDSDVPMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 63

Query: 61  ECQANQASATSEECTVAWDTMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRWL 120
           ECQANQASATSEECTVAW                           VCNHAFHFHCISRWL
Sbjct: 64  ECQANQASATSEECTVAWG--------------------------VCNHAFHFHCISRWL 123

Query: 121 KTRQVCPLESFAILQIIASGNFRSTVTRTLL-LRIWILKFSWVSNVVPDELSPYMVSEIC 180
           KTR           QI A+GNFRSTVTRT + L  W    + +S       S  +V+ + 
Sbjct: 124 KTR-----------QITANGNFRSTVTRTFMRLSNWASYPALMS-------SRTLVTRVS 144

Query: 181 DSKMH 185
           +S++H
Sbjct: 184 ESRVH 144

BLAST of CmaCh12G013250 vs. NCBI nr
Match: XP_022937741.1 (RING-box protein 1a-like [Cucurbita moschata] >XP_022966069.1 RING-box protein 1a-like isoform X1 [Cucurbita maxima] >XP_023537816.1 RING-box protein 1a-like [Cucurbita pepo subsp. pepo] >KAG7021354.1 RING-box protein 1a [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 207.2 bits (526), Expect = 1.5e-49
Identity = 101/130 (77.69%), Postives = 103/130 (79.23%), Query Frame = 0

Query: 1   MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 60
           MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI
Sbjct: 1   MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 60

Query: 61  ECQANQASATSEECTVAWDTMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRWL 120
           ECQANQASATSEECTVAW                           VCNHAFHFHCISRWL
Sbjct: 61  ECQANQASATSEECTVAWG--------------------------VCNHAFHFHCISRWL 104

Query: 121 KTRQVCPLES 131
           KTRQVCPL++
Sbjct: 121 KTRQVCPLDN 104

BLAST of CmaCh12G013250 vs. NCBI nr
Match: XP_015889019.1 (RING-box protein 1a [Ziziphus jujuba] >XP_022154746.1 RING-box protein 1a-like [Momordica charantia])

HSP 1 Score: 203.8 bits (517), Expect = 1.6e-48
Identity = 99/130 (76.15%), Postives = 102/130 (78.46%), Query Frame = 0

Query: 1   MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 60
           +DSDV MVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI
Sbjct: 4   LDSDVPMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 63

Query: 61  ECQANQASATSEECTVAWDTMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRWL 120
           ECQANQASATSEECTVAW                           VCNHAFHFHCISRWL
Sbjct: 64  ECQANQASATSEECTVAWG--------------------------VCNHAFHFHCISRWL 107

Query: 121 KTRQVCPLES 131
           KTRQVCPL++
Sbjct: 124 KTRQVCPLDN 107

BLAST of CmaCh12G013250 vs. NCBI nr
Match: XP_007218617.1 (RING-box protein 1a [Prunus persica] >XP_008232851.1 PREDICTED: RING-box protein 1a-like [Prunus mume] >XP_008232852.1 PREDICTED: RING-box protein 1a-like [Prunus mume] >XP_034202694.1 RING-box protein 1a-like [Prunus dulcis] >XP_034202695.1 RING-box protein 1a-like [Prunus dulcis] >PQP92865.1 RING-box protein 1a [Prunus yedoensis var. nudiflora] >CAB4269841.1 unnamed protein product [Prunus armeniaca] >ONI22810.1 hypothetical protein PRUPE_2G152700 [Prunus persica] >CAB4300233.1 unnamed protein product [Prunus armeniaca] >VVA19386.1 PREDICTED: RING-box [Prunus dulcis])

HSP 1 Score: 202.6 bits (514), Expect = 3.7e-48
Identity = 98/130 (75.38%), Postives = 102/130 (78.46%), Query Frame = 0

Query: 1   MDSDVTMVPVGEASSSAGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 60
           +DSDV MVPVGEASSSAGPSSTK+PKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI
Sbjct: 4   LDSDVPMVPVGEASSSAGPSSTKRPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCI 63

Query: 61  ECQANQASATSEECTVAWDTMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRWL 120
           ECQANQASATSEECTVAW                           VCNHAFHFHCISRWL
Sbjct: 64  ECQANQASATSEECTVAWG--------------------------VCNHAFHFHCISRWL 107

Query: 121 KTRQVCPLES 131
           KTRQVCPL++
Sbjct: 124 KTRQVCPLDN 107

BLAST of CmaCh12G013250 vs. TAIR 10
Match: AT5G20570.2 (RING-box 1 )

HSP 1 Score: 192.6 bits (488), Expect = 3.5e-49
Identity = 96/134 (71.64%), Postives = 105/134 (78.36%), Query Frame = 0

Query: 1   MDSDVTMVPVGEASSS-AGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLC 60
           +DSDVTM+P GEASSS A  SS KK KRFEIKKW+AVALWAWDIVVDNCAICRNHIMDLC
Sbjct: 4   LDSDVTMIPAGEASSSVAASSSNKKAKRFEIKKWSAVALWAWDIVVDNCAICRNHIMDLC 63

Query: 61  IECQANQASATSEECTVAWD---TMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCI 120
           IECQANQASATSEECTVAW+     C  +       +   S   D +  VCNHAFHFHCI
Sbjct: 64  IECQANQASATSEECTVAWEDDQNNCNKYF-----CILDCSMKDDHLEGVCNHAFHFHCI 123

Query: 121 SRWLKTRQVCPLES 131
           SRWLKTRQVCPL++
Sbjct: 124 SRWLKTRQVCPLDN 132

BLAST of CmaCh12G013250 vs. TAIR 10
Match: AT5G20570.1 (RING-box 1 )

HSP 1 Score: 187.2 bits (474), Expect = 1.5e-47
Identity = 93/131 (70.99%), Postives = 98/131 (74.81%), Query Frame = 0

Query: 1   MDSDVTMVPVGEASSS-AGPSSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLC 60
           +DSDVTM+P GEASSS A  SS KK KRFEIKKW+AVALWAWDIVVDNCAICRNHIMDLC
Sbjct: 4   LDSDVTMIPAGEASSSVAASSSNKKAKRFEIKKWSAVALWAWDIVVDNCAICRNHIMDLC 63

Query: 61  IECQANQASATSEECTVAWDTMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRW 120
           IECQANQASATSEECTVAW                           VCNHAFHFHCISRW
Sbjct: 64  IECQANQASATSEECTVAWG--------------------------VCNHAFHFHCISRW 108

Query: 121 LKTRQVCPLES 131
           LKTRQVCPL++
Sbjct: 124 LKTRQVCPLDN 108

BLAST of CmaCh12G013250 vs. TAIR 10
Match: AT5G20570.3 (RING-box 1 )

HSP 1 Score: 173.3 bits (438), Expect = 2.2e-43
Identity = 92/149 (61.74%), Postives = 97/149 (65.10%), Query Frame = 0

Query: 1   MDSDVTMVPVGEASSS-AGPSSTKKPKRFEIKKWNAVALWAWD----------------- 60
           +DSDVTM+P GEASSS A  SS KK KRFEIKKW+AVALWAW                  
Sbjct: 4   LDSDVTMIPAGEASSSVAASSSNKKAKRFEIKKWSAVALWAWGNLENPYTLSSIGLSMFR 63

Query: 61  -IVVDNCAICRNHIMDLCIECQANQASATSEECTVAWDTMCYYFLPHNLRILRRMSNHTD 120
            IVVDNCAICRNHIMDLCIECQANQASATSEECTVAW                       
Sbjct: 64  YIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWG---------------------- 123

Query: 121 LIRRVCNHAFHFHCISRWLKTRQVCPLES 131
               VCNHAFHFHCISRWLKTRQVCPL++
Sbjct: 124 ----VCNHAFHFHCISRWLKTRQVCPLDN 126

BLAST of CmaCh12G013250 vs. TAIR 10
Match: AT3G42830.1 (RING/U-box superfamily protein )

HSP 1 Score: 165.6 bits (418), Expect = 4.6e-41
Identity = 85/130 (65.38%), Postives = 93/130 (71.54%), Query Frame = 0

Query: 1   MDSDVTMVPVGEASSSAGP-SSTKKPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLC 60
           ++SDV M   GE+SS + P SS+K  KRFE+KKW+AVALWAWDIVVDNCAICRNHIMDLC
Sbjct: 4   LNSDVIM---GESSSISVPSSSSKNSKRFELKKWSAVALWAWDIVVDNCAICRNHIMDLC 63

Query: 61  IECQANQASATSEECTVAWDTMCYYFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRW 120
           IEC ANQASATSEECTVAW                           VCNHAFHFHCISRW
Sbjct: 64  IECLANQASATSEECTVAWG--------------------------VCNHAFHFHCISRW 104

Query: 121 LKTRQVCPLE 130
           LKTRQVCPL+
Sbjct: 124 LKTRQVCPLD 104

BLAST of CmaCh12G013250 vs. TAIR 10
Match: AT3G05870.1 (anaphase-promoting complex/cyclosome 11 )

HSP 1 Score: 55.1 bits (131), Expect = 8.8e-08
Identity = 32/108 (29.63%), Postives = 43/108 (39.81%), Query Frame = 0

Query: 24  KPKRFEIKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWDTMCY 83
           K K   I  W+AVA W WD   + C ICR      C +C+        ++C + W     
Sbjct: 2   KVKILRILLWHAVASWTWDAQDETCGICRMAFDGCCPDCK-----LPGDDCPLIWG---- 61

Query: 84  YFLPHNLRILRRMSNHTDLIRRVCNHAFHFHCISRWL--KTRQV-CPL 129
                                  CNHAFH HCI +W+  +T Q  CP+
Sbjct: 62  ----------------------ACNHAFHLHCILKWVNSQTSQAHCPM 78

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q940X72.1e-4670.99RING-box protein 1a OS=Arabidopsis thaliana OX=3702 GN=RBX1A PE=1 SV=1[more]
Q9M2B06.5e-4065.38RING-box protein 1b OS=Arabidopsis thaliana OX=3702 GN=RBX1B PE=2 SV=1[more]
Q9W5E15.5e-3969.30RING-box protein 1A OS=Drosophila melanogaster OX=7227 GN=Roc1a PE=1 SV=1[more]
P628775.5e-3962.90E3 ubiquitin-protein ligase RBX1 OS=Homo sapiens OX=9606 GN=RBX1 PE=1 SV=1[more]
P628785.5e-3962.90E3 ubiquitin-protein ligase RBX1 OS=Mus musculus OX=10090 GN=Rbx1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HSM17.2e-5077.69RING-box protein 1a-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465785 ... [more]
A0A6J1FB797.2e-5077.69RING-box protein 1a-like OS=Cucurbita moschata OX=3662 GN=LOC111444049 PE=4 SV=1[more]
A0A6P4A6G48.0e-4976.15RING-box protein 1a OS=Ziziphus jujuba OX=326968 GN=LOC107423885 PE=4 SV=1[more]
A0A6J1DPM48.0e-4976.15RING-box protein 1a-like OS=Momordica charantia OX=3673 GN=LOC111021926 PE=4 SV=... [more]
M5X2V01.8e-4875.38RING-type domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_2G152700 ... [more]
Match NameE-valueIdentityDescription
KAG6586496.13.8e-6158.87RING-box protein 1a, partial [Cucurbita argyrosperma subsp. sororia][more]
KAF3439695.18.7e-5061.62hypothetical protein FNV43_RR17973 [Rhamnella rubrinervis][more]
XP_022937741.11.5e-4977.69RING-box protein 1a-like [Cucurbita moschata] >XP_022966069.1 RING-box protein 1... [more]
XP_015889019.11.6e-4876.15RING-box protein 1a [Ziziphus jujuba] >XP_022154746.1 RING-box protein 1a-like [... [more]
XP_007218617.13.7e-4875.38RING-box protein 1a [Prunus persica] >XP_008232851.1 PREDICTED: RING-box protein... [more]
Match NameE-valueIdentityDescription
AT5G20570.23.5e-4971.64RING-box 1 [more]
AT5G20570.11.5e-4770.99RING-box 1 [more]
AT5G20570.32.2e-4361.74RING-box 1 [more]
AT3G42830.14.6e-4165.38RING/U-box superfamily protein [more]
AT3G05870.18.8e-0829.63anaphase-promoting complex/cyclosome 11 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024991Anaphase-promoting complex subunit 11, RING-H2 fingerPFAMPF12861zf-ANAPC11coord: 28..88
e-value: 2.3E-5
score: 24.4
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 15..134
e-value: 2.2E-24
score: 87.5
IPR024766Zinc finger, RING-H2-typePFAMPF12678zf-rbx1coord: 105..128
e-value: 4.6E-11
score: 42.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availablePANTHERPTHR11210RING BOXcoord: 2..130
NoneNo IPR availablePANTHERPTHR11210:SF41E3 UBIQUITIN-PROTEIN LIGASE RBX1coord: 2..130
NoneNo IPR availableCDDcd16485mRING-H2-C3H2C2D_RBX1coord: 46..128
e-value: 1.70355E-35
score: 118.626
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 26..80
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 102..129

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G013250.1CmaCh12G013250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031145 anaphase-promoting complex-dependent catabolic process
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005680 anaphase-promoting complex
molecular_function GO:0097602 cullin family protein binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding